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BackgroundPer- and polyfluoroalkyl substances (PFASs) have been detected in drinking water supplies around the world and are the subject of intense regulatory debate. While they have been associated with several illnesses, their effects on reproductive outcomes remains uncertain.MethodsWe analyzed birth outcomes in the east Minneapolis-St. Paul metropolitan area from 2002 to 2011, where a portion of the population faced elevated exposure to PFASs due to long-term contamination of drinking water supplies from industrial waste disposal. Installation of a water filtration facility in the highly contaminated city of Oakdale, MN at the end of 2006 resulted in a sharp decrease in exposure to PFASs, creating a "natural experiment". Using a difference-in-differences approach, we compare the changes in birth outcomes before and after water filtration in Oakdale to the changes over the same period in neighboring communities where the treatment of municipal water remained constant.ResultsAverage birth weight and average gestational age were statistically significantly lower in the highly exposed population than in the control area prior to filtration of municipal water supply. The highly exposed population faced increased odds of low birth weight (adjusted odds ratio 1.36, 95% CI 1.25-1.48) and pre-term birth (adjusted odds ratio 1.14, 95% CI 1.09-1.19) relative to the control before filtration, and these differences moderated after filtration. The general fertility rate was also significantly lower in the exposed population (incidence rate ratio 0.73, 95% CI 0.69-0.77) prior to filtration and appeared to be rebounding post-2006.ConclusionsOur findings provide evidence of a causal relationship between filtration of drinking water containing high levels of exposure to PFASs and improved reproductive outcomes.

The kynurenine pathway (KP) is the principal route of L-Tryptophan (TRP) catabolism leading to the production of kynurenine (KYN), the neuroprotectants, kynurenic acid (KYNA) and picolinic acid (PIC), and the excitotoxic neurotoxin, quinolinic acid (QUIN). The enzymes indoleamine 2,3-dioxygenase-1 (IDO-1), indoleamine 2, 3-dioxygenase-2 (IDO-2) and tryptophan 2,3-dioxygenase (TDO-2) initiate the first step of the KP. Downstream enzymes include kynureninase (KYNU), 3-hydroxyanthranilate 3,4-dioxygenase (3-HAAO), kynurenine hydroxylase (KMO) and 2-amino-3-carboxymuconate semialdehyde decarboxylase (ACMSD). Kynurenine aminotransferase-I (KAT-I) is one of the enzymes responsible for synthesising KYNA. Mounting evidence directly implicates that IDO-1 induction in various tumours is a crucial mechanism facilitating tumour immune evasion and persistence. However, the involvement of the downstream machinery of the KP in brain tumour progression remains unexplored. A complete characterisation of the KP in brain tumours and the role of the KP in maintaining homeostasis between neuroprotection and neurodegeneration in glioma has not yet been investigated. Here we report the first comprehensive characterisation of the KP in cultured human glioma cells and GBM patient plasma. Our qRT-PCR data revealed that interferon-gamma (IFN-γ) (100 IU/ml) stimulation significantly potentiated the expression of IDO-1 IDO-2, KYNU, 3-HAAO, KMO and significantly down-regulated ACMSD and KAT-I expression in cultured human glioma cells. HPLC analysis revealed that IFN-γ stimulation significantly increased KP activity (KYN/TRP ratio), and significantly lowered the KYNA/KYN neuroprotective ratio in human cultured glioma cells. Our HPLC and GCMS data revealed that KP activation was significantly higher and the concentrations of TRP, KYNA, QUIN and PIC and the KYNA/KYN ratio were significantly lower in GBM patient plasma (n = 18) compared to controls. These results provide further evidence for the involvement of the KP in glioma pathophysiology and highlights a potential role of KP products as novel and highly attractive therapeutic targets to evaluate for the treatment of brain tumours.

Discusses use of the World Wide Web in education and its possibilities for developing higher order critical thinking skills to successfully deal with the demands of the future information society. Suggests that teachers need to provide learning environments that are learner-centered, authentic, problem-based, and collaborative. (Contains 61 references.) (Author/LRW)

Some solutions are offered for three large-class testing problems: how to offer students an opportunity to be assessed in an essay format without straining the available grading resources; deal with students who miss a required examination; and generate large numbers of new, relevant examination questions regularly. (MSE)

ABSTRACT If nursing, along with other health professions, is to be able to critique national and international health policy and be equipped to address the global and planetary dimensions of [...]

Statement Validity Assessment (SVA) is a comprehensive credibility assessment system, with the Criterion-Based Content Analysis (CBCA) as a core component. Worldwide, the CBCA is reported to be the most widely used veracity assessment instrument. We tested and confirmed the hypothesis that CBCA scores are affected by event familiarity; descriptions of familiar events are more likely to be judged true than are descriptions of unfamiliar events. CBCA scores were applied to transcripts of 114 children who recalled a routine medical procedure (control) or a traumatic medical procedure that they had experienced one time (relatively unfamiliar) or multiple times (relatively familiar). CBCA scores were higher for children in the relatively familiar than the relatively unfamiliar condition, and CBCA scores were significantly correlated with age. Results raise serious questions regarding the forensic suitability of the CBCA for assessing the veracity of children's accounts.

Companies are increasingly focused on driving the adoption of nature‐based solutions across their organizations. Yet, implementing nature‐based solutions within existing regulatory frameworks poses a unique set of challenges. In this paper, we present three nature‐based solution case studies from The Dow Chemical Company and The Nature Conservancy's nearly 10‐year collaboration. In the first case study, we focus on the potential benefits of reforestation to support the state's air quality improvement efforts. Ultimately, federal and state authorities did not approve of the reforestation project. Following this early setback, the collaboration team developed a suite of science‐based tools that could be used to better advocate for government approval for the implementation of nature‐based solutions. In the second case study, we highlight how one of these tools, the Ecosystem Services Identification & Inventory Tool, was used to improve communications about the benefits of nature‐based solutions with regulatory agencies. In this case, Dow ultimately received approval for the restoration of a wetland to remediate an existing ash pond. Finally, the third case study highlights how engaging the right expertise through collaboration between the private sector and conservationists can improve land management strategies. Overall, this paper emphasizes the importance of robust conservation science, tools and expertise, and thoughtful collaboration as necessary means of driving the adoption of nature‐based solutions both within a company and by its regulating entities. Integr Environ Assess Manag 2022;18:74–81. © 2021 SETAC KEY POINTS: This paper emphasizes the importance of robust conservation science, tools and expertise, and thoughtful collaboration as necessary means of driving the adoption of nature‐based solutions both within a company and by their regulating entities. [ABSTRACT FROM AUTHOR]