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Introduction: Diabetes mellitus (DM) comorbidity in drug-resistant (DR) pulmonary tuberculosis (TB) patients can be associated with the treatment outcome. In DR-TB patients with DM, the immune system is impaired, which will decrease the success of treatment. Sputum conversion time is an indicator used to predict the treatment outcome. However, there is still no further study related to the association between DM comorbidity and the sputum conversion time in DR-TB patients, especially in Jember. This study aimed to determine the association between DM and sputum conversion time in DR-TB patients at Dr. Soebandi Regional General Hospital, Jember. Methods: This was an analytic observational study with a cross-sectional design. A total of 122 samples of DR-TB patients were taken using the purposive sampling method in 2018-2023 at Dr. Soebandi Regional General Hospital, Jember. The data were analyzed using the Chi-square and logistic regression statistical test. Results: Chi-square analysis showed that DM (p = 0.015; OR = 2.604; 95% CI 1.195-5.674) and age (p = 0.021; OR = 0.377; 95% CI 0.162-0.878) were associated with sputum conversion time. Logistic regression showed that DM was the most associated variable with the sputum conversion time (p = 0.016; OR = 2.604; 95% CI 1.195-5.674) compared to gender, age, TB resistance type, and anti-TB regimen. Conclusion: DM is associated with prolonged sputum conversion time in DR-TB patients at Dr. Soebandi Regional General Hospital, Jember. DM was also the most associated variable with the sputum conversion time compared to gender, age, TB resistance type, and anti-TB regimen.

ABSTRAK Sebagai tahap awal dalam siklus hidup tanaman kelapa sawit (Elaeis guineensis Jacq.), praktik pembibitan kelapa sawit menjadi aspek penting yang memerlukan evaluasi lebih dalam. Pemahaman mengenai dampak lingkungan pada fase pembibitan adalah kunci untuk merancang strategi pengelolaan yang berkelanjutan, mengurangi kerusakan ekosistem, dan mencapai keseimbangan antara produksi dan konservasi alam. Penelitian ini bertujuan untuk menganalisis dampak lingkungan pembibitan kelapa sawit di Unit Pembibitan Kelapa Sawit (UPKS) Politeknik Negeri Lampung menggunakan pendekatan penilaian daur hidup atau life cycle assessment (LCA). Studi ini difokuskan pada penilaian daur hidup gate-to-gate yang dilaksanakan dari Oktober hingga Desember 2023. Data dikumpulkan melalui kunjungan lapang dan wawancara dengan pengelola UPKS. Ruang lingkup LCA dimulai dari penerimaan kecambah hingga bibit siap salur. Permodelan dampak lingkungan menggunakan openLCA versi 2.0.3 dengan metode CML-IA baseline dan database pendukung Agribalyse v3.0.1. Hasil penelitian menunjukkan bahwa secara umum pembibitan kelapa sawit memberikan dampak lingkungan yang tidak signifikan. Kontribusi dampak lingkungan terbesar berasal dari dampak sekunder, khususnya marine aquatic ecotoxicity sebesar 190.52122 kg 1,4-DB eq. dan abiotic depletion (fossil fuels) sebesar 5.65733 MJ. Dampak primer potensi global warming (GWP100a) sebesar 0.27033 kg CO2 eq. yang dominan berasal dari penggunaan polybag dan potensi human toxicity sebesar 0.08227 kg 1,4-DB eq. Upaya pengurangan dampak lingkungan dapat dilakukan dengan penggunaan pot tray yang dapat digunakan berulang kali atau alternatif polybag dari bahan organik, kombinasi pupuk anorganik dan pupuk organik untuk mengurangi dosis pupuk anorganik, dan mengurangi laju evapotranpirasi dari tanaman dan media tanam dengan penggunaan mulsa organik dan media tanam yang mengandung bahan organic. Kata kunci: dampak lingkungan, kelapa sawit, LCA, pemanasan global, pembibitan ABSTRACT As the initial stage in the oil palm (Elaeis guineensis Jacq.) plant life cycle, oil palm seedling cultivation becomes a crucial aspect that requires in-depth evaluation. Understanding the environmental impact at the seedling phase is critical to designing more sustainable management strategies, reducing ecosystem damage, and achieving a balance between production and conservation. This study aims to present an environmental impact analysis of oil palm seedling cultivation at the Oil Palm Nursery Unit (UPKS) of Politeknik Negeri Lampung using the life cycle assessment (LCA) approach. This study focuses on a gate-to-gate life cycle assessment conducted from October to December 2023. Data were collected through field visits and interviews with UPKS managers. The LCA scope covers from seed acceptance to ready-to-distribute seedlings. Environmental impact modeling used openLCA version 2.0.3 with the CML-IA baseline method and supporting database Agribalyse v3.0.1. Results indicate that oil palm seedling nursery generally has an insignificant environmental impact. The most substantial contributions come from secondary impacts, mainly marine aquatic ecotoxicity at 190.52122 kg 1,4-DB eq. and abiotic depletion (fossil fuels) at 5.65733 MJ. Primary impacts are potential global warming (GWP100a) at 0.27033 kg CO2 eq., primarily from polybag use, and potential human toxicity at 0.08227 kg 1,4-DB eq. Efforts to mitigate environmental impacts can be achieved by using reusable pot trays or organic material-based polybags as alternatives, coupled with a combination of inorganic and organic fertilizers to reduce the dosage of inorganic fertilizers. Additionally, curtailing evapotranspiration rates from plants and growing media can be accomplished by employing organic mulch and growing media enriched with organic matter. Keywords: environmental impact, global warming, LCA, nursery, oil palm

Background: Mathematics is not just a science for its own sake; it is a science that proves to be useful for most other sciences. Mathematical connections play a crucial role in helping students develop a deeper understanding and enhance their thinking about mathematics. These connections represent relationships between different mathematical ideas, between mathematics and other subjects, and between mathematics and everyday life. Aim: This study aims to classify students' mathematical connection abilities when solving non-routine mathematical problems. Method: This research employs a qualitative approach using descriptive and exploratory methods. The study involved 23 students who exhibited mathematical connection skills in problem-solving. Data collection methods included tests, observations, and interviews. The data analysis process consisted of two main stages: data reduction and data presentation. To ensure data validation, the triangulation method was used by comparing the results from the subject tests (answer sheets), observations, and interviews. Results: The findings of this study revealed three distinct classifications of students’ mathematical connections when solving non-routine problems: 1) Patterns; 2) Variables; and 3) Arguments Conclusion: The implications of these findings suggest that teachers need to be aware of the different classifications of students' mathematical connections. This awareness will enable them to implement mathematics learning strategies that cater to the diverse mathematical connection classifications found in their classrooms. For instance, teachers can utilize different mathematics learning media tailored to students’ learning styles, such as mind, incubation, and visual learning. By doing so, students can better optimize their mathematical connections and achieve improved outcomes in their mathematics learning.

Currently, the political transaction through trade-off anak adat (customary child) status is wider applied in Papua local election. Benefiting from the direct elections, the elites competed for political power in the regions by mobilizing ethno-religious sentiments and utilizing money-politics. The voters, in return, behave as clients as they seek to gain material benefits from their preferred candidates in exchange for political loyalty. This condition caused the high-cost politics during the local election. Based on this problem, this paper attempts to analyze the hijacking of adat (customs) through trade-off anak adat status as a form of political transaction which impacted to high-cost politics. This study cultivates the theory of political transactions to analyze the political transaction in Papua local election especially in the gubernatorial election 2018 and election of DPRD (Regional Representative Council)’s member in 2019. This form of political transaction has caused the high-cost of local election in Papua, which is not only in the form of money, but also carried the social cultural high-cost impact. Of course, vote buying will make the candidates have to spend a lot of money. The trade-off of anak adat status from other than Papuan through buying and selling mechanism rather than using achievement and acknowledgement from the adat community would harm the Papuans. Moreover, this practice has brought detrimental destruction to the socio-cultural values of Papuan adat community. This study is qualitative research that use political approach to observe the local election processes in Papua. We used the criteria of credibility and confirmability to validate the data in our qualitative research.

Diazinon is an organophosphate pesticide commonly used by farmers. The use of diazinon can leave residues on agricultural products. Consumption of these products in the long term will cause health problems through oxidative stress mechanisms. Onion skin (Allium et al.) has a high content of flavonoids, which can neutralize oxidative stress. This study aims to determine the effectiveness of onion peel ethanol extract (OPEE) in reducing the plasma MDA level in diazinon-induced Wistar rats. Method This study is true experimental with a posttest-only control group design in vivo, using 28 samples divided based on a simple random sampling technique into four groups, i.e. the control group (K0), diazinon group (K1), OPEE groups P1 (300 mg/kgBW), P2 (600 mg/kgBW), P3 (900 mg/kgBW), P4 (1200 mg/kgBW), and P5 (2400 mg/kg BW). The examination of plasma MDA levels was carried out using the TBARS method. OPEE at doses of 300 mg/kgBW/day, 600 mg/kgBW/day, 900 mg/kgBW/day, 1200 mg/kgBW/day, and 2400 mg/kgBW/day significantly reduced the plasma MDA levels in diazinon-induced Wistar rats (p

Diazinon, an organophosphate pesticide, is used extensively in agricultural sector. Consumption of agricultural products containing diazinon residue may lead to harmful health consequences. Among these is nephrotoxicity, which includes lipid peroxidation, that can damage the kidney. Flavonoids in shallot peel can scavenge free radicals, inhibit necrosis, and activate bone marrow-derived cells for cell regeneration. This study aimed to determine the correlation between shallot peel infusion (SPI) dose and kidney damage amelioration to establish the maximum effective dose of SPI to ameliorate kidney histopathological damage in diazinon-induced rats. This study was performed at the Pharmacology Laboratory, Faculty of Medicine, University of Jember, Indonesia, in April 2021, on 35 rats that were divided into 7 groups—normal, diazinon, and five treatment groups. Diazinon 40 mg/kgBW was administered on day 1-7, while SPI 125, 250, 500, 1,000, 2,000 mg/kgBW were administered on day 8–14 according to the treatment group. Kidney histopathological slides with hematoxylin-eosin (H.E.) staining were assessed using Kocoglu scoring and Kidney damage scores of the treatment groups were analyzed using Pearson test. The maximum effective dose was determined using regression test. The damages found in diazinon-induced rats were tubular degeneration, necrosis, and inflammation with a higher damage score than normal rats (p

Diazinon merupakan pestisida organofosfat yang paling umum digunakan untuk mengendalikan hama. Konsumsi produk pertanian yang mengandung residu diazinon mengakibatkan akumulasi diazinon dan metabolitnya pada hati dan menimbulkan stress oksidatif. Kulit bawang merah (Allium cepa L.) mengandung flavonoid kuersetin yang dapat mengikat radikal bebas, menginduksi aktivitas enzim yang berperan penting dalam perbaikan sel, dan meningkatan jumlah dan kapasitas antioksidan endogen. Penelitian ini bertujuan mengetahui efek ekstrak ethanol kulit bawang merah (EKBM) dalam memperbaiki kerusakan hati tikus Wistar yang diinduksi diazinon. Tikus dibagi menjadi kelompok normal, diazinon, dan EKBM. Diazinon diberikan dengan dosis 40 mg/kgBB selama 7 hari dilanjutkan dengan EKBM dengan dosis 600, 900, dan 1200 mg/kgBB selama 7 hari. Pengamatan mikroskopik dilakukan terhadap 100 sel hati secara acak pada setiap preparat. Skor kerusakan sel hati ditentukan berdasarkan kriteria skor Manja Roenigk. Hasil penelitian menunjukkan bahwa diazinon meningkatkan skor kerusakan sel hati (p

Diazinon is an organophosphate pesticide often used by farmer. The residue of diazinon may contaminate agricultural products and if accidentally consumed in a long term, may potentially led to health problems such as kidney disorders. Shallot (Allium cepa) peel was recently proposed to help recover the kidney damage due to its high flavonoid antioxidant content. This study aims to determine the antioxidant effect of shallot peel extract in reducing oxidative stress caused by diazinon with malondialdehyde (MDA) level indicator on the kidney of Wistar rats (Rattus norvegicus). This research was a true experimental with an in-vivo posttest-only control group design. The 28 rats were divided using a simple random sampling technique. The groups were the control group (K0), the diazinon group (K1), SPEE (Shallot Peel Ethanol Extract) groups which are P1 (300 mg/kg BW), P2 (600 mg/kg BW), P3 (900 mg/kg BW), P4 (1200 mg/kg BW) and P5 (2400 mg/kg BW). At the end of the study, the MDA kidney levels was analysed using the TBARS method. Effective doses were found in 600 mg/kg BW/day, 900 mg/kg BW/day, 1200 mg/kg BW/day, and 2400 mg/kg BW/day. The optimal dose of shallot peel extract in this study was 600 mg/kg BW/day. This study showed the effectiveness of shallot peel extract in reducing kidney MDA levels in diazinon-induced Wistar rats. Keywords: shallot peel, diazinon, kidney MDA level

Aspartame is an artificial sweetener in foods and beverages. Continuous use of aspartame will result in oxidative stress in the liver. So we need antioxidants from outside the body such as periwinkle leaves.This study aims to determine the effect of periwinkle leaves (Catharanthus roseus L) on aspartame-induced liver histology. This study used 24 male mice divided into 4 groups, namely negative control (K-) without treatment; Positive control (K +) treatment of aspartame; D1: treatment of aspartame and periwinkle extract doses of 0.42 mg/kg. D2: aspartame and periwinkle extract doses of 0.84 mg/kgbb. Administration of aspartame dose of 0.2 mg /kgbb by intraperitoneal for 14 days followed by gavage administration of periwinkle leaf extract for 12 days. The liver was taken on the 27th day by surgery, then made slide histology with Hematoxylin-Eosin staining. The results show that the administration of Catharanthus roseus leaf extract at a dose of 0.84 mg/kgbw in aspartame-induced mice was effective in reducing hepatocyte damage, namely hydrophilic degeneration and necrosis.

Indonesia is the largest archipelagic country in the world, with 17,000 islands of varying sizes and elevations, from lowlands to very high mountains, stretching more than 5000 km eastward from Sabang in Aceh to Merauke in Papua. Although occupying only 1.3% of the world’s land area, Indonesia possesses the third-largest rainforest and the second-highest level of biodiversity, with very high species diversity and endemism. However, during the last two decades, Indonesia has been known as a country with a high level of deforestation, a producer of smoke from burning forests and land, and a producer of carbon emissions. The aim of this paper is to review the environmental history and the long process of Indonesian forest management towards achieving environmental sustainability and community welfare. To do this, we analyze the milestones of Indonesian forest management history, present and future challenges, and provide strategic recommendations toward a viable Sustainable Forest Management (SFM) system. Our review showed that the history of forestry management in Indonesia has evolved through a long process, especially related to contestation over the control of natural resources and supporting policies and regulations. During the process, many efforts have been applied to reduce the deforestation rate, such as a moratorium on permitting primary natural forest and peat land, land rehabilitation and soil conservation, environmental protection, and other significant regulations. Therefore, these efforts should be maintained and improved continuously in the future due to their significant positive impacts on a variety of forest areas toward the achievement of viable SFM. Finally, we conclude that the Indonesian government has struggled to formulate sustainable forest management policies that balance economic, ecological, and social needs, among others, through developing and implementing social forestry instruments, developing and implementing human resource capacity, increasing community literacy, strengthening forest governance by eliminating ambiguity and overlapping regulations, simplification of bureaucracy, revitalization of traditional wisdom, and fair law enforcement.

Background: Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) is an important protein responsible for the pathogenesis of severe malaria, including cerebral malaria. The protein is highly diverse. The CIDR1α-PfEMP1 binds endothelial protein receptor (EPCR) and may associated with the brain swelling in childhood malaria. Objective: To analyze the CIDR1α-PfEMP1 from Indonesian isolate and determine its association with cerebral malaria outcome. Methods: Fifteen blood samples of clinically mild to severe malaria-patient were collected for DNA extraction. Malaria diagnosis was conducted microscopically by Giemsa-stained thin blood smear. The CIDR1α domain was amplified by PCR using specific primer and PCR product was sequenced. The nucleotide sequences were analyzed by NCBI blast, DNASIS MAX 3 and translated into amino acid sequences using Expasy Translation Tool. Results: One out of fifteen samples was severe malaria case and infected with P. falciparum, the rest were clinically mild to moderate malaria and infected with pure P. falciparum or mixed infection of P. falciparum and P. vivax. Amplification for CIDR1α domain resulted a single band of + 550 bp from a severe sample only. Sequencing of PCR product on both strands read 524 nucleotides and BLAST analysis confirmed as CIDR1α sequence. Multiple alignment showed 74-78% nucleotide sequence similarity with reference sequences, but amino acid sequences presented 23.5% homologous. Conclusion: An identified CIDR1α domain only from severe case implicating the potential association with the severe outcome including cerebral malaria, but the highly diverse of the domain needs further studies on the interaction with the pathological-causing receptor in the host.

ABSTRACT Diazinon has a hepatotoxic effect since it is metabolized in the liver involving Kupffer cells (KCs) activation, increasing reactive oxygen species (ROS). The flavonoids contained in shallot (Allium cepa L.) skin act as antioxidants neutralizing oxidative stress. This study aims to determine the effect of shallot skin ethanol extract (SSEE) on liver MDA level and activated KCs histopathology. The total flavonoids level of SSEE was measured using the aluminum chloride colorimetric method and resulted in 228.1 mg QE/g. Rats were divided into normal, diazinon, and SSEE groups. Diazinon was administered at a dose of 40 mg/kg b.w. for 7 days, followed by SSEE at the dose of 600, 900, and 1,200 mg/kg b.w. for 7 days. Liver malondialdehyde (MDA) level was measured using the MDA-TBA method. The results revealed that diazinon increased liver MDA level (p 0.05). Activated KCs in the SSEE group at a dose of 1,200 mg/kg b.w. was impressively equal to the normal group. In conclusion, SSEE at dose of 1,200 mg/kg b.w. neutralize liver oxidative stress due to diazinon indicated by the decrease of liver MDA level and activated KCs equal to normal. Keywords: antioxidant, diazinon, Kupffer cell, liver, MDA, shallot Correspondence: rosita.fk@unej.ac.id

Most of the Indonesian agarwood in the international market is harvested from the wild, which raises concerns regarding its sustainability. The Government of Indonesia encourages agarwood cultivation produced from the cultivated Agarwood-Producing Tree (APT) to overcome this concern and replace natural agarwood. APT cultivation in Indonesia is not a new development, but it has faced various obstacles, ranging from production quantity and quality to funding and marketing. Therefore, an appropriate policy is needed to support the success of APT cultivation. This study aims to develop a dynamic system model in order to identify the gaps and determine appropriate policy strategies to improve APT cultivation in Indonesia. The model was established by compiling three conceptual stages: planting to harvest, cost–benefit analysis, and feasibility analysis. Agarwood from Aquilaria malaccensis Lam. cultivated by the community and private sector, which produces kemedangan (an agarwood grade in the Indonesian market) and oil, was chosen for the model. The model developed shows that APT cultivation development in the private sector and the community is unfeasible with the business as usual. There are three options to simulate the feasibility of agarwood produces from APT cultivation. The best scenarios are chosen based on the feasibility indicator, when benefit is higher than cost. The development of APT by the private sector that produces kemedangan and oil products is feasible, with the invention of more effective inoculant and processing technology (scenario 1), as well as applying high thinning, which can increase the yield. Oil production requires more investment, so the revenue obtained is lower than the production cost, resulting in the unfeasibility of the production. The development of APT by the community will be feasible with scenario 2, if there is government funding for the establishment of APT cultivation, inoculants application, and harvesting. Based on the model scenario, APT cultivation will be ecologically sustainable, economically feasible, and socially acceptable if carried out by the private sector or the community by applying inoculation techniques and selecting inoculants to increase production effectiveness, and will be supported by lower production costs and market stability. The Indonesian government needs to take several policies to encourage APT development, including financial assistance for APT development, setting inoculant standards at affordable prices, simplifying trade administration, stabilizing agarwood product prices at the local level, and law enforcement.

The domestic silkworm or mulberry silk moth, B. mori L., provides more than 99% of the world’s silk. Silk, as a sericulture product, was first introduced in Indonesia through a trade mechanism and began to develop in 1953. Several factors (economic, ecological, market, and cultural) support sericulture and make it become one of the non-timber forest product priorities. However, the competitive advantages alone have not encouraged the development of prospective sericulture industry in Indonesia yet. This paper is a review of tropical sericulture development in Indonesia. The literature on the development of sericulture in Indonesia between 1989 and 2022 is used to describe conditions related to mulberry cultivation (moriculture), and silkworm rearing (sericulture), as well as the state of socio-economic development, culture, and institutions. Moriculture and sericulture techniques, socio-economic aspects, institutional arrangements, and community motivations are intertwined, creating a challenging atmosphere for sericulture development. There are potential resources, such as exploring quality mulberry production and quality silkworm production through research and development, valuable cultural aspects, and potential stakeholders to build network engagement. Commitment, cooperation, and action from all stakeholders are needed to enhance the development of sericulture in Indonesia. In this context, the central government can play an important role in facilitating multi-stakeholder partnerships in the development of integrated sericulture in Indonesia.

The malaria vaccine is an important strategy for the global malaria elimination program, but the complexity of the Plasmodium antigen is a major hurdle in malaria vaccine development. The cysteine-rich interdomain region 1α (CIDR1α) of Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is crucial in malaria pathogenesis, making it a vaccine candidate. This study investigated the leukocyte and IgM response generated after administering a CIDR1α-PfEMP1 recombinant protein injection in Wistar rats. The rats were divided into a control group, who received a physiological saline solution (PSS), and a treatment group, who were subcutaneously injected with 150 µg of purified CIDR1α-PfEMP1 protein three times at the 3-week interval. Blood samples were collected every week after each injection. The number of leukocytes were counted using a Neubauer chamber, and the IgM concentration was determined using an enzyme-linked immunosorbent assay (ELISA). Data were analyzed using an independent, paired-T test, a Mann–Whitney test, and a Wilcoxon test, based on the distribution of the data. The total number of leukocytes notably increased on day 29 (p < 0.05). The percentage of neutrophils decreased, especially on day 8 (p < 0.05), whereas the percentages of monocytes and lymphocytes increased, primarily on day 14 (p < 0.05). The IgM concentration increased on day 14 (p < 0.05). In conclusion, the CIDR1α-PfEMP1 recombinant protein may induce leukocyte and IgM responses, making it a potential malaria vaccine candidate.

Abstract Gentamicin usage can cause the damage of liver structure and function. The basic mechanism inducing liver damage from gentamicin is lipid peroxidation in cell membrane and the suppresion of antioxidant defence system in liver. Antioxidant in cassava leaf such as vitamin C, carotene, flavonoid, dan mineral can protect liver from drug toxicity effect. This research aimed to determine hepatoprotective effect of cassava leaf through microscopic observation of liver histopathology slide of mice induced by gentamicin. The research design was post test only control group design. Mice were divided into five groups, normal group, positive control (gentamicin 80 mg/kg b.w.); P1, P2, and P3 (gentamicin 80 mg/kg b.w. and cassava leaf extract 150 mg/kg b.w., 300 mg/kg b.w., 450 mg/kg b.w. respectively, for 14 days). The average score of liver cell damage was determined by microscopic observation of 200 liver cells undergoing parenchymal degeneration, hidrophic degeneration, and necrosis. One Way Anova analysis showed significant difference among the groups (p

Drugs consumption like gentamicin caused damage and decreased of kidney function. Cassava leaf contain lots of micronutrients, such as vitamin C, vitamin A and carotenoids, which also as an antioxidant. The results of the study conducted by Almashuri proved that cassava leaf have high levels of carotenoid compounds. The purpose of this study was to determine the effect of cassava leaf extract on the repairment of renal damage in mice in vivo. This research is true experimental using mice (mus musculus) and were grouped into 5 groups. Two control groups (positive and negative) and 3 treatment groups (cassava leaves 150, 300, and 450 mg / day). Gentamicin is given every day for 14 days and then observed on serum levels of creatinine along with histopathological scores of kidneys’ damage. Statistical analysis showed a significant reduction in serum levels of creatinine (P

Participatory mapping has recently become an instrument used by NGOs to advocate for adat (customary) land in Indonesia. Maps produced from participatory mapping are expected to support legal recognition through land formalization or titling. In order to stop land grabbing through the Merauke Integrated Food and Energy Estate (MIFEE) project, this strategy has also been applied in Merauke district, Papua. However, the pitfalls of communal participatory mapping have brought negative impacts to adat communities. This paper analyzes the land grabbing and mapping processes in three villages in the MIFEE area to show the unexpected consequences of participatory mapping. These mapping processes have caused fragmentation and conflict among adat communities. Furthermore, the legal recognition of communal adat land ownership is facilitating the buy-out of adat land by companies and/or the state.

Yogyakarta is one of the destinations in Indonesia affected by the Covid-19. Currently, tourist objects in Yogyakarta are gradually starting to open to the public, and one of them is beach tourism, although it can lead to a new cluster risk of the spread of Covid-19. According to the Texas Medical Association, traveling to a beach is included in a moderate risk with a score of 5-6, and health protocols are still required. Applying the crisis management and crisis communication model of Coombs, J, Holladay, this study analyzes how tourism actors manage crisis communication at the beaches of Yogyakarta in the new normal era. A qualitative case study approach was used to illustrate that crisis communication management is an effort to manage a crisis to provide solutions to the beach tourism due to the Covid-19. Crisis management consists of stages, for example, pre-crisis, crisis, and post-crisis. Through these stages, tourism actors and tourists can determine steps they must prepare for during the Covid-19 crisis. In order to resolve the crisis during the pandemic, it is necessary to adopt new habits by tourism actors and tourists. New habits need to be communicated directly or through existing media because communication is essential in crisis resolution.

Tujuan penelitian ini adalah untuk menganalisis kemandirian belajar siswa. Jenis penelitian ini yaitu penelitian deskriptif kualitatif. Subjek penelitian ini diambil dengan teknik purposive sampling dan didapatkan 10 siswa yang menjadi subjek. Teknik pengumpulan data yaitu angket. Teknik analisis data dalam penelitian ini meliputi mereduksi data dan menentukan persentase data, penyajian data, dan kesimpulan. Hasil penelitian diperoleh 43,96% siswa memenuhi indikator percaya diri, 52% siswa memenuhi indikator bertanggung jawab, 58,33% siswa memenuhi indikator dapat memecahkan masalahnya sendiri, 49% siswa memenuhi indikator dapat merencanakan kegiatan belajar sendiri, 46,33% siswa memenuhi indikator bersikap aktif, 41,33% siswa memenuhi indikator tidak mudah terpengaruh oleh orang lain, dan 43% siswa memenuhi indikator kesadaran untuk belajar sendiri.

Innovations in food biotechnology reflect the increasing demand for healthy food and the change in conventional dietary patterns to plant-based diets, encouraging the development of functional products and opening new perspectives for industry interests. In addition, the development of vegetable-based products is supported by several studies that state that plant-based diets help reduce the risk of diseases, reduce stress, and even help maintain healthy body weight, making this diet a promising development for the future. The industry mainly uses fermentation techniques to obtain plant-based foodstuffs. However, fermentation is just one method that can develop probiotic products. Other methods include high moisture extrusion cooking and applying shear cells, for instance, for developing vegetable meats. This article summarizes trends and shifts in eating preferences, the response of the respective industry, and the future potential of plant-based products.

Microbial contamination may cause food poisoning and a decrease in food quality. Preservatives such as Citrus senensis sweet orange peel waste can be used to extend the shelf life of food. The purpose of this study was to measure the potential antioxidant and antibacterial activity of orange peel extract and its application to improve the quality of meatballs, wet noodles, and tofu. Meatballs, wet noodles, and tofu were soaked with the extract for 90 minutes prior to storage at room temperature (28±2°C) for 4 days. The results showed that the inhibition zone of the extract using disc diffusion assay was 17.67±0.58 mm against Escherichia coli, 16±1 mm against Staphylococcus aureus, and 12.67±1.15 mm against Salmonella Typhi. Sweet orange peel extract had an antioxidant activity of 66.41% with total phenol of 2,656.48±55.46 mg GAE/100g. The extract was able to reduce total bacteria between 0.5 and 1.0 log CFU/g in meatballs, wet noodles and tofu. The application of the extract on meatballs, wet noodles and tofu preserved aroma and color attribute until 4th day of storage at room temperature (28±2°C). Decrease of texture attributes were observed starting from 3rd day of storage at room temperature (28±2°C).

ABSTRACT This study aims to empirically test the market reaction that occurs upon the announcement of Economic Policy Package X about the negative investment list (DNI). This study using event study method. The market reaction is calculated using cumulative abnormal return (CAR). The population used are all companies listed on the Indonesian Stock Exchange (BEI), 525 companies. The number of samples used are 477 companies. The analysis technique used is the one sample t-test and the independent sample t-test. The analysis showed that there is a positive market reaction to the announcement of the Economic Policy Package X. Furthermore, there are differences in the reaction that occurs between the business sectors that benefited by the policy than other business sectors. The highest reaction is shown by the business sectors that benefited by the policy. Business sectors which are benefited by the policy announcement obtained higher market reaction than their counterpart. Keywords: Market reaction, economic policy package X, negative list investment, abnormal return