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The New World screwworm, Cochliomyia hominivorax Coquerel (Diptera: Calliphoridae), was officially eliminated from Costa Rica in 2000, but it was reintroduced in 2023. A myiasis by C. hominivorax in a 71-year-old man with a 4-month history of foot hyperkeratosis and interdigital ulcers is reported. The myiasis was detected before sampling for bacterial culture. Approximately 160 first- and second-instar larvae were recovered and identified as C. hominivorax. Morphological identification was based mainly on characteristics of the cephalopharyngeal skeleton, spiracles, and pigmented dorsal tracheal trunks. Sequencing of a cytochrome c oxidase subunit I gene fragment confirmed the identity. The ulcers healed after extraction of the larvae and ciprofloxacin treatment of a concurrent Staphylococcus aureus and Pseudomonas aeruginosa infection. Given the reintroduction of C. hominivorax in Costa Rica and the risk of northward expansion, this report highlights its impact on public health and calls for awareness among clinicians and healthcare practitioners.

Throughout history, women have been actively involved in the advancement of science, while struggling to overcome challenges to participate and a lack of recognition. Prior to 1950, most women were not included in the lists of "classical" descriptions of the iconic scientific figures nor included in the most relevant historical accounts. Since the second half of the twentieth century, great efforts have been made to recognize the contributions of women to the advancement of science, especially since formal scientific careers have been dominated by men, with limited (or no) access to women. Despite these challenging social, political and cultural contexts, many women have succeeded in making significant advancements, and their contributions are now being acknowledged. Such efforts have led to the publication of recent reviews and compilations on outstanding women in biological sciences. The field of medical entomology is inherently interdisciplinary, focusing on insects and other arthropods that affect human health, with input primarily from the biological and medical sciences and a strong public health perspective. Several reviews and book chapters describing the history of medical entomology have been published over the decades, but few women are mentioned in these publications, even though many women have contributed to this field. Much of the information on these women is currently scattered throughout the published literature and historical records on a wide range of topics, including activism, virology, vector control and even acarology. Considering that there is no single available compilation of women contributors in the history of medical entomology, this review aims to provide a list of 22 women and their contributions to this field. The list includes women from diverse backgrounds, born in the late 1800s and before 1950, who directly impacted medical entomology in various ways and in different regions of the world. This compilation is far from exhaustive, but it aims to identify role models and examples of extraordinary women to motivate the evolving future of this field., (© 2022. The Author(s).)

Five different fluorescent powders (orange, yellow, green, blue, and violet) were tested on Aedes aegypti adults to evaluate the differentiation of multiple fluorescent powder colors applied externally in the same female mosquito, their effect on coupling time, copulation time, insemination success, mate choice, and the extent of transference of powders between marked and unmarked individuals, either during copulation or same-sex interactions. Marking with multiple powders was evaluated after applying different powders in the same female at different times and combinations. The comparative effect of powders on mating was explored using different cross-combinations of marked/unmarked couples. Transference of powders between marked/unmarked individuals after copulation was checked in mated individuals, and between same-sex interactions by allowing them to interact under crowded and uncrowded conditions. Identification of the colors included in multiple markings in the same individual was possible when exploring almost all combinations (exception: green-yellow). No important effect of powder marking between cross-combinations was found on coupling time (overall 95% CI (Confidence Interval) 37.6-49.6 min), copulation time (overall 95% CI 17-20 s), insemination success, nor their mate choice. Transferred powder after copulation activity, concentrated in genitalia, legs, and the tip of wings, occurred in >80% of females and 100% of males. Powder transference in legs and genitalia, between same-sex individuals, occurred only in males (ranged between 23-35%) under both density conditions. The lack of important effects of these powders on the studied aspects of Ae. aegypti provides information about their usefulness and limitations, which should be recognized for future applications and to avoid bias.

Fluorescent powders are one of the most common external markers used to study mosquito behavior and ecology. For their reliable and practical use, it is important to evaluate their effect on biological parameters such as survival, blood-feeding, and mobility. We evaluated the effect of five different fluorescent powders (Day-Glo ® ECO Series) on the survival of different age cohorts, blood-feeding success, and tethered flight speed on Aedes aegypti (Linnaeus) adult females. For survival analysis, three cohorts (2-5, 6-9 and 10-13 days old) were marked and mortality recorded until all died. To examine the effect of fluorescent powders on female response to blood-feeding, the proportions of unfed, partially fed, and fully engorged females, after being exposed to host blood under two different time sets (20 and 40 min.), were compared. Their impact on female tethered flight speed was evaluated recording their flight for 30 min. with a flight mill. Survival distributions between treatments were not significantly different within each cohort. Blood-feeding was not significantly different among marked or unmarked females at both times of blood exposure, with the exception of Signal Green-ECO 18 and Ultra Violet- ECO 20 (at 20 and 40 min.), in which a higher proportion of partially fed females was observed compared to control females. In relation to flight performance, no statistically significant difference in mean tethered flight speed (m/s), among marked and unmarked mosquito groups, was observed. Our results indicate that the tested powders and application method have few significant impacts on Ae. aegypti survival, blood-feeding success and flight performance, and are thus suitable for investigations of mosquito biology in the environment., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

The use of insect markers, such as fluorescent powders, is a useful tool for studying ecological and epidemiological questions. Evaluating their effect on vectors of human disease agents, such as the invasive mosquito vector Aedes aegypti (Linnaeus), is crucial for their practical and reliable use, especially in parameters linked to the risk of disease transmission such as adult survival, dispersal, and host-seeking. Seven fluorescent powders (Hercules Radiant, DayGlo (DG), Risk Reactor (RR), and Angstrom Technologies), applied externally on cohorts of Ae. aegypti female mosquitoes, were tested to determine their impact on survival and recapture by baited mosquito traps, and their detectability after being exposed to controlled laboratory and semifield environments. There were no significant differences in survival among marked and unmarked females across all powders. Marked females were significantly less likely to be captured in baited traps relative to unmarked females, except for one of the DG powders. All females remained visibly marked on five parts of their body for 30 d (under both environments), except for one of the RR powders. The tested powders and application method are suitable for tracking mosquitoes throughout most of their lives under different environments, without significantly affecting their survival, but with potential impact on recapture by baited traps, possibly due to effects on senses or other physiological traits., (© The Author(s) 2019. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)