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Objective Minimally invasive procedures have been developed to reduce surgical trauma after cardiac surgery. Clinical recovery is the main focus of most research. Still, patient-centred outcomes, such as the quality of life, can provide a more comprehensive understanding of the impact of the surgery on the patient’s life. This systematic review aims to deliver a detailed summary of all available research investigating the quality of recovery, assessed with quality of life instruments, in adults undergoing minimally invasive cardiac surgery. Methods All randomised trials, cohort studies, and cross-sectional studies assessing the quality of recovery in patients undergoing minimally invasive cardiac surgery compared to conventional cardiac surgery within the last 20 years were included, and a summary was prepared. Results The randomised trial observed an overall improved quality of life after both minimally invasive and conventional surgery. The quality of life improvement in the minimally invasive group showed a faster course and evolved to a higher level than the conventional surgery group. These findings align with the results of prospective cohort studies. In the cross-sectional studies, no significant difference in the quality of life was seen except for one that observed a significantly higher quality of life in the minimally invasive group. Conclusions This systematic review indicates that patients may benefit from minimally invasive and conventional cardiac surgery, but patients undergoing minimally invasive cardiac surgery may recover sooner and to a greater extent. However, no firm conclusion could be drawn due to the limited available studies. Therefore, randomised controlled trials are needed.

Strain sensing seams have been developed by integrating conductive sewing threads in different types of seam designs on a fabric typical for sports clothing using sewing technology. The aim was to obtain a simply integrated stitch-based sensor that can be applied on sports clothing to monitor the movements of the upper body parts of the user during exercising. Stitch types 304; 406; 602 and 605 were produced. The seams were made on a knitted fabric composed of 80% polyamide 6.6 and 20% elastane. The seams underwent stretch cycling for 10 cycles and up to 44 cycles following EN ISO 14704-1:2005 (modified), using an INSTRON tensile tester machine. The changes in the resistance of the seams with time were recorded simultaneously using Agilent meter U1273A. Sensing functionality among which is sensor gauge factor (GF), stability, drift, and reproducibility were evaluated on the promising sensor seams. The type of base fabric used, stitch type, stitch formation process (friction and dynamic forces during sewing), integrated EC thread length, and positioning of thread(s) in the fabric have a significant influence on the performance of the seams. Sensor seam 406-001comprising 2 EC yarns (Madeira HC12) and Sensor seam 304-010 comprising 1 EC yarn (Madeira HC40) turned out to be very promising and others shall be improved (sensor 602-006 with Madeira HC 40 and sensor 605-002 with a Muriel yarn).

We assess the performance of mRNA capture sequencing to identify fusion transcripts in FFPE tissue of different sarcoma types, followed by RT-qPCR confirmation. To validate our workflow, six positive control tumors with a specific chromosomal rearrangement were analyzed using the TruSight RNA Pan-Cancer Panel. Fusion transcript calling by FusionCatcher confirmed these aberrations and enabled the identification of both fusion gene partners and breakpoints. Next, whole-transcriptome TruSeq RNA Exome sequencing was applied to 17 fusion gene-negative alveolar rhabdomyosarcoma (ARMS) or undifferentiated round cell sarcoma (URCS) tumors, for whom fluorescence in situ hybridization (FISH) did not identify the classical pathognomonic rearrangements. For six patients, a pathognomonic fusion transcript was readily detected, i.e., PAX3-FOXO1 in two ARMS patients, and EWSR1-FLI1, EWSR1-ERG, or EWSR1-NFATC2 in four URCS patients. For the 11 remaining patients, 11 newly identified fusion transcripts were confirmed by RT-qPCR, including COPS3-TOM1L2, NCOA1-DTNB, WWTR1-LINC01986, PLAA-MOB3B, AP1B1-CHEK2, and BRD4-LEUTX fusion transcripts in ARMS patients. Additionally, recurrently detected secondary fusion transcripts in patients diagnosed with EWSR1-NFATC2-positive sarcoma were confirmed (COPS4-TBC1D9, PICALM-SYTL2, SMG6-VPS53, and UBE2F-ALS2). In conclusion, this study shows that mRNA capture sequencing enhances the detection rate of pathognomonic fusions and enables the identification of novel and secondary fusion transcripts in sarcomas.

Lubrication is vital to improve performance, efficiency and durability in transmissions; it serves to minimize wear, noise, vibration and friction. The proper functioning of lubricated transmissions relies on interactions amongst a wide range of physical phenomena (contact dynamics, fluid-structure interaction and heat transfer) operating at different spatial and temporal scales. A lubricated transmission model is proposed in this work to obtain accurate information regarding all physical domains and the coupling thereof to quantify performance, efficiency and durability. The model consists of a thermo-elastically coupled flexible multibody model for the gear pair and a Thermo-Elasto-Hydrodynamic Lubrication (TEHL) model for the lubricant, both based on first principle modelling to ensure high fidelity. ispartof: Proceedings of ECCOMAS 2022 vol:Computational Solid Mechanics, 2022 ispartof: ECCOMAS Congress 2022 - 8th European Congress on Computational Methods in Applied Sciences and Engineering location:Oslo, Norway date:5 Jun - 9 Jun 2022 status: published

ispartof: Contemporanea vol:43 issue:4 status: Published online

Copy number alterations (CNAs) have increasingly become part of the diagnostic algorithm of glial tumors. Alterations such as homozygous deletion of CDKN2A/B, 7 +/ 10 - chromosome copy number changes or EGFR amplification are predictive of a poor prognosis. The codeletion of chromosome arms 1p and 19q, typically associated with oligodendroglioma, implies a more favorable prognosis. Detection of this codeletion by the current diagnostic standard, being fluorescence in situ hybridization (FISH), is sometimes however subject to technical and interpretation problems. In this study, we evaluated CNA detection by shallow whole-genome sequencing (sWGS) as an inexpensive, complementary molecular technique. A cohort of 36 glioma tissue samples, enriched with "difficult" and "ambiguous" cases, was analyzed by sWGS. sWGS results were compared with FISH assays of chromosomes 1p and 19q. In addition, CNAs relevant to glioblastoma diagnosis were explored. In 4/36 samples, EGFR (7p11.2) amplifications and homozygous loss of CDKN2A/B were identified by sWGS. Six out of 8 IDH-wild-type glioblastomas demonstrated a prognostic chromosome 7/chromosome 10 signature. In 11/36 samples, local interstitial and terminal 1p/19q alterations were detected by sWGS, implying that FISH's targeted nature might promote false arm-level extrapolations. In this cohort, differences in overall survival between patients with and without codeletion were better pronounced by the sequencing-based distinction (likelihood ratio of 7.48) in comparison to FISH groupings (likelihood ratio of 0.97 at diagnosis and 1.79 +/- 0.62 at reobservation), suggesting sWGS is more accurate than FISH. We recognized adverse effects of tissue block age on FISH signals. In addition, we show how sWGS reveals relevant aberrations beyond the 1p/19q state, such as EGFR amplification, combined gain of chromosome 7 and loss of chromosome 10, and homozygous loss of CDKN2A/B. The findings presented by this study might stimulate implementation of sWGS as a complementary, easy to apply technique for copy number detection.