1. Oxidative stress coping capacity (OSC) value: Development and validation of an in vitro measurement method for blood plasma using electron paramagnetic resonance spectroscopy (EPR) and vitamin C
- Author
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Pauline Nibbe, Johannes Schleusener, Silas Siebert, Richard Borgart, Doreen Brandt, Ronja Westphalen, Nadine Schüler, Bastian Berger, Eva M.J. Peters, Martina C. Meinke, and Silke B. Lohan
- Subjects
Oxidative Stress ,Plasma ,Physiology (medical) ,Electron Spin Resonance Spectroscopy ,Humans ,Pilot Projects ,Ascorbic Acid ,Vitamins ,Oxidation-Reduction ,Biochemistry ,Antioxidants - Abstract
Oxidative stress as a driver of disease is reinforcing the trend towards supplementation with antioxidants. While antioxidants positively influence the redox status when applied at physiological doses, higher concentrations may have pro-oxidative effects. Precise assessment methods for testing the supply of antioxidants are lacking. Using in-situ-irradiation as stressor and electron paramagnetic resonance (EPR) spectroscopy as readout system for formed radicals, a stress response assessment method was developed, using protein solutions and plasma samples from transfusion medicine. The method was validated in a double-blind placebo-controlled in vivo cross-over pilot study in blood plasma samples of individuals before and after vitamin C supplementation. Reference measurements were performed for the exogenous antioxidants β-carotene and vitamin C, and glutathione as an endogenous representative. Malondialdehyde was studied for oxidative stress indication. Protein solutions without antioxidants showed a linear increase in radical concentration during irradiation. The in-vitro-addition of vitamin C or plasma samples from subjects displayed two slopes (m
- Published
- 2023
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