1. A comprehensive analysis of the genetic diversity and environmental adaptability in worldwide Merino and Merino-derived sheep breeds
- Author
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Simone Ceccobelli, Vincenzo Landi, Gabriele Senczuk, Salvatore Mastrangelo, Maria Teresa Sardina, Slim Ben-Jemaa, Christian Persichilli, Taki Karsli, Valentin-Adrian Bâlteanu, María Agustina Raschia, Mario Andrés Poli, Gabriel Ciappesoni, Farai Catherine Muchadeyi, Edgar Farai Dzomba, Nokuthula Winfred Kunene, Gesine Lühken, Tatiana Evgenievna Deniskova, Arsen Vladimirovich Dotsev, Natalia Anatolievna Zinovieva, Attila Zsolnai, István Anton, Szilvia Kusza, Nuno Carolino, Fátima Santos-Silva, Aldona Kawęcka, Marcin Świątek, Roman Niżnikowski, Marija Špehar, Gabriel Anaya, Antonio Granero, Tiago Perloiro, Pedro Cardoso, Silverio Grande, Beatriz López de los Santos, Coralie Danchin-Burge, Marina Pasquini, Amparo Martínez Martínez, Juan Vicente Delgado Bermejo, Emiliano Lasagna, Elena Ciani, Francesca Maria Sarti, and Fabio Pilla
- Subjects
Animal culture ,SF1-1100 ,Genetics ,QH426-470 - Abstract
Abstract Background To enhance and extend the knowledge about the global historical and phylogenetic relationships between Merino and Merino-derived breeds, 19 populations were genotyped with the OvineSNP50 BeadChip specifically for this study, while an additional 23 populations from the publicly available genotypes were retrieved. Three complementary statistical tests, Rsb (extended haplotype homozygosity between-populations), XP-EHH (cross-population extended haplotype homozygosity), and runs of homozygosity (ROH) islands were applied to identify genomic variants with potential impact on the adaptability of Merino genetic type in two contrasting climate zones. Results The results indicate that a large part of the Merino’s genetic relatedness and admixture patterns are explained by their genetic background and/or geographic origin, followed by local admixture. Multi-dimensional scaling, Neighbor-Net, Admixture, and TREEMIX analyses consistently provided evidence of the role of Australian, Rambouillet and German strains in the extensive gene introgression into the other Merino and Merino-derived breeds. The close relationship between Iberian Merinos and other South-western European breeds is consistent with the Iberian origin of the Merino genetic type, with traces from previous contributions of other Mediterranean stocks. Using Rsb and XP-EHH approaches, signatures of selection were detected spanning four genomic regions located on Ovis aries chromosomes (OAR) 1, 6 and 16, whereas two genomic regions on OAR6, that partially overlapped with the previous ones, were highlighted by ROH islands. Overall, the three approaches identified 106 candidate genes putatively under selection. Among them, genes related to immune response were identified via the gene interaction network. In addition, several candidate genes were found, such as LEKR1, LCORL, GHR, RBPJ, BMPR1B, PPARGC1A, and PRKAA1, related to morphological, growth and reproductive traits, adaptive thermogenesis, and hypoxia responses. Conclusions To the best of our knowledge, this is the first comprehensive dataset that includes most of the Merino and Merino-derived sheep breeds raised in different regions of the world. The results provide an in-depth picture of the genetic makeup of the current Merino and Merino-derived breeds, highlighting the possible selection pressures associated with the combined effect of anthropic and environmental factors. The study underlines the importance of Merino genetic types as invaluable resources of possible adaptive diversity in the context of the occurring climate changes.
- Published
- 2023
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