1. Comparative study of the neural differentiation capacity of mesenchymal stromal cells from different tissue sources: An approach for their use in neural regeneration therapies
- Author
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Claudio M. Jofre, Rodrigo Ruiz Mardones, Daniela N. Urrutia, Pablo Caviedes, Ana María Vega-Letter, and José J. Minguell
- Subjects
0301 basic medicine ,Male ,Embryology ,medicine.medical_treatment ,Cellular differentiation ,Cell- and Tissue-Based Therapy ,Adipose tissue ,Gene Expression ,Regenerative Medicine ,Biochemistry ,Umbilical Cord ,Nestin ,0302 clinical medicine ,Animal Cells ,Central Nervous System Diseases ,Neurobiology of Disease and Regeneration ,Medicine and Health Sciences ,Chile ,Cells, Cultured ,Skin ,Neurons ,Multidisciplinary ,Stem Cells ,Neurogenesis ,Cell Differentiation ,Stem-cell therapy ,Cell biology ,medicine.anatomical_structure ,Neurology ,Adipose Tissue ,Medicine ,Female ,Cellular Types ,Neuronal Differentiation ,Research Article ,Adult ,Science ,Primary Cell Culture ,Bone Marrow Cells ,Biology ,Neuroprotection ,03 medical and health sciences ,Young Adult ,medicine ,Genetics ,Humans ,Progenitor cell ,Cell Proliferation ,Mesenchymal stem cell ,Biology and Life Sciences ,Proteins ,Mesenchymal Stem Cells ,Cell Biology ,Nerve Regeneration ,Cytoskeletal Proteins ,030104 developmental biology ,Cellular Neuroscience ,Bone marrow ,030217 neurology & neurosurgery ,Developmental Biology ,Neuroscience - Abstract
Mesenchymal stem cells (MSCs) can trans/differentiate to neural precursors and/or mature neurons and promote neuroprotection and neurogenesis. The above could greatly benefit neurodegenerative disorders as well as in the treatment of post-traumatic and hereditary diseases of the central nervous system (CNS). In order to attain an ideal source of adult MSCs for the treatment of CNS diseases, adipose tissue, bone marrow, skin and umbilical cord derived MSCs were isolated and studied to explore differences with regard to neural differentiation capacity. In this study, we demonstrated that MSCs from several tissues can differentiate into neuron-like cells and differentially express progenitors and mature neural markers. Adipose tissue MSCs exhibited significantly higher expression of neural markers and had a faster proliferation rate. Our results suggest that adipose tissue MSCs are the best candidates for the use in neurological diseases.
- Published
- 2019