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Interaction of immune cells with the systemic environment is necessary for the coordinated development and execution of immune responses. Monocyte-macrophage lineage cells reside at the junction of innate and adaptive immunity. Previously we reported that the sialyltransferase ST6GAL1 in the extracellular milieu modulates B cell development and IgG production, granulocyte production, and attenuates acute airway inflammation to bacterial challenge in mouse models. Here, we report that extracellular ST6GAL1 also elicits profound responses in monocyte-macrophage lineage cells. We show that recombinant ST6GAL1 adheres to subsets of thioglycolate-elicited inflammatory cells in the mouse peritoneum and to cultured human monocyte THP-1 cells. Exposure of the inflammatory cells to recombinant ST6GAL1 elicited wholesale changes in the gene expression profile of primary mouse myeloid cells; most notable was the striking up-regulation of monocyte-macrophage and monocyte-derived dendritic cell development pathway signature genes and transcription factors PU.1, NFκB and their target genes, driving increased monocyte-macrophage population and survival ex vivo. In the cultured human monocyte cells, the essential cell surface receptor of the monocyte-macrophage lineage, the M-CSF receptor (M-CSF-R, Csfr1) was a target of extracellular ST6GAL1 catalytic activity. Extracellular ST6GAL1 activated the M-CSF-R and initiated intracellular signaling events, namely, the nuclear translocation of NFκB subunit p65, and phosphorylation of ERK 1/2 and AKT. The findings implicate extracellular ST6GAL1 in monocyte development by a mechanism initiated at the cell surface and support an emerging paradigm of an extracellular glycan-modifying enzyme as a central regulator coordinating immune hematopoietic cell development and function.

Frequent blood donors who contribute multiple times annually are important for maintaining an adequate blood supply. However, repeated donations exacerbate iron deficiency, which can lead to pica, a condition characterised as repeated eating or chewing of a non-nutritious substance such as ice, clay and dirt. Understanding characteristics of frequent donors that are associated with increased risk for developing pica will help to identify them and prevent this adverse consequence of blood donation.Demographic, clinical, haematological, and biochemical factors associated with pica were investigated using univariable and multivariable logistic regression analysis in a cohort of 1693 high-intensity donors who gave nine or more units of whole blood in the preceding 2 years. Pica was classified by questionnaire responses as consuming at least 8 oz of ice daily and/or consumption of non-ice substances regardless of the amount and frequency.Pica was present in 1.5% of the high-intensity donors, and only occurred in those with ferritin50 ng/ml. Of 16 candidate variables, only haematocrit (OR = 0.835, p = 0.020) was independently associated with pica. Although severe iron deficiency was more prevalent in high-intensity donors, pica behaviours were less prevalent than in less frequent donors (2.2%).We have uncovered predictors of pica in high-intensity donors, which further emphasises the need to continue to implement iron replacement programs to reduce the prevalence of pica and maintain a robust pool of frequent donors.

Precision cancer medicine aims to classify tumors by site, histology, and molecular testing to determine an individualized profile of cancer alterations. Viruses are a major contributor to oncogenesis, causing 12% to 20% of all human cancers. Several viruses are causal of specific types of cancer, promoting dysregulation of signaling pathways and resulting in carcinogenesis. In addition, integration of viral DNA into the host (human) genome is a hallmark of some viral species. Tests for the presence of viral infection used in the clinical setting most often use quantitative PCR or immunohistochemical staining. Both approaches have limitations and need to be interpreted/scored appropriately. In some cases, results are not binary (virus present/absent), and it is unclear what to do with a weakly or partially positive result. In addition, viral testing of cancers is performed separately from tests to detect human genomic alterations and can thus be time-consuming and use limited valuable specimen. We present a hybrid-capture and massively parallel sequencing approach to detect viral infection that is integrated with targeted genomic analysis to provide a more complete tumor profile from a single sample.

Ammonia (NH3), dust and odor emissions from animal feeding operations (AFOs) can cause atmospheric pollution and disputes with neighbors. The objectives of this study were to: (1) re-design the ARS Air Scrubber to improve NH3 removal efficiency, (2) conduct full-scale testing of the scrubber under controlled conditions, (3) evaluate the efficacy of various acids for scrubbing NH3, and (4) determine the effects of air flow rate and NH3 concentration on scrubber performance. A full-scale prototype was constructed and a series of experiments were conducted under various conditions. Acid salts, such as aluminum sulfate (alum), sodium bisulfate, potassium bisulfate, ferric chloride and ferric sulfate were found to work as well as strong acids (hydrochloric, phosphoric and sulfuric) for capturing NH3. The efficiency of the scrubber for capturing NH3 decreased as the ventilation rate increased from over 90% at flow rates of 5,097 m3 hr-1 to around 55% at 16,141 m3 hr-1. However, the mass of N captured by the scrubber tripled as flow rates increased from 5,097 to 16,141 m3 hr-1. Similarly, there was a slight reduction in NH3 removal efficiency as inflow NH3 levels were increased from 10 µL L-1 to 75 µL L-1, whereas the mass of N captured increased from 25 g N hr-1 to around 200 g N hr-1. This technology could result in the capture of a significant amount of the N lost from AFOs, while simultaneously reducing emissions of dust and odors, which would improve the social and environmental sustainability of poultry and swine production.

Accumulation of phosphorus in soil is a major factor limiting the operational life of land application waste disposal systems. Moreover, for nutrient management purposes and evaluation of potential environmental problems it is necessary to understand the impact of manure application on soil phosphorus sorption characteristics. In this study laboratory experiments were conducted to investigate the impact of feedlot runoff effluent application on phosphorus sorption capacities, equilibrium phosphorus concentrations, and phosphorus buffering capacities of six Iowa soils. Soil samples were collected from vegetative treatment areas that had received feedlot runoff application for five years and from a paired grassed area that did not receive the effluent application. Subsamples of each soil were incubated with a series of twelve phosphorus solutions ranging in concentration from 0 to 200 mg P/L to determine the sorption characteristics of the soil. Sorption data were fitted to the Langmuir sorption model to determine the phosphorus equilibrium concentration, the phosphorus buffering capacity, and the maximum phosphorus sorption capacity of the soil. Sorption parameters of the vegetative treatment area and grassed area that didn’t receive the feedlot runoff were then compared to evaluate the impact effluent application had on soil phosphorus sorption properties. Results indicated that vegetative treatment areas generally had elevated phosphorus equilibrium concentrations in relation to the grassed area that didn’t receive the effluent application, indicating an elevated risk of loss of dissolved phosphorus. In most cases the ability of the soil to sorb phosphorus was significantly increased as was the remaining phosphorus sorption capacity of the soil. These results indicate that vegetative treatment area life could be greatly extended due to soil property modifications that occur as a result of system use.

Aerial emissions from livestock production continue to be an area of attention and concern for both the potential health and environmental impacts. However, information of gaseous, especially greenhouse gas (GHG), emissions for swine breeding/gestation and farrowing production systems is limited. The purpose of this study was to quantify ammonia (NH3), carbon dioxide (CO2), nitrous oxide (N2O), and methane (CH4) concentrations and emissions from a modern breeding-gestation-farrowing system located in central Iowa, USA. A 4300-sow farm was selected for the extensive field monitoring which employed a Mobile Air Emission Monitoring Unit equipped with state-of-the-art gas analyzers and a data acquisition system. The monitored portion of the farm facility consisted of a deep-pit breeding/early gestation (B/EG) barn (1800 head), a deep-pit late gestation (LG) barn (1800 head), and two shallow-pit (pull-plug) farrowing rooms (40 head per room). A dynamic flux chamber was used to monitor gaseous emissions from the external manure storage for the farrowing rooms. Data were collected for 29 consecutive months (January 2011 through June 2013). Daily indoor NH3, CO2, N2O, and CH4 concentrations (ppm, mean ± SD) were 12.0 (±7.6), 1594 (±797), 0.31 (±0.11), and 28.5 (±9.8), respectively, in the breeding/gestation barns; and 9.7 (±4.1), 1536 (±701), 0.30 (±0.10), and 78.3 (±37), respectively, in the farrowing rooms. Daily emissions per animal unit (AU, 500 kg live weight) were 35.1 g NH3, 7.46 kg CO2, 0.17 g N2O, and 263.4 g CH4 for sows in the B/EG barn; and 28.2 g NH3, 6.50 kg CO2, 0.12 g N2O, and 201.3 g CH4 for sows in the LG barn. The average daily emissions per AU (sow and piglets) of the farrowing rooms during the lactation period (birth to weaning) were: 59.7 g NH3, 16.4 kg CO2, 0.73 g N2O, and 107 g CH4. For the monitored period, the external manure storage had the following average daily emission per m2 surface area: 1.26 g NH3, 137 g CO2, and 94.8 g CH4, which was equivalent to daily emissions per AU in the farrowing rooms of 12.2 g NH3, 1.055 kg CO2, and 867 g CH4. Average daily emissions per AU for the total operation (including house-level and manure storage emissions) were 38.5 g NH3, 8.73 kg CO2 (including 7.3 kg from animal respiration), 0.25 g N2O, and 301 g CH4.

Increased environmental awareness has prompted the need for improved feedlot runoff control. Vegetative treatment systems (VTSs) provide a cost-effective option that may enhance environmental security by protecting water quality. Vegetative treatment systems are typically designed on the basis of hydraulic performance, which may result in excess application of some nutrients, specifically nitrogen and phosphorus. Groundwater quality monitoring is required to determine the effect, if any, that VTSs have on groundwater. Shallow groundwater (2 to 10 m) quality beneath six VTSs in Iowa was monitored over a four-year period. Monitoring wells were located upgradient, within, and downgradient of the VTSs. Groundwater samples were collected on a monthly basis and analyzed for ammoniacal nitrogen, chloride, nitrate-nitrogen, and fecal coliforms. A trend analysis was conducted to evaluate groundwater response patterns to VTS construction and use. In general, monitoring wells located within and downgradient of the VTS showed increasing trends in chloride and decreasing trends in nitrate concentrations. No trends for fecal coliforms or ammoniacal nitrogen were seen. Statistical analysis was performed to test for concentration differences between upgradient, within, and downgradient monitoring wells. In general, no differences in ammoniacal nitrogen concentration were seen. Fecal coliform concentrations were generally highest at the monitoring well within the VTS, but no difference was found between upgradient and downgradient concentrations. Chloride concentrations were generally significantly higher within and downgradient of the VTS when compared to the upgradient well; nitrate concentrations were generally significantly lower within and downgradient of the VTA than upgradient.

Beef feedlots of all sizes are looking for more cost-effective solutions for managing feedlot runoff. Vegetative treatment systems are one potential option, but require performance evaluation for use on concentrated animal feeding operations. The performance of six vegetative treatment systems on open beef feedlots throughout Iowa was monitored from 2006 through 2009. These feedlots had interim, National Pollution Discharge Elimination System permits that allowed the use of vegetative treatment systems to control and treat runoff from the open feedlots. This manuscript focuses on making within site comparisons, i.e., from year-to-year and component-to-component within a site, to evaluate how management changes and system modifications altered performance. The effectiveness, in terms of effluent concentration reductions, of each system was evaluated; nutrient concentration reductions typically ranged from 60 to 99% during treatment in the vegetative components of the vegetative treatment systems. Monitoring results showed a consistent improvement in system performance during the four years of study. Much of this improvement can be attributed to improved management techniques and system modifications that addressed key performance issues. Specifically, active control of the solid settling basin outlet improved solids retention and allowed the producers to match effluent application rates to the infiltration rate of the vegetative treatment area, reducing the occurrence of effluent release. Additional improvements resulted from system maturation, increased operator experience, and the addition of earthen flow spreaders within the vegetative treatment area to slow flow and provide increased effluent storage within the treatment area, and switching to active management of settling basin effluent release.

Beef feedlots of all sizes are looking for cost-effective solutions to manage feedlot runoff. Vegetative treatment systems (VTSs) are a potential option. VTSs consist of a solids settling structure followed by additional treatment components, such as vegetative infiltration basins (VIBs) and/or vegetative treatment areas (VTAs) that use soil and vegetation to treat nutrients in the applied runoff. Investigations have shown that VTSs can provide a cost-effective means of controlling feedlot runoff; however, their sustainability and life expectancy have not yet been determined. Thus, the objective of this work is to evaluate, based on the VTA’s ability to sorb and utilize phosphorus, the expected phosphorus sink life of VTSs on beef feedlots in Iowa. In doing so, we evaluated three things: (1) phosphorus removal with vegetation harvest, (2) the extent of vertical redistribution of phosphorus in the soil profile, and (3) if a mass balance approach was capable of predicting changes in soil test phosphorus. Vegetation harvest removed 6% to 16% of the applied phosphorus, and a P mass balance did an adequate job of predicting the significant increases in soil P test concentrations. Deep soil cores (1.2 m) showed that phosphorus accumulation tended to be limited to the top 0.3 m but that vertical migration was increasing. Based on this success, we proposed a P mass balance and soil sorption model to project VTA life expectancy and evaluated the sensitivity of the estimated life to different design and management alternatives. The sensitivity analysis showed that phosphorus sorption capacity and loading rate were important, but the critical depth of the soil that can be saturated has the largest impact on VTA life.

Quantifying non-methane hydrocarbons (NMHC) from animal feeding operations (AFOs) is challenging due to the broad spectrum of compounds and the polar nature of the most abundant compounds. The purpose of this study was to determine the performance of commercial NMHC analyzers for measuring volatile organic compounds (VOCs) commonly emitted from AFOs. Three different NMHC analyzers were tested for response to laboratory generated VOCs, and two were tested in the field at a commercial poultry facility. The NMHC analyzers tested included gas chromatography/flame ionization detector (GC/FID), photoacoustic infrared (PA-IR) and photoionization detector (PID). The GC/FID NHHC analyzer was linear in response to nonpolar compounds, but detector response to polar oxygenated compounds were lower than expected due to poor peak shape on the column. The PA-IR NMHC instrument responded well to the calibration standard (propane), methanol, and acetone, but it performed poorly with larger alcohols and ketones and acetonitrile. The PA-IR response varied between compounds in similar compound classes. The PID responded poorly to many of the most abundant VOCs at AFOs, and it underreported alcohols by70%. In the field monitoring study, total NMHC concentrations were calculated from sum total of VOC determined using EPA Methods TO-15 and TO-17 with GC-MS compared to results from NMHC analyzers. NMHC GC/FID values were greater than the values calculated from the individual compound measurements. This indicated the presence of small hydrocarbons not measured with TO-15 or TO-17 such as propane. The PA-IR response was variable, but it was always lower than the GC/FID response. Results suggest that improved approaches are needed to accurately determine the VOC profile and NMHC emission rates from AFOs.

Feed additives can change the microbiological environment of the animal digestive track, nutrient composition of feces, and its gaseous emissions. This 2-yr field study involving commercial laying-hen houses in central Iowa was conducted to assess the effects of feeding diets containing EcoCal and corn-dried distillers grain with solubles (DDGS) on ammonia (NH3), hydrogen sulfide (H2S), and greenhouse gas (CO2, CH4, and N2O) emissions. Three high-rise layer houses (256,600 W-36 hens per house) received standard industry diet (Control), a diet containing 7% EcoCal (EcoCal) or a diet containing 10% DDGS (DDGS). Gaseous emissions were continuously monitored during the period of December 2007 to December 2009, covering the full production cycle. The 24-month test results revealed that mean NH3 emission rates were 0.58 +/- 0.05, 0.82 +/- 0.04, and 0.96 +/- 0.05 g/hen/day for the EcoCal, DDGS, and Control diet, respectively. Namely, compared to the Control diet, the EcoCal and DDGS diets reduced NH3 emission by an average of 39.2% and 14.3%, respectively. The concurrent H2S emission rates were 5.39 +/- 0.46, 1.91 +/- 0.13, and 1.79 +/- 0.16 mg/ hen/day for the EcoCal, DDGS, and Control diet, respectively. CO2 emission rates were similar for the three diets, 87.3 +/- 1.37, 87.4 +/- 1.26, and 89.6 +/- 1.6 g/hen/day for EcoCal, DDGS, and Control, respectively (P = 0.45). The DDGS and EcoCal houses tended to emit less CH4 than the Control house (0.16 and 0.12 vs. 0.20 g/hen/day) during the monitored summer season. The efficacy of NH3 emission reduction by the EcoCal diet decreased with increasing outside temperature, varying from 72.2% in February 2009 to -7.10% in September 2008. Manure of the EcoCal diet contained 68% higher ammonia nitrogen (NH3-N) and 4.7 times higher sulfur content than that of the Control diet. Manure pH values were 8.0, 8.9, and 9.3 for EcoCal, DDGS, and Control diets, respectively. This extensive field study verifies that dietary manipulation provides a viable means to reduce NH3 emissions from modern laying-hen houses.

Treating animal wastes through anaerobic digestion (AD) yields methane-rich biogas that can be used for power generation or heating, and a nutrient-rich digestate that can be land-applied as fertilizer. Furthermore, AD reduces odors from stored and land-applied manures. Despite these benefits, AD deployment rates in the U.S. are only 5% for dairy farms identified as suitable for AD by the U.S. Environmental Protection Agency. The objective of this study was to analyze the economic and technical limitations of farm-scale anaerobic digesters using a simple model permitting insight into the fundamental constraints on the technology. A model was developed to determine the cost of methane produced via AD based on operation size. Dairy plug-flow systems were modeled because of their well-documented economic performance, and model validation used data from AgSTAR's FarmWare program. The analysis shows that farm size is critical to make digestion-derived methane cost-competitive with natural gas. At low herd sizes (

Particulate matter (PM) associated with animal feeding operations is a concern for the occupants and the surrounding community. Baseline measurements of PM concentration and emission rate are the first step toward assessing the magnitude of concentrations and emissions and evaluating effectiveness of dust control strategies. This study presents the results of PM measurements at a high-rise layer house (approx. 250,000 hens) in central Iowa using tapered element oscillating microbalance (TEOM) equipment. Daily average concentrations of PM10 and PM2.5 over a 17-month measurement period were 393 (257 SD) and 44 (36 SD) g m -3 , respectively. Daily average PM10 and PM2.5 emission rates during the same monitoring period were, respectively, 26.1 (15.8 SD) and 3.6 ( 3.7 SD) mg bird -1 d -1 , or 8.16 (4.94) and 1.13 (1.16) g AU -1 d -1 (AU = animal unit = 500 kg body weight). PM emission rate was positively related to ventilation rate

Beef feedlot runoff is a potential environmental contaminant. As such, it should be managed properly to preserve water quality. Primary treatment of feedlot runoff often relies on sedimentation techniques; thus, accurate knowledge of feedlot runoff physical properties is required. This study characterized the physical and chemical properties of runoff effluent from earthen and concrete beef feedlots in Iowa with the objective of providing the necessary information to improve solid settling basin design and performance. Results, although not statistically significant (p = 0.11), indicated that solids in runoff from concrete lots tended to settle more slowly than solids from earthen lots. Particle size distribution and particle density measurements indicated that the poorer settleability of concrete lot runoff was primarily caused by lower particle densities: 1.47 ±0.17 g cm-3 (average ± SD) for concrete lots as compared to 1.89 ±0.11 g cm-3 for earthen lots. Runoff composition was analyzed before and after settling to relate nutrient reduction to solids removal. Results indicated an average of 41 g total Kjeldahl nitrogen per kg total solids and 16 g total phosphorus per kg total solids were removed during settling.

There has been an increasing interest in manure anaerobic digestion ; however, economic constraints are still one of the limits to widespread use of the technology in the United States. Co-digestion of manure with other feedstocks has been noted as a way to increase the economic feasibility of animal feeding operation anaerobic digesters via increased energy production potential. A wide variety of materials have been proposed as co-digestion materials, and additional substrates will continue to receive consideration. Biochemical methane potential assays (BMPs) have been reported to provide a "first-cut" evaluation of potential substrates. This article provides specific details about the BMP assay process used by the Agricultural Waste Management Laboratory (AWML) at Iowa State University (ISU) on agricultural materials and by-products, including the assay method and utilization of the results. Additionally, BMP results from 31 samples assayed in the ISU AWML as broader anaerobic digestion research or as service to the industry have been included. Results showed that the high solid content and non-homogeneity of agricultural materials and by-products can increase variability in assay results. The method utilized here helped limit the effects by utilizing volatile solids concentrations instead of chemical oxygen demand to initiate the BMP assays and to normalize the results. The coefficient of variation for the assays performed in triplicate ranged from1.6% to 33% in which the majority was less than 15%. For five of the substrate types analyzed (beef manure, dairy manure, cheese when lactate permeate, food processing marinate, and enzyme process by-product), multiple samples were assayed from different sources. The sample standard deviations indicated that methane production potential could be affected by material source and that BMP assays reported here should only be used as an estimate when considering which types of materials to assay.

Emissions of gaseous compounds and particulate matter are the product of the pollutant concentrations and air exhausted from the fans of mechanically ventilated animal confinements. Direct methods of monitoring exhaust fan operation (mercury tilt, limit/ whisker, and vibration switches) have been reported to have limitations due to mechanical failure and/or the effect of dust, wind, and moisture. The objective of this study was to find a reliable method of monitoring fan operation status. This article describes the development, lab testing, and field use of a fan monitoring system based on an induction-operated current switch (ICS). The ICS is unaffected by the environment and can provide direct measurement of real-time fan operational status by sensing the AC current drawn by the fan motor. A laboratory test of the ICS was performed to simulate a fan off/on duty cycle for a two-year field emissions monitoring study; no ICS failure was recorded. Three studies led by Iowa State University (Southeastern Broiler Gaseous and Particulate Matter Emission, Determining Ammonia and Particulate Matter Emissions from a Midwest Turkey Grow-Out Building, and Feeding DDGS and Other Altered Diets to Egg Laying Hens to Demonstrate Economically Viable Reductions in Ammonia Emissions) used a total of 28, 12, and 50 ICS systems for 24, 16, and 27 months, respectively, without a non-user error related failure. At a unit cost as low as $21.45 this method offers a reliable, accurate, and economical way of measuring the real-time operational status of ventilation fans – a critical component of any air emissions monitoring in a mechanically ventilated confinement.

Design and construction of full-scale anaerobic digesters that co-digest manure with other substrates, such as food processing wastes, is challenging because of the large number of potential mixtures that can be fed to the digester. In this work we examine the relationship between results from bench-scale methods such as biochemical methane potential assays (BMPs) and sub pilot-scale reactors. The baseline feedstock for this study was beef manure from concrete feedlot pens (open and covered) in eastern Iowa. Additional co-digestion substrates tested were short-fiber cardboard, corn processing wastewater, enzyme processing wastewater and lagoon liquid. Substrates were characterized for total solids (TS), volatile solids (VS), chemical oxygen demand (COD), pH, alkalinity, and ammonia, after which BMPs were conducted on all substrates. Based on the BMP and anaerobic toxicity assay (ATA) results, a mixture was created and evaluated using BMPs and tested in 100-L sub pilot-scale reactors. This study showed that results from BMPs of feedstock co-digestion mixtures accurately estimated the range of methane produced from three 100-L, plug flow reactors.

Vegetative treatment systems (VTSs) provide an alternative to containment basin systems for beef feedlot runoff control. Beef producers in the Midwestern United States have shown an increasing interest in using VTSs as a perceived lower cost option to containment basin systems. This article reports the actual construction costs associated with 23 VTSs (nine on permitted Concentrated Animal Feeding Operations (CAFOs) and 14 on non-permitted Animal Feeding Operations (AFOs)) and four containment basins located throughout Iowa, Minnesota, South Dakota, and Nebraska. The VTS construction costs are reported on a per head space basis in 2009 adjusted dollars for each system. Cost comparisons are presented for CAFO and AFO facilities, and by system type. Additionally, estimated construction cost comparisons between open feedlots with VTS systems, open feedlots with containment basins, monoslope barns and hoop structures for beef production systems are provided. Results from the cost comparison indicate the average construction cost in 2009 dollars for an AFO or CAFO is $655 per head space for animals housed in a monoslope barn with a concrete floor and $395 per head space for animals housed in a hoop structure. For AFOs and CAFOs, the average cost of an earthen lot with a containment basin costs is $361 per head space, while the average cost of an earthen lot implemented with a VTS is $283 per head space. If only the feedlot runoff control system is considered, VTSs designed for CAFO facilities are less expensive on average to construct ($85 per head space on average) than traditional containment basins ($136 per head space on average). Similarly for AFO feedlot runoff control systems, a VTS was less expensive to build on average ($79 per head space on average.) than a containment basin on a similar facility ($205 per head space). The data indicated on average the least expensive VTS for an AFO is a gravity VTS ($54 per head space average.) followed by a sprinkler VTS ($94 per head space average.) and a pump and gravity VTS ($101 per head space average.). Statistical analysis showed no significant difference between the VTS construction cost per head space of cattle for an AFO compared to a CAFO (p=0.07, alpha=0.05) while there was a statistical difference between system type (p=0.02, alpha=0.05).

While there has been increasing interest in manure anaerobic digesters during the past decade, economic constraints remain a limitation to widespread use of the technology. Literature has suggested that co-digestion with other available organic co-substrates could increase the economic viability of manure anaerobic digesters by increasing methane production potential. However, co-substrates must be carefully selected to avoid adding complexities to the manure digestion process. Biochemical methane potential (BMP) assays have been reported to provide a good initial evaluation of potential co-substrates, but they are performed under conditions optimized for the microbes, and the co-substrate is assayed under diluted conditions. Anaerobic toxicity assays (ATAs) provide additional information that could be utilized with BMP results to assist with co-substrate selection. An ATA evaluates a substrate's ability to inhibit methane production and thus determine its potential toxicity. This article provides specific details about the ATA process used by the Agricultural Waste Management Laboratory at Iowa State University on potential digester materials, including the assay method and utilization of the results. The two potential digester materials presented in this article provide examples of a toxic and a non-toxic material. Specifically, normalized BMP results indicated that the industry process by-product produced 60 mL CH4/g VS, and that it was not toxic at material inclusion rates of up to 49%. Conversely, the enzyme process by-product produced normalized BMP results yielding 284 mL CH4/g VS, but based on ATA results was highly toxic at all inclusion rates. These results clearly indicate the need to thoroughly evaluate co-digestion co-substrates.

Volatile organic compounds (VOCs) emitted from poultry production are leading source of air quality problems. However, little is known about the speciation and levels of VOCs from poultry production. The objective of this study was the speciation of VOCs from a poultry facility using evacuated canisters and sorbent tubes. Samples were taken during active poultry production cycle and between production cycles. Levels of VOCs were highest in areas with birds and the compounds in those areas had a higher percentage of polar compounds (89%) compared to aliphatic hydrocarbons (2.2%). In areas without birds, levels of VOCs were 1/3 those with birds present and compounds had a higher total percentage of aliphatic hydrocarbons (25%). Of the VOCs quantified in this study, no single sampling method was capable of quantifying more than 55% of compounds and in several sections of the building each sampling method quantified less than 50% of the quantifiable VOCs. Key classes of chemicals quantified using evacuated canisters included both alcohols and ketones, while sorbent tube samples included volatile fatty acids and ketones. The top five compounds made up close to 70% of VOCs and included: 1) acetic acid (830.1 μg m −3 ); 2) 2,3-butanedione (680.6 μg m −3 ); 3) methanol (195.8 μg m −3 ); 4) acetone (104.6 μg m −3 ); and 5) ethanol (101.9 μg m −3 ). Location variations for top five compounds averaged 49.5% in each section of the building and averaged 87% for the entire building.

This article compares results from the Iowa State University Effluent Limitations Guidelines (ISU-ELG) model to results obtained using the Soil-Plant-Air-Water (SPAW) model to simulate feedlot runoff containment basin overflow volume. The objective was to verify that the ISU-ELG model was providing a reasonable prediction of basin overflow. The ISU-ELG model uses a set of guidelines to determine if land application is acceptable, whereas the SPAW model uses a soil moisture criterion. The criterion for determining if a particular day was suitable for land application of basin effluent was investigated to determine the effect on basin overflow volumes. The results show that the ISU-ELG model overpredicted the percentage of feedlot runoff controlled in comparison to the SPAW model at all five locations investigated. For wetter areas in Iowa, the number of drying days had a large effect on basin overflow volumes, whereas for the drier northwest region of Iowa, this effect was limited. Possible methods of improving the ISU-ELG model predictions include adding a soil moisture accounting function to model moisture levels in the land application area or calibrating the number of drying days required before land application can commence. Alternatively, the SPAW model can be used, but this requires additional user inputs and increases complexity in modeling the runoff control system.

This article presents the biochemical methane potential (BMP) results from the anaerobic digestion (AD) of switchgrass. Triplicate BMP assays were performed on: untreated switchgrass, aqueous ammonia soaking (AAS) pretreated switchgrass (soaked in 29.5% reagent-grade aqueous ammonia at 5 L kg-1 switchgrass for 5 d), and AAS-pretreated switchgrass plus cellulytic enzymes at 12.5, 25, 62.5, and 125 filter paper units (FPU) enzyme g-1 volatile solids (VS). Biogas production and biogas methane content were measured daily in all treatments for 21 d. Both biogas and corrected methane production varied significantly among treatments, especially during the first 7 d of the BMP period. Total methane production at 21 d was corrected for enzyme degradation, and methane yields ranged from 0.15 to 0.36 m3 CH4 kg-1 VS. We compared the corrected energy yield of biogas from switchgrass to prior reports of the energy yield of ethanol from switchgrass via simultaneous saccharification and fermentation (SSF). The AD of AAS-pretreated switchgrass at the highest enzyme loading rates resulted in a 120% increase in energy extracted as compared to AAS-pretreated switchgrass converted to ethanol via SSF. Overall, the addition of enzymes to AAS-pretreated switchgrass greatly accelerated the rate of methane production over the untreated switchgrass and AAS-pretreated switchgrass without enzymes. However, the process economics are not clear, and additional work is needed to determine whether pretreating switchgrass with aqueous ammonia and/or enzymes before AD is economically advantageous.

This article addresses the biochemical methane potential (BMP) production from anaerobic digestion of corn-ethanol coproducts including dried distiller grain with solubles (DDGS), distiller's wet grains (DWG), thin stillage, and condensed distiller's solubles (CDS) as well as evaluating the effects of ultrasonic pretreatment on methane production from these feedstocks. Ultrasonic pretreatment was applied with three amplitude settings of 33% (52.8 µmpp), 66% (105.6 µmpp), and 100% (160 µmpp) as well as five time settings (10, 20, 30, 40, and 50 s) to each of the four coproducts prior to conducting benchtop BMP trials. Ultrasonic pretreatment reduced mean particle size of DDGS and DWG by 45% and 43%, respectively. Without ultrasound pretreatment, CDS had the highest methane production potential (407 mL g-1 VS added) compared to the other coproducts. Ultrasonic pretreatment of DWG co-products (DDGS and DWG) resulted in greater increases in methane production than on liquid coproducts (CDS and thin stillage). Methane yields were increased by 25% and 12% for the ultrasound pretreated DDGS and DWG, respectively, compared with untreated samples. An energy balance for the DWG, thin stillage, and CDS coproducts indicated that ultrasonic pretreatment required more energy than was generated by the process in terms of additional biogas production. However, an energy balance for ultrasonic pretreatment of DDGS provided 70% more energy than was required to operate the ultrasonic unit.

This article addresses the effect of ultrasonication as a pretreatment to anaerobic digestion of four types of animal manure, including swine slurry, beef feedlot manure, dairy manure slurry, and separated dairy manure effluent. The effect of ultrasonication on soluble chemical oxygen demand (SCOD) and biochemical methane potential (BMP) were determined, and the energy efficiency of ultrasonic pretreatment was evaluated. Ultrasonic pretreatment was applied at two amplitudes (80 and 160 µmpp) and at two time settings (15 and 30 s) to each of the four manure types. The SCOD of each manure sample was determined before and after ultrasonic pretreatment. In addition, BMP trials were run on each waste with and without ultrasonic pretreatment. As part of the BMP, biogas production was measured and analyzed for methane content and cumulative methane production. Ultrasonic pretreatment of swine slurry, beef feedlot manure, dairy manure slurry, and separated dairy manure effluent increased the average SCOD up to 23%, 92%, 59%, and 33%, respectively, and the average methane yield up to 56%, 43%, 62%, and 20%, respectively. Increasing the ultrasonic amplitude and treatment time resulted in an increase in manure SCOD and methane production; the greatest methane production was obtained using the ultrasonic pretreatment at the highest power and longest treatment time. The observed greatest methane production from swine slurry, beef feedlot manure, dairy manure slurry, and separated dairy manure effluent were 394, 230, 226, and 340 mL CH4 g-1 VS, respectively. In contrast, the greatest energy efficiency was obtained with the lowest ultrasonic amplitude combined with the shortest treatment time.

The objective of this research was to test a pilot-scale air sparged tank reactor (ASTR) and the ASTR in combination with a hydrocyclone (called the pilot-scale ASTR-hydrocyclone system) on two swine manure slurries for struvite-based (MgNH4PO4-6H2O) phosphorus removal and recovery. The pilot-scale ASTR system operated at flow rates of 80 to 115 L/min and was based on the bench-scale design from Shepherd et al. (2007). The ASTR effluent was processed using a hydrocyclone separator for struvite separation and total phosphorus (TP) recovery. The pilot-scale ASTR-hydrocyclone system provided a 92% reduction of dissolved reactive phosphorus (DRP) in manure slurry from a swine finishing facility concrete storage tank and a 91% reduction of DRP in manure slurry collected from a swine finishing facility deep-pit under floor collection system. The pilot-scale ASTR-hydrocyclone system removed 18% of TP in swine manure from a concrete storage tank and 9% to 14% of TP in swine manure slurry from a deep-pit under floor collection system. The low TP recovery was attributed to the hydrocyclones inability to provide effective struvite separation as operated. Full-scale economics and implementation of the tested struvite-based phosphorus removal is discussed. A case study of a typical Iowa deep-pit swine production facility (10,000 head/year) indicated that the annual cost of struvite-based phosphorus removal using this system would be approximately $8.88/finished pig or $0.035/L manure slurry treated ($ 0.134/gal). This cost often exceeds producer's profit margins; this indicates that struvite-based phosphorus removal using this ASTR-hydrocyclone system in swine finisher manure slurries is not currently economically viable.

Forced precipitation of struvite (MgNH4PO4 . 6H2O) can reduce dissolved reactive phosphorus (DRP) in swine manure slurries. Optimization of this process requires that the swine manure slurry pH be increased, that magnesium be added, and that sufficient reaction time be allowed for struvite precipitation. To gather data that could be used for a full-scale continuous-flow struvite precipitation reactor, a bench-scale (14-L) continuous flow reactor was designed, constructed, and tested. The bench-scale reactor used air sparging for both pH adjustment and mixing, used a peristaltic pump to continuously inject magnesium chloride (MgCl2 . 6H2O), and was operated at a 10-min hydraulic retention time. The bench-scale system provided a 95% reduction of DRP in swine manure slurry collected from a concrete storage tank with a permeable cover, and a 78% reduction of DRP in swine manure slurry collected from a shallow under floor pit collection system. A bench-scale up-flow clarifier was designed, constructed, and tested for continuous flow separation of the precipitated struvite in order to provide total phosphorus (TP) removal. The up-flow clarifier was unable to continuously settle struvite particles formed in the bench-scale reactor and provided no significant TP removal through the system. The implication of this work for full-scale systems is discussed.

Analysis of the propagation of measurement error into a computed quantity such as building aerial emissions provides insight into which measurements are most critical and which would have the most impact on the computed quantity if improved. An analysis of different instrument measurements, sampling periods, and sites together comprise an objective means of determining optimal sampling strategies for measurements used to compute aerial emissions from livestock facilities. This article describes the uncertainty analysis for a measurement system used in emissions research, and how it can lead to improvements in measurement system design and implementation to obtain estimates of uncertainty in emissions. The system analyzed was used in a broiler house emission monitoring project that was part of the U.S. EPA Air Consent Agreement. The project required U.S. EPA category I Quality Assurance Project Plan (QAPP) Data Quality Objectives (DQO), which were developed from this uncertainty analysis. Results of the uncertainty analysis suggest that the combined standard uncertainty in ammonia emission from broiler houses in the study was typically less than 6%; it increased with uncertainty in ventilation rate, but decreased as ventilation rate and number of fans running increased. The combined standard uncertainty was quantified for normal measurement conditions (Case 1) and for conditions in which the instrumentation was at the calibration threshold (Case 2). A key conclusion was that, for the measurement system employed in this project, uncertainty in the measurements associated with ventilation rate are the major contributors to emissions rate uncertainty (ranging from 78% to 98.9% of combined standard emission uncertainty).

Swine production facilities today are larger in size and are concentrated in smaller geographical areas than they were two decades ago. Additionally, there is an increasing trend to base manure land application rates on crop phosphorus requirements rather than crop nitrogen requirements alone. These factors have resulted in a need to find a feasible method for removing phosphorus from swine waste prior to land application. Economically concentrating and transporting excess phosphorus to phosphorus deficient cropping areas would benefit the current production scheme. Forced precipitation of phosphorus as struvite (MgNH4PO46H2O) is an option for phosphorus concentration and removal. Economic feasibility of phosphorus precipitation improves when reactive phosphorus represents a high percentage of the total phosphorus in slurry, and when a limited amount of chemical amendment is required for the precipitation reaction. In this study, anaerobic digestion of swine slurry increased reactive phosphorus (PO43-) and magnesium ion (Mg2+) in solution by 26% and 254%, respectively. Additionally, during phosphorus precipitation experiments, PO43- removal and reduction efficiencies were greatest in the tests performed with anaerobically digested swine slurry.

Accurate ventilation rate (VR) data are essential to maximizing the quality of aerial emission measurements. The Fan Assessment Numeration System (FANS) has been widely used by U.S. researchers in measuring aerial emissions from mechanically ventilated livestock and poultry facilities. The FANS device is used to measure airflow rates of ventilation fans in situ, thereby developing fan performance curves under field conditions. The FANS device was originally intended to be placed upstream of the fan to be calibrated. However, certain field situations make it impractical to apply the FANS device as such. This study was conducted to assess use of the FANS device downstream of a ventilation fan, with the gaps between the FANS device and the discharge cone of the exhaust fan sealed using a non-permeable fabric. Nine exhaust fans (1.22 or 1.32 m diameter) in laying hen and turkey houses were tested with the FANS device placed upstream and downstream for a building static pressure range of 10 to 40 Pa. The results revealed that downstream placement of the FANS device yielded 0.6% ±0.4% to 4.0% ±0.9 % (mean ±SE) higher but not significantly different (P > 0.28) VR values as compared to upstream placement for the exhaust fans tested. This magnitude of discrepancy is considered acceptable for in situ measurement of fan performance.

The goal of this project was to develop a system that identified magnesium demand for struvite formation by monitoring conductivity changes during continuous injection of magnesium chloride in several swine manure slurries. The conductivity of six manure slurries was monitored to identify the response due to magnesium chloride injection (MgCl2) and struvite precipitation. Struvite precipitation is a technically feasible treatment method for phosphorus removal and recovery from manure slurries (Burns et al., 2003; Bowers and Westerman, 2005a). Swine manure slurries often require the addition of magnesium (Mg 2+ ) to force struvite precipitation. The quantity of Mg 2+ required for maximized phosphorus removal can be determined through laboratory tests. Optimized struvite precipitation in a field setting requires a real-time method to determine Mg 2+ addition rates during a land application event. This article discusses the requirements of an automated control system which monitors and controls the injection of Mg 2+ to force struvite precipitation, accounting for real-time variations of magnesium demand. Theoretical predictions and pure solution tests provided information capable of determining the magnesium demand for struvite precipitations. After testing six different manures in triplicate, the conductivity responses did not follow theoretical predictions and failed to provide any indication of optimum magnesium injection rates for phosphorus removal.

Ventilation rate (VR) is one of the two key elements for quantifying aerial emissions from animal production facilities. Direct, continuous measurement of building VR can be challenging and impractical under certain circumstances, e.g., naturally ventilated animal housing or a large number of ventilation fans in the building. This study examined the suitability of estimating VR of broiler houses with built-up litter (mixture of manure and bedding), when supplemental heating was not in use, through either carbon dioxide (CO 2 ) balance or the relationship of VR to CO 2 concentration difference between exhaust and inlet air. The reference VR was based on direct measurement by continuously monitoring operation of the in-situ calibrated exhaust fans. The comparative analysis of the direct method vs. each indirect method was conducted for a measurement integration time (MIT) of 10, 30, 60, or 120 min. The analyses revealed that MIT of 30 min or greater resulted in non-significant differences in VR between the indirect and direct methods. The broiler building VR (m 3 s -1 ) may be related to the exhaust-inlet CO 2 concentration difference (ΔCO 2 , ppm) as VR (±3.0) = 4456 (±41) ΔCO 2 -0.786 (±0.019) at 30 min MIT. The VR may also be determined by the CO 2 balance method (including litter CO 2 generation) with a correction factor of 0.97 at MIT of 30 to 120 min. If litter CO 2 generation is omitted from the total building CO 2 production, the actual VR may be estimated by applying a correction factor of 1.077 to the bird respiration CO 2 balance VR. Hence, the CO 2 balance or concentration difference method offers a viable alternative or supplemental check for quantifying building VR under certain conditions where direct, continuous VR measurement is not feasible.

SUMMARY Practical means to decrease aerial emissions will enhance the ability of the US egg industry to improve environmental stewardship while continuing to provide consumers safe and affordable eggs. Ammonia emissions from manure-belt laying hen houses have been shown to be less than 10% of the emissions from high-rise counterparts where manure is stored in-house for a year. However, on-farm manure storage for manure-belt houses also emits NH3, which is a part of the total farm emissions. Nevertheless, treating manure in storage sheds to decrease NH3 emissions may be more readily implemented than treatment inside the layer houses because of potential bird health concerns and possible detrimental effects of the treatment on the housing equipment. The laboratory-scale experiments reported here examined the efficacy of 4 commercially available treatment agents, topically applied to laying hen manure at 3 different dosages, in decreasing NH3 emissions from the manure storage. The treatment agents included zeolite, 2 forms of Al + Clear (aluminum sulfate, 48.5% liquid and granular), Ferix-3 (ferric sulfate), and Poultry Litter Treatment (PLT, sodium bisulfate). All the tested agents showed appreciable NH3 emission reduction of 33 to 94%. In all cases, the greatest application dosage provided little additional NH3 reduction as compared with the medium dosage (P > 0.70). Comparison among the dry granular Al + Clear, Ferix-3, and PLT in reduction of NH3 emission over a 7-d manure storage period showed no significant difference when the agents were applied at 0.5 kg/m 2 of manure surface area (P = 0.40) but greater reduction for Al + Clear (92 ± 3%) and Ferix-3 (90 ± 1%) as compared with PLT (81 ± 2%) when applied at 1.0 kg/m 2 (P < 0.01). Further field verification tests of the laboratory-scale findings are warranted.

Volatile sulfur compounds (VSCs) are a major class of chemicals associated with odor from animal feeding operations (AFOs). Identifying and quantifying VSCs in air is challenging due to their volatility, reactivity, and low concentrations. In the present study, a canister-based method collected whole air in fused silica-lined (FSL) mini-canister (1.4 L) following passage through a calcium chloride drying tube. Sampled air from the canisters was removed (10–600 mL), dried, pre-concentrated, and cryofocused into a GC system with parallel detectors (mass spectrometer (MS) and pulsed flame photometric detector (PFPD)). The column effluent was split 20:1 between the MS and PFPD. The PFPD equimolar sulfur response enhanced quantitation and the location of sulfur peaks for mass spectral identity and quantitation. Limit of quantitation for the PFPD and MSD was set at the least sensitive VSC (hydrogen sulfide) and determined to be 177 and 28 pg S, respectively, or 0.300 and 0.048 μg m−3 air, respectively. Storage stability of hydrogen sulfide and methanethiol was problematic in warm humid air (25 °C, 96% relative humidity (RH)) without being dried first, however, stability in canisters dried was still only 65% after 24 h of storage. Storage stability of hydrogen sulfide sampled in the field at a swine facility was over 2 days. The greater stability of field samples compared to laboratory samples was due to the lower temperature and RH of field samples compared to laboratory generated samples. Hydrogen sulfide was the dominant odorous VSCs detected at all swine facilities with methanethiol and dimethyl sulfide detected notably above their odor threshold values. The main odorous VSC detected in aged poultry litter was dimethyl trisulfide. Other VSCs above odor threshold values for poultry facilities were methanethiol and dimethyl sulfide.

Most air quality studies in agricultural environments use thermal desorption analysis for quantifying semivolatile organic compounds (SVOCs) associated with odor. The objective of this study was to develop a robust sampling technique for measuring SVOCs in humid environments. Test atmospheres were generated at ambient temperatures (23 +/- 1.5 degrees C) and 25, 50, and 80% relative humidity (RH). Sorbent material used included Tenax, graphitized carbon, and carbon molecular sieve (CMS). Sorbent tubes were challenged with 2, 4, 8, 12, and 24 L of air at various RHs. Sorbent tubes with CMS material performed poorly at both 50 and 80% RH dueto excessive sorption of water. Heating of CMS tubes during sampling or dry-purging of CMS tubes post sampling effectively reduced water sorption with heating of tubes being preferred due to the higher recovery and reproducibility. Tenaxtubes had breakthrough of the more volatile compounds and tended to form artifacts with increasing volumes of air sampled. Graphitized carbon sorbent tubes containing Carbopack X and Carbopack C performed best with quantitative recovery of all compounds at all RHs and sampling volumes tested. The graphitized carbon tubes were taken to the field for further testing. Field samples taken from inside swine feeding operations showed that butanoic acid, 4-methylphenol, 4-ethylphenol, indole, and 3-methylindole were the compounds detected most often above their odor threshold values. Field samples taken from a poultry facility demonstrated that butanoic acid, 3-methylbutanoic acid, and 4-methylphenol were the compounds above their odor threshold values detected most often, relative humidity, CAFO, VOC, SVOC, thermal desorption, swine, poultry, air quality, odor.

Reduced biogas production due to toxicants or temperature change can be catastrophic to anaerobic digester operation. Unfortunately, farm manures and wastewaters can contain toxic sanitizers and feed additives, and undergo biomass temperature changes and long-term storage. To understand these impacts, we used mesophilic and thermophilic biomass anaerobic toxicity assays and methanogen activity assays to determine the inhibitory concentration causing a 50% decrease in methane production rate (i.e., IC50) of a group of commercial products. The products were Rumensin and 11 cleaners/sanitizers and disinfectants (teat dips) from dairy farms (Genron IV, Della Super, Tri Pfan, Zinicin, 1313-SD, Sheen Ezey, Mandate, Masticide, Artec, Della-Soft, and copper sulfate). The effects of thermophilic biomass storage duration (3 to 28 days) and temperature (4°C to 70°C) were also determined. All products were toxic, with IC50 values in the narrow range of 0.1 to 6 v/v %. The quaternary ammonium salt (QUAT)-based sanitizer (Genron IV) and Rumensin were the most toxic, whereas other products were less toxic and had similar toxicity to ammonia. There was no significant difference between mesophilic and thermophilic biomass IC50 values. At storage temperature >20°C, thermophilic digester biomass activity decreased after storage for 3 to 28 days, with approximately 75% activity loss after 28 days. In contrast, less activity loss was observed at lower temperatures (20°C and 4°C). The activity decrease after storage was from 0.9% to 1.6% per °C for the durations and temperatures tested, with greater decreases at longer durations. This information can be used to help determine a course of action to minimize adverse impacts on anaerobic digesters.

Gas analyzers are commonly protected from impurities in air sampling via use of in-line dust filters to ensure operational performance and longevity of the instruments. This is especially true with extended periods of air quality monitoring in dusty environments. Prices for commercially available filters and monitoring needs vary considerably. A question that has often come up but has not received much investigation is how the filter media types (e.g., paper vs. Teflon) and operational conditions (clean vs. dirty) impact the integrity of gaseous concentration measurement. The study reported here was conducted toward addressing this issue. Specifically, the study assessed the magnitude of ammonia (NH3) adsorption for several types of in-line filters and conditions often used or encountered in animal feeding operation air emission studies, namely, Teflon (most expensive), paper (least expensive), and stand-alone automobile fuel filters, being either clean (new) or dust-laden. Three nominal NH3 levels (20, 45, or 90 ppm, generated with poultry manure) coupled with two nominal airflow rates (4 vs. 8 L/min or 8 vs. 16 L/min) through the filters were used in the evaluation. The types of dust used in the study included corn starch and broiler-house dust. Simultaneous measurements of NH3 concentrations before and after the tested filter were made with two photoacoustic gas spectrometers. The results revealed that NH3 adsorption was highest for the fuel filter initially but negligible for the Teflon filters. However, after 30 min exposure, relative NH3 adsorption by the filters mostly fell below 1%. The higher flow rate led to significantly lower relative NH3 adsorption for both the fuel and paper filters (P < 0.001) but made no difference for the Teflon filters (P = 0.31 to 0.49). During fresh-air purging of the fuel filters laden with broiler-house dust, NH3 was initially released but diminished after 15 min. The results suggest that when used properly (e.g., proper flow rate), the in-line dust filters tested in this study (fuel, paper, and Teflon) offer viable, performance-based options for air emissions (especially NH3) measurement applications.

Alum [Al(SO4) ·14HO] addition to poultry litter has been shown to reduce ammonia (NH) concentrations in poultry houses; however, its effects on greenhouse gas (GHG; NO, CH, and CO) emissions is unknown. The objectives of this study were to determine the effects of alum additions on (i) in-house NH and GHG concentrations, (ii) NH and GHG emissions, and (iii) litter chemical properties. Two identical broiler houses located in northwest Arkansas were used for this study: one house was a control and the other was treated with alum between each flock of birds. Ventilation rates were coupled with in-house NH and GHG measurements to determine emission rates. Overall, alum additions significantly reduced the daily average in-house NH concentration by 42% (8.9 vs. 15.4 μL L), and the overall NH emission rate was reduced by 47% (7.2 vs. 13.4 kg d house). The average cumulative NH emission for the three flocks was 330 kg house flock for the alum-treated house and 617 kg house flock for the control. Concentrations and emissions of nitrous oxide (NO) and methane (CH) from the alum-treated house were not significantly different than the untreated house. However, carbon dioxide (CO) emissions were significantly higher from the untreated house than the alum-treated house. Alum also significantly increased litter N content and reduced the C/N ratio. These results indicate that the addition of alum to poultry litter is not only an effective management practice for reducing in-house NH concentrations and emissions but also significantly reduces CO emissions from poultry facilities.

Complex variation in gas emissions from animal facilities has been shown in recent research reports. Uncertainties in these emission estimates are driving research activities concerning different animal species across the globe. Greenhouse gas (NO and CO) and NH concentrations were measured in a modern, tunnel-ventilated, commercial broiler house in Mississippi during five flocks (spanning approximately 1 yr). These were flocks 9 through 13 on reused pine shavings litter, representing litter reuse beyond 2 yr. Gas concentrations obtained from a photoacoustic multigas analyzer were coupled with ventilation measurements of air flow through the house to develop NH and NO emission rates. Ammonia emission during a flock (43 d) averaged approximately 14.8 ± 9.8 kg d in the commercial house (equivalent to 23.5 g bird marketed or 0.54 g bird d). Nitrous oxide emission averaged 2.3 ± 1.7 kg d in the house (equivalent to 3.64 g bird marketed or 0.085 g bird d). Emission rates increased with time from Day 1 to Day 43 and reached average values on Day 23 and 24 for NH and NO. Even with extended litter reuse, estimates of NH emissions from the broiler house agree well with recently published research that reused litter in eight or fewer flocks. This is important information for farmers who may not be able to afford to replace the litter with fresh bedding material annually.

Naturally occurring estrogens in animal wastes may cause negative environmental impacts, yet their abundance in animal waste treatment and storage structures is poorly documented. To better quantify estrogen concentrations in animal wastes, multiple waste samples were collected from treatment and storage structures at dairy and swine facilities and analyzed for concentrations of 17beta-estradiol (E2), estrone (E1), and 17alpha-estradiol by gas chromatography-mass spectroscopy and by enzyme linked immunosorbent assay (E2 only). Mass ratios of each estrogen to the macronutrients nitrogen, phosphorus, and potassium were also determined. Because manure application rates are typically macronutrient-based, estrogen to macronutrient ratios are proportional to areal mass application rates of estrogen to fields. Swine farrowing waste (from farrowing sows and piglets) had the highest ratios of E2 to macronutrients. Mean ratios in swine farrowing waste were roughly twice those in swine finishing waste (from growing male and nonpregnant female animals) and more than four times higher than those in dairy waste (from lactating cows in various stages of their reproductive cycles); these differences were statistically significant (alpha = 0.05). Estrone followed a similar trend. In contrast, ratios of 17alpha-estradiol to macronutrients were highest in dairy operations. These results can be used to better predict estrogen loading rates on fields receiving swine and dairy wastes.

Both subsurface flow and free-water surface constructed wetland systems have been used for partial treatment of manure-laden wastewater from animal production systems. Subsurface flow systems are considerably more expensive but do not breed mosquitoes. The less expensive free-water surface systems have significant mosquito production potential, which is a serious drawback, especially because of increasing concerns about emerging mosquito-borne disease organisms. Periodically draining constructed wetlands has been suggested as a method of mosquito control. To test this approach, eight free-water surface constructed wetland mesocosms (0.7 m2 each) were operated on a one-week drain/fill cycle. Simultaneously, four subsurface flow mesocosms were operated continuously, to compare nitrogen, phosphorus, and organic matter removal between the two systems. Mosquito populations (larvae, pupae, and emerged adults) were monitored in the free-water surface cells, with results demonstrating that periodic draining prevents mosquito emergence in all but high-rainfall conditions. During high-rainfall periods, supplemental drainage or chemical controls might be required to prevent mosquito emergence. Pollutant removal rates, expressed as mass removal rate per unit area, were similar for the free-water surface cells and the subsurface flow cells.

Laboratory experiments were conducted using magnesium chloride (MgCl2·6H2O, 64% solution) to force the precipitation of phosphorus and reduce the concentration of soluble phosphorus (PO43-) in two swine wastes. One of the swine wastes tested contained a high concentration of PO43- (initially ≈1,000 mg/L), and the other swine waste tested contained a low concentration of PO43- (initially ≈230 mg/L). The precipitation reactions were performed to determine the required reaction time, pH, magnesium addition rate and seed material for future precipitate recovery work. For the high and low concentration waste, a 10-minute reaction time at a pH of 8.6 was sufficient to remove 98 and 96% of the PO43- from solution. A molar ratio of Mg2+:PO43- of 1.6:1 was determined to be effective for PO43- removal from both the low and high strength wastes. At a molar ratio of 1.6:1, the PO3- in the high concentration waste was reduced from 590 to 12 mg/L. In the low concentration waste, the PO43- concentration was reduced from 157 to 15 mg/L. Seeding the reaction did not significantly enhance the recovery process.

Fourteen years of accumulated electrical energy use data related to milking operations at the 160-cow University of Tennessee Dairy Experiment Station in Lewisburg, Tennessee were analyzed to quantify relative effects of production -related factors on energy use within the milking center. Energy use was measured for vacuum pumps, refrigeration compressors, water heaters, and an air compressor. These energy use figures were then related to the recorded production parameters: number of cows milked, the amount of milk sold, percent butterfat of the milk, the total pounds of fat corrected milk, and high and low dry bulb temperature by means of multiple regression. Analyses were made on a monthly and yearly basis. Total dairy energy use could not be computed due to numerous non-dairy load components in the Experiment Station that could not be subtracted. It followed logically that number of cows milked, milk production, and weather conditions represented by dry bulb temperature were found to be the major factors affecting energy use. However, no parameter, or combination of parameters, explained in a statistical sense more than 74% of the variability in the recorded energy use by the equipment studied. Quantity, and not quality, of milk produced was the most significant factor affecting energy use, with the number of cows milked and outside temperatures playing lesser roles. Regression results showing that cow numbers was not a factor related to energy use for milk cooling or vacuum pump operation, lead to some questions about the validity of kWh/cow/yr as an energy use indicator. For the 14 years of data available, average EUIs for the vacuum pumps for milking (1.10 kWh/cwt) and the refrigeration compressors for milk cooling (1.02 kWh/cwt) were near the upper range of published reference values (0.4 to 1.19 kWh/cwt and 0.8 to 1.10 kWh/cwt, respectively), while average EUI for water heaters (0.65 kWh/cwt) were well below the published range (0.95 to 1.40 kWh/cwt). The former two results confirm the validity of the published EUIs that were based only on a few year’s data. Interestingly, the results also indicate a general consistency in the year to year operation of similarly sized dairy operations. The lower EUI for the water heater was due to the use of a preheater and also the every-other-day milk collection at the dairy.

Laboratory and field experiments were conducted using magnesium chloride (MgCl2) to force the precipitation of struvite (MgNH4PO4 x 6H2O) and reduce the concentration of soluble phosphorus (SP) in swine waste. In laboratory experiments, reductions of SP of 76% (572 to 135 mg P l(-1)) were observed in raw swine manure after addition of magnesium chloride (MgCl2) at a rate calculated to provide a 1.6:1 molar ratio of magnesium (Mg) to total phosphorus. Adjusting the pH of the treated manure to pH 9.0 with sodium hydroxide (NaOH) increased SP reduction to 91% (572 to 50 mg P l(-1)). X-ray diffraction of the precipitate recovered from swine waste slurry treated only with MgCl2 confirmed the presence ofstruvite. The molar N:P:Mg ratio of the recovered precipitate was 1:1.95:0.24, suggesting that compounds in addition to struvite were formed. In a field experiment conducted in a swine manure holding pond, a 90% reduction in SP concentration was observed in approximately 140,000 l of swine manure slurry treated before land application with 2,000 l MgCl2 (64% solution) at ambient slurry temperatures ranging from 5 to 10 degrees C.