23 results on '"Roujia Wang"'
Search Results
2. Adaptive Design of Fluorescence Imaging Systems for Custom Resolution, Fields of View, and Geometries
- Author
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Roujia Wang, Riley J. Deutsch, Enakshi D. Sunassee, Brian T. Crouch, and Nirmala Ramanujam
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Medical technology ,R855-855.5 ,Biotechnology ,TP248.13-248.65 - Abstract
Objective and Impact Statement: We developed a generalized computational approach to design uniform, high-intensity excitation light for low-cost, quantitative fluorescence imaging of in vitro, ex vivo, and in vivo samples with a single device. Introduction: Fluorescence imaging is a ubiquitous tool for biomedical applications. Researchers extensively modify existing systems for tissue imaging, increasing the time and effort needed for translational research and thick tissue imaging. These modifications are application-specific, requiring new designs to scale across sample types. Methods: We implemented a computational model to simulate light propagation from multiple sources. Using a global optimization algorithm and a custom cost function, we determined the spatial positioning of optical fibers to generate 2 illumination profiles. These results were implemented to image core needle biopsies, preclinical mammary tumors, or tumor-derived organoids. Samples were stained with molecular probes and imaged with uniform and nonuniform illumination. Results: Simulation results were faithfully translated to benchtop systems. We demonstrated that uniform illumination increased the reliability of intraimage analysis compared to nonuniform illumination and was concordant with traditional histological findings. The computational approach was used to optimize the illumination geometry for the purposes of imaging 3 different fluorophores through a mammary window chamber model. Illumination specifically designed for intravital tumor imaging generated higher image contrast compared to the case in which illumination originally optimized for biopsy images was used. Conclusion: We demonstrate the significance of using a computationally designed illumination for in vitro, ex vivo, and in vivo fluorescence imaging. Application-specific illumination increased the reliability of intraimage analysis and enhanced the local contrast of biological features. This approach is generalizable across light sources, biological applications, and detectors.
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- 2023
- Full Text
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3. S100a9 deficiency accelerates MDS-associated tumor escape via PD-1/PD-L1 overexpression
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Roujia Wang, Youshan Zhao, Zijuan Li, Juan Guo, Sida Zhao, Luxi Song, Dong Wu, Lan Wang, and Chunkang Chang
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Biophysics ,General Medicine ,Biochemistry - Published
- 2023
4. Bone Marrow Fibrosis at Diagnosis and during the Course of Disease Is Associated with TP53 Mutations and Adverse Prognosis in Primary Myelodysplastic Syndrome
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Youshan Zhao, Juan Guo, Sida Zhao, Roujia Wang, Lei Shi, Ying Fang, Zheng Zhang, Luxi Song, Dong Wu, and Chunkang Chang
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Cancer Research ,Oncology ,myelodysplastic syndrome ,bone marrow fibrosis ,TP53 ,prognosis - Abstract
The prognostic significance of bone marrow fibrosis (MF) grade in patients with myelodysplastic syndrome (MDS) is still debated and the molecular changes remain unclear. In our large cohort, a normal reticulum was found in 211 (25.9%) patients, whereas MF1, MF2 and MF3 were detected in 478 (58.7%), 90 (11.1%) and 35 (4.3%) patients at initial diagnosis, respectively. Patients with MF often correlated with some poor prognostic characteristics, including older age, anemia, unfavorable karyotype, higher BM blast and a higher IPSS-R category. For the entire cohort, the median OS was not reached, 30, 16 and 15 months for patients with MF 0, 1, 2 and 3, respectively. After adjusting for IPSS-R, the hazard ratio for mortality was 1.56 (95% CI, 1.18–2.06) for patients with MF1, 2.29 (95% CI, 1.61–3.27) for patients with MF2 and 2.75 (95% CI, 1.69–4.49) for patients with MF3 compared with those with MF0. The mutational landscape of 370 patients showed that TP53, U2AF1 and KMT2D mutations were more frequent in patients with MF2-3. In addition, of the 408 patients with MF0-1, 62 patients (15.1%) progressed to MF2-3 during the follow-up interval. The clinical features, mutation landscape and prognosis of patients with progressed fibrosis were similar to those of patients with MF2-3 at diagnosis. We concluded that BM fibrosis (MF1, 2 and 3) was an adverse prognosis feature in primary MDS and correlated with TP53 mutations both at the time of initial diagnosis and during the course of the disease. Therefore, BM fibrosis should be included in the revised prognostic scoring system and carefully considered in treatment selection.
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- 2022
5. Targeting UHRF1-SAP30-MXD4 axis for leukemia initiating cell eradication in myeloid leukemia
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Cheng-Long Hu, Bing-Yi Chen, Zijuan Li, Tianbiao Yang, Chun-Hui Xu, Ruirui Yang, Peng-Cheng Yu, Jingyao Zhao, Ting Liu, Na Liu, Bin Shan, Qunling Zhang, Junhong Song, Ming-Yue Fei, Li-Juan Zong, Jia-Ying Zhang, Ji-Chuan Wu, Shu-Bei Chen, Yong Wang, Binhe Chang, Dan Hou, Ping Liu, Yilun Jiang, Xiya Li, Xinchi Chen, Chu-Han Deng, Yi-Yi Ren, Roujia Wang, Jiacheng Jin, Kai Xue, Ying Zhang, Meirong Du, Jun Shi, Ling-Yun Wu, Chun-Kang Chang, Shuhong Shen, Zhu Chen, Sai-Juan Chen, Xiaolong Liu, Xiao-Jian Sun, Mingyue Zheng, and Lan Wang
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Leukemia, Myeloid, Acute ,Carcinogenesis ,Ubiquitin-Protein Ligases ,CCAAT-Enhancer-Binding Proteins ,Humans ,Cell Biology ,Molecular Biology ,Histone Deacetylases - Abstract
Aberrant self-renewal of leukemia initiation cells (LICs) drives aggressive acute myeloid leukemia (AML). Here, we report that UHRF1, an epigenetic regulator that recruits DNMT1 to methylate DNA, is highly expressed in AML and predicts poor prognosis. UHRF1 is required for myeloid leukemogenesis by maintaining self-renewal of LICs. Mechanistically, UHRF1 directly interacts with Sin3A-associated protein 30 (SAP30) through two critical amino acids, G572 and F573 in its SRA domain, to repress gene expression. Depletion of UHRF1 or SAP30 derepresses an important target gene, MXD4, which encodes a MYC antagonist, and leads to suppression of leukemogenesis. Further knockdown of MXD4 can rescue the leukemogenesis by activating the MYC pathway. Lastly, we identified a UHRF1 inhibitor, UF146, and demonstrated its significant therapeutic efficacy in the myeloid leukemia PDX model. Taken together, our study reveals the mechanisms for altered epigenetic programs in AML and provides a promising targeted therapeutic strategy against AML.
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- 2022
6. Development of a fluorescence illumination optimization algorithm for mobile imaging applications in resource limited settings
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Riley Deutsch, Roujia Wang, Brian T. Crouch, and Nimmi Ramanujam
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- 2022
7. Simultaneous determination of 11 illicit drugs and metabolites in wastewater by UPLC-MS/MS
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Wei Liu, Yan Shi, Ruxin Luo, Ping Xiang, Roujia Wang, Xin Wang, Baohua Shen, Shuai Yuan, and Zhiguo Yu
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China ,Environmental Engineering ,Wastewater ,010501 environmental sciences ,Mass spectrometry ,Tandem mass spectrometry ,01 natural sciences ,Tandem Mass Spectrometry ,medicine ,Solid phase extraction ,0105 earth and related environmental sciences ,Water Science and Technology ,Detection limit ,Chromatography ,Illicit Drugs ,Chemistry ,Solid Phase Extraction ,010401 analytical chemistry ,Extraction (chemistry) ,Codeine ,0104 chemical sciences ,Sewage treatment ,Water Pollutants, Chemical ,Chromatography, Liquid ,medicine.drug - Abstract
Assessing collective drug consumption based on the concentrations of illicit drugs and their metabolites in wastewater is a new technology. Currently, this technology is receiving attention in China, and methods for multiple illicit drug detection in wastewater are urgently needed. In our study, a method with a short runtime (7 min), a small solid-phase extraction (SPE) loading volume (50 mL) and high sensitivity (lower limits of quantitation (LLOQs) ranged from 0.2 to 5 ng/L) was developed for the simultaneous determination of amphetamines, ketamine, opiates, cocaine and their metabolites in wastewater. Samples were enriched by SPE on a mixed-mode sorbent (Oasis MCX) and analyzed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The limits of detection (LODs) ranged from 0.1 to 2 ng/L, and the LLOQs varied between 0.2 and 5 ng/L. Moreover, the method developed was applied to real wastewater samples collected from 15 different wastewater treatment plants (WWTPs). In the results, the most abundant compounds were morphine (1.8–46.6 ng/L) and codeine (3.7–24.9 ng/L), which were detected in 13 WWTPs. After successful optimization of the UPLC-MS/MS conditions and sample loading pH, the method developed is able to meet the needs of common illicit drug monitoring and high-throughput analysis requirements.
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- 2020
8. Pulmonary Infection Within 100 Days After Transplantation Impaired Platelet Recovery in Patients with Hematologic Malignancies: A Propensity-Score-Matched Analysis
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Lei Gao, Jianmin Yang, Libing Wang, Roujia Wang, Xiong Ni, Weiping Zhang, Xiaoxia Hu, Huiying Qiu, Qi Chen, Aijie Huang, and Jianmin Wang
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Adult ,Blood Platelets ,Male ,medicine.medical_specialty ,Platelet Engraftment ,medicine.medical_treatment ,Hematopoietic stem cell transplantation ,Infections ,Gastroenterology ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Humans ,Medicine ,Platelet ,Young adult ,Risk factor ,Propensity Score ,Aged ,Retrospective Studies ,Original Paper ,Transplantation ,Platelet Count ,business.industry ,Hematopoietic Stem Cell Transplantation ,Retrospective cohort study ,Pneumonia ,General Medicine ,Middle Aged ,medicine.disease ,Treatment Outcome ,Hematologic Neoplasms ,030220 oncology & carcinogenesis ,Female ,business ,030215 immunology - Abstract
BACKGROUND Pulmonary infection is one of the life-threatening complications occurring during allogeneic hematopoietic stem cell transplantation (alloHSCT), even when prophylactic measures have been employed. Few studies have investigated whether pulmonary infection affects platelet recovery during alloHSCT. MATERIAL AND METHODS We retrospectively reviewed 253 consecutive patients with hematologic diseases who received alloHSCT in our institute. Among them, 62 patients (25%) had pulmonary infection within 100 days after alloHSCT. Using the one-to-two propensity-score matching logistic model, 50 patients with pulmonary infection and 100 patients without were included based on age, disease and stage, time from diagnosis to transplantation, infused CD34⁺ cells, and mononuclear cells. RESULTS The incidences of prolonged thrombocytopenia in patients with pulmonary infection were 44% (22/50) and 9% (9/100) in the corresponding matched group (P
- Published
- 2019
9. Investigation of the mechanism of incompatible herb pair gansui-gancao-induced hepatotoxicity and nephrotoxicity and the attenuated effect of gansuibanxia decoction by UHPLC-FT-ICR-MS-based plasma metabonomic analysis
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Ruiyun Li, Nan Zhang, Yue Cui, Fei Han, Zhiguo Yu, Ye Zhang, Yunli Zhao, Roujia Wang, and Ting Liu
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Male ,food.ingredient ,Clinical Biochemistry ,Pharmaceutical Science ,Decoction ,Traditional Chinese medicine ,Asteraceae ,01 natural sciences ,Analytical Chemistry ,Nephrotoxicity ,Drug Incompatibility ,Rats, Sprague-Dawley ,food ,Drug Discovery ,Herb pair ,Animals ,Humans ,Metabolomics ,Chromatography, High Pressure Liquid ,Spectroscopy ,Traditional medicine ,010405 organic chemistry ,Chemistry ,010401 analytical chemistry ,Acute Kidney Injury ,Rats ,0104 chemical sciences ,Disease Models, Animal ,Herb ,Ft icr ms ,Potential biomarkers ,Toxicity ,Chemical and Drug Induced Liver Injury ,Biomarkers ,Drugs, Chinese Herbal - Abstract
Gansui-Gancao is one of the "eighteen incompatible herb pairs" which was recorded 2000 years ago according to TCM (Traditional Chinese Medicine) theory for their toxicity when using together. Nevertheless, Gansuibanxia decoction contained the herb pair have satisfactory effect on the treatment of cancerous ascites, pericardial effusion, etc. The present study aimed to investigate the mechanism of the incompatibility of Gansui-Gancao and the compatibility of Gansuibanxia decoction using UHPLC-FT-ICR-MS in a metabonomic perspective. Rats were divided into four groups administrated with different herb combination extracts for successive 14 days. Orthogonal partial least squares-discriminant analysis (OPLS-DA) was used to plot the metabolic state and screen the potential biomarkers in plasma. A total of 20 biomarkers contributed to the separation of Gansui-Gancao group and control group were tentatively identified mainly involved in 7 metabolic pathways related to hepatotoxicity and nephrotoxicity. The contents of these biomarkers were adjusted to normal levels in Gansuibanxia decoction group. Thus, the results of our study reveled the mechanism of the incompatibility of Gansui-Gancao and the compatibility of Gansuibanxia decoction in a metabonomic perspective and it's valuable for better understanding the "eighteen incompatible madicaments" of TCM theory.
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- 2019
10. Exploiting heat shock protein expression to develop a non-invasive diagnostic tool for breast cancer
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Nirmala Ramanujam, Brian T. Crouch, Timothy A.J. Haystead, Roujia Wang, Joy Duer, Mary Scott Soo, Allison Hall, Jennifer Gallagher, and Philip F. Hughes
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0301 basic medicine ,Gene Expression ,Estrogen receptor ,lcsh:Medicine ,Breast Neoplasms ,Ligands ,Article ,Hsp90 inhibitor ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Cell Line, Tumor ,Heat shock protein ,Biopsy ,Biomarkers, Tumor ,medicine ,Humans ,HSP90 Heat-Shock Proteins ,Stage (cooking) ,Receptor ,lcsh:Science ,Heat-Shock Proteins ,Multidisciplinary ,medicine.diagnostic_test ,Tumor-infiltrating lymphocytes ,business.industry ,Cell Membrane ,Optical Imaging ,lcsh:R ,medicine.disease ,Immunohistochemistry ,Molecular Imaging ,3. Good health ,030104 developmental biology ,Molecular Diagnostic Techniques ,ROC Curve ,Cancer research ,Female ,lcsh:Q ,business ,030217 neurology & neurosurgery - Abstract
Leveraging the unique surface expression of heat shock protein 90 (Hsp90) in breast cancer provides an exciting opportunity to develop rapid diagnostic tests at the point-of-care setting. Hsp90 has previously been shown to have elevated expression levels across all breast cancer receptor subtypes. We have developed a non-destructive strategy using HS-27, a fluorescently-tethered Hsp90 inhibitor, to assay surface Hsp90 expression on intact tissue specimens and validated our approach in clinical samples from breast cancer patients across estrogen receptor positive, Her2-overexpressing, and triple negative receptor subtypes. Utilizing a pre-clinical biopsy model, we optimized three imaging parameters that may affect the specificity of HS-27 based diagnostics – time between tissue excision and staining, agent incubation time, and agent dose, and translated our strategy to clinical breast cancer samples. Findings indicated that HS-27 florescence was highest in tumor tissue, followed by benign tissue, and finally followed by mammoplasty negative control samples. Interestingly, fluorescence in tumor samples was highest in Her2+ and triple negative subtypes, and inversely correlated with the presence of tumor infiltrating lymphocytes indicating that HS-27 fluorescence increases in aggressive breast cancer phenotypes. Development of a Gaussian support vector machine classifier based on HS-27 fluorescence features resulted in a sensitivity and specificity of 82% and 100% respectively when classifying tumor and benign conditions, setting the stage for rapid and automated tissue diagnosis at the point-of-care.
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- 2019
11. Understanding the sources of errors in
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Roujia, Wang, Daniel A, Alvarez, Brian T, Crouch, Aditi, Pilani, Christopher, Lam, Caigang, Zhu, Philip, Hughes, David, Katz, Timothy, Haystead, and Nirmala, Ramanujam
- Subjects
Article - Abstract
Overexpression of heat shock protein 90 (Hsp90) on the surface of breast cancer cells makes it an attractive molecular biomarker for breast cancer diagnosis. Before a ubiquitous diagnostic method can be established, an understanding of the systematic errors in Hsp90-based imaging is essential. In this study, we investigated three factors that may influence the sensitivity of ex vivo Hsp90 molecular imaging: time-dependent tissue viability, nonspecific diffusion of an Hsp90 specific probe (HS-27), and contact-based imaging. These three factors will be important considerations when designing any diagnostic imaging strategy based on fluorescence imaging of a molecular target on tissue samples.
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- 2020
12. In Vivo Optical Metabolic Imaging of Long-Chain Fatty Acid Uptake in Orthotopic Models of Triple-Negative Breast Cancer
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Andrei Goga, Joy Duer, Matthew D. Hirschey, Joyce V. Lee, Nirmala Ramanujam, Megan C. Madonna, Baris Avsaroglu, Brian T. Crouch, Riley Deutsch, Caigang Zhu, Roujia Wang, and Jeremy Williams
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0301 basic medicine ,Cancer Research ,lcsh:RC254-282 ,fluorescence microscopy ,Article ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,In vivo ,oncogene addition ,medicine ,Triple-negative breast cancer ,chemistry.chemical_classification ,Mammary tumor ,Oncogene ,breast murine tumor lines ,In vitro toxicology ,Fatty acid ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Transport inhibitor ,fatty acid uptake ,030104 developmental biology ,Oncology ,chemistry ,030220 oncology & carcinogenesis ,metastatic potential ,Cancer research ,metabolism - Abstract
Targeting a tumor&rsquo, s metabolic dependencies is a clinically actionable therapeutic approach, however, identifying subtypes of tumors likely to respond remains difficult. The use of lipids as a nutrient source is of particular importance, especially in breast cancer. Imaging techniques offer the opportunity to quantify nutrient use in preclinical tumor models to guide development of new drugs that restrict uptake or utilization of these nutrients. We describe a fast and dynamic approach to image fatty acid uptake in vivo and demonstrate its relevance to study both tumor metabolic reprogramming directly, as well as the effectiveness of drugs targeting lipid metabolism. Specifically, we developed a quantitative optical approach to spatially and longitudinally map the kinetics of long-chain fatty acid uptake in in vivo murine models of breast cancer using a fluorescently labeled palmitate molecule, Bodipy FL c16. We chose intra-vital microscopy of mammary tumor windows to validate our approach in two orthotopic breast cancer models: a MYC-overexpressing, transgenic, triple-negative breast cancer (TNBC) model and a murine model of the 4T1 family. Following injection, Bodipy FL c16 fluorescence increased and reached its maximum after approximately 30 min, with the signal remaining stable during the 30&ndash, 80 min post-injection period. We used the fluorescence at 60 min (Bodipy60), the mid-point in the plateau region, as a summary parameter to quantify Bodipy FL c16 fluorescence in subsequent experiments. Using our imaging platform, we observed a two- to four-fold decrease in fatty acid uptake in response to the downregulation of the MYC oncogene, consistent with findings from in vitro metabolic assays. In contrast, our imaging studies report an increase in fatty acid uptake with tumor aggressiveness (6NR, 4T07, and 4T1), and uptake was significantly decreased after treatment with a fatty acid transport inhibitor, perphenazine, in both normal mammary pads and in the most aggressive 4T1 tumor model. Our approach fills an important gap between in vitro assays providing rich metabolic information at static time points and imaging approaches visualizing metabolism in whole organs at a reduced resolution.
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- 2020
13. Quantitative assessment of distant recurrence risk in early stage breast cancer using a nonlinear combination of pathological, clinical and imaging variables
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Brandon S. Nichols, Lee G. Wilke, Rachel A. Greenup, Amanda Schulman, Erika Chelales, Joseph Geradts, Nirmala Ramanujam, Roujia Wang, Paul K. Marcom, Jennifer Gallagher, and Josephine Harter
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Oncology ,medicine.medical_specialty ,General Physics and Astronomy ,Breast Neoplasms ,Risk Assessment ,01 natural sciences ,Article ,General Biochemistry, Genetics and Molecular Biology ,010309 optics ,Correlation ,Breast cancer ,Internal medicine ,0103 physical sciences ,medicine ,Humans ,General Materials Science ,Breast density ,Stage (cooking) ,Pathological ,Neoplasm Staging ,Cancer staging ,business.industry ,010401 analytical chemistry ,Distant recurrence ,General Engineering ,General Chemistry ,medicine.disease ,0104 chemical sciences ,Cohort ,Neoplasm Recurrence, Local ,business - Abstract
Use of genomic assays to determine distant recurrence risk in patients with early stage breast cancer has expanded and is now included in the American Joint Committee on Cancer staging manual. Algorithmic alternatives using standard clinical and pathology information may provide equivalent benefit in settings where genomic tests, such as OncotypeDx, are unavailable. We developed an artificial neural network (ANN) model to nonlinearly estimate risk of distant cancer recurrence. In addition to clinical and pathological variables, we enhanced our model using intraoperatively determined global mammographic breast density (MBD) and local breast density (LBD). LBD was measured with optical spectral imaging capable of sensing regional concentrations of tissue constituents. A cohort of 56 ER+ patients with an OncotypeDx score was evaluated. We demonstrated that combining MBD/LBD measurements with clinical and pathological variables improves distant recurrence risk prediction accuracy, with high correlation (r = 0.98) to the OncotypeDx recurrence score. This article is protected by copyright. All rights reserved.
- Published
- 2020
14. UHPLC-MS/MS method for simultaneously detecting 16 tryptamines and their metabolites in human hair and applications to real forensics cases
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Yan Shi, Huosheng Qiang, Zhiguo Yu, Olaf H. Drummer, Shuai Yuan, Roujia Wang, Baohua Shen, Min Shen, Yunli Zhao, and Ping Xiang
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Adult ,Male ,Formic acid ,Clinical Biochemistry ,030226 pharmacology & pharmacy ,01 natural sciences ,Biochemistry ,High-performance liquid chromatography ,Analytical Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Young Adult ,0302 clinical medicine ,Limit of Detection ,Tandem Mass Spectrometry ,Humans ,Chromatography, High Pressure Liquid ,Detection limit ,Chromatography ,Chemistry ,Elution ,010401 analytical chemistry ,Hair analysis ,Reproducibility of Results ,Cell Biology ,General Medicine ,Forensic Medicine ,Middle Aged ,Tryptamines ,0104 chemical sciences ,Solvent ,Linear Models ,Ammonium acetate ,Hair - Abstract
Tryptamines are hallucinogenic substances many of which have appeared recently as novel psychoactive substances (NPS). Herein, we describe the establishment of a rapid UHPLC-MS/MS quantitative method for the targeted screening of 16 tryptamines of abuse in hair. Twenty milligram pieces of hair were pulverized below 4 °C in 0.5 mL of deionized water containing 0.1% formic acid and an internal standard (2 ng/mL psilocin-d10 and psilocybin-d4). After subsequent centrifugation, 5 μL of the supernatant was injected into a LC–MS/MS system fitted with a Waters Acquity UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm). The column was gradient eluted at 0.3 mL/min with mobile phases composed of 20 mmol/L ammonium acetate, 5% acetonitrile, and 0.1% formic acid in water (solvent A) and acetonitrile (solvent B). Limits of detection ranged between 0.1 and 20 pg/mg, with limits of quantitation ranging from 3 to 50 pg/mg. The calibration curves for all analytes were linear (r > 0.992). Accuracies varied between 91% and 114%, with intraday precision RSDs
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- 2020
15. Leveraging Surface Hsp90 Expression for Rapid-on-site Breast Cancer Diagnostics
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Timothy A.J. Haystead, Brian T. Crouch, Daniel Adrianzen Alvarez, Christopher T. Lam, Nimmi Ramanujam, Riley Deutsch, and Roujia Wang
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Materials science ,biology ,medicine.disease ,Signal ,Hsp90 ,Fluorescence ,Breast cancer ,Heat shock protein ,Fluorescence microscope ,medicine ,biology.protein ,Molecular imaging ,Diffusion kinetics ,Biomedical engineering - Abstract
We developed a portable, low-cost, handheld fluorescent microscope to perform rapid heat shock protein 90 (Hsp90) molecular imaging. The source of non-specific signal and diffusion kinetics of the probe is studied.
- Published
- 2020
16. Understanding the sources of errors in ex vivo Hsp90 molecular imaging for rapid-on-site breast cancer diagnosis
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Roujia Wang, Nirmala Ramanujam, Brian T. Crouch, Caigang Zhu, Timothy A.J. Haystead, David F. Katz, Aditi Pilani, Daniel Adrianzen Alvarez, Philip F. Hughes, and Christopher T. Lam
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0303 health sciences ,Fluorescence-lifetime imaging microscopy ,biology ,business.industry ,medicine.disease ,01 natural sciences ,Molecular biomarkers ,Hsp90 ,Atomic and Molecular Physics, and Optics ,010309 optics ,03 medical and health sciences ,Breast cancer ,0103 physical sciences ,Medical imaging ,medicine ,biology.protein ,Cancer research ,Molecular imaging ,business ,Molecular probe ,Ex vivo ,030304 developmental biology ,Biotechnology - Abstract
Overexpression of heat shock protein 90 (Hsp90) on the surface of breast cancer cells makes it an attractive molecular biomarker for breast cancer diagnosis. Before a ubiquitous diagnostic method can be established, an understanding of the systematic errors in Hsp90-based imaging is essential. In this study, we investigated three factors that may influence the sensitivity of ex vivo Hsp90 molecular imaging: time-dependent tissue viability, nonspecific diffusion of an Hsp90 specific probe (HS-27), and contact-based imaging. These three factors will be important considerations when designing any diagnostic imaging strategy based on fluorescence imaging of a molecular target on tissue samples.
- Published
- 2021
17. Rapid characterization of chemical constituents of Gansuibanxia decoction by UHPLC-FT-ICR-MS analysis
- Author
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Yunli Zhao, Yue Cui, Huanhuan Yang, Fuyu Di, Ting Liu, Roujia Wang, Fei Han, Jixue Jing, and Zhiguo Yu
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chemistry.chemical_classification ,Chromatography ,010405 organic chemistry ,Monoterpene ,010401 analytical chemistry ,Clinical Biochemistry ,Pharmaceutical Science ,Glycoside ,Decoction ,Traditional Chinese medicine ,01 natural sciences ,Mass Spectrometry ,0104 chemical sciences ,Analytical Chemistry ,Triterpenoid ,chemistry ,In vivo ,Ft icr ms ,Chemical constituents ,Drug Discovery ,Chromatography, High Pressure Liquid ,Spectroscopy ,Drugs, Chinese Herbal - Abstract
Gansuibanxia decoction (GSBXD) is one of the most famous traditional Chinese medicine (TCM). It is a herbal formula used for treating hydrops, such as cancerous ascites, pleural effusion, pericardial effusion, etc. However, the chemical constituents of GSBXD were still unclear. In this study, an UHPLC-FT-ICR-MS method was established and applied to the separation and characterization of the chemical constituents of GSBXD. A total of 62 components were chemically defined or tentatively identified, including diterpenoids, triterpenoids, flavonoids, monoterpene glycosides and alkaloids. The results is meaningful for a better understanding of the material basis of GSBXD and can be the basis for its further in vitro and in vivo studies.
- Published
- 2020
18. Application of hair analysis to document illegal 5-methoxy-N,N-dissopropyltrptamine (5-MeO-DiPT) use
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Yan Shi, Roujia Wang, Ping Xiang, and Zhiguo Yu
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Adult ,Male ,Substance-Related Disorders ,Formic acid ,Calibration curve ,Derivative ,Mass Spectrometry ,Designer Drugs ,Pathology and Forensic Medicine ,5-Methoxytryptamine ,Forensic Toxicology ,Young Adult ,chemistry.chemical_compound ,Limit of Detection ,Humans ,Acetonitrile ,Chromatography, High Pressure Liquid ,Detection limit ,Chromatography ,Molecular Structure ,Hair analysis ,Middle Aged ,Substance Abuse Detection ,Solvent ,chemistry ,Hallucinogens ,Female ,Law ,Ammonium acetate ,Hair - Abstract
5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DiPT) is a designer hallucinogen that is a synthetic tryptamine derivative. It is highly abused and is involved in criminal activities because of its psychotropic properties. Herein, we presented an UHPLC-MS/MS method allowing for the qualitative and quantitative determination of 5-MeO-DiPT in human hair. The hair was first decontaminated and then cut into pieces. Thirty milligrams of hair samples was pulverized below 4°C in the presence of 0.5mL deionized water containing 0.1% formic acid. After centrifuging twice, 5μL of supernatant was injected into the LC-MS/MS system. A T3 column (100mm×2.1mm, 1.8μm) was used, and mobile phases consisted of 20mmol/L ammonium acetate, 5% acetonitrile and 0.1% formic acid in water (solvent A) and acetonitrile (solvent B). The gradient elution was used at a flow rate of 0.3mL/min. The resulting calibration curve for 5-MeO-DiPT was y=281.50213x+0.00231 (R2=0.992), the limit of detection (LOD) was 0.05pg/mg, and the lower limit of quantification (LLOQ) was 0.1pg/mg. The accuracy was between 92.1% and 105.6%, and the intra- and interday precision, recovery and matrix effect were acceptable. The validated method was successfully used in 106 real cases, and the concentration of 5-MeO-DiPT in hair samples of these suspected users was 0.2-7532.5pg/mg. These cases present data to document illegal 5-MeO-DiPT use.
- Published
- 2019
19. Simultaneous determination of five bioactive components of Gancao in rat plasma by UHPLC-MS/MS and its application to comparative pharmacokinetic study of incompatible herb pair Gansui-Gancao and Gansuibanxia Decoction
- Author
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Ting Liu, Yue Cui, Peipei Yu, Qili Zhang, Ye Zhang, Roujia Wang, Xiaozhou Liu, Zhiguo Yu, and Yunli Zhao
- Subjects
Male ,Clinical Biochemistry ,Pharmaceutical Science ,Decoction ,Paeonia ,030226 pharmacology & pharmacy ,01 natural sciences ,Analytical Chemistry ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,Acetic acid ,Random Allocation ,0302 clinical medicine ,Pharmacokinetics ,Tandem Mass Spectrometry ,Drug Discovery ,Glycyrrhiza ,Animals ,Spectroscopy ,Chromatography, High Pressure Liquid ,Chromatography ,biology ,010401 analytical chemistry ,Selected reaction monitoring ,biology.organism_classification ,0104 chemical sciences ,Rats ,chemistry ,Pinellia ,Liquiritigenin ,Liquiritin ,Isoliquiritigenin ,Drugs, Chinese Herbal - Abstract
Incompatible herb pair Gansui-Gancao is recorded in "eighteen incompatible" medicaments in many monographs of TCM (Traditional Chinese Medicine) which means the two herbs can not be co-used in most cases. However, Gansuibanxia decoction composed of Gansui(Kansui), Banxia(Pinellia), Shaoyao(Peony) and Gancao(Liquorice) is a traditional Chinese formula which has been clinically employed for the treatment of cancerous ascites, pleural effusion, peritoneal effusion, etc. The purpose of the study was to investigate the pharmacokinetics of main bioactive components in Gancao to explore the reasons why Gansui-Gancao can be used in Gansuibanxia decoction. A simple, rapid and sensitive UHPLC-MS/MS method for simultaneous determination of liquiritigenin, isoliquiritigenin, liquiritin, glycyrrhetinic acid and glycyrrhizic acid of liquorice in rat plasma was developed and validated. After extraction from plasma, the analytes and internal standard were separated on a C18 column with the mobile phase consisting of 0.1% acetic acid containing 0.2 mM ammonium acetate in water and acetonitrile via gradient elution. The electrospary ionization source was adopted under the multiple reaction monitoring mode. The method was succesfully applied to a comparative pharmacokinetic study of main bioactive components of Gancao in rat plasma after oral administration of the extracts of Gancao (GC), Gansui-Gancao (GS-GC), Shaoyao-Gancao (SY-GC), Gansui-Shaoyao-Gancao (GS-SY-GC) and Gansuibanxia decoction (GSBXD), respectively. The pharmacokinetic parameters had significant differences (P 0.05) in different groups which showed that Gansui decreased the bioavailability of Gancao, while Shaoyao increased the bioavailability of Gancao. Hence, these may be the pharmacokinetic mechanism of incompatible herb pair Gansui-Gancao and the reasons why the herb pair can be used in Gansuibanxia decoction.
- Published
- 2018
20. Optimizing fluorescently-tethered Hsp90 inhibitor dose for maximal specific uptake by breast tumors
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Roujia Wang, Allison Hall, Timothy A.J. Haystead, Mary Scott Soo, Brian T. Crouch, Joy Duer, Nirmala Ramanujam, Jennifer Gallagher, and Philip F. Hughes
- Subjects
Fluorescence-lifetime imaging microscopy ,medicine.diagnostic_test ,business.industry ,Cancer ,medicine.disease ,Hsp90 inhibitor ,chemistry.chemical_compound ,Breast cancer ,chemistry ,Biopsy ,Cancer research ,medicine ,Molecular imaging ,Fluorescein isothiocyanate ,business ,Ex vivo - Abstract
Despite improvements in surgical resection, 20-40% of patients undergoing breast conserving surgery require at least one additional re-excision. Leveraging the unique surface expression of heat shock protein 90 (Hsp90), a chaperone protein involved in several key hallmarks of cancer, in breast cancer provides an exciting opportunity to identify residual disease during surgery. We developed a completely non-destructive strategy using HS-27, a fluorescently-tethered Hsp90 inhibitor, to assay surface Hsp90 expression on intact tissue specimens using a fluorescence microendoscope with a field of view of 750 μm and subcellular resolution of 4 μm. HS-27 consists of an FDA approved Hsp90 inhibitor tethered to fluorescein isothiocyanate (EX 488nm, EM 525nm). Here, we optimized ex vivo HS-27 administration in pre-clinical breast cancer models and validated our approach on 21 patients undergoing standard of care ultrasound guided core needle biopsy. HS-27 administration time was fixed at 1- minute to minimize imaging impact on clinical workflow. HS-27 and HS-217 (non-specific control) doses were modulated from 1 μM up to 100 μM to identify the dose maximizing the ratio of specific uptake (HS-27 fluorescence) to non-specific uptake (HS-217 fluorescence). The specificity ratio was maximized at 100 μM and was significantly greater than all other doses (p
- Published
- 2018
21. [Effects of non-physical factors on neck and shoulder pain and low back pain of adolescents]
- Author
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Yongxing, Zhang, Guoying, Deng, Sheng, Zhao, Qian, Zhou, Xiang, Gao, Hang, Wang, Zhiqing, Zhang, Yaping, Ju, Roujia, Wang, Yuyuan, Wang, and Qinghua, Zhao
- Subjects
China ,Logistic Models ,Neck Pain ,Adolescent ,Risk Factors ,Shoulder Pain ,Incidence ,Surveys and Questionnaires ,Body Weight ,Humans ,Sleep ,Students ,Low Back Pain - Abstract
To explore the incidence of self-reported neck/shoulder pain (NSP) and lower back pain (LBP) among Chinese adolescents in Shanghai and identify the influencing factors for the incidences of these musculoskeletal disorders.A total of 3 600 students were selected from 30 high schools randomly chosen from 237 regular full-time high schools registered in Shanghai. From each school, 40 students were selected from each of the tenth, eleventh and twelfth grades for a total of 120 students per school. The questionnaire involved questions pertaining to demographic information, learning environment and exercise habits of each student. The questionnaire also contained questions regarding the amount of weight carried by each student while commuting to and from school. And it was also used to collect specific information related with the occurrence of NSP and LBP. Logistic regression was performed to analyze the potential risk factors for NSP and LBP.Among 3 600 distributed questionnaires, a total of 2 842 valid questionnaires were returned. The results revealed that the incidences of NSP and LBP in the Chinese adolescent population were 41.1% and 32.8%, respectively. Both NSP and LBP were more common in girls than in boys. And 6.3% students reported at least one NSP- or LBP-induced absence from school. Chinese adolescents generally experienced a heavy academic burden (32.7% failing to achieve daily academic goals) and mental stress (16.3% suffering from insomnia); the problem of insufficient sleep was even more pronounced (52.0% falling asleep after midnight and 64.3% suffering insufficient sleep while only 31.9% feeling physically relaxed after awaking). The multivariate Logistic regression analysis suggested that gender, grade, academic burden, stress and sleep situation had a significant correlation with NSP and LBP in adolescents.The incidences of NSP and LBP are relatively high among adolescents in Shanghai. And several factors, including sedentary behaviors, personal exercise habits and backpack weight, influences the occurrences of NSP and LBP in youth.
- Published
- 2015
22. Optimizing fluorescently-tethered Hsp90 inhibitor dose for maximal specific uptake by breast tumors.
- Author
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Crouch, Brian T., Duer, Joy, Roujia Wang, Gallagher, Jennifer, Hall, Allison, Scott^Soo, Mary, Hughes, Philip, Haystead, Timothy A. J., and Ramanujam, Nirmala
- Published
- 2018
- Full Text
- View/download PDF
23. Pulmonary Infection Within 100 Days After Transplantation Impaired Platelet Recovery in Patients with Hematologic Malignancies: A Propensity-Score-Matched Analysis.
- Author
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Roujia Wang, Aijie Huang, Qi Chen, Libing Wang, Lei Gao, Huiying Qiu, Xiong Ni, Weiping Zhang, Jianmin Yang, Jianmin Wang, and Xiaoxia Hu
- Published
- 2019
- Full Text
- View/download PDF
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