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25 results on '"Rovera, Marisa"'

1. Inoculation of tomato roots with single and mixed suspensions of Trichoderma harzianum ITEM 3636 conidia and Pseudomonas putida PCI2 cells.

Author

Pastor, Nicolás A., Cejas, Leonardo G., Guiñazú, Lorena B., Rovera, Marisa, and Torres, Adriana M.

Subjects
TOMATOES, PSEUDOMONAS putida, TRICHODERMA harzianum, CONIDIA, PLANT colonization, FRUIT yield, SCANNING electron microscopy, BACTERIAL colonies
Abstract

Mixes of compatible beneficial microorganisms can perform better than single strains to support plant health and growth. This study examined effects of inoculating tomato (Solanum lycopersicum L.) roots with individual and mixed suspensions of Trichoderma harzianum ITEM 3636 and Pseudomonas putida PCI2 on growth and yield in field assays. Culture filtrates were used to observe in vitro compatibility between strains. Effects of ITEM 3636 on tomato seedlings in vitro were studied. Scanning electron microscopy was used to examine root colonization by the bacterial and fungal strains. ITEM 3636 was compatible with PCI2 and improved root density in vitro. When compared to other treatments, co-inoculation of tomato plants consistently resulted in increases in the number of fruit and yield under field conditions. Co-inoculating tomato roots during transplanting with the studied microorganisms may improve plant survival and increase productivity. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Difundiendo virología ¿contagiando ciencia?

Author

Sartori, Melina Victoria, Torres, Cristina Vanesa, Rovera, Marisa, de la Barrera, María Laura, Torres, Cesar Gastón, Pollo, Alejandro, Ciani, Marcelo G., Vogliotti, Ana, Ledesma, María Luisa, Roldán, Carolina Isabel, and Clerici, Jimena Vanina

Subjects
Innovaciones pedagógicas, purl.org/becyt/ford/5 [https], Educación superior, Competencias, purl.org/becyt/ford/5.3 [https], Enseñanza
Abstract

El presente trabajo se enmarca en la convocatoria de Proyectos de Innovación e Investigación para el Mejoramiento de la Enseñanza deGrado (PIIMEG) 2017-2018. Partimos de trabajar interdisciplinariamente la problemática relacionada con potenciar las competencias de los futuros profesionales para que no solo dominen el conocimiento en Microbiología, y en particular en Virología, sino también puedan promoverlo, movilizarlo y transferirlo a través de cierto impacto social de ese accionar. Se torna esencial en la vinculación entre la ciencia y la sociedad el protagonismo de un público que se constituye como actor esencial en la prevención de la salud. Prevalece sobre todo una responsabilidad social de los científcos en sus hallazgos. La habilidad de seleccionar información, redactar, elaborar informes, mediar, resolver conflictos, trabajar en equipo, en ambientes multiculturales, planifcar y organizar son algunas de las características de un divulgador que contribuirán a la consolidación de determinadas competencias.Su estímulo por parte de los mismos formadores en los estudiantes de carreras de esta índole permitirá generar un compromiso social y una responsabilidad en el desarrollo de su profesión. De hecho, mostramos en este trabajo una manera diferente de divulgar, prevenir y compartir contenidos puntuales en torno a la virología. Fil: Sartori, Melina Victoria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Torres, Cristina Vanesa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Rovera, Marisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: de la Barrera, María Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Humanas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Torres, Cesar Gastón. Universidad Nacional de Río Cuarto; Argentina Fil: Pollo, Alejandro. Universidad Nacional de Río Cuarto; Argentina Fil: Ciani, Marcelo G.. Universidad Nacional de Río Cuarto; Argentina

Published
2019

16. Potential biological control pseudomonas sp. PCI2 against damping-off of tomato caused by Sclerotium Rolfsii

Author

Pastor, Nicolás Alejandro, Reynoso, Maria Marta, Tonelli, Maria Laura, Masciarelli, Oscar Alberto, Rosas, Susana Beatriz, and Rovera, Marisa

Subjects
purl.org/becyt/ford/1 [https], FLUORESCENT PSEUDOMONAS, SCLEROTIUM ROLFSII, purl.org/becyt/ford/1.6 [https], TOMATO PLANTS, BIOLOGICAL CONTROL
Abstract

Fluorescent Pseudomonas spp. isolated from the roots of healthy tomato plants were screened for their antagonistic activities against Sclerotium rolfsii, Alternaria alternata and Fusarium solani, three phytopathogenic fungi of tomato and pepper. They were tested for phosphate solubilization ability and production of siderophores, hydrolytic enzymes, indole 3-acetic acid and hydrogen cyanid. The isolates were also characterized based on biochemical (API 20NE test) and genotypic (ERIC-PCR fingerprinting) features. A Pseudomonas sp. strain denoted PCI2 was chosen as a potential candidate for controlling tomato damping-off caused by Sclerotium rolfsii. PCI2 was identified at the genus level with a 16S rDNA partial sequence analysis and its phylogenetic relationship with previously characterized Pseudomonas species was determined. PCI2 clustered with the P. putida species. Growth chamber studies resulted in statistically significant increases in plant stand (29%) as well as in root dry weight (58%). PCI2 was able to establish itself and survive in tomato rhizosphere after 40 days, following planting of bacterized seeds. PCI2 is a potential biological control agent that may contribute to the protection of tomato plants against damping-off caused by S. rolfsii. Fil: Pastor, Nicolás Alejandro. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Reynoso, Maria Marta. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Tonelli, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Masciarelli, Oscar Alberto. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Rosas, Susana Beatriz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Rovera, Marisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina

Published
2010

24. Evaluation of Pseudomonas chlororaphis subsp. aurantiaca SR1 for growth promotion of soybean and for control of Macrophomina phaseolina.

Author

Rovera, Marisa, Pastor, Nicolás, Niederhauser, Marina, and Rosas, Susana B.

Subjects
*PSEUDOMONAS, *PLANT growth, *SOYBEAN, *MACROPHOMINA phaseolina, *BIOLOGICAL pest control agents, *ANTIFUNGAL agents, *HYDROCYANIC acid
Abstract

Pseudomonas chlororaphissubsp.aurantiacaSR1 was evaluated for control ofMacrophomina phaseolina in vitroand in soybean plants, for growth promotion of soybean plants and for production of antifungal compounds. Strain SR1 caused a significant inhibition ofM. phaseolina in vitroand reduced damping-off in thein vivoassays. In addition, strain SR1 significantly increased shoot and root length and shoot and root dry weight of soybean plants inM. phaseolinainfested soil, as compared to control plants in infested soil. Fragments for the phenazine-1-carboxylic acid, pyrrolnitrin and hydrogen cyanide encoding genes were amplified from the DNA of strain SR1 after polymerase chain reaction (PCR) assays with specific primers. Thus, this study establishes thatP. chlororaphissubsp.aurantiacaSR1 provides control ofM. phaseolina in vivoand suggests that strain SR1 might be applied as an effective biocontrol agent to protect soybean plants from this phytopathogen. [ABSTRACT FROM AUTHOR]

Published
2014
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25. Photoinactivation of Escherichia coli using porphyrin derivatives with different number of cationic charges

Author

Spesia, Mariana B., Lazzeri, Débora, Pascual, Liliana, Rovera, Marisa, and Durantini, Edgardo N.

Subjects
ESCHERICHIA coli, PORPHYRINS, CATIONS, ESCHERICHIA
Abstract

Abstract: The photodynamic effect of meso-substituted cationic porphyrins, 5-[4-(trimethylammonium)phenyl]-10,15,20-tris(2,4,6-trimethoxyphenyl)porphyrin iodide 1, 5,10-di(4-methylphenyl)-15,20-di(4-trimethylammoniumphenyl)porphyrin iodide 2 and 5-(4-trifluorophenyl)-10,15,20-tris(4-trimethylammoniumphenyl)porphyrin iodide 3, have been investigated in both homogeneous medium bearing photooxidizable substrates and in vitro on a typical gram-negative bacterium Escherichia coli. Absorption and fluorescence spectroscopic studies were compared in N,N-dimethylformamide. Fluorescence quantum yields (ϕF) of 0.10, 0.06 and 0.08 were calculated for porphyrins 1, 2 and 3, respectively. The singlet molecular oxygen, O2(1Δg), production was evaluated using 9,10-dimethylanthracene yielding values of 0.66, 0.36 and 0.42 for porphyrins 1, 2 and 3, respectively. Guanosine 5′-monophosphate was used as biological substrate model. Similar decomposition of guanosine 5′-monophosphate was obtained using these cationic porphyrins as sensitizer. In biological medium, photosensitized inactivation of E. coli was analyzed using cells without and with one washing step. E. coli cultures were treated with sensitizer at 37°C for 30min in dark. In both procedures, a higher photoinactivation of cells (>99.999%) was found for cells treated with 10μM of tricationic porphyrin 3 and irradiated for 5min with visible light. Porphyrins 1 and 2 only show an important photodamage when the cells are irradiated without washing step. These results indicated that the tetracationic porphyrin 3 could be a promising sensitizer with potential applications in the photoinactivation of bacterial cells by photodynamic therapy. [Copyright &y& Elsevier]

Published
2005
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