316 results on '"Rodrigues MT"'
Search Results
2. Levels of Ochratoxin in Urine and Possible Correlation with Food Consumption
- Author
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Rodrigues Mt, DuarteSC, Lino Cm, and Pena A
- Subjects
chemistry.chemical_compound ,Community pharmacy ,chemistry ,business.industry ,Endocrine pharmacology ,Food consumption ,Medicine ,Urine ,Food science ,Cardiovascular pharmacology ,Pharmacology ,business ,Ochratoxin - Published
- 2015
3. Moving beyond classical markers of water quality: detection of enteric viruses and genotoxicity in water of the Sinos River
- Author
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Bergamaschi,B, Rodrigues,MT, Silva,JVS, Kluge,M, Luz,RB, Fleck,JD., Bianchi,E, Silva,LB, and Spilki,FR
- Subjects
rotavirus ,genotoxicity ,adenovirus ,water quality ,enteroviruses - Published
- 2015
4. Adenovírus de origens canina e humana em fezes de graxains ( Lycalopex gymnocercus ) e cachorros-do-mato ( Cerdocyon thous ) de vida livre em São Francisco de Paula, bacia do Rio dos Sinos
- Author
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Monteiro, GS, Fleck, JD, Kluge, M, Rech, NK, Soliman, MC, Staggemeier, R, Rodrigues, MT, Barros, MP, Heinzelmann, LS, and Spilki, FR
- Subjects
rotavirus ,rotavírus ,enterovirus ,Lycalopex+gymnocercus<%2Fitalic>%22"> ,Lycalopex gymnocercus adenovírus ,enterovírus ,Cerdocyon+thous<%2Fitalic>%22"> Cerdocyon thous - Abstract
The spread of enteric viruses of domestic animals and human beings to wild species can be facilitated by the resistance of these viruses on the environment and their ability to be transmitted by water and contaminated food. The health status of the populations of pampas foxes (Lycalopex gymnocercus) and crab-eating foxes (Cerdocyon thous) is largely unknown and the landscapes occupied by these animals in southern Brazil have been threatened by human occupation and expansion of agriculture. In this work, the search of genomes of human and canine adenoviruses in feces from these wild carnivores was used to track the dissemination of domestic animals and human pathogens to the free-living populations in a wildlife reserve located in southern Brazil. This was performed by virus-specific differential real-time polymerase chain reactions (qPCR) on stool specimens, avoiding capture and additional stress to the animals. Genus-specific conventional reverse-transcriptase PCR (RT-PCR) was complementarily performed aiming the detection of enteroviruses (EV) and rotaviruses (RV) on these same samples. HAdV genomes were found on 14 out of the 17 (82.35%) stool samples analysed, whereas CAV was found co-infecting 5 of these samples. RV genomes were detected on 7 of the 17 samples (41.18%) and all samples were negative for EV. The results point to the dispersion of HAdV and RV at a high rate to these species of South American wild carnivores, which can be an effect of growing anthropisation of the habitat of these animals. A disseminação de vírus entéricos de animais domésticos e seres humanos para espécies selvagens pode ser facilitada pela resistência desses vírus no ambiente e sua capacidade de ser transmitida por água e alimentos contaminados. O estado de saúde das populações de Graxains-do-campo (Lycalopex gymnocercus) e Cachorros-do-mato (Cerdocyon thous) é em grande parte desconhecida e as paisagens ocupadas por estes animais no sul do Brasil têm sido ameaçadas pela ocupação humana e a expansão da agricultura. Neste trabalho, utilizou-se a pesquisa de genomas de adenovírus humanos (HAdV ) e caninos (CAV-1 e -2) em amostras fezes desses carnívoros selvagens com vistas a diagnosticar a disseminação de patógenos de animais domésticos e seres humanos às populações de vida livre em uma reserva de vida selvagem, localizado no sul do Brasil. Foram realizadas reações em cadeia da polimerase diferenciais e em tempo real (qPCR) de adenovírus específicos em amostras de fezes, evitando a captura e estresse adicional para os animais. PCRs gênero-específicas convencionais com transcrição reversa prévia (RT-PCR) foram ainda realizadas visando a detecção de enterovírus (EV) e rotavírus (RV) nestas mesmas amostras. Genomas de HAdV foram encontrados em 14 a 17 amostras de fezes (82.35%) analisados, Considerando que o CAV foi encontrado coinfectando 5 destas amostras. Genomas de RV foram detectados em 7 das 17 amostras (41.18%) e todas as amostras foram negativas para EV. Os resultados apontam para a dispersão de HAdV e RV em uma taxa elevada para estas espécies de carnívoros selvagens sul-americanas, que podem ser um efeito da crescente antropização do habitat desses animais.
- Published
- 2015
5. Monitoramento de metais, compostos orgânicos e coliforms em pontos de captação de água da bacia hidrográfica do Rio dos Sinos
- Author
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Nascimento,CA, Staggemeier,R, Bianchi,E, Rodrigues,MT, Fabres,R, Soliman,MC, Bortoluzzi,M, Luz,RB, Heinzelmann,LS, Santos,EL, Fleck,JD, and Spilki,FR
- Subjects
Rio dos Sinos ,uso do solo ,land usage ,qualidade da água ,water quality ,Sinos River - Abstract
Unplanned use and occupation of the land without respecting its capacity of assimilation and environmental purification leads to the degradation of the environment and of water used for human consumption. Agricultural areas, industrial plants and urban centres developed without planning and the control of effluent discharges are the main causes of water pollution in river basins that receive all the liquid effluents produced in those places. Over the last decades, environmental management has become part of governmental agendas in search of solutions for the preservation of water quality and the restoration of already degraded resources. This study evaluated the conditions of the main watercourse of the Sinos River basin by monitoring the main physical, chemical and microbiological parameters described in the CONAMA Resolution no. 357/2005.The set of parameters evaluated at five catchment points of water human consumption revealed a river that has different characteristics in each reach, as the upper reach was class 1, whereas the middle and lower reaches of the basin were class 4. Monitoring pointed to households as the main sources of pollutants in those reaches, although metals used in the industrial production of the region were found in the samples analyzed. O uso e ocupação do solo não planejados, sem respeitar sua capacidade de assimilação e de purificação ambiental, levam à degradação do ambiente e da água utilizada para consumo humano. Áreas agrícolas, plantas industriais e centros urbanos desenvolvidos sem planejamento e controle de descargas de efluentes são as principais causas de poluição da água em bacias hidrográficas que recebem todos os efluentes líquidos produzidos naqueles lugares. Nas últimas décadas, gestão ambiental tornou-se parte das agendas governamentais em busca de soluções para a preservação da qualidade da água e a restauração de recursos já degradadas. Este estudo avaliou as condições do curso de água principal da bacia do Rio dos Sinos, monitorando o principal físico, químico e microbiológicos parâmetros descritos na Resolução CONAMA n.º 357/2005. O conjunto de parâmetros avaliados em cinco pontos de captação de água para consumo humano revelou um rio que tem características diferentes em cada alcance, como o superior chegar era classe 1, Considerando que o atinge médio e inferior da bacia foram classe 4. monitoramento apontado famílias como as principais fontes de poluentes naqueles atinge, apesar de metais utilizados na produção industrial da região foram encontrados nas amostras analisadas.
- Published
- 2015
6. Adenoviruses of canine and human origins in stool samples from free-living pampas foxes (Lycalopex gymnocercus) and crab-eating foxes (Cerdocyon thous) in São Francisco de Paula, Rio dos Sinos basin
- Author
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Monteiro,GS, Fleck,JD, Kluge,M, Rech,NK, Soliman,MC, Staggemeier,R, Rodrigues,MT, Barros,MP, Heinzelmann,LS, and Spilki,FR
- Subjects
rotavirus ,enterovirus ,Lycalopex+gymnocercus<%2Fitalic>%22"> ,Lycalopex gymnocercus adenovírus ,Cerdocyon+thous<%2Fitalic>%22"> Cerdocyon thous - Published
- 2015
7. Moving beyond classical markers of water quality: detection of enteric viruses and genotoxicity in water of the Sinos River
- Author
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Bergamaschi, B, Rodrigues, MT, Silva, JVS, Kluge, M, Luz, RB, Fleck, JD., Bianchi, E, Silva, LB, and Spilki, FR
- Subjects
rotavirus ,rotavírus ,genotoxicity ,adenovirus ,adenovírus ,genotoxicidade ,enterovírus ,qualidade da água ,water quality ,enteroviruses - Abstract
It is well recognized that the classical biological and chemical markers of environmental pollution do not necessarily indicate the presence or absence of emerging threats to public health, such as waterborne viruses and genotoxicants. The purpose of this preliminary study was to evaluate the presence of material of enteroviruses (EV), rotavirus (RV) and adenovirus (AdV) and genotoxicity in water samples from points of routine monitoring of water quality in the main course of the Sinos River. The points are classified into different levels of pollution in accordance to the Brazilian federal regulations. Viral genomes from EV, AdV were detected in two of the 4 collection points regardless of the level of urbanisation of the surrounding areas. In contrast, genotoxicity was not observed in piava (Leporinus obtusidens) fingerlings cultivated on these same water samples. Results were compared with classical physical, chemical and microbiological parameters. There was no clear evidence of association between any of the classical markers and the presence of viral genomes in the water samples tested. É amplamente reconhecido que os marcadores biológicos e químicos clássicos para a poluição ambiental não necessariamente indicam a presença ou ausência de ameaças emergentes à saúde pública, tais como vírus transmitidos pela água e genotoxicantes. Este estudo preliminar teve por objetivo detectar material genético de enterovírus (EV), rotavírus (RV) e adenovírus (AdV) e genotoxicidade em amostras de água de pontos de monitoramento de rotina da qualidade da água no curso principal do rio dos Sinos. Os pontos são classificados em níveis diferentes de poluição, de acordo com as normativas federais brasileiras. Genomas virais de EV, RV e RV foram detectados em dois dos quatro pontos de coleta, independente do nível de urbanização das áreas adjacentes. Por outro lado, não foi observada genotoxicidade em alevinos de piava (Leporinus obtusidens) cultivados nestas mesmas amostras de água. Os resultados são comparados com marcadores físicos, químicos e microbiológicos clássicos, não há nenhuma evidência clara da associação entre qualquer um dos marcadores clássicos e da presença de genomas virais nas amostras de água testadas.
- Published
- 2015
8. Evaluation of Sinos River water genotoxicity using the comet assay in fish
- Author
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Scalon, MCS., Rechenmacher, C., Siebel, AM., Kayser, ML., Rodrigues, MT., Maluf, SW., Rodrigues, MAS., and Silva, LB
- Subjects
biomarcadores ,metais ,genotoxicity ,biomarkers ,metals ,river pollution ,genotoxicidade ,poluição de rios - Abstract
The Sinos River, in southern Brazil, is polluted by industrial discharges and untreated urban wastes. Fish genotoxicity biomarkers are valuable parameters for environmental risk assessment. In this study, we used the comet assay to detect genotoxicity due to multiple sources of pollution in the peripheral blood of a native fish species (Hyphessobrycon luetkenii). In addition, we analysed possible DNA damage from aluminum, lead, chromium, copper, nickel, iron and zinc contamination. Water samples were collected seasonally from three sampling sites and the fish were assessed under laboratory conditions. Water chemical analysis showed an increased level of aluminum and iron in most of the samples at sites 2 and 3, located in the middle and lower river course, respectively. The index of DNA damage assessed by the comet assay demonstrated no significant differences in different seasons or at the different sampling sites, while the frequency of cells with DNA damage was higher in water samples collected at sites 1 and 2 during the spring season. None of the metals studied seems to be associated with the increase in the frequency of cells with DNA damage observed during the spring season. The results of this study indicate that the Sinos River is contaminated with substances that are genotoxic to fish, including the waters near the river spring. O rio dos Sinos, no sul do Brasil, é poluído tanto por descargas industriais como por resíduos urbanos não tratados. Os biomarcadores de genotoxicidade em peixes são parâmetros valiosos para a determinação de risco ambiental. Neste estudo, utilizamos o ensaio cometa em sangue periférico de um peixe nativo (Hyphessobrycon luetkenii) para detectar a genotoxicidade devido a múltiplas fontes de poluição. Além disso, analisamos a possível influência da contaminação por alumínio, chumbo, cromo, cobre, níquel, ferro e zinco sobre o dano de DNA. Amostras de água foram coletadas sazonalmente em três pontos de coleta e os peixes foram expostos sob condições de laboratório. A análise química da água mostrou níveis elevados de alumínio e ferro na maioria das amostras dos pontos 2 e 3, localizados nos cursos médio e inferior do rio, respectivamente. O índice de dano de DNA estimado pelo ensaio cometa não demonstrou diferenças significativas tanto entre estações como entre pontos de coleta, enquanto que a frequência de células com dano de DNA apresentou valores superiores nas amostras de água coletadas durante a primavera nos pontos 1 e 2. Nenhum dos metais estudados parece estar associado com o aumento na frequência de células com dano de DNA observado durante a primavera. Os resultados deste estudo indicam que o rio dos Sinos está contaminado com substâncias genotóxicas para os peixes, incluindo as águas próximas à nascente do rio.
- Published
- 2010
9. Análise de múltiplos biomarcadores em ratos para determinar o impacto da poluição do Rio dos Sinos
- Author
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Rechenmacher,C., Siebel,AM., Goldoni,A., Klauck,CR., Sartori,T., Rodrigues,MT., Rodrigues,MAS., Gehlen,G., Ardenghi,PG., and Silva,LB
- Subjects
estresse oxidativo ,biomarcadores ,genotoxicity ,tissue damage ,dano tecidual ,pollution ,biomarkers ,oxidative stress ,genotoxicidade ,poluição - Abstract
The aim of this study was to determine the feasibility of combining water quality analysis with different biomarkers to characterise the relationship between anthropogenic contamination and biotic response in the Sinos River, southern Brazil. Wistar rats were studied using three biomarkers combined with physical, chemical and microbiological analysis to assess the effects of pollution at four sampling sites. The induction of oxidative stress was quantified by MDA levels in peripheral blood, lymphocyte DNA damage was determined using the comet assay, and histopathological changes were analysed in the liver. After sampling, animals were allowed to drink the river water during a 48 hours period. No increase in oxidative stress and DNA damage was observed. However, liver damage was observed in the animals exposed to water samples, indicating that the Sinos River is contaminated with hepatotoxic substances. Water analyses confirmed that water quality decreased downriver. O objetivo deste estudo foi determinar a viabilidade de combinar a análise da qualidade da água com o estudo de biomarcadores para caracterizar a relação entre contaminação antropogênica e resposta biótica no rio dos Sinos, sul do Brasil. Ratos Wistar machos foram estudados usando três biomarcadores combinados com análises físicas, químicas e microbiológicas para determinar os efeitos da poluição em quatro locais de amostragem de água. A indução do estresse oxidativo foi quantificada pelos níveis de MDA no sangue periférico, o dano do DNA linfocitário foi determinado usando o ensaio cometa, e as alterações histopatológicas foram analisadas no fígado. As amostras da água do rio dos Sinos (dos quatro pontos selecionados) foram administradas por via oral (como água para beber) por 48 horas. Nenhum aumento do estresse oxidativo ou do dano do DNA foi observado. Entretanto, dano do tecido hepático foi verificado nos animais expostos à ingestão das amostras da água, indicando que o rio dos Sinos está contaminado com substâncias hepatotóxicas. As análises das amostras de água confirmaram a redução da qualidade da água em direção à foz do rio.
- Published
- 2010
10. Moving beyond classical markers of water quality: detection of enteric viruses and genotoxicity in water of the Sinos River
- Author
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Bergamaschi, B, primary, Rodrigues, MT, additional, Silva, JVS, additional, Kluge, M, additional, Luz, RB, additional, Fleck, JD., additional, Bianchi, E, additional, Silva, LB, additional, and Spilki, FR, additional
- Published
- 2015
- Full Text
- View/download PDF
11. Adenoviruses of canine and human origins in stool samples from free-living pampas foxes (Lycalopex gymnocercus) and crab-eating foxes (Cerdocyon thous) in São Francisco de Paula, Rio dos Sinos basin
- Author
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Monteiro, GS, primary, Fleck, JD, additional, Kluge, M, additional, Rech, NK, additional, Soliman, MC, additional, Staggemeier, R, additional, Rodrigues, MT, additional, Barros, MP, additional, Heinzelmann, LS, additional, and Spilki, FR, additional
- Published
- 2015
- Full Text
- View/download PDF
12. Monitoring of metals, organic compounds and coliforms in water catchment points from the Sinos River basin
- Author
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Nascimento, CA, primary, Staggemeier, R, additional, Bianchi, E, additional, Rodrigues, MT, additional, Fabres, R, additional, Soliman, MC, additional, Bortoluzzi, M, additional, Luz, RB, additional, Heinzelmann, LS, additional, Santos, EL, additional, Fleck, JD, additional, and Spilki, FR, additional
- Published
- 2015
- Full Text
- View/download PDF
13. Molecular techniques for the study and diagnosis of parasite infection
- Author
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Tavares, RG, primary, Staggemeier, R, additional, Borges, ALP, additional, Rodrigues, MT, additional, Castelan, LA, additional, Vasconcelos, J, additional, Anschau, ME, additional, and Spalding, SM, additional
- Published
- 2011
- Full Text
- View/download PDF
14. A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil
- Author
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Rechenmacher, C., primary, Siebel, AM., additional, Goldoni, A., additional, Klauck, CR., additional, Sartori, T., additional, Rodrigues, MT., additional, Rodrigues, MAS., additional, Gehlen, G., additional, Ardenghi, PG., additional, and Silva, LB, additional
- Published
- 2010
- Full Text
- View/download PDF
15. Evaluation of Sinos River water genotoxicity using the comet assay in fish
- Author
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Scalon, MCS., primary, Rechenmacher, C., additional, Siebel, AM., additional, Kayser, ML., additional, Rodrigues, MT., additional, Maluf, SW., additional, Rodrigues, MAS., additional, and Silva, LB, additional
- Published
- 2010
- Full Text
- View/download PDF
16. Profile of Eicosanoids in Breath Condensate in Asthma and COPD.
- Author
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Fritscher, LG, primary, Rodrigues, MT, additional, Sporn, H, additional, Balter, M, additional, Post, M, additional, Zamel, N, additional, and Chapman, KR, additional
- Published
- 2009
- Full Text
- View/download PDF
17. Unilateral patent nasopalatine duct: a case report and review of the literature.
- Author
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Rodrigues MT, Munhoz EA, Cardoso CL, Junior OF, and Damante JH
- Published
- 2009
- Full Text
- View/download PDF
18. Immunomodulatory Behavior of Tendon Magnetic Cell Sheets can be Modulated in Hypoxic Environments under Magnetic Stimulus.
- Author
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Vinhas A, Rodrigues MT, Gonçalves AI, and Gomes ME
- Subjects
- Humans, Cell Hypoxia, Electromagnetic Fields, Interleukin-1beta metabolism, Cells, Cultured, Magnetite Nanoparticles chemistry, NF-kappa B metabolism, Tendons cytology, Tendons radiation effects
- Abstract
Tissue environments play a crucial role in orchestrating cell behavior, guided by a complex interplay of various factors. Long lasting inflammatory signals compromise tendon homeostasis and promote tissue degeneration, while tissue oxygen levels affect local cells' responses with hypoxic environments influencing apoptosis, inflammatory mediators, and matrix production. Recent works have unveiled the therapeutic potential of pulsed electromagnetic field (PEMF) in modulating inflammatory signals expressed by human tendon cells (hTDCs), and in mitigating the hypoxia-induced effects on the regulation of inflammatory cytokines. Thus, we sought to investigate the role of hypoxic environments, namely, 1 and 2% oxygen tension, in the inflammatory profiles of magnetic cell sheets (magCSs) formed by magnetic nanoparticles internalized in contiguous hTDCs with intact cell-cell junctions and deposited matrix. We also aimed to explore the impact of PEMF over hypoxia-treated magCSs, including IL-1β-primed-magCSs, with the objective of harnessing magnetic stimulation to guide abnormal inflammatory cell responses toward efficient treatments supporting tendon regenerative potential. Our findings revealed that low oxygen tensions amplified the expression of hypoxia-associated genes and of inflammatory markers in IL-1β-primed-magCSs with an involvement of the NF-κB signaling pathway. Encouragingly, when PEMF was applied to IL-1β-primed-magCSs under hypoxic conditions, it successfully modulated inflammatory cues by favoring IL-10 and IL-4, via the NF-κB pathway. These results signify the remarkable potential of PEMF in driving proregenerative strategies and opens up new approaches in tendon therapies, highlighting the transformative impact of immunomodulatory magnetic cell sheets.
- Published
- 2024
- Full Text
- View/download PDF
19. Liver Biopsy Technique for Analysis of Hepatic Content during Pregnancy and Early Lactation in Dairy Goats.
- Author
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de Oliveira AM, de Faria ALS, Quirino DF, Schultz ÉB, Rennó LN, Rodrigues MT, and Veloso CM
- Abstract
Biopsy techniques in dairy goats are currently limited. This study aimed to describe a liver biopsy technique in dairy goats and to evaluate liver triglyceride levels and glycogen content. Sixty-nine dairy goats in the final stage of pregnancy and early lactation period were selected. Fifty goats were selected randomly for hepatic biopsy (HB) according to gestational period and were characterized according to fetus number (single: n = 16, multiple: n = 34), supplementation with propylene glycol (diet: n = 23, diet+PG: n = 27), and milk production levels (high: 3.0 ± 0.4 L/day, n = 15; low: 1.4 ± 0.4 L/day, n = 26). Liver tissue samples were obtained through biopsy on days -30, -20, -15, -10, -5, and 15 days after calving. Hepatic triglyceride and glycogen were quantified. The results were analyzed using the F-test at a 5% significance level and a comparison of means using the Tukey test. The liver biopsies did not influence dry matter intake, body weight, or milk yield. Hepatic glycogen concentration was lower 15 days after calving than it was prior to calving, except on day -20. Goats that generated high levels of milk production had lower triglyceride levels than goats that generated low levels of milk production. The biopsy technique is a safe method for obtaining tissue and evaluating liver content in dairy goats. The milk production level and days relative to parturition influence the hepatic triglyceride and glycogen content in dairy goats.
- Published
- 2024
- Full Text
- View/download PDF
20. A Dynamic Tool to Describe Lamb Growth and Its Use as a Decision Support System.
- Author
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Amaral RM, Rodrigues MT, Schultz EB, and Reis CER
- Abstract
A dynamic model has been developed to simulate aspects of feedlot lamb growth and body composition, including energy and protein requirements, growth rate, composition of gain, and body mass. Model inputs include initial body mass (kg), standard final mass (kg), age (days), and dietary energy concentration (Mcal·kg
-1 ). The model was assessed as a decision support tool using a dataset of 564 individual measures of final body mass and diet energy. The simulations provide graphical and numerical descriptions of nutrient requirements, composition of gain, and estimates of animal performance over time. The model is accurate and precise, with a root mean squared error of 7.79% of the observed final body mass and a coefficient of determination of 0.89 when simulating the same variable. The model can be used as a reliable decision support tool to estimate final body mass and the days on feed required to reach a certain final mass with precision and accuracy. Moreover, the dynamic model can also serve as a learning tool to illustrate practical principles of animal nutrition, nutrient requirement relationships, and body composition changes. This model holds the potential to enhance livestock management practices and assist producers in making informed decisions about feedlot lamb production.- Published
- 2024
- Full Text
- View/download PDF
21. Screening of the Skin-Regenerative Potential of Antimicrobial Peptides: Clavanin A, Clavanin-MO, and Mastoparan-MO.
- Author
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Alencar-Silva T, Díaz-Martín RD, Sousa Dos Santos M, Saraiva RVP, Leite ML, de Oliveira Rodrigues MT, Pogue R, Andrade R, Falconi Costa F, Brito N, Dias SC, and Carvalho JL
- Subjects
- Humans, Intercellular Signaling Peptides and Proteins metabolism, Antimicrobial Peptides pharmacology, Cells, Cultured, Peptides pharmacology, Wound Healing drug effects, Skin metabolism, Skin drug effects, Cell Proliferation drug effects, Cell Movement drug effects, Fibroblasts drug effects, Fibroblasts metabolism, Regeneration drug effects
- Abstract
Skin wound healing is coordinated by a delicate balance between proinflammatory and anti-inflammatory responses, which can be affected by opportunistic pathogens and metabolic or vascular diseases. Several antimicrobial peptides (AMPs) possess immunomodulatory properties, suggesting their potential to support skin wound healing. Here, we evaluated the proregenerative activity of three recently described AMPs (Clavanin A, Clavanin-MO, and Mastoparan-MO). Human primary dermal fibroblasts (hFibs) were used to determine peptide toxicity and their capacity to induce cell proliferation and migration. Furthermore, mRNA analysis was used to investigate the modulation of genes associated with skin regeneration. Subsequently, the regenerative potential of the peptides was further confirmed using an ex vivo organotypic model of human skin (hOSEC)-based lesion. Our results indicate that the three molecules evaluated in this study have regenerative potential at nontoxic doses (i.e., 200 μM for Clavanin-A and Clavanin-MO, and 6.25 μM for Mastoparan-MO). At these concentrations, all peptides promoted the proliferation and migration of hFibs during in vitro assays. Such processes were accompanied by gene expression signatures related to skin regenerative processes, including significantly higher KI67 , HAS2 and CXCR4 mRNA levels induced by Clavanin A and Mastoparan-MO. Such findings translated into significantly accelerated wound healing promoted by both Clavanin A and Mastoparan-MO in hOSEC-based lesions. Overall, the data demonstrate the proregenerative properties of these peptides using human experimental skin models, with Mastoparan-MO and Clavanin A showing much greater potential for inducing wound healing compared to Clavanin-MO.
- Published
- 2024
- Full Text
- View/download PDF
22. Editorial: Advanced strategies to bridge the gap between inflammation and tissue regeneration.
- Author
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Rodrigues MT and Gomes ME
- Subjects
- Humans, Animals, Inflammation, Regeneration physiology
- Published
- 2024
- Full Text
- View/download PDF
23. Bismuth(III) triflate: an economical and environmentally friendly catalyst for the Nazarov reaction.
- Author
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Rodrigues MT Jr, de Oliveira ASB, Gomes RC, Hirata AS, Zeoly LA, Santos H, Arantes J, Reis-Silva CSM, Machado-Neto JA, Costa-Lotufo LV, and Coelho F
- Abstract
We describe the use of bismuth(III) triflate as an efficient and environmentally friendly catalyst for the Nazarov reaction of aryl vinyl ketones, leading to the synthesis of 3-aryl-2-ethoxycarbonyl-1-indanones and 3-aryl-1-indanones. By changing the temperature and reaction time, it was possible to modulate the reactivity, allowing the synthesis of two distinct product classes (3-aryl-2-ethoxycarbonyl-1-indanones and 3-aryl-1-indanones) in good to excellent yield. The reaction did not require additives and was insensitive to both air and moisture. Preliminary biological evaluation of some indanones showed a promising profile against some human cancer line cells., (Copyright © 2024, Rodrigues Jr. et al.)
- Published
- 2024
- Full Text
- View/download PDF
24. Correction: Understanding the Role of Growth Factors in Modulating Stem Cell Tenogenesis.
- Author
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Gonçalves AI, Rodrigues MT, Lee SJ, Atala A, Yoo JJ, Reis RL, and Gomes ME
- Abstract
[This corrects the article DOI: 10.1371/journal.pone.0083734.]., (Copyright: © 2024 Gonçalves et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Published
- 2024
- Full Text
- View/download PDF
25. Does heat tolerance actually predict animals' geographic thermal limits?
- Author
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Camacho A, Rodrigues MT, Jayyusi R, Harun M, Geraci M, Carretero MA, Vinagre C, and Tejedo M
- Subjects
- Animals, Acclimatization, Climate Change, Temperature, Amphibians, Fishes, Mammals, Thermotolerance, Arthropods
- Abstract
The "climate extremes hypothesis" is a major assumption of geographic studies of heat tolerance and climatic vulnerability. However, this assumption remains vastly untested across taxa, and multiple factors may contribute to uncoupling heat tolerance estimates and geographic limits. Our dataset includes 1000 entries of heat tolerance data and maximum temperatures for each species' known geographic limits (hereafter, Tmax). We gathered this information across major animal taxa, including marine fish, terrestrial arthropods, amphibians, non-avian reptiles, birds, and mammals. We first tested if heat tolerance constrains the Tmax of sites where species could be observed. Secondly, we tested if the strength of such restrictions depends on how high Tmax is relative to heat tolerance. Thirdly, we correlated the different estimates of Tmax among them and across species. Restrictions are strong for amphibians, arthropods, and birds but often weak or inconsistent for reptiles and mammals. Marine fish describe a non-linear relationship that contrasts with terrestrial groups. Traditional heat tolerance measures in thermal vulnerability studies, like panting temperatures and the upper set point of preferred temperatures, do not predict Tmax or are inversely correlated to it, respectively. Heat tolerance restricts the geographic warm edges more strongly for species that reach sites with higher Tmax for their heat tolerance. These emerging patterns underline the importance of reliable species' heat tolerance indexes to identify their thermal vulnerability at their warm range edges. Besides, the tight correlations of Tmax estimates across on-land microhabitats support a view of multiple types of thermal challenges simultaneously shaping ranges' warm edges for on-land species. The heterogeneous correlation of Tmax estimates in the ocean supports the view that fish thermoregulation is generally limited, too. We propose new hypotheses to understand thermal restrictions on animal distribution., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Catarina Vinagre reports financial support was provided by Foundation for Science and Technology. Miguel Trefaut Rodrigues reports financial support was provided by State of Sao Paulo Research Foundation. Miguel Tejedo reports financial support was provided by Secretaría de Estado de Investigación, Desarrollo e Innovación. Agustín Camacho Guerrero reports financial support was provided by Council of the European Union., (Copyright © 2024. Published by Elsevier B.V.)
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- 2024
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26. Diversification of the terrestrial frog genus Anomaloglossus (Anura, Aromobatidae) in the Guiana Shield proceeded from highlands to lowlands, with successive loss and reacquisition of endotrophy.
- Author
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Vacher JP, Kok PJR, Rodrigues MT, Lima A, Hrbek T, Werneck FP, Manzi S, Thébaud C, and Fouquet A
- Subjects
- Animals, Phylogeny, Phylogeography, Anura genetics, Ecosystem
- Abstract
Two main landscapes emerge from the Guiana Shield: the highlands to the west called the Pantepui region and the Amazonian lowlands to the east, both harbouring numerous endemic species. With 32 currently recognized species, the genus Anomaloglossus stands out among Neotropical frogs as one that diversified only within the Guiana Shield both in the highlands and the lowlands. We present a time-calibrated phylogeny obtained by using combined mitogenomic and nuclear DNA, which suggests that the genus originates from Pantepui where extant lineages started diversifying around 21 Ma, and subsequently (ca. 17 Ma) dispersed during the Miocene Climatic Optimum to the lowlands of the eastern Guiana Shield where the ability to produce endotrophic tadpoles evolved. Further diversification within the lowlands in the A. stepheni group notably led to an evolutionary reversal toward exotrophy in one species group during the late Miocene, followed by reacquisition of endotrophy during the Pleistocene. These successive shifts of reproductive mode seem to have accompanied climatic oscillations. Long dry periods might have triggered evolution of exotrophy, whereas wetter climates favoured endotrophic forms, enabling colonization of terrestrial habitats distant from water. Acquisition, loss, and reacquisition of endotrophy makes Anomaloglossus unique among frogs and may largely explain the current species diversity. The micro evolutionary processes involved in these rapid shifts of reproductive mode remain to be revealed., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)
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- 2024
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27. YY1 mutations disrupt corticogenesis through a cell-type specific rewiring of cell-autonomous and non-cell-autonomous transcriptional programs.
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Pereira MF, Finazzi V, Rizzuti L, Aprile D, Aiello V, Mollica L, Riva M, Soriani C, Dossena F, Shyti R, Castaldi D, Tenderini E, Carminho-Rodrigues MT, Bally JF, de Vries BBA, Gabriele M, Vitriolo A, and Testa G
- Abstract
Germline mutations of YY1 cause Gabriele-de Vries syndrome (GADEVS), a neurodevelopmental disorder featuring intellectual disability and a wide range of systemic manifestations. To dissect the cellular and molecular mechanisms underlying GADEVS, we combined large-scale imaging, single-cell multiomics and gene regulatory network reconstruction in 2D and 3D patient-derived physiopathologically relevant cell lineages. YY1 haploinsufficiency causes a pervasive alteration of cell type specific transcriptional networks, disrupting corticogenesis at the level of neural progenitors and terminally differentiated neurons, including cytoarchitectural defects reminiscent of GADEVS clinical features. Transcriptional alterations in neurons propagated to neighboring astrocytes through a major non-cell autonomous pro-inflammatory effect that grounds the rationale for modulatory interventions. Together, neurodevelopmental trajectories, synaptic formation and neuronal-astrocyte cross talk emerged as salient domains of YY1 dosage-dependent vulnerability. Mechanistically, cell-type resolved reconstruction of gene regulatory networks uncovered the regulatory interplay between YY1, NEUROG2 and ETV5 and its aberrant rewiring in GADEVS. Our findings underscore the reach of advanced in vitro models in capturing developmental antecedents of clinical features and exposing their underlying mechanisms to guide the search for targeted interventions., Competing Interests: Competing Interest: All authors declare no competing interests.
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- 2024
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28. Relicts in the mist: Two new frog families, genera and species highlight the role of Pantepui as a biodiversity museum throughout the Cenozoic.
- Author
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Fouquet A, Kok PJR, Recoder RS, Prates I, Camacho A, Marques-Souza S, Ghellere JM, McDiarmid RW, and Rodrigues MT
- Subjects
- Animals, Phylogeny, Biodiversity, Biological Evolution, Museums, Anura genetics
- Abstract
The iconic mountains of the Pantepui biogeographical region host many early-diverging endemic animal and plant lineages, concurring with Conan Doyle's novel about an ancient "Lost World". While this is the case of several frog lineages, others appear to have more recent origins, adding to the controversy around the diversification processes in this region. Due to its remoteness, Pantepui is challenging for biological surveys, and only a glimpse of its biodiversity has been described, which hampers comprehensive evolutionary studies in many groups. During a recent expedition to the Neblina massif on the Brazil-Venezuela border, we sampled two new frog species that could not be assigned to any known genus. Here, we perform phylogenetic analyses of mitogenomic and nuclear loci to infer the evolutionary relationships of the new taxa and support their description. We find that both species represent single lineages deeply nested within Brachycephaloidea, a major Neotropical clade of direct-developing frogs. Both species diverged >45 Ma from their closest relatives: the first is sister to all other Brachycephaloidea except for Ceuthomantis, another Pantepui endemic, and the second is sister to Brachycephalidae, endemic to the Brazilian Atlantic Forest. In addition to these considerable phylogenetic and biogeographic divergences, external morphology and osteological features support the proposition of two new family and genus-level taxa to accommodate these new branches of the amphibian tree of life. These findings add to other recently described ancient vertebrate lineages from the Neblina massif, providing a bewildering reminder that our perception of the Pantepui's biodiversity remains vastly incomplete. It also provides insights into how these mountains acted as "museums" during the diversification of Brachycephaloidea and of Neotropical biotas more broadly, in line with the influential "Plateau theory". Finally, these discoveries point at the yet unknown branches of the tree of life that may go extinct, due to global climate change and zoonotic diseases, before we even learn about their existence, amphibians living at higher elevations being particularly at risk., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)
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- 2024
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29. Effects of chemical aging on carbonaceous materials: Stability of water-dispersible colloids and their influence on the aggregation of natural-soil colloid.
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Fregolente LG, Rodrigues MT, Oliveira NC, Araújo BS, Nascimento ÍV, Souza Filho AG, Paula AJ, Costa MCG, Mota JCA, and Ferreira OP
- Abstract
Although hydrochar and biochar have been used as soil conditioners, there is not a clear understanding of how their properties changes due to aging impacts their colloidal particles behavior on the soil system. From this premise, we produced hydrochar and biochar from the same feedstock (cashew bagasse) and aged with different chemical methods: (i) using hydrogen peroxide, (ii) a mixture of nitric and sulfuric acids, and (iii) hot water. It was analyzed the effects of aging on the stability of the carbonaceous materials (CMs) colloids in aqueous medium with different ionic strength (single systems), as well as the stability of the natural-soil colloid when interacting with biochar and hydrochar colloids (binary systems). A chemical composition (C, H, N, and O content) change in CMs due to the chemically induced aging was observed along with minor structural modifications. Chemical aging could increase the amount of oxygen functional groups for both biochar and hydrochar, though in a different level depending on the methodology applied. In this sense, hydrochar was more susceptive to chemical oxidation than biochar. The effectiveness of chemical aging treatments for biochar increased in the order of water < acid < hydrogen peroxide, whereas for hydrochar the order was water < hydrogen peroxide < acid. While the increase in surface oxidation improved the biochar colloidal stability in water medium at different ionic strengths (single systems), the stability and critical coagulation concentration (CCC) slightly changed for hydrochar. Natural-soil clay (NSC) interactions with oxidized carbonaceous material colloids (binary systems) enhanced NSC stability, which is less likely to aggregate. Therefore, the aging of carbonaceous materials modifies the interaction and dynamics of soil small particles, requiring far more attention to the environmental risks due to their application over time., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
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30. Lysicamine Reduces Protein Kinase B (AKT) Activation and Promotes Necrosis in Anaplastic Thyroid Cancer.
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Rodrigues MT, Michelli APP, Caso GF, de Oliveira PR, Rodrigues-Junior DM, Morale MG, Machado Júnior J, Bortoluci KR, Tamura RE, da Silva TRC, Raminelli C, Chau E, Godin B, Calil-Silveira J, and Rubio IGS
- Abstract
Anaplastic thyroid cancer (ATC) is an aggressive form of thyroid cancer (TC), accounting for 50% of total TC-related deaths. Although therapeutic approaches against TC have improved in recent years, the survival rate remains low, and severe adverse effects are commonly reported. However, unexplored alternatives based on natural compounds, such as lysicamine, an alkaloid found in plants with established cytotoxicity against breast and liver cancers, offer promise. Therefore, this study aimed to explore the antineoplastic effects of lysicamine in papillary TC (BCPAP) and ATC (HTH83 and KTC-2) cells. Lysicamine treatment reduced cell viability, motility, colony formation, and AKT activation while increasing the percentage of necrotic cells. The absence of caspase activity confirmed apoptosis-independent cell death. Necrostatin-1 (NEC-1)-mediated necrosome inhibition reduced lysicamine-induced necrosis in KTC-2, suggesting necroptosis induction via a reactive oxygen species (ROS)-independent mechanism. Additionally, in silico analysis predicted lysicamine target proteins, particularly those related to MAPK and TGF-β signaling. Our study demonstrated lysicamine's potential as an antineoplastic compound in ATC cells with a proposed mechanism related to inhibiting AKT activation and inducing cell death.
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- 2023
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31. Effects of forage quality and particle size on feed intake and ruminoreticulum content of goats.
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Lopes DS, Rodrigues MT, and de Oliveira TS
- Abstract
The aim was to evaluate the effect of particle size and hay quality on feed intake, granulometric profile, and composition of the ruminoreticulum content in goats. We used 54 Alpine bucks in a completely randomized design with a factorial arrangement of 3 × 3. Treatments were a combination of Bermuda grass hay ( Cynodon dactylon ) with three quality levels: high (35 days), medium (50 days), and low (65 d) harvested at regrowth times. Were evaluated three particle sizes: small (16% ≥4.76 mm), medium (48% ≥4.76 mm), and large (75% ≥4.76 mm), which accounted for 66%, 75%, and 94% of physically effective fiber, respectively. Samples of offered diet, intake, and ruminoreticulum content were used to generate the granulometric profile. The offered diet, intake, and ruminoreticulum content presented different granulometric profiles regarding hay quality and particle size. Dry matter intake (DMI) and neutral detergent fiber intake (NDFI) increased ( P < 0.05) when low-quality hay and large particles were offered. However, when particle size in low-quality hay was reduced, DMI and NDF decreased ( P < 0.05). When analyzing the ruminoreticulum content (DM, NDF, peNDF, and indigestible DM), we did not observe any effect ( P > 0.05) of hay quality or particle size on the variables. Thus, reducing hay quality and increasing particle size increase dry matter and fiber intake, presenting an interaction between forage quality and particle size. Forage quality and particle size promote intense selective behavior and chewing, which leads to a homogeneous content of particle profile in ruminoreticulum and a uniform average retention time., Competing Interests: The authors declare no conflicts of interest., (© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science.)
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- 2023
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32. Dehydration alters behavioral thermoregulation and the geography of climatic vulnerability in two Amazonian lizards.
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Camacho A, Brunes TO, and Rodrigues MT
- Subjects
- Animals, Dehydration, Body Temperature Regulation physiology, Body Temperature physiology, Temperature, Geography, Lizards physiology
- Abstract
High temperatures and low water availability often strike organisms concomitantly. Observing how organisms behaviorally thermohydroregulate may help us to better understand their climatic vulnerability. This is especially important for tropical forest lizards, species that are purportedly under greater climatic risk. Here, we observed the influence of hydration level on the Voluntary Thermal Maximum (VTmax) in two small Amazonian lizard species: Loxopholis ferreirai (semiaquatic and scansorial) and Loxopholis percarinatum (leaf litter parthenogenetic dweller), accounting for several potential confounding factors (handling, body mass, starting temperature and heating rate). Next, we used two modeling approaches (simple mapping of thermal margins and NicheMapR) to compare the effects of dehydration, decrease in precipitation, ability to burrow, and tree cover availability, on geographic models of climatic vulnerability. We found that VTmax decreased with dehydration, starting temperature, and heating rates in both species. The two modeling approaches showed that dehydration may alter the expected intensity, extent, and duration of perceived thermal risk across the Amazon basin for these forest lizards. Based on our results and previous studies, we identify new evidence needed to better understand thermohydroregulation and to model the geography of climatic risk using the VTmax., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Camacho et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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- 2023
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33. Critical Contribution of Imbalanced Charge Loss to Performance Deterioration of Si-Based Lithium-Ion Cells during Calendar Aging.
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Cai J, Zhou X, Li T, Nguyen HT, Veith GM, Qin Y, Lu W, Trask SE, Fonseca Rodrigues MT, Liu Y, Xu W, Schulze MC, Burrell AK, and Chen Z
- Abstract
Increasing the energy density of lithium-ion batteries, and thereby reducing costs, is a major target for industry and academic research. One of the best opportunities is to replace the traditional graphite anode with a high-capacity anode material, such as silicon. However, Si-based lithium-ion batteries have been widely reported to suffer from a limited calendar life for automobile applications. Heretofore, there lacks a fundamental understanding of calendar aging for rationally developing mitigation strategies. Both open-circuit voltage and voltage-hold aging protocols were utilized to characterize the aging behavior of Si-based cells. Particularly, a high-precision leakage current measurement was applied to quantitatively measure the rate of parasitic reactions at the electrode/electrolyte interface. The rate of parasitic reactions at the Si anode was found 5 times and 15 times faster than those of LiNi
0.8 Mn0.1 Co0.1 O2 and LiFePO4 cathodes, respectively. The imbalanced charge loss from parasitic reactions plays a critical role in exacerbating performance deterioration. In addition, a linear relationship between capacity loss and charge consumption from parasitic reactions provides fundamental support to assess calendar life through voltage-hold tests. These new findings imply that longer calendar life can be achieved by suppressing parasitic reactions at the Si anode to balance charge consumption during calendar aging.- Published
- 2023
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34. Biocompatible 3D-Printed Tendon/Ligament Scaffolds Based on Polylactic Acid/Graphite Nanoplatelet Composites.
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Silva M, Gomes S, Correia C, Peixoto D, Vinhas A, Rodrigues MT, Gomes ME, Covas JA, Paiva MC, and Alves NM
- Abstract
Three-dimensional (3D) printing technology has become a popular tool to produce complex structures. It has great potential in the regenerative medicine field to produce customizable and reproducible scaffolds with high control of dimensions and porosity. This study was focused on the investigation of new biocompatible and biodegradable 3D-printed scaffolds with suitable mechanical properties to assist tendon and ligament regeneration. Polylactic acid (PLA) scaffolds were reinforced with 0.5 wt.% of functionalized graphite nanoplatelets decorated with silver nanoparticles ((f-EG)+Ag). The functionalization of graphene was carried out to strengthen the interface with the polymer. (f-EG)+Ag exhibited antibacterial properties against Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ), an important feature for the healing process and prevention of bacterial infections. The scaffolds' structure, biodegradation, and mechanical properties were assessed to confirm their suitability for tendon and ligamentregeneration. All scaffolds exhibited surface nanoroughness created during printing, which was increased by the filler presence. The wet state dynamic mechanical analysis proved that the incorporation of reinforcement led to an increase in the storage modulus, compared with neat PLA. The cytotoxicity assays using L929 fibroblasts showed that the scaffolds were biocompatible. The PLA+[(f-EG)+Ag] scaffolds were also loaded with human tendon-derived cells and showed their capability to maintain the tenogenic commitment with an increase in the gene expression of specific tendon/ligament-related markers. The results demonstrate the potential application of these new 3D-printed nanocomposite scaffolds for tendon and ligament regeneration.
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- 2023
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35. Contactless Resolution of Inflammatory Signals in Tailored Macrophage-Based Cell Therapeutics.
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Almeida AF, Miranda MS, Vinhas A, Rodrigues MT, and Gomes ME
- Abstract
In recent years, nanotechnology-based microRNA (miR) therapeutic platforms have shown great promise for immunotherapy and tissue regeneration, despite the unmet challenge of achieving efficient and safe delivery of miRs. The transport of miRs offers precision and regulatory value for a myriad of biological processes and pathways, including the control of macrophage (Mφ) functions and, consequently, the inflammatory cascades Mφ are involved in. Thus, enforcement of Mφ can boost the regenerative process and provide new solutions for diverse chronic pathologies. In this study, we sought to develop a magnetically guided transporter to deliver an miR-155 antagonist to M1-primed Mφ. Furthermore, we determined its modulatory effect in reprogramming Mφ from inflammatory to pro-regenerative phenotypes, with the aim of tissue healing and regenerative medicine approaches. This strategy combines contactless and high-precision control of Mφ, anticipating new functional miR carriers for targeted strategies controlled by extracorporeal action. The magnetoplexes SPION@PEI-miR were efficiently delivered into Mφ without compromising cell viability and successfully induced miR-mediated gene silencing by enhancing the expression of anti-inflammatory markers (IL4 and IL10) and the production of M2φ-related markers (CD206 and IL4). Given its multimodal features, SPION@PEI-miR represents a simple, safe, and nonviral theranostic platform that enables imaging, tracking, and miR delivery with modulatory effects on immune cells.
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- 2023
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36. One-pot organocatalyzed synthesis of tricyclic indolizines.
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Zeoly LA, Acconcia LV, Rodrigues MT Jr, Santos H, Cormanich RA, Paniagua JC, Moyano A, and Coelho F
- Abstract
Indolizines and their saturated derivatives are important structural motifs present in several biologically active compounds of both natural and synthetic origin. We describe herein a one-pot approach for the synthesis of tricyclic indolizines catalyzed by a bicyclic imidazole-alcohol. The protocol is based on an aqueous Morita-Baylis-Hillman reaction between pyridine-2-carboxaldehydes and six- or seven-membered cyclic enones, followed by sequential intramolecular cyclization and dehydration. So, in a single operational step two new bonds (C-C and C-N) are formed in an organocatalyzed process that takes place in simple conditions (stirring in water at 60 °C for 12 h) and with great atom economy (water as the sole byproduct), affording the purified compounds in yields ranging from 19 to 70%. The facility of the cyclization strongly depends on the size of the cycloalkenone ring: while MBH adducts derived from six-, seven- or eight-membered cycloenones are readily transformed into the corresponding indolizines, cyclopentenone-derived MBH adducts do not cyclize. A competition experiment revealed that cycloheptenone-derived MBH adducts cyclize faster than cyclohexenone-derived adducts. Model DFT calculations have been performed to rationalize these reactivity trends.
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- 2023
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37. Prospects of magnetically based approaches addressing inflammation in tendon tissues.
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Vinhas A, Almeida AF, Rodrigues MT, and Gomes ME
- Subjects
- Humans, Electromagnetic Fields, Magnetic Fields, Inflammation therapy, Tendons, Wound Healing
- Abstract
Tendon afflictions constitute a significant share of musculoskeletal diseases and represent a primary cause of incapacity worldwide. Unresolved/chronic inflammatory states have been associated with the onset and progression of tendon disorders, contributing to undesirable immune stimulation and detrimental tissue effects. Thus, targeting persistent inflammatory events could assist important developments to solve pathophysiological processes and innovative therapeutics to address impaired healing and accomplish complete tendon regeneration. This review overviews the impact of inflammation and inflammatory mediators in tendon niches, unveiling the importance of tendon cell populations and their signature features, and the influence of microenvironmental factors on inflamed and injured tendons. The demand for non-invasive instructive strategies to manage persistent inflammatory mediators, guide inflammatory pathways, and modulate cellular responses will also be approached by exploring the role of pulsed electromagnetic field (PEMF). PEMF alone or combined with more sophisticated systems triggered by magnetic fields will be considered in the design of successful therapies to control inflammation in tendinopathic conditions., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)
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- 2023
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38. The endotrophic nidicolous tadpole of Cycloramphus eleutherodactylus<br>(Miranda-Ribeiro) (Anura: Cycloramphidae).
- Author
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Verdade VK, Almeida-Silva D, and Rodrigues MT
- Subjects
- Animals, Larva, Forests, Anura, Ranidae
- Abstract
Cycloramphus Tschudi is the most speciose genus in the family Cycloramphidae, including 30 species endemic to the Atlantic forest Domain in Brazil (Frost 2022). Species in the genus may be classified as saxicolous and terrestrial based on morphology and reproductive mode. Saxicolous are flat-bodied species, with granular skin, and interdigital web (Heyer 1983a), associated with fast-flowing streams in all life history phases. They lay eggs over humid rocks, logs, or crevices in the splash zone, from which semiterrestrial tadpoles hatch (McDiarmid & Altig 1999; Dias et al. 2021) that feed over humid rocks inside the stream or dripping rocky walls (Lutz 1929; Heyer 1983a, b; Haddad & Sazima 1989; Giaretta & Cardoso 1995; Giaretta & Facure 2003; Lima et al. 2010). The single exception to this pattern is C. bandeirensis Heyer 1983a, which share the morphology of adults and larvae, but lives terrestrially, away from streams, in the rocky fields of open high elevation grasslands of Caparaó National Park (Verdade et al. 2019).
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- 2023
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39. Testing assertions of widespread introgressive hybridization in a clade of neotropical toads with low mate selectivity (Rhinella granulosa species group).
- Author
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Rivera D, Prates I, Caldwell JP, Rodrigues MT, and Fujita MK
- Subjects
- Gene Flow, Genome, Hybridization, Genetic, Phylogeny, Sexual Behavior, Behavior, Animal, DNA, Mitochondrial genetics, Genetic Introgression, Anura genetics
- Abstract
Discordance between different genomic regions, often identified through multilocus sequencing of selected markers, presents particular difficulties in identifying historical processes which drive species diversity and boundaries. Mechanisms causing discordance, such as incomplete lineage sorting or introgression due to interspecific hybridization, are better identified based on population-level genomic datasets. In the toads of the Rhinella granulosa species group, patterns of mito-nuclear discordance and potential hybridization have been reported by several studies. However, these patterns were proposed based on few loci, such that alternative mechanisms behind gene-tree heterogeneity cannot be ruled out. Using genome-wide ddRADseq loci from a subset of species within this clade, we found only partial concordance between currently recognized species-level taxon boundaries and patterns of genetic structure. While most taxa within the R. granulosa group correspond to clades, genetic clustering analyses sometimes grouped distinct taxonomic units into a single cluster. Moreover, levels of admixture between inferred clusters were limited and restricted to a single taxon pair which is best explained by incomplete lineage sorting as opposed to introgressive hybridization, according to D-statistics results. These findings contradict previous assertions of widespread cryptic diversity and gene flow within the R. granulosa clade. Lastly, our analyses suggest that diversification events within the Rhinella granulosa group mostly dated back to the early Pliocene, being generally younger than species divergences in other closely related clades that present high levels of cross-species gene flow. This finding uniquely contradicts common assertions that this young clade of toads exhibits interspecific hybridization., (© 2022. The Author(s), under exclusive licence to The Genetics Society.)
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- 2023
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40. Controlling Macrophage Polarization to Modulate Inflammatory Cues Using Immune-Switch Nanoparticles.
- Author
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Almeida AF, Miranda MS, Vinhas A, Gonçalves AI, Gomes ME, and Rodrigues MT
- Subjects
- Humans, Macrophages metabolism, Inflammation Mediators metabolism, Inflammation metabolism, Macrophage Activation, Nanoparticles
- Abstract
The persistence of inflammatory mediators in tissue niches significantly impacts regenerative outcomes and contributes to chronic diseases. Interleukin-4 (IL4) boosts pro-healing phenotypes in macrophages (Mφ) and triggers the activation of signal transducer and activator of transcription 6 (STAT6). Since the IL4/STAT6 pathway reduces Mφ responsiveness to inflammation in a targeted and precise manner, IL4 delivery offers personalized possibilities to overcome inflammatory events. Despite its therapeutic potential, the limited success of IL4-targeted delivery is hampered by inefficient vehicles. Magnetically assisted technologies offer precise and tunable nanodevices for the delivery of cytokines by combining contactless modulation, high tissue penetration, imaging features, and low interference with the biological environment. Although superparamagnetic iron oxide nanoparticles (SPION) have shown clinical applicability in imaging, SPION-based approaches have rarely been explored for targeted delivery and cell programming. Herein, we hypothesized that SPION-based carriers assist in efficient IL4 delivery to Mφ, favoring a pro-regenerative phenotype (M2φ). Our results confirmed the efficiency of SPION-IL4 and Mφ responsiveness to SPION-IL4 with evidence of STAT6-mediated polarization. SPION-IL4-treated Mφ showed increased expression of M2φ associated-mediators (IL10, ARG1, CCL2, IL1Ra) when compared to the well-established soluble IL4. The ability of SPION-IL4 to direct Mφ polarization using sophisticated magnetic nanotools is valuable for resolving inflammation and assisting innovative strategies for chronic inflammatory conditions.
- Published
- 2022
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41. A new Chiasmocleis (Anura: Microhylidae) from the eastern Guiana Shield with an amended definition of C. haddadi Peloso, Sturaro, Forlani, Gaucher, Motta, & Wheeler, 2014.
- Author
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Fouquet A, Rodrigues MT, and Peloso P
- Subjects
- Animals, Guyana, Anura genetics, Anura anatomy & histology, Plant Breeding
- Abstract
The existence of an unnamed (candidate) species from French Guiana and the state of Amapá (Brazil) closely related to Chiasmocleis haddadi has been hypothesized in previous DNA-based studies. With an integrative use of genetic, morphological and acoustic data we confirm that these populations are indeed distinct from C. haddadi and all other known congeners. We herein describe and name this new species, and amend the definition of C. haddadi since the type-series and its original diagnosis included specimens of the new species. Geographically, the new species occurs eastward of its sister species C. haddadi from which it can be distinguished by having a larger body, a proportionally smaller eye and a distinct dorsal colouration. We suspect that the new species breeds in the leaf litter and that tadpoles undergo endotrophic development whereas co-occurring C. haddadi and C. hudsoni lay clutches in the leaf litter nearby standing water of temporary ponds where exotrophic tadpoles complete their development. This new species is a new addition to the already long list of animals being endemic to the easternmost part of the Guiana Shield, a region identified as a singular bioregion within Amazonia by previous research.
- Published
- 2022
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42. New Occurrences of the Tiger Shark ( Galeocerdo cuvier) (Carcharhinidae) off the Coast of Rio de Janeiro, Southeastern Brazil: Seasonality Indications.
- Author
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Aximoff I, Cumplido R, Rodrigues MT, de Melo UG, Fagundes Netto EB, Santos SR, and Hauser-Davis RA
- Abstract
The tiger shark Galeocerdo cuvier (Péron & Lesueur, 1822) (Carcharhinidae) is classified as near-threatened along the Brazilian coast, in line with its global categorization. Although Rio de Janeiro, located in southeastern Brazil, is internationally identified as a priority shark conservation area, many shark species, including tiger sharks, are landed by both industrial and artisanal fisheries in this state. However, there is a lack of detailed information on the species capture pressures and records for the state of Rio de Janeiro. Therefore, the aims of this study were to expand the tiger shark record database and to improve upon future conservation and management strategies. Tiger shark records from four coastal Rio de Janeiro regions were obtained by direct observation. The information obtained from fishery colonies/associations, environmental guards, researchers, and scientific articles, totaling 23 records, resulted in an approximately 5-fold increase in the number of tiger shark records off the coast of the state of Rio de Janeiro. A possible seasonality pattern concerning the size of the captured/observed animals was noted, emphasizing the need to consider the coast of Rio de Janeiro as an especially relevant area for at least part of the life history of tiger sharks.
- Published
- 2022
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43. An HNRNPK-specific DNA methylation signature makes sense of missense variants and expands the phenotypic spectrum of Au-Kline syndrome.
- Author
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Choufani S, McNiven V, Cytrynbaum C, Jangjoo M, Adam MP, Bjornsson HT, Harris J, Dyment DA, Graham GE, Nezarati MM, Aul RB, Castiglioni C, Breckpot J, Devriendt K, Stewart H, Banos-Pinero B, Mehta S, Sandford R, Dunn C, Mathevet R, van Maldergem L, Piard J, Brischoux-Boucher E, Vitobello A, Faivre L, Bournez M, Tran-Mau F, Maystadt I, Fernández-Jaén A, Alvarez S, García-Prieto ID, Alkuraya FS, Alsaif HS, Rahbeeni Z, El-Akouri K, Al-Mureikhi M, Spillmann RC, Shashi V, Sanchez-Lara PA, Graham JM Jr, Roberts A, Chorin O, Evrony GD, Kraatari-Tiri M, Dudding-Byth T, Richardson A, Hunt D, Hamilton L, Dyack S, Mendelsohn BA, Rodríguez N, Sánchez-Martínez R, Tenorio-Castaño J, Nevado J, Lapunzina P, Tirado P, Carminho Amaro Rodrigues MT, Quteineh L, Innes AM, Kline AD, Au PYB, and Weksberg R
- Subjects
- Abnormalities, Multiple, Chromatin, Epigenesis, Genetic, Face abnormalities, Hematologic Diseases, Heterogeneous-Nuclear Ribonucleoprotein K genetics, Humans, Phenotype, Vestibular Diseases, DNA Methylation genetics, Intellectual Disability genetics
- Abstract
Au-Kline syndrome (AKS) is a neurodevelopmental disorder associated with multiple malformations and a characteristic facial gestalt. The first individuals ascertained carried de novo loss-of-function (LoF) variants in HNRNPK. Here, we report 32 individuals with AKS (26 previously unpublished), including 13 with de novo missense variants. We propose new clinical diagnostic criteria for AKS that differentiate it from the clinically overlapping Kabuki syndrome and describe a significant phenotypic expansion to include individuals with missense variants who present with subtle facial features and few or no malformations. Many gene-specific DNA methylation (DNAm) signatures have been identified for neurodevelopmental syndromes. Because HNRNPK has roles in chromatin and epigenetic regulation, we hypothesized that pathogenic variants in HNRNPK may be associated with a specific DNAm signature. Here, we report a unique DNAm signature for AKS due to LoF HNRNPK variants, distinct from controls and Kabuki syndrome. This DNAm signature is also identified in some individuals with de novo HNRNPK missense variants, confirming their pathogenicity and the phenotypic expansion of AKS to include more subtle phenotypes. Furthermore, we report that some individuals with missense variants have an "intermediate" DNAm signature that parallels their milder clinical presentation, suggesting the presence of an epi-genotype phenotype correlation. In summary, the AKS DNAm signature may help elucidate the underlying pathophysiology of AKS. This DNAm signature also effectively supported clinical syndrome delineation and is a valuable aid for variant interpretation in individuals where a clinical diagnosis of AKS is unclear, particularly for mild presentations., Competing Interests: Declaration of interests H.T.B. is a consultant for Mahzi therapeutics., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2022
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44. Induced resistance to ifosfamide in osteosarcoma cells suggests a more aggressive tumor profile.
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de Oliveira Rodrigues MT, Pereira da Silva L, Pogue RE, de Carvalho JL, Motoyama AB, de Alencar E Silva T, Brunel HDSS, de Sá MFG, and Vieira de Andrade R
- Abstract
Aims: Osteosarcoma (OS) is the most common primary malignant bone sarcoma among children and adolescents. Treatment is based on neo-adjuvant and adjuvant chemotherapy, using the standard drugs cisplatin, methotrexate, doxorubicin, and ifosfamide (IFO). Due to the high capacity of tumor resistance, the current work aimed to analyze genes related to cycle control and cell differentiation in OS cells sensitive to and with induced resistance to IFO. This was to assess whether the differentiated expression of these genes may affect resistance to the drug IFO used in OS treatment, and thus establish possible biomarkers of disease progression., Materials and Methods: In this work, the treatment-sensitive OS U2OS lineage was used, and the same lineage was submitted to the process of induction of IFO resistance. These cells were evaluated by MTT, migration and proliferation assays and submitted to gene expression analysis., Key Findings: The results demonstrate that after induction of resistance to IFO, resistant U2OS cells show a more aggressive tumor behavior, with greater capacity for cell migration, proliferation, and invasion compared to sensitive cells. Gene analysis indicates that resistance-induced cells have differentiated expression of the genes EPB41L3 , GADD45A , IER3, OXCT1, UBE2L6 , UBE2A ALPL , and EFNB2. Our results suggest new perspectives on possible resistance biomarkers, especially the genes EFNB2 and EPB41L3 , given that these genes have rarely been studied their expression linked to osteosarcoma. They show how the resistance induction model can be useful for studies on tumor cell behavior ., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2022 Published by Elsevier B.V.)
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- 2022
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45. Magnetic Micellar Nanovehicles: Prospects of Multifunctional Hybrid Systems for Precision Theranostics.
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Miranda MS, Almeida AF, Gomes ME, and Rodrigues MT
- Subjects
- Contrast Media, Drug Delivery Systems methods, Polymers chemistry, Precision Medicine, Hyperthermia, Induced, Micelles
- Abstract
Hybrid nanoarchitectures such as magnetic polymeric micelles (MPMs) are among the most promising nanotechnology-enabled materials for biomedical applications combining the benefits of polymeric micelles and magnetic nanoparticles within a single bioinstructive system. MPMs are formed by the self-assembly of polymer amphiphiles above the critical micelle concentration, generating a colloidal structure with a hydrophobic core and a hydrophilic shell incorporating magnetic particles (MNPs) in one of the segments. MPMs have been investigated most prominently as contrast agents for magnetic resonance imaging (MRI), as heat generators in hyperthermia treatments, and as magnetic-susceptible nanocarriers for the delivery and release of therapeutic agents. The versatility of MPMs constitutes a powerful route to ultrasensitive, precise, and multifunctional diagnostic and therapeutic vehicles for the treatment of a wide range of pathologies. Although MPMs have been significantly explored for MRI and cancer therapy, MPMs are multipurpose functional units, widening their applicability into less expected fields of research such as bioengineering and regenerative medicine. Herein, we aim to review published reports of the last five years about MPMs concerning their structure and fabrication methods as well as their current and foreseen expectations for advanced biomedical applications.
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- 2022
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46. Controlling the fate of regenerative cells with engineered platelet-derived extracellular vesicles.
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Graça AL, Gómez-Florit M, Osório H, Rodrigues MT, Domingues RMA, Reis RL, and Gomes ME
- Subjects
- Culture Media, Endothelial Cells, Proteomics, Reproducibility of Results, Extracellular Vesicles metabolism, Mesenchymal Stem Cells metabolism
- Abstract
Extracellular vesicles (EVs) have emerged as cell-free nanotherapeutic agents for the potential treatment of multiple diseases and for tissue engineering and regenerative medicine strategies. Nevertheless, the field has typically relied on EVs derived from stem cells, the production of which in high quantities and high reproducibility is still under debate. Platelet-derived EVs were produced by a freeze-thaw method of platelet concentrates, a highly available clinical waste material. The aim of this study was to produce and thoroughly characterize platelet-derived EVs and understand their effects in adipose-tissue derived stem cells (hASCs), endothelial cells (HUVECs) and macrophages. Two different EV populations were obtained after differential centrifugation, namely small EVs (sEVs) and medium EVs (mEVs), which showed different size distributions and unique proteomic signatures. EV interaction with hASCs resulted in the modulation of the gene expression of markers related to their commitment toward different lineages. Moreover, mEVs showed higher angiogenic potential than sEVs, in a tube formation assay with HUVECs. Also, the EVs were able to modulate macrophage polarization. Altogether, these results suggest that platelet-derived EVs are promising candidates to be used as biochemical signals or therapeutic tools in tissue engineering and regenerative medicine approaches.
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- 2022
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47. Diversity, biogeography, and reproductive evolution in the genus Pipa (Amphibia: Anura: Pipidae).
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Fouquet A, Cornuault J, Rodrigues MT, Werneck FP, Hrbek T, Acosta-Galvis AR, Massemin D, Kok PJR, and Ernst R
- Subjects
- Amphibians genetics, Animals, Bayes Theorem, DNA, Mitochondrial genetics, Phylogeny, Phylogeography, Pipidae genetics
- Abstract
The genus Pipa is a species-poor clade of Neotropical frogs and one of the most bizarre-looking due to many highly derived anatomical traits related to their fully aquatic lifestyle. With their African relatives, they form the Pipidae family, which has attracted much attention, especially regarding its anatomy, reproductive biology, paleontology and biogeography. However, the actual diversity and phylogenetic relationships within Pipa remain poorly understood, and thus so do their historical biogeography and the evolution of striking features, such as the absence of teeth and endotrophy in some species. Using short mtDNA sequences across the distribution of the genus, we identified 15 main lineages (Operational Taxonomic Units - OTUs). This more than doubles the number of the currently seven valid nominal species. Several closely related OTUs do not share nuDNA alleles, confirming species divergence. Time-calibrated phylogenies obtained from mitogenomes and from 10 nuclear loci provide highly similar topologies but strikingly distinct node ages for Pipa. High dN/dS ratios and the variation of substitution rates across the trees suggest a strong effect of saturation on fast evolving positions of mtDNA, producing a substantially shorter stem branch of Pipa. Focusing on the nuDNA topology, we inferred an early Neogene Amazonian origin of the diversification of Pipa, with an initial split between the Guiana-Brazilian Shields and Western Amazonia, a pattern observed in many other co-distributed groups. All the western species are edentate, suggesting a single loss in the genus. Each of these groups diversified further out of Amazonia, toward the Atlantic Forest and toward trans-Andean forests, respectively. These events are concomitant with paleogeographic changes and match patterns observed in other co-distributed taxonomic groups. The two Amazonian lineages have probably independently acquired endotrophic larval development., (Copyright © 2022 Elsevier Inc. All rights reserved.)
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- 2022
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48. Corrigendum to "The ontogenetic allometry of body morphology and chemical composition in dairy goat wethers" [Animal 12(3) (2018) 538-553].
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Vieira RAM, Rohem Júnior NM, Gomes RS, Oliveira TS, Bendia LCR, Azevedo FHV, Barbosa DL, Glória LS, and Rodrigues MT
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- 2022
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49. Morphological variation and genealogical discordance in Caatinga sand lizards Calyptommatus Rodrigues 1991 (Squamata: Gymnophthalmidae) with the description of a new species.
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Recoder RS, Marques-Souza S, Silva-Soares T, Ramiro CN, Castro TM, and Rodrigues MT
- Subjects
- Animals, DNA, Mitochondrial genetics, Ecosystem, Lizards
- Abstract
In the last decades, a remarkable fauna of psammophilous and fossorial squamates was discovered in sandy habitats of the semiarid Caatinga of northeast Brazil. Despite the increasing accumulation of genetic data from this unique fauna, an incomplete knowledge of its diversity still hampers a better understanding of its origins and diversification. The fossorial lizard genus Calyptommatus (Gymnophthalmidae) is endemic to sandy habitats of the Caatinga, being currently represented by four allopatric species. In this study, we used morphological and molecular data to assess population-level variation in Calyptommatus. We found a new morphotype of Calyptommatus from the state of Bahia, Brazil, readily distinguished from congeners by the presence of a frontal scale. Morphological, nuDNA and geographic data support the recognition of a new species herein described as Calyptommatus frontalis sp. nov. Nevertheless, genetic data revealed mito-nuclear+morphology discordance, with populations with frontal scales distributed in three distantly related mtDNA clades, suggesting either potential historical and/or current introgressions or incomplete lineage sorting. Further data are needed to clarify the status of the two other mtDNA clades displaying a frontal scale.
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- 2022
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50. Two new species of geckos of the genus Phyllopezus Peters, 1878 (Squamata: Gekkota: Phyllodactylidae) from northeastern Brazil.
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Dubeux MJM, Gonalves U, Palmeira CNS, Nunes PMS, Cassimiro J, Gamble T, Werneck FP, Rodrigues MT, and Mott T
- Subjects
- Animals, Brazil, Ecosystem, Forests, Genetic Drift, Lizards
- Abstract
We describe two new species of Brazilian geckos of the genus Phyllopezus based on morphological and molecular data. The first species is currently known from a relictual Cerrado enclavecampos rupestres, in the mountains of the Serra do Espinhao in the state of Bahia. The second species is known from northeastern Atlantic Forest and transitional areas with Caatinga biome in the state of Alagoas. The two new species are sister taxa and together are the sister clade to the remaining species in the Phyllopezus pollicaris species complex. These new species can be morphologically distinguished from their congeners by meristic and morphometric characters, in addition to color pattern and genetic differentiation.
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- 2022
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