8 results on '"Rompf J"'
Search Results
2. Annals of internal medicine: A case-management system for coronary risk factor modification after acute myocardial infarction
- Author
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Debusk, R. F., Miller, N. H., H. Robert Superko, Dennis, C. A., Thomas, R. J., Lew, H. T., Berger Iii, W. E., Heller, R. S., Rompf, J., Gee, D., Kraemer, H. C., Bandura, A., Ghandour, G., Clark, M., Shah, R. V., Fisher, L., and Taylor, C. B.
3. MOVEMENTS OF THE BASE OF THE VENTRICLE AND THE RELATIVE CONSTANCY OF THE CARDIAC VOLUME
- Author
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Hamilton, W. F., primary and Rompf, J. H., additional
- Published
- 1932
- Full Text
- View/download PDF
4. Plasma procalcitonin kinetics in healthy dogs and dogs undergoing tibial plateau leveling osteotomy.
- Author
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Rompf J, Hettlich B, Lutz B, Marti E, Mirkovitch J, Peters L, Adamik KN, Schüpbach-Regula G, Willi B, and Schuller S
- Subjects
- Dogs, Humans, Animals, Anterior Cruciate Ligament surgery, Tibia surgery, Procalcitonin, Longitudinal Studies, Prospective Studies, Stifle, Osteotomy veterinary, Osteotomy methods, C-Reactive Protein, Retrospective Studies, Anterior Cruciate Ligament Injuries surgery, Anterior Cruciate Ligament Injuries veterinary, Dog Diseases surgery
- Abstract
Background: Procalcitonin (PCT) is a well-established biomarker for bacterial infection in human patients., Objectives: We aimed to analyze the kinetics of plasma PCT (pPCT) in healthy dogs and dogs with canine cranial cruciate ligament (CCL) rupture undergoing tibial plateau leveling osteotomy (TPLO)., Methods: This prospective, longitudinal study included 15 healthy dogs and 25 dogs undergoing TPLO. Hematology, pPCT, and C-reactive protein (CRP) were assessed on 3 consecutive days in healthy dogs and 1 day preoperatively and days 1, 2, 10, and 56 postoperatively. Inter- and intraindividual variability of pPCT were assessed in healthy dogs. Median pPCT concentrations of dogs with CCL rupture preoperatively were compared with healthy controls, and median pPCT concentrations, as well as percentage change post anesthesia, arthroscopy, and TPLO, were compared with baseline. For the correlation analysis, the Spearman rank correlation test was used., Results: Inter- and intraindividual variabilities of pPCT in healthy dogs were 36% and 15%, respectively. Median baseline pPCT concentrations were not significantly different between healthy dogs (118.9 pg/mL; IQR: 75.3-157.3 pg/mL) and dogs undergoing TPLO (95.9 pg/mL; IQR: 63.8-117.0 pg/mL). Plasma PCT concentrations were significantly lower immediately post- than preoperatively (P < 0.001). CRP, WBC, and neutrophil concentrations increased significantly on post-OP day 2 and had normalized by day 10., Conclusions: These results indicate that CCL rupture, as well as anesthesia, arthroscopy, and TPLO combined, are not associated with increased pPCT concentrations in dogs with uncomplicated recovery. Considering the high intraindividual variability, individual serial measurements rather than a population-based reference interval should be considered., (© 2023 The Authors. Veterinary Clinical Pathology published by Wiley Periodicals LLC on behalf of American Society for Veterinary Clinical Pathology.)
- Published
- 2023
- Full Text
- View/download PDF
5. Fragmentation of the CRISPR-Cas Type I-B signature protein Cas8b.
- Author
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Richter H, Rompf J, Wiegel J, Rau K, and Randau L
- Subjects
- Amino Acid Sequence, Archaeal Proteins genetics, Archaeal Proteins metabolism, Bacterial Proteins genetics, Bacterial Proteins metabolism, CRISPR-Associated Proteins metabolism, Cloning, Molecular, Clostridium thermocellum genetics, Clostridium thermocellum metabolism, Clustered Regularly Interspaced Short Palindromic Repeats genetics, Methanococcus genetics, RNA Processing, Post-Transcriptional, Recombinant Fusion Proteins metabolism, Signal Transduction genetics, CRISPR-Associated Proteins genetics, CRISPR-Cas Systems genetics, Recombinant Fusion Proteins genetics
- Abstract
Background: CRISPR arrays are transcribed into long precursor RNA species, which are further processed into mature CRISPR RNAs (crRNAs). Cas proteins utilize these crRNAs, which contain spacer sequences that can be derived from mobile genetic elements, to mediate immunity during a reoccurring virus infection. Type I CRISPR-Cas systems are defined by the presence of different Cascade interference complexes containing large and small subunits that play major roles during target DNA selection., Methods: Here, we produce the protein and crRNA components of the Type I-B CRISPR-Cas complex of Clostridium thermocellum and Methanococcus maripaludis. The C. thermocellum Cascade complexes were reconstituted and analyzed via size-exclusion chromatography. Activity of the heterologous M. maripaludis CRISPR-Cas system was followed using phage lambda plaques assays., Results: The reconstituted Type-I-B Cascade complex contains Cas7, Cas5, Cas6b and the large subunit Cas8b. Cas6b can be omitted from the reconstitution protocol. The large subunit Cas8b was found to be represented by two tightly associated protein fragments and a small C-terminal Cas8b segment was identified in recombinant complexes and C. thermocellum cell lysate., Conclusions: Production of Cas8b generates a small C-terminal fragment, which is suggested to fulfill the role of the missing small subunit. A heterologous, synthetic M. maripaludis Type I-B system is active in E. coli against phage lambda, highlighting a potential for genome editing using endogenous Type-I-B CRISPR-Cas machineries. This article is part of a Special Issue entitled "Biochemistry of Synthetic Biology - Recent Developments" Guest Editor: Dr. Ilka Heinemann and Dr. Patrick O'Donoghue., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
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6. Alginate-based hydrogels with improved adhesive properties for cell encapsulation.
- Author
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Sarker B, Rompf J, Silva R, Lang N, Detsch R, Kaschta J, Fabry B, and Boccaccini AR
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- Biocompatible Materials chemistry, Cell Line, Cell Proliferation, Gelatin chemistry, Glucuronic Acid chemistry, Hexuronic Acids chemistry, Humans, Mechanical Phenomena, Mitochondria metabolism, Osteoblasts metabolism, Alginates chemistry, Hydrogels chemistry, Tissue Adhesives chemistry
- Abstract
Hydrogel-based biomaterials are ideal scaffolding matrices for microencapsulation, but they need to be modified to resemble the mechanical, structural and chemical properties of the native extracellular matrix. Here, we compare the mechanical properties and the degradation behavior of unmodified and modified alginate hydrogels in which cell adhesive functionality is conferred either by blending or covalently cross-linking with gelatin. Furthermore, we measure the spreading and proliferation of encapsulated osteoblast-like MG-63 cells. Alginate hydrogels covalently crosslinked with gelatin show the highest degree of cell adhesion, spreading, migration, and proliferation, as well as a faster degradation rate, and are therefore a particularly suitable material for microencapsulation., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
7. A case-management system for coronary risk factor modification after acute myocardial infarction.
- Author
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DeBusk RF, Miller NH, Superko HR, Dennis CA, Thomas RJ, Lew HT, Berger WE 3rd, Heller RS, Rompf J, Gee D, Kraemer HC, Bandura A, Ghandour G, Clark M, Shah RV, Fisher L, and Taylor CB
- Subjects
- Adult, Aged, Cholesterol, LDL blood, Confounding Factors, Epidemiologic, Counseling, Female, Humans, Hypercholesterolemia complications, Hypercholesterolemia diet therapy, Male, Middle Aged, Myocardial Infarction blood, Myocardial Infarction etiology, Nutritional Physiological Phenomena, Patient Care Team, Risk Factors, San Francisco, Smoking adverse effects, Treatment Outcome, Exercise Therapy, Hypercholesterolemia drug therapy, Managed Care Programs, Myocardial Infarction rehabilitation, Smoking Cessation
- Abstract
Objective: To evaluate the efficacy of a physician-directed, nurse-managed, home-based case-management system for coronary risk factor modification., Design: Randomized clinical trial in which patients received a special intervention (n = 293) or usual medical care (n = 292) during the first year after acute myocardial infarction., Setting: 5 Kaiser Permanente Medical Centers in the San Francisco Bay area., Patients: 585 men and women aged 70 years or younger who were hospitalized for acute myocardial infarction., Intervention: In the hospital, specially trained nurses initiated interventions for smoking cessation, exercise training, and diet-drug therapy for hyperlipidemia. Intervention after discharge was implemented primarily by telephone and mail contact with patients in their homes. All medically eligible patients received exercise training; all smokers received the smoking cessation intervention; and all patients received dietary counseling and, if needed, lipid-lowering drug therapy., Outcome: Smoking prevalence and plasma low-density lipoprotein cholesterol (LDL) concentrations were measured 2 months after infarction, and functional capacity was measured 6 months after infarction., Results: In the special intervention and usual care groups, the cotinine-confirmed smoking cessation rates were 70% and 53% (P = 0.03), plasma LDL cholesterol levels were 2.77 +/- 0.69 mmol/L and 3.41 +/- 0.90 mmol/L (107 +/- 30 mg/dL and 132 +/- 30 mg/dL) (P = 0.001), and functional capacities were 9.3 +/- 2.4 METS and 8.4 +/- 2.5 METS (P = 0.001), respectively., Conclusion: In a large health maintenance organization, a case-management system was considerably more effective than usual medical care for modification of coronary risk factors after myocardial infarction.
- Published
- 1994
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8. Conversion hysteria.
- Author
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ROMPF JH
- Subjects
- Humans, Conversion Disorder, Neurotic Disorders
- Published
- 1948
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