1. Hemoglobin Biosynthesis in Murine Virus-induced Leukemic Cells In Vitro: Structure and Amounts of Globin Chains Produced
- Author
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Charlotte Friend, William Scher, Rondall Young, Samuel H. Boyer, Andrea N. Noyes, Harvey D. Preisler, Kuang Dong Wuu, and Arthur Bank
- Subjects
Radial immunodiffusion ,chemistry.chemical_classification ,Messenger RNA ,Immunology ,Cell ,Cell Biology ,Hematology ,Biology ,Biochemistry ,Molecular biology ,In vitro ,Amino acid ,medicine.anatomical_structure ,chemistry ,Cell culture ,medicine ,Hemoglobin ,Globin - Abstract
Tryptic peptides characteristic of mouse hemoglobin α- and diffuse-type β-chains were isolated from dimethylsulfoxide-treated mouse leukemia virus-infected cell cultures. Quantitative estimates of in vitro hemoglobin synthesis by such cultures were obtained by two different methods: by analysis of radioactive amino acid incorporation, and by single-cell radial immunodiffusion assay. The amounts produced, approximately 4 pg/cell/17 hr, reflect a synthetic capacity that is probably sufficient for 32P labeling of hemoglobin messenger RNAs (mRNA) in quantities commensurate with structural characterization of mRNAs by radioautographic methods.
- Published
- 1972
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