11 results on '"Roopin M"'
Search Results
2. Computational based design and tracking of synthetic variants of Porcine circovirus reveal relations between silent genomic information and viral fitness.
- Author
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Baron L, Atar S, Zur H, Roopin M, Goz E, and Tuller T
- Subjects
- Animals, Cell Line, Circoviridae Infections virology, Entropy, Gene Library, Swine, Thermodynamics, Circovirus genetics, Computational Biology methods, Genome, Viral, Mutation genetics
- Abstract
Viral genomes not only code the protein content, but also include silent, overlapping codes which are important to the regulation of the viral life cycle and affect its evolution. Due to the high density of these codes, their non-modular nature and the complex intracellular processes they encode, the ability of current approaches to decipher them is very limited. We describe the first computational-experimental pipeline for studying the effects of viral silent and non-silent information on its fitness. The pipeline was implemented to study the Porcine Circovirus type 2 (PCV2), the shortest known eukaryotic virus, and includes the following steps: (1) Based on the analyses of 2100 variants of PCV, suspected silent codes were inferred. (2) Five hundred variants of the PCV2 were designed to include various 'smart' silent mutations. (3) Using state of the art synthetic biology approaches, the genomes of these five hundred variants were generated. (4) Competition experiments between the variants were performed in Porcine kidney-15 (PK15) cell-lines. (5) The variant titers were analyzed based on novel next-generation sequencing (NGS) experiments. (6) The features related to the titer of the variants were inferred and their analyses enabled detection of various novel silent functional sequence and structural motifs. Furthermore, we demonstrate that 50 of the silent variants exhibit higher fitness than the wildtype in the analyzed conditions.
- Published
- 2021
- Full Text
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3. Evolutionary selection against short nucleotide sequences in viruses and their related hosts.
- Author
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Zarai Y, Zafrir Z, Siridechadilok B, Suphatrakul A, Roopin M, Julander J, and Tuller T
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- Animals, Bacteria genetics, Bacteria virology, Female, Fungi genetics, Fungi virology, Host-Pathogen Interactions, Male, Mice, Oligonucleotides genetics, Plants genetics, Plants virology, Systems Biology methods, Zika Virus genetics, Zika Virus pathogenicity, Biological Coevolution, Evolution, Molecular, Genome, Viral, Nucleotide Motifs, Selection, Genetic
- Abstract
Viruses are under constant evolutionary pressure to effectively interact with the host intracellular factors, while evading its immune system. Understanding how viruses co-evolve with their hosts is a fundamental topic in molecular evolution and may also aid in developing novel viral based applications such as vaccines, oncologic therapies, and anti-bacterial treatments. Here, based on a novel statistical framework and a large-scale genomic analysis of 2,625 viruses from all classes infecting 439 host organisms from all kingdoms of life, we identify short nucleotide sequences that are under-represented in the coding regions of viruses and their hosts. These sequences cannot be explained by the coding regions' amino acid content, codon, and dinucleotide frequencies. We specifically show that short homooligonucleotide and palindromic sequences tend to be under-represented in many viruses probably due to their effect on gene expression regulation and the interaction with the host immune system. In addition, we show that more sequences tend to be under-represented in dsDNA viruses than in other viral groups. Finally, we demonstrate, based on in vitro and in vivo experiments, how under-represented sequences can be used to attenuated Zika virus strains., (© The Author(s) 2020. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.)
- Published
- 2020
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4. Possible cooption of a VEGF-driven tubulogenesis program for biomineralization in echinoderms.
- Author
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Morgulis M, Gildor T, Roopin M, Sher N, Malik A, Lalzar M, Dines M, Ben-Tabou de-Leon S, Khalaily L, and Ben-Tabou de-Leon S
- Subjects
- Animals, Calcium metabolism, Gene Regulatory Networks, Humans, Neovascularization, Physiologic, Receptors, Vascular Endothelial Growth Factor metabolism, Sea Urchins classification, Sea Urchins genetics, Signal Transduction, Vascular Endothelial Growth Factor A genetics, Vascular Endothelial Growth Factor A metabolism, Biomineralization, Sea Urchins growth & development, Vascular Endothelial Growth Factor A physiology
- Abstract
Biomineralization is the process by which living organisms use minerals to form hard structures that protect and support them. Biomineralization is believed to have evolved rapidly and independently in different phyla utilizing preexisting components. The mechanistic understanding of the regulatory networks that drive biomineralization and their evolution is far from clear. Sea urchin skeletogenesis is an excellent model system for studying both gene regulation and mineral uptake and deposition. The sea urchin calcite spicules are formed within a tubular cavity generated by the skeletogenic cells controlled by vascular endothelial growth factor (VEGF) signaling. The VEGF pathway is essential for biomineralization in echinoderms, while in many other phyla, across metazoans, it controls tubulogenesis and vascularization. Despite the critical role of VEGF signaling in sea urchin spiculogenesis, the downstream program it activates was largely unknown. Here we study the cellular and molecular machinery activated by the VEGF pathway during sea urchin spiculogenesis and reveal multiple parallels to the regulation of vertebrate vascularization. Human VEGF rescues sea urchin VEGF knockdown, vesicle deposition into an internal cavity plays a significant role in both systems, and sea urchin VEGF signaling activates hundreds of genes, including biomineralization and interestingly, vascularization genes. Moreover, five upstream transcription factors and three signaling genes that drive spiculogenesis are homologous to vertebrate factors that control vascularization. Overall, our findings suggest that sea urchin spiculogenesis and vertebrate vascularization diverged from a common ancestral tubulogenesis program, broadly adapted for vascularization and specifically coopted for biomineralization in the echinoderm phylum., Competing Interests: The authors declare no conflict of interest.
- Published
- 2019
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5. A global database of nitrogen and phosphorus excretion rates of aquatic animals.
- Author
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Vanni MJ, McIntyre PB, Allen D, Arnott DL, Benstead JP, Berg DJ, Brabrand Å, Brosse S, Bukaveckas PA, Caliman A, Capps KA, Carneiro LS, Chadwick NE, Christian AD, Clarke A, Conroy JD, Cross WF, Culver DA, Dalton CM, Devine JA, Domine LM, Evans-White MA, Faafeng BA, Flecker AS, Gido KB, Godinot C, Guariento RD, Haertel-Borer S, Hall RO, Henry R, Herwig BR, Hicks BJ, Higgins KA, Hood JM, Hopton ME, Ikeda T, James WF, Jansen HM, Johnson CR, Koch BJ, Lamberti GA, Lessard-Pilon S, Maerz JC, Mather ME, McManamay RA, Milanovich JR, Morgan DKJ, Moslemi JM, Naddafi R, Nilssen JP, Pagano M, Pilati A, Post DM, Roopin M, Rugenski AT, Schaus MH, Shostell J, Small GE, Solomon CT, Sterrett SC, Strand Ø, Tarvainen M, Taylor JM, Torres-Gerald LE, Turner CB, Urabe J, Uye SI, Ventelä AM, Villeger S, Whiles MR, Wilhelm FM, Wilson HF, Xenopoulos MA, and Zimmer KD
- Subjects
- Animals, Ecosystem, Fresh Water, Phylogeny, Aquatic Organisms metabolism, Nitrogen metabolism, Phosphorus metabolism
- Abstract
Animals can be important in modulating ecosystem-level nutrient cycling, although their importance varies greatly among species and ecosystems. Nutrient cycling rates of individual animals represent valuable data for testing the predictions of important frameworks such as the Metabolic Theory of Ecology (MTE) and ecological stoichiometry (ES). They also represent an important set of functional traits that may reflect both environmental and phylogenetic influences. Over the past two decades, studies of animal-mediated nutrient cycling have increased dramatically, especially in aquatic ecosystems. Here we present a global compilation of aquatic animal nutrient excretion rates. The dataset includes 10,534 observations from freshwater and marine animals of N and/or P excretion rates. These observations represent 491 species, including most aquatic phyla. Coverage varies greatly among phyla and other taxonomic levels. The dataset includes information on animal body size, ambient temperature, taxonomic affiliations, and animal body N:P. This data set was used to test predictions of MTE and ES, as described in Vanni and McIntyre (2016; Ecology DOI: 10.1002/ecy.1582)., (© 2017 by the Ecological Society of America.)
- Published
- 2017
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6. Dissecting common and divergent molecular pathways elicited by CdSe/ZnS quantum dots in freshwater and marine sentinel invertebrates.
- Author
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Ambrosone A, Roopin M, Pelaz B, Abdelmonem AM, Ackermann LM, Mattera L, Allocca M, Tino A, Klapper M, Parak WJ, Levy O, and Tortiglione C
- Subjects
- Animals, Anthozoa genetics, Anthozoa metabolism, Cadmium Compounds chemistry, Colloids, Endocytosis drug effects, Gene Expression Profiling, Hydra genetics, Hydra metabolism, Quantum Dots chemistry, Selenium Compounds chemistry, Sequence Analysis, RNA, Transcriptome drug effects, Zinc Compounds chemistry, Anthozoa drug effects, Cadmium Compounds toxicity, Fresh Water chemistry, Hydra drug effects, Quantum Dots toxicity, Selenium Compounds toxicity, Zinc Compounds toxicity
- Abstract
Water ecosystems represent main targets of unintentional contamination of nanomaterials, due to industrial waste or other anthropogenic activities. Nanoparticle insult to living organisms may occur in a sequential way, first by chemical interactions of the material with the target membrane, then by progressive internalisation and interaction with cellular structures and organelles. These events trigger a signal transduction, through which cells modulate molecular pathway in order to respond and survive to the external elicitation. Therefore, the analysis of the global changes of the molecular machinery, possibly induced in an organism upon exposure to a given nanomaterial, may provide unique clues for proper and exhaustive risk assessment. Here, we tested the impact of core/shell CdSe/ZnS QDs coated by a positively charged polymer on two aquatic species, the polyp Hydra vulgaris and the coral S. pistillata, representative of freshwater and sea habitats, respectively. By using reliable approaches based on animal behaviour and physiology together with a whole transcriptomic profiling, we determined several toxicity endpoints. Despite the difference in the efficiency of uptake, both species were severely affected by QD treatment, resulting in dramatic morphological damages and tissue bleaching. Global transcriptional changes were also detected in both organisms, but presenting different temporal dynamics, suggesting both common and divergent functional responses in the two sentinel organisms. Due to the striking conservation of structure and genomic organisation among animals throughout evolution, our expression profiling offers new clues to identify novel molecular markers and pathways for comparative transcriptomics of nanotoxicity.
- Published
- 2017
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7. Impact of Amorphous SiO2 Nanoparticles on a Living Organism: Morphological, Behavioral, and Molecular Biology Implications.
- Author
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Ambrosone A, Scotto di Vettimo MR, Malvindi MA, Roopin M, Levy O, Marchesano V, Pompa PP, Tortiglione C, and Tino A
- Abstract
It is generally accepted that silica (SiO2) is not toxic. But the increasing use of silica nanoparticles (SiO2NPs) in many different industrial fields has prompted the careful investigation of their toxicity in biological systems. In this report, we describe the effects elicited by SiO2NPs on animal and cell physiology. Stable and monodisperse amorphous silica nanoparticles, 25 nM in diameter, were administered to living Hydra vulgaris (Cnidaria). The dose-related effects were defined by morphological and behavioral assays. The results revealed an all-or-nothing lethal toxicity with a rather high threshold (35 nM NPs) and a LT50 of 38 h. At sub lethal doses, the morphophysiological effects included: animal morphology alterations, paralysis of the gastric region, disorganization and depletion of tentacle specialized cells, increase of apoptotic and collapsed cells, and reduction of the epithelial cell proliferation rate. Transcriptome analysis (RNAseq) revealed 45 differentially expressed genes, mostly involved in stress response and cuticle renovation. Our results show that Hydra reacts to SiO2NPs, is able to rebalance the animal homeostasis up to a relatively high doses of SiO2NPs, and that the physiological modifications are transduced to gene expression modulation.
- Published
- 2014
- Full Text
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8. Occurrence, diel patterns, and the influence of melatonin on the photosynthetic performance of cultured Symbiodinium.
- Author
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Roopin M, Yacobi YZ, and Levy O
- Subjects
- Chlorophyll metabolism, Chlorophyll A, Chromatography, High Pressure Liquid, Circadian Rhythm, Dinoflagellida metabolism, Dinoflagellida physiology, Melatonin analysis, Dinoflagellida drug effects, Melatonin pharmacology, Photosynthesis drug effects
- Abstract
Dinoflagellata is the earliest phylum in which true circadian regulation of melatonin rhythms has been convincingly demonstrated. Here, diel profiling of melatonin in a cultured member of this phylum belonging to the genus Symbiodinium indicated that melatonin levels oscillate with significant nocturnal peaks. However, unlike in other previously studied dinoflagellate species, the diel rhythmicity of melatonin in Symbiodinium did not persist under constant dark conditions. Thus, the oscillating pattern of melatonin in Symbiodinium is presumed not to be driven by endogenous circadian control of melatonin production, but rather by changes in the daily photocycle, most likely through a mechanism involving the enhanced photo-consumption of melatonin by free radicals. Although direct interactions of melatonin with detrimental radicals have been previously studied in several basal species, including dinoflagellates, none of these investigations addressed the effects that this molecule may have on photosynthesis, a major source of radical species in unicellular algae. In the present work, real-time monitoring of oxygen evolution in Symbiodinium cultures indicated a significant decrease in photosynthesis rates upon treatment with various doses of melatonin. Analyses of chlorophyll a fluorescence and xanthophyll cycle activity confirmed this effect and further revealed that this slowdown may occur through an enhanced engagement of photoprotective mechanisms in melatonin-treated cells. These findings are of great importance as they demonstrate that in certain photoautotroph species, the interactions of elevated melatonin levels with photosynthesis may extend beyond the general purpose of antioxidant protection., (© 2013 John Wiley & Sons A/S. Published by Blackwell Publishing Ltd.)
- Published
- 2013
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9. Photosynthetic circadian rhythmicity patterns of Symbiodinium, [corrected] the coral endosymbiotic algae.
- Author
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Sorek M, Yacobi YZ, Roopin M, Berman-Frank I, and Levy O
- Subjects
- Algal Proteins genetics, Algal Proteins metabolism, Animals, Carotenoids metabolism, Chlorophyll metabolism, Chromatography, High Pressure Liquid, Circadian Rhythm, DNA, Complementary genetics, DNA, Complementary metabolism, Molecular Sequence Data, Oxygen metabolism, Photoperiod, Photosystem II Protein Complex metabolism, Polymerase Chain Reaction, RNA, Messenger genetics, RNA, Messenger metabolism, Sequence Analysis, DNA, Anthozoa physiology, Dinoflagellida physiology, Photosynthesis, Symbiosis
- Abstract
Biological clocks are self-sustained endogenous timers that enable organisms (from cyanobacteria to humans) to anticipate daily environmental rhythms, and adjust their physiology and behaviour accordingly. Symbiotic corals play a central role in the creation of biologically rich ecosystems based on mutualistic symbioses between the invertebrate coral and dinoflagellate protists from the genus Symbiodinium. In this study, we experimentally establish that Symbiodinium photosynthesis, both as a free-living unicellular algae and as part of the symbiotic association with the coral Stylophora pistillata, is 'wired' to the circadian clock mechanism with a 'free-run' cycle close to 24 h. Associated photosynthetic pigments also showed rhythmicity under light/dark conditions and under constant light conditions, while the expression of the oxygen-evolving enhancer 1 gene (within photosystem II) coincided with photosynthetically evolved oxygen in Symbiodinium cultures. Thus, circadian regulation of the Symbiodinium photosynthesis is, however, complicated as being linked to the coral/host that have probably profound physiochemical influence on the intracellular environment. The temporal patterns of photosynthesis demonstrated here highlight the physiological complexity and interdependence of the algae circadian clock associated in this symbiosis and the plasticity of algae regulatory mechanisms downstream of the circadian clock.
- Published
- 2013
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10. Temporal and histological evaluation of melatonin patterns in a 'basal' metazoan.
- Author
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Roopin M and Levy O
- Subjects
- Animals, Biological Evolution, Darkness, FMRFamide analysis, Photoperiod, Sea Anemones anatomy & histology, Sea Anemones chemistry, Melatonin physiology, Sea Anemones physiology
- Abstract
While recent advances suggest functional pleiotropy of melatonin in higher organisms, an understanding of the biological significance of this ancient molecule in early evolutionary groups is lacking. Here, endogenous melatonin production was identified for the first time in the sea anemone Actinia equina, a nonsymbiotic hexacorallian cnidarian. Day/night activity profiles of melatonin in this anemone indicated that melatonin levels oscillate with significant nocturnal peaks. However, dynamic changes in melatonin concentration did not persist under constant dark conditions and therefore were not circadian in nature. Thus, the oscillating pattern of melatonin in A. equina is presumed to be the result of alternative, simpler melatonin control mechanism that likely involves direct regulation by the daily photocycle. As nocturnal melatonin signals still potentially provide 'time-of-day' information and can illustrate the seasonally changing length of the biological night, we hypothesize that melatonin may be relevant to temporal coordination of timed processes also in anthozoans. Spatial patterns of melatonin distribution found in this study indicate abundant melatonin distribution in the endodermal filaments wrapped around gametes. This finding supports the possibility that one of the melatonin-responsive processes in this basal metazoan species may involve reproductive functions., (© 2012 John Wiley & Sons A/S.)
- Published
- 2012
- Full Text
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11. Melatonin distribution reveals clues to its biological significance in basal metazoans.
- Author
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Roopin M and Levy O
- Subjects
- Animals, Gene Expression Regulation, Melatonin biosynthesis, Melatonin isolation & purification, Nerve Net metabolism, Protein Transport, Receptors, Melatonin metabolism, Reproduction, Sea Anemones physiology, Evolution, Molecular, Melatonin metabolism, Sea Anemones metabolism
- Abstract
Although nearly ubiquitous in nature, the precise biological significance of endogenous melatonin is poorly understood in phylogenetically basal taxa. In the present work, we describe insights into the functional role of melatonin at the most "basal" level of metazoan evolution. Hitherto unknown morphological determinants of melatonin distribution were evaluated in Nematostella vectensis by detecting melatonin immunoreactivity and examining the spatial gene expression patterns of putative melatonin biosynthetic and receptor elements that are located at opposing ends of the melatonin signaling pathway. Immuno-melatonin profiling indicated an elaborate interaction with reproductive tissues, reinforcing previous conjectures of a melatonin-responsive component in anthozoan reproduction. In situ hybridization (ISH) to putative melatonin receptor elements highlighted the possibility that the bioregulatory effects of melatonin in anthozoan reproduction may be mediated by interactions with membrane receptors, as in higher vertebrates. Another intriguing finding of the present study pertains to the prevalence of melatonin in centralized nervous structures. This pattern may be of great significance given that it 1) identifies an ancestral association between melatonin and key neuronal components and 2) potentially implies that certain effects of melatonin in basal species may be spread widely by regionalized nerve centers.
- Published
- 2012
- Full Text
- View/download PDF
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