11 results on '"Roenhorst, Johanna W."'
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2. Dahlia Latent Viroid
- Author
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Verhoeven, Jacobus Th.J., primary, Meekes, Ellis T.M., additional, Roenhorst, Johanna W., additional, Flores, Ricardo, additional, and Serra, Pedro, additional
- Published
- 2017
- Full Text
- View/download PDF
3. List of Contributors
- Author
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Alazem, Mazen, primary, Al-Tuwariqi, Hani Z., additional, Barba, Marina, additional, Boubourakas, Iraklis N., additional, Çağlayan, Kadriye, additional, Candresse, Thierry, additional, Conejero, Vicente, additional, Dadami, Elena, additional, Dalakouras, Athanasios, additional, Daròs, José-Antonio, additional, De la Peña, Marcos, additional, Delgado, Sonia, additional, Di Serio, Francesco, additional, Dixon, Jonathan, additional, Duran-Vila, Nuria, additional, El-Dougdoug, Khaled A., additional, Elleuch, Amine, additional, Faggioli, Francesco, additional, Flores, Ricardo, additional, Fraile, Aurora, additional, Gago-Zachert, Selma, additional, Gallitelli, Donato, additional, García-Arenal, Fernando, additional, Geering, Andrew D.W., additional, Giunchedi, Luciano, additional, Gómez, Gustavo, additional, Gyamera, Ebenezer A., additional, Habili, Nuredin, additional, Hadidi, Ahmed, additional, Hallan, Vipin, additional, Hammond, Rosemarie W., additional, Hanold, Dagmar, additional, Hataya, Tatsuji, additional, Hernández, Carmen, additional, Hong, Ni, additional, Hosakawa, Munetaka, additional, Hsu, Yau-Heiu, additional, Hu, Chung-Chi, additional, Huang, Ying-Wen, additional, James, Delano, additional, Joseph, Hendry, additional, Kalantidis, Kriton, additional, Kaponi, Maria S., additional, Kim, Mikyeong, additional, Kinard, Gary R., additional, Kong, Lih L., additional, Kovalskaya, Natalia, additional, Kuhn, David N., additional, Kyriakopoulou, Panayota E., additional, Lavagi, Irene, additional, Li, Shi-Fang, additional, Lin, Na-Sheng, additional, Ling, Kai-Shu, additional, Liou, Ming-Ru, additional, Lisón, Purificación, additional, López-Carrasco, Amparo, additional, Luigi, Marta, additional, Martínez de Alba, Ángel-Emilio, additional, Mascia, Tiziana, additional, Masuta, Chikara, additional, Matoušek, Jaroslav, additional, Maurel, Marie-Christine, additional, Meekes, Ellis T.M., additional, Minoia, Sofia, additional, Navarro, Beatriz, additional, Nie, Xianzhou, additional, Owens, Robert A., additional, Pallás, Vicente, additional, Palukaitis, Peter, additional, Parakh, Dattaraj B., additional, Perreault, Jean-Pierre, additional, Podleckis, Edward V., additional, Pyle, Jesse D., additional, Randles, John W., additional, Riesner, Detlev, additional, Rodriguez, M. Judith B., additional, Roenhorst, Johanna W., additional, Roossinck, Marilyn J., additional, Rubino, Luisa, additional, Sánchez-Navarro, Jesus A., additional, Sano, Teruo, additional, Scholthof, Karen-Beth G., additional, Serra, Pedro, additional, Shimura, Hanako, additional, Singh, Rudra P., additional, Škorić, Dijana, additional, Stancanelli, Giuseppe, additional, Steger, Gerhard, additional, Taglienti, Anna, additional, Tessitori, Matilde, additional, Thanarajoo, Sathis S., additional, Tiberini, Antonio, additional, Torchetti, Enza M., additional, Tsagris, Mina, additional, Tsushima, Taro, additional, Vadamalai, Ganesan, additional, Verhoeven, Jacobus Th.J., additional, Vidalakis, Georgios, additional, Wang, Qiao-Chun, additional, Wassenegger, Michael, additional, Winter, Stephan, additional, Xu, Wen-Xing, additional, Yang, Xiuling, additional, Zhang, Yongjiang, additional, Zhang, Zhibo, additional, Zhou, Xueping, additional, and Zhu, Shuifang, additional
- Published
- 2017
- Full Text
- View/download PDF
4. Facilitating the adoption of high‐throughput sequencing technologies as a plant pest diagnostic test in laboratories: A step‐by‐step description
- Author
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Lebas, Benedicte, primary, Adams, Ian, additional, Al Rwahnih, Maher, additional, Baeyen, Steve, additional, Bilodeau, Guillaume J., additional, Blouin, Arnaud G., additional, Boonham, Neil, additional, Candresse, Thierry, additional, Chandelier, Anne, additional, De Jonghe, Kris, additional, Fox, Adrian, additional, Gaafar, Yahya Z. A., additional, Gentit, Pascal, additional, Haegeman, Annelies, additional, Ho, Wellcome, additional, Hurtado‐Gonzales, Oscar, additional, Jonkers, Wilfried, additional, Kreuze, Jan, additional, Kutjnak, Denis, additional, Landa, Blanca, additional, Liu, Mingxin, additional, Maclot, François, additional, Malapi‐Wight, Martha, additional, Maree, Hano J., additional, Martoni, Francesco, additional, Mehle, Natasha, additional, Minafra, Angelantonio, additional, Mollov, Dimitre, additional, Moreira, Adriana, additional, Nakhla, Mark, additional, Petter, Françoise, additional, Piper, Alexander M., additional, Ponchart, Julien, additional, Rae, Robbie, additional, Remenant, Benoit, additional, Rivera, Yazmin, additional, Rodoni, Brendan, additional, Roenhorst, Johanna W., additional, Rollin, Johan, additional, Saldarelli, Pasquale, additional, Santala, Johanna, additional, Souza‐Richards, Rose, additional, Spadaro, Davide, additional, Studholme, David J., additional, Sultmanis, Stefanie, additional, van der Vlugt, René, additional, Tamisier, Lucie, additional, Trontin, Charlotte, additional, Vazquez‐Iglesias, Ines, additional, Vicente, Claudia S. L., additional, Vossenberg, Bart T. L. H., additional, Wetzel, Thierry, additional, Ziebell, Heiko, additional, and Massart, Sebastien, additional
- Published
- 2022
- Full Text
- View/download PDF
5. Facilitating the adoption of high-throughput sequencing technologies as a plant pest diagnostic test in laboratories: A step-by-step description
- Author
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European Commission, Lebas, Benedicte, Adams, Ian, Al Rwahnih, Maher, Baeyen, Steve, Bilodeau, Guillaume J., Blouin, Arnaud G., Boonham, Neil, Candresse, Thierry, Chandelier, Anne, De Jonghe, Kris, Fox, Adrian, Gaafar, Yahya Z. A., Gentit, Pascal, Haegeman, Annelies, Ho, Wellcome, Hurtado-Gonzales, Oscar, Jonkers, Wilfried, Kreuze, Jan, Kutjnak, Denis, Landa, Blanca B., Liu, Mingxin, Maclot, François, Malapi-Wight, Martha, Maree, Hano J., Martoni, Francesco, Mehle, Natasha, Minafra, Angelantonio, Mollov, Dimitre, Moreira, Adriana, Nakhla, Mark, Petter, Françoise, Piper, Alexander M., Ponchart, Julien, Rae, Robbie, Remenant, Benoit, Rivera, Yazmin, Rodoni, Brendan, Roenhorst, Johanna W, Rollin, Johan, Saldarelli, Pasquale, Santala, Johanna, Souza-Richards, Rose, Spadaro, Davide, Studholme, David J., Sultmanis, Stefanie, van der Vlugt, René, Tamisier, Lucie, Trontin, Charlotte, Vazquez-Iglesias, Ines, Vicente, Claudia S.L., Vossenberg, Bart T. L. H., Wetzel, Thierry, Ziebell, Heiko, Massart, Sebastien, European Commission, Lebas, Benedicte, Adams, Ian, Al Rwahnih, Maher, Baeyen, Steve, Bilodeau, Guillaume J., Blouin, Arnaud G., Boonham, Neil, Candresse, Thierry, Chandelier, Anne, De Jonghe, Kris, Fox, Adrian, Gaafar, Yahya Z. A., Gentit, Pascal, Haegeman, Annelies, Ho, Wellcome, Hurtado-Gonzales, Oscar, Jonkers, Wilfried, Kreuze, Jan, Kutjnak, Denis, Landa, Blanca B., Liu, Mingxin, Maclot, François, Malapi-Wight, Martha, Maree, Hano J., Martoni, Francesco, Mehle, Natasha, Minafra, Angelantonio, Mollov, Dimitre, Moreira, Adriana, Nakhla, Mark, Petter, Françoise, Piper, Alexander M., Ponchart, Julien, Rae, Robbie, Remenant, Benoit, Rivera, Yazmin, Rodoni, Brendan, Roenhorst, Johanna W, Rollin, Johan, Saldarelli, Pasquale, Santala, Johanna, Souza-Richards, Rose, Spadaro, Davide, Studholme, David J., Sultmanis, Stefanie, van der Vlugt, René, Tamisier, Lucie, Trontin, Charlotte, Vazquez-Iglesias, Ines, Vicente, Claudia S.L., Vossenberg, Bart T. L. H., Wetzel, Thierry, Ziebell, Heiko, and Massart, Sebastien
- Abstract
High-throughput sequencing (HTS) is a powerful tool that enables the simultaneous detection and potential identification of any organisms present in a sample. The growing interest in the application of HTS technologies for routine diagnostics in plant health laboratories is triggering the development of guidelines on how to prepare laboratories for performing HTS testing. This paper describes general and technical recommendations to guide laboratories through the complex process of preparing a laboratory for HTS tests within existing quality assurance systems. From nucleic acid extractions to data analysis and interpretation, all of the steps are covered to ensure reliable and reproducible results. These guidelines are relevant for the detection and identification of any plant pest (e.g. arthropods, bacteria, fungi, nematodes, invasive plants or weeds, protozoa, viroids, viruses), and from any type of matrix (e.g. pure microbial culture, plant tissue, soil, water), regardless of the HTS technology (e.g. amplicon sequencing, shotgun sequencing) and of the application (e.g. surveillance programme, phytosanitary certification, quarantine, import control). These guidelines are written in general terms to facilitate the adoption of HTS technologies in plant pest routine diagnostics and enable broader application in all plant health fields, including research. A glossary of relevant terms is provided among the Supplementary Material.
- Published
- 2022
6. Facilitating the adoption of high-throughput sequencing technologies as a plant pest diagnostic test in laboratories : A step-by-step description
- Author
-
Lebas, Benedicte, Adams, Ian, Al Rwahnih, Maher, Baeyen, Steve, Bilodeau, Guillaume J., Blouin, Arnaud G., Boonham, Neil, Candresse, Thierry, Chandelier, Anne, De Jonghe, Kris, Fox, Adrian, Gaafar, Yahya Z.A., Gentit, Pascal, Haegeman, Annelies, Ho, Wellcome, Hurtado-Gonzales, Oscar, Jonkers, Wilfried, Kreuze, Jan, Kutjnak, Denis, Landa, Blanca, Liu, Mingxin, Maclot, François, Malapi-Wight, Martha, Maree, Hano J., Martoni, Francesco, Mehle, Natasha, Minafra, Angelantonio, Mollov, Dimitre, Moreira, Adriana, Nakhla, Mark, Petter, Françoise, Piper, Alexander M., Ponchart, Julien, Rae, Robbie, Remenant, Benoit, Rivera, Yazmin, Rodoni, Brendan, Roenhorst, Johanna W., Rollin, Johan, Saldarelli, Pasquale, Santala, Johanna, Souza-Richards, Rose, Spadaro, Davide, Studholme, David J., Sultmanis, Stefanie, van der Vlugt, René, Tamisier, Lucie, Trontin, Charlotte, Vazquez-Iglesias, Ines, Vicente, Claudia S.L., Vossenberg, Bart T.L.H., Wetzel, Thierry, Ziebell, Heiko, Massart, Sebastien, Lebas, Benedicte, Adams, Ian, Al Rwahnih, Maher, Baeyen, Steve, Bilodeau, Guillaume J., Blouin, Arnaud G., Boonham, Neil, Candresse, Thierry, Chandelier, Anne, De Jonghe, Kris, Fox, Adrian, Gaafar, Yahya Z.A., Gentit, Pascal, Haegeman, Annelies, Ho, Wellcome, Hurtado-Gonzales, Oscar, Jonkers, Wilfried, Kreuze, Jan, Kutjnak, Denis, Landa, Blanca, Liu, Mingxin, Maclot, François, Malapi-Wight, Martha, Maree, Hano J., Martoni, Francesco, Mehle, Natasha, Minafra, Angelantonio, Mollov, Dimitre, Moreira, Adriana, Nakhla, Mark, Petter, Françoise, Piper, Alexander M., Ponchart, Julien, Rae, Robbie, Remenant, Benoit, Rivera, Yazmin, Rodoni, Brendan, Roenhorst, Johanna W., Rollin, Johan, Saldarelli, Pasquale, Santala, Johanna, Souza-Richards, Rose, Spadaro, Davide, Studholme, David J., Sultmanis, Stefanie, van der Vlugt, René, Tamisier, Lucie, Trontin, Charlotte, Vazquez-Iglesias, Ines, Vicente, Claudia S.L., Vossenberg, Bart T.L.H., Wetzel, Thierry, Ziebell, Heiko, and Massart, Sebastien
- Abstract
High-throughput sequencing (HTS) is a powerful tool that enables the simultaneous detection and potential identification of any organisms present in a sample. The growing interest in the application of HTS technologies for routine diagnostics in plant health laboratories is triggering the development of guidelines on how to prepare laboratories for performing HTS testing. This paper describes general and technical recommendations to guide laboratories through the complex process of preparing a laboratory for HTS tests within existing quality assurance systems. From nucleic acid extractions to data analysis and interpretation, all of the steps are covered to ensure reliable and reproducible results. These guidelines are relevant for the detection and identification of any plant pest (e.g. arthropods, bacteria, fungi, nematodes, invasive plants or weeds, protozoa, viroids, viruses), and from any type of matrix (e.g. pure microbial culture, plant tissue, soil, water), regardless of the HTS technology (e.g. amplicon sequencing, shotgun sequencing) and of the application (e.g. surveillance programme, phytosanitary certification, quarantine, import control). These guidelines are written in general terms to facilitate the adoption of HTS technologies in plant pest routine diagnostics and enable broader application in all plant health fields, including research. A glossary of relevant terms is provided among the Supplementary Material.
- Published
- 2022
7. Possible Overestimation of Seed Transmission in the Spread of Pospiviroids in Commercial Pepper and Tomato Crops Based on Large-Scale Grow-Out Trials and Systematic Literature Review
- Author
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Verhoeven, Jacobus T. J., primary, Botermans, Marleen, additional, Schoen, Ruben, additional, Koenraadt, Harrie, additional, and Roenhorst, Johanna W., additional
- Published
- 2021
- Full Text
- View/download PDF
8. Development and validation of a real-time RT-PCR test for screening pepper and tomato seed lots for the presence of pospiviroids
- Author
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Botermans, Marleen, primary, Roenhorst, Johanna W., additional, Hooftman, Marinus, additional, Verhoeven, Jacobus Th. J., additional, Metz, Eveline, additional, van Veen, Esther J., additional, Geraats, Bart P. J., additional, Kemper, Mark, additional, Beugelsdijk, Debora C. M., additional, Koenraadt, Harrie, additional, Jodlowska, Agata, additional, and Westenberg, Marcel, additional
- Published
- 2020
- Full Text
- View/download PDF
9. Chapter 20 - Dahlia Latent Viroid
- Author
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Verhoeven, Jacobus Th.J., Meekes, Ellis T.M., Roenhorst, Johanna W., Flores, Ricardo, and Serra, Pedro
- Published
- 2017
- Full Text
- View/download PDF
10. Dahlia latent viroid: a recombinant new species of the family Pospiviroidae posing intriguing questions about its origin and classification
- Author
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Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes, Ministerio de Ciencia e Innovación, Verhoeven, Jacobus Th. J., Meekes, Ellis T. M., Roenhorst, Johanna W., Flores Pedauye, Ricardo, Serra Alfonso, Pedro, Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes, Ministerio de Ciencia e Innovación, Verhoeven, Jacobus Th. J., Meekes, Ellis T. M., Roenhorst, Johanna W., Flores Pedauye, Ricardo, and Serra Alfonso, Pedro
- Abstract
[EN] A viroid-like RNA has been detected in two asymptomatic dahlia accessions by return and double PAGE. It appeared smaller than Chrysanthemum stunt viroid and Potato spindle tuber viroid, the two members of the genus Pospiviroid, family Pospiviroidae, reported in this ornamental previously. RT-PCR with primers designed for amplifying all pospiviroids produced no amplicons, but RT-PCR with random primers revealed a 342 nt RNA. The sequence of this RNA was confirmed with specific primers, which additionally revealed its presence in many dahlia cultivars. The RNA was named Dahlia latent viroid (DLVd) because it replicates autonomously, but symptomlessly, in dahlia and shares maximum sequence identity with other viroids of less than 56 %. Furthermore, DLVd displays characteristic features of the family Pospiviroidae: a predicted rod-like secondary structure of minimum free energy with a central conserved region (CCR), and the ability to form the metastable structures hairpins I and II. Its CCR is identical to that of Hop stunt viroid (HSVd, genus Hostuviroid). However, DLVd: (i) has the terminal conserved region present in members of the genus Pospiviroid, but absent in HSVd, and (ii) lacks the terminal conserved hairpin present in HSVd. Phylogenetic reconstructions indicate that HSVd and Pepper chat fruit viroid (genus Pospiviroid) are the closest relatives of DLVd, but DLVd differs from these viroids in its host range, restricted to dahlia so far. Therefore, while DLVd fulfils the criteria to be a novel species of the family Pospiviroidae, its recombinant origin makes assignment to the genera Pospiviroid or Hostuviroid problematic
- Published
- 2013
11. Dahlia latent viroid: a recombinant new species of the family Pospiviroidae posing intriguing questions about its origin and classification
- Author
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Verhoeven, Jacobus Th. J., primary, Meekes, Ellis T. M., additional, Roenhorst, Johanna W., additional, Flores, Ricardo, additional, and Serra, Pedro, additional
- Published
- 2013
- Full Text
- View/download PDF
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