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7. Predictive factors of early pregnancy loss during in vitro fertilization/ intracytoplasmic sperm injection (IVF/ICSI): retrospective study on 1806 embryo transfers

Author

Morcel, Karine, primary, Merviel, Philippe, additional, James, Pandora, additional, Bouée, Sarah, additional, Guillou, Mathilde Le, additional, Pertuisel, Diane, additional, Chabaud, Jean-Jacques, additional, Roche, Sylvie, additional, Perrin, Aurore, additional, Drapier, Hortense, additional, and Beauvillard, Damien, additional

Published
2023
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8. Live Birth Rates in Women Under 38 Years Old with AMH Level < 1.2 ng/ml in the First In Vitro Fertilization + / − Intracytoplasmic Sperm Injection: Retrospective Study and Arguments for Care

Author

Morcel, Karine, primary, Merviel, Philippe, additional, Pertuisel, Diane, additional, James, Pandora, additional, Bouée, Sarah, additional, Le Guillou, Mathilde, additional, Chabaud, Jean-Jacques, additional, Roche, Sylvie, additional, Drapier, Hortense, additional, Perrin, Aurore, additional, and Beauvillard, Damien, additional

Published
2022
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9. IRIGAM - Identification de résistances à l’infection des grains par Fusariumgraminearum et à l’accumulation des mycotoxines au sein des variétés de bléfrançaises grâce à la mise en place de nouvelles technologies de phénotypage

Author

Lasserre - Zuber, Pauline, Chavée, Marianne, Faure, Marlène, Roche, Sylvie, Soudière, Olivier, Mutterer, Jerome, Maignel, Jean-Philippe, Pousset, Emilien, Lem, Patricia, Leremboure, Martin, Devaux, Pierre, Serre, Frédérique, Cadot, Valerie, Saintenac, Cyrille, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Groupe d'Etude et de contrôle des Variétés Et des Semences (GEVES), Unité Expérimentale PHénotypage Au Champ des Céréales (UE1375) (PHACC), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), BioGEVES, Institut National de la Recherche Agronomique (INRA)-Ministère en charge de l'Agriculture-Groupement National Interprofessionnel des Semences, Institut de Chimie de Clermont-Ferrand (ICCF), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Florimond Desprez, and CASDAR semences et sélection végétaleONEMA

Subjects
Fusarium head blight, Blé tendre, Phenotyping, [SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Fusariose de l’épi, Phénotypage, Breadwhea, Deoxynivalenol
Abstract

International audience; Kernels contamination by mycotoxins and especially deoxynivaneol (DON) represents a major sanitarythreat for the cereals. Here, we compared a set of different methodologies to evaluate Fusarium damagedkernels and DON contaminated flours. This will allow to routinely assessing these traits in breedingprograms. Thanks to these methodologies, we identified seven wheat genomic regions involved inresistance to DON accumulation. Molecular markers associated with these regions are now available tobreed for varieties with improved resistance, a high priority of the grain sector.; La contamination des grains par les mycotoxines et notamment le deoxynivalenol (DON) est un problèmesanitaire majeur chez les céréales. Cette étude a permis de comparer un ensemble exhaustif deméthodologies pour évaluer le taux de grains symptomatiques suite à une attaque de Fusariumgraminearum et la contamination des farines par le DON pour permettre une évaluation en routine de cescaractères dans les programmes de sélection. Grace à ces nouvelles méthodologies, le projet aégalement mis en avant sept régions du génome de blé impliquées dans la résistance aux DON ainsi quedes marqueurs moléculaires utilisables en sélection pour construire des variétés plus résistantes, unenjeu prioritaire pour la filière céréalière

Published
2021
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13. Can Ratios Between Prognostic Factors Predict the Clinical Pregnancy Rate in an IVF/ICSI Program with a GnRH Agonist-FSH/hMG Protocol? An Assessment of 2421 Embryo Transfers, and a Review of the Literature

Author

Merviel, Philippe, primary, Menard, Michel, additional, Cabry, Rosalie, additional, Scheffler, Florence, additional, Lourdel, Emmanuelle, additional, Le Martelot, Marie-Thérèse, additional, Roche, Sylvie, additional, Chabaud, Jean-Jacques, additional, Copin, Henri, additional, Drapier, Hortense, additional, Benkhalifa, Moncef, additional, and Beauvillard, Damien, additional

Published
2020
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14. Progesterone levels on the human chorionic gonadotropin trigger day affect the pregnancy rates for embryos transferred at different stages of development in both general and selected IVF/ICSI populations

Author

Merviel, Philippe, primary, Bouée, Sarah, additional, Jacamon, Anne-Solenn, additional, Chabaud, Jean-Jacques, additional, Martelot, Marie-Thérèse Le, additional, Roche, Sylvie, additional, Rince, Camille, additional, Drapier, Hortense, additional, Perrin, Aurore, additional, and Beauvillard, Damien, additional

Published
2020
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19. Polyphasic characterization and genetic relatedness of low-virulence and virulent Listeria monocytogenes isolates

Author

Roche Sylvie M, Grépinet Olivier, Kerouanton Annaëlle, Ragon Marie, Leclercq Alexandre, Témoin Stéphanie, Schaeffer Brigitte, Skorski Gilbert, Mereghetti Laurent, Le Monnier Alban, and Velge Philippe

Subjects
Microbiology, QR1-502
Abstract

Abstract Background Currently, food regulatory authorities consider all Listeria monocytogenes isolates as equally virulent. However, an increasing number of studies demonstrate extensive variations in virulence and pathogenicity of L. monocytogenes strains. Up to now, there is no comprehensive overview of the population genetic structure of L. monocytogenes taking into account virulence level. We have previously demonstrated that different low-virulence strains exhibit the same mutations in virulence genes suggesting that they could have common evolutionary pathways. New low-virulence strains were identified and assigned to phenotypic and genotypic Groups using cluster analysis. Pulsed-field gel electrophoresis, virulence gene sequencing and multi-locus sequence typing analyses were performed to study the genetic relatedness and the population structure between the studied low-virulence isolates and virulent strains. Results These methods showed that low-virulence strains are widely distributed in the two major lineages, but some are also clustered according to their genetic mutations. These analyses showed that low-virulence strains initially grouped according to their lineage, then to their serotypes and after which, they lost their virulence suggesting a relatively recent emergence. Conclusions Loss of virulence in lineage II strains was related to point mutation in a few virulence genes (prfA, inlA, inlB, plcA). These strains thus form a tightly clustered, monophyletic group with limited diversity. In contrast, low-virulence strains of lineage I were more dispersed among the virulence strains and the origin of their loss of virulence has not been identified yet, even if some strains exhibited different mutations in prfA or inlA.

Published
2012
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23. Les sérotypes de Salmonella induisent différentes interactions cellulaires et moléculaires avec les cellules épithéliales intestinales porcines IPEC-1

Author

Berri, Mustapha, Olivier, Michel, Chevaleyre, Claire, Pereira Melo, Sandrine, Roche, Sylvie, Velge, Philippe, Payant, Isabelle, Meurens, François, Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Institut National de la Recherche Agronomique (INRA), Ecole Nationale Vétérinaire Agroalimentaire et de l'Alimentation Nantes Atlantique (ONIRIS), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut du Porc (IFIP). FRA. Institut National de la Recherche Agronomique (INRA), FRA., and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
culture cellulaire, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, interaction cellulaire, réponse immunitaire, RT-qPCR, salmonella, microbial culture, cellule épithéliale intestinale, swine, immunologic reactions, porc
Abstract

Session : Santé animale; The ability of Salmonella serotypes to infect orally a given host and induce pathology relies on their cellular and molecular interactions with the intestinal epithelium. In pigs, the serotype S. Choleraesuis induces septicemia while S. Typhimurium induces gastroenteritis or an asymptomatic carrier state of the bacteria. S. Typhisuis, a serovar rarely studied, is able to cause hemorrhagic diarrhea and colon inflammation. An in vitro approach was used to establish a correlation between the capacity of different Salmonella serotypes to adhere, invade and induce an immune response depending on the porcine intestinal epithelial cells IPEC-1. To this end, S.Choleraesuis, S. Typhimurium and S. Typhisuis were evaluated using this in vitro cellular model in comparison with S. Dublin andS.Gallinarum, two host-specific of cattle and poultry respectively. We have shown that with S. Typhimurium and S. Choleraesuis, the invasion percentage of non-polarized IPEC-1 cells was higher than that obtained with S. Typhisuis, S. Dublin or S. Gallinarum.In contrast, S. Typhisuis invaded successfully polarized IPEC-1 cells as well as S. Choleraesuis and S. Typhimurium but unlike S.Gallinarum or S. Dublin. The analysis of the immune response induction, using RT-qPCR, showed a significant increase of inflammatorycytokine expression such as IL-8, TNF alpha IL1 alpha or IL1 beta CCL20 and CCL25 chemokines and TLR4 receptor in polarized IPEC-1 cells infectedwith S. Typhimurium and S. Typhisuis serotypes. Comparatively, S. Choleraesuis infection did not induce any change in the expressionof these mediators. Thus, the first cell interaction steps with polarized IPEC-1 are able to distinguish serotypes based on their targetspecies whereas induction of the immune response would differentiate a pig’s serotypes according to their pathogenesis.

Published
2017

24. Invasion abilities of 'Salmonella' strains unable to express one or more known invasion factors

Author

Roche, Sylvie M., Wiedemann, Agnès, Rosselin, Manon, Trotereau, Jérôme, Schaeffer, Samantha, Velge, Philippe, Virlogeux-Payant, Isabelle, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, American Society for Microbiology. USA., and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

25. The T3SS-1 : not the only way for Salmonella to invade non phagocytic cells

Author

Roche, Sylvie, Wiedemann, Agnès, Rosselin, Manon, Trotereau, Jérôme, Schaeffer, Samantha, Velge, Philippe, Virlogeux-Payant, Isabelle, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

26. Challenges in ovarian stimulation

Author

Merviel, Philippe, LE Martelot, Marie, Roche, Sylvie, Lelièvre, Caroline, Ménard, Michel, Auget, Stéphanie, Beauvillard, Damien, Amice, Véronique, De Braekeleer, Marc, Groupe d'Etude de la Thrombose de Bretagne Occidentale (GETBO), Université de Brest (UBO)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Laboratoire Informatique, Image et Interaction (L3I), Université de La Rochelle (ULR), Immunologie et Pathologie (EA2216), Université de Brest (UBO)-IFR148, Génétique, génomique fonctionnelle et biotechnologies (UMR 1078) (GGB), EFS-Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Brestois Santé Agro Matière (IBSAM), Calvez, Ghislaine, Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO), Laboratoire Informatique, Image et Interaction - EA 2118 (L3I), La Rochelle Université (ULR), Institut Brestois Santé Agro Matière (IBSAM), and Université de Brest (UBO)-Université de Brest (UBO)-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
endocrine system, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Pregnancy Rate, [SDV]Life Sciences [q-bio], Oocyte Retrieval, Fertilization in Vitro, [SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics, Embryo Transfer, female genital diseases and pregnancy complications, [SDV] Life Sciences [q-bio], Gonadotropin-Releasing Hormone, [SDV.MHEP.GEO] Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics, Ovulation Induction, Pregnancy, Humans, Female, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Maternal Age
Abstract

International audience; In 2016, ovarian stimulation faces two main challenges: how to obtain good quality oocytes while not endangering the patients treated, but also limited by maternal age and poor ovarian responders (POR). The first IVF birth, Louise Brown, was obtained from a natural cycle. With the introduction, in the 1980s of gonadotropin releasing hormone agonists (GnRHa) and in the 2000s of GnRH antagonists (GnRHant), stimulation became plurifollicular (and source of consequences). Today, only about 50% of the transferred blastocysts after IVF lead to a pregnancy. The purpose of this review was to describe the current challenges and limits of ovarian stimulation.

Published
2016

27. Mesurer le niveau de résistance quantitative des variétés de tournesol face au mildiou dans les processus de sélection et d’évaluation variétale : un enjeu fort pour la gestion durable du risque

Author

Mestries, Emmannelle, Auclert, B., Poisson-Bammé, B., Penaud, Annette, Grimault, Valerie, Perrot, S., Robert, A., Roche, Sylvie, Serre, Frédéric, Tourvieille de Labrouhe, Denis, Vear, Felicity, Boniface, Marie-Claude, Bordat, Amandine, Pouilly, Nicolas, Vincourt, Patrick, Munos, Stephane, Terres Inovia, Station Nationale d’Essais de Semences (SNES), UE 1375 Phénotypage Au Champ des Céréales, Institut National de la Recherche Agronomique (INRA)-Santé des plantes et environnement (S.P.E.)-Biologie et Amélioration des Plantes (BAP)-Phénotypage Au Champ des Céréales (PHACC), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Laboratoire des interactions plantes micro-organismes (LIPM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), and Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)

Subjects
phenotyping, quantitative resistance, résistance quantitative, conditions contrôlées, phénotypage, [SDV]Life Sciences [q-bio], Plasmopara halstedii, controlled conditions
Abstract

La résistance quantitative du tournesol (Helianthus annuus) au mildiou (Plasmopara halstedii), mise en évidence récemment, est difficile à évaluer. Un réseau de onze expérimentations associant la GEVESSNES, deux laboratoires de l’INRA et Terres Inovia a été construit afin de proposer une méthodologie permettant de mieux caractériser et quantifier cette résistance. En plus de lignées témoins, 17 lignées recombinantes obtenues par l’INRA et portant entre 0 et 3 QTL (quantitative trait loci) impliqués dans la résistance quantitative du tournesol au mildiou ont été testées face à 5 pathotypes de mildiou : 710, 704, 714, 304 et 334. Bien que des différences aient été observées entre laboratoires, les résultats ont permis de poser les bases d’un futur protocole utilisable en routine pour valoriser ce type de résistance dès la création variétale jusqu’à l’inscription puis la post-inscription des variétés., A quantitative resistance of sunflower (Helianthus annuus) to downy mildew (Plasmopara halstedii) has been identified a few years ago. An experimental network involving several labs (GEVES-SNES, INRA and Terres Inovia) over several years aimed at describing and quantifying such a quantitative response. It was hoped to propose a methodology useable to improve sustainable resistance. In addition to check varieties and breeding lines, 17 recombinant inbred lines carrying 0 to 3 quantitative trait loci alleles for quantitative resistance were tested with five downy mildew pathotypes (710, 704, 714, 304, 334). While the experimental results revealed some discrepancies between experiments, this study provided a first experimental protocol to evaluate quantitative resistance both for breeding and for variety registration and development

Published
2016
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28. Epithelial cell invasion by salmonellatyphimurium induces modulation of genes controlled by aryl hydrocarbon receptor signaling and involved in extracellular matrix biogenesis

Author

Chaussé, Anne-Marie, Roche, Sylvie M., Moroldo, Marco, Hennequet-Antier, Christelle, Holbert, Sébastien, Kempf, Florent, Barilleau, Emilie, Trotereau, Jérome, and Velge, Philippe

Abstract

ABSTRACTSalmonellais the only bacterium able to enter a host cell by the two known mechanisms: trigger and zipper. The trigger mechanism relies on the injection of bacterial effectors into the host cell through the Salmonellatype III secretion system 1. In the zipper mechanism, mediated by the invasins Rck and PagN, the bacterium takes advantage of a cellular receptor for invasion. This study describes the transcriptomic reprogramming of the IEC-6 intestinal epithelial cell line to SalmonellaTyphimurium strains that invaded cells by a trigger, a zipper, or both mechanisms. Using S. Typhimurium strains invalidated for one or other entry mechanism, we have shown that IEC-6 cells could support both entries. Comparison of the gene expression profiles of exposed cells showed that irrespective of the mechanism used for entry, the transcriptomic reprogramming of the cell was nearly the same. On the other hand, when gene expression was compared between cells unexposed or exposed to the bacterium, the transcriptomic reprogramming of exposed cells was significantly different. It is particularly interesting to note the modulation of expression of numerous target genes of the aryl hydrocarbon receptor showing that this transcription factor was activated by S. Typhimurium infection. Numerous genes associated with the extracellular matrix were also modified. This was confirmed at the protein level by western-blotting showing a dramatic modification in some extracellular matrix proteins. Analysis of a selected set of modulated genes showed that the expression of the majority of these genes was modulated during the intracellular life of S. Typhimurium.

Published
2023
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29. Heterogeneity of persistence and infectivity of Salmonella Senftenberg strains

Author

Boumart, Zineb, Roche, Sylvie, Virlogeux-Payant, Isabelle, Chemaly, Marianne, Velge, Philippe, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Agence Française de Sécurité Sanitaire des Aliments (AFSSA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], pouvoir pathogène, [SDV]Life Sciences [q-bio], Salmonella Senftenberg
Abstract

National audience; In poultry production, Salmonella Senftenberg has always been associated to the hatchery environment, but has recently become more frequent in poultry farms. Furthermore, some strains were responsible of several human infections throughout the world. In order to explain the emergence of persistent clones in poultry houses, we identified strains showing different persistence phenotypes in chickens. The non-persistent strains were eliminated progressively after the first week of infection, while the persistent strains were able to colonize the caeca of chickens at high levels similar to S. Enteritidis. The assessment of the pathogenicity of these two phenotypes showed that all S. Senftenberg strains, contrary to S. Enteritidis were able to induce a strong systemic infection neither in orally infected mice and chickens nor in mice infected subcutaneously. The in vitro analysis with epithelial cells and macrophages suggests that this poor infectivity could be related to an alteration in their capacity to invade nonphagocytic cells. The comparison between persistent and non-persistent S. Senftenberg strains showed that persistent strains are more able to colonize and persist in spleen of chickens and mesenteric lymph-nodes of mice. No difference has been observed between these strains in their ability to grow in the caecal content. All together, our findings suggest that the existence of distinct persistent phenotypes within S. Senftenberg could explain the recent emergence of this serotype in poultry flocks and the higher capacity of persistent strains to colonize the animal tissues could be a possible explanation for this increased persistence.

Published
2011

31. Adaptation de L monocytogenes à ses environnements

Author

Roche, Sylvie, Velge, Philippe, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2007

32. Détection, prévalence et caractérisation des souches naturelles de Listeria monocytogenes de faible virulence

Author

Le Barzic-Roche, Sylvie, ProdInra, Migration, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Université François Rabelais (Tours), and Philippe Velge

Subjects
[SDV] Life Sciences [q-bio], INFECTIONS ANIMALES ET CELLULAIRES, LISTERIA MONOCYTOGENES, [SDV]Life Sciences [q-bio], FAIBLE VIRULENCE, PrfA, MLST
Abstract

Diplôme : Dr. d'Université

Published
2006

33. Two structures of vesicular stomatitis virus glycoprotein

Author

Bressanelli, Stéphane, Roche, Sylvie, Rey, F, Gaudin, Yves, ProdInra, Migration, Virologie moléculaire et structurale (VMS), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), and Inconnu

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2006

34. A propos d’un changement de conformation majeur mais réversible : cristallisation des formes pré- et post-fusion de la glycoprotéine du virus de la stomatite vésiculaire

Author

Bressanelli, Stéphane, Roche, Sylvie, Rey, F, Gaudin, Yves, Virologie moléculaire et structurale (VMS), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Inconnu, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2006

35. Atomic structure of VSV glycoprotein in both its neutral and low pH conformation

Author

Roche, Sylvie, Bressanelli, Stéphane, Rey, F, Lepault, Jean, Gaudin, Yves, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Virologie moléculaire et structurale (VMS), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Inconnu, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2006

36. Structures de la glycoprotéine G de VSV sous ses conformations neutres et acides

Author

Roche, Sylvie, Bressanelli, Stéphane, Rey, Fa, Lepault, Jean, Gaudin, Yves, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Virologie moléculaire et structurale (VMS), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), and Inconnu

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2006

37. Relation entre l'analyse phénotypique et génotypique de souches faiblement virulentes de Listeria monocytogènes

Author

Temoin, Stephanie, Roche, Sylvie, Gracieux, P., Albert, Isabelle, Grépinet, Olivier, Cossart, Pascale, Velge, Philippe, Unité de Pathologie Infectieuse et Immunologie [Nouzilly] (PII), Institut National de la Recherche Agronomique (INRA), Unité de recherche Mathématiques et Informatique Appliquées (MIA), Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Biotechnocentre. FRA., UR Infectiologie animale et Santé publique (UR IASP), Infectiologie Santé Publique (ISP-311), Université de Tours-Institut National de la Recherche Agronomique (INRA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Abstract

Session Posters - Poster; Actuellement, toute souche de Listeria monocytogenes est considérée comme potentiellement virulente. Pourtant, une variabilité de la virulence observée in vivo et in vitro a été décrite dans la littérature. Un test de détection in vitro de plages de lyse suivi d’un test d’inoculation souscutanée plantaire sur souris nous ont permis de caractériser 26 souches faiblement virulentes. Ces souches sont réparties, selon une classification hiérarchique ascendante, en 4 groupes distincts selon 4 variables phénotypiques : taux de pénétration intracellulaire, nombre de plages de lyse formées et dosages des activités phospholipases (PI-PLC et PC-PLC). A partir des données phénotypiques, les gènes de virulence intervenant dans les différentes étapes du cycle d’infection et pouvant être liés à cette faible virulence ont été séquencés. Sur les 11 souches du groupe I, qui ne pénètrent pas dans les cellules, ne forment pas de plage de lyse et n’expriment aucune activité phospholipasique, 8 présentent une substitution dans le régulateur transcriptionnel PrfA et 3, un PrfA tronqué. Une des 3 souches du groupe II, qui pénètrent dans les cellules, ne forment pas de plage de lyse et n’expriment que l’activité PI-PLC, présente 4 substitutions dans PC-PLC, et 4 dans ActA. De même, 5 des 6 souches du groupe III, qui pénètrent dans les cellules, ne forment pas de plage de lyse et n’expriment que l’activité PC-PLC,présentent 3 substitutions dans PI-PLC et 22 dans ActA. Les 6 souches du groupe IV qui ne colonisent que faiblement les souris, ne se différencient quasiment pas des souches virulentes par leurs caractères phénotypiques. Ces résultats suggèrent un lien entre le phénotype des ces souches et les substitutions génotypiques observées. Ces substitutions retrouvées chez plusieurs souches suggèrent une évolution commune. Nous devrons déterminer si les substitutions observées dans les différents gènes de virulence sont la cause de la faible virulence.

Published
2004

39. Validation expérimentale de la faible virulence de souches naturelles de Listeria monocytogenes

Author

Roche, Sylvie M., Gracieux, P., Albert, Isabelle, Jacquet, C., Martin, P.M.V., Velge, Philippe, Unité de Pathologie Infectieuse et Immunologie [Nouzilly] (PII), Institut National de la Recherche Agronomique (INRA), Unité de recherche Mathématiques et Informatique Appliquées (MIA), Centre National de Référence des Listeria-G5 Microorganismes et Barrières de l'Hôte (CNR-CCOMS), Institut Pasteur [Paris], Unité de pathologie infectieuse et immunologie, UE 0993 TOURS PII UE Unité de pathologie infectieuse et immunologie, and Institut Pasteur [Paris] (IP)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

absent

Published
2003

40. Pathway of virus-induced membrane fusion studied with liposomes

Author

Roche, Sylvie, Gaudin, Yves, ProdInra, Migration, Unité de Pathologie Infectieuse et Immunologie [Nouzilly] (PII), Institut National de la Recherche Agronomique (INRA), Virologie moléculaire et structurale (VMS), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), and INC INC

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2003

41. Deciphering why

Author

Rossignol, Aurore, primary, Roche, Sylvie M, additional, Virlogeux-Payant, Isabelle, additional, Wiedemann, Agnès, additional, Grépinet, Olivier, additional, Fredlund, Jennifer, additional, Trotereau, Jérôme, additional, Marchès, Olivier, additional, Quéré, Pascale, additional, Enninga, Jost, additional, and Velge, Philippe, additional

Published
2014
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42. Heterogeneity of Persistence of Salmonella enterica Serotype Senftenberg Strains Could Explain the Emergence of this Serotype in Poultry Flocks

Author

Boumart, Zineb, primary, Roche, Sylvie M., additional, Lalande, Françoise, additional, Virlogeux-Payant, Isabelle, additional, Hennequet-Antier, Christelle, additional, Menanteau, Pierrette, additional, Gabriel, Irène, additional, Weill, François-Xavier, additional, Velge, Philippe, additional, and Chemaly, Marianne, additional

Published
2012
Full Text
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43. Listeria rocourtiae sp. nov.

Author

Leclercq, Alexandre, primary, Clermont, Dominique, additional, Bizet, Chantal, additional, Grimont, Patrick A. D., additional, Le Flèche-Matéos, Anne, additional, Roche, Sylvie M., additional, Buchrieser, Carmen, additional, Cadet-Daniel, Véronique, additional, Le Monnier, Alban, additional, Lecuit, Marc, additional, and Allerberger, Franz, additional

Published
2010
Full Text
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