1. Treatment of biofilm infections on implants with low-frequency ultrasound and antibiotics.
- Author
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Carmen JC, Roeder BL, Nelson JL, Ogilvie RL, Robison RA, Schaalje GB, and Pitt WG
- Subjects
- Animals, Anti-Bacterial Agents administration & dosage, Cervical Vertebrae surgery, Disease Models, Animal, Escherichia coli drug effects, Female, Gentamicins administration & dosage, Injections, Subcutaneous, Intervertebral Disc surgery, Pseudomonas aeruginosa drug effects, Rabbits, Anti-Bacterial Agents pharmacology, Biofilms, Gentamicins pharmacology, Prostheses and Implants microbiology, Prosthesis-Related Infections prevention & control, Ultrasonics
- Abstract
Medical implants are sometimes colonized by biofilm-forming bacteria, which are very difficult to treat effectively. The combination of gentamicin and ultrasonic exposure for 24 hours was previously shown to reduce the viability of Escherichia coli biofilms in vivo. This article shows that such treatment for 48 hours reduced viable E coli bacteria to nearly undetectable levels. However, when Pseudomonas aeruginosa biofilms were implanted and treated for 24 and 48 hours, no significant ultrasonic-enhanced reduction of viable bacteria was observed. The difference in response of these 2 organisms is attributed to greater impermeability and stability of the outer membrane of P aeruginosa.
- Published
- 2005
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