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Strip loins from two experiments were used to evaluate effects of feeding dry (DDG) or wet (WDG) distiller's grains on beef color, tenderness, and sensory traits of Holstein steers. In Exp. 1, conducted at the University of Illinois at Champaign--Urbana, dietary treatments consisted of a control whole corn--corn silage diet with soybean meal (SBM) or diets formulated with 12.5% DDG plus urea, 25% DDG, 25% WDG, 50% DDG, or 50% WDG (DM basis). In Exp. 2, conducted at Iowa State University, dietary treatments consisted of cracked corn--corn silage--hay diets with either SBM or urea (Urea) as the control diets, or diets formulated with 10, 20, or 40% DDG or WDG (DM basis). Within each study, strip loins from each of four steers (representing 45.7 and 66.6% of steers in Exp. 1 and 2, respectively) in four replicate pens per treatment were aged for 13 d at 4[degrees]C for subsequent color, tenderness, and palatability evaluation. Color of steaks was measured objectively using a HunterLab Miniscan XE spectrophotometer and was subjectively evaluated by a trained panel. Tenderness was measured using the Warner-Bratzler shear force (WBSF) instrument on steaks cooked to 70[degrees]C. For sensory evaluation, 95 consumers were recruited to evaluate tenderness, juiciness, and flavor of cooked steaks. In Exp. 1, steaks from steers fed 25% WDG had higher (P < 0.05) a* values after 138 h of simulated retail display than all other treatments, except for those from steers fed 12.5% DDG. In Exp. 2, a greater (P < 0.05) percentage of steaks from steers fed 40% DDG or 40% WDG were considered moderately undesirable during retail display (steaks that received a consumer acceptability score of 3 or less). There were no (P = 0.20 in Exp. 1, and P = 0.33 in Exp. 2) differences among treatments in Exp. 1 and Exp. 2 for WBSF (1.47 [+ or -] 0.66 kg and 1.58 [+ or -] 0.72 kg, respectively) or taste panel tenderness (5.7 [+ or -] 0.30 and 6.2 [+ or -] 0.22, respectively), beef flavor (6.0 [+ or -] 0.23 and 6.2 [+ or -] 0.22, respectively), and juiciness (5.6 [+ or -] 0.31 and 5.8 [+ or -] 0.23). Feeding distiller's grains at up to 50% of the dietary DM did not affect tenderness or sensory traits, and seems to be a viable feed alternative without negatively impacting sensory attributes. Key Words: Beef, Carcass, Distiller's Grains, Holstein, Sensory Traits

Concern has been raised about green discoloration of injection-site lesions in chuck muscles in modified-atmosphere packages. Objectives were: 1) to recreate green lesions, 2) to compare the severity of discoloration of injection-site lesions in chucks from carcasses of control or vitamin E-supplemented steers, and 3) to identify pigment(s) responsible for discoloration via in vitro color reactions. In Exp. 1, 23 steers (BW = 415 kg; 37 d before harvest) were injected with one of 12 pharmaceuticals, following label directions for route and dose, with the exception of a 5-mL maximum dose, to identify a product that could result in discoloration. Two vaccines (Products A and B) resulted in greening. In Exp. 2, 50 steers were injected (i.m.) with Product A and assigned to the control or vitamin E (1,000 IU/steer daily for 60 d) group. After retail display, 80 and 72% of steaks from the control and treatment groups, respectively, were discolored. Although vitamin E did not reduce (P = 0.53) greening, there was a trend (P = 0.10) toward delay discoloration of lesions from the treatment group. In Phase I of Exp. 3, pigments extracted from green lesions obtained from Exp. 2 were compared with solutions, exposed to a high partial pressure of oxygen (ppO), of myoglobin (Mb), copper sulfate, hydrogen peroxide ([H.sub.2][O.sub.2]), vaccine, and aluminum hydroxide either alone or in combination. In Phase II of Exp. 3, solutions of two or more of Mb, Cu, sodium sulfide, sodium sulfite, sodium sulfate ([Na.sub.2]S[O.sub.4]), and [H.sub.2][O.sub.2] were made at pH 7.2 or 5.5 and exposed to low or high ppO. Normal muscle tissue displayed a 3.2 and 56.7% decrease in absorbance/[micro]g of protein as wavelength changed from 654 to 656 nm and 656 to 658 nm, respectively. Pigments from control and treatment group green tissue displayed a 164.5 and 621.3% increase, respectively, in absorbance/[micro]g of protein as wavelength changed from 654 to 656 nm. As wavelength changed from 656 to 658 nm, the absorbance/ [micro]g of protein for control and treatment group lesions decreased by 75 and 109%, respectively. The Mb+Cu+[Na.sub.2]S[O.sub.4] solution, at pH 5.5 and high ppO, exhibited similar absorbance trends as green lesions indicating that greening may result from a Mb, Cu, and [Na.sub.2]S[O.sub.4] interaction. Results indicated that greening varies with pharmaceuticals and oxidation of tissue cannot be controlled with vitamin E supplementation. Research on the causative agents of green discoloration, with an emphasis on compounds containing sulfate or Cu, is needed. Key Words: Discoloration, Intramuscular Injection, Muscle Tissue, Subcutaneous Injection, Vitamin E

This study was conducted to determine the effectiveness of the SmartMV prototype BeefCam the effectiveness of the SmartMV prototype BeefCam Video Imaging System (prototype BeefCam) for classifying beef carcasses into palatability ('certified' or 'not certified' as palatable) groups. Carcasses (n = 769) were selected from four beef-packing plants to represent three USDA quality grade groups (Top Choice, TC; Low Choice, LC; and Select, SE). Following chilling, a prototype BeefCam image of the longissimus muscle was obtained for each carcass. Strip loins were collected from the left side of each carcass and aged for 10 d; Warner-Bratzler shear force (WBSF; n = 769) values and consumer panel ratings (hedonic, end-anchored, 9-point ratings for overall like/dislike; n = 500 carcasses) were obtained for cooked steaks. Using information from the images, two regression models were developed to predict the first principal component of WBSF and consumer panel ratings for sorting carcasses based on expected eating quality. Model I used only prototype BeefCam output, whereas Model II used prototype BeefCam output and a coded value for quality grade group. For both models, carcasses with a predicted value of less than 0.0 were certified as producing palatable beef. Additional carcasses (n = 292) were evaluated at a fifth and separate packing plant by prototype BeefCam to validate Models I and II. A strip loin was collected from each carcass and WBSF was measured after 14 d of aging. The percentages of validation carcasses that generated tough (WBSF [greater than or equal to] 4.5 kg) steaks were 6.5, 5.8, 10.7, and 7.9% for, TC, LC, SE, and all carcasses, respectively. Use of Model I certified 51.9, 47.6, 43.8, and 47.3% of TC, LC, SE, and all carcasses, respectively. Of the carcasses certified by use of Model I, 0.0, 0.0, 4.1, and 1.4% of TC, LC, SE, and all carcasses, respectively, generated tough steaks. Use of Model II certified 59.7, 47.6, 25.0, and 42.1% of TC, LC, SE, and all carcasses, respectively. Of the carcasses certified by use of Model II, 2.2, 0.0, 3.6, and 1.6% of TC, LC, SE, and all carcasses, respectively, generated tough steaks. For both models, the frequency of carcasses that produced tough steaks in the certified group was lower (P < 0.05) for all validation carcasses sampled compared with that of the original carcass population. Based on the decrease in the frequency of carcasses that produced tough steaks, further development of a commercial BeefCam system is warranted. Key Words: Beef, Classification, Palatability, Tenderness, Video Cameras

The National Beef Quality Audit-2000 was conducted to assess the current status of the quality and consistency of U.S. fed steers and heifers. Between May and November 2000, survey teams assessed hide condition (n = 43,415 cattle for color, brands, mud/manure), bruises (n = 43,595 carcasses), offal and carcass condemnation (n = 8,588 cattle), and carcass quality and yield information (n = 9,396 carcasses) in 30 U.S. beef packing plants. Hide colors were black (45.1%), red (31.0%), yellow (8.0%), Holstein (5.7%), gray (4.0%), white (3.2%), brown (1.7%), and brindle (1.3%). Brand frequencies were no (49.3%), one (46.2%), and two or more (4.4%), and brands were located on the butt (36.3%), side (13.7%), and shoulder (3.6%). Most cattle had no (18.0%) or a small amount (55.8%) of mud/manure on their hides, and they had no (77.3%) horns. Most carcasses (53.3%) were not bruised, 30.9% had one bruise, and 15.8% had multiple bruises. Bruise location and incidence were round (14.9%), loin (25.9%), rib (19.4%), chuck (28.2%), and brisket, flank, and plate (11.6%). Condemnation item and incidence were liver (30.3%), lungs (13.8%), tripe (11.6%), heads (6.2%), tongues (7.0%), and carcasses (0.1%). Carcass evaluation revealed these traits and frequencies: steer (67.9%), heifer (31.8%), and bullock (0.3%) sex-classes; dark-cutters (2.3%); A (96.6%), B (2.5%), and C or older (0.9%) overall maturities; and native (90.1%), dairy-type (6.9%), and Bos indicus (3.0%) breed-types. Mean USDA yield grade traits were USDA yield grade (3.0), carcass weight (356.9 kg), adjusted fat thickness (1.2 cm), longissimus muscle area (84.5 [cm.sup.2]), and kidney, pelvic, and heart fat (2.4%). USDA yield grades were Yield Grade 1 (12.2%), Yield Grade 2 (37.4%), Yield Grade 3 (38.6%), Yield Grade 4 (10.4%), and Yield Grade 5 (1.3%). Mean USDA quality grade traits were USDA quality grade ([Select.sup.85]), marbling score ([Small.sup.23]), overall maturity ([A.sup.66]), lean maturity ([A.sup.65]), and skeletal maturity ([A.sup.67]). Marbling score distribution was Slightly Abundant or higher (2.3%), Moderate (4.8%), Modest (13.1%), Small (33.3%), Slight (43.3%), and Traces (3.4%). USDA quality grades were Prime (2.0%), Choice (49.1%), Select (42.3%), Standard (5.6%), and Commercial, Utility, Cutter, and Canner (0.9%). This information will help the beef industry measure progress compared to the past two surveys and will provide a benchmark for future educational and research activities. Key Words: Beef Quality, Market Surveys, Meat Grades

Damaged beef muscle tissue resulting from intramuscular injections of animal-health products represents a 'quality control' problem and an economic loss to the beef industry. Fifteen individual and sequential national audits of injection-site lesions in beef top sirloin butts have been conducted at the steak provisioner/cutting level between November 1995 and July 2000. The national incidence of injection-site lesions in top sirloin butts (n = 240,080) decreased (P < 0.05) between November 1995 (11.4%) and July 2000 (2.1%). From November 1995 to July 1997, mean weight per injection-site lesion, across all lesion classes, increased (P < 0.05) from 192.5 g to 435.8 g, respectively; mean weight per lesion subsequently decreased (P < 0.05) to 249.8 g in July 2000 but was still heavier (P < 0.05) than in November 1995. Results of these audits suggest that producers have changed injection practices. These changes have likely been in response to educational efforts, such as those made by National Cattlemen's Beef Association and through state beef quality assurance programs. Analyses of results for lesion classes, partitioning lesions according to chronological stages of the healing process, suggested that the majority of lesions were induced at times that coincide with cow-calf, stocker, or early finishing-period stages of cattle production. Key Words: Beef, Damage, Injection, Lesions, Tissues

Presents information on a study which evaluated the effects of different applied beef carcass electrical stimulation treatments on muscle color, subprimal purge loss, cooked steak weight loss, and cooked steak tenderness. Materials and methodology; Results; Discussion on the study findings.