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Your search keyword '"Rodriguez, Raquel E."' showing total 35 results
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35 results on '"Rodriguez, Raquel E."'

18. Do fish feel pain?

Author

Rodriguez, Raquel E.

Subjects
Do Fish Feel Pain? (Nonfiction work) -- Braithwaite, Victoria -- Book reviews, Books -- Book reviews, Health care industry
Abstract

In the preface of her book, Victoria Braithwaite, with a humble vocabulary and an exquisite tone, accepts that she must look with open eyes at the recent controversy (for which [...]

Published
2010

21. Synthesis, biological and structural characterization of novel glycopeptide analogs of nociceptin

Author

Rodriguez, Raquel E., primary, Gonzalez‐Nuñez, Veronica, additional, Barreto‐Valer, Katerine, additional, Mayato, Carlos, additional, Dorta, Rosa L., additional, Trujillo‐Vazquez, Jesus, additional, Jimenez‐Barbero, Jesus, additional, Calle, Luis, additional, Marcelo, Filipa, additional, Morando, Maria, additional, Rosa, Monica, additional, Arsequell, Gemma, additional, and Valencia, Gregorio, additional

Published
2010
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27. In Vivo Regulation of NGF-Mediated Functions by Nedd4-2 Ubiquitination of TrkA.

Author

Tao Yu, Calvo, Laura, Anta, BegoŇa, López-Benito, Saray, López-Bellido, Roger, Vicente-García, Cristina, Tessarollo, Lino, Rodriguez, Raquel E., and Arévalo, Juan C.

Subjects
NERVE growth factor, NEUROTROPHIN receptors, UBIQUITINATION, GENETIC mutation, NEURONS
Abstract

Trk neurotrophin receptor ubiquitination in response to ligand activation regulates signaling, trafficking, and degradation of the receptors. However, the in vivo consequences of Trk ubiquitination remain to be addressed. We have developed a mouse model with a mutation in the TrkA neurotrophin receptor (P782S) that results in reduced ubiquitination due to a lack of binding to the E3 ubiquitin ligase, Nedd4-2. In vivo analyses of TrkAP782S indicate that defective ubiquitination of the TrkA mutant results in an altered trafficking and degradation of the receptor that affects the survival of sensory neurons. The dorsal root ganglia from the TrkAP782S knock-in mice display an increased number of neurons expressing CGRP and substance P. Moreover, the mutant mice show enhanced sensitivity to thermal and inflammatory pain. Our results indicate that the ubiquitination of the TrkA neurotrophin receptor plays a critical role in NGF-mediated functions, such as neuronal survival and sensitivity to pain. [ABSTRACT FROM AUTHOR]

Published
2014
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35. Characterization of a new duplicate delta-opioid receptor from zebrafish.

Author

Pinal-Seoane N, Martin IR, Gonzalez-Nuñez V, Marron Fernandez de Velasco E, Alvarez FA, Sarmiento RG, and Rodriguez RE

Subjects
Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cell Membrane metabolism, Cloning, Molecular, Conserved Sequence, DNA, Complementary genetics, Gene Expression, Humans, In Situ Hybridization, Molecular Sequence Data, Phylogeny, Protein Binding, Protein Isoforms chemistry, Protein Isoforms genetics, Protein Isoforms metabolism, RNA, Messenger genetics, Receptors, Opioid, delta genetics, Sequence Alignment, Zebrafish genetics, Receptors, Opioid, delta chemistry, Receptors, Opioid, delta metabolism, Zebrafish metabolism
Abstract

A new full-length cDNA (ZFOR4) that encodes an opioid receptor has been isolated from the teleost zebrafish. The encoded polypeptide is 375 amino acids long and shows high sequence similarity to other delta-opioid receptors, including ZFOR1, the other delta-opioid receptor from zebrafish previously characterized by us. In situ hybridization studies have revealed that ZFOR4 mRNA is highly expressed in particular brain areas that coincide with the expression of the delta-opioid receptor in other species. Pharmacological analysis of ZFOR4 shows specific and saturable binding with [(3)H] diprenorphine, displaying one binding site with K(D) = 3.42 +/- 0.38 nM and a receptor density of 6231 +/- 335 fmol/mg protein. Competition-binding experiments were performed using [(3)H]diprenorphine and several unlabelled ligands (peptidic and non-peptidic). The order of affinity obtained is Met-enkephalin>Naloxone>Leu-enkephalin>Dynorphin A>>BW373U86>Morphine>>>> [D-Pen(2),D-Pen(5)]-Enkephalin, U69,593. [(35)S]GTPgammaS stimulation studies show that the endogenous ligands Met- and Leu-enkephalin and the non-peptidic delta agonist BW373U86 were able to fully activate ZFOR4. Our results prove the existence of two functional duplicate genes of the delta-opioid receptor in the teleost zebrafish.

Published
2006
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