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Adventitious shoots and roots were regenerated from leaf segments of 3 Solanum species: S. candidum Lindl., S. quitoense Lam. and S. sessiliflorum Dunal. Leaf explants differentiated shoots on modified MS medium supplemented with 23-163 µm kinetin and 0-5.7 µm indoleacetic acid (IAA). Excised shoots were induced to form roots by transfer to media with benzyladenine (BA) and naphthaleneacetic acid (NAA) at 0.09 and 0.11/2 µM respectively for S. qultoense and 0.01 µm NAA for S. candidum and S. sessiliflorum. Adventitious roots were produced directly from leaf explants with 0-140 µm kinetin and 0-5.7µm IAA in combination. Rooted plants were successfully established in the greenhouse.