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194 results on '"Rosati, Emanuela"'

1. A pro-inflammatory environment in bone marrow of Treg transplanted patients matches with graft-versus-leukemia effect

Author

Guardalupi, Francesco, Sorrentino, Carlo, Corradi, Giulia, Giancola, Raffaella, Baldoni, Stefano, Ulbar, Francesca, Fabi, Bianca, Andres Ejarque, Rosa, Timms, Jessica, Restuccia, Francesco, Santarone, Stella, Accorsi, Patrizia, Sportoletti, Paolo, De Falco, Filomena, Rosati, Emanuela, Carotti, Alessandra, Falzetti, Franca, Velardi, Andrea, Martelli, Massimo Fabrizio, Kordasti, Shahram, Pierini, Antonio, Ruggeri, Loredana, and Di Ianni, Mauro

Published
2023
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3. NOTCH1 inhibition prevents GvHD and maintains GvL effect in murine models

Author

Baldoni, Stefano, Ruggeri, Loredana, Del Papa, Beatrice, Sorcini, Daniele, Guardalupi, Francesco, Ulbar, Francesca, Marra, Andrea, Dorillo, Erica, Stella, Arianna, Giancola, Raffaella, Fabi, Bianca, Sola, Rosaria, Ciardelli, Sara, De Falco, Filomena, Rompietti, Chiara, Adamo, Francesco Maria, Rosati, Emanuela, Pierini, Antonio, Sorrentino, Carlo, Sportoletti, Paolo, and Di Ianni, Mauro

Published
2021
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4. NOTCH1-mutated chronic lymphocytic leukemia displays high endoplasmic reticulum stress response with druggable potential

Author

Silva Barcelos, Estevão Carlos, primary, Rompietti, Chiara, additional, Adamo, Francesco Maria, additional, Dorillo, Erica, additional, De Falco, Filomena, additional, Del Papa, Beatrice, additional, Baldoni, Stefano, additional, Nogarotto, Manuel, additional, Esposito, Angela, additional, Capoccia, Silvia, additional, Geraci, Clelia, additional, Sorcini, Daniele, additional, Stella, Arianna, additional, Arcaleni, Roberta, additional, Tini, Valentina, additional, Imbroisi Valle Errera, Flávia, additional, Rosati, Emanuela, additional, and Sportoletti, Paolo, additional

Published
2023
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5. Therapeutic Targeting Potential of Novel Silver Nanoparticles Coated with Anti-CD20 Antibody against Chronic Lymphocytic Leukemia

Author

Adamo, Francesco Maria, primary, Silva Barcelos, Estevao Carlos, additional, De Falco, Filomena, additional, Dorillo, Erica, additional, Rompietti, Chiara, additional, Sorcini, Daniele, additional, Stella, Arianna, additional, Del Papa, Beatrice, additional, Baldoni, Stefano, additional, Esposito, Angela, additional, Geraci, Clelia, additional, Arcaleni, Roberta, additional, Pennetta, Chiara, additional, Ragonese, Francesco, additional, Moretti, Lorenzo, additional, Mameli, Mariagrazia, additional, Di Ianni, Mauro, additional, Rosati, Emanuela, additional, Fioretti, Bernard, additional, and Sportoletti, Paolo, additional

Published
2023
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6. Therapeutic targeting of chronic lymphocytic leukemia by silver nanoparticles

Author

Adamo, Francesco Maria, primary, Barcelos, Estevao Carlos Silva, additional, Falco, Filomena, additional, Dorillo, Erica, additional, Rompietti, Chiara, additional, Sorcini, Daniele, additional, Stella, Arianna, additional, Papa, Beatrice Del, additional, Baldoni, Stefano, additional, Esposito, Angela, additional, Geraci, Clelia, additional, Arcaleni, Roberta, additional, Pennetta, Chiara, additional, Ragonese, Francesco, additional, Moretti, Lorenzo, additional, Mameli, Mariagrazia, additional, Ianni, Mauro, additional, Rosati, Emanuela, additional, Fioretti, Bernard, additional, and Sportoletti, Paolo, additional

Published
2023
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7. NOTCH1-mutated chronic lymphocytic leukemia displays high endoplasmic reticulum stress response with druggable potential.

Author

Barcelos, Estevão Carlos Silva, Rompietti, Chiara, Adamo, Francesco Maria, Dorillo, Erica, De Falco, Filomena, Del Papa, Beatrice, Baldoni, Stefano, Nogarotto, Manuel, Esposito, Angela, Capoccia, Silvia, Geraci, Clelia, Sorcini, Daniele, Stella, Arianna, Arcaleni, Roberta, Tini, Valentina, Valle Errera, Flávia Imbroisi, Rosati, Emanuela, and Sportoletti, Paolo

Subjects
CHRONIC lymphocytic leukemia, CHRONIC leukemia, ENDOPLASMIC reticulum, GENE ontology, UNFOLDED protein response, POLYMERASE chain reaction, B cells
Abstract

Introduction: Constitutive activation of NOTCH1-wild-type (NT1-WT) signaling is associated with poor outcomes in chronic lymphocytic leukemia (CLL), and NOTCH1 mutation (c.7541_7542delCT), which potentiates NOTCH1 signaling, worsens the prognosis. However, the specific mechanisms of NOTCH1 deregulation are still poorly understood. Accumulative evidence mentioned endoplasmic reticulum (ER) stress/unfolded protein response (UPR) as a key targetable pathway in CLL. In this study, we investigated the impact of NOTCH1 deregulation on CLL cell response to ER stress induction, with the aim of identifying new therapeutic opportunities for CLL. Methods: We performed a bioinformatics analysis of NOTCH1-mutated (NT1-M) and NT1-WT CLL to identify differentially expressed genes (DEGs) using the rank product test. Quantitative real-time polymerase chain reaction (qPCR), Western blotting, cytosolic Ca2+, and annexin V/propidium iodide (PI) assay were used to detect curcumin ER stress induction effects. A median-effect equation was used for drug combination tests. The experimental mouse model E -TCL1 was used to evaluate the impact of ER stress exacerbation by curcumin treatment on the progression of leukemic cells and NOTCH1 signaling. Results and discussion: Bioinformatics analysis revealed gene enrichment of the components of the ER stress/UPR pathway in NT1-M compared to those in NT1- WT CLL. Ectopic expression of NOTCH1 mutation upregulated the levels of ER stress response markers in the PGA1 CLL cell line. Primary NT1-M CLL was more sensitive to curcumin as documented by a significant perturbation in Ca2+ homeostasis and higher expression of ER stress/UPR markers compared to NT1-WT cells. It was also accompanied by a significantly higher apoptotic response mediated by C/EBP homologous protein (CHOP) expression, caspase 4 cleavage, and downregulation of NOTCH1 signaling in NT1-M CLL cells. Curcumin potentiated the apoptotic effect of venetoclax in NT1-M CLL cells. In E -TCL1 leukemic mice, the administration of curcumin activated ER stress in splenic B cells ex vivo and significantly reduced the percentage of CD19+/CD5+ cells infiltrating the spleen, liver, and bone marrow (BM). These cellular effects were associated with reduced NOTCH1 activity in leukemic cells and resulted in prolonged survival of curcumin-treated mice. Overall, our results indicate that ER stress induction in NT1-M CLL might represent a new therapeutic opportunity for these high-risk CLL patients and improve the therapeutic effect of drugs currently used in CLL. [ABSTRACT FROM AUTHOR]

Published
2023
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8. Figure S10 from Decreased NOTCH1 Activation Correlates with Response to Ibrutinib in Chronic Lymphocytic Leukemia

Author

Del Papa, Beatrice, primary, Baldoni, Stefano, primary, Dorillo, Erica, primary, De Falco, Filomena, primary, Rompietti, Chiara, primary, Cecchini, Debora, primary, Cantelmi, Maria Grazia, primary, Sorcini, Daniele, primary, Nogarotto, Manuel, primary, Adamo, Francesco Maria, primary, Mezzasoma, Federica, primary, Silva Barcelos, Estevão Carlos, primary, Albi, Elisa, primary, Iacucci Ostini, Roberta, primary, Di Tommaso, Ambra, primary, Marra, Andrea, primary, Montanaro, Guido, primary, Martelli, Maria Paola, primary, Falzetti, Franca, primary, Di Ianni, Mauro, primary, Rosati, Emanuela, primary, and Sportoletti, Paolo, primary

Published
2023
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10. Immune correlates of protection by vaccine against SARS‐CoV ‐2 in patients with chronic lymphocytic leukaemia

Author

Sorcini, Daniele, primary, De Falco, Filomena, additional, Gargaro, Marco, additional, Bozza, Silvia, additional, Guarente, Valerio, additional, Cardinali, Valeria, additional, Stella, Arianna, additional, Adamo, Francesco Maria, additional, Silva Barcelos, Estevao Carlos, additional, Rompietti, Chiara, additional, Dorillo, Erica, additional, Geraci, Clelia, additional, Esposito, Angela, additional, Arcaleni, Roberta, additional, Capoccia, Silvia, additional, Mameli, Maria Grazia, additional, Graziani, Alessandro, additional, Moretti, Lorenzo, additional, Cipiciani, Alessandra, additional, Riccardi, Carlo, additional, Mencacci, Antonella, additional, Fallarino, Francesca, additional, Rosati, Emanuela, additional, and Sportoletti, Paolo, additional

Published
2022
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17. NK Cells in Chronic Lymphocytic Leukemia and Their Therapeutic Implications

Author

Sportoletti, Paolo, primary, De Falco, Filomena, additional, Del Papa, Beatrice, additional, Baldoni, Stefano, additional, Guarente, Valerio, additional, Marra, Andrea, additional, Dorillo, Erica, additional, Rompietti, Chiara, additional, Adamo, Francesco Maria, additional, Ruggeri, Loredana, additional, Di Ianni, Mauro, additional, and Rosati, Emanuela, additional

Published
2021
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18. NOTCH1 Activation Negatively Impacts on Chronic Lymphocytic Leukemia Outcome and Is Not Correlated to the NOTCH1 and IGHV Mutational Status

Author

Baldoni, Stefano, primary, Del Papa, Beatrice, additional, De Falco, Filomena, additional, Dorillo, Erica, additional, Sorrentino, Carlo, additional, Rompietti, Chiara, additional, Adamo, Francesco Maria, additional, Nogarotto, Manuel, additional, Cecchini, Debora, additional, Mondani, Elena, additional, Silva Barcelos, Estevao Carlos, additional, Moretti, Lorenzo, additional, Mameli, Maria Grazia, additional, Fabi, Bianca, additional, Sorcini, Daniele, additional, Stella, Arianna, additional, Giancola, Raffaella, additional, Guardalupi, Francesco, additional, Ulbar, Francesca, additional, Plebani, Sara, additional, Guarente, Valerio, additional, Rosati, Emanuela, additional, Di Nicola, Marta, additional, Marchioni, Michele, additional, Di Ianni, Mauro, additional, and Sportoletti, Paolo, additional

Published
2021
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19. Bepridil exhibits anti‐leukemic activity associated with NOTCH1 pathway inhibition in chronic lymphocytic leukemia

Author

Baldoni, Stefano, Del Papa, Beatrice, Dorillo, Erica, Aureli, Patrizia, De Falco, Filomena, Rompietti, Chiara, Sorcini, Daniele, Varasano, Emanuela, Cecchini, Debora, Zei, Tiziana, Di Tommaso, Ambra, Rosati, Emanuela, Alexe, Gabriela, Roti, Giovanni, Stegmaier, Kimberly, Di Ianni, Mauro, Falzetti, Franca, and Sportoletti, Paolo

Subjects
Bepridil, Antineoplastic Agents, Apoptosis, Mice, NOTCH1, hemic and lymphatic diseases, Biomarkers, Tumor, Tumor Microenvironment, Animals, Humans, Receptor, Notch1, Cancer Therapy and Prevention, Chemotaxis, Mesenchymal Stem Cells, targeted therapy, Calcium Channel Blockers, Prognosis, Leukemia, Lymphocytic, Chronic, B-Cell, Xenograft Model Antitumor Assays, embryonic structures, Mutation, cardiovascular system, chronic lymphocytic leukemia, sense organs, biological phenomena, cell phenomena, and immunity, Drug Screening Assays, Antitumor
Abstract

Dysregulated NOTCH1 signaling, by either gene mutations or microenvironment interactions, has been increasingly linked to chronic lymphocytic leukemia (CLL). Thus, inhibiting NOTCH1 activity represents a potential therapeutic opportunity for this disease. Using gene expression‐based screening, we identified the calcium channel modulator bepridil as a new NOTCH1 pathway inhibitor. In primary CLL cells, bepridil induced selective apoptosis even in the presence of the protective stroma. Cytotoxic effects of bepridil were independent of NOTCH1 mutation and other prognostic markers. The antitumor efficacy of bepridil was associated with inhibition of NOTCH1 activity through a decrement in trans‐membrane and activated NOTCH1 protein levels with unchanged NOTCH2 protein levels. In a CLL xenotransplant model, bepridil significantly reduced the percentage of leukemic cells infiltrating the spleen via enhanced apoptosis and decreased NOTCH1 activation. In conclusion, we report in vitro and in vivo anti‐leukemic activity of bepridil associated with inhibition of the NOTCH1 pathway in CLL. These data provide a rationale for the clinical development of bepridil as anti‐NOTCH1 targeted therapy for CLL patients., What's new? In search of new inroads against chronic lymphocytic leukemia, these authors turned to the NOTCH1 signalling pathway. Could inhibiting NOTCH1 help treat CLL? To find out, they first identified the calcium channel blocker bepridil as a NOTCH1 inhibitor. Next, they showed that bepredil induced apoptosis in isolated CLL cells, regardless of whether they bore NOTCH1 mutations. In mice bearing CLL cells, bepredil inhibited the proliferation of the tumor cells with no overt signs of toxicity. Thus, it seems advisable to pursue bepredil as a possible candidate for treating CLL.

Published
2018

21. NOTCH1 PEST domain mutation is an adverse prognostic factor in B-CLL

Author

Sportoletti, Paolo, Baldoni, Stefano, Cavalli, Laura, Del Papa, Beatrice, Bonifacio, Elisabetta, Ciurnelli, Raffaella, Bell, Alain Sylvin, Di Tommaso, Ambra, Rosati, Emanuela, Crescenzi, Barbara, Mecucci, Cristina, Screpanti, Isabella, Marconi, Pierfrancesco, Martelli, Massimo F., Di Ianni, Mauro, and Falzetti, Franca

Published
2010
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22. Decreased NOTCH1 Activation Correlates with Response to Ibrutinib in Chronic Lymphocytic Leukemia

Author

Del Papa, Beatrice, primary, Baldoni, Stefano, additional, Dorillo, Erica, additional, De Falco, Filomena, additional, Rompietti, Chiara, additional, Cecchini, Debora, additional, Cantelmi, Maria Grazia, additional, Sorcini, Daniele, additional, Nogarotto, Manuel, additional, Adamo, Francesco Maria, additional, Mezzasoma, Federica, additional, Silva Barcelos, Estevão Carlos, additional, Albi, Elisa, additional, Iacucci Ostini, Roberta, additional, Di Tommaso, Ambra, additional, Marra, Andrea, additional, Montanaro, Guido, additional, Martelli, Maria Paola, additional, Falzetti, Franca, additional, Di Ianni, Mauro, additional, Rosati, Emanuela, additional, and Sportoletti, Paolo, additional

Published
2019
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25. Graft engineering for allogeneic haploidentical stem cell transplantation

Author

Tabilio, Antonio, Falzetti, Franca, Zei, Tiziana, De Ioanni, Maria, Bonifacio, Elisabetta, Battelli, Feliciana, Iacucci Ostini, Roberta, Ballanti, Stelvio, Cimminiello, Michele, Capponi, Monia, Silvani, Carla, Minelli, Olivia, Fettucciari, Katia, Marconi, Pierfrancesco, Rosati, Emanuela, Santucci, Antonella, Di Ianni, Mauro, Aversa, Franco, and Martelli, Massimo F.

Published
2004
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29. NOTCH and Graft-Versus-Host Disease

Author

Di Ianni, Mauro, primary, Del Papa, Beatrice, additional, Baldoni, Stefano, additional, Di Tommaso, Ambra, additional, Fabi, Bianca, additional, Rosati, Emanuela, additional, Natale, Annalisa, additional, Santarone, Stella, additional, Olioso, Paola, additional, Papalinetti, Gabriele, additional, Giancola, Raffaella, additional, Accorsi, Patrizia, additional, Di Bartolomeo, Paolo, additional, Sportoletti, Paolo, additional, and Falzetti, Franca, additional

Published
2018
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30. NOTCH1 Aberrations in Chronic Lymphocytic Leukemia

Author

Rosati, Emanuela, primary, Baldoni, Stefano, additional, De Falco, Filomena, additional, Del Papa, Beatrice, additional, Dorillo, Erica, additional, Rompietti, Chiara, additional, Albi, Elisa, additional, Falzetti, Franca, additional, Di Ianni, Mauro, additional, and Sportoletti, Paolo, additional

Published
2018
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31. NOTCH1 Is Aberrantly Activated in Chronic Lymphocytic Leukemia Hematopoietic Stem Cells

Author

Di Ianni, Mauro, primary, Baldoni, Stefano, additional, Del Papa, Beatrice, additional, Aureli, Patrizia, additional, Dorillo, Erica, additional, De Falco, Filomena, additional, Albi, Elisa, additional, Varasano, Emanuela, additional, Di Tommaso, Ambra, additional, Giancola, Raffaella, additional, Accorsi, Patrizia, additional, Rotta, Gianluca, additional, Rompietti, Chiara, additional, Silva Barcelos, Estevão Carlos, additional, Campese, Antonio Francesco, additional, Di Bartolomeo, Paolo, additional, Screpanti, Isabella, additional, Rosati, Emanuela, additional, Falzetti, Franca, additional, and Sportoletti, Paolo, additional

Published
2018
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32. Subclones in B-lymphoma cell lines: isogenic models for the study of gene regulation

Author

Quentmeier, Hilmar, primary, Pommerenke, Claudia, additional, Ammerpohl, Ole, additional, Geffers, Robert, additional, Hauer, Vivien, additional, MacLeod, Roderick AF, additional, Nagel, Stefan, additional, Romani, Julia, additional, Rosati, Emanuela, additional, Rosén, Anders, additional, Uphoff, Cord C, additional, Zaborski, Margarete, additional, and Drexler, Hans G, additional

Published
2016
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33. Notch1 modulates mesenchymal stem cells mediated regulatory T-cell induction

Author

DEL PAPA, Beatrice, Sportoletti, Paolo, Cecchini, Debora, Rosati, Emanuela, Balucani, Chiara, Baldoni, Stefano, Fettucciari, Katia, Marconi, Pierfrancesco, Martelli, Massimo Fabrizio, Falzetti, Franca, and DI IANNI, Mauro

Subjects
Homeodomain Proteins, Notch1, Immune regulation, Interleukin-2 Receptor alpha Subunit, Cell Differentiation, Forkhead Transcription Factors, Mesenchymal Stem Cells, T-Lymphocytes, Regulatory, Coculture Techniques, Gene Expression Regulation, T-Lymphocyte Subsets, CD4 Antigens, Basic Helix-Loop-Helix Transcription Factors, Humans, Transcription Factor HES-1, Immune regulation, Mesenchymal stem cells, Notch1, Tolerance, Treg cells, Lymphocyte Count, Receptor, Notch1, Antibodies, Blocking, Tolerance, Oligopeptides, Treg cells, Cells, Cultured, Signal Transduction
Abstract

Notch1 signaling is involved in regulatory T (Treg)-cell differentiation. We previously demonstrated that, when cocultured with CD3(+) cells, mesenchymal stem cells (MSCs) induced a T-cell population with a regulatory phenotype. Here, we investigated the molecular mechanism underlying MSC induction of human Treg cells. We show that the Notch1 pathway is activated in CD4(+) T cells cocultured with MSCs. Inhibition of Notch1 signaling through GSI-I or the Notch1 neutralizing antibody reduced expression of HES1 (the Notch1 downstream target) and the percentage of MSC-induced CD4(+) CD25(high) FOXP3(+) cells in vitro. Moreover, we demonstrate that FOXP3 is a downstream target of Notch signaling in human cells. No crosstalk between Notch1 and TGF-β signaling pathways was observed in our experimental system. Together, these findings indicate that activation of the Notch1 pathway is a novel mechanism in the human Treg-cell induction mediated by MSCs.

Published
2012

41. Macrophage apoptosis induced by Group B Streptococcus

Author

Fettucciari, Katia, Rosati, Emanuela, Scaringi, Lucia, Cornacchione, P., Migliorati, Graziella, Sabatini, R., Fetriconi, I., Rossi, Ruggero, and Marconi, Pierfrancesco

Subjects
macrofagi, streptococco gruppo b, apoptosi
Published
1999

47. γ‐Secretase inhibitor I induces apoptosis in chronic lymphocytic leukemia cells by proteasome inhibition, endoplasmic reticulum stress increase and notch down‐regulation

Author

Rosati, Emanuela, primary, Sabatini, Rita, additional, De Falco, Filomena, additional, Del Papa, Beatrice, additional, Falzetti, Franca, additional, Di Ianni, Mauro, additional, Cavalli, Laura, additional, Fettucciari, Katia, additional, Bartoli, Andrea, additional, Screpanti, Isabella, additional, and Marconi, Pierfrancesco, additional

Published
2012
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