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2 results on '"Rodríguez Mayusa, Jeanet"'

1. NMR spectroscopy and antioxidant activity of flavanones and flavones isolated from Chromolaena tacotana (Klatt) R.M. King & H. Rob

Author

Torrenegra Guerrero, Rubén, Bautista Bautista, L, Rodríguez Mayusa, Jeanet, and Méndez Callejas, Gina

Subjects
Radicales libres, Chromolaena, Flavonas, Flavonoides
Abstract

Chromolaena tacotana is considered as a source of flavonoids. Here we examined the content and antioxidant properties of flavones and flavanones from the leaves of the plant. Four flavonoids, including (Cta) 5, 4' dihydroxy-7-methoxy flavanone, (Ctb) 3,5,3'-trihydroxy-7,4'-dimethoxyflavone; (Ctc), 3,4'-dihydroxy -5,7-dimethoxyflavanone; and (Ctd) 4'-hydroxy-5,7-dimethoxyflavanone, were isolated from leaves extracts, were identified by their NMR spectroscopic data, and then free radical scavenging activities of the flavonoids were assessed against DPPH. The antioxidant activity for the flavanone Ctb was the highest even compared to that of quercetin, with IC50 of 6.27 μg/mL and 8.67 μg/mL respectively. The flavanones Cta, Ctc and Ctd presented a lowest activity against free radicals as expected according to their molecular substituents and the position within the structure. Data obtained from this study support the ethnomedicinal use of the leaves of C. tacotana for an antioxidant purpose.

Published
2020

2. Antiproliferative activity of chloroformic fractions from leaves and inflorescences of Ageratina gracilis.

Author

Méndez-Callejas, Gina, Rodríguez-Mayusa, Jeanet, Riveros-Quiroga, Myriam, Mahete-Pinilla, Kelly, and Torrenegra-Guerrero, Rubén

Subjects
*INFLORESCENCES, *DOMESTICATION of plants, *ANTINEOPLASTIC agents, *ANTIBODY-dependent cell cytotoxicity, *NEOPLASTIC cell transformation
Abstract

To obtain a scientific basis and justification of plant domestication in the use of Ageratina gracilis, we did an in vitro study of the anticancer potential of extracts and fractions from its leaves and inflorescences. Firstly, cytotoxicity was evaluated against five human tumorigenic cell lines by MTT assay. Subsequently, the chloroformic fractions, considered the most cytotoxic were tested for genotoxicity by comet assay, morphological effects were analyzed by fluorescent microscopy, cell cycle arrest by flow cytometry and early apoptosis induction through fluorescein-5-isothiocyanate (FITC) labeled Annexin-V assay. Non-polar extracts with IC50 values of <53µg/ml showed a high cytotoxicity. The highest cytotoxicity was achieved by chloroformic fraction from petroleum ether extract of leaves and inflorescences and chloroformic fraction from ethanolic extract of leaves, displaying a significant inhibition of cell viability particularly on A549 cells with an IC50 value of 25.9 µg/mL. Chloroformic fractions caused a high percent of DNA damage above 60 percent on A549 and MDAMB-231.The fractions also induced G1/S phase arrest of the cell cycle in A549 cells, furthermore it was confirmed the apoptotic activity chloroformic fraction from petroleum ether extract of inflorescences and chloroformic fraction from ethanolic extract of leaves on those cells by Annexin-V assay. These preliminary results indicate that A. gracilis has an antiproliferative activity against cancer cells, being a starting point for forthcoming studies about the antineoplastic activity and its domestication conditions. [ABSTRACT FROM AUTHOR]

Published
2017
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