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14. The Effect of Marginal Zn2+Excess Released from Titanium Coating on Differentiation of Human Osteoblastic Cells

Author

Bartmański, Michał, Pawłowski, Łukasz, Knabe, Agata, Mania, Szymon, Banach-Kopeć, Adrianna, Sakowicz-Burkiewicz, Monika, and Ronowska, Anna

Abstract

Composite coatings based on chitosan and zinc nanoparticles (ZnNPs) were successfully produced on Ti13Zr13Nb substrates by cathodic electrophoretic deposition (EPD). The unfavorable phenomenon of water electrolysis-induced nonuniformity was reduced by applying a low voltage (20 V) and a short deposition time (1 min). Surface analysis (roughness and hydrophilicity) reveals the potential of these coatings for enhancing cell attachment and bone-implant integration. However, there is a concern about adhesion and strength; therefore, incorporating ZnNPs shows promise for enhancing mechanical properties, suggesting opportunities for further optimization of the process. The aim of this work was to investigate whether Zn2+released from coating yields overt cellular impairment. hFOB1.19 osteoblastic cells were used as a model in this study. A subtoxic, 0.125 mmol/L, Zn concentration did not cause significant negative changes in cultured osteoblastic cells, as there was no significant change in their viability and their mitochondrial metabolism. Moreover, the alkaline phosphatase and lactate dehydrogenase activities were aggravated. However, a high, over 0.175 mmol/L, Zn2+concentration caused total cell death. This was caused by the inhibition of mitochondrial enzymes’ activities. Our data indicate that composite coatings releasing Zn2+may be used as the differentiating factor toward osteoblastic cells.

Published
2024
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18. Cytocompatibility, antibacterial, and corrosion properties of chitosan/polymethacrylates and chitosan/poly(4‐vinylpyridine) smart coatings, electrophoretically deposited on nanosilver‐decorated titania nanotubes

Author

Pawłowski, Łukasz, primary, Bartmański, Michał, additional, Ronowska, Anna, additional, Banach‐Kopeć, Adrianna, additional, Mania, Szymon, additional, Cieślik, Bartłomiej Michał, additional, Mielewczyk‐Gryń, Aleksandra, additional, Karczewski, Jakub, additional, and Zieliński, Andrzej, additional

Published
2023
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20. Osteoblast and bacterial cell response on RGD peptide‐functionalized chitosan coatings electrophoretically deposited from different suspensions on Ti13Nb13Zr alloy

Author

Pawłowski, Łukasz, primary, Mania, Szymon, additional, Banach‐Kopeć, Adrianna, additional, Bartmański, Michał, additional, Ronowska, Anna, additional, Jurak, Kacper, additional, Mielewczyk‐Gryń, Aleksandra, additional, Karska, Natalia, additional, Rodziewicz‐Motowidło, Sylwia, additional, and Zieliński, Andrzej, additional

Published
2023
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22. Cytocompatibility, antibacterial, and corrosion properties of chitosan/polymethacrylates and chitosan/poly(4‐vinylpyridine) smart coatings, electrophoretically deposited on nanosilver‐decorated titania nanotubes.

Author

Pawłowski, Łukasz, Bartmański, Michał, Ronowska, Anna, Banach‐Kopeć, Adrianna, Mania, Szymon, Cieślik, Bartłomiej Michał, Mielewczyk‐Gryń, Aleksandra, Karczewski, Jakub, and Zieliński, Andrzej

Subjects
POLYMETHACRYLATES, NANOTUBES, CHITOSAN, CONTACT angle, ESCHERICHIA coli
Abstract

The development of novel implants subjected to surface modification to achieve high osteointegration properties at simultaneous antimicrobial activity is a highly current problem. This study involved different surface treatments of titanium surface, mainly by electrochemical oxidation to produce a nanotubular oxide layer (TNTs), a subsequent electrochemical reduction of silver nitrate and decoration of a nanotubular surface with silver nanoparticles (AgNPs), and finally electrophoretic deposition (EPD) of a composite of chitosan (CS) and either polymethacrylate‐based copolymer Eudragit E 100 (EE100) or poly(4‐vinylpyridine) (P4VP) coating. The effects of each stage of this multi‐step modification were examined in terms of morphology, roughness, wettability, corrosion resistance, coating‐substrate adhesion, antibacterial properties, and osteoblast cell adhesion and proliferation. The results showed that the titanium surface formed nanotubes (inner diameter of 97 ± 12 nm, length of 342 ± 36 nm) subsequently covered with silver nanoparticles (with a diameter of 88 ± 8 nm). Further, the silver‐decorated nanotubes were tightly coated with biopolymer films. Most of the applied modifications increased both the roughness and the surface contact angle of the samples. The deposition of biopolymer coatings resulted in reduced burst release of silver. The coated samples revealed potent antimicrobial activity against both Gram‐positive and Gram‐negative bacteria. Total elimination (99.9%) of E. coli was recorded for a sample with CS/P4VP coating. Cytotoxicity results using hFOB 1.19, a human osteoblast cell line, showed that after 3 days the tested modifications did not affect the cellular growth according to the titanium control. The proposed innovative multilayer antibacterial coatings can be successful for titanium implants as effective postoperative anti‐inflammation protection. [ABSTRACT FROM AUTHOR]

Published
2024
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23. Evaluating Gelatin-Based Films with Graphene Nanoparticles for Wound Healing Applications.

Author

Kamedulski, Piotr, Wekwejt, Marcin, Zasada, Lidia, Ronowska, Anna, Michno, Anna, Chmielniak, Dorota, Binkowski, Paweł, Łukaszewicz, Jerzy P., and Kaczmarek-Szczepańska, Beata

Subjects
GRAPHENE, NANOPARTICLES, YOUNG'S modulus, RAMAN spectroscopy, SELF-healing materials, WATER vapor, HEALING, WOUND healing
Abstract

In this study, gelatin-based films containing graphene nanoparticles were obtained. Nanoparticles were taken from four chosen commercial graphene nanoplatelets with different surface areas, such as 150 m2/g, 300 m2/g, 500 m2/g, and 750 m2/g, obtained in different conditions. Their morphology was observed using SEM with STEM mode; porosity, Raman spectra and elemental analysis were checked; and biological properties, such as hemolysis and cytotoxicity, were evaluated. Then, the selected biocompatible nanoparticles were used as the gelatin film modification with 10% concentration. As a result of solvent evaporation, homogeneous thin films were obtained. The surface's properties, mechanical strength, antioxidant activity, and water vapor permeation rate were examined to select the appropriate film for biomedical applications. We found that the addition of graphene nanoplatelets had a significant effect on the properties of materials, improving surface roughness, surface free energy, antioxidant activity, tensile strength, and Young's modulus. For the most favorable candidate for wound dressing applications, we chose a gelatin film containing nanoparticles with a surface area of 500 m2/g. [ABSTRACT FROM AUTHOR]

Published
2023
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45. Aggravated effects of coexisting marginal thiamine deficits and zinc excess on SN56 neuronal cells.

Author

Ronowska, Anna, Gul-Hinc, Sylwia, Michno, Anna, Bizon-Zygmańska, Dorota, Zyśk, Marlena, Bielarczyk, Hanna, Szutowicz, Andrzej, Gapys, Beata, and Jankowska-Kulawy, Agnieszka

Subjects
*THIAMIN pyrophosphate, *VITAMIN B1, *VITAMIN B deficiency, *ZINC, *ENERGY metabolism, *ALZHEIMER'S disease, *BRAIN diseases
Abstract

Objectives: Zinc excitotoxicity and thiamine pyrophosphate deficiency (TD) are known pathogenic signals contributing to mechanism of different encephalopathies through inhibition of enzymes responsible for energy metabolism such as pyruvate dehydrogenase, aconitase or ketoglutarate dehydrogenase. The aim of this work was to investigate whether subclinical Zn excess and TD, frequent in aging brain, may combine yielding overt neuronal impairment. Results: Clonal SN56 cholinergic neuronal cells of septal origin were used as the model of brain cholinergic neurons, which are particularly susceptible to neurodegeneration in the course of Alzheimer's disease, hypoxia and other dementia-linked brain pathologies. Neither subtoxic concentration of Zn (0.10 mM) nor mild 20–25% TD deficits alone caused significant negative changes in cultured cholinergic neurons viability and their acetyl-CoA/acetylcholine metabolism. However, cells with mild TD accumulated Zn in excess, which impaired their energy metabolism causing a loss of neurons viability and their function as neurotransmitters. These negative effects of Zn were aggravated by amprolium which is an inhibitor of thiamine intracellular transport. Conclusion: Our data indicate that TD may amplify otherwise non-harmful border-line Zn excitotoxic signals yielding progress of neurodegeneration. [ABSTRACT FROM AUTHOR]

Published
2021
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50. Protective effects of voltage-gated calcium channel antagonists against zinc toxicity in SN56 neuroblastoma cholinergic cells

Author

Zysk, Marlena, Gapys, Beata, Ronowska, Anna, Gul-Hinc, Sylwia, Erlandsson, Anna, Iwanicki, Adam, Sakowicz-Burkiewicz, Monika, Szutowicz, Andrzej, Bielarczyk, Hanna, Zysk, Marlena, Gapys, Beata, Ronowska, Anna, Gul-Hinc, Sylwia, Erlandsson, Anna, Iwanicki, Adam, Sakowicz-Burkiewicz, Monika, Szutowicz, Andrzej, and Bielarczyk, Hanna

Abstract

One of the pathological site effects in excitotoxic activation is Zn2+ overload to postsynaptic neurons. Such an effect is considered to be equivalent to the glutamate component of excitotoxicity. Excessive uptake of Zn2+ by active voltage-dependent transport systems in these neurons may lead to significant neurotoxicity. The aim of this study was to investigate whether and which antagonists of the voltage gated calcium channels (VGCC) might modify this Zn2+-induced neurotoxicity in neuronal cells. Our data demonstrates that depolarized SN56 neuronal cells may take up large amounts of Zn2+ and store these in cytoplasmic and mitochondrial sub-fractions. The mitochondrial Zn2+ excess suppressed pyruvate uptake and oxidation. Such suppression was caused by inhibition of pyruvate dehydrogenase complex, aconitase and NADP-isocitrate dehydrogenase activities, resulting in the yielding of acetyl-CoA and ATP shortages. Moreover, incoming Zn2+ increased both oxidized glutathione and malondialdehyde levels, known parameters of oxidative stress. In depolarized SN56 cells, nifedipine treatment (L-type VGCC antagonist) reduced Zn2+ uptake and oxidative stress. The treatment applied prevented the activities of PDHC, aconitase and NADP-IDH enzymes, and also yielded the maintenance of acetyl-CoA and ATP levels. Apart from suppression of oxidative stress, N- and P/Q-type VGCCs presented a similar, but weaker protective influence. In conclusion, our data shows that in the course of excitotoxity, impairment to calcium homeostasis is tightly linked with an excessive neuronal Zn2+ uptake. Hence, the VGCCs types L, N and P/Q share responsibility for neuronal Zn2+ overload followed by significant energy-dependent neurotoxicity. Moreover, Zn2+ affects the target tricarboxylic acid cycle enzymes, yields acetyl-CoA and energy deficits as well.

Published
2018
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