1. Grape ASR Regulates Glucose Transport, Metabolism and Signaling
- Author
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Parrilla, Jonathan, Medici, Anna, Gaillard, Cécile, Verbeke, Jérémy, Gibon, Yves, Rolin, Dominique, Laloi, Maryse, Finkelstein, Ruth R, and Atanassova, Rossitza
- Subjects
Biochemistry and Cell Biology ,Biological Sciences ,Nutrition ,Abscisic Acid ,Arabidopsis ,Arabidopsis Proteins ,Gene Expression Regulation ,Plant ,Glucose ,Plant Proteins ,Signal Transduction ,Vitis ,ASR ,VvMSA overexpression ,repression ,VvHT1 ,grape embryogenic and non-embryogenic cells ,glucose signaling ,glucose absorption ,sugar transporters ,central metabolism ,Arabidopsis abi8 mutant ,ABA ,glucose sensitivity ,germination ,ABA/glucose sensitivity ,VvMSA overexpression/repression ,Other Chemical Sciences ,Genetics ,Other Biological Sciences ,Chemical Physics ,Biochemistry and cell biology ,Microbiology ,Medicinal and biomolecular chemistry - Abstract
To decipher the mediator role of the grape Abscisic acid, Stress, Ripening (ASR) protein, VvMSA, in the pathways of glucose signaling through the regulation of its target, the promoter of hexose transporter VvHT1, we overexpressed and repressed VvMSA in embryogenic and non-embryogenic grapevine cells. The embryogenic cells with organized cell proliferation were chosen as an appropriate model for high sensitivity to the glucose signal, due to their very low intracellular glucose content and low glycolysis flux. In contrast, the non-embryogenic cells displaying anarchic cell proliferation, supported by high glycolysis flux and a partial switch to fermentation, appeared particularly sensitive to inhibitors of glucose metabolism. By using different glucose analogs to discriminate between distinct pathways of glucose signal transduction, we revealed VvMSA positioning as a transcriptional regulator of the glucose transporter gene VvHT1 in glycolysis-dependent glucose signaling. The effects of both the overexpression and repression of VvMSA on glucose transport and metabolism via glycolysis were analyzed, and the results demonstrated its role as a mediator in the interplay of glucose metabolism, transport and signaling. The overexpression of VvMSA in the Arabidopsis mutant abi8 provided evidence for its partial functional complementation by improving glucose absorption activity.
- Published
- 2022