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5 results on '"Rodrigo Otávio de Faria"'

1. The Tyrosinase Produced by Lentinula boryana (Berk. & Mont.) Pegler Suffers Substrate Inhibition by L-DOPA

Author

Rodrigo Otávio de Faria, Vivian Rotuno Moure, Wellington Balmant, Maria Angela Lopes de Almeida Amazonas, Nadia Krieger, and David Alexander Mitchell

Subjects
tyrosinase, Lentinula boryana, substrate inhibition, 3,4-dihydroxy-L-phenylalanine, L-DOPA, Biotechnology, TP248.13-248.65, Food processing and manufacture, TP368-456
Abstract

We undertook a preliminary characterization of the tyrosinase produced by a strain of Lentinula boryana from Brazil, with a view to evaluate its potential for biotechnological applications. The enzyme was similar to other fungal tyrosinases in many respects. When the crude extract was characterized, the tyrosinase activity was optimal at pH=6 and was not particularly thermostable, with half-lives of about 10 min and 1 min at 50 and 60 °C, respectively. We purified the enzyme with ammonium sulfate precipitation followed by ion exchange chromatography on a DEAE Sepharose column, obtaining a yield of 33 % and a 5.3-fold enrichment. The purified preparation gave three bands on SDS-PAGE, with molecular masses of 20, 27 and 47 kDa. This preparation showed substrate inhibition kinetics with L-DOPA (3,4-dihydroxy-L-phenylalanine), with a KM of 1.9 mM and a KI of 72 mM. Under the same reaction conditions, a commercial mushroom tyrosinase followed Michaelis-Menten kinetics, with a KM of 0.51 mM. Although the present study did not identify properties that would make the tyrosinase of L. boryana more suitable in biotechnological applications than tyrosinases from other mushrooms, it has made a contribution by showing that the enzyme suffers substrate inhibition by L-DOPA, something that has not previously been reported for mushroom tyrosinases.

Published
2007

2. The Biotechnological Potential of Mushroom Tyrosinases

Author

Rodrigo Otávio de Faria, Vivian Rotuno Moure, Maria Angela Lopes de Almeida Amazonas, David Alexander Mitchell, and Nadia Krieger

Subjects
mushroom tyrosinase, Agaricus bisporus, biosensors, cross-linked biopolymers, phenol removal, submerged culture, Biotechnology, TP248.13-248.65, Food processing and manufacture, TP368-456
Abstract

Over the last decade there has been a significant interest in developing biotechnological applications of tyrosinases. These applications include the production of L-DOPA (3,4-dihydroxyphenyl-L-alanine) from L-tyrosine, the production of cross-linked protein networks for use as novel food additives and the detection of phenolic compounds in wastewater or their removal from it. Much of the research into these applications has involved mushroom tyrosinases. We review the potential biotechnological applications of mushroom tyrosinases and evaluate the state of knowledge about their production, recovery and immobilization. We conclude that much more research is necessary in these areas if mushroom tyrosinases are to fulfill their biotechnological potential.

Published
2007

3. Epidemiologia e Políticas Públicas de Saúde

Author

Lucas Kenji Takeishi, Vladmir Machado Rios, Atílio Franco De Mattos, Benedito Silva De Almeida Junior, Luiz Muller Pimentel Delfim Lacerda, Diego José Pereira Lopes, Rômulo Micael Lacerda Vieira, João Vitor Dias Ramos, Emilio Guedes Cocate, Fernanda Lima Marçal, Lara Rhania Canedo Carvalho, Fernanda Odete Souza Rodrigues, Luciano Scher Fernandes, Kaique Augusto Marques de Campos, Luis Felipe Silveira Mega, Gabriela Silveira Mega, Luiza Amarante Rodrigues, Giovanna Domingues Ribeiro, Brenda Nayara Gontijo de Moura, Giovanna Neves Cartagena Miranda, Juliana Costa Ribeiro, Catalina Gómez Scarpelli, Mariana Abdo de Almeida, Letícia Christiane Huida dos Santos, Karina de Castro Barbosa, Ágata Raposo de Medeiros, Camila Alves de Bessa, Diulya Fontenele França, Thayssa Souza Sandes, DANYELLY RODRIGUES MACHADO AZEVEDO, Ana Clara Oliveira Domingues, Ana Carolina Xavier Souto, Camila Martins Soares, Gerliane Ribeiro Vilela, Isabella Stéfanny de Freitas Postigo, Murillo Waldo Silva Fleury, Diego Barbosa Resende, Amanda Gabriele Alves Cobiniano De Melo, Amanda Guimarães Loureiro, Ana Carolina Pompeu Rosa, Eunice De Oliveira Costa, Guilherme Aires Oliveira Campos, Luann Bambach Marinho, Maria Luíza Alves Cobiniano De Melo, Marta Regina Silva Oliveira, Mayara De Andrade Moratto, João Felipe Tinto Silva, Felipe Santana e Silva, Andréa Gomes De Almeida, Cloves De Moraes Carvalho Neto, Deijane Colaço Pinto, Erick Romulo Bezerra De Sousa, Guilherme Andrade Carneiro, Iracema Silva Da Costa, Jaqueline Brito Da Costa, Laine Da Costa Almeida Barboza, Washington Vittor Da Conceição Silva, Híllary Ruth Rodrigues Da Silva, Antonia Mylene Sousa Almeida, Shirley De Vasconcelos Bonfim Brito, Luiz Gerson Gonçalves Neto, Laís Mesquita de Sousa, João Victor Salvador, Giovana Perez de Souza, Mariana de Oliveira Costa, Giuliana Lugarini, Ana Flávia Moreira, Paola Lugarini, Thiago Barbosa Fernandes, Lara Macedo Lembrance, Maria Carolina Sticanele de Souza, Mariana Rodrigues De Queiroz, Nelmara Alvarenga Vieira, Viviane Nogueira de Paiva, Davi Soares Pinto, Phelipe Von Der Heide Sarmento, Fernanda Gabry Scazuza Gomes de Souza, Davi Silva Santana, Vanessa Ladyanne Da Silva Costa, Letícia Gomes de Oliveira, Anna Beatriz De Souza Piedade, Yngrid Monteiro Da Silva, Williany Álvares Da Silva Barros, Pedro Paulo Santos Nunes, Yasmin Marçal Soares Miranda, Giovana Rodrigues Puga, Leidiane De Jesus Da Costa Santos, Maria Eduarda De Araújo Moraes, Jayany Shirlley Pereira Da Silva, Valéria Gabriele Caldas Nascimento, Camila Dos Santos Moraes, Ingrid Inez Dos Santos Amaral, Manoel Cícero Viana de Lima, Ysabele Yngrydh Valente Silva, Maria Clara de Freitas Albano, Madeline Medeiros Oliveira, Francisco Vittor Miranda e Araújo, Ana Beatriz Sousa Santos, Jesamar correia Matos filho, Sofia Pereira Lopes, Samuel Carvalho Vasconcelos, Gabriel Romão Mesquita do Nascimento, Ruddy Mariano Maia Cysne Guerra, João Ferreira de Paula Neto, Pedro Schmitt Martins Paiva Matos, Amanda Almeida Lima, Kilvia Maria Albuquerque, Caroline Dambrós, Caroline Possamai Rovadoscki, Cristian Gabriel Hermes, Ianne Layla Santos Nunes, Renata Marim da Silveira, João M P F Nunes, Daniel Rubens, Wallace Girão da Silva, Monique Marques de Souza, Catarina de J Nunes, Iriel Ferreira Passos, Laís Ramos de Oliveira, Davi Salles, Zeila Cristina Pereira Pimenta, Milena Silva Guimarães, Silvia Evangelista Santos, Jéssica Lara Cipriano Santos, Mariana Tenório Costa, Giovanna Mendonça dos Santos, Ataianny Karine Siqueira Almeida, Pedro Emanuel Ramos de Lima, Weberton Dorásio Sobrinho, Yasmim Vieira dos Santos, Thaina Safira Souza da Costa, Woodyson Welson Barros da Silva Batista, Dayana Moraes Lopes, Victória Gonçalves Neves, Maria Helena de Lima Sousa, Maria Alice Araújo de França Carvalho, Airton César Leite, Ana Victória da Silva Santos, Lucas Eduardo Galvão Alves Moraes, Geovana Ferreira Moreira, Ilton Wellington de Sousa Ferreira, Ana Estéfanny Alves Cabral, Aparecida Marisa da Silva, Draenne Micarla dos Santos Silva, Felipe Lima de Medeiros, Mateus Medeiros de Oliveira, Myria Juscilania Maraço Silva, Myriam Juscilania Maraço Silva, Natallia de Oliveira Barros, Sheila Renata Roa Ovelar, Vanessa Amancio da Silva, Amanda Martins Fagundes, Caroline Eler Silva, Vicente Guimarães Fernandes Barcelos Martins, Daniela Camargos Costa, Murilo Elias Fronza, Andriele Sanfelice, Gabriel Fogaça Espírito Santo, Samara Quoos Da Rosa, Ana Clara Godinho Acauan, Juliana Gregol Sirtoli, Clara Diniz Machado Nunes, Bruna Santana Regattieri de Biase, Eduarda de Soares Libânio, Júlia Holer Naves Ribeiro, Gabriela Cunha Fialho Cantarelli Bastos, Rachel Daher Vieira Machado, Guilherme Briczinski de Souza, Carlos Daniel Vieira, David de Souza Mendes, Eduardo Sander Vieira, Geovana Pacheco, Marina Gonçalves Pozzobon, Nathália Vescia Bauer, Amanda Caroline da Silva Morais, MARCOS DIVINO DE OLIVEIRA JÚNIOR, Débora Borges de Oliveira Silva, Suiane Costa Ferreira, Angela Gabriela da Silva Santana, Bruna Barreto Mascarenhas, Lorrana Corina Gomes, Michael da Paixão Soares, Laís Martins da Costa Miranda, Beatriz Souza Sansoni, Camilla Melo Barbosa, Layla Pasolini Lott, Mariana Mesquita Zschaber, Paula Rodrigues de Magalhães, Priscilla Brunelli Pujatti, Ayslan Rodrigues de Paiva, Isabelle Teixeira Menezes, Geziany Vieira dos Santos Cunha, Isabella Cristina Santos de Castro, Lara Júlia Veríssimo Marra, Lorena Pereira de Sousa, Luiz Gustavo da Silva Prado, Pollyanna de Cárita Apolinário, Soraya de Souza Marques Leite, Wanessa Fleury Silva Graciano, Bernardo Valle Zanetti, Carolina Silva Miranda, Clarissa Nakakura, Diúle Nunes Sales, Giovanna Resende de Oliveira Lopes, Jade Luiza Ferreira Gonçalves, Josequeli Maria Goulart Oliveira, Kaio Alves Felipe, Karine Borges Ferreira, Lívia Nóbrega Ribeiro, Liz Ribeiro Faria, Maria Luisa Oliveira Almeida, Milla Giancristofaro Dutra, Rafaela Pereira Ribeiro, Luiza Oliveira Martins, Jhennifer Stephanye Venturato Vieira, Marina Pacheco Teles, Thayna Kathleen Pereira Martins de Paula, Priscilla Cardoso da Silva, Vanessa Dias Possamai, Lúcia Faria Borges, Andréa Kruger Gonçalves, Ana Luiza Bastos Magalhaes, Ana Júlia Queiroz Isaac Horbylon, Fernanda Gabriel Aires Saad, Arthur Kramer Neto, Gabriel Seroiska, Pedro Henrique Torres Tietz, Helena Guedes da Rocha, Grasiele do Amaral Martins, Carolina Guimarães Herzog, Caroline Engster da Silva, Matheus Ribeiro Fretes, Laís Bettoni, Rafaella Aléssio Naibo, Adriano Louro Moreira, Rafaela da Silva Cruz, Estefany Karenine Rodriguez Casanova, Guilherme Rodrigues Viana, Egídio Júnior Lorenzetti Ruggini, Georgia Marques Jardim, Marina de Araújo Teixeira, Bianca de Carvalho Pinheiro, Bruna Carreira Machado, Eduarda Penhalber, Fernanda Silva Ferrari, Giovanna Rossini Pires Baptista, Lara de Oliveira Trigo, Mariana Garcia Herrero Juliani, Rafaela Regis Berardo Cunha, Suzzy Caroline Meneghetti, Ronaldo Souza Piber, Leonardo de Souza Piber, Fabiane Weber Garcia, Leandro Rozin, Dailyt Salvador Guimarães, Stefany Carolinne Franco, Thaiane Oliveira de Lima, Ângela Aparecida Alves, Julia Deolinda Lustosa, Ana Laura da Silva Ferreira, Anangela Ravena da Silva Leal, Luan Victor Brandão dos Santos, Jéssica Missilany da Costa, Maria Fernanda Gomes do Nascimento, Vagner José Mendonça, Anna de Paula Freitas Borges, Júllia Santana Prudente de Angelis, Luiza Caroline Felicianno Sampaio, Mikaelly Rosy Borges Camillo, Renata Cordeiro dos Santos Rodrigues, Allana Victória Pereira Alves, Ana Raíssa de Melo Andrada Loureiro, Beatriz Bispo Lucas, Camila Oliveira de Figueiredo, Elizabeth Fechine Cruz, Fernanda Sampaio Feitosa Rocha, Felippe de Sousa Santos, Gabriel Teles de Souza Siqueira, Isadora Maysa de Souza, Juliana Viana Torres, Lara Cronemberger Cruz de Araújo, Maria Cecília Alencar de Amorim, Monna Myrelle Figueirêdo Golçalves, Sibely Coelho Urbano Pereira, Vanessa Pereira Corrêa, Vitória de Oliveira de Souza, Lisiane Tuon, Rafael Zaneripe de Souza Nunes, Larissa de Souza Mazzuco, Maria Júlia Colonetti Dário, Hexael Borges Demarch, Luciane Bisognin Ceretta, Renan Antonio Ceretta, Anibal Dário Jr, Luísa Carvalho, Debora do Amaral Teixeira, Yara de Oliveira Pena, Larissa da Silva Torres França, Lucas Coimbra Vale, Matheus Soprani Silva, Paloma Dornas de Castro, Milena de Oliveira Simões, Rebeca Tavares Dantas, Amanda Mikaele Andrade Furtado, Ana Clara Batista Cordeiro do Amaral, Graziela Elias Alves, Marco Antônio Vinciprova DallAgnese, Bianca B da Silva, Letícia Vieira Senger, Helena Marcon Bischoff, Pedro Dutra Batista, Alexandre Perin Decol, Mariana Moscon Gomes, Julia Guidoni Senra, Luiz Victor Crespo Barros, Emily Quintino Soares, Izabella Caser Lopes de Faria, Agnes Silva Campos, Ana Paula Kuster Lodi, Emmily Matias Pereira, Lara Altoé Bizzi, José Gobbi de Souza, Fernanda Cruz Carvalho, Bárbara Franccesca Brandalise Bassani, Andricely Barbosa Vidal, Júlia Bortolini Roehrig, Gabriela Uberti, Lana Caroline Palaver DallAgo, Larissa de Oliveira Silveira, Fernanda Pinheiro, Stefany Vieira Vidal, Lídia Carvalho dos Santos, Alice Wichrestiuk Darisbo, Lara Helena Zortéa, Ronaldo Gomes Silva, Kassia Regina dos Santos Cristaldo, Luana Maria Leite Villarim Dias, João Rafael Pereira Bezerra Cavalcanti, Bianca Brinques da Silva, Elaine Santana Gonçalves, Ana Caroline De Souza Martins, Jaqueline Roberta Cardoso Santos, Thais Helena Carvalho Correa, Vitoria Pereira Massote, Bárbara Azevedo Pereira Torres, Elizangela Guedes, Ranieri Sales de Souza Santos, Robson Ribeiro Rodrigues Filho, Luan Carlos Nunes Pinheiro, Davi Lima Lopes, Ilana Lima Lopes, Antônia Victoria Trindade Alves, Antônio Lucas Ferreira Alcântara, Emile Karen Gomes da Silva, Francisco Matheus Bezerra Silva, José Ary de Oliveira Abreu, Alan David Cavalcante Rabelo, Jaciara Honório Viana, Thayná Freitas de Araújo, Francisco Uelber Saraiva de Sousa, Luzia Mônica Almeida Cesar, Ana Carolina Miranda Gonçalves, Lara Carvalho de Oliveira, Natália Paulino Vilela Maciel, Laiane Santos Ferreira, Beatriz Essenfelder Borges, Nathan Dzivenka Vieira, Victor Tholken, Veridiana Gomes, Fernanda Maria de Souza, Gislaine Silveira de Lima, Giovanna Carstens Castellano, Rodrigo Otávio de Faria, Antonio Bruno Do N Rodrigues, Dathynara Da Silva Alves, Patrícia Flávia Costa Da Silva, Antonia Peres Silva, Célia Máximo De Barros Sousa, Maria Jakellyne Da Costa Sousa, Fernanda Maria Alves Sampaio, Thalia Maria de Sousa Soares, Rayane M M Monteiro, Kayra Cardoso Guimarães, Luana Patricia De Sousa Araújo, Juliana Maria Martins Pinheiro, Lidiana Matos Da Costa, Francisco Pereira de Miranda Júnior, Giovana da Rocha Leal Dias, Paulo Henrique Sousa Santos, Silmara Ferreira de Oliveira, Beatriz Sousa Santos, Nilsa Araújo Tajra, Caio Matheus Feitosa de Oliveira, Lucas A Ferreira, Fernanda Ribeiro da Silva, Rodrigo Peniche Lopes, Tinara LS Aarão, Márcia Guelma Santos Belfort, Cianny Ximenes Rodrigues Silva, Suellen alves de azevedo, Vanderlene brasil lucena, Whandra braga pinheiro abreu, Samara Raquel Dos Santos Lopes, Henrique Guimarães Vasconcelos, Mariana Kely Diniz Gomes de Lima, Dailyt Guimarães Salvador, Filippe de Sousa Santos, Laura de Souza Lima, and Anibal Dário J S

Published
2021

4. Opções reais em decisões estratégicas: análise de uma termelétrica 'Reservoir to Wire' sob a regulação brasileira vigente

Author

Suprani, Rodrigo Otávio de Faria, Escolas::EPGE, Sarmento, Joaquim Miranda, Souza, Rafael Martins de, and Gonçalves, Edson Daniel Lopes

Subjects
Sociedades comerciais - Finanças, Opções reais (Finanças), Opções reais, Processo decisório, Finanças corporativas, Finanças, Decisões de investimento, Economia, Investimentos - Brasil
Abstract

Este trabalho tem por objetivo aplicar a Análise de Opções Reais ao caso prático de uma usina termelétrica a gás natural que utiliza o modelo Reservoir to Wire (RTW), ou seja, que produz combustível próprio a ser utilizado na geração de energia elétrica. Devido à regulação brasileira, a empresa é obrigada a atender à demanda do Sistema Interligado Nacional (SIN) e, por isso, se expõe a um grave risco regulatório de multas e sanções caso decida por uma menor capacidade de produção de gás natural e, consequentemente, por menor disponibilidade de combustível para a geração. O modelo deste trabalho parte do valuation de um projeto de pesquisa e desenvolvimento do Centro de Estudos em Regulação e Infraestrutura (CERI-FGV), no qual foi calculado um despacho mensal esperado de energia para dimensionar o investimento. Com as propriedades estatísticas dos despachos, foram realizadas análises de Monte Carlo que permitem construir a árvore de decisão de opções reais que incorpora dois regimes de volatilidade de retornos do projeto: um com capacidade apenas suficiente para o despacho mensal esperado e outro com capacidade total de produção de gás natural. O trabalho conclui que a opção real em análise se trata de uma opção única limitada a um único exercício. A empresa pode se expor ao risco e limitar o investimento no momento inicial, ampliando a capacidade de produção de gás caso se tornem mais prováveis os cenários menos favoráveis, com um gerenciamento ativo de riscos que adiciona valor ao projeto. This work aims at applying the Real Options Analysis for the practical case of a natural gas thermoelectric power plant that uses the Reservoir to Wire (RTW) model i.e. that produces its own fuel to be used in electric power generation. Due to the Brazilian regulation, the company is enforced to meet the demand from the National Interconnected System (SIN) and is therefore exposed to a grave regulatory risk of fines and sanctions in case it may decide for a lower capacity for natural gas production and hence for a lower availability of fuel for generation. This work’s model begins with a valuation model from a research and development project from the Center for Regulation and Infrastructure Studies (CERI-FGV), in which an expected monthly energy dispatch was calculated to assess the investment amount. With the statistical properties of the dispatches, Monte Carlo analyses were realized to build the real option decision tree that includes two schemes for the volatility of the project’s returns: one with just enough capacity to the expected monthly dispatch and another at full capacity for natural gas production. The work concludes that the case’s real option is a one-time only option that is limited to a single exercise. The company may remain exposed to the risk and limit the investment amount at the present time, expanding the production capacity of natural gas in case the less favorable scenarios become more likely, with an active risk management that adds value to the project.

Published
2019

5. Exopolysaccharide from surface-liquid culture of Clonostachys rosea originates from autolysis of the biomass

Author

Guilherme L. Sassaki, Regielly Caroline Raimundo Cognialli, Rodrigo Otávio de Faria, Elaine R. Carbonero, David A. Mitchell, Graciele Viccini, and Thalita Romano Martinelli

Subjects
Autolysis (biology), Biological pest control, Fungus, Polysaccharide, Biochemistry, Microbiology, Mannans, Galactomannan, chemistry.chemical_compound, Microbial ecology, Cell Wall, Botany, Genetics, Bionectriaceae, Biomass, skin and connective tissue diseases, Molecular Biology, chemistry.chemical_classification, biology, Galactose, General Medicine, Fungi imperfecti, bacterial infections and mycoses, biology.organism_classification, Culture Media, carbohydrates (lipids), Glucose, chemistry, Fermentation, Hypocreales, Autolysis
Abstract

We describe the purification and chemical characterization of galactomannans that appear both in the biomass and the culture broth during surface-liquid culture of the fungus Clonostachys rosea, a common facultative saprophyte that has potential to be used as a biological control agent against several plant pathogenic fungi, insects and nematodes. The galactomannans from both sources had comparable ratios of Man, Gal and Glc and the similarity were confirmed by (1)H, (13)C NMR, HMQC, and COSY spectra. We propose that the galactomannan in the culture broth originates from autolysis of the biomass, based not only on the similarity that it has with the galactomannan extracted from the biomass but also on the fact that its concentration increased rapidly after glucose depletion from the medium, when biomass concentration was falling. Polysaccharides from C. rosea have not previously been characterized; we show that the characteristics of the galactomannans are consistent with those that have been reported for other members of the Bionectriaceae, the family to which C. rosea belongs.

Published
2009

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