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RESUMEN Antecedentes: La estructura vegetal de los cafetales con sombra ha funcionado como nicho de la biota nativa. Entre esta biota destacan los hongos micorrízicos arbusculares que forman simbiosis con las plantas y liberan una glicoproteína llamada glomalina que contribuye a la reserva de carbono en los suelos. Objetivo: Caracterizar los niveles de las fracciones de proteínas del suelo relacionadas a glomalina total y fácilmente extraíble y analizar su relación con el número de esporas de los hongos micorrízicos arbusculares y con algunas características fisicoquímicas de los suelos cafetaleros. Métodos: Se analizaron suelos de cinco cafetales bajo sombra y se utilizaron diferentes métodos extractivos, seguido de la estimación de la concentración de proteínas para la determinación de glomalina y un conteo de esporas de los hongos micorrízicos arbusculares. Resultados y discusión: Los valores de glomalina fácilmente extraíble y total oscilaron entre 0.15-a 0.46 y 0.57-2.03 mg/kg respectivamente. La finca Jilotepec1 presentó los mayores valores de las dos fracciones de glomalina. Las regresiones lineales revelaron una relación significativa positiva entre la glomalina total con la materia orgánica, con el carbono orgánico, con el nitrógeno y carbono total. Se observó una relación significativa negativa de la glomalina total con el fósforo disponible y la densidad aparente del suelo. No obstante que no se observó una relación significativa entre la glomalina con el número de esporas, se denota una tendencia, de tal manera que es factible utilizar la medición de glomalina para cuantificar la actividad de los hongos micorrízicos arbusculares en las fincas cafetaleras. ABSTRACT Background: The vegetal structure of coffee plantation under shadow has functioned as niche of native biota. Among this biota, its highlight arbuscular mycorrhizal fungi that form symbiosis with these plants and release a glycoprotein named glomalin which contributes to the carbon reserve in soils. Objective: To characterize the levels of the fractions of glomalin-related soil proteins and easy extractable and their relationship with the number of arbuscular mycorrhizal fungi spores and several physic-chemical characteristics of coffee soils. Methods: Soils of five coffee plantations under shadow with the same management were analyzed. Different extractive methods were used, followed by the protein-concentration estimation to determine glomalin and the arbuscular mycorrhizal fungi spores counting was achieved. Results and discussion: Easily extractable and total glomalin values ranged from 0.15-0.46 and 0.57-2.03 mg/kg, respectively. The Jilotepec1 coffee plantation presented the highest values of the two glomalin fractions. Linear regressions revealed a significant positive relationship between total glomalin with organic matter, organic carbon, and total nitrogen and carbon. A significant negative relationship of total glomalin with soil available phosphorus and soil bulk density. Although a significant relationship between glomalin and the number of spores was not observed, a trend is denoted, in such a way that glomalin can be used to quantify the activity of arbuscular mycorrhizal fungi in coffee plantations., {"references":["SADER. Obtenido https://www.gob.mx/agricultura","Moguel P, Toledo VM. Biodiversity conservation and traditional coffee systems of Mexico. Biol Conserv 1999; 13:11-21.","Cerdán CR, Rebolledo MC, Soto G, Rapidel B, Sinclair FL. Local knowledge of impacts of tree cover on ecosystem services in smallholder coffee production systems. Agric Syst 2012; 110:119-130. doi:10.1016/j.agsy.2012.03.014","Chait G. Café en Colombia: servicios ecosistémicos, conservación de la biodiversidad. 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