36 results on '"Rouatbi M"'
Search Results
2. Genome-wide insights on gastrointestinal nematode resistance in autochthonous Tunisian sheep
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Ahbara, A. M., Rouatbi, M., Gharbi, M., Rekik, M., Haile, A., Rischkowsky, B., and Mwacharo, J. M.
- Published
- 2021
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3. Molecular Study of Dirofilaria immitis and Dirofilaria repens in Dogs from Tunisia
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Rjeibi, M. R., Rouatbi, M., Mabrouk, M., Tabib, I., Rekik, M., and Gharbi, M.
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- 2017
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4. Dynamics of infestation by Rhinoestrus usbekistanicus in donkeys (Equus asinus) in Tunisia
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ROUATBI, M., primary, JEBLI, H., additional, DHIBI, M., additional, JEBABLI, L., additional, and GHARBI, M., additional
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- 2020
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5. First detection and molecular identification of Sarcocystis spp. in small ruminants in North-West Tunisia
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Amairia, S., primary, Amdouni, Y., additional, Rouatbi, M., additional, Rjeibi, M. R., additional, Awadi, S., additional, and Gharbi, M., additional
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- 2017
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6. Leishmaniosis (Leishmania infantum infection) in dogs
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Mohamed Gharbi, Mhadhbi M, Rejeb A, Jaouadi K, Rouatbi M, and Ma, Darghouth
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Dogs ,Zoonoses ,Antiprotozoal Agents ,Animals ,Humans ,Leishmaniasis, Visceral ,Dog Diseases ,Leishmania infantum - Abstract
The authors present an overview of canine leishmaniosis due to Leishmania infantum. This protozoan is transmitted by sandflies and the disease is frequently characterised by chronic evolution. Cutaneous and visceral clinical signs appear as the infection progresses. Lymph node enlargement, emaciation and skin lesions are the main signs observed in the classical forms of the disease. Control is difficult since infected dogs remain carriers for years and may relapse at any time. The mass screening of infected animals and their treatment or euthanasia represent the best way to reduce the prevalence of this disease in endemic regions. Further research is needed to improve the efficiency of the vaccines available to protect dogs against infection. This disease is zoonotic; in humans, clinical cases are reported mainly in elderly people, the young and those whose immune systems have been compromised.
- Published
- 2015
7. Dynamics of infestation by Rhinoestrus usbekistanicus in donkeys (Equus asinus) in Tunisia.
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Rouatbi, M., Jebli, H., Dhibi, M., Jebabli, L., and Gharbi, M.
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- 2019
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8. Molecular Study of Dirofilaria immitis and Dirofilaria repens in Dogs from Tunisia
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Rjeibi, M. R., primary, Rouatbi, M., additional, Mabrouk, M., additional, Tabib, I., additional, Rekik, M., additional, and Gharbi, M., additional
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- 2016
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9. First detection and molecular identification of <italic>Sarcocystis</italic> spp. in small ruminants in North‐West Tunisia.
- Author
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Amairia, S., Amdouni, Y., Rouatbi, M., Rjeibi, M. R., Awadi, S., and Gharbi, M.
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SARCOCYSTIS ,ANIMAL diseases ,RUMINANTS ,ETIOLOGY of diseases ,DISEASE prevalence - Abstract
Summary: Sarcocystosis is a parasitic disease caused by varying
Sarcocystis species infecting humans and animals. It is commonly found in small ruminants causing pathogenic effects. This contributes to detrimental economic loss for local farmers and the local economy due this disease. Although the distribution ofSarcocystis can be found all over the world, the species infecting small ruminants in Tunisia is still unknown. Through this study, we aim to estimate the molecular prevalence of natural infection withSarcocystis spp. in sheep and goats using molecular identification. Also, phylogenetic analyses were used to identify the different species of this parasite infecting small ruminants in northern Tunisia for the first time. DNA was extracted from 198 and 121, sheep and goats meat samples, respectively. The molecular prevalence ofSarcocystis spp. in sheep and goats was 58.6% (116/198) and 50.4% (61/121), respectively. Compared to the Noire de Thibar and cross‐breeds, the Barbarine sheep had the highest infection prevalence (63.4%) (p =Sarcocystis tenella from sheep. For goats, the sequencing results showed that five positive PCR products belonged toSarcocystis capracanis species. [ABSTRACT FROM AUTHOR]- Published
- 2018
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10. Leishmaniosis (Leishmania infantum infection) in dogs
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GHARBI, M., primary, MHADHBI, M., additional, REJEB, A., additional, JAOUADI, K., additional, ROUATBI, M., additional, and DARGHOUTH, M.A., additional
- Published
- 2015
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11. Toxoplasma gondii infection and toxoplasmosis in North Africa: a review
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Rouatbi Mariem, Amairia Safa, Amdouni Yosra, Boussaadoun Mohamed Anis, Ayadi Ouarda, Al-Hosary Amira Adel Taha, Rekik Mourad, Ben Abdallah Rym, Aoun Karim, Darghouth Mohamed Aziz, Wieland Barbara, and Gharbi Mohamed
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Toxoplasma gondii ,North Africa ,Humans ,Animals ,Infectious and parasitic diseases ,RC109-216 - Abstract
Toxoplasmosis is an important zoonosis caused by an obligate intracellular parasitic protozoan, Toxoplasma gondii. The disease is distributed worldwide and can affect all warm-blooded vertebrates, including humans. The present review aimed to collect, compile and summarize the data on the prevalence of T. gondii infection in humans and animals in the five North African countries (Morocco, Algeria, Tunisia, Libya and Egypt). Published data from national and international databases were used. Distribution patterns and risk factors for T. gondii infection are discussed, focusing on biotic and abiotic factors. This review is a comprehensive epidemiological analysis of T. gondii infection in North Africa and will therefore be a useful tool for researchers. It can also be used to propose or enhance appropriate national toxoplasmosis control programs.
- Published
- 2019
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12. Autoimmune Thyroid Disorders Are More Prevalent in Patients with Celiac Disease: A Retrospective Case-Control Study.
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Dore MP, Fanciulli G, Rouatbi M, Mereu S, and Pes GM
- Abstract
Background. Among patients with celiac disease (CD), there is an increased incidence of autoimmune thyroid disorders (AITDs), with hypothyroidism being more frequent than hyperthyroidism. This retrospective case-control study aimed to explore the prevalence of TDs in a population of adult celiac patients from Northern Sardinia, a geographic area with a high prevalence of autoimmune disorders. Methods. Data were collected from consecutive patients with CD (cases) and without CD (controls) who were undergoing upper endoscopy and referred to a tertiary gastroenterology section of a teaching hospital (University of Sassari, Italy). Thyroid disorders were stratified as (i) autoimmune: including Hashimoto’s disease in euthyroidism or with hypofunction, and Graves’ disease; or (ii) non-autoimmune: thyroid nodules/goiter, iatrogenic thyroid hypo/hyperfunction, and thyroidectomy for any reason, including cancer. Results. Among a total of 8489 participants (females 5839, 64.7%) enrolled, there were 623 (7.3%) celiac patients and 7866 controls (92.7%). The overall frequency of TDs was 1177 (13.9%) and was higher (26.0%) in celiac patients than in controls (12.9%) (p < 0.001). The difference between AITDs (15.4% vs. 7.5%) and no-AITDs (2.7% vs. 1.1%) was statistically significant in CD patients compared with controls, respectively, and prevailed in the fifth and sixth decades. Hashimoto’s thyroiditis was more commonly associated with gland hypofunction. Odds ratios with their 95% confidence intervals (CIs) for the presence of AITDs were calculated, adjusting for sex, age, body mass index, smoking habits, occupation, and residence, and they were 2.387 (95% CI 1.857−3.068, p < 0.001) in CD patients, 5.855 (95% CI 4.434−7.731, p < 0.001) for female sex, and 1.012 (95% CI, 1.007−1.017, p < 0.001) for age. Conclusion. These results suggest the need for surveillance for TDs in patients with CD at onset and during follow-up.
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- 2022
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13. High Toxoplasma gondii frequency of infection in the genital tract of ewes previously positive for the parasite DNA in meat, in Sidi Bouzid, Tunisia.
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Rouatbi M, Amairia S, Samed S, Rekik M, Darghouth MA, Jeljli A, and Gharbi M
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- Animals, DNA, DNA, Protozoan genetics, Female, Genitalia, Meat, Prevalence, Sheep, Tunisia epidemiology, Parasites, Toxoplasma genetics, Toxoplasmosis, Animal epidemiology
- Abstract
The aim of the present study was to estimate the molecular prevalence of T. gondii along various segments of the genital tract of confirmed chronically infected ewes. Genital tracts were collected from 42 ewes; meat samples from the same ewes were previously confirmed positive for T. gondii DNA. The whole DNA was extracted from 4 parts of the genital tract (ovary, horns, body of the uterus and vagina). PCR was used to amplify a 114 base-pairs of T. gondii B1 gene. For all studied samples, 95.2 % had at least one infected genital part. Toxoplasma gondii infection was confirmed by sequencing 20 amplicons randomly chosen. The majority of infected animals has 4 T. gondii-infected genital anatomical parts. Principal component analysis (PCA) revealed a cluster of adult animals (>24 months) with positive PCR in the ovaries and the vagina and another cluster of Barbarine animals having positive PCR in the horns and body of the uterus. General linear model confirmed PCA results and showed a significant higher prevalence of T. gondii in the ovaries and vagina of older animals (p = 0.001 and p = 0.02, respectively) and a higher prevalence of T. gondii in the horns of the uterus of Barbarine animals (p = 0.03). Toxoplasma gondii seems to highly persist along the various segments of the ewe's genital tract but further investigations are necessary to link such prevalence with the pathological implications., (Copyright © 2021. Published by Elsevier B.V.)
- Published
- 2021
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14. Tick Infestation and Piroplasm Infection in Barbarine and Queue Fine de l'Ouest Autochthonous Sheep Breeds in Tunisia, North Africa.
- Author
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Khamassi Khbou M, Rouatbi M, Romdhane R, Sassi L, Jdidi M, Haile A, Rekik M, and Gharbi M
- Abstract
As ticks and tick-borne pathogens affect the productivity of livestock, searching for genetically resistant breeds to infestation by ticks may represent an alternative to the overuse of chemical drugs. The aim of this study was to assess if there is a difference in tick infestation among the main sheep breeds in Tunisia. The study was carried out between April 2018 and January 2020 in 17 small to middle-sized sheep flocks from 3 regions across Tunisia. Four hundred and thirty-nine ear-tagged ewes from Barbarine (n = 288, 65.6%) and Queue Fine de l'Ouest (QFO) (n = 151, 34.4%) breeds were examined and sampled each trimester. Ticks were identified to the species level, and piroplasms were detected using PCR that targets a common sequence ARNr18S to both Babesia and Theileria genera using catch-all primers. Totally, 707 adult ticks were collected from animals; 91.4% (646/707) of them were Rhipicephalus sanguineus s.l. Queue Fine de l'Ouest animals were markedly less infested by ticks, and no one of them was infected by piroplasms compared to the Barbarine breed. Indeed, during the first four seasons, 21 animals, all from the Barbarine breed, were detected positive for piroplasms. This is the first study in Tunisia about the low susceptibility of QFO ewes to infestation by ticks and to infection by piroplasms. The QFO sheep breed could be raised preferably at high-risk areas of tick occurrence and could be considered in concrete control strategies, including a breeding program.
- Published
- 2021
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15. Individual variability among autochthonous sheep in Northern Tunisia to infection by abomasum nematodes and Babesia/Theileria parasites.
- Author
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Rouatbi M, Romdhane R, Bouaicha F, Saddem R, Sassi L, Dhibi M, Rekik M, Haile A, Mwacharo JM, Rischkowsky B, Darghouth MA, and Gharbi M
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- Abomasum, Animals, Babesia isolation & purification, Babesiosis parasitology, Biological Variation, Individual, Female, Individuality, Male, Nematoda isolation & purification, Nematode Infections epidemiology, Nematode Infections parasitology, Sheep, Sheep Diseases parasitology, Sheep, Domestic, Theileria isolation & purification, Theileriasis parasitology, Tunisia epidemiology, Babesiosis epidemiology, Nematode Infections veterinary, Sheep Diseases epidemiology, Theileriasis epidemiology
- Abstract
In Tunisia, livestock plays an important role in the economy; unfortunately, Tunisian sheep population faces several health challenges. The aim of this trial was to study phenotypic variability of four local sheep breeds and strains to abomasum nematodes infection and to Babesia/Theileria parasites. Faeces, blood and abomasum contents were collected from 310 sheep slaughtered in eight commercial slaughterhouses across North Tunisia. Haematological and biochemical parameters were assessed. DNA was extracted and catch-all primers were used to detect both Theileria spp. and Babesia spp. DNA. Faecal egg counts (FEC) was quantitatively assessed using simple flotation technique followed by McMaster technique. Male and female worms were collected from all abomasum contents and counted under a stereomicroscope. The percentage of faeces samples positive for GIN's eggs was 30.82%. After worms' recovery, the infection prevalence was estimated to 75.90%. The overall infection prevalence by Babesia spp. and Theileria spp. was 4.21%. The dispersion of observations plots obtained by principal component analysis (PCA) showed two clusters of individuals. The first cluster contains animals having positive Babesia/Theileria PCR, presence of nematodes in the abomasum contents and relatively low total worm count (TWC < 500) expect one animal which was found bearing high TWC (>500). In this same group, with a suspected form of resistance, animals showed normal values of albumin and normal haematological parameters (red blood cell count [RBC], haemoglobin [Hb] and packed cell volume [PCV]). The second cluster represents all the other observations in which subgroups of animals were distinguished on the basis of their potential resistance to abomasum nematodes. Multiple correlations showed significant positive correlations between RBC/Hb, RBC/PCV, PCV/Hb and FEC/TWC. Significant negative correlations were observed between TWC/RBC and TWC/Hb. It is concluded that the phenotypic variability among local sheep breeds is essential for more advanced genetic and genomic studies., (© 2020 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.)
- Published
- 2020
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16. Increased number of large non-atretic follicles and co-dominance effects account for high litter sizes in Bonga sheep.
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Tera Dolebo A, Melesse A, Porcu C, Getachew T, Haile A, Rouatbi M, Abate Z, Zeleke M, Rischkowsky B, Mwacharo JM, and Rekik M
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- Animals, Estradiol blood, Estrus physiology, Female, Litter Size, Ovarian Follicle anatomy & histology, Ovarian Follicle physiology, Ovulation physiology, Sheep physiology
- Abstract
To understand the ovarian basis for prolificacy of Bonga sheep, a total of 31 ewes were selected based on litter size (LS) records and divided into two groups: High Prolificacy (HP) (n = 20) with LS ≥ 2 and Low Prolificacy (LP) (n = 11) with LS = 1. At a synchronized estrus, follicular dynamics were determined using transrectal ultrasonography. Plasma estradiol concentrations were also monitored. In total 27 ewes were observed in estrus being 9/11 LP (82%) and 18/20 HP (90%). On the day of estrus (day 0), the mean number of large follicles was higher (p < .05) in HP (1.78 ± 0.19) than in LP (1.0 ± 0.28) ewes. Prior to estrus, more (p < .05) medium follicles were visible for HP compared to LP ewes. Plasma estradiol concentrations were higher in HP compared to LP ewes (18.91 ± 0.41 vs. 14.51 ± 0.65 pg/ml; p < .05) and similarly was ovulation number (2.3 ± 0.15 vs. 1.28 ± 0. 14; p < .05). Higher ovulation rates and litter size in Bonga sheep are evidenced by the previous presence of more large follicles and the existence of co-dominance effects as most likely medium follicles are selected to ovulate., (© 2020 Japanese Society of Animal Science.)
- Published
- 2020
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17. Detection of Toxoplasma gondii infection in semen of rams used for natural mating in commercial sheep farms in Tunisia.
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Rouatbi M, Amairia S, Lahmer M, Lassoued N, Rekik M, Wieland B, Mwacharo JM, and Gharbi M
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- Animals, Breeding, Enzyme-Linked Immunosorbent Assay veterinary, Male, Prevalence, Seroepidemiologic Studies, Sheep, Sheep Diseases parasitology, Toxoplasmosis, Animal parasitology, Tunisia epidemiology, Semen parasitology, Sheep Diseases epidemiology, Toxoplasma isolation & purification, Toxoplasmosis, Animal epidemiology
- Abstract
Toxoplasmosis, a parasitic infection, disrupts sexual functions resulting in decline in reproductive and economic performance in sheep. Lacking discernible symptoms, toxoplasmosis is difficult to detect and diagnose in infected animals. Here, to estimate the prevalence of natural infection by Toxoplasma gondii, we used PCR to amplify and detect T. gondii DNA in semen from 92 rams of three breeds from four regions in Tunisia and seroprevalence was determined using a commercial ELISA kit. From the PCR amplifications, 51.09 ± 10.21% of the rams were tested positive for T. gondii with an overall seroprevalence of 39.13 ± 9.97%. Risk factors including ram location and number of accomplished mating seasons significantly (p < .05) affected the sero- and molecular prevalence of T. gondii in semen but, there was a fair concordance between sero- and molecular prevalence (Kappa = 0.33). Sequences of T. gondii from five positive samples were 100% identical (same haplotype). Comparison of these sequences with those archived at the GenBank showed a sequence similarity range between 95 and 100%. The haplotype defining the five Tunisian sequences was similar to the one observed in chicken, cats, European pole cat and humans from Brazil, St Kitts and Nevis, Great Britain and Tunisia, respectively. This indicates its wide geographic distribution and non-species specificity. Our findings suggest a high prevalence of toxoplasmosis in Tunisian matting rams; further studies concerning its venereal transmission capacity are needed prior to recommending a systematic screening of T. gondii DNA in rams' semen used for both natural breeding and artificial insemination., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
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18. Dynamics of infestation by Rhinoestrus usbekistanicus in donkeys (Equus asinus) in Tunisia.
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Rouatbi M, Jebli H, Dhibi M, Jebabli L, and Gharbi M
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- Abattoirs, Animals, Larva, Nose parasitology, Tunisia, Diptera, Equidae parasitology, Myiasis veterinary
- Abstract
Examination of a total of 125 slaughtered donkeys in Tunisian slaughterhouses revealed the presence of 1,508 larvae of Rhinoestrus usbekistanicus in their nasal cavities, with an overall infestation prevalence of 52.8%. The highest overall intensity (number of larvae per infested donkey) and abundance (number of larvae per donkey examined) of infestation were reported in winter (35.6 and 12.1, respectively). Larvae 1 (L1) were the most frequent stage and reached a peak prevalence of 100% during November. The number of larvae 2 (L2) and 3 (L3) was low and the maximum was recorded during March and April. Post-mortem observations showed that infestation intensity increases with age. The ethmoid bone was the most frequently infected portion of the nasal cavity (83.75%). This is the first study on nasal bots in donkeys in Tunisia.
- Published
- 2019
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19. Neospora caninum Natural Infection in Tunisian Rams: Serological Study and Molecular Identification of Infection in Semen.
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Amdouni Y, Rouatbi M, Lassoued N, Rekik M, and Gharbi M
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- Animals, Antibodies, Protozoan blood, Cattle, Cattle Diseases parasitology, Coccidiosis epidemiology, DNA, Intergenic genetics, Enzyme-Linked Immunosorbent Assay, Farms, Immunoglobulin G blood, Male, Neospora genetics, Phylogeny, Prevalence, Seroepidemiologic Studies, Tunisia epidemiology, Cattle Diseases epidemiology, Coccidiosis veterinary, Neospora isolation & purification, Semen parasitology
- Abstract
Purpose: The aim of this study was to estimate the seroprevalence and molecular prevalence and perform a molecular identification of Neospora caninum in semen of Tunisian rams., Methods: A total of 92 blood samples were collected from four farms located in four Tunisian governorates (Jendouba, Kairouan, Zaghouan and Ben Arous) and samples were screened with a commercial ELISA kit for N. caninum antibodies. For the same rams, semen samples were collected and tested for the presence of N. caninum ITS1 gene using PCR. Five amplicons were randomly selected for sequencing. A phylogenetic tree was constructed to compare the partial sequences of the ITS1 gene with sequences deposited in GenBank., Results: The seroprevalence of N. caninum infection was 25% (23/92) and PCR revealed that the molecular infection prevalence in semen was 11.95% (11/92). Kappa test showed an average agreement between seroprevalence and parasite prevalence in semen (κ = 0.44). The highest molecular prevalence was for rams that accomplished more than two mating seasons (21.0 ± 12.1%) compared to those performed less than two mating seasons and yearling individuals (4.0 ± 5.5%) (P = 0.01). There were no differences in N. caninum molecular prevalence according to either breed or locality. Comparison of the partial sequences of the ITS1 gene revealed 99-100% similarity with those deposited in GenBank., Conclusion: To the best of our knowledge, this is the first detection and molecular identification of N. caninum in semen from rams in North Africa. Our findings indicate that N. caninum infection rate was high in rams.
- Published
- 2019
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20. First detection and molecular identification of Sarcocystis spp. in small ruminants in North-West Tunisia.
- Author
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Amairia S, Amdouni Y, Rouatbi M, Rjeibi MR, Awadi S, and Gharbi M
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- Animals, Base Sequence, Goat Diseases diagnosis, Goat Diseases epidemiology, Goats, Humans, Molecular Diagnostic Techniques, Phylogeny, Polymerase Chain Reaction veterinary, Prevalence, Sarcocystis genetics, Sarcocystosis diagnosis, Sarcocystosis epidemiology, Sheep, Sheep Diseases diagnosis, Sheep Diseases epidemiology, Tunisia epidemiology, Goat Diseases parasitology, Sarcocystis isolation & purification, Sarcocystosis veterinary, Sheep Diseases parasitology
- Abstract
Sarcocystosis is a parasitic disease caused by varying Sarcocystis species infecting humans and animals. It is commonly found in small ruminants causing pathogenic effects. This contributes to detrimental economic loss for local farmers and the local economy due this disease. Although the distribution of Sarcocystis can be found all over the world, the species infecting small ruminants in Tunisia is still unknown. Through this study, we aim to estimate the molecular prevalence of natural infection with Sarcocystis spp. in sheep and goats using molecular identification. Also, phylogenetic analyses were used to identify the different species of this parasite infecting small ruminants in northern Tunisia for the first time. DNA was extracted from 198 and 121, sheep and goats meat samples, respectively. The molecular prevalence of Sarcocystis spp. in sheep and goats was 58.6% (116/198) and 50.4% (61/121), respectively. Compared to the Noire de Thibar and cross-breeds, the Barbarine sheep had the highest infection prevalence (63.4%) (p = .004). Five of the 116 positive samples were sequenced identifying Sarcocystis tenella from sheep. For goats, the sequencing results showed that five positive PCR products belonged to Sarcocystis capracanis species., (© 2017 Blackwell Verlag GmbH.)
- Published
- 2018
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21. Molecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia.
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Sallemi S, Rjeibi MR, Rouatbi M, Amairia S, Ben Said M, Khamassi Khbou M, and Gharbi M
- Abstract
The present study aimed to estimate the molecular prevalence of Theileria annulata and Trypanosoma evansi infection in cattle in Northern Tunisia. A total number of 96 cattle from five farms were evaluated. T. annulata and T. evansi prevalences were 61% [56/66] and 10% [7/13], respectively, at a confidence interval (CI) of 95%, while co-infection was present in 6% [4/8] of the tested animals at a CI of 95%. There was a significant correlation between age and the prevalence of T. annulata infection, whereas, there was no significant association shown with the age of cattle and T. evansi infection. Sequence and phylogenetic analyses showed that the T. annulata Tams1 gene and T. evansi ITS1 rDNA gene were highly conserved with 97.1-100% and 98.3-100% sequence identity, respectively.
- Published
- 2017
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22. Molecular detection of Toxoplasma gondii infection in slaughtered ruminants (sheep, goats and cattle) in Northwest Tunisia.
- Author
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Amdouni Y, Rjeibi MR, Rouatbi M, Amairia S, Awadi S, and Gharbi M
- Subjects
- Animals, Cattle, Cattle Diseases epidemiology, Cattle Diseases parasitology, DNA, Protozoan, Goat Diseases epidemiology, Goat Diseases parasitology, Goats, Polymerase Chain Reaction veterinary, Prevalence, Sequence Analysis, DNA, Sheep, Sheep Diseases epidemiology, Sheep Diseases parasitology, Toxoplasma genetics, Tunisia epidemiology, Meat parasitology, Toxoplasma isolation & purification, Toxoplasmosis, Animal epidemiology
- Abstract
The present study aimed to estimate the molecular prevalence of T. gondii infection in meat from slaughtered sheep, goats and cattle in Northwest Tunisia (Béja district). PCRs were performed on genomic DNA extracted from 420 meat samples (150 ewes, 120 goats and 150 cows). The overall molecular prevalence of T. gondii in sheep, goats and cattle were 33.3 (50/150), 32.5 (39/120) and 19.3% (29/150), respectively. Toxoplasma gondii molecular prevalences in the three meat ruminant species were significantly higher in adults compared to young animals (p<0.001). The infection prevalence differed significantly within localities in sheep (p<0.001), goats (p<0.001) and cattle (p=0.019). These results provide important information about exposure of humans to T. gondii through the consumption of raw or undercooked ruminants' meat. Extension programmes should be implemented to decrease the risk of infection related to sheep, goats and cattle meat manipulation and raw or undercooked meat consumption., (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Published
- 2017
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23. FIRST REPORT OF TRYPANOSOMA EVANSI INFECTION (SURRA) IN A PUMA (FELIS CONCOLOR) OF LAHORE ZOO, PAKISTAN.
- Author
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Rashid I, Akbar H, Gharbi M, Riaz F, Islam S, Saleem MB, Shahzad S, Shehzad W, Rouatbi M, and Ashraf K
- Subjects
- Animals, Animals, Zoo, Antiprotozoal Agents therapeutic use, Diminazene analogs & derivatives, Diminazene therapeutic use, Fatal Outcome, Male, Trypanosomiasis drug therapy, Trypanosomiasis pathology, Puma, Trypanosoma classification, Trypanosomiasis veterinary
- Abstract
The blood protozoan Trypanosoma evansi, which is transmitted by biting flies, is frequently neglected due to subclinical infections. This report describes a case of trypanosomiasis due to T. evansi in a 9-yr-old male puma (Felis concolor) housed at the Lahore Zoo in Pakistan. Early in January 2015, this male puma presented with chronic lethargy, weight loss, incoordination, hyperthermia, anorexia, sunken eyes, and unthriftiness. Microscopic examination of Giemsa-stained blood smears showed numerous Trypanosoma parasites. The puma was treated with diminazene aceturate subcutaneously twice. A few days later, a blood smear examination showed absence of trypanosomes. Five months later the cat presented with acute epistaxis and died. Postmortem examination showed emaciation, pale liver and kidneys, and hemorrhages on the spleen. Examination of a blood smear taken at the time of death showed numerous Trypanosoma parasites. PCR testing confirmed the presence of Trypanosoma DNA. DNA sequencing of two amplicons confirmed the presence of Trypanosoma in the blood smears with a 98-99% identity with the previously identified GenBank sequences. A phylogenetic tree was then constructed. Further studies are needed to improve our knowledge about the epidemiology and pathogenesis of T. evansi infection in wild animal species.
- Published
- 2017
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24. Molecular detection and phylogenetic analyses of Toxoplasma gondii from naturally infected sheep in Northern and Central Tunisia.
- Author
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Rouatbi M, Amdouni Y, Amairia S, Rjeibi MR, Sammoudi S, Rekik M, and Gharbi M
- Abstract
Toxoplasmosis is a worldwide zoonosis caused by the parasitic protozoan Toxoplasma gondii . It can infect all warm-blooded vertebrate species and causes abortions and birth defects in pregnant women and pregnant ewes. The objective of this study was to estimate the prevalence of infection with T. gondii in sheep meat in the region of Sidi Bouzid (central Tunisia) and Beja (northern Tunisia), the realization of a descriptive study of risk factors and the phylogenetic analyses of T. gondii . Neck muscle samples were obtained from 174 ewes and ewe lamb slaughtered in Sidi Bouzid and 150 lambs slaughtered in Beja. DNA was extracted from the samples using the Wizard
® genomic DNA purification kit. A nested PCR using two pairs of primers (NN 1 and NN2, Tg-NP1 and Tg-NP2) were used to detect infection with T. gondii , which was then confirmed by sequencing. Eight T. gondii amplicons were sequenced (accession number KT896498) and deposited in GenBank. The T. gondii amplicons showed 97-100% identities with GenBank sequences. A phylogenetic tree was then constructed. The nested PCR detected T. gondii DNA in 31% of animals tested in Sidi Bouzid and 32% of lambs tested in Beja. No significant difference in the prevalence of T. gondii infection was established between the two tested regions. In both regions, no significant variation of the infection depending on age, breed and locality was found.- Published
- 2016
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25. First molecular detection and characterization of Sarcocystis species in slaughtered cattle in North-West Tunisia.
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Amairia S, Amdouni Y, Rjeibi MR, Rouatbi M, Awadi S, and Gharbi M
- Subjects
- Animals, Cattle Diseases epidemiology, Cattle Diseases parasitology, Meat, Polymerase Chain Reaction veterinary, Polymorphism, Restriction Fragment Length, Prevalence, Sarcocystis classification, Sarcocystis genetics, Tunisia epidemiology, Cattle parasitology, Red Meat parasitology, Sarcocystis isolation & purification, Sarcocystosis epidemiology, Sarcocystosis veterinary
- Abstract
Sarcocystis spp. is one of the most prevalent foodborne parasites infecting both animals and humans. Consumption of raw or undercooked infected meat is a risk factor of human intestinal sarcocystosis. The present study aimed to estimate the prevalence of Sarcocystis species infecting slaughtered Tunisian cattle in North-West Tunisia (Béja governorate). DNA was extracted from 150 beef meat samples and a PCR-restriction fragment length polymorphism was used for identification. The overall infection prevalence of Sarcocystis spp. was 38% (57/150). Two species were identified, namely S. hominis (25%; 39/150) and S. cruzi (12%; 18/150). For both species, the highest prevalence was in Thibar locality (52.9 and 17.6% for S. hominis and S. cruzi, respectively). The molecular prevalence of S. cruzi was significantly higher in animals aged between two and eight years (19.2%; 10/52). This is the first molecular identification of Sarcocystis species in Tunisian cattle. Further studies in both human and animal Tunisian populations are needed to rank this parasitic disease among others., (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
26. Molecular prevalence and genetic characterization of piroplasms in dogs from Tunisia.
- Author
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Rjeibi MR, Amairia S, Rouatbi M, Ben Salem F, Mabrouk M, and Gharbi M
- Subjects
- Animals, Babesia classification, Babesia genetics, Babesiosis parasitology, Cluster Analysis, DNA, Protozoan chemistry, DNA, Protozoan genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Dog Diseases parasitology, Dogs, Molecular Epidemiology, Phylogeny, Polymerase Chain Reaction, Prevalence, Protozoan Proteins genetics, RNA, Ribosomal, 18S genetics, Seasons, Sequence Analysis, DNA, Theileria classification, Theileria genetics, Tunisia epidemiology, Babesia isolation & purification, Babesiosis epidemiology, Dog Diseases epidemiology, Theileria isolation & purification
- Abstract
In this study, the prevalence of piroplasms in dogs was assessed using polymerase chain reaction (PCR) to identify Babesia and Theileria species in 200 dogs from Northern and Central Tunisia between spring and autumn 2014. The overall molecular prevalence for piroplasms was 14·5% ± 0·05 (29/200); PCR detected 2 species, namely Babesia vogeli and Theileria annulata with an overall prevalence of 12·5 ± 0·04 and 2% ± 0·02, respectively. No differences in the molecular prevalences of B. vogeli were revealed for age and sex (P > 0·05). The molecular prevalence of B. vogeli was significantly higher in central Tunisia (26·5% ± 0·01) compared with the North (9·6% ± 0·04) (P 0·05). Comparison of the partial sequences of 18S rRNA and Tams 1 genes confirmed the presence of 2 novel B. vogeli and T. annulata genotypes. This is the first molecular detection of T. annulata and genetic characterization of dogs' piroplasms in Tunisia. Further studies are needed to better assess the epidemiological feature of piroplasms infection in North Africa.
- Published
- 2016
- Full Text
- View/download PDF
27. Effect of the infection with the nematode Haemonchus contortus (Strongylida: Trichostrongylidae) on the haematological, biochemical, clinical and reproductive traits in rams.
- Author
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Rouatbi M, Gharbi M, Rjeibi MR, Ben Salem I, Akkari H, Lassoued N, and Rekik M
- Subjects
- Animals, Body Weight, Case-Control Studies, Haemonchiasis parasitology, Host-Parasite Interactions, Male, Reproduction, Semen physiology, Sheep, Sheep Diseases blood, Sheep Diseases pathology, Testosterone blood, Haemonchiasis veterinary, Haemonchus pathogenicity, Sheep Diseases parasitology
- Abstract
This study aimed to investigate the effect of Haemonchus contortus infection on rams' haematological, biochemical and clinical parameters and reproductive performances. A total number of 12 Barbarine rams (control and infected) were included in the experiment. The infected group received 30 000 H. contortus third-stage larvae orally. Each ram's ejaculate was immediately evaluated for volume, sperm cell concentration and mortality rate. At the end of the experiment (day 82 post-infection), which lasted 89 days, serial blood samples were collected in order to assess plasma testosterone and luteinising hormone (LH) concentrations. There was an effect of time, infection and their interaction on haematological parameters (p < 0.001). In infected rams, haematocrit, red blood cell count and haemoglobin started to decrease from 21 days post-infection. There was an effect of time and infection for albumin. For total protein, only infection had a statistically significant effect. For glucose, only time had a statistically significant effect. Concentrations were significantly lower in infected rams compared to control animals. A significant effect of infection and time on sperm concentrations and sperm mortality was observed. The effect of infection appears in time for sperm concentrations at days 69 and 76 post-infection. Sperm mortality rate was significantly higher in infected animals at day 46 post-infection when compared to control group (p < 0.05). Finally, plasma testosterone traits (average concentration, cumulated levels during the sampling period and pulse frequency) were depressed in infected rams when compared to control counterparts; none of these endocrine traits were affected for plasma LH.
- Published
- 2016
- Full Text
- View/download PDF
28. Molecular prevalence of Toxoplasma gondii DNA in goats' milk and seroprevalence in Northwest Tunisia.
- Author
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Amairia S, Rouatbi M, Rjeibi MR, Nouasri H, Sassi L, Mhadhbi M, and Gharbi M
- Abstract
Toxoplasmosis is a worldwide zoonosis with high impact on human and animal health. Consumption of unpasteurized milk is a risk factor of human toxoplasmosis. The aim of this study was to estimate the seroprevalence and molecular prevalence of T. gondii in goats' milk in Northwest of Tunisia (Jendouba Governorate). A total number of 77 blood samples were collected from six herds were screened with a commercial ELISA kit for T. gondii antibodies. For the same goats' samples, a nested PCR was performed to detect T. gondii DNA in milk. The seroprevalence of T. gondii infection was 31.2% (±0.05) while the molecular prevalence of this parasite in milk was estimated to 7.8% (±0.03). A very low value of kappa showed that there is not agreement between seroprevalence and parasite prevalence in milk. These results suggest that the consumption of raw milk from naturally infected goats is a potential source of human infection. An extension programme should be implemented to decrease related to goats' raw milk consumption.
- Published
- 2016
- Full Text
- View/download PDF
29. Infestation of the spur-thighed tortoise (Testudo graeca) by Hyalomma aegyptium in Tunisia.
- Author
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Gharbi M, Rjeibi MR, Rouatbi M, Mabrouk M, Mhadhbi M, Amairia S, Amdouni Y, and Boussaadoun MA
- Subjects
- Animals, Female, Male, Tick Infestations epidemiology, Tunisia epidemiology, Ixodidae classification, Tick Infestations veterinary, Turtles parasitology
- Abstract
We examined 210 spur-thighed tortoises (Testudo graeca) for the presence of ticks in Tunisia during May 2014. A total number of 602 adult ticks were collected and identified leading to the estimation of parasitological indicators. All the ticks belonged to a single species: Hyalomma aegyptium. The mean infestation prevalence was 66.2%, mean overall infestation intensity and abundance were 4.33 and 2.86 ticks/tortoise respectively. Our survey showed that tortoises were significantly more infested by male ticks than females (p<0.001). The ticks were mainly present in the posterior limbs compared to other body regions (p<0.05). There was no significance variation of length and weight of tortoises according to sex (p<0.05). There was a significant correlation between the tortoises' size (length and weight) and tick infestation. This study showed high tick burdens of spur-thighed tortoises in Tunisia; further investigations are needed to determine exactly the role of this tick species in the transmission of different zoonotic pathogens., (Copyright © 2015 Elsevier GmbH. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
30. Long-pulsed Nd:YAG laser in the treatment of facial hypertrichosis during topical minoxidil therapy.
- Author
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Benmously Mlika R, Ben Hamida M, Hammami H, Dorbani Ben Thabet I, Rouatbi M, and Mokhtar I
- Subjects
- Administration, Topical, Alopecia Areata drug therapy, Child, Female, Humans, Hypertrichosis chemically induced, Minoxidil administration & dosage, Minoxidil adverse effects, Vasodilator Agents administration & dosage, Vasodilator Agents adverse effects, Hair Removal instrumentation, Hypertrichosis radiotherapy, Lasers, Solid-State, Low-Level Light Therapy
- Abstract
Hypertrichosis is a well-recognized adverse effect of therapy with either oral or topical minoxidil. We report a case of fronto-temporal hypertrichosis occurring in an 8-year-old girl treated for patchy alopecia areata of the frontal area of the scalp with 2% minoxidil solution. After failure of 5-months minoxidil-discontinuation, hair removal with Nd:YAG laser (1064 nm line) (Smartepil II, Deka) was tested leading to complete resolution within 2 sessions.
- Published
- 2013
- Full Text
- View/download PDF
31. Insect bite-like reaction associated with the relapse of non-Hodgkin B cell-lymphoma.
- Author
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Benmously R, Hammami H, Rouatbi M, Debbiche A, Souissi A, Mokhtar I, and Fenniche S
- Subjects
- Aged, Female, Humans, Insect Bites and Stings complications, Insect Bites and Stings immunology, Lymphoma, B-Cell immunology, Recurrence, Insect Bites and Stings pathology, Lymphoma, B-Cell pathology
- Published
- 2009
- Full Text
- View/download PDF
32. [Focal dermal hypoplasia].
- Author
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Rouatbi M, Zeglaoui F, and Ben Osman A
- Subjects
- Adolescent, Female, Humans, Focal Dermal Hypoplasia diagnosis
- Published
- 1997
33. [Bolus injection of corticosteroids in pemphigus. Report of four cases].
- Author
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Haouet H, Rouatbi M, and Ben Osman A
- Subjects
- Adult, Aged, Female, Humans, Injections, Intravenous, Male, Middle Aged, Adrenal Cortex Hormones administration & dosage, Pemphigus drug therapy
- Published
- 1996
34. [Analytical study of 13 cases of cutaneous lymphoma].
- Author
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Kort R, Mokni M, Rouatbi M, Azaiz MI, Kchir N, Haouet S, Boubaker S, Zitouna M, and Ben Osman A
- Subjects
- Antineoplastic Combined Chemotherapy Protocols therapeutic use, Biopsy, Cause of Death, Disease Progression, Female, Follow-Up Studies, Humans, Immunohistochemistry, Lymph Nodes pathology, Lymphoma, B-Cell drug therapy, Lymphoma, B-Cell radiotherapy, Lymphoma, T-Cell drug therapy, Lymphoma, T-Cell, Cutaneous drug therapy, Lymphoma, T-Cell, Cutaneous radiotherapy, Male, Middle Aged, Mycosis Fungoides pathology, Remission Induction, Skin pathology, Skin Neoplasms drug therapy, Skin Neoplasms radiotherapy, Lymphoma, B-Cell pathology, Lymphoma, T-Cell pathology, Lymphoma, T-Cell, Cutaneous pathology, Skin Neoplasms pathology
- Published
- 1995
35. [Psychosomatic approach in a self-induced dermatitis].
- Author
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Souissi R, Brour S, Rouatbi M, and Doss N
- Subjects
- Adult, Dermatitis diagnosis, Dermatitis therapy, Humans, Male, Psychophysiologic Disorders diagnosis, Psychophysiologic Disorders therapy, Dermatitis etiology, Diabetes Mellitus, Type 1 complications, Psychophysiologic Disorders etiology
- Published
- 1992
36. [Clinical case: neonatal cytosteatonecrosis. Apropos of a case].
- Author
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Souissi R, Kourda M, and Rouatbi M
- Subjects
- Cytoplasm ultrastructure, Female, Humans, Infant, Newborn, Skin pathology, Fat Necrosis pathology
- Published
- 1990
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