36 results on '"Roczniak W"'
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2. Parasite eggs on dogs' and cats' hair,Jaja pasozytów na włosach psów i kotów
- Author
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Sadzikowski, A. B., KRZYSZTOF TOMCZUK, Studzińska, M. B., Witkowska, A., Roczniak, W., and Wasak, A.
3. Naturally Occurring High-Chloride Coal and Superheater Corrosion—A Laboratory Study
- Author
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Plumley, A. L., primary and Roczniak, W. R., additional
- Published
- 1982
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4. Evaluation of boiler-tube materials for advanced power cycles
- Author
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Roczniak, W
- Published
- 1979
5. Naturally occurring high-chloride coal and superheater corrosion - a laboratory study
- Author
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Roczniak, W
- Published
- 1982
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6. Cofiring of refuse-derived fuel and coal at Oak Creek. Volume 2. Boiler slagging and fouling evaluation. Final report
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Roczniak, W
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- 1982
7. Cofiring of refuse-derived fuel and coal at Oak Creek. Volume 1. Boiler corrosion evaluation. Final report
- Author
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Roczniak, W
- Published
- 1981
8. Nutritional Status and Selected Adipokines in Children with Irritable Bowel Syndrome.
- Author
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Roczniak W, Szymlak A, Mazur B, Chobot A, Stojewska M, and Oświęcimska J
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- Child, Female, Humans, Male, Adipokines, Adiponectin, Chemokines, Cytokines, Intercellular Signaling Peptides and Proteins, Leptin, Nutritional Status, Child, Preschool, Adolescent, Insulin Resistance, Irritable Bowel Syndrome
- Abstract
Background: The aim of this study was to assess the nutritional status and serum concentrations of adipokines in children with irritable bowel syndrome (IBS) and healthy controls. We also sought to evaluate their relation to metabolic parameters., Methods: We studied 33 IBS patients (11 girls, 22 boys) aged 5-17 years and 30 healthy age-matched controls (11 girls, 19 boys). The analysis included anthropometric measurements, body composition parameter measurements using bioimpedance, and biochemical tests and measurements of serum concentrations of leptin, adiponectin, chemerin, and omentin-1., Results: The results of the anthropometric measurements were comparable between the patients and the controls. The patients had higher triglycerides, HOMA-IRs, and chemerin concentrations than the healthy subjects. The HDL cholesterol and omentin-1 levels were lower than in the controls. Leptin and adiponectin did not differ significantly between the groups. An analysis of the receiver operator curves (ROCs) showed that serum concentrations of chemerin ≥ 232.8 ng/mL had 30% sensitivity and 87% specificity when they were used to differentiate between children with IBS and healthy subjects. In the case of serum omentin-1 concentrations ≤ 279.4 ng/mL, the sensitivity and specificity were 60% and 80%, respectively., Conclusions: The nutritional status of children with IBS did not differ from that of the healthy controls. We found significant differences in serum chemerin and omentin-1 concentrations between IBS patients and healthy children. These adipokines could be used as IBS biomarkers as they demonstrate good specificity and moderate sensitivity. The serum concentrations of chemerin and omentin-1 in IBS patients were related to nutritional status and insulin resistance.
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- 2022
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9. Tentative Assessment of Treatment of Supracondylar Humerus Fractures in Children.
- Author
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Kierońska A, Oliwko E, Babuśka-Roczniak M, Brodziak-Dopierała B, Widuchowski W, and Roczniak W
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- Bone Wires, Child, Fracture Fixation, Internal methods, Humans, Humerus, Elbow Joint surgery, Humeral Fractures surgery
- Abstract
Background: Supracondylar humerus fractures are a serious problem in children. The complicated anatomy of the elbow joint necessitates careful assessment of the damage before individually adapting treatment methods. The aim of the study was to evaluate the effectiveness of comprehensive treatment of children in Group A in relation to children in Group B. Moreover, the impact of rehabilitation on the function of the affected elbow joint was examined., Materials and Methods: The study enrolled 40 children divided into two groups: Group A and Group B. In Group A, the dominant method of treatment was percutaneous K-wire stabilization. The results demonstrate that the use of this treatment method and the implementation of systematic rehabilitation made it possible to achieve very good results. In Group B, stabilization was performed in less than half of the children and rehabilitation was carried out at home. The initial and follow-up examinations were carried out in both groups according to a medical test card. In Group A, the initial examination was performed on the day the rehabilitation commenced, and the follow-up examination after the completion of a 10-day rehabilitation cycle. In Group B, the initial examination was carried out after immobilization was removed and a follow-up examination was performed after 3 weeks of home-based rehabilitation., Results: The treatment model used in Group A was more effective than the model used in Group B., Conclusions: 1. Correct repositioning, the use of percutaneous K-wire stabilization and elbow rehabilitation performed at the earliest possible time give very good functional results. 2. The use of percutaneous stabilization shortens the hospitalization period, which is of great importance in the treatment of young patients.
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- 2022
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10. The Effect of the Ketogenic Diet on Adiponectin, Omentin and Vaspin in Children with Drug-Resistant Epilepsy.
- Author
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Chyra M, Roczniak W, Świętochowska E, Dudzińska M, and Oświęcimska J
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- Body Mass Index, Child, Child, Preschool, GPI-Linked Proteins metabolism, Humans, Insulin Resistance, Obesity, Adiponectin metabolism, Cytokines metabolism, Diet, Ketogenic, Drug Resistant Epilepsy diet therapy, Drug Resistant Epilepsy metabolism, Lectins metabolism, Serpins metabolism
- Abstract
Background: Changes in adipokine secretion may be involved in the anti-epileptic effect of a ketogenic diet (KD) in drug-resistant epilepsy (DRE)., Objectives: The assessment of the influence of KD on serum adiponectin, omentin-1, and vaspin in children with DRE., Methods: Anthropometric measurements (weight, height, BMI, and waist-to-hip circumference ratio) were performed in 72 children aged 3-9 years, divided into 3 groups: 24 children with DRE treated with KD, 26-treated with valproic acid (VPA), and a control group of 22 children. Biochemical tests included fasting glucose, insulin, beta-hydroxybutyric acid, lipid profile, aminotransferases activities, and blood gasometry. Serum levels of adiponectin, omentin-1 and vaspin were assayed using commercially available ELISA tests., Results: Serum levels of adiponectin and omentin-1 in the KD group were significantly higher and vaspin-lower in comparison to patients receiving VPA and the control group. In all examined children, serum adiponectin and omentin-1 correlated negatively with WHR and serum triglycerides, insulin, fasting glucose, and HOMA-IR. Vaspin levels correlated negatively with serum triglycerides and positively with body weight, BMI, fasting glucose, insulin, and HOMA-IR., Conclusion: One of the potential mechanisms of KD in children with drug-resistant epilepsy may be a modulation of metabolically beneficial and anti-inflammatory adipokine levels.
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- 2022
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11. Exocrine Pancreatic Function in Girls with Anorexia Nervosa.
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Malczyk Ż, Roczniak W, Mazur B, Kwiecień J, Ziora K, Górska-Flak K, and Oświęcimska J
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- Adolescent, Breath Tests, Carbon Dioxide analysis, Case-Control Studies, Child, Female, Ghrelin blood, Humans, Leptin blood, Receptors, Leptin blood, Triglycerides analysis, Triglycerides metabolism, Anorexia Nervosa physiopathology, Pancreas, Exocrine physiopathology
- Abstract
Objectives: To assess pancreatic exocrine function in patients with anorexia nervosa using a breath test with
13 C-labeled mixed triglycerides (MTG-BT) and to determine the relationship between the test results and selected biochemical and hormonal parameters., Material and Methods: Anthropometric measurements, biochemical and hormonal parameters (serum leptin, soluble leptin receptor (sLR), acylated and desacylated ghrelin, free leptin index (FLI)), and MTG-BT were performed in a group of 31 girls with the restrictive type of AN, as well as 38 healthy girls (C)., Results: The average cumulative dose of13 C-triglycerides recovered with exhaled air (%CD) was similar in both study groups, while the average time from13 C-triglycerides administration to peak13 CO2 excretion in expired air (time to peak (TTP)) was significantly longer in patients with AN compared to C. In both groups, %CD correlated negatively with FLI. TTP correlated negatively with sLR and FLI in the AN and with serum insulin and HOMA-IR values in the C., Conclusions: In girls with AN, the pancreatic efficiency of lipase secretion was found to be normal, while the kinetics of this enzyme secretion were disturbed. These changes may result from disorders in the functioning of the adipose-insular and islet-acinar axes.- Published
- 2021
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12. Quality of life of patients hospitalized in the department of surgery with the trauma and orthopedic department.
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Wieszczek M, Babuśka-Roczniak M, Brodziak-Dopierała B, and Roczniak W
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- Humans, Male, Poland, Surveys and Questionnaires, Hospitalization, Quality of Life psychology
- Abstract
Introduction: Quality of life is an interdisciplinary concept. It is broadly defined and understood by representatives of many sciences, including medical and humanities. This is because the quality of life can directly affect human behavior, decisions made by him and affect the life situation, including health., Aim of the Study: The aim of the study was to assess the quality of life of patients hospitalized in the Department of Trauma and Orthopedics Surgery., Material and Methods: The study included 105 people hospitalized in the Department of Surgery with the Trauma and Orthopedic Sub-Unit of the Healthcare Complex in Strzyżów. The diagnostic survey method was used. The research techniques that were used in the work are surveys., Results: Both, the quality of life and the level of satisfaction with health, were assessed by the respondents as good. Quality of life in the physical field is ranked the lowest. It has been noticed that the quality of life of patients decreases with age. Pain sensation diminishes the quality of life in the physical domain. On the other hand, patients who are in a relationship have a much higher assessment of the quality of life in the psychological field. Education and age do not affect the quality of life and health satisfaction., Colnclusions: Most of the patients assessed their quality of life as good or very good (83.8% of all respondents). Among the examined areas of quality of life, the respondents rated the physical domain as the lowest and the social domain the highest., (© National Institute of Public Health NIH – National Research Institute.)
- Published
- 2021
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13. Impact of Time to Initiation of Treatment on the Quality of Life of Women with Breast Cancer.
- Author
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Konieczny M, Cipora E, Roczniak W, Babuśka-Roczniak M, and Wojtaszek M
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- Aged, Female, Humans, Middle Aged, Poland epidemiology, Surveys and Questionnaires, Breast Neoplasms epidemiology, Breast Neoplasms therapy, Quality of Life, Time-to-Treatment statistics & numerical data
- Abstract
Introduction: Breast cancer is the most common malignancy in women. Due to the large number of women living with breast cancer and the increasing incidence of this cancer, it is very important to understand the factors determining the quality of life (QOL) of patients., The Aim of the Study: The aim of the study was to determine the impact of time to initiation of treatment on the quality of life of women with breast cancer., Materials and Methods: The study involved 324 women with breast cancer, treated at the Podkarpackie Oncology Centre in Brzozów, Poland. The study was conducted using a diagnostic survey, using a standardised questionnaire to measure the quality of life of women treated for breast cancer, i.e., the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC) QLQ-C30 and the QLQ-BR23 module, as well as a proprietary survey questionnaire. Statistical analysis was performed using the Statistica 10.0 software (StatSoft Inc., 2011). A p value of <0.05 was considered statistically significant., Results: The examined women had a reduced overall quality of life and health (M = 53.88). The quality of life was higher in women who consulted a doctor the earliest after noticing initial symptoms of the disease, i.e., up to one week (M = 57.58), compared to patients who delayed the decision (over four weeks; M = 47.8) ( p = 0.002). The quality of life was also considered higher by women who received treatment within two weeks of diagnosis (M = 56.79) and was lower for patients who waited for treatment for more than two months (M = 43.68). Statistically significant relationships were demonstrated for functional scales and disease intensity., Conclusions: Women diagnosed with breast cancer had a considerably lower overall quality of life. A relatively higher quality of life was experienced by patients who consulted a doctor the earliest after discovering symptoms of the disease and those whose waiting time for treatment was shorter. In a systematic manner, the individual stages of diagnosis should be maximally reduced and breast cancer treatment initiated without delay.
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- 2020
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14. Concentration of Selected Elements in the Tissues of the Knee Joint.
- Author
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Babuśka-Roczniak M, Brodziak-Dopierała B, Mitko K, Cipora E, and Roczniak W
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- Aged, Biological Monitoring, Female, Humans, Male, Middle Aged, Femur chemistry, Knee Joint chemistry, Meniscus chemistry, Metals analysis, Tibia chemistry
- Published
- 2020
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15. Serum interleukin 15 in anorexia nervosa: Comparison to normal weight and obese girls.
- Author
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Roczniak W, Mikołajczak-Będkowska A, Świętochowska E, Ostrowska Z, Ziora K, Balcerowicz S, Górska-Flak K, Milan M, and Oświęcimska J
- Subjects
- Adolescent, Body Mass Index, Female, Humans, Insulin, Interleukin-15 blood, Poland, Anorexia Nervosa blood, Insulin Resistance, Obesity blood
- Abstract
Background: Interleukin 15 (IL-15) plays a key role in the muscle-fat interaction, reducing adipose tissue mass without changes in the lean body mass and reduction of food intake. Here we assess serum IL-15 levels in girls with anorexia nervosa (AN) relative to obese and normal weight female adolescents. Methods: Serum IL-15 concentrations were evaluated using a commercially available ELISA kit in 32 Polish girls with restrictive AN, 29 girls with obesity (O), and 21 healthy controls (C). Anthropometric measurements (weight, height, BMI) and laboratory assays (serum fasting glucose, insulin, HOMA-IR, total cholesterol, HDL, LDL, triglycerides, and C-reactive protein (CRP)) were performed. Results: Mean serum IL-15 in the AN group was significantly higher than in C, but lower than in O. In all examined girls, significant positive correlations between IL-15 and body weight, BMI, insulin, HOMA, LDL, triglycerides and CRP were noted. We also observed an inverse relationship between IL-15 and HDL. Conclusions: Our study demonstrated that serum IL-15 concentrations in adolescent girls with AN and obesity are significantly elevated in comparison to normal weight controls. However, the role of IL-15 in the pathogenesis of AN and obesity remains still unclear.
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- 2020
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16. The role of adiponectin and leptin in the treatment of ovarian cancer patients.
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Słomian GJ, Nowak D, Buczkowska M, Głogowska-Gruszka A, Słomian SP, Roczniak W, Janyga S, and Nowak P
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- Adult, Aged, Aged, 80 and over, Bevacizumab therapeutic use, Biomarkers blood, Carboplatin therapeutic use, Female, Humans, Middle Aged, Ovarian Neoplasms blood, Ovarian Neoplasms diagnosis, Paclitaxel therapeutic use, Treatment Outcome, Adiponectin blood, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Leptin blood, Ovarian Neoplasms drug therapy
- Abstract
Introduction: Ovarian cancer is most frequently detected in the advanced stage. Although its pathogenesis is not fully elucidated, it is assumed that body susceptibility and hormonal disorders are responsible. The role of some cytokines as predictors in the treatment process is still investigated. The aim of the study was to determine the relationship of adiponectin and leptin with the disease severity and response to chemotherapy., Material and Methods: Forty-three ovarian cancer patients were treated by systemic treatment. Patients received 5-7 cycles of chemotherapy - paclitaxel/carboplatin with or without bevacizumab. Using standard ELISA kits before and after chemotherapy, adiponectin and leptin concentrations were determined in the blood serum., Results: The average adiponectin concentration before chemotherapy was found to be 8.83 ± 3.19 μg/ml, as compared to 10.37 ± 4.18 μg/ml (increase by 17.44%, p < 0.001) after treatment. Mean pre-treatment leptin concentration was 16.89 ± 15.54 ng/ml, and 21.77 ± 14.69 ng/ml after chemotherapy (increase by 28.89%, p < 0.01). A positive correlation was found between leptin concentration and age and BMI. There was no relationship of the disease severity with the response to treatment and the concentration of the adipokines. The leptin/adiponectin ratio (L/A) before treatment correlated with better response to chemotherapy., Conclusions: Adiponectin and leptin did not correlate with the stage of ovarian cancer and response to chemotherapy. The L/A ratio may be considered a predictor of clinical response to treatment.
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- 2019
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17. Surgical method of treatment and level of satisfaction with life among women diagnosed with breast cancer, according to time elapsed since performance of surgery.
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Cipora E, Konieczny M, Karwat ID, Roczniak W, and Babuśka-Roczniak M
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- Adult, Aged, Breast Neoplasms diagnosis, Female, Humans, Mastectomy, Mastectomy, Segmental, Middle Aged, Poland, Quality of Life, Breast Neoplasms psychology, Breast Neoplasms surgery, Patient Satisfaction
- Abstract
Introduction: In women diagnosed with breast cancer one of the methods most commonly applied in therapy is surgical treatment, which consists in a breast conserving surgery or total removal of the breast. The applied type of surgical treatment may exert an effect on the satisfaction with life of the women., Objective: The aim of the study was evaluation of the satisfaction with life among women surgically treated for breast cancer, according to the type of surgery on the breast, and the time elapsed since its performance., Material and Methods: The study included 121 patients of the Sub-Carpathian Oncology Centre in Brzozów, south-east Poland, who had undergone surgical treatment due to breast cancer. The women expressed their informed consent to participate in the study. The method of a diagnostic survey was applied., Results: The women obtained a mediocre result in satisfaction with life - 5.64 sten scores. Patients who had undergone mastectomy had a considerably lower level of satisfaction with life, compared to those after breast-conserving surgery (4.73 sten scores vs. 6.30 sten scores). Evaluations of the satisfaction with life in the examined group significantly differed according to the time elapsed since performance of surgery., Conclusions: There is a need for undertaking actions directed to women who have undergone surgical treatment for breast cancer within the period from 2-5 years, aimed at improving the satisfaction with life in this group of patients. In addition, special support should be provided for women who have undergone mastectomy, especially during the period of more than 1 and up to 2 years.
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- 2018
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18. Satisfaction with life among women with breast cancer - selected demographic and social factors.
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Cipora E, Konieczny M, Karwat ID, Roczniak W, and Babuśka-Roczniak M
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- Adult, Breast Neoplasms surgery, Demography, Female, Humans, Interpersonal Relations, Middle Aged, Personal Satisfaction, Poland, Rural Population statistics & numerical data, Surveys and Questionnaires, Breast Neoplasms psychology, Quality of Life
- Abstract
Objective: The aim of the study was evaluation of satisfaction with life among women ill with breast cancer, with consideration of selected demographic and social factors., Material and Methods: In the study participated 121 patients from the Sub-Carpathian Oncology Centre in Brzozów, Poland, who had undergone surgical treatment due to breast cancer. The method of a diagnostic survey was used. The research instruments were an author-constructed questionnaire and the Satisfaction with Life Scale., Results: Women who received surgical treatment due to breast cancer evaluated their satisfaction with life on a mediocre level. Younger patients had a lower satisfaction with life than those who were older. Women living in urban areas evaluated their satisfaction with life in more positive terms than rural women. In turn, education level, marital status and material standard had no effect on the level of satisfaction with life among the women in the study., Conclusions: According to the SWLS, women with the diagnosis of breast cancer obtained a mean result of 5.64 sten scores Younger women evaluated their satisfaction with life in more positive terms than those who were older. Women who lived in urban areas obtained a considerably higher result according to the SWLS, compared to rural inhabitants. Marital status, education level, material standard and occupational activity of the women were insignificant in the respondents' evaluation of satisfaction with life.
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- 2018
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19. Analysis of the Content of Chromium in Certain Parts of the Human Knee Joint.
- Author
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Roczniak W, Brodziak-Dopierała B, Cipora E, Jakóbik-Kolon A, Konieczny M, and Babuśka-Roczniak M
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- Age Factors, Aged, Female, Humans, Male, Middle Aged, Sex Factors, Spectrophotometry, Atomic, Chromium analysis, Knee Joint chemistry, Trace Elements analysis
- Abstract
Chromium is an essential microelement in the human body. It exerts an effect on bones by modulating their biochemical parameters: alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP). With considerable accumulation of chromium in the skeleton, the activity of alkaline phosphatase was found to decrease, which affected bone formation rate. The study objective was to analyze chromium content in the knee tissues. Tissues for analysis were obtained during endoprosthesoplasty of the knee joint and included tibia, femur, and meniscus tissues. Samples were collected from 50 patients, including 36 women and 14 men. The analysis was performed using the inductively coupled plasma atomic emission spectroscopy (ICP-AES) method, by means of a Varian 710-ES apparatus. The results revealed no significant differences in the content of chromium in the knee joint tissues between women and men. The highest level of chromium was found in the femoral bone of the knee joint, then in the meniscus, and was lowest in the tibia, although the differences were statistically insignificant. Chromium content increased with age.
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- 2018
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20. The Content of Structural and Trace Elements in the Knee Joint Tissues.
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Roczniak W, Brodziak-Dopierała B, Cipora E, Mitko K, Jakóbik-Kolon A, Konieczny M, and Babuśka-Roczniak M
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- Aged, Bone and Bones chemistry, Calcium analysis, Female, Femur chemistry, Humans, Lead analysis, Magnesium analysis, Male, Middle Aged, Phosphorus analysis, Sodium analysis, Strontium analysis, Tibia chemistry, Knee Joint chemistry, Trace Elements analysis
- Abstract
Many elements are responsible for the balance in bone tissue, including those which constitute a substantial proportion of bone mass, i.e., calcium, phosphorus and magnesium, as well as minor elements such as strontium. In addition, toxic elements acquired via occupational and environmental exposure, e.g., Pb, are included in the basic bone tissue composition. The study objective was to determine the content of strontium, lead, calcium, phosphorus, sodium and magnesium in chosen components of the knee joint, i.e., tibia, femur and meniscus. The levels of Sr, Pb, Ca, P, Na and Mg were the highest in the tibia in both men and women, whereas the lowest in the meniscus. It should be noted that the levels of these elements were by far higher in the tibia and femur as compared to the meniscus. In the components of the knee joint, the level of strontium showed the greatest variation. Significant statistical differences were found between men and women only in the content of lead.
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- 2017
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21. Correlations between iron content in knee joint tissues and chosen indices of peripheral blood morphology.
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Brodziak-Dopierała B, Roczniak W, Jakóbik-Kolon A, Kluczka J, Koczy B, Kwapuliński J, and Babuśka-Roczniak M
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- Aged, Female, Femur chemistry, Humans, Male, Meniscus chemistry, Sex Characteristics, Spectrophotometry, Atomic, Tibia chemistry, Iron analysis, Knee Joint chemistry
- Abstract
Background: Iron as a cofactor of enzymes takes part in the synthesis of the bone matrix. Severe deficiency of iron reduces the strength and mineral density of bones, whereas its excess may increase oxidative stress. In this context, it is essential to determine the iron content in knee joint tissues., Objectives: The study objective was to determine the level of iron in the tissues of the knee joint, i.e., in the femoral bone, tibia and meniscus., Material and Methods: Material for analysis was obtained during endoprosthetic surgery of the knee joint. Within the knee joint, the tibia, femur and meniscus were analyzed. Samples were collected from 50 patients, including 36 women and 14 men. The determination of iron content was performed with the ICP-AES method, using Varian 710-ES., Results: The lowest iron content was in the tibia (27.04 μg/g), then in the meniscus (38.68 μg/g) and the highest in the femur (41.93 μg/g). Statistically significant differences were noted in the content of iron in knee joint tissues., Conclusions: In patients who underwent endoprosthesoplasty of the knee joint, statistically significant differences were found in the levels of iron in various components of the knee joint. The highest iron content was found in the femoral bone of the knee joint and then in the meniscus, the lowest in the tibia. The differences in iron content in the knee joint between women and men were not statistically significant.
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- 2017
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22. Factors that Affect the Content of Cadmium, Nickel, Copper and Zinc in Tissues of the Knee Joint.
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Roczniak W, Brodziak-Dopierała B, Cipora E, Jakóbik-Kolon A, Kluczka J, and Babuśka-Roczniak M
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- Aged, Arthroplasty, Replacement, Knee, Female, Femur metabolism, Femur pathology, Femur surgery, Humans, Knee Joint pathology, Knee Joint surgery, Male, Meniscus metabolism, Meniscus pathology, Meniscus surgery, Middle Aged, Osteoarthritis, Knee pathology, Osteoarthritis, Knee surgery, Tibia metabolism, Tibia pathology, Tibia surgery, Cadmium metabolism, Copper metabolism, Knee Joint metabolism, Nickel metabolism, Osteoarthritis, Knee metabolism, Zinc metabolism
- Abstract
Osteoarthritis causes the degradation of the articular cartilage and periarticular bones. Trace elements influence the growth, development and condition of the bone tissue. Changes to the mineral composition of the bone tissue can cause degenerative changes and fractures. The aim of the research was to determine the content of cadmium (Cd), nickel (Ni), copper (Cu) and zinc (Zn) in the tibia, the femur and the meniscus in men and women who underwent a knee replacement surgery. Samples were collected from 50 patients, including 36 women and 14 men. The determination of trace elements content were performed by ICP-AES method, using Varian 710-ES. Average concentration in the tissues of the knee joint teeth amounted for cadmium 0.015, nickel 0.60, copper 0.89 and zinc 80.81 mg/kg wet weight. There were statistically significant differences in the content of cadmium, copper and zinc in different parts of the knee joint. There were no statistically significant differences in the content of cadmium, nickel, copper and zinc in women and men in the examined parts of the knee joint. Among the elements tested, copper and nickel showed a high content in the connective tissue (the meniscus) compared to the bone tissue (the tibia and the femur).
- Published
- 2017
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23. Serum FGF21 in girls with anorexia nervosa - comparison to normal weight and obese female adolescents.
- Author
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Mikolajczak A, Oswiecimska JM, Swietochowska E, Roczniak W, and Ziora KT
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- Adolescent, Blood Glucose metabolism, Body Mass Index, C-Reactive Protein metabolism, Child, Female, Humans, Insulin blood, Insulin Resistance physiology, Lipids blood, Anorexia Nervosa blood, Fibroblast Growth Factors blood, Obesity blood
- Abstract
Background: Fibroblast growth factor 21 (FGF21) is a hepatokine, myokine and adipokine of a potent influence to energy homeostasis. Data according its serum concentrations in AN are contradictory., Objectives: Analysis of serum FGF21 in girls with acute anorexia nervosa and comparison the results with normal weight and obese female adolescents considering their nutritional status, carbohydrate and lipid metabolism., Methods: Serum FGF21 concentrations were evaluated using commercially available ELISA kit in 32 Polish girls with restrictive AN (AN), 29 girls with obesity (O) and 21 healthy controls (C). Anthropometric measurements (weight, height, BMI) and laboratory assays (serum fasting glucose, insulin, HOMA-IR, total cholesterol, HDL, LDL, triglycerides, C-reactive protein) were performed., Results: Mean serum FGF-21 in the AN group was significantly lower, whereas in the O group it was significantly higher than in healthy controls. In all examined girls significant positive correlations between FGF21 and BMI were noted. We also observed significant positive relationships between serum FGF21 levels and fasting glucose, triglycerides, CRP, insulin and HOMA-IR. In all examined girls serum concentrations of this hormone correlated negatively with age and HDL-cholesterol levels., Conclusions: 1) Serum FGF21 concentrations are decreased in AN and elevated in obesity. They are independently and positively related to BMI and insulin resistance; 2) Decreased serum FGF21 in AN may support the maintenance of normal blood glucose through adjustment the insulin levels and insulin sensitivity; 3) Elevated FGF21 levels in obesity may be considered adaptive mechanism preventing insulin resistance and its metabolic consequences.
- Published
- 2017
24. Serum adiponectin levels in adolescents and young adults with growth hormone deficiency.
- Author
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Oswiecimska JM, Roczniak W, Roczniak RG, Malczyk Z, Chyra M, Mazur B, and Ziora KT
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- Adolescent, Adult, Blood Glucose metabolism, Body Composition physiology, Body Height physiology, Body Weight physiology, Dwarfism, Pituitary drug therapy, Female, Hormone Replacement Therapy, Human Growth Hormone deficiency, Human Growth Hormone therapeutic use, Humans, Insulin blood, Insulin-Like Growth Factor I metabolism, Male, Young Adult, Adiponectin blood, Adiposity physiology, Dwarfism, Pituitary blood
- Abstract
Introduction: Adiponectin (APN) is adipose tissue-derived hormone influencing energy metabolism. Growth hormone deficiency (GHD) may contribute to the development of disturbances in the hormonal function of adipose tissue (AT), and many disorders observed in untreated patients with GHD coincides with these contributed to low serum APN levels., Objectives: The assessment of serum adiponectin levels in adolescents and young adults with severe or partial GHD and analysis of relationships between serum APN and GH/IGF-1 axis function impairment as well as cardiometabolic risk factors., Design and Setting: Based on the results of insulin tolerance test (ITT) patients were qualified for one of the following groups: 1) severe GHD - SGHD (26 patients; 8 women and 18 men); 2) partial GHD - PGHD (22 patients, 7 women and 15 men); 3) normal GH status - NGHS (28 patients, 9 women and 19 men). The fourth examined group consisted of healthy individuals - H (46 participants; 15 women, 31 men). Anthropometric measurements (height, weight, BMI), analysis of body composition and serum glucose, lipids, insulin, IGF-1 and APN assays were performed in all participants., Results: There were no significant differences in the concentrations of APN between groups. After calculation of the total APN content in extracellular fluids per unit of fat tissue mass (TAPN/FM), these values were significantly lower in the SGHD (p<0.001) and correlated with the degree of impairment of the GH/IGF-1 axis functioning. In patients with GHD positive correlations between APN and serum HDL cholesterol (r=0.39, p<0.05) have been demonstrated. In the subjects with normal GH secretion serum APN correlated positively with serum HDL cholesterol (r=0.28; p<0.05), and negatively with fasting blood glucose (r=-0.31; p<0.05)., Conclusions: Severe, but not partial growth hormone deficiency impairs adiponectin production in the adipose tissue that is compensated by the increase of fat mass. The degree of GH/IGF-1 axis disruption is related to the TAPN/FM. This parameter may be potentially useful in diagnosing severe growth hormone deficiency in the adults.
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- 2017
25. New insights into the pathogenesis and treatment of irritable bowel syndrome.
- Author
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Oświęcimska J, Szymlak A, Roczniak W, Girczys-Połedniok K, and Kwiecień J
- Subjects
- Animals, Humans, Predictive Value of Tests, Risk Factors, Irritable Bowel Syndrome pathology, Irritable Bowel Syndrome therapy, Severity of Illness Index
- Abstract
Irritable bowel syndrome (IBS) is one of the most common functional gastrointestinal disorders (FGID), characterized by abdominal pain and a change in stool form that cannot be explained by structural abnormalities. Its prevalence ranges from 9 to 23% of the worldwide population. The pathophysiology of IBS is diverse and not well understood. Biopsychosocial concept assumes that the disease is a product of psychosocial factors and altered at multiple levels of gut physiology interactions. Some aetiological factors have been identified, yet. One of the most important is the disruption of brain-gut mutual communication that leads to visceral hypersensitivity. Also genetic and epigenetic factors are involved. Chronic stress may predispose to IBS as well as exacerbate its symptoms. Both quantitative and qualitative disorders of the gut microbiota are observed. There is also a relationship between the IBS symptoms and the intake of a specific type of food products. In the diarrhoea type of IBS the role of previous gastrointestinal infection is demonstrated. Recent studies have suggested that visceral hypersensitivity in patients with IBS may be secondary to the activation of the immune cells and low-grade inflammation. Clinical symptoms of IBS include abdominal pain and change in bowel habits as well as somatic and psychiatric comorbidities. IBS is diagnosed on the basis of Rome Diagnostic Criteria. Recently, their newest version (Rome IV) has been presented. The aim of this review is to summarize the past decade progress in IBS diagnosis, main pathophysiological aspects and therapeutic management strategy., (Copyright © 2016 Medical University of Bialystok. Published by Elsevier B.V. All rights reserved.)
- Published
- 2017
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26. Growth hormone deficiency in children and young adults.
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Oświęcimska J, Roczniak W, Mikołajczak A, and Szymlak A
- Abstract
Growth hormone (GH) is a naturally occurring polypeptide hormone produced by somatotropic cells in the anterior pituitary. The main function of somatotropin is stimulation of linear growth, but it also affects carbohydrate metabolism, increases bone mass and has potent lipolytic, antinatriuretic and antidiuretic effects. Growth hormone deficiency (GHD) may occur both in children and in adults. At the moment there is no gold standard for the diagnosis of GHD, and the diagnosis should take into account clinical, auxological, biochemical and radiological changes and, if necessary, genetic testing. Recent studies have highlighted that the biochemical diagnosis of GH deficiency is still imperfect. Stimuli used in the tests are non-physiological, and various substances are characterized by a different mechanism of action and potency. A few years ago it was thought that GHD treatment in children must be completed at the end of linear growth. Studies performed in the last two decades have shown that GHD deficiency in adults may result in complex clinical problems, and if untreated shortens the life expectancy and worsens its comfort. Discontinuation of GH therapy after the final height has been reached in fact negatively impacts the physiological processes associated with the transition phase, which is the period of human life between achieving the final height and 25-30 years of age. Given the adverse metabolic effects of GH treatment interruption after linear growth has been completed, the latest recommendations propose reassessment of GH secretion in the period at least one month after cessation of treatment and continuation of the therapy in case of persistent deficit.
- Published
- 2016
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27. Evaluation of the analgesic effect of morphine on models of acute nociceptive pain in rats with a central noradrenergic system lesion.
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Roczniak W, Oswiecimska JM, Brodziak-Dopierala B, Cipora E, Nowak PG, and Babuska-Roczniak M
- Subjects
- Animals, Animals, Newborn, Autonomic Nervous System Diseases chemically induced, Benzylamines, Central Nervous System Diseases chemically induced, Immersion, Male, Norepinephrine metabolism, Pain Measurement drug effects, Pain Perception drug effects, Rats, Rats, Wistar, Acute Pain drug therapy, Acute Pain etiology, Analgesics, Opioid therapeutic use, Autonomic Nervous System Diseases complications, Central Nervous System Diseases complications, Morphine therapeutic use, Nociceptive Pain drug therapy, Nociceptive Pain etiology
- Abstract
Objectives: Stimulation of some noradrenergic system receptors demonstrates a synergistic anti-nociceptive effect with the opioid system at the level of peripheral tissues, spinal cord, and supraspinal structures. Furthermore, opioids stimulate the noradrenergic descending pathways originating from the substantia nigra by presynaptic inhibition of the GABA neuron ends. It is thus important to determine whether a disruption to the adrenergic transmission obtained via DPS-4 administration to neonatal rats impacts the perception of noxious stimuli mediated by 5-HT3-serotonin receptors at the level of spinal cords or higher tiers of the central nervous system., Design & Setting: The studies were conducted with neonatal and adult rats, males of the Wistar strain in which a central noradrenergic system lesion was induced with DSP-4 on days 1 and 3 of life. Next, the evaluation of the analgesic effect of morphine was performed on 8- to 10-week-old animals using the following models of acute nociceptive pain: the hot plate test and the tail immersion test as models of acute nociceptive pain induced by a thermal stimulus, and the paw withdrawal test as a model of nociceptive pain caused by a mechanical stimulus., Results: Morphine was found to produce a longer-lasting analgesic effect in the tail immersion test in the control group than in rats. Similarly, in the paw withdrawal test, this substance generated a strong analgesic effect (with over 200% of analgesia) in the control group, whereas its action in the rats with DSP-4 lesions was statistically significant. Morphine induced analgesia at about 13-14% in the control rats when examined with the hot plate test., Conclusions: The disruption to the central noradrenergic system in an early stage of development resulted in a reduction of the analgesic effect of morphine in the models of acute pain in which the mechanisms of supraspinal perception are involved.
- Published
- 2016
28. The effect of central noradrenergic system lesion on dopamine (DA) and serotonin (5-HT) synthesis rate following administration of 5-HT3 receptor ligands in chosen parts of the rat brain.
- Author
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Roczniak W, Babuśka-Roczniak M, Kwapuliński J, Brodziak-Dopierała B, Widuchowski W, Cipora E, Nowak P, and Oświęcimska JM
- Subjects
- Analgesics, Opioid pharmacology, Animals, Autonomic Nervous System Diseases chemically induced, Benzylamines, Biguanides pharmacology, Cerebellum drug effects, Cerebellum metabolism, Hippocampus drug effects, Hippocampus metabolism, Levodopa metabolism, Ligands, Male, Morphine pharmacology, Neurotoxins, Ondansetron pharmacology, Rats, Rats, Wistar, Autonomic Nervous System Diseases metabolism, Brain Chemistry drug effects, Dopamine biosynthesis, Serotonin biosynthesis, Serotonin 5-HT3 Receptor Agonists pharmacology, Serotonin 5-HT3 Receptor Antagonists pharmacology
- Abstract
Introduction: Since little has been known about the effect of the central noradrenergic system on the reactivity of serotonin 5-HT3 receptors, the aim of the current study was to find out whether this reactivity could be altered by chemical damage to the system in adult rats in early developmental stage., Materials and Methods: Adult male Wistar rats with central noradrenergic lesion induced by DSP-4 on day 1 and 3 of life were injected with analgesic model substance - morphine, serotoninergic 5-HT3 receptor agonist (1-phenylbiguanide, PBG), 5-HT3 receptor antagonist (ondansetron) or both compounds jointly followed by decarboxylase inhibitor of aromatic amino acids (NSD-1050). After 30 min following NSD-1050 injection, the animals were decapitated using a guillotine. Chosen cerebral structures were dissected, and the contents of 5-hydroxytryptofan (5-HTP) and l-dihydroxyphenylalanine (l-DOPA) were determined using high-pressure liquid chromatography with electrochemical detection (HPLC/ED)., Results: Neither PBG nor morphine affected l-DOPA contents in the hippocampus in control rats; however, DSP-4 lesion caused a significant decrease in the synthesis rate of DA in this structure. Hippocampal contents of 5-HTP increased after morphine or PBG administration, and central noradrenergic lesion attenuated this effect. Morphine or PBG decreased cerebellar DA synthesis rate in control rats and DSP-4 lesion did not modify it. Cerebellar levels of 5-HTP increased after morphine or PBG challenge in control rats. DSP-4 lesion intensified the effect of morphine and attenuated that of PBG. Ondansetron abolished the effects mediated by PBG. We did not observe any impact of PBG or ondansetron on DA and 5-HT synthesis in the striatum., Conclusion: Damage to the central noradrenergic system in rat newborns, through altered reactivity of central 5-HT3 receptors, results in permanent disorders in serotoninergic transmission in hippocampus and cerebellum as well as dopaminergic transmission in hippocampus, which may attenuate the activity of the descending pathways that derive from these structures., (Copyright © 2014 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.)
- Published
- 2015
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29. Quality of life in transition phase in adolescents and young adults with severe and partial growth hormone deficiency.
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Oswiecimska JM, Roczniak W, Romanowicz D, Szymlak A, Mikolajczak A, Malczyk Z, Stojewska M, and Ziora KT
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- Adolescent, Adult, Age of Onset, Female, Human Growth Hormone administration & dosage, Humans, Male, Severity of Illness Index, Young Adult, Dwarfism, Pituitary psychology, Quality of Life psychology
- Abstract
Background: Transition is a term used to describe the period of adolescence after which the final adult height during growth hormone (GH) treatment is achieved. According to re-evaluation results in insulin tolerance test (ITT) patients with severe and partial growth hormone deficiency (GHD) may be distinguished., Objectives: The aim of the study was to assess QoL in patients with different degrees of GHD in transition phase., Methods: QoL was evaluated in 76 subjects aged 16-25 years with severe (SGHD, n=26), partial GHD (PGHD, n=22) and normal GH secretion (NGH, n=28) using SF-36 v.2™ Health Survey and the Quality of Life Assessment of Growth Hormone Deficiency in Adults (QoL-AGHDA) questionnaires., Results: Physical Component Score (PCS), Physical Functioning (PF) and General Health (GH) results were significantly lower in patients with SGHD than in NGH group. SF-36 v.2™ Health Survey scores in PGHD were similar as in NGH patients. There were no statistically significant differences in QoL-AGHDA scores between the examined groups. We found positive correlations between peak GH in ITT and PF (r=0.29; p=0.02) or Role Emotional (r=0.37; p=0.002) scores., Conclusions: We demonstrated that the QoL in adolescents and young adults with severe GHD in transition period is disturbed mainly in terms of physical health and emotions. These changes were detected only by generic SF-36, but not by disease-specific QoL-AGHDA questionnaire. Therefore AGHDA-QoL assessment may not be applicable in GHD patients in transition period. QoL in the patients with partial GHD is unchanged in comparison to growth hormone sufficient subjects.
- Published
- 2014
30. Influence of central noradrenergic system lesion on the serotoninergic 5-HT3 receptor mediated analgesia in rats.
- Author
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Roczniak W, Wróbel J, Dolczak L, and Nowak P
- Subjects
- Animals, Benzylamines pharmacology, Male, Morphine pharmacology, Rats, Rats, Wistar, Analgesia, Brain physiology, Norepinephrine physiology, Receptors, Serotonin, 5-HT3 physiology
- Abstract
Background: Monoaminergic pathways, impinging an adrenergic and 5-HT3 serotonin receptors, modulate nociceptive transmission, but their mechanisms and interactions has not been clarified yet., Objectives: The study was designed to investigate the influence of the neonatal noradrenergic system lesion on the antinociceptive effect of 5-HT3 receptor ligands assessed in adult animals., Material and Methods: Intact male rats were contrasted with rats in which noradrenergic nerve terminals were largely destroyed shortly after birth with neurotoxin DSP-4 [(N-(-2-chloroethyl)-N-ethyl-2-bromobenzylamine; 50 mg/kg × 2 subcutaneously (sc)], on the 1st and 3rd days of postnatal life. Control animals were injected with saline (1.0 mL/kg sc). When the rats attained 10 weeks of age, painful reactions were assessed by means of writhing and formalin tests after intraperitoneal (ip) administration of 1-phenylbiguanid (FBG; 7.5 mg/kg) or ondansetrone (1.0 mg/kg) with FBG (7.5 mg/kg). Morphine was used as a model analgesic drug., Results: Injections of morphine (7.5 mg/kg sc) evoked similar antinociception in the visceral pain model (writhing test) in both tested groups (control and DSP-4). In control rats, a 5-HT₃ receptor agonist FBG (7.5 mg/kg) elicited analgesia similar to that of morphine but the effect was significantly lower in DSP-4 treated animals. A 5-HT₃ receptor antagonist ondansetrone (1.0 mg/kg) injected before FBG did not modify the effect in the control but suppressed it in the DSP-4 group. In the formalin test, morphine produced higher analgesia in control rats in comparison with the DSP-4 group (pain intensity score of 1 point vs. 2-3 points, respectively). Ondansetrone injected before FBG alleviated the observed effect., Conclusions: Based on the obtained results, we concluded that the neonatal DSP-4 treatment alters the antinociceptive effects of morphine and serotoninergic 5-HT₃ receptor ligands. The above may explain altered (diminished) reactions of analgesics applied to patients with noradrenergic system dysfunction (e.g. depression and/or anxiety disorders).
- Published
- 2013
31. Assessment of dopamine (DA) synthesis rate in selected parts of the rat brain with central noradrenergic lesion after administration of 5-HT3 receptor ligands.
- Author
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Roczniak W
- Subjects
- Animals, Benzylamines, Brain Injuries chemically induced, Brain Injuries metabolism, Brain Stem chemistry, Brain Stem drug effects, Hypothalamus chemistry, Hypothalamus metabolism, Male, Morphine pharmacology, Ondansetron pharmacology, Rats, Rats, Wistar, Receptors, Serotonin, 5-HT3 drug effects, Receptors, Serotonin, 5-HT3 metabolism, Thalamus chemistry, Thalamus metabolism, Adrenergic Neurons drug effects, Adrenergic Neurons metabolism, Carboxy-Lyases antagonists & inhibitors, Dopamine biosynthesis, Frontal Lobe chemistry, Frontal Lobe metabolism, Levodopa analysis
- Abstract
Introduction: The study objective was to determine the effect of central noradrenergic system lesions performed in the early extrafetal life period on dopamine synthesis in the rat brain. The content of L-dihydroxyphenylalanine (L-DOPA) was assessed in the frontal lobe, thalamus, hypothalamus and brain stem of rats by high-pressure chromatography with electrochemical detection (HPLC/ED) after administration of 5-HT3 receptor ligands., Material and Methods: Adult male Wistar rats which underwent central noradrenergic lesions by DSP-4 administration (50 mg/kg m.c. i.p.) on day 1 and 3 of life received i.p. injections of the aromatic amino acid decarboxylase inhibitor (NSD-1050) in a dose of 100 mg/kg b.w. Next, 30 min after NSD-1050 injection, the animals were decapitated by guillotine. Selected brain structures were dissected and L-DOPA content was determined by HPLC/ED., Results and Conclusions: A statistically significant reduction was found in DA synthesis in the group of animals with DSP-4 lesions induced by PBG (1-phenylbiguanide, 7.5 mg/kg b.w. i.p.) and ondansetron (1.0 mg/kg b.w. i.p.). Morphine and PBG had no major effect on DA synthesis in the cerebral cortex of both control animals and in rats with noradrenergic lesions. The assessment of the effect of DSP-4 lesions on L-DOPA content in the brain stem after administration of morphine (7.5 mg/kg b.w. s.c.), PBG (7.5 mg/kg b.w. i.p.) or ondansetron (1.0 mg/kg b.w. i.p.) separately or jointly showed a statistically significant increase in the synthesis of DA in animals with DSP-4 lesions, as compared to the control group exposed to 0.9% NaCl and morphine. The analysis of the effect of DSP-4 lesions on L-DOPA content in the thalamus and hypothalamus revealed no statistically significant differences between the control groups of rats and those with DSP-4 lesions. As shown by this model, permanent noradrenergic lesions in animals in the early extra-fetal period result in increased reactivity of the central dopamine system.
- Published
- 2013
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32. Androgens concentrations and second-to fourth-digit ratio (2D:4D) in girls with congenital adrenal hyperplasia (21-hydroxylase deficiency).
- Author
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Oswiecimska JM, Ksiazek A, Sygulla K, Pys-Spychala M, Roczniak GR, Roczniak W, Stojewska M, and Ziora K
- Subjects
- 17-alpha-Hydroxyprogesterone blood, Adolescent, Adrenal Hyperplasia, Congenital drug therapy, Androstenedione blood, Anti-Inflammatory Agents therapeutic use, Child, Child, Preschool, Dehydroepiandrosterone blood, Female, Fingers growth & development, Fingers physiology, Humans, Hydrocortisone therapeutic use, Pregnancy, Prenatal Exposure Delayed Effects metabolism, Prenatal Exposure Delayed Effects pathology, Prenatal Exposure Delayed Effects physiopathology, Retrospective Studies, Testosterone blood, Virilism metabolism, Virilism pathology, Virilism physiopathology, Young Adult, Adrenal Hyperplasia, Congenital metabolism, Adrenal Hyperplasia, Congenital pathology, Androgens blood, Fingers anatomy & histology
- Abstract
Objectives: Excessive hyperandrogenism, though proper hydrocortisone supplementation is a frequent clinical problem in girls with congenital adrenal hyperplasia (CAH). This may result from autonomic regulation of androgen production established in prenatal life. It has been suggested that the length of the second finger relative to the length of the fourth finger (2D;4D ratio) is negatively related to prenatal testosterone concentration., Design and Setting: The retrospective study aimed to establish the relationship between the level of androgenization in utero determined using 2D:4D ratio and serum androgen concentrations in treated girls with CAH (21-OH deficiency) has been performed on 19 girls with CAH (21-OH deficiency) at the age of 3.7-19 years (mean 13.8 ± 4.07 years). All subjects were adequately treated with hydrocortisone (10-19 mg/m2; mean 13.81 ± 4.07 mg/m2). Anthropometric measurements of digits length were performed in all girls on X-rays obtained for bone age estimation. Apart from it, serum androgens concentrations (testosterone, androstenedione, s-DHEA) and 17-OH-progesterone (17-OHP) were assayed., Results: Mean androgens serum concentrations in examined group were: testosterone 150.21 ± 155.44 ng/ml; androstenedione 4.15 ± 5.32 ng/ml, s-DHEA 70.39 ± 85.52 µg/dl. Mean 2D:4D ratio was 0.96 ± 0.04. Analysis of correlation showed positive linear correlations between testosterone, s-DHEA and 2D:4D ratio (r=0.53, p=0.023 and r=0.53; p=0.019, respectively)., Conclusions: 2D:4D ratio parameter may be a simple test in indentification of female CAH patients prone to excessive androgen secretion despite proper treatment. The autonomization of adrenal androgens production in foetal life may cause its elevated levels in female patients with CAH although treated adequately.
- Published
- 2012
33. Spirometric parameters in malnourished girls with anorexia nervosa.
- Author
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Ziora K, Ziora D, Oswiecimska J, Roczniak W, Machura E, Dworniczak S, Tomalak W, and Dyduch A
- Subjects
- Adolescent, Body Height physiology, Body Mass Index, Body Weight physiology, Child, Female, Forced Expiratory Flow Rates, Humans, Respiratory Function Tests, Vital Capacity, Anorexia Nervosa physiopathology, Lung physiopathology, Malnutrition physiopathology
- Abstract
Repercussions of obesity on the lung function have been widely studied. The effect of serious malnutrition is less well known. The aim of study was to determine spirometric parameters in 102 malnourished girls with anorexia nervosa. Among these patients, only 71 aged 12-18 years (mean 15.6), mean BMI 15.8 kg/m(2), met the ATS/ERS forced expiratory maneuver criteria for spirometry. The most frequently observed abnormalities were: decreased IC seen in 33 (46%) girls and decreased PEF in 45 (63%) patients. Maximum voluntary ventilation was within the normal range in all but 2 subjects. Diminished values of FEV(1), FVC, FEV(1)/FVC, MEF(50) were observed in 10 (14%), 13 (18%), 3 (4%), and 3 (4%) patients, respectively. We found strong positive correlations between weight and absolute values of the examined parameters. We assume that spirometric abnormalities in anorexia are probably a result of respiratory muscle weakness and body mass loss.
- Published
- 2008
34. Histamine H(3) receptor ligands modulate L-dopa-evoked behavioral responses and L-dopa derived extracellular dopamine in dopamine-denervated rat striatum.
- Author
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Nowak P, Bortel A, Dabrowska J, Biedka I, Slomian G, Roczniak W, Kostrzewa RM, and Brus R
- Subjects
- 3,4-Dihydroxyphenylacetic Acid metabolism, Adrenergic Agents toxicity, Animals, Animals, Newborn metabolism, Dopamine Agents pharmacology, Female, Histamine H3 Antagonists pharmacology, Homovanillic Acid metabolism, Levodopa pharmacology, Microdialysis methods, Motor Activity drug effects, Oxidopamine toxicity, Piperidines pharmacology, Pregnancy, Psychomotor Performance drug effects, Rats, Rats, Wistar, Stereotyped Behavior drug effects, Thiourea pharmacology, Behavior, Animal drug effects, Corpus Striatum drug effects, Dopamine metabolism, Histamine Agonists pharmacology, Imidazoles pharmacology, Thiourea analogs & derivatives
- Abstract
To explore a recently established association between histaminergic and dopaminergic neuronal phenotypic systems in brain, we determined the effect of the respective histaminergic H(3) receptor agonist and antagonist/inverse agonist, imetit and thioperamide, on L-DOPA - derived tissue and extracellular DA and metabolite levels in the striatum of 6-hydroxydopamine (6-OHDA) - lesioned rats (i.e., parkinsonian rats). We also examined the influence of histamine H(3) ligands on L-DOPA evoked behavioral responses (locomotor activity, number of rearings, stereotyped behavior and motor coordination). Using HPLC/ED and in vivo microdialysis technique imetit (5 mg/kg, i.p.) but not thioperamide (5 mg/kg, i.p.) was shown to attenuate an L-DOPA-evoked (15 mg/kg, i.p.; carbidopa, 30 min pretreatment) increase in extracellular DA in the neostriatum of 6-OHDA-lesioned rats. However, both imetit and thioperamide increased microdialysate levels of DOPAC and HVA, probably by enhancing intraneuronal DA utilization. As indicated by neurochemical analysis of the striatum imetit produced a decrease in tissue DA content. These findings support the hypothesis that central H(3) histaminergic receptors have a modulatory role in the storage, metabolism and release of DA derived from exogenous L-DOPA challenge. Furthermore, evidence from behavioral studies indicate that histamine H3 receptor blockade markedly improved motor coordination. Conversely, histamine H(3) receptor stimulation, being without effect on motor coordination, enhanced vertical activity in rats. From the above we conclude that the histamine H(3) agonism may augment motor dyskinesia in Parkinson's disease (PD) patients and presumably worsen L-DOPA therapy. Consequently, the histaminergic system represents a viable target for modulating the effectiveness of L-DOPA therapy in Parkinson's disease.
- Published
- 2008
- Full Text
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35. Effects of neuroendocrine changes on results of ambulatory blood pressure monitoring (ABPM) in adolescent girls with anorexia nervosa.
- Author
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Oświecimska J, Ziora K, Adamczyk P, Roczniak W, Pikiewicz-Koch A, Stojewska M, and Dyduch A
- Subjects
- Adolescent, Anorexia Nervosa complications, Blood Pressure Monitoring, Ambulatory, Bradycardia etiology, Bradycardia physiopathology, Case-Control Studies, Child, Circadian Rhythm physiology, Estradiol blood, Female, Follicle Stimulating Hormone blood, Heart Rate physiology, Humans, Hydrocortisone blood, Luteinizing Hormone blood, Adolescent Development physiology, Anorexia Nervosa physiopathology, Blood Pressure physiology, Neurosecretory Systems physiology
- Abstract
Objectives: Anorexia nervosa (AN) is characterized by marked neuroendocrine and autonomic dysfunctions. In the recent studies using automatic blood pressure monitoring (ABPM), lower BP values and lack of circardian variation of BP in anorectic patients were demonstrated. Unfortunately effects of hormonal changes, that may explain BP abnormalities were not analysed together., Design: The aim of our study was the assessment of ABPM and hormonal status in anorectic girls., Settings: The study was performed on hospitalized 25 female anorectic adolescents aged 12-18 years. Control group was 17 age and height matched girls with normal weight and negative history for hypertension. ABPM was performed between 5 and 7 day of hospitalization, every 30 minutes during active period and every 60 minutes during sleep. Hormones (FSH, LH, estradiol, cortisol and fT4) serum concentrations were also evaluated., Results: Mean systolic BP values were significantly lower in patients with AN in comparison to controls. Maximal diastolic and mean arterial pressure values for the whole day and active period but not for sleep were lower in AN than in controls. Anorectic girls showed tendency to night-time bradycardia. Moreover, there were no physiological circadian variations of BP in AN., Conclusions: We conclude that hormonal regulation of blood pressure and heart rate in anorectic patients is at least partially preserved. Lower blood pressure values, bradycardia and lack of physiological night fall of BP in anorectic patients may result from altered autonomic system function resulting from hormonal disturbances and other centrally mediated mechanisms.
- Published
- 2007
36. Toxic influence of subchronic paraquat administration on dopaminergic neurons in rats.
- Author
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Kuter K, Smiałowska M, Wierońska J, Zieba B, Wardas J, Pietraszek M, Nowak P, Biedka I, Roczniak W, Konieczny J, Wolfarth S, and Ossowska K
- Subjects
- Animals, Caudate Nucleus drug effects, Caudate Nucleus metabolism, Caudate Nucleus pathology, Drug Administration Schedule, Gene Expression Regulation, Enzymologic drug effects, Glutamate Decarboxylase genetics, Herbicides administration & dosage, Herbicides toxicity, Isoenzymes genetics, Nerve Degeneration pathology, Neurons pathology, Paraquat administration & dosage, Parkinsonian Disorders chemically induced, Putamen drug effects, Putamen metabolism, Putamen pathology, RNA, Messenger genetics, Rats, Dopamine physiology, Neurons drug effects, Paraquat toxicity
- Abstract
Paraquat is a toxin suggested to contribute to pathogenesis of Parkinson's disease. The aim of the present study was to examine toxic influence of subchronic treatment with this pesticide (5 days, one injection per day, 2-3 days of withdrawal) on dopaminergic, serotonergic, noradrenergic and GABAergic neurons. Paraquat decreased the number of tyrosine hydroxylase-immunoreactive (TH-ir) neurons in the substantia nigra by 22% (measured 3 days after withdrawal). Two days after withdrawal the levels of the dopamine metabolites and dopamine turnover in the caudate-putamen, substantia nigra and prefrontal cortex were reduced by ca. 20-60%, and the binding of [(3)H]GBR 12,935 to dopamine transporter dropped by 25-40% in the caudate-putamen. Three days after paraquat withdrawal, the level of dopamine in the caudate-putamen was significantly increased, and earlier decreases in DOPAC and HVA in the substantia nigra, as well as [(3)H]GBR 12,935 binding in the caudate-putamen were reversed. Moreover, an increase in serotonin turnover in the caudate-putamen and prefrontal cortex, and noradrenaline level in the former structure was observed 2-3 days after paraquat withdrawal. Three days after the last paraquat injection 24-35% decreases in the proenkephalin mRNA levels and 5-7% reduction in glutamic acid decarboxylase (GAD)67 mRNA were found in the caudate-putamen. The present study suggests that subchronic paraquat administration triggers processes characteristic of early stages of dopaminergic neuron degeneration, and activates compensatory mechanisms involving dopaminergic, noradrenergic, serotonergic and GABAergic transmissions.
- Published
- 2007
- Full Text
- View/download PDF
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