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237 results on '"Rodpai, Rutchanee"'

14. Improved diagnostic sensitivity of human strongyloidiasis using point-of-care mixed recombinant antigen-based immunochromatography

Author

Boonroumkaew Patcharaporn, Sadaow Lakkhana, Janwan Penchom, Rodpai Rutchanee, Sanpool Oranuch, Buadee Punyisa, Suprom Chanida, Thanchomnang Tongjit, Intapan Pewpan M., and Maleewong Wanchai

Subjects
strongyloidiasis, neglected tropical disease, recombinant antigen, point-of-care, serodiagnosis, immunochromatography test, Infectious and parasitic diseases, RC109-216
Abstract

Strongyloidiasis is a neglected tropical disease that can cause fatal complications due to hyperinfection and disseminated strongyloidiasis in immunocompromised patients. We used two Strongyloides stercoralis recombinant antigenic proteins, L3NieAg.01 (NIE) and IgG-immunoreactive antigen (SsIR), to develop the recombinant antigen-based immunochromatography test (ICT) kit. We constructed and compared kits using either the NIE (NIE ICT kit) or the SsIR (SsIR ICT kit) antigens and a kit using a mixture of both (NIE-SsIR ICT kit) for detection of anti-Strongyloides IgG antibody in human serum samples. Serum samples from normal healthy individuals (Group I, n = 40), proven strongyloidiasis patients (Group II, n = 100), and those with other parasitic infections (Group III, n = 154) were evaluated. Sensitivity and specificity were 81.0% and 84.0% for the NIE ICT kit, 89.0% and 83.5% for the SsIR ICT kit, and 95.0% and 90.2% for the NIE-SsIR ICT kit, respectively. The NIE-SsIR ICT kit provided the best diagnostic results; it can supplement stool examination for clinical diagnosis and can be used to screen for asymptomatic S. stercoralis infection in people at risk in endemic areas. The NIE-SsIR ICT kit can also be used in large-scale sero-epidemiological investigations in endemic areas without the need for additional facilities or ancillary supplies.

Published
2023
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19. Bacterial community structure of Anopheles hyrcanus group, Anopheles nivipes, Anopheles philippinensis, and Anopheles vagus from a malaria-endemic area in Thailand

Author

Boonroumkaew, Patcharaporn, primary, Rodpai, Rutchanee, additional, Saeung, Atiporn, additional, Aupalee, Kittipat, additional, Saingamsook, Jassada, additional, Poolphol, Petchaboon, additional, Sadaow, Lakkhana, additional, Sanpool, Oranuch, additional, Janwan, Penchom, additional, Thanchomnang, Tongjit, additional, Intapan, Pewpan M., additional, and Maleewong, Wanchai, additional

Published
2023
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20. Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, Schistosoma mansoni

Author

Ittiprasert, Wannaporn, primary, Moescheid, Max F., additional, Chaparro, Cristian, additional, Mann, Victoria H., additional, Quack, Thomas, additional, Rodpai, Rutchanee, additional, Miller, André, additional, Wisitpongpun, Prapakorn, additional, Buakaew, Watunyoo, additional, Mentink-Kane, Margaret, additional, Schmid, Sarah, additional, Popratiloff, Anastas, additional, Grevelding, Christoph G., additional, Grunau, Christoph, additional, and Brindley, Paul J., additional

Published
2023
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24. Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, Schistosoma mansoni

Author

Ittiprasert, Wannaporn, Moescheid, Max F., Chaparro, Cristian, Mann, Victoria H., Quack, Thomas, Rodpai, Rutchanee, Miller, André, Wisitpongpun, Prapakorn, Buakaew, Watunyoo, Mentink-kane, Margaret, Schmid, Sarah, Popratiloff, Anastas, Grevelding, Christoph G., Grunau, Christoph, Brindley, Paul J., Ittiprasert, Wannaporn, Moescheid, Max F., Chaparro, Cristian, Mann, Victoria H., Quack, Thomas, Rodpai, Rutchanee, Miller, André, Wisitpongpun, Prapakorn, Buakaew, Watunyoo, Mentink-kane, Margaret, Schmid, Sarah, Popratiloff, Anastas, Grevelding, Christoph G., Grunau, Christoph, and Brindley, Paul J.

Abstract

The identification and characterization of genomic safe harbor sites (GSHs) can facilitate consistent transgene activity with minimal disruption to the host cell genome. We combined computational genome annotation and chromatin structure analysis to predict the location of four GSHs in the human blood fluke, Schistosoma mansoni, a major infectious pathogen of the tropics. A transgene was introduced via CRISPR-Cas-assisted homology-directed repair into one of the GSHs in the egg of the parasite. Gene editing efficiencies of 24% and transgene-encoded fluorescence of 75% of gene-edited schistosome eggs were observed. The approach advances functional genomics for schistosomes by providing a tractable path for generating transgenics using homology-directed, repair-catalyzed transgene insertion. We also suggest that this work will serve as a roadmap for the development of similar approaches in helminths more broadly.

Published
2023
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28. Prevalence of intestinal parasitic infections and genetic differentiation of Strongyloides stercoralis among migrant workers from Myanmar, Lao PDR and Cambodia in northeastern Thailand

Author

Sangwalee, Wararat, primary, Norkaew, Jun, additional, Inthachak, Sengchoy, additional, Janwan, Penchom, additional, Rodpai, Rutchanee, additional, Sanpool, Oranuch, additional, Sadaow, Lakkhana, additional, Boonroumkaew, Patcharaporn, additional, Intapan, Pewpan M., additional, Maleewong, Wanchai, additional, and Thanchomnang, Tongjit, additional

Published
2022
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33. Evaluation of total immunoglobulin G and subclass antibodies in an enzyme-linked immunosorbent assay for serodiagnosis of human amebic liver abscess

Author

Janwan, Penchom, primary, Sadaow, Lakkhana, additional, Rodpai, Rutchanee, additional, Yamasaki, Hiroshi, additional, Luvira, Vor, additional, Sukeepaisarnjaroen, Wattana, additional, Kitkhuandee, Amnat, additional, Paonariang, Krisada, additional, Sanpool, Oranuch, additional, Boonroumkaew, Patcharaporn, additional, Thanchomnang, Tongjit, additional, Mita, Toshihiro, additional, Intapan, Pewpan M., additional, and Maleewong, Wanchai, additional

Published
2022
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34. Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke,Schistosoma mansoni

Author

Ittiprasert, Wannaporn, primary, Moescheid, Max F., additional, Chaparro, Cristian, additional, Mann, Victoria H., additional, Quack, Thomas, additional, Rodpai, Rutchanee, additional, Miller, André, additional, Wistiphongpun, Prapakorn, additional, Buakaew, Watunyoo, additional, Mentink-Kane, Margaret, additional, Schmid, Sarah, additional, Popratiloff, Anastas, additional, Grevelding, Christoph G., additional, Grunau, Christoph, additional, and Brindley, Paul J., additional

Published
2022
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37. First molecular identification and report of genetic diversity of Strongyloides stercoralis, a current major soil-transmitted helminth in humans from Lao People’s Democratic Republic

Author

Laymanivong, Sakhone, Hangvanthong, Bouasy, Insisiengmay, Bounnaloth, Vanisaveth, Viengxay, Laxachack, Pinnakhone, Jongthawin, Jurairat, Sanpool, Oranuch, Thanchomnang, Tongjit, Sadaow, Lakkhana, Phosuk, Issarapong, Rodpai, Rutchanee, Maleewong, Wanchai, and Intapan, Pewpan M.

Published
2016
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39. Evaluation of total immunoglobulin G and subclass antibodies in an enzymelinked immunosorbent assay for serodiagnosis of human amebic liver abscess.

Author

Janwan, Penchom, Sadaow, Lakkhana, Rodpai, Rutchanee, Hiroshi Yamasaki, Luvira, Vor, Sukeepaisarnjaroen, Wattana, Kitkhuandee, Amnat, Paonariang, Krisada, Sanpool, Oranuch, Boonroumkaew, Patcharaporn, Thanchomnang, Tongjit, Toshihiro Mita, Intapan, Pewpan M., and Wanchai Maleewong

Subjects
LIVER abscesses, IMMUNOGLOBULIN G, SERODIAGNOSIS, IMMUNOGLOBULINS, ENZYME-linked immunosorbent assay, ENTAMOEBA histolytica, PARASITIC diseases
Abstract

Background. Amebic liver abscess (ALA) caused by Entamoeba histolytica is usually diagnosed based on its clinical symptoms, medical imaging abnormalities of the liver, and serological tests, the most common being the enzyme-linked immunosorbent assay (ELISA). For more than three decades, no investigation has evaluated the diagnostic performance of immunoglobulin G (IgG) subclasses in the serodiagnosis of ALA. Herein, we assessed the efficiencies of anti-amebic IgG and IgG subclasses for diagnosing ALA. Methods. A serological ELISA-based test was performed to assess its diagnostic performance using a total of 330 serum samples from ALA patients (nD14), healthy individuals (nD40), and patients with other diseases (nD276). Results. ELISA targeting the total IgG antibody to E. histolytica antigen exhibited 100% sensitivity 95% CI [76.8-100.0] and 97.8% specificity 95% CI [95.5-99.1], whereas the assay targeting IgG1 showed the same sensitivity (100% 95% CI [76.8-100.0]) and a slightly higher specificity (99.1% 95% CI [97.3-99.8]). The other IgG subclasses (IgG2, IgG3, and IgG4) displayed a lower sensitivity and specificity. The sensitivity and specificity did not significantly differ between tests measuring total IgG and IgG1 (Exact McNemar's test; p > 0.05), with a concordance of 98.2%, represented by a Cohen's kappa of 0.83 (p < 0.001), indicating almost perfect agreement. Conclusion. ELISA targeting IgG1 can provide valuable information to clinicians in differentiating ALA from other parasitic diseases, cancers, cirrhosis, and viral hepatitis. However, enzyme-conjugated anti-human total IgG is cheaper than anti-human IgG subclasses. Therefore, we suggest that total IgG-based ELISA is sufficient for the routine serodiagnosis of human ALA and possibly other clinical manifestations of invasive amebiasis. [ABSTRACT FROM AUTHOR]

Published
2022
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40. Exposure to dexamethasone modifies transcriptomic responses of free-living stages of Strongyloides stercoralis

Author

Rodpai, Rutchanee, primary, Sanpool, Oranuch, additional, Thanchomnang, Tongjit, additional, Laoraksawong, Pokkamol, additional, Sadaow, Lakkhana, additional, Boonroumkaew, Patcharaporn, additional, Wangwiwatsin, Arporn, additional, Wongkham, Chaisiri, additional, Laummaunwai, Porntip, additional, Ittiprasert, Wannaporn, additional, Brindley, Paul J., additional, Intapan, Pewpan M., additional, and Maleewong, Wanchai, additional

Published
2021
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42. Development of Immunochromatographic Test Kit for Rapid Detection of Specific IgG4 Antibody in Whole-Blood Samples for Diagnosis of Human Gnathostomiasis

Author

Janwan, Penchom, primary, Intapan, Pewpan M., additional, Sadaow, Lakkhana, additional, Rodpai, Rutchanee, additional, Yamasaki, Hiroshi, additional, Boonroumkaew, Patcharaporn, additional, Sanpool, Oranuch, additional, Thanchomnang, Tongjit, additional, Sadee, Phuangphaka, additional, and Maleewong, Wanchai, additional

Published
2021
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43. High prevalence of opisthorchiasis in rural populations from Khammouane Province, central Lao PDR: serological screening using total IgG- and IgG4-based ELISA

Author

Phupiewkham, Weeraya, primary, Rodpai, Rutchanee, additional, Inthavongsack, Somchith, additional, Laymanivong, Sakhone, additional, Thanchomnang, Tongjit, additional, Sadaow, Lakkhana, additional, Boonroumkaew, Patcharaporn, additional, Sanpool, Oranuch, additional, Janwan, Penchom, additional, Intapan, Pewpan M, additional, and Maleewong, Wanchai, additional

Published
2021
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44. Diminished adherence of snail hemocytes to schistosome sporocysts of Schistosoma mansoni following programmed knockout of the allograft inflammatory factor of Biomphalaria glabrata

Author

Coelho, Fernanda Sales, Rodpai, Rutchanee, Miller, André, Karinshak, Shannon E, Mann, Victoria H, Carvalho, Omar dos Santos, Caldeira, Roberta Lima, de Moraes Mourão, Marina, Brindley, Paul J, and Ittiprasert, Wannaporn

Abstract

Schistosomiasis mansoni is a debilitating neglected tropical disease caused by infection with the blood fluke, Schistosoma mansoni . Development of the larval stage of the parasite in an intermediate host snail of the genus Biomphalaria is an obligatory component of the life cycle. Enhanced understanding of the mechanism(s) of host defense in the intermediate host snail may hasten the development of new tools that block transmission of schistosomiasis. The embryonic cell line from B. glabrata , termed the Bge line, is a versatile resource in the investigation of snail-schistosome interactions. A key attribute of the Bge cell is its hemocyte-like phenotype, given the central role of the snail hemocyte in innate and cellular immunity. The allograft inflammatory factor 1 (AIF, aif ) is a conserved antigen typically expressed in phagocytic and granular leukocytes and is a marker of macrophage activation. AIF is highly expressed in isolates of B. glabrata that are resistant to infection with S. mansoni . We targeted the aif gene of B. glabrata ( Bg AIF) for programmed gene knockout using the CRISPR/Cas9 approach, to investigate the activity of CRISPR/Cas9 gene editing in this gastropod species and, in turn, to investigate the loss of AIF on Bge cell-mediated adherence to schistosome sporocysts. Bge cells were transformed with gene editing plasmid encoding the Cas9 nuclease driven by the CMV promoter and a guide RNA specific for exon 4 of the Bg AIF by square wave electroporation. Twelve independent transformations were carried out. Transcript levels of Bg AIF were significantly reduced by up to 73% (range, 26 to 73%; mean 49.5±20.2% S.D; n = 12) from two to nine days following transformation. Sanger direct sequencing and bioinformatic analysis of the sequence reads revealed on-target programmed mutation on the Bg AIF gene, including insertions of 1-2 bp and deletion of 8-30 bp, (range, 9 to 17% of Bg AIF gene; n =12). In addition, the adherence of gene-edited Bge cells to sporocysts was significantly impeded in comparison to control cells, as ascertained using the cell adherence index (CAI); 2.66±0.10 (control) vs . 2.30±0.22 (Δ Bg AIF cells), P < 0.05. This is the first report of gene editing in this medically important taxon of freshwater gastropods that are necessary for the transmission of schistosomiasis.

Published
2020
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47. Diminished adherence of Biomphalaria glabrata embryonic cell line to sporocysts of Schistosoma mansoni following programmed knockout of the allograft inflammatory factor

Author

Coelho, Fernanda Sales, primary, Rodpai, Rutchanee, additional, Miller, Andre, additional, Karinshak, Shannon E, additional, Mann, Victoria H, additional, Carvalho, Omar dos Santos, additional, Cladeira, Roberta Lima, additional, Mourao, Marina de Moraes, additional, Brindley, Paul J, additional, and Ittiprasert, Wannaporn, additional

Published
2020
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48. Effectiveness of Strongyloides Recombinant IgG Immunoreactive Antigen in Detecting IgG and IgG4 Subclass Antibodies for Diagnosis of Human Strongyloidiasis Using Rapid Immunochromatographic Tests

Author

Boonroumkaew, Patcharaporn, primary, Sadaow, Lakkhana, additional, Sanpool, Oranuch, additional, Rodpai, Rutchanee, additional, Thanchomnang, Tongjit, additional, Phupiewkham, Weeraya, additional, Intapan, Pewpan M., additional, and Maleewong, Wanchai, additional

Published
2020
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50. Development and Accuracy Evaluation of Lateral Flow Immunoassay for Rapid Diagnosis of Schistosomiasis Mekongi in Humans.

Author

Rodpai, Rutchanee, Sadaow, Lakkhana, Sanpool, Oranuch, Boonroumkaew, Patcharaporn, Thanchomnang, Tongjit, Laymanivong, Sakhone, Janwan, Penchom, Limpanont, Yanin, Chusongsang, Phiraphol, Ohmae, Hiroshi, Yamasaki, Hiroshi, Lv, Zhiyue, Intapan, Pewpan M., and Maleewong, Wanchai

Subjects
*SCHISTOSOMIASIS, *IMMUNOASSAY, *ENDEMIC diseases, *DIAGNOSIS, *DISEASE prevalence, *TRICHINOSIS
Abstract

Schistosoma mekongi infection is endemic in countries along the Mekong River and certain of its tributaries in the lower Mekong basin, especially in Lao People's Democratic Republic and Cambodia. Diagnosis of schistosomiasis is crucial before treatment and epidemiological surveys before and/or after an intervention, such as a mass drug administration. A newly developed immunochromatographic test (ICT) for the diagnosis of schistosomiasis mekongi, based on antiparasite antibody detection in human sera, was evaluated. The schistosomiasis mekongi-ICT (Smk-ICT) strip was developed using somatic antigen from adult S. mekongi. In total, 209 serum samples were examined, including 14 from parasitologically proven schistosomiasis mekongi patients, 30 from schistosomiasis japonica patients, other parasitosis (n = 135), and healthy volunteers (n = 30) from areas not endemic for S. mekongi. Eleven schistosomiasis mekongi samples were positive according to the Smk-ICT, whereas all healthy control samples were negative. Cross-reactions with paragonimiasis heterotremus, sparganosis, trichinellosis, and taeniasis saginata samples were observed at 2.4% (4/165). The diagnostic sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were 78.6% (95% confidence interval [CI] 49.2–95.3), 97.6% (95% CI 93.9–99.3), 73.3% (95% CI 44.9–92.2), 98.2% (95% CI 94.7–99.6), and 96.1% (95% CI 92.1–98.4), respectively. The Smk-ICT kit might be useful to assess the prevalence of disease before establishing transmission control and mass deworming campaigns in countries in the Mekong River subregion. [ABSTRACT FROM AUTHOR]

Published
2022
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