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Global concern revolves around the persistent attributes of harmful non-biodegradable contaminants, especially heavy metals and metalloids, which draws attention to environmental pollution. As regards to soil ecosystem, microorganisms have a significant impact on heavy metal concentrations, either actively or passively. Heavy metal toxicity profoundly impacts the metabolic activities of microbial communities, leading to disruptions in essential cellular processes. Heavy metals interfere with the enzymatic reactions necessary for energy of metabolism, nutrient cycling, and organic matter degradation, resulting in reduced microbial growth and activity. The Biolog Ecoplate method is used as an effective tool for assessing microbial activity. The potential use of Biolog Ecoplate in the study of microbial community structure and diversity in heavy metal-polluted soil was investigated. In order to achieve this goal, multiple keywords combinations were used to filter the most important results in the field. In metal-contaminated soils, the use of Biolog Ecoplate has led to notable changes in microbial community composition, marked by increased populations of resistant and metal-reducing microorganisms.

Pea is one of the most important crop plants (second after soybean) in the Fabaceae family (Shanthakumar et al., 2022) being considered a safe and cheap source of protein, starch, fiber, vitamins and minerals, low-fat, gluten-free, and low-allergenic (Munialo et al. 2014). Leaving the field early and leaving the soil enriched in nitrogen thanks to the symbiosis between the pea roots and Rhizobium spp. bacteria creates ideal conditions for the plants that follow in the rotation. the present paper, we aimed to test the influence of the biological cultivation method on the productivity elements of native yellow pea varieties in the eco-pedological conditions of Transylvania, elaboration of recommendations regarding the applied technology on some morphological and productivity elements in autumn peas. Pea crops were placed in two different locations (SDE Cojocna and private farm in Fundatura), under the same pedoclimatic conditions. In the organic farming system, the Olguța variety showed a reduced number of pods/plants, number of grains in the pod and the highest value of the MMB. In the organic farming system, the most productive pea variety was Ghittia with a production of over 2300 kg/ha and in the conventional farming system Olguța with 5000 kg/ha yield.

Soil microorganisms play a key role in carbon and nutrient cycling processes, which includes the creation and decomposition of soil organic matter (SOM). At the same time, microorganisms use organic C as an energy source, for their development, and to support their functions. Based on our literature search we identified only one model proposed to date for necromass cycle which includes four key stages of the necromass continuum: production, recycling, stabilization, and destabilization. We further scrutinized factors to better understand/explain the processes related to this vital cycle. Microbial necromass is a significant and enduring part of the soil organic carbon (SOC), which is the primary C reservoir in terrestrial ecosystems. Assessing microbial necromass C stocks and understanding how they respond to global changes has become a standard approach in soil C cycle research. Nevertheless, the conventional proxies used to estimate necromass C levels do not provide insights into the dynamic processes and transformations that occur within the soil, ultimately shaping the persistence of microbial necromass.

The purpose of this review is to summarize current possibility of contamination of milk and dairy products and human pathologies as well, that may occur if these products are not processed properly. Scientific literature was consulted to examine the methods of contamination of milk and dairy products. In raw or pasteurized milk stored at 5 °C was not reported that it can grow E. coli, however can grow slowly at higher temperatures. E. coli is considered a high-risk potential pathogen in cheese, due to its unusual ability to tolerate low pH values for long periods and it’s linked to unpasteurized raw milk.

Seed dormancy is a major impediment for the production of Salvia officinalis L. (sage) seedlings. This study aims to test seed priming methods to break the dormancy of sage seeds in controlled conditions. Seeds were subjected to four treatments respectively hydropriming, magneto-priming, electro-priming and control with no seed priming techniques. During germination development stage all parameters were registered according to BBCH (Biologische Bundesanstalt, Bundessortenamt und CHemische Industrie) standardized scale. Daily assessments were made, germination indices were calculated, new germination secondary stages were observed and described, and daily range for each secondary stage were obtained. All priming methods had different effect on sage seeds. The hydropriming treatment was the most effective with 70% seeds germinated in 10 days. Sage seeds from magneto-priming determine more than 50% seeds to germinate in only 8 days. Control and electropriming treatment showed a similar value of seed germination, set around 64-65%. In control, electro- and hydropriming treatments are necessary 6 days for the achievement of more than 50% seed germination, while for magneto-priming are necessary 8 days. Hydropriming has the highest germination index, significantly higher than control and magneto-priming. During the germination, hydropriming shows a gradual transition of seeds from one BBCH sub-stage to another, while electro- and magneto-priming induce a heterogenous variation through the entire process. Overall, the hydropriming treatment registered better results of sage germination compared to the control treatment.

Since the existing information on soils level of fertility in the High Natural Value (HNV) eligible area is scarce, a partnership was concluded between a mixed team of soil scientists and civil society environmentalists within the frame of a research project aiming to: (i) appraise the stakeholders perception and views on the importance of soil conservation and environmental friendly land management, (ii) assess the level of fertility in HNV eligible areas and start building-up the first national HNV soil data base and (iii) identify the most relevant soil indicators for designing an expeditious HNV soil monitoring system. The results revealed the strong agreement of the civil society on the recently issued agricultural policies supporting practices under agri-environment measures and a fair existing level of the soil conservation under HNV practices, as long as land and soil careful administration and resource-efficient management are constantly pursued.

In the current context of global warming, the sustainable development of agriculture can be achieved by expanding the areas cultivated with drought-tolerant plants with high production potential. Sorghum caryopsis, to be intended for human consumption and animal feed, must be free of phytopathogenic agents and meet a series of essential quality conditions. Unfortunately, in unfavourable storage conditions (too high temperature and humidity), caryopsis is attacked by a wide spectrum of pathogens (Fusarium, Alternaria). In this context, the aim of our study is to evaluate the adaptability and productive potential of six sorghum hybrids, identification of pathogens presents on sorghum caryopsis, their quality indices, as well as mycotoxin content. From the data obtained from the notes, observations and analyzes carried out, we can conclude that the percentage of sick caryopses was high in all the hybrids analysed, the microorganisms identified on the caryopses belonged to the genus Fusarium and Alternaria and the mycotoxin content was very low in all the hybrids analysed. This research highlighted the importance of Sorghum which becomes an alternative because it needs only half of the rainfall required by corn (it succeeds even when there is less than 350 mm annual rainfall), being able to replace the corn crop when there are conditions for a poor corn crop.

Heavy metal pollution is a global environmental issue threatening food security and the environment. It is caused by the rapid growth of agriculture and industry. The development of new industries and the increasing number of people have also contributed to the rise in these conditions. Heavy metals that contaminate soils are mercury (Hg), cadmium (Cd), lead (Pb), and chromium (Cr), these toxic substances are retained by the soil and act as a filter for their properties. The aim of this paper was to review the impact of heavy metals on soil, as well as the methods to combat their toxicity in agricultural ones. In order to achieve this goal, data belonging to national and international databases were used (Science Direct, NCBI). The finding of different strategies to combat pollution, particularly on the soil represented the goals for the majority of the studies. As such, bioremediation is a promising choice to reduce heavy metal concentrations.

The aim of this research is to evaluate the ability of Lavandula angustifolia Mill. (LA) to accumulate and translocate heavy metals (HMs) from disturbed soils. The study was conducted on a site historically polluted with HMs, located in Baia Mare, Romania. Soil samples and underground (root) and aboveground parts of plants (leaves and stems) were collected from the study site and analysed for HM content (Pb, Cu, Cd, and Zn) by XRF spectroscopy. The potential for phytoremediation of lavender was evaluated according to the bioconcentration (BCF) and the translocation (TF) factors. The results showed that LA efficiently accumulated Cd and Zn. A different partitioning of HM among plant tissues was observed, showing the highest content in the aboveground mass compared to that found in the root (except for Zn). The TF ranged between 0.62 and 3.59, with values higher than unity for Cd and Pb, revealing that this plant is a suitable candidate for phytoextraction of these two trace elements. According to the BCF values, it seems that lavender is able to bioaccumulate high amounts of Cd (6.66), Pb (1.09), and Zn (2.87). Although more research is necessary for conclusive results, our findings confirm the ability of Lavandula angustifolia Mill. to tolerate, accumulate, and translocate high levels of HMs from soil to the aerial parts of the plant.

Climate change is one of the greatest challenges mankind has ever faced and could lead to potentially devastating global problems, with a need for urgent mitigation and adaptation. Agriculture, especially livestock farming, is a major driver of climate change through its contribution to the total emissions of greenhouse gases (GHGs). The dairy sector has been identified as an important source of GHG emissions, mainly via carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O). In this study, total CO2 equivalent (CO2e) emissions were assessed from a dairy farm (65 dairy cows) located in Romania using the Cool Farm Tool calculator (CFT). We specifically aimed to calculate: (1) the total CO2 equivalent (CO2e) and CO2e per kg FPCM (fat- and protein-corrected milk); (2) methane emissions from enteric fermentation; (3) GHGs resulting from feeding practices; (4) GHGs from manure management; and (5) a simulation of two different scenarios and their impact on GHG emissions. Our results showed annual GHG emissions of 553,170 kg CO2e, almost half of which were released through enteric fermentation. Lactating cows were the major contributor to total GHG emissions, while heifers released the lowest emissions. The two scenarios simulated in this study showed that both the changes made in dairy diet composition and livestock manure management could result in lower GHG emissions. These results confirm the importance and utility of the CFT for the quantification of GHG emissions in dairy farms and its important role as a decision support tool to guide the adoption of good agricultural practices.

Healthy riparian areas are of unique importance for delivering a variety of valuable ecological services. Here, we evaluate to which extend the restoration of urban riparian brownfields could increase the amount of ecosystem services provided by this type of habitat. The research was conducted on a post-industrial site located in Baia Mare city, Romania, historically polluted with heavy metals (HM). The ecosystem services provided by this disturbed ecosystem were assessed according to the list of ecosystem services proposed by the Common International Classification of Ecosystem Services (CICES), and considering the local plant species diversity. Our results showed a limited number of ecosystem services delivered by the disturbed ecosystem analyzed and a species composition mostly formed by non-valuable species. While some groups of plant species identified are native for the riparian habitat, a high percent from species compositions is occupied by the invasive alien species Reynoutria japonica Houtt., impacting the importance and functions of this site. Based on these findings we proposed a list of plant species which could be used in the restoration of this post-industrial ecosystem. This research highlighted the importance of restoration actions on the range of ecosystem services delivered by riparian post-industrial sites.

Most of the ecological experiments conducted so far resulted in limited dissemination and replication of the research results, zero involvement of the citizen in the ecological design, and no educational value of the experiment. Considering these concerns, the aim of our study is to present a novel framework for co-designing urban landscapes polluted with heavy metals (HM). The designed experiments are located inside the city of Baia Mare (Romania), and include 5 brownfields, characterized by different levels of soil HM contamination. At the core of the urban design process stands an interdisciplinary approach which partners scientists from multiple fields related to life sciences (ecologists, agronomists, microbiologists, soil scientists) with urban designers, IT specialists, local municipalities and citizens. The revolutionary approach applied to the ecological and experimental public spaces from Baia Mare city consisted in a co-design process which combined the phytoremediation with future valorization of the biomass produced (via a local environmental bonification system), and the educational value of the ecological experiment.

The roots of the plants are in contact with a substrate, and biotic and abiotic factors influence their functioning and development. The most known microorganisms associated with plant roots are soil fungi, which are adapted to the soil's living environment, called mycorrhizae. Arbuscular mycorrhizae are the most common of these associations. In agricultural ecosystems, the constant use of technologies has resulted in a progressive reduction of mycorrhizal diversity. Due to this phenomenon, soil presents a lower potential for the development of multi-level hyphal networks which implies reduced connections between all individuals of a single species. Mycorrhizas offer benefits for the host plant, including mineral nutrition, increased drought tolerance, and protection against pathogens. They play a significant role in acquiring the necessary nutrients (especially phosphorus and nitrogen) from soil to their host plants, as these nutrients may have low mobility in most soils. Plants take up phosphorus from soil indirectly through the hyphal network that can reach outside from the root depletion area. Mycorrhizal association increases the accumulation of nitrogen in the plant as a result of the competition of hyphae for the organic mineralized form of this element.

Agriculture is one of the key economic activities designed to provide food for a growing population. It is expected that 21st-century agriculture will try to reduce the number of fertilizers by using microorganisms, in this category arbuscular mycorrhizas representing a complex set of benefits for plants and ecosystem services. The aim of this paper is to identify the mycorrhizal structures present in the roots of Zea mays. The objectives of the research are: i) are mycorrhizae natively present in the corn root and have a constant presence from the first stages of plant development? and ii) what kind of colonization pattern is characteristic of these roots? Maize has prominent fasciculate roots, and due to its intense branching capacity provides increased biological support for the establishment of mycorrhizal symbionts. Within the fungal structures highlighted, the most prominent were arbuscules and vesicles. Arum-type arbuscules were mostly observed, Paris-type arbuscules being less common in this species. Vesicles have a low frequency in the root cortex. They are present only in the early stages of plant development. Vesicular-arbuscular mycorrhizae are present in the root of the Zea mays plant with a constant presence, without major fluctuations.

Grasslands are the most dynamic ecosystems are characterized by high biodiversity both above-ground and underground. The biggest problem in these ecosystems is oligotrophy. The phenomenon affects the stability and resilience of these ecosystems, increasing the impact of biological processes. The symbiotic relationship between plants and mycorrhizas is a natural option with a great impact on maintaining the high natural value of ecosystems. This article aims rating colonization Festuca rubra, dominant in a mountain grassland ecosystem. Roots were cleared and stained with ink for the detection of mycorrhizas in roots. All root samples were analyzed with an optical microscope for the detection of mycorrhizal structures. The results obtained highlight a main mycorrhizal pattern as well as specific subcategories. Both types of arbuscules were identified: Arum and Paris. The results obtained highlight a main mycorrhizal pattern as well as specific subcategories. Vesicles are structures with a constant lack of consistency, which indicates their fluctuating nature. The mycorrhizal pattern of Festuca rubra as the dominant species in the pratol ecosystem may be the first step towards a more detailed understanding of the fungal strategy as well as the increased importance in the stability of the pratol ecosystem.

The agricultural sector is currently confronting multifaceted challenges such as an increased food demand, slow adoption of sustainable farming, a need for climate-resilient food systems, resource inequity, and the protection of small-scale farmers’ practices. These issues are integral to food security and environmental health. Remote sensing technologies can assist precision agriculture in effectively addressing these complex problems by providing farmers with high-resolution lenses. The use of vegetation indices (VIs) is an essential component of remote sensing, which combines the variability of spectral reflectance value (derived from remote sensing data) with the growth stage of crops. A wide array of VIs can be used to classify the crops and evaluate their state and health. However, precisely this high number leads to difficulty in selecting the best VI and their combination for specific objectives. Without thorough documentation and analysis of appropriate VIs, users might find it difficult to use remote sensing data or obtain results with very low accuracy. Thus, the objective of this review is to conduct a critical analysis of the existing state of the art on the effective use of VIs for the discrimination and monitoring of several important agricultural crops (wheat, corn, sunflower, soybean, rape, potatoes, and forage crops), grasslands and meadows. This data could be highly useful for all the stakeholders involved in agricultural activities. The current review has shown that VIs appear to be suitable for mapping and monitoring agricultural crops, forage crops, meadows and pastures. Sentinel-1 and Sentinel-2 data were the most utilized sources, while some of the frequently used VIs were EVI, LAI, NDVI, GNDVI, PSRI, and SAVI. In most of the studies, an array of VIs needed to be employed to achieve a good discrimination of crops or prediction of yields. The main challenges in using VIs are related to the variation of the spectral characteristics during the vegetation period and to the similarities of the spectral signatures of various crops and semi-natural meadows. Thus, further studies are needed to establish appropriate models for the use of satellite data that would prove to have greater accuracy and provide more relevant information for the efficient monitoring of agricultural crops.

Agronomic inputs and technologies, especially fertilizers, act on the evolution of the symbiotic partnership between arbuscular mycorrhizal fungi and cultivated plants. The use of the MycoPatt method for the assessment of mycorrhizas in maize roots leads to the extraction of large parameter databases with an increased resolution over the colonization mechanism. The application of a biostimulator treatment on plants acted toward a reduction of root permissiveness for mycorrhizas. The phenomenon was noticeable through an increased colonization variability that overlapped with plant nutritional needs. The annual characteristic of the plant was highlighted by the simultaneous presence of arbuscules and vesicles, with a high share of arbuscules in the advanced phenophases. Colonized root parts presented numerous arbuscule-dominated areas in all phenophases, which indicated a continuous formation of these structures and an intense nutrient transfer between partners. Mycorrhizal maps showed the slowing effect of the biostimulators on colonization, with one phenophase delay in the case of biostimulated plants compared to the ones without biostimulators. The forecast models presented gradual colonization in plants without biostimulators, with the expansion of new hyphal networks. The use of biostimulators on plants exhibited a lower permissiveness for new colonization areas, and the mechanism relies on hyphae developed in the former phenophases.

The paper presents the influence of bacterial inoculation of perennial legumes (alfalfa, bird’s-foot trefoil) in pure culture and cultivated in association with perennial gramineae on fodder yield, fodder quality, and atmospheric nitrogen-fixing ability. Results point out an increase of 10-12% of the yield of dry matter in the variants inoculated. Bacterial inoculation with specific bacterial stems (Sinorhizobium meliloti and Mezorhizobium loti) has a positive influence on fodder quality too, materialised in the raw protein content and the amount of nitrogen fixed biologically.

By this research the authors aimed to analyse the evolution and management of low-input grassland from a specific area in the Apuseni Mountains, in order to define what can be the result of a low input grassland system on the botanical composition of the grasslands and on the income of the farmers. In addition a full description of Flanders area with special attention to its grassland management is given, such that one can make a comparation between those two different cultures: the Province of East Flanders, Belgium and the area of Apuseni Mountains, Cluj County, Romania. Flanders (Belgium) and the area of Cluj County, Apuseni Mountains (Romania), are completely different not only in terms of their people with their own life-styles and culture, but also in terms of uses and management of arable land and grasslands.

Researches carrying evidence for various uses and bioactive principles of Agastache spp. are justifying the upscaling into cultivation of these medicinal species. But, hindrances in their cultivation exist due to the insufficient documentation of their biology under field conditions. Because productivity of these medicinal species (herba) is ensured by the combined contribution of plant agronomic traits, these are related to the feasibility of the crop and therefore, can be used as predictors for successful cultivation. The aim of this study was to evaluate comparatively four valuable Agastache species (A. mexicana, A. scrophulariifolia, A. foeniculum) and one cultivar (A. rugosa ‘After Eight’), in order to identify the favourability for cultivation in local conditions (Romania). Based on the structural indicators of plant morphology (plant height, shoot number, leaf number, leaf length and width, inflorescence length, verticillaster number and flower number), registered over the span of two years, were explored relationships and similarities as well as their implications in previsioning the phenotypic potential. The results showed that studied species acclimatized successfully and all agronomic parameters studied increased in values in the second year. The average plant height in second year (2020) was 109.8 cm and average inflorescences length 9.6 cm. Stable positive correlations between inflorescence length with plant height and shoot number were observed, while differences among species became pronounced as plants become established, evidenced by clearer distinction in the second year. Phenotypic potential in the absence of inputs enables the feasibility assessment for medicinal plants introduced for cultivation in new regions.

Reynoutria japonica Houtt (RJ) is an extremely invasive plant species, found nowadays in a wide range of habitats, including those polluted with heavy metals (HM). The aim of this study was to investigate HM dynamics in RJ–soil interactions in five habitats historically polluted with HM located in Baia Mare city, Romania. The concentrations of major metal elements (Cd, Cu, Pb, Zn) in plant tissues (roots, stems, leaves) and soil samples collected from the study sites were analyzed via portable ED-XRF spectroscopy (converted), and the translocation factor (TF) and bioconcentration factor (BCF) were calculated. The mean values of HM in soil samples collected from the study sites exceeded the threshold limit values established by Romanian legislation. Generally, the highest concentration of Cd was recorded in the above-ground part of the plant (stem and leaves), while for Cu, Pb and Zn, the highest values (with few exceptions) were recorded in the root. The metal transfer was highly effective from soil to RJ, such that all four of the HM studied exceeded the normal range of metals in a plant. Analysis of metal concentrations in plant tissues showed an efficient movement of Cd and Zn to the above-ground parts of the plant, a tendency particularly pronounced in the case of Cd (TF and BCF > 1), while Pb was the least bioaccumulated HM. It may be concluded that RJ is able to tolerate high concentrations of HM, being a good phytoextractor for Cd and Zn.

Soil salinization processes have increased over the years and affect large parts of agricultural fields. The purpose of this review was to highlight the most important aspects regarding the potential effects of soil salinity on plants. In the current context of climate change, extreme weather and increased drought periods can lead to plant metabolic dysfunctionalities and accumulation of salt ions because of the increasing need for irrigation. The most important limiting factor, salinity, has a highly negative impact on plant growth independent of the appearance of either natural or anthropic status. The negative aspects include decreased leaf development rate, a low water level in all parts of the plant, reduced cell division and elongation, and low-intensity photosynthetic rate. Other negative aspects are directly related to stomata closure, reduced transpiration, low CO2 level, and limitations on seed germination. However, there are also some positive aspects to the presence of salinity in soil. The field offers unlimited possibilities of research in order to activate pathways that help plants become resistant to salt stress. Several physiological parameters can benefit from low salt concentration (halopriming), such as germination, vigor, rapid seedling growth, and increased stomata number. Further studies should focus on both the positive and negative aspects of the increase in soil salinity.

Germination of the medicinal and aromatic plant basil raises interesting questions due to its no seed periodicity and dormancy demand, and the seed priming could help to assure the permanent needs for this herb. The study aimed to provide an adapted BBCH (Biologische Bundesanstalt Bundessortenamt and CHemical industry) scale for the germination secondary stages of sweet basil Ocimum basilicum L. (var. MVSt). A standardized germination test was performed with hydropriming, electric field, and magnetic field as priming methods to assess the changes in the secondary stages of germination. The time range for each basil secondary stage was recorded and added to the adapted BBCH, to highlight the daily secondary stage changes. All the seed priming methods increased the germination capacity with 2–3% compared with the control, with the highest value for electropriming. The seed priming methods studied improved the germination and changed the pattern of secondary stages in the BBCH scale. The germination energy was set to more than 90% in all primed seeds, with a maximum of 91.75% in the magnetic field. The time needed for 50% germination of seeds was set to 6.5 days and 90% germinated seeds were recorded after 11.7 days. The 09a BBCH lasted for 9 days in control and hydropriming, 10 days in electric field conditions and 7 days for magnetoprimed seeds.

Abstract The harmonization of methodologies for the assessment of radicular endophytic colonization is a current necessity, especially for the arbuscular mycorrhizas. The functionality of mycorrhizal symbionts for plants can be described only by indicators obtained based on microscopic analysis. That is the reason for which a unifying methodology will lead to the achievement of highly correlated indicators comparable from one research to another. Our proposed methodology can further digitize the microscopic observations of colonization. The MycoPatt system is developed as a methodological framework for obtaining objective and comparable microscopic observations. The horizontal, vertical and transversal indicators are highly adaptable and allow the tracking of mycorrhizal colonization in root length. All structures developed by symbionts can be traced and the obtained metadata can be compared without any transformation. Mycorrhizal maps have a high degree of applicability in evaluating soil inoculum efficiency. In the future, the application of this method will lead to digital maps with a high degree of accuracy. MycoPatt allows the mathematical expression of colonization patterns, being a complex model that converts biological data into statistically comparable indicators. This will further allow obtaining inferences with applicative importance and similarity spectra for the colonizing fungi and host plants.

Quality of Dutch iris bulbs produced in field conditions is important for obtaining marketable cut flowers by forcing in greenhouse. Natural occurring symbiotic micromycetes can facilitate important nutrient acquisition for plants with short vegetative cycle such as geophytes. In an open field experiment established in April 2018 in Cluj county Romania, was evaluated susceptibility of some Iris × hollandica cultivars to colonization by arbuscular mycorrhizae fungi in first stages of rooting. Average number of entry points per cm root length was 11 after twelve days in field. Density increased with second centimetre from root apex. Arbuscular mycorrhizae infection sites on roots before advanced proliferation occurs can be used as indicator both for soil mycorrhizal potential as well as plant genotype affinity to these symbiotic fungi.

Iris plants are widely cultivated flowering ornamentals, with a long history of traditional use in Eurasia, where this genus is reaching the highest diversity. This paper aims to provide an overview on recent advances related to the phytoremediation potential of plants from the genus Iris, in order to promote the use of these species in phytoremediation programs. According to the relevant literature, eight species from genus Iris present phytoremediation potential (I. dichotoma, I. germanica, I. halophila, I. lactea, I. latifolia, I. pseudacorus, I. sibirica, I. wilsonii). The studies addressed potential of plants to mitigate toxic metals/metalloids (As, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Zn), excess of nutrients (P, N), pesticides, petroleum hydrocarbons, pharmaceuticals as well as dyes. Most studies focused on wastewater treatment and environments contaminated due to mining activities. Main hindrances in upscaling this green technology remain mitigation of toxicity stress in plants during remediation and the disposal of resulting contaminated biomass. In this sense, use of beneficial microorganisms to alleviate phytotoxicity effects and new valorization possibilities of contaminated Iris spp. biomass have been proposed recently. Designing an entire cycle that includes phytoremediation and sustainable value chains for contaminated biomass could prove feasible and should receive more attention.

Long-term fertilization targets mycorrhizal fungi adapted to symbiotic exchange of nutrients, thus restricting their colonization potential and re-orienting the colonization strategies. The MycoPatt tool has a high applicability in quantifying the symbiotic process with the identification of mycorrhizal indices and projection of mycorrhizal patterns. Organic treatments increase the symbiotic process, visible in values of colonization frequency and intensity, with about 6% more than the native status of colonization. At the opposite pole, organic-mineral treatments decrease the colonization parameters by up to half of the organic treatment. All of the colonization parameters show significant correlations, except for the arbuscules/vesicle ratio (0.03). All the applied treatments, except for the organic one, record multiple root segments with a colonization degree lower than 10%. The application of treatments changes the strategy of native colonization from a transfer (40%) and storage (37%) to a predominant storage (50%) for organic treatment, and are mainly proliferative between 38–50% in mixed and mineral treatments. The high amount of mineral components increases also the presence of resistance conditions strategies. The use of mycorrhizal pattern maps, with the inclusion of colonization strategies, presents an important direction in understanding the evolution of mutual relations, and to explore in-depth the efficiency of the whole symbiotic process.

Arbuscular mycorrhizal fungi (AMF) are beneficial for plant development and help absorb water and minerals from the soil. The symbiosis between these fungi and plant roots is extremely important and could limit crop dependence on fertilizers. The aim of this study was to evaluate the influence of AMF on tomatoes (Solanum lycopersicum L.), based on important agronomic traits of vegetative biomass, production, and fruits. The experiment was conducted in high tunnels, using 12 tomato genotypes under three different treatments: T1, control, without fertilizer and mycorrhizae colonization; T2, fertigation, without mycorrhizae colonization; and T3, arbuscular mycorrhizal fungi (AMF), seedling roots being inoculated with specialized soil-borne fungi. Plant growth, yield and fruit parameters indicated better results under mycorrhizal treatment. Root colonization with fungi varied significantly depending on the treatment and genotype, with a variation of 6.0–80.3% for frequency and 2.6–24.6% for intensity. For a majority of characteristics, the mycorrhization (T3) induced significant differences compared with the T1 and T2 treatments. In addition, AMF treatment induced a different response among the genotypes. Among the elements analyzed in the soil, significant differences were observed in phosphorous levels between planting the seedlings and after tomato harvesting and clearing of the plants. The results suggest that reducing fertilizers and promoting the symbiotic relationships of plants with soil microorganisms may have beneficial consequences for tomato crops.

Medicinal and aromatic plants hold a high share of interest in Romania. To offset the harvesting of spontaneous flora and ensure the sustainable conservation of natural resources, the cropping of highly valuable plants of interest represents a priority for the current agricultural system. This study was conducted due to the need for more balance in species exploitation. Therefore, it assessed the emergence dynamics, leaf appearance, growth and development of the plants depending on row spacing and plant distance. The research was conducted at the INCDCSZ Brașov, within the Technology Department, Laboratory of Medicinal and Aromatic Plants. The density of planting is the most important factor. Plants sown in continuous rows showed the highest yields of fresh and dry herbs, especially when the distance between the rows was set to 25 cm. Row-by-row distances of 50 cm ensure average values for all growth and development parameters. An increase in the row-by-row distance up to 70 cm drastically reduced the dry biomass up to 7.89 t ha−1 in continuous rows, followed by a 2 and 2.5 t ha−1 decrease for 15 cm and 25 cm plant-by-plant distances, respectively. Continuous row planting and a 25 cm row-by-row distance negligibly reduce the climate impact on growth and development. Greater spaces between plants leads to an average increase in individual development, but with a decrease in the total potential yield.

Exploring grassland microbiomes is a challenge in the current context of linking soil microorganism activity with the balance of these ecosystems. Microbiologists are constantly attempting to develop faster and lower-cost methods, and propose new and best-fitted indicators that will provide a more complex data analysis. A different concept was proposed for assessing functional microbiomes by splitting the functional ecological niche into complementary segments. The comparison with the upper and lower limits of the ecological niche provides a clearer image of community alterations due to long-term applied treatments. The method allows the extraction of the most sensitive and stable functional guilds, with the extraction of the most critical dominant–codominant functional groups in every segment of the functional niche. The resulting microbial functional–sociological model is ready to use on community-level physiological profile databases and also can be applied backward for vegetation analysis.

Festuca rubra is one of the dominant species in mountain high natural values grasslands. Most herbaceous plants are involved in a symbiotic partnership with arbuscular mycorrhizas for maintaining their abundance and cover. This research was conducted to explore the changes in mycorrhizal colonization patterns and structure development due to the long-term application of treatments. A large database of microscopic images was deeply analyzed with the MycoPatt tool, for the extraction of clear mycorrhizal maps that present particular colonization strategies. The overall colonization frequency and intensity varied largely between treatments, with a maximum in organic conditions. The presence of both arbuscules and vesicles in the same root area indicate a continuous alternance of fungal strategy, from storage to enhanced transfer of nutrients. A low-mineral organic treatment permits a clear separation of colonization strategy in different parts of roots. The nutrient availability due to mineral treatment induced a restriction in mycorrhizal development, which was maintained colonization by a resistance conditions strategy. The use of mycorrhizal maps permits a deep scanning of colonized roots, identifying the real positioning of fungal structures, along with their developmental potential and the assessment of the colonization strategy.

Although essential for achieving high crop yields required for the growing population worldwide, nitrogen, (N) in large amounts, along with its inefficient use, results in environmental pollution and increased greenhouse gas (GHG) emissions. Therefore, improved nitrogen use efficiency (NUE) has a significant role to play in the development of more sustainable crop production systems. Considering that wheat is one of the major crops cultivated in the world and contributes in high amounts to the large N footprint, designing sustainable wheat crop patterns, briefly defined by us in this review as the 3 Qs (high quantity, good quality and the quintessence of natural environment health) is urgently required. There are numerous indices used to benchmark N management for a specific crop, including wheat, but the misunderstanding of their specific functions could result in an under/overestimation of crop NUE. Thus, a better understanding of N dynamics in relation to wheat N cycling can enhance a higher efficiency of N use. In this sense, the aim of our review is to provide a critical analysis on the current knowledge with respect to wheat NUE. Further, considering the key traits involved in N uptake, assimilation, distribution and utilization efficiency, as well as genetics (G), environment (E) and management (M) interactions, we suggest a series of future perspectives that can enhance a better efficiency of N in wheat.

Mycorrhiza develop on the surface of the roots and create complex networks of nutrients transport between the hosts. The present paper evaluates the modification of endomycorrhizal parameters in urban grasses due to the distance from the trunk of the trees. Grass samples were harvested from 3 parks. The objective is to identify the area of maximum development of the mycorrhizal network and the spatial stability of the symbiosis. A pattern of mycorrhizal mechanisms can be identified due to human intervention in the ecosystem. In ecosystems, the overhead distance between trees is dominated by grassy species capable of endomycorrhizal connections. Between fungi and plants, connecting nodes and nutrient transfer points appear. Frequency of mycorrhizas in grasses is over 90%. The intensity in root is below 30%, but supported by high arbuscularity. The impact of mycorrhizae is felt only at the time of intense colonization and the presence of arbuscules.

Mineral and organic fertilization have a huge effect on natural grasslands. The purpose of this research is to assess the state of the biodiversity and pastoral value for grasslands from the Transylvanian Plateau area. In this area has been analyzed the floristic composition and a series of ecological indexes, respectively humidity, soil reaction, temperature and nitrogen. Other aspects taken in account were the agronomical and anthropogenic specters. The analyzed grasslands are placed in the perimeter of Gheorgheni village, from Cluj County. Experience includes 20 experimental variants with organic fertilization with combined with mineral fertilization, 5 variants in 4 rehearsals. Each experimental variant is 2 m long X 5 m wide. In most hill meadows the economic efficiency is relatively low, and in order to be increased, it is necessary to apply the whole complex of measures for their improvement, care and exploitation, of which a special role is the application of appropriate treatments that stimulate the development of valuable species. Several researches show that applying fertilizers on grasslands is economically justified since, generally, 1 kg of active element results in an increase of 80-100 kg of green matter.

Urban parks are ecosystems with a dynamic directed by human intervention. Arbuscular mycorrhizal fungi are symbionts with higher plants, with role in increasing the amount of nutrients needed to plant development. In roots, the fungi develops various structures for transfer or storage of nutrients. The grasses developed in the parks of the Cluj-Napoca city present typical mycorrhizal structures, including the two morphotypes of arbuscules. The colonization rate identified in the analyzed samples is over 75%, indicating a high number of propagules in soil. The dependence of arbuscles is higher to intensity than colonization frequency.

MicroResp is a colorimetric method developed by Campbell et al., (2003), used for assessing the community level physiological profile of the microbial population. MicroResp can be used to assess soil health, pollution induced community tolerance, also for toxicity testing, pesticide degradation profiles, bioremediation evaluation and water ecology and toxicity. The aim of the present review was to look over the results of recent papers and to highlight the importance and efficiency of the MicroResp method in assessing the physiological profile of the microbial community. The method advantages and limitations were also assessed. We focused on agricultural soil in order to deepen our understanding about changes of microbial community induced by agricultural practices. To achieve this goal, academic literature was analyzed using an academic database. There were set a total of six keywords, used to make a search algorithm, achieving five search terms. For each search, the first four articles of interest were chosen to be reviewed. After the searches for each of the terms, between 72 and 210 articles were found, 20 of them being chosen for final evaluation. Following the undertaken research, it can be stated that MicroResp method is an important tool to assess the physiological profile of the microbial community, featuring a series of advantages that place it ahead of other competing methods.

Soybean yields could benefit from the use of improved varieties, nutrients and rhizobium inoculants. In this study we evaluated the influence of fertilization with liquid manure upon soybean yield in the 3rd experimental year, in climatic condition specific to the year 2016. The experiences were installed on Agricultural Research-Development Station Turda, on a chernozem soil. We followed the response of two soybean varieties to organic fertilization with liquid manure. In this regard we analyzed the number of nodules at the beginning and at the end of flowering and bean production. The results pointed out that in general Onix genotype recorded the highest values on bean production when 15 t/ha liquid manure were applied.

Grassland ecosystems occupy significant areas worldwide and represent a reservoir for biodiversity. These areas are characterized by oligotrophic conditions that stimulate mycorrhizal symbiotic partnerships to meet nutritional requirements. In this study, we selected Festuca rubra for its dominance in the studied mountain grassland, based on the fact that grasses more easily accept a symbiotic partner. Quantification of the entire symbiosis process, both the degree of colonization and the presence of a fungal structure, was performed using the root mycorrhizal pattern method. Analysis of data normality indicated colonization frequency as the best parameter for assessing the entire mycorrhizal mechanism, with five equal levels, each of 20%. Most of the root samples showed an intensity of colonization between 0 and 20% and a maximum of arbuscules of about 5%. The colonization degree had an average value of 35%, which indicated a medium permissiveness of roots for mycorrhizal partners. Based on frequency regression models, the intensity of colonization presented high fluctuations at 50% frequency, while the arbuscule development potential was set to a maximum of 5% in mycorrhized areas. Arbuscules were limited due to the unbalanced and unequal root development and their colonizing hyphal networks. The general regression model indicated that only 20% of intra-radicular hyphae have the potential to form arbuscules. The colonization patterns of dominant species in mountain grasslands represent a necessary step for improved understanding of the symbiont strategies that sustain the stability and persistence of these species.

Excessive application of chemical fertilizers and other agrochemicals can cause large imbalances in soils and agricultural ecosystems. In this context, mycorrhizae represent a viable solution to mitigate these negative effects. Arbuscular mycorrhizae are vital symbionts due to the multiple benefits they bring to both crops and the entire agroecosystem. The main purpose of this study was to observe whether differentiated fertilization has an influence on mycorrhizal colonization patterns in corn. Observed frequencies and intensities of colonization varied widely between phenophases and treatments, with 20% variation for frequency and 14% for intensity, which implies the constant development of both partners during the vegetation period. Arbuscules and vesicles were present in all development stages, but the overall mean was lower than 4% for arbuscules and 1% for vesicles in the analyzed root fragments. Intensity was highly correlated with frequency of colonization compared with arbuscules, where the coefficient was 0.54, and vesicles, with a coefficient of 0.16. Both PCA and NMDS provided good graphical solutions, with a high resolution due to explained variance and good spatial position of vectors. The use of mycorrhizal maps permits the full exploration of colonization patterns and fungal strategy, and the assessment of mycorrhizae-free areas. For the untreated variant, the strategy was oriented toward a longitudinal colonization followed by an irregular development of hyphae with multiple non-colonized areas. Treatment acts to stimulate the appearance of mycorrhizal spots, which further develop radially.

Microbial groups developed in the rhizosphere ensure the stability and dynamics of nutrient transfer processes to plants. Additional soil inoculation with rhizospheric bacteria acts to expand the ecological niche, supplements the activity, the number of individuals and promote plant growth. In today’s agriculture, there is a need for the usage of rhizosphere microflora and integrative approaches to stimulate plant growth. The aim of the study is to evaluate the use of biofertilizers as starter stimulators in autumn wheat. Different concentrations of Bactofil, containing rhizospheric bacteria, were applied in small rhizothrones. Evaluation of relationships between bacterial inoculum and plant reveals the degree of applicability and adaptation to the soil conditions of exogenous microflora. Doses of 15.6 x 108 and 20.8 x 108 cells/m2 stimulates the root elongation and higher level of mycorrhization. Bioproducts can be viable solutions to stimulate the initial development of wheat plants.

Agricultural practices, such as the use of fertilizers, can change the structure and function of soil microbial community. Monitoring and assessing the soil microbiota and its dynamics related to different factors can be a powerful tool for understanding basic and applied ecological contexts. The main objective of this paper was to assess the changes of carbon turnover rate and the microbial metabolic activity, when different types of fertilizers were used, process called priming effect. A microcosm experiment was designed and performed under controlled temperature and humidity and the soil samples were analyzed using the MicroResp technique. Results show that the integration in soil of different carbon sources, such as green manure, can lead to a positive priming effect and integration of mineral fertilizers can lead to negative priming effect. The carbon sources with the highest respiratory activity were α-ketoglutaric acid, malic acid, oxalic acid, citric acid, while the lowest respiratory activity was obtained in case of arginine.

Water ecosystems are constantly under the pressure of a high number of biotic and abiotic factors, and their action leads to the occurrence of fluctuations in species and populations. The specific response of organisms to external pressures leads to the reorganization of ecosystems, with qualitative and quantitative changes. The aim is to identify the microbial groups with the highest potential in the waste waters. Data were collected during 2015-16 in Bistrita. Identifying the bioindicators quality of microorganisms and their importance in ecosystems was done by comparing them with international databases. The use of microorganisms as bioindicators involves knowing the requirements for one or more environmental variables. The concept refers to the ability of microorganisms to accumulate pollutants. Flagellates are tolerant to acidity and react quickly and with sensitivity to environmental stress by inhibiting their movement. The multiplication of flagellates and filamentous bacteria is manifested through water disturbance and foam production.

Semi-natural grasslands are particularly important in mountainous areas of Romania, being the only source of forage for many farmers. The aim of this study was to investigate the changes in forage quantity and quality as a result of Urea Ammonium nitrate (UAN) liquid fertilization. The experiment was carried out in the eastern part of Apuseni Mountains, Romania on a Festuca rubra L.-Agrostis capillaris L. grassland located at 1240 m altitude. Studies were made over three years of experimental trial (2014–2016) and covered four experimental plots in three replicates, as follows: V1–control plot, unfertilized; V2–plot fertilized with 50 kg UAN ha−1 year−1; V3–plot fertilized with 75 kg UAN ha−1 year−1, and V4–plot fertilized with 100 kg UAN ha−1 year−1. The experimental plots were harvested once per year and the botanical composition, dry matter yield and forage quality were assessed. Our results showed important changes in forage quantity, quality and diversity as a result of UAN fertilization. Starting from the second experimental year the dominance/co-dominance ratio changed favoring the species from Poaceae family. Dry matter increased as a result of UAN fertilization but forage quality was negatively affected by the higher percentage of participation of species from other botanical families which have higher crude fiber content and lower crude protein. Based on our results we recommend moderate fertilization with UAN up to 50 kg UAN ha−1 year−1 for semi-natural grasslands located in soil-climatic conditions similar to those in our experiment.

The purpose of this research is to assess the state of the biodiversity and pastoral value for Festuca rupicola- Bothriochloa ischaemum grasslands from the hill area. Also, there has been analyzed the floristic composition and a series of ecological indexes, respectively humidity, soil reaction, temperature and nitrogen. Other aspects taken in account were the agronomical and anthropogenic specters. The analyzed grasslands are placed in the perimeter of the localities: Gilău, Aiton. Jucu and Frata, all from Cluj County.

Floristic composition of the pastures is a mirror action and practical factors applied station and management (maintenance and how to use). The purpose of this research is to assess the state of the biodiversity and pastoral value for Agrostis capillaries L. grasslands from the mountain area. Also, there has been analyzed the floristic composition and a series of ecological indexes, respectively humidity, soil reaction, temperature, and nitrogen. We examined how estimated species diversity patterns changed with varying survey intensity from Băișoara village, Cluj County, Romania, an Agrostis capillaries L. grasslands. The experiment was performed in 2015. The vegetation observations were made on 29 plots. In this descriptive plot been identified the type of grassland Agrostis capillaries, described in boreal at altitudes between 850 and 1200, generally starting on exhibitions east until the south (90˚ - 180 ˚).The floristic composition of the Agrostis capillaries grasslands from the studied area is influenced by the local conditions and the intensity of the exploitation, these surfaces being under-exploited.

Plant rhizosphere is the portion of soil which is in direct contact with the plant roots. From the microbiological point of view, this area is characterized by strong dynamic of functional groups with high specificity towards the substrate available. Spring barley is a crop with high requirements to the composition of the microflora in the rhizosphere, disturbances produced by agronomic inputs affecting the stability of rhizospheric contact interfaces and ultimately the plant growth. Analysis of changes within the microbial community was carried out with the purpose of defining the disruptive impact of mineral inputs and potential of zeolite to reduce these disruptions. Microbial functional groups were analyzed on the basis of the CO2 export under the specific conditions of soil inoculation on specific substrates over a time period of incubation. Microresp detection plates allow evaluation of a large number of samples under identical conditions of inoculation and the establishment of dynamics of the entire microbial community. The dynamics of the entire microbial communities (basal respiration) is stimulated to increase in case of unilateral application of zeolite and zeolite as a buffer for urea fertilization. General growth trend of microbial communities follows proportional the associated application of zeolite with urea, the most powerful non-symbiotic nitrogen fixation processes being stimulated by this combination of fertilizers. Simultaneously, an increase in the dynamics of denitrifiers was observed, also the decomposition of lignin and cellulose and biological crust formation due to the proliferation of cyanobacteria. Rhizosphere of barley plants is characterized by the presence of actinomycetes as dominant in functional microbial community of all experimental variants analyzed with a high capacity for biological degradation and raised mineralization of organic matter.

An important part of efficient livestock production is ensuring the sufficient grass for hay and pasture. However, low soil nutrient levels often limit forage production. With good fertilizer management and soil fertility, the productivity of many hay and pasture fields can be greatly improved. Through good fertilizer management, the productivity of many hay and pasture fields can be significantly improved by Ross H. McKenzie (2005). The aim of this paper was the effect of fertilization with liquid fertilizer (UAN) the harvest of dry and floristic composition changing on natural grassland. The experiment whose results we present was placed in 2014 in the place in Baisoara Mountain village, Cluj County. Experience has been placed on the Festuca rubra and Agrostis capillaries - of grassland type. The natural grassland of Festuca rubra with Agrostis capillaries responded very well to mineral fertilizers with liquid fertilizer UAN. The floristic composition of natural grassland fertilized with liquid fertilizers based on nitrogen, it can be seen an increase the Gramineae families and an evidence downward trend from Fabaceae families.

Microbial communities in agricultural ecosystems are characterized by a strong dynamic and radical change due to technological inputs applied. Corn is cultivated on large areas with high requirements for nutrients and an increased potential for activation of specific microbial groups. The aim of this study was to assess the unilateral and synergic effect of zeolite and mineral fertilizers on the development and transformation of microbial functional groups in the rhizosphere of corn. Physiological profile assessment of microbial communities has been carried out on the basis of substrate induced respiration, monitored over a period of 6 hours of incubation. The amount of CO2 registered in Microresp plates represents the activity of functional groups in decomposition of each type of substrate applied. Characteristic groups of microorganisms in maize rhizosphere are capable of decomposing acids: citric, L-malic, oxalic and α-Ketoglutaric. These substrates indicate the presence of high concentrations of organic matter in soil and the existence of a biological crust on the surface (citric acid), respectively the existence of powerful processes for the decomposition of organic material by actinomycetes (α-Ketoglutaric acid). The highest microbial activities were observed in groups of bacteria involved in processes of plant growth promotion and microbial groups with an important role in the processes of denitrification (oxalic acid). For the application of urea a triple value of activity of this type of microflora is observed. Functional groups codominant in soils cultivated with corn are specialized in efficient degradation of organic matter and biological crust, zeolite providing the complex substrate necessary for the development of these microorganisms.

This paper presents karyology aspects of the diploid and polyploid species frequency on the mountain pastures in the southwestern Gutai Mountains as well as the calculation of the diploid index. The phytocoenosis of the five plant associations identified on pasture were studied: Violo declinatae-Nardetum, Simon 1966, Agrostietum stoloniferae, (Ujvárosi 1941) Burduja et al. 1956, Scirpetum sylvatici, Ralski 1931, Maloch 1935 em. Schwick 1944, Calthetum laetae, Krajina 1933, Campanulo abientinae-Vaccinietum myrtilli, (Buia et al. 1962) Boşcaiu 1971. Karyotypes were taken for all encountered vegetal species to achieve an accurate cytoaxonomic analysis. The frequency of polyploid species was found in the five vegetal associations that were identified and studied in karyology terms. The number of polyploids, comprised between 52.94-75%, is ahead of the diploids number comprised between 25-43,75%, in the vegetal associations phytocoenosis of the mountainous region. Diplo-polyploid plants and those with an unknown karyotype have lower participation. The calculated value of the diploid index decreases with the increasing of the altitude, with subunit values between 0.33 - 0.82. Diploid index (DI) indicates instable character of vegetal formations of above-mentioned area, reflecting completely station conditions of the grassland. The genetic structure of the phytocoenosis and vegetal associations studied seen from the perspective of karyotypes, indicates a drop in the dioloid index value with the increasing of the anthropo- zoogen factor (excessive grazing).

Grassland productivity is a complex attribute defined by a series of interdependent indices with important role in the characterization of one grassland culture productivity. Among this the most important are the soil-climatic conditions, the technology applied, floristic composition, forage quality, etc. The present paper aims to follow the behaviour of a forage mixture with red clover among fourth experimental year. We followed the behaviour of a complex mixture consisting of Trifolium pratense (15%), Lolium perenne (20%), Festulolium (25%), Festuca arundinacea (25%) and Phleum pratense (15%) under the influence of mineral fertilization in the climatic conditions specific Plateau of Transylvania, Romania. Experience was installed in the spring of 2012 in experimental fields located inside the Agricultural Research and Development Station Turda. The experimental area is characterized by an average annual temperature of 10.40C C and average annual rainfall of 523.2 mm. Soil type is faeoziom vertic clay. The mixture was sown on 12.5 cm and 25 cm distances between rows and they were fertilized in early March with complex NPK and ammonium nitrate (33.3%) in four different doses: V1- control variant, unfertilized, V2-N50P60K80, V3- N75P60K80 and V4-N100P60K80. The experimental parcels were mowed 3 times /year. Analysing the complex forage mixture we observed an interesting evolution among the fourth experimental years. Fertilization with medium amounts of nitrogen seemed to have a favourable influence on forage mixture productivity. The results showed that the forage mixture studied can generate high productivity and quality if adequate technology is applied.