108 results on '"Rocha, Cristina M. R."'
Search Results
2. Solubilization and Hydrolysis of Porcine Coagulated Blood Protein Using Sub-Critical Solvent Extraction
- Author
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Marques, Bianca, Nunes, Rafaela, Araújo-Rodrigues, Helena, Pintado, Manuela, Pereira, Ricardo N., Teixeira, José A., and Rocha, Cristina M. R.
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- 2024
- Full Text
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3. Life Cycle Energy and Carbon Footprint of Native Agar Extraction from Gelidium sesquipedale Using Alternative Technologies
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Pereira, Sara G., Mata, Teresa M., Pereira, Ricardo N., Teixeira, José A., Rocha, Cristina M. R., Martins, António A., Förstner, Ulrich, Series Editor, Rulkens, Wim H., Series Editor, Caetano, Nídia S., editor, and Felgueiras, Manuel Carlos, editor
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- 2023
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4. Recent Advances in the Valorization of Algae Polysaccharides for Food and Nutraceutical Applications: a Review on the Role of Green Processing Technologies
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Gomes-Dias, Joana S., Teixeira, José A., and Rocha, Cristina M. R.
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- 2022
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5. Improving agar properties of farmed Gracilaria gracilis by using filtered sunlight
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Silva-Brito, Francisca, Pereira, Sara G., Rocha, Cristina M. R., da Costa, Elisabete, Domingues, M. Rosário, Azevedo, Andreina, Kijjoa, Anake, Abreu, Helena, and Magnoni, Leonardo
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- 2021
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6. Postharvest Quality Improvement of Tomato (Solanum lycopersicum L.) Fruit Using a Nanomultilayer Coating Containing Aloe vera
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Consejo Nacional de Humanidades, Ciencias y Tecnologías (México), Fundação para a Ciência e a Tecnologia (Portugal), European Commission, Gobierno del Estado de Coahuila, Flores-López, María L., Vieira, Jorge M., Rocha, Cristina M. R., Lagarón Cabello, José María, Cerqueira, Miguel A., Jasso de Rodríguez, Diana, Vicente, António, Consejo Nacional de Humanidades, Ciencias y Tecnologías (México), Fundação para a Ciência e a Tecnologia (Portugal), European Commission, Gobierno del Estado de Coahuila, Flores-López, María L., Vieira, Jorge M., Rocha, Cristina M. R., Lagarón Cabello, José María, Cerqueira, Miguel A., Jasso de Rodríguez, Diana, and Vicente, António
- Abstract
The effectiveness of an alginate/chitosan nanomultilayer coating without (NM) and with Aloe vera liquid fraction (NM+Av) was evaluated on the postharvest quality of tomato fruit at 20 °C and 85% relative humidity (RH) to simulate direct consumption. Both nanomultilayer coatings had comparable effects on firmness and pH values. However, the NM+Av coating significantly reduced weight loss (4.5 ± 0.2%) and molds and yeasts (3.5–4.0 log CFU g−1) compared to uncoated fruit (16.2 ± 1.2% and 8.0 ± 0.0 log CFU g−1, respectively). It notably lowered O2 consumption by 70% and a 52% decrease in CO2 production, inhibiting ethylene synthesis. Visual evaluation confirmed NM+Av’s efficacy in preserving the postharvest quality of tomato. The preservation of color, indicated by the Minolta color (a*/b*) values, demonstrated NM+Av’s ability to keep the light red stage compared to uncoated fruit. The favorable effects of NM+Av coating on enhancing postharvest quality indicates it as a potential alternative for large-scale tomato fruit preservation.nanomultilayer coatings; tomato; Aloe vera liquid fraction; gas barrier properties; postharvest quality
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- 2024
7. Postharvest Quality Improvement of Tomato (Solanum lycopersicum L.) Fruit Using a Nanomultilayer Coating Containing Aloe vera
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Flores-López, María L., primary, Vieira, Jorge M., additional, Rocha, Cristina M. R., additional, Lagarón, José M., additional, Cerqueira, Miguel A., additional, Jasso de Rodríguez, Diana, additional, and Vicente, António A., additional
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- 2023
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8. Valorization of Passion Fruit Stalk by the Preparation of Cellulose Nanofibers and Immobilization of Trypsin
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Rodriguez-Restrepo, Yeimy A., Rocha, Cristina M. R., Teixeira, José A., and Orrego, Carlos E.
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- 2020
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9. Ulva rigida Valorization into Poly(3-hydroxybutyrate), Organic Acids and Functional Ingredients
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Leandro, Tânia, primary, Teles, Marco, additional, Gomes-Dias, Joana S., additional, Marques, Mafalda, additional, Rocha, Cristina M. R., additional, da Fonseca, M. Manuela R., additional, and Cesário, M. Teresa, additional
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- 2023
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10. Postharvest Quality Improvement of Tomato (Solanum lycopersicum L.) Fruit Using a Nanomultilayer Coating Containing Aloe vera.
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Flores-López, María L., Vieira, Jorge M., Rocha, Cristina M. R., Lagarón, José M., Cerqueira, Miguel A., Jasso de Rodríguez, Diana, and Vicente, António A.
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TOMATOES ,ALOE vera ,FRUIT ,PRESERVATION of fruit ,SURFACE coatings ,ETHYLENE synthesis - Abstract
The effectiveness of an alginate/chitosan nanomultilayer coating without (NM) and with Aloe vera liquid fraction (NM+Av) was evaluated on the postharvest quality of tomato fruit at 20 °C and 85% relative humidity (RH) to simulate direct consumption. Both nanomultilayer coatings had comparable effects on firmness and pH values. However, the NM+Av coating significantly reduced weight loss (4.5 ± 0.2%) and molds and yeasts (3.5–4.0 log CFU g
−1 ) compared to uncoated fruit (16.2 ± 1.2% and 8.0 ± 0.0 log CFU g−1 , respectively). It notably lowered O2 consumption by 70% and a 52% decrease in CO2 production, inhibiting ethylene synthesis. Visual evaluation confirmed NM+Av's efficacy in preserving the postharvest quality of tomato. The preservation of color, indicated by the Minolta color (a*/b*) values, demonstrated NM+Av's ability to keep the light red stage compared to uncoated fruit. The favorable effects of NM+Av coating on enhancing postharvest quality indicates it as a potential alternative for large-scale tomato fruit preservation. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
11. Ohmic heating-based extraction of biocompounds from cocoa bean shell
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Sánchez, M., Santos, Pedro Miguel Ferreira, Dias, Joana Sofia Gomes, Botelho, C. M., Laca, A., Rocha, Cristina M. R., and Universidade do Minho
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Ohmic heating ,Green technology ,Cocoa bean shell ,Antioxidants ,Phenolic compounds - Abstract
Cocoa bean shell (CBS), a by-product of the chocolate industry, was employed as substrate for the sustainable recovery of bioactive compounds using ohmic heating (OH). Total phenolic content and antioxidant activity of the treated CBS were optimized by experimental design. Maximum extraction of antioxidant phenolic compounds (23mg GAE/g CBS) was obtained at 67°C, 50min and 44% ethanol (v/v). The antioxidant activity of the extracts obtained under of the central point conditions was 284.5M Fe2+/g extract (FRAP) and 36.4MTE/g extract (DPPH). The use of OH increased the extraction of bioactive phenolic compounds when compared to the conventional process (CH) (approximately 40%). An increased on the chemical antioxidant activity was also observed, ranging from 4 to 20%. The metabolic activity of the extracts obtained by the two methods (OH and CH) was evaluated in non-tumoral (HEK293T and L929) and tumoral cell lines (Caco-2, HT-29, and HeLa). The CBS extracts presented low toxicity in non-tumoral cells and ROS preventive effects. These characteristics make them ideal to be used in food processing and formulation, as well as nutraceutical products due to their antioxidant protection. The use of OH results in an extract with higher phenolic content and higher antioxidant activity and low environmental impact., This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of both the strategic funding of UIDB/04469/2020 unit and LA/P/0029/2020 (LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems), and by the Project SV-PA-21-AYUD/2021/51041 from the Economy and Employment Office of Principality of Asturias (Spain). J. S. G.-D. acknowledges the PhD scholarship 2021.06136.BD provided by FCT. Pedro Ferreira-Santos would like to express gratitude to the Spanish Ministry of Science, Innovation and Universities for financial support (FJC2021-046978-I)., info:eu-repo/semantics/publishedVersion
- Published
- 2023
12. Circular economy challenges in bioresources valorization - the role of microorganisms
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Rocha, Cristina M. R. and Universidade do Minho
- Abstract
ln a world that is expected to face acute resource shortage in the near future, exploring the possibility of fully using the available biomass and significantly improving the recovery of relevant nutrients/compounds are strategies of the utmost importance. Holistic strategies supporting a circular economy, enabling the sustainable and efficient use of available feedstocks, while targeting minimal environmental impact and zero wastes, are in order. ln this context, selecting efficient strategies, aiming at recovering the maximum number of fractions while simultaneously allowing for high yields and functionality, economic feasibility, environmental sustainability, and holistic integration in biorefinery approaches, is a challenging task. Biorefineries were initially designed to provide energy and chemicals from lignocellulosic biomasses using fermentation processes. The concept can be extended to other types of under-used bioresources, including food wastes or marine biomasses. However, bottlenecks include the need for processes that can cope with different feedstocks and variable feedstock quality. ln fact, improved enzymes and microorganisms are needed, resilient to these highly variable feedstocks and resistant to high(er) amounts of common inhibitors. Microorganisms capable of metabolizing specific compounds, such as oils or marine polysaccharides, into high-value products or ingredients would also allow broadening the range of biomasses that can be included in these circular economy approaches. Simpler valorisation strategies may include to enrich the biomass in different microbial metabolites or in single cell protein. Besides their role as high value commodities producer, microorganisms can also be an important processing aid. For instance, they may be used a stabilizing agent or to degrade packaging. Summing up, microorganisms can be key players in adding value to under-used bioresources by providing feasible processes to convert these bioresources into different high-value products, thus increasing economy circularity., info:eu-repo/semantics/publishedVersion
- Published
- 2023
13. Electric fields as a promising technology for the recovery of valuable bio compounds from algae: Novel and sustainable approaches
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Pereira, Sara Gonçalves, Pereira, Ricardo Nuno Correia, Rocha, Cristina M. R., Teixeira, J. A., and Universidade do Minho
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Macroalgae ,Sustainability ,Ohmic heating ,Microalgae ,Extraction - Abstract
The development of more efficient and sustainable technologies for the recovery of valuable compounds holds the ability to change the paradigm of the use of resources. Electric field-based technologies have been regarded as highly promising towards an efficient extraction of biometabolites while addressing sustainability. Algal biomass is a potential valuable resource. Besides its nutritional value, it provides a wide range of bio compounds with a variety of industrial applications in different sectors (e.g., food, feed, cosmetics). Implementing a cascade biorefinery approach based on electric-fields application would boost the production of high-value-added products from algal biomass, opening up new opportunities for the development of a flourishing blue bioeconomy. This review paper provides an overview of the use of alternative extraction technologies in algae biomass, focusing mainly on electric field-based methodologies, suggesting cascade biorefinery approaches considering different alternative technologies depending on the target fraction, and envisaging their environmental sustainability and economic feasibility., This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2020, LABBELS - Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020, and the PhD grant reference 2021.07623.BD (Sara G. Pereira). Ricardo N. Pereira acknowledge FCT for its Assistant Research program under the scope of Scientific Stimulus Employment with reference CEECIND/02903/2017., info:eu-repo/semantics/publishedVersion
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- 2023
14. Solubilization and Hydrolysis of Porcine Coagulated Blood Protein Using Sub-Critical Solvent Extraction
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Marques, Bianca, primary, Nunes, Rafaela, additional, Araújo-Rodrigues, Helena, additional, Pintado, Manuela, additional, Pereira, Ricardo N., additional, Teixeira, José A., additional, and Rocha, Cristina M. R., additional
- Published
- 2023
- Full Text
- View/download PDF
15. Brewer's yeast as a clean label ingredient for replacement of phosphates in hams
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Tomaz, Samuel A., Machado Jr, Júlio C., Dias, Joana Sofia Gomes, Pereira, Ricardo Nuno Correia, Rocha, Cristina M. R., Ferreira, Isabel M. P. L. V. O., and Universidade do Minho
- Abstract
Phosphates are common additives in the meat industry. They are used to increase the water retention capacity. They act in buffering, emulsification, colour stability, inhibition of lipid oxidation, antibacterial activity and protein dispersing properties. Excessive consumption inhibits absorption of minerals, causes kidney diseases and colorectal cancer. Brewer's yeasts are sets of strains responsible for beer fermentation. Often Saccharomyces cerevisiae, which consume sucrose, maltose and maltotriose, producing ethanol and CO². Studies show that brewer's yeast as a meat food ingredient may produce the same functions as phosphate. In this study we evaluated the potential use of brewers yeast to substitute phosphate in meat products. Hams with brewers yeasts extracts were evaluated for appearance and texture properties. Comparing with control samples, with and without phosphates, and three commercial phosphate substitutes. The impact of different treatments on colour was evaluated by the difference (Delta E test) between two colours in a L*a*b* colour space. The texture properties: hardness, cohesiveness, elasticity, gumminess, chewiness and resilience were evaluated by the TPA test (Texture Profile Analysis) of double compression. Extracts were obtained through hydrothermal treatment at high temperatures, using an autohydrolysis reactor in different temperatures and extraction times (i) only with water and solvent and (ii) with sodium hydroxide as a solvent at two concentrations. They were tested at 6.5 g/kg, the phosphate concentration in the model matrix, and 13.3 g/kg, the maximum concentration indicated to be added of the commercial phosphate substitutes tested. Hams produced with brewers yeasts extracted with NaOH 1M showed no significant differences (p, info:eu-repo/semantics/publishedVersion
- Published
- 2023
16. The use of subcritical water extraction for the recovery of protein from sardine residues
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Gonçalves-Lima, Beatriz, Marques, Bianca, Teixeira, J. A., Rocha, Cristina M. R., and Universidade do Minho
- Abstract
Nowadays, due to the growing world population, we face sustainability issues caused by animal protein shortage and environmental impact of animal protein-based industries [1]. This rose the need of full usage of high-quality protein sources, capable of being produced on a commercial scale and minimizing environmental impact. The design of extraction methodologies to recover protein from underused biomasses, using sustainable approaches, emerges to create value- added ingredients for food and feed applications, easing these sustainability problems. The present work aimed to recover and solubilize protein-rich fractions from sardine heads residues, using subcritical water extraction. Extractions were performed at temperatures ranging from 150 to 250 oC. Further, a sequential treatment with two steps was also tested: the first one at 120 oC, to recover soluble protein, followed by a second one at 180 oC, to hydrolyse and solubilise the remaining insoluble protein. The protein fractions were analysed for their lipid, protein and ash contents, and their potential antioxidant activity with colorimetric assays. For protein quantification, sardine residues samples were freeze-dried and analysed by Kjeldahl and the protein solubilized fractions were analysed by Bradford. The ash content was obtained with a muffle furnace and the lipid content by the Bligh and Dyer method. The protein solubilization of the fractions and their protein content increased with the increase of temperature. Moreover, the colorimetric assays revealed that all samples presented antioxidant activities, being the 250 oC sample the one presenting the highest antioxidant activity. Further, the sequential process allowed the recovery of two fractions with different functionalities. Taken together, the use of subcritical water extraction has proved to be an efficient and sustainable method to recover and solubilize protein from sardine residues, and that these residues have great potential to be included in food and feed formulation as a protein-rich and bioactive fraction., This work was financially supported by the project BioEcoNorte - Water and nutrients management based on BIOrefinery and circular ECOnomy towards a sustainable agri-food system of the NORTE of Portugal (NORTE-01-0145-FEDER-000070) co-funded by CCDR-N - Comissão de Coordenação e Desenvolvimento Regional do Norte, Portugal 2020, Norte 2020, and the European Union through the European Structural and Investment Funds (ESIF). The work was also supported by the Portuguese Foundation for Science and Technology (FCT), under the scope of the strategic funding of the UID/BIO/04469/2020 unit and LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechnaical Systems, LA/P/0029/2020., info:eu-repo/semantics/publishedVersion
- Published
- 2023
17. Production of bioactive protein-enriched hydrolysates from fish by-products using autohydrolysis
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Marques, Bianca, Ribeiro, Tânia, Pintado, Manuela, Teixeira, J. A., Rocha, Cristina M. R., and Universidade do Minho
- Abstract
Protein demand is expected to double by 2050 [1]. The amount of by-products generated from the fish processing industry represents more than half of the entire fish weight. These materials are commonly discarded with high costs and environmental burden [1,2]. Giving their high protein content (57%, dry weight), fish- based by- and co-products could be used in the development and fortification of food and feed products, thus contributing to bioeconomy and sustainability. Hydrolysis has been widely used to extract protein from several matrixes and the resulting hydrolysates usually exhibit interesting bioactive and functional properties [3,4]. The present work aimed the production of bioactive protein-enriched hydrolysates from fish by-products, using water under subcritical conditions. For that, the raw material was homogenized and centrifugated at 8000 g and 4 ºC for 30 min. The resulting solid biomass was used for the hydrolysis experiments. Several temperatures (150 to 230 ºC) and times (5 to 30 min) were tested, using 20 g (dry weight) of biomass and 0.4 L of water. After the hydrolysis, the solubilization yields were determined and the hydrolysates were analysed for their chemical composition, peptide profiles (HPLC) and antioxidant activity (in vitro colorimetric assays). The solubilization yields increased with temperature and time of reaction. All hydrolysates presented antioxidant activity, being the best conditions at temperatures between 170 and 230 C. Moreover, all hydrolysates presented high protein content (up to 82%). Autohydrolysis proved to be an efficient method for the solubilization and hydrolysis of protein from fish by-products without the need for chemicals or expensive enzymes. Antioxidant hydrolysates with high protein content were produced, which could be useful in the development of and fortification of food and feed products and could be a possible path to a rational and integral use of scarce protein sources in the near future., The authors would like to thank Sebol - Comércio e Indústria de Sebo S.A. for providing the fish by-products. This work was financially supported by Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2020 unit Further support was obtained by Portugal 2020, European Structural and Investment Funds (ESIF) and Agência Nacional de Inovação, S.A. (ANI) under the scope of the project HeathyPETFOOD- PetFood formulations for promotion of health and quality of life (POCI-01-0247-FEDER-047073)., info:eu-repo/semantics/publishedVersion
- Published
- 2023
18. Enzymatic approach for the extraction of bioactive fractions from red, green and brown seaweeds
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Teixeira, Catarina Isabel Guedes, Dias, Joana Sofia Gomes, Cunha, Sara A., Pintado, Manuela E., Pereira, Ricardo Nuno Correia, Teixeira, J. A., Rocha, Cristina M. R., and Universidade do Minho
- Subjects
seaweeds ,Science & Technology ,bioactive compounds carbohydrases ,extraction ,proteases - Abstract
Although several enzymatic approaches have been applied with this intent, the sequential use of enzymes covering both cellulolytic and proteolytic activity has never been performed in seaweeds. Sequential use of these enzymes improved the overall extraction yield by up to 160%, 30% and 80% in the different seaweeds when compared to the control condition (water extraction), use of a carbohydrases cocktail alone and use of proteases alone, respectively. Regarding the proximate composition of extracts, it proved to be an efficient approach for the solubilization of carbohydrates (up to 28% in G. vermiculophylla, 66% in P. dioica, 77% in U. rigida and 35% in F. vesiculosus) and protein (up to 55% in G. vermiculophylla, 47% in P. dioica, 52% in U. rigida and 42% in F. vesiculosus). For all biomasses, the combination of enzymes induced a significant increase in antioxidant activity, not only by the increase of phenolic compounds but also by the hydrolysis of protein to peptides. Moreover, extracts from red seaweeds displayed prebiotic activity which can be ascribed to their increased content in oligosaccharides and protein/peptides. Overall, the sequential use of enzymes with different activities demonstrated to be an efficient approach for extracting functional fractions to be used as functional ingredients to improve the nutritional value (e.g. in proteins) and/or to include antioxidant and prebiotic features in the food product., This work was supported by the Portuguese Foundation for Science and Technology (FCT), under the scope of the strategic funding of UID/BIO/04469/2020 unit and LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020, by the project VALORMAR - Full valorization of marine resources: potential, technological innovation and new applications (call 10/SI/2016, reference 24517- Mobilizing R&TD Programs) co-funded by Compete 2020, Lisboa 2020, Algarve 2020, Portugal 2020 and European Union, through the European Regional Development Fund (ERDF) and by the project Bioeconorte (ref. NORTE-01-0145-FEDER-000070; co-funded by ERDF, through Norte2002). J. S. G.-D. and S.A.C. acknowledge the doctoral fellows 2021.06136. BD and SFRH/BD/144155/2019, respectively, provided by FCT. R. N. P. acknowledges FCT for its Assistant Research program under the scope of Scientific Stimulus Employment with reference CEECIND/02903/2017., info:eu-repo/semantics/publishedVersion
- Published
- 2023
19. Active aroma compounds assessment of processed and non-processed micro- and macroalgae by solid-phase microextraction and gas chromatography/mass spectrometry targeting seafood analogs
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Moreira, Catarina, Santos, Pedro Miguel Ferreira, Teixeira, J. A., Rocha, Cristina M. R., and Universidade do Minho
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algae ,Science & Technology ,sensory evaluation ,solid-phase microextraction ,seafood processing ,volatile compounds ,GC-MS ,sustainability ,odorants - Abstract
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/ fsufs.2022.1011020/full#supplementary-material, The odor of four algae was investigated and compared to evaluate the potential of these algae to mimic shrimp aroma. Solid-phase microextraction followed by gas chromatography analysis coupled with sensory analysis was used for performance assessment. The volatile organic compounds were determined in non-processed, raw samples (r), and processed cooked (c) and cooking water (w) samples for two microalgae [Nannochoropsis oceanica (NO) and Tetraselmis chuii (TC)], two macroalgae [Ulva rígida (UR) and Saccharina latíssima (SL)], and shrimp Vannamei cong (SH). The results showed significant differences in the composition of volatile compounds between macroalgae and microalgae. The key odorants in macroalgae were octanal, 2-octenal, nonanal, and β-ionone, and in microalgae were 1,5-octadien-3-ol, hexanal, 2,4-decadienal, 2-octenal, octanal, nonanal, 3,5-octadien-2-one, and terpenes. The PCA analysis of GC-MS data showed odor similarities between the studied samples, which were divided into five main groups: (1) TC(c) and TC(w); (2) TC(r) and NO(c); (3) NO(r), NO(w), and SL(w); (4) SL(c), UR(r), UR(c), and UR(w); and (5) SL(r). The data from the sensory analysis show bigger similarities between the macroalgae and the shrimp odor. Overall, the data provided indicate that the cooking water and cooked samples are very similar in key components of odorants. These features allow the possibility to use algae and their processed resulting products as a shrimp flavor replacement in non-animal-based food formulations, thus decreasing the pressure on seafood crops and aquaculture-associated issues leading to more sustainable livestock. Furthermore, circularity and waste reduction may be further enabled by the use of otherwise wasted cooking water as an odorant agent., This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and by LABBELS— Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020. This study was also funded by the ESIF—European Structural and Investment Funds under the scope of the projects ALGAVALOR—Microalgae integrated production and valorization of its various applications (ref. POCI-01-0247-FEDER-035234; LISBOA-01-0247-FEDER-035234; ALG-01-0247-FEDER-035234; through Portugal 2020), and BioEcoNorte project (ref. NORTE-01-0145-FEDER-000070, through Portugal 2020 and Norte2020). CM is recipient of a Ph.D. scholarship supported by FCT (Ref. 2021.05734.BD)., info:eu-repo/semantics/publishedVersion
- Published
- 2022
20. Active aroma compounds assessment of processed and non-processed micro- and macroalgae by solid-phase microextraction and gas chromatography/mass spectrometry targeting seafood analogs
- Author
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Moreira, Catarina, primary, Ferreira-Santos, Pedro, additional, Teixeira, José António, additional, and Rocha, Cristina M. R., additional
- Published
- 2022
- Full Text
- View/download PDF
21. Electro-based technologies for the extraction of phenolic compounds
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Rodrigues, Rui Miguel Martins, Genisheva, Zlatina, Santos, Pedro Miguel Ferreira, Rocha, Cristina M. R., Pereira, Ricardo Nuno Correia, Vicente, A. A., and Universidade do Minho
- Subjects
Electric fields ,Electroporation ,Antioxidant activity ,Waste ,Microalgae ,Organic compound ,Agricultural products ,Thermal permeabilization ,Anticancer activity - Abstract
During the past decade, technologies based on the application of electric fields emerged in different areas of biotechnology. Processing strategies based on the application of pulsed electric fields (PEF), moderate electric fields (MEF), and high voltage electrical discharges (HVED) in combination with their inherent thermal effectsi.e., Ohmic heating (OH) or pulsed Ohmic heating (POH)have been used to extract bioactive compounds from underrated agro-food and forestry materials, as well as from biological biomass (such as seaweeds and microalgae, for example). Recently, extraction of phenolic compounds has received increasing attention from the research community. Several classes of phenolic compounds (either simple or complex) can be found in plants, fruits, and vegetable. Polyphenols (PPs) such as curcumin, anthocyanin rich extracts from grapes, and catechin rich extracts from plants present interesting biological and functional properties and are attractive due to their potential health benefits, which can include antioxidant, antibacterial, anticancer, and antiinflammatory activities. Those molecules are also well recognized as organoleptic food enhancers, affecting color and taste properties. This chapter intends to systematically review the use of electro-based technologies in extraction of PPs addressing the most promising and successful extraction strategies, as well as to identify phenolic molecules that can present more interesting properties after their retrieval for a potential route of valorization., Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. FCT and the European Fund for Regional Development (FEDER) and COMPETE 2020— Competitiveness and Internationalization Operational Program, under the scope of project OH2O (PTDC/ EQU-EQU/29145/2017; POCI-01-0145-FEDER-029145). ERDF Funds through the Competitiveness factors Operational program—COMPETE and by National Funds through the FCT under the project AgriFood XXI (NORTE- 01-0145-FEDER-000041)., info:eu-repo/semantics/publishedVersion
- Published
- 2022
22. Sambucus nigra flower and berry extracts for food and therapeutic applications: effect of gastrointestinal digestion on in vitro and in vivo bioactivity and toxicity
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Santos, Pedro Miguel Ferreira, Nogueira, António, Rocha, Cristina M. R., Pereira-Wilson, Cristina, Teixeira, J. A., Botelho, C. M., and Universidade do Minho
- Subjects
Science & Technology - Abstract
The bioavailability of natural compounds should be assessed through different perspectives. Studying the behaviour of the extracts after digestion is often overlooked but is crucial for success in the development of active food ingredients. Thus, the bioaccessibility of S. nigra (flower and berry) extracts after in vitro gastrointestinal digestion and their effect on toxicity and bioactive potential were studied. The flower extract had a higher content of phenolic compounds, like rutin, chlorogenic acid and rosmarinic acid, while in the berry extract, rutin, resveratrol, ferulic acid and chlorogenic acid were the main phenolic compounds. The effect of the non-digested and digested extracts was significantly different on different cell lines. The IC50 of the normal cell line (L929) was the highest, indicating low toxicity. The IC50 of the cancerous cell lines (HeLa and HT29) was lower, particularly the extract obtained from the flower upon digestion. In the presence of an oxidant agent tbHP, only the berry extract was able to significantly reduce the formation of ROS in the L929 cell line, while in the HeLa cells, all the extracts were able to reduce ROS formation. The in vivo Artemia salina lethality bioassay demonstrated a dose-dependent effect of extracts, and the berry digested extract induced the lowest mortality rate. The promising results obtained on the chemical and biological evaluation of the extracts indicate that the natural compounds isolated from S. nigra by-products can be used as potential ingredients for functional food formulations and/or as bio-therapeutic agents., This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit, by program Marie Skłodowska-Curie grant (MSCA-RISE; FODIAC; 778388), and by the European Regional Development Fund (ERDF) through the Competitiveness factors Operational program – Norte 2020, COMPETE and by National Funds through the FCT – under the project AgriFood XXI (NORTE- 01-0145-FEDER-000041)., info:eu-repo/semantics/publishedVersion
- Published
- 2022
23. Effect of Ohmic Heating on the Extraction Yield, Polyphenol Content and Antioxidant Activity of Olive Mill Leaves
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Safarzadeh Markhali, Fereshteh, primary, Teixeira, José A., additional, and Rocha, Cristina M. R., additional
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- 2022
- Full Text
- View/download PDF
24. Recent advances in the valorization of algae polysaccharides for food and nutraceutical applications: a review on the role of green processing technologies
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Dias, Joana Sofia Gomes, Teixeira, J. A., Rocha, Cristina M. R., and Universidade do Minho
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Prebiotics ,Science & Technology ,Macroalgae ,Microalgae ,Environmentally friendly processing ,Bioactive compounds - Abstract
Recently, there are two main movements gaining public attention: a ``green revolution'' focused on the use of sustainable processes and the increasing concerns with health-promoting diets and the recognition of the gut microbiota and its effects on the treatment and prevention of several diseases. Microalgae and macroalgae arise as an interesting alternative to obtain these nutraceutical compounds in a sustainable way, due to their extensive list of possible applications (mainly due to their tremendous variation in composition), advantages over terrestrial biomasses and other nutritional sources, and ancestral food consumption history. Nevertheless, available literature has been more focused on the lipidic and protein-rich fraction of microalgae and the texturizing aspects of the carbohydrate-fraction from macroalgae, overlooking their use as prebiotic or nutraceutical ingredients. Furthermore, when these uses are studied, it is with a focus on the action mechanisms of the carbohydrate-rich fractions on gut microbiota and on conventional or, at most, enzyme-assisted ways of obtaining such bioactive fractions. The present review aims at providing an integrated overview of the current knowledge regarding the link between green extraction technologies and the bioactive properties of obtained fractions. In here, the composition of most common algae polysaccharides as well as strategies to increase their relative content in the biomass is discussed. Furthermore, the influence of several environmentally friendly technologies (enzymes, microwaves, ultrasounds, sub and supercritical fluids) and their influence on the polysaccharides' structure and bioactivity are reviewed. Despite the emphasis on prebiotic potential and carbohydrate valorization, several examples of other bioactive fractions are explored., This work was supported by the Portuguese Foundation for Science and Technology (FCT), under the scope of the strategic funding of UID/BIO/04469/2020 unit and scholarship 2021.06136.BD. This study was also supported by cLabel + — Innovative natural, nutritious, and consumer-oriented clean label foods (ref. POCI-01–0247- FEDER-046080, funded by FCT and by the European Union, under the scope of COMPETE2020, PORTUGAL2020 and LISBOA2020)., info:eu-repo/semantics/publishedVersion
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- 2022
25. Analysis of the profile of volatile organic compounds in Chlorella vulgaris
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Moreira, Catarina, Santos, Pedro Miguel Ferreira, Nunes, R., Teixeira, J. A., Rocha, Cristina M. R., and Universidade do Minho
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microalgae ,SPME ,VOCs ,Aroma characterization - Abstract
It is known that the incorporation of microalgae into foodstuffs enables to modify the overall flavor leading to desirable or undesirable organoleptic properties. Nevertheless, few were the studies conducted focusing on the analysis of the profile of volatile organic compounds (VOCs) produced by microalgae, which are the main compounds responsible for the aroma perception. The aim of this study was to analyze the VOCs profile of Chlorella vulgaris powder by gas chromatography coupled with mass spectrometry (GC-MS). Different techniques were used for the extraction of the VOCs, prior to the chromatographic analysis: 1) solid-phase microextraction (SPME), and 2) ultrasound-assisted liquid extraction (UALE) using five different solvents. For the SPME analysis, 0.5 g of C. vulgaris was exposed to a divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber and was desorbed for 5 min into GC-MS. For the UALE, 2 g of sample was placed in five different solvents for extraction (chloroform, dichloromethane, hexane, ethyl acetate and acetone). For both SPME and UALE the GC-MS analysis was performed on Stabilwax (SW) and RXI-5 columns. The results showed very different profiles of VOCs between columns and techniques of extractions. In the analysis with the SW column no compounds were detected in acetone, hexane and ethyl acetate and only a few were present with the chloroform (39 VOCs) and dichloromethane (15 VOCs) extraction when compared to SPME (75 VOCs). In the RXI-5 analysis, a reduced number of compounds was identified when comparing with SW in SPME (39 and 75 VOCs, respectively), and in UALE, ethyl acetate was the only solvent with any retention of VOCs that could be detected with this column, having chloroform, dichloromethane, acetone and hexane, 10, 10, 5, and 8 VOCs respectively. In SPME, the main chemical classes of identified VOCs were, alcohols and hydrocarbons. In UALLE, the VOCs profile was more balanced with alcohols, aldehydes, carboxy group, ketones and hydrocarbons. Only with hexane it was possible to find aromatic groups. Compounds such as hexanal, tetradecane, and 3-methylbutanal were found in SPME as reported in literature, although 3-methylbutanal was only present for SW. In conclusion, SPME run in a SW column showed more VOCs retention and seems to be more appropriate to enable aroma prediction in C. vulgaris-based foodstuff., Portuguese Foundation for Science and Technology (FCT) - 2021.05734.BD, info:eu-repo/semantics/publishedVersion
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- 2022
26. Production of lipid rich-extracts from Chlorella vulgaris using ohmic heating
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Madureira, Leandro, Avelar, Zita, Conde, Tiago, Couto, Daniela, Domingues, M. Rosário, Domingues, Pedro, Pereira, Ricardo Nuno Correia, Rocha, Cristina M. R., Teixeira, J. A., and Universidade do Minho
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Microalgae ,Ohmic Heating ,Chlorella vulgaris ,PUFAs rich-extracts - Abstract
Microalgae biomass is a promising raw material for several bioproducts suitable for food, energy and pharmaceutical industries. The aim of the present work was to optimize the extraction of bioactive compounds from Chlorella vulgaris using Ohmic Heating (OH). A rotatable central composite design for two factors was used to assess the effects of temperature and solvent (% ethanol in water) on lipid extraction yields and fatty acid profile. OH extraction experiments were powered by low-frequency (50 Hz) and high-frequency (25 kHz) to identify the presence of non-thermal effects and its influence on composition and bioactive properties of the generated extracts. Lipid extraction using OH was successfully optimized with the best extraction conditions found at 70 ºC for 5 min using 88 % ethanol as a solvent. No effect on the application of the electrical frequencies was observed. These conditions allowed to recover up to 87 % of lipids from biomass, polyunsaturated fatty acids (PUFAs) accounting for 43 % of the extracted lipids against 26 % of saturated fatty acids (SFAs). The fatty acid profile reveals that C16:2, C16:3, C18:2 and C18:3 correspond to the PUFAs extracted from Chlorella vulgaris. Regarding lipid extraction yields OH was statistically equivalent to conventional heating (COV); however, in terms of heating kinetics, OH reaches the required extraction temperature 5 times faster than COV. Results showed that OH has potential to be applied as a treatment for the production of Chlorella vulgaris PUFAs richextracts providing high recovery yields with reduced treatment times and less energy consumption., This study was supported by: the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and project OH2O – PTDC/EQU-EQU/029145/2017; by FEDER funds through COMPETE2020 – Programa Operacional Competitividade e Internacionalização (POCI) under the scope of Project Algavalor (POCI-01-0247-FEDER-035234; LISBOA-01-0247-FEDER-035234; ALG-01-0247-FEDER-035234) and OH2O (POCI-01-0145-FEDER-029145); AgriFood XXI R & D & I project, operation number NORTE-01-0145-FEDER-000041, co-financed by the European Regional Development Fund (FEDER) through NORTE 2020 (Northern Regional Operational Program 2014/2020). Ricardo N. Pereira acknowledge FCT for its Assistant Research contract obtained under CEEC Individual 2017. Leandro Madureira acknowledges FCT for its PhD fellowship (SFRH/BD/151474/2021) obtained under MIT Portugal Program, info:eu-repo/semantics/publishedVersion
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- 2022
27. Gastrointestinal digestion of Sambucus nigra L. flower and berry bioactive molecules: in vitro and in vivo evaluation
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Santos, Pedro Miguel Ferreira, Nogueira, António, Rocha, Cristina M. R., Teixeira, J. A., Botelho, C. M., and Universidade do Minho
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Toxicity ,Antioxidant activity ,Sambucus nigra ,Bioaccessibility ,Gastrointestinal digestion ,Phenolic compounds - Abstract
Sambucus nigra L. is a flowering plant, which has been used for centuries as part of folk medicine, due to its bioactive properties. In this work the bioaccessibility of S. nigra (flower and berry) extracts after in vitro gastrointestinal digestion and their effect on toxicity and bioactive potential were studied. The extractions were performed with distilled water at 90 °C for 30 min. The obtained extracts were exposed to the INFOGEST harmonized in vitro digestion protocol. The chemical composition was analyzed before and after the digestion process, in terms of phenolics (TPC), flavonoids (TFC), and anthocyanins; additionally, phenolic compounds profile was determined by UPLC. The antioxidant activity was determined by FRAP, DPPH and ABTS methods. Cell viability and cellular reactive oxygen species (ROS) levels were assessed using normal cell line (L929) and cancerous cell lines (HeLa and HT29). The in vivo toxicity was measured by a simple model of Artemia salina. The chemical analysis revealed that berries evidence higher content of total extractives than the flower. When comparing the extracts before and after digestion there is an increase in the TPC on both extracts, while a significant decrease on the TFC. The compounds with the higher content were rutin, chlorogenic acid and rosmarinic acid in S. nigra flower extract, while in the S. nigra berry extract were rutin, resveratrol, ferulic and chorogenic acid. The effect of the non-digested and digested extracts was significantly different on the different cell lines. The IC50 of L929 cells was the highest, indicating low toxicity. The IC50 of HeLa and HT29 cells was lower, particularly the extract obtained from the flower upon the digestion. These results indicate that these natural compounds can be used as adjuvants in anticancer therapy. In the presence of an oxidant agent tbHP, only the berry extract was able to significantly reduce the formation ROS in L929 cell line, while on the HeLa cells all the extracts were able to reduce it. The in vivo assay demonstrated a dose-dependent effect for all extracts. The berry digested aqueous extract induced the lowest mortality rate in all concentrations evaluated. The promising results obtained on the chemical and biological evaluation of the extracts indicate that the natural compounds isolated from S. nigra by-products can be used as potential ingredients for functional food formulations, and/or as bio-therapeutic agents., This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit, by program Marie Skłodowska-Curie grant (MSCA-RISE; FODIAC; 778388), and by the European Regional Development Fund (ERDF) through the Competitiveness factors Operational program – Norte 2020, COMPETE and by National Funds through the FCT - under the project AgriFood XXI (NORTE- 01-0145-FEDER000041)., info:eu-repo/semantics/publishedVersion
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- 2022
28. Osborne fractionation: an approach for red macroalgae proteinassessment
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Nunes, R., Santos, Pedro Miguel Ferreira, Moreira, Catarina, Teixeira, J. A., Rocha, Cristina M. R., and Universidade do Minho
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Macroalgae ,Protein ,Osborne - Abstract
Although animal sources enhance consumers' nutritional satisfaction, their cost, consumers dietary restrictions, as well as an increasing interest in vegetarianism, organic food and locally grown foods emphasizes the importance of finding new sustainable alternatives of protein sources in order to satisfy anticipated increased global needs without magnifying the negative impact. Porphyra dioica, Gracilaria verrucosa and Chondrus crispus are examples of red algae species that are used as a source of valuable compounds that can satisfy the demand for different foods and help to decrease the impact on animals use as the main food nutrients supplier. Since there are a wide range of different proteins in macroalgae, it is important to study and assess which kind of proteins are present in different species, which will help to predict if they can be used to replace some conventional animal proteins. The main objective of this work was the application of Osborne method for protein recovery from red algae species using different solvents (e.g., H2O, NaCl, EtOH and NaOH). The produced fractions were characterized in terms of extraction yields, protein content (Lowry method) and molecular weight by gel electrophoresis (SDS-PAGE). Results showed that the maximum protein content was obtained for Porphyra dioica and the minimum value for Chondrus crispus (i.e., 29.12 and 7.13 %, respectively). Regarding Osborne fractionation, all species showed higher protein content in water and NaOH resulting proteins fractions, being the best results associated with Gracilaria verrucosa, with a total protein extraction yield (gprotein/gbiomass) of 12.39 %. Comparing to the initial protein content of Gracilaria verrucosa (17.45 ± 0.05 %dw) it is possible extract 70.99 % of total protein where 35.83 % and 18.05 % corresponds to water and NaOH fractions, respectively. From the SDS-PAGE gels it is clearly visible that different species showed proteins with different molecular weights. Thus, it can be concluded that the Osborne method is a promising approach to characterize algal protein and can help choosing the best conditions for higher protein recovery from red macroalgae. The extracted proteins may be applied into different food prototypes in order to substitute conventional animal protein sources., Portuguese Foundation for Science and Technology (FCT) - UIDB/04469/2020 unit and SFRH/BD/07527/2020; BioEcoNorte project (NORTE-01-0145-FEDER-000070); NORTE 2020 (Programa Operacional Regional do Norte 2014/2020); Portugal 2020; ESIF- European structural and investment funds., info:eu-repo/semantics/publishedVersion
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- 2022
29. Rotondas red eggplant: nutritional characterization and extractionof high added value biocompounds using emerging electrotechnologies
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Santos, Pedro Miguel Ferreira, Duca, Anna, Genisheva, Zlatina, De Biasio, Filomena, Gorgoglione, Domenico, Rocha, Cristina M. R., Teixeira, J. A., and Universidade do Minho
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Ohmic heating ,Functional food ,Polyphenols ,Red Eggplant ,Antioxidants ,Biocompounds - Abstract
Rotondas Red Eggplant belongs to the family of Solanum aethiopicum species, cultivated in a specific area of Potenza (Basilicata, Italy) and has gained the Protected Designation of Origin certification. Recently, Red Eggplant was associated with different biological properties, related to health benefits, as antioxidant, hypoglycemic, antihypertensive and hypolipidemic, probably due to its chemical composition. The search for "greener" technologies has been one of the focus of research, having in mind industrial applications. In this sense, electrotechnologies, such as ohmic heating (OH), have been explored, showing to have potentially lower energy costs, higher extraction yields, being less time consuming and increasing the biocompounds chemical stability. The aim of this study was to determine the chemical/nutritional composition of Red Eggplant and to assess conventional and OH methods in the extraction and recovery of the main bio-compounds present in this material. Red Eggplant was characterized for the nutritional profile (AOAC procedures): proteins, carbohydrates, total extractives, lipids, ash and minerals. The selected conditions for the extraction of the main biocompounds, for conventional and OH methods, were: solid/liquid ratio 1:10 (w:v), 80 °C, 25 min and water or mixture of 50% (v/v) ethanol/water as a solvent. Subsequently, assays were carried out to determine the content of proteins (Bradford), total carbohydrates (phenolsulfuric acid) and phenolics (Folin-Ciocalteu), and antioxidant activity (FRAP and DPPH) of the obtained extracts. Monosaccharides profile after hydrolysis and phenolics were also quantified by HPLC. Results showed that carbohydrates were the major macronutrient, followed by protein, lipids and ash. K, Ca and Mg were the major minerals. Regarding the monosaccharide composition, glucose, fructose, xylose and arabinose were the most abundant sugars. Ethanolic extractives represented approximately 46 % of the Red Eggplant composition. Independently of the solvent, higher amounts of polyphenols, proteins and carbohydrates were extracted when OH was applied. Moreover, OH extracts showed greater antioxidant activity compared to extracts obtained by the conventional extraction method. According to the polyphenol profile of the extracts the main compounds found were taxifolin, rutin and rosmarinic acid. These results demonstrate the potential of this vegetable to be used for nutraceuticals or functional food., The authors wish to thank for the financial support to funding program Marie Skłodowska-Curie grant (MSCA-RISE; FODIAC; 778388). Pedro Santos is recipient of a fellowship supported by a doctoral advanced training (call NORTE-69-2015-15), funded by the European Social Fund under the scope of Norte2020 - Programa Operacional Regional do Norte (NORTE-08-5369-FSE-000036). Zlatina Genisheva is supported by the project OH2O (POCI-01-0145-FEDER-029145) funded by FCT and Fundo Europeu de Desenvolvimento Regional (FEDER) under the scope of Programa Operacional de Competividade e Internacionalizaçao (POCI)-COMPETE 2020 and Portugal 2020., info:eu-repo/semantics/publishedVersion
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- 2022
30. Screening of Phaeodactylum tricornutum extracts regarding their bioactive and functional properties
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Ferreira, Catarina, Teixeira, Catarina Isabel Guedes, Vicente, A. A., Rocha, Cristina M. R., and Universidade do Minho
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Functional properties ,Solvent extraction ,Microalgae biorefinery ,Phaeodactylum tricornutum ,Bioactive properties - Abstract
The expanding interest in addressing a more sustainable and eco-friendlier product development regarding the problem of ecological preservation results in a growing community search for bioactive natural-based formulations. Microalgae extracts potential for their interesting bioactive properties has been widely recognized, with antioxidant activity accounting for a major application in cosmetics, pharmaceutics and nutrition fields, due to its health-promoting effects. Moreover, microalgae generally contain large amounts of structural biopolymers, which might possibly display interesting rheological properties. This work was designed to enhance microalgae potential biotechnology exploration by attaining at least two different main fractions, namely with bioactive and texturizing functions. Phaeodactylum tricornutum was used under a biorefinery concept, by performing extractions with several solvent systems with a wide polarity spectrum. Previous work enhanced this microalgae species potential as an antioxidant agent, regardless of the antioxidant quantification method used, when compared to Nannochloropsis oceanica and Chlorella vulgaris extracts. P. tricornutum powdered biomass was extracted (4% dry weight) using water or hydroethanolic mixtures (25-96%) under same conditions of extraction, namely, over one hour at three different temperatures: 40ºC, 60ºC and 80ºC. All extracts were screened for their bioactive potential by three different antioxidant activity measurement assays: FRAP, ABTS and DPPH, as well as chemical characterized regarding their phenolic and pigment content. Lipidic fraction was evaluated for ethanol 25%, 50% and 96% extracts. Rheological properties and emulsifying capacity and stability were determined for water and ethanol 25% extracts, while protein and carbohydrate content were also assessed. Overall, findings from this study suggest that P. tricornutum extracts have a great potential for biotechnology purposes: aqueous extracts were particularly interesting for their functional properties while bioactive properties were more relevant for the ethanolic extracts. These may due to a higher protein and carbohydrate fraction present in more aqueous extracts. On the other hand, extracts with higher concentration of ethanol evidenced a greater amount of pigments, phenolics and lipids. In this manner, further studies should be fulfilled in order to explore their application in natural-based product formulation., This work was supported by the Portuguese Foundation for Science and Technology (FCT), under the scope of the strategic funding of UID/BIO/04469/2020 unit. This study has also received funding from the European Fund for Regional Development (FEDER), COMPETE 2020 – Competitiveness and Internationalization Operational Program (Portugal 2020) and the European Regional Development Fund (Algarve 2020 and Lisboa 2020), under the scope of the project AlgaValor (grant agreement nº POCI-01-0247-FEDER-035234; LISBOA-01-0247-FEDER-035234; ALG-01-0247-FEDER-035234)., info:eu-repo/semantics/publishedVersion
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- 2022
31. Unveiling the Antioxidant Therapeutic Functionality of Sustainable Olive Pomace Active Ingredients
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Quero, Javier, primary, Ballesteros, Lina F., additional, Ferreira-Santos, Pedro, additional, Velderrain-Rodriguez, Gustavo R., additional, Rocha, Cristina M. R., additional, Pereira, Ricardo N., additional, Teixeira, José A., additional, Martin-Belloso, Olga, additional, Osada, Jesús, additional, and Rodríguez-Yoldi, María Jesús, additional
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- 2022
- Full Text
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32. Pulsed electric fields for the extraction of proteins and carbohydrates from marine resources
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Pereira, Ricardo Nuno Correia, Avelar, Zita, Pereira, Sara Gonçalves, Rocha, Cristina M. R., Teixeira, J. A., and Universidade do Minho
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Electroporation ,Macroalgae ,Ohmic heating ,Electric field ,Microalgae ,Nonthermal effects - Abstract
Pulsed electric fields (PEF) is attracting great attention as nonthermal extraction technology on downstream processing of emergent marine bioresources such as micro- and macroalgae. These uni- and multicellular algae enclose a diverse and interesting biochemical composition and are recognized as important sources of valuable bioactive compounds and functional food nutrients. This chapter intends to give an overview about the role of electric field processing toward the extraction of proteins and carbohydrates given particular emphasis on PEF technology, addressing the latest scientific developments in the field. Electric field-based technologies have a great potential as a green tool, being an energy efficient and environmentally friendly processing technology, while reducing the operational time by allowing a strong electro permeation, that is electroporation of cellular membranes, that enhances the extraction yields while reducing the use of solvents. The possibility of combining PEF with other processing variables (i.e., ohmic heating) can bring more flexibility to the extraction process and will be highlighted., (undefined), info:eu-repo/semantics/publishedVersion
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- 2022
33. Sambucus nigra flower and berry extracts for food and therapeutic applications: effect of gastrointestinal digestion on in vitro and in vivo bioactivity and toxicity
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Ferreira-Santos, Pedro, primary, Nogueira, António, additional, Rocha, Cristina M. R., additional, Wilson, Cristina Pereira, additional, Teixeira, José A., additional, and Botelho, Cláudia, additional
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- 2022
- Full Text
- View/download PDF
34. Extracts From Red Eggplant: Impact of Ohmic Heating and Different Extraction Solvents on the Chemical Profile and Bioactivity
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Ferreira-Santos, Pedro, primary, Duca, Anna Barbara, additional, Genisheva, Zlatina, additional, Silva, Beatriz Nunes, additional, De Biasio, Filomena, additional, Botelho, Cláudia, additional, Rocha, Cristina M. R., additional, Gorgoglione, Domenico, additional, and Teixeira, José A., additional
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- 2021
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35. Chemical Characterization of Sambucus nigra L. Flowers Aqueous Extract and Its Biological Implications
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Ferreira-Santos, Pedro, primary, Badim, Helder, additional, Salvador, Ângelo C., additional, Silvestre, Armando J. D., additional, Santos, Sónia A. O., additional, Rocha, Sílvia M., additional, Sousa, Ana M., additional, Pereira, Maria Olívia, additional, Wilson, Cristina Pereira, additional, Rocha, Cristina M. R., additional, Teixeira, José António, additional, and Botelho, Cláudia M., additional
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- 2021
- Full Text
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36. Advances in Extraction Methods to Recover Added-Value Compounds from Seaweeds: Sustainability and Functionality
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Matos, Gabriela S., primary, Pereira, Sara G., additional, Genisheva, Zlatina A., additional, Gomes, Ana Maria, additional, Teixeira, José A., additional, and Rocha, Cristina M. R., additional
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- 2021
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37. Chapter 20 - Valorization of lignocellulosic-based wastes
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Romani, Aloia Perez, Rocha, Cristina M. R., Michelin, Michele, Domingues, Lucília, Teixeira, J. A., and Universidade do Minho
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Bioproducts ,Value-added compounds ,Emerging technologies ,Sustainable processes ,Biorefinery ,Lignocellulosic wastes - Abstract
The production of energy and chemicals from renewable biomass provides an ideal alternative to petroleum-based refinery reducing the dependence on fossil-fuel resources and contributing to the decarbonization and sustainable growth of the society. Lignocellulosic-based wastes have been recognized as the most important renewable source, not competing with food crops. In this sense, the valorization of lignocellulosic-based wastes for the production of value-added compounds contributes for achieving a circular economy, which aims to develop new income streams by the creation of novel sectors, adding value to products and promoting jobs. Lignocellulosic biomass is mainly composed by nonstructural components (such as extractives, ash, protein, and other minor components) and structural components (cellulose, hemicellulose, and lignin). The selective fractionation of lignocellulosic-based wastes allows the use of separate fractions for a suitable and specific application. This approach is known as lignocellulosic biorefinery. This chapter will approach the main commercial products obtained from lignocellulosic components and the biomass sources for the extraction of these fractions. Moreover, the most important applications of these biomass-derived products will be addressed, as well as some more recent techniques of processing for the extraction of extractives, cellulose, hemicellulose, and lignin., This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684/POCI01-0145-FEDER-007440), the BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020 e Programa Operacional Regional do Norte and the MultiBiorefinery project (POCI-01e0145-FEDER-016403). Michele Michelin is recipient of FCT fellowships (SFRH/BPD/100786/2014)., info:eu-repo/semantics/publishedVersion
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- 2020
38. Biocompounds recovery from Spirulina by conventional and ohmic heating methodologies: chemical and biological properties
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Ferreira-Santos, P., Nunes, R., Di Biasio, Filomena, Rocha, Cristina M. R., Gorgoglione, Domenico, Spigno, Giorgia, Teixeira, J. A., and Universidade do Minho
- Abstract
Extracting the totality of bio-compounds with industrial interest from Cyanobacterium is often prevented by the intrinsic rigidity of its cell wall. In this sense, the present study focuses on evaluating the influence thermal batch extraction (conventional extraction technologies) and ohmic heating (OH) assisted extraction (considered a greener alternative technology) in blue green microalgae Arthrospira platensis (Spirulina) cell disruption for bioactive fractions recovery. The proximal composition of Spirulina was initially determined. The maximum protein content (i.e., CPhycocyanin), total carbohydrates (TC) and total phenolic compounds (TPC) extracted in water at different times (30-120 min) and temperatures (30-51 ºC) was quantified after the conventional and OH- assisted extraction. The freeze-thawing process was used as control. The antioxidant activity (i.e., FRAP and DPPH assays) of the obtained extracts was assessed. Results showed that with the freeze-thawing process, traditionally used for the recovery of bio- compounds from Spirulina, the concentration of C-phycocyanin was approx. 42 mg/g of Spirulina, 26 mgGlcE/g Spirulina of TC and 9 mgGAE/g Spirulina of TPC. Using OH-assisted extraction, the maximum of C-Phycocyanin content obtained was 45 mg/g of Spirulina (obtained at 37 ºC, 30 min), the maximum carbohydrates content was 40 mgGlcE/g Spirulina and the maximum TPC was 10 mgGAE/g Spirulina. On the other hand, using conventional thermal treatment it can be observed that, under the same conditions, the bioactive compounds recovery decreased to 35 mg/g, 20 mgGlcE/g Spirulina for C-phycocyanin concentration and TC (p, info:eu-repo/semantics/publishedVersion
- Published
- 2019
39. Encapsulated Pine Bark Polyphenolic Extract during Gastrointestinal Digestion: Bioaccessibility, Bioactivity and Oxidative Stress Prevention
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Ferreira-Santos, Pedro, primary, Ibarz, Raquel, additional, Fernandes, Jean-Michel, additional, Pinheiro, Ana Cristina, additional, Botelho, Cláudia, additional, Rocha, Cristina M. R., additional, Teixeira, José António, additional, and Martín-Belloso, Olga, additional
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- 2021
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40. Olive Tree Leaves—A Source of Valuable Active Compounds
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Markhali, Fereshteh Safarzadeh, primary, Teixeira, José A., additional, and Rocha, Cristina M. R., additional
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- 2020
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41. Green and Sustainable Valorization of Bioactive Phenolic Compounds from Pinus By-Products
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Ferreira-Santos, Pedro, primary, Zanuso, Elisa, additional, Genisheva, Zlatina, additional, Rocha, Cristina M. R., additional, and Teixeira, José A., additional
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- 2020
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42. Influence of moderate electric fields on the performance of agars extraction from Gracilaria sp
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Rocha, Cristina M. R., Matos, G. S. S., Gonçalves, Raquel F. S., Pereira, Ricardo Nuno Correia, Teixeira, J. A., and Universidade do Minho
- Abstract
Seaweeds are important marine resources in Portugal, being mostly applied in the hydrocolloids industry or as food. Emerging applications include the production of high added-value bioactive compounds. Seaweed polysaccharides are water-soluble biopolymers and have a linear backbone composed of sugar units conjugated to form complex structures. The –OH groups, predominant in the polymeric chains can establish intra- and intermolecular hydrogen bonds which are excellent functional tools for gel and/or film-formation. Agar is extracted mainly from red seaweeds from Gelidiales and Gracilariales (in which group Gracilaria sp. are included). In the case of agar from Gracilaria sp., normally with inferior commercial characteristics, the functional properties are usually enhanced by an alkali pre-treatment that modifies the L-galactose-6-sulphate units into 3,6-anhydro-α-Lgalactose residues. Traditional hot water extraction is a tedious process, requires high solvent and energy consumptions and generates large amounts of waste creating the need for greener and more efficient technologies. The main objective of this work is to evaluate the influence of different electric fields intensity in the performance of the agar’s extraction from Gracilaria sp. in terms of yield, extraction time and extract’s quality., info:eu-repo/semantics/publishedVersion
- Published
- 2018
43. Moderate Electric Fields as a Potential Tool for Sustainable Recovery of Phenolic Compounds from Pinus pinaster Bark
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Ferreira-Santos, Pedro, primary, Genisheva, Zlatina, additional, Pereira, Ricardo N., additional, Teixeira, José A., additional, and Rocha, Cristina M. R., additional
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- 2019
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44. Alternative strategies for the extraction of compounds from natural resources
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Rocha, Cristina M. R., Pereira, Ricardo N., Ferreira-Santos, P., Ballesteros, Lina F., Michelin, Michele, Genisheva, Zlatina, Teixeira, J. A., and Universidade do Minho
- Abstract
Book of Abstracts of CEB Annual Meeting 2017, [Excerpt] Exploitable compounds from natural resources include polysaccharides, proteins and peptides, gum exudates, lipids, polyphenols and other secondary metabolites. Traditional-water extraction (TWE) of polysaccharides is a time-consuming process that requires high solvent and energy consumptions and generates large amounts of waste. Other bioactive compounds are traditionally extracted with organic solvents or mixtures of organic solvents and water, with or without heat. Subcritical water extraction and hydrolysis have been used as an alternative to traditional solvent extraction for many compounds in different matrices (1). Advantages include absence of chemical solvents, lower solvent costs, lower extraction times at moderate operational conditions, resulting in higher quality extracts. Furthermore, water is a solvent with very interesting properties in subcritical conditions. As temperature increases hydrogen bonds break, significantly decreasing the dielectric constant and the polarity, thus increasing solubility of more hydrophobic compounds. On the other hand water ionization increases (thus increasing H+ and OH- ions concentration), meaning that reactions catalysed by acids or bases, like biomass hydrolysis, are accelerated. [...], info:eu-repo/semantics/publishedVersion
- Published
- 2017
45. Novel polylactic acid (PLA)-based active packaging with incorporation of nanoparticles and its performance throughout shelf-life of fresh-cut fruit
- Author
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Rocha, João M., Lorite, Gabriela S., Miilumäki, Noora, Saavalainen, Paula, Selkälä, Tuula, Morales-Cid, Gabriel, Gonçalves, M. Pilar, Pongrácz, Eva, Toth, Geza, Rocha, Cristina M. R., and Universidade do Minho
- Subjects
Packaging ,Polylactic acid (PLA) ,Physicochemical/microbial properties ,Life cycle assessment (LCA) ,Ready-to-eat fresh-cut fruit - Abstract
This study aimed at developing innovative and environmentally friendly packages for fresh-cut fruits and at a better understanding their effect on physicochemical, mechanical and microbiological characteristics during shelf-life. Packages were developed under the scope of EU project SusFoFlex (7th framework programme) – thought to incorporate materials in final packaging formulations complying environmental and sustainability concerns and valorisation of agri-food by-products. Polylactic acid (PLA)-based active packaging formulations differed in nanoclays used and presence/absence of a surfactant. PLA-nanocomposite packaging performance was evaluated and compared with pristine-PLA and conventional polyethylene terephthalate (PET). Polyone was used as plasticizer in PLA packages. PET formulation did not include any nanoclay. Fresh-cut melon was selected as food model to assess PLA packaging formulations performance on quality changes taking place throughout 7-d storage under controlled conditions. Physicochemical and textural analysis over time encompassed weight loss, colour, visual appearance, pH, soluble solids and firmness, whereas microbial enumeration covered vegetative mesophilics and psychrotrofics, Gram- rods, nonsporing Gram+ rods and cocci, yeasts and moulds. Environmental impact of PLA-based packaging was evaluated via life cycle assessment (LCA) and compared with PET. Under limit storage conditions, all microbial groups exhibited maximum viable counts after 5-d. Pseudomonas aeruginosa and Escherichia coli were absent, and refrigeration proved to effectively reduce microbial activity. Overall inspection of dataset throughout storage, unfolded that nanoclays and surfactants in PLA formulations improved their performance, thus contributing to bring together the characteristics of both biopolymers (PLA and PET). Finally, LCA impact assessment indicated that PLA packaging with nanoclays had the highest environmental performance.
- Published
- 2016
46. On the correlation between physicochemical properties of agar and its performance as edible coating and film to enhance shelf-life of fresh fruit
- Author
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Rocha, João M., Sousa, Ana M. M., Santos, Joana, Alves, Rita, Gonçalves, Raquel, Oliveira, M. Beatriz, Gonçalves, M. Pilar, Rocha, Cristina M. R., and Universidade do Minho
- Subjects
Shelf-life of fresh fruit ,Gracilaria vermiculophylla ,Physicochemical and mechanical properties ,Agar-based films and coatings - Abstract
Agar is a biopolymer extensively used as gel and stabilizer. Depending on red seaweeds and agar extraction protocols, different physicochemical properties are attained. This research aimed at a better understanding the agar’s physicochemical properties effect on agar coatings performance to improve shelf-life of fresh fruits. Agar was extracted from red algae Gracilaria vermiculophylla in presence or absence of a pre-treatment with aqueous sodium hydroxide prior to extraction and purification. Agar extracts were characterized regarding molecular weight (Mw), sulphates, 3,6-anidrogalactoses (LA), gelation and melting point, extraction yield, gel strength, microstructure (via crio-SEM) and water content. Distinct agar-based coating and film formulations were prepared resorting to native and low sulphate and high anydrogalactose content-commercial agars. Glycerol and tween-80 were used as plasticizer and surfactant, respectively. Agar-based coatings were employed in fresh strawberries, which were further packed and stored at 10ºC, 80%RH and 25%van during 7-d, and shelf-life evaluated via several physicochemical, mechanical and nutritional parameters. Significant differences were observed in some properties, e.g. LA, sulphates, Mw, gel strength and microstructure – particularly in comparison to commercial agar. Coating data showed minor differences between native agar extracts and formulations but significant variations with commercial ones. Nevertheless, shelflife improvement was apparent in coated fruits when analysing colour and phenolics. Commercial agar led to slightly stronger films but native agar produced appropriated ones. This study confirms high correlations between extraction procedure and some properties of final agar which, in turn, have some key effects when used as edible coatings and films. Finally, potential use of G. vermiculophylla agar in coatings and films was established, contributing to the valorisation of environmental by-products.
- Published
- 2016
47. Dietary supplementation of heat-treated Gracilaria and Ulva seaweeds enhanced acute hypoxia tolerance in gilthead sea bream (Sparus aurata)
- Author
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European Commission, Fundação para a Ciência e a Tecnologia (Portugal), Ministerio de Economía y Competitividad (España), Magnoni, Leonardo [0000-0001-8449-6071], Martos-Sitcha, Juan Antonio [0000-0002-0151-7250], Calduch-Giner, Josep A. [0000-0003-3124-5986], Gonçalves, José Fernando [0000-0002-1418-7940], Rocha, Victoria G. [0000-0003-4687-0266], Abreu, Helena [0000-0002-7630-3185], Schrama, Johan W. [0000-0001-7156-8806], Magnoni, Leonardo, Martos-Sitcha, Juan Antonio, Queiroz, Augusto, Calduch-Giner, Josep A., Gonçalves, José Fernando, Rocha, Cristina M. R., Abreu, Helena, Schrama, Johan W., Ozório, R.O.A., Pérez-Sánchez, Jaume, European Commission, Fundação para a Ciência e a Tecnologia (Portugal), Ministerio de Economía y Competitividad (España), Magnoni, Leonardo [0000-0001-8449-6071], Martos-Sitcha, Juan Antonio [0000-0002-0151-7250], Calduch-Giner, Josep A. [0000-0003-3124-5986], Gonçalves, José Fernando [0000-0002-1418-7940], Rocha, Victoria G. [0000-0003-4687-0266], Abreu, Helena [0000-0002-7630-3185], Schrama, Johan W. [0000-0001-7156-8806], Magnoni, Leonardo, Martos-Sitcha, Juan Antonio, Queiroz, Augusto, Calduch-Giner, Josep A., Gonçalves, José Fernando, Rocha, Cristina M. R., Abreu, Helena, Schrama, Johan W., Ozório, R.O.A., and Pérez-Sánchez, Jaume
- Abstract
Intensive aquaculture practices involve rearing fish at high densities. In these conditions, fish may be exposed to suboptimal dissolved O levels with an increased formation of reactive O species (ROS) in tissues. Seaweeds (SW) contain biologically active substances with efficient antioxidant capacities. This study evaluated the effects of dietary supplementation of heat-treated SW (5% Gracilaria vermiculophylla or 5% Ulva lactuca) on stress bioindicators in sea bream subjected to a hypoxic challenge. 168 fish (104.5 g average weight) were distributed in 24 tanks, in which eight tanks were fed one of three experimental diets for 34 days: (i) a control diet without SW supplementation, (ii) a control diet supplemented with Ulva, or (iii) a control diet with Gracilaria. Thereafter, fish from 12 tanks (n=4 tanks/dietary treatment) were subjected to 24 h hypoxia (1.3 mg O l) and subsequent recovery normoxia (8.6 mg O l). Hypoxic fish showed an increase in hematocrit values regardless of dietary treatment. Dietary modulation of the O-carrying capacity was conspicuous during recovery, as fish fed SW supplemented diets displayed significantly higher haemoglobin concentration than fish fed the control diet. After the challenge, survival rates in both groups of fish fed SW were higher, which was consistent with a decrease in hepatic lipid peroxidation in these groups. Furthermore, the hepatic antioxidant enzyme activities were modulated differently by changes in environmental O condition, particularly in sea bream fed the Gracilaria diet. After being subjected to hypoxia, the gene expression of antioxidant enzymes and molecular chaperones in liver and heart were down regulated in sea bream fed SW diets. This study suggests that the antioxidant properties of heat-treated SW may have a protective role against oxidative stress. The nature of these compounds and possible mechanisms implied are currently being investigated.
- Published
- 2017
48. Dietary supplementation of heat-treatedGracilariaandUlvaseaweeds enhanced acute hypoxia tolerance in gilthead seabream (Sparus aurata)
- Author
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Magnoni, Leonardo J., primary, Martos-Sitcha, Juan António, additional, Queiroz, Augusto, additional, Calduch-Giner, Josep Alvar, additional, Gonçalves, José Fernando Magalhães, additional, Rocha, Cristina M. R., additional, Abreu, Helena T., additional, Schrama, Johan W., additional, Ozorio, Rodrigo O. A., additional, and Pérez-Sánchez, Jaume, additional
- Published
- 2017
- Full Text
- View/download PDF
49. Production of high added value products from eggshells
- Author
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Castro, Inês, Pinto, D. B., Maciel, José, Araújo, Ana, Vicente, A. A., Rocha, Cristina M. R., and Universidade do Minho
- Subjects
Eggshell ,Eggshell membranes ,Collagen ,Bioactive peptides - Abstract
There are an increasing number of food companies addressing sustainable-manufacturing practices as part of social responsibility reporting. The egg processing industry produces several tons of eggshell residues which are a major environmental problem and represent a significant cost for these industries (ca. 0.6 % of sales). The main goal of this work is to produce high added value products, namely collagen and bioactive peptides, using the remnant of collagen extraction from the eggshell membrane. The target market of the products obtained is the biomedical, pharmaceutical, cosmetics and food industry. The separation of the shell and the membrane was optimized and a chemical-physical process was performed having a yield of 9 % (w membrane/w shell). Times and yields for different separation methods were calculated and the economic viability of the process was studied. In order to optimize collagen extraction from the eggshell membranes several variables were studied (e.g. temperature, enzyme concentration and time). The collagen obtained was separated using saline precipitation steps and different collagen types were identified. The collagen types were visualized using SDS-electrophoresis and total quantification of collagen was made by hydroxyproline method. Moreover, the residue after collagen extraction was further digested with different enzymes, namely pancreatin, tripsin and lysin and the peptides profile was determined by HPLC-UV method. The results showed promising strategies for using this technology at industrial scale as an economic viable technology for producing high added value products from eggshells membranes. This technology will equally contribute to reducing the environmental problem associated with these residues.
- Published
- 2014
50. Diffusion of different molecular weight proteins through poly e-caprolactone films with encapsulated trypsin
- Author
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Martins, Artur J., Bourbon, A. I., Vicente, A. A., Silva, J. A. Lopes da, Rocha, Cristina M. R., and Universidade do Minho
- Published
- 2014
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