1. Improvements in lung function following vitamin C supplementation to pregnant smokers are associated with buccal DNA methylation at 5 years of age
- Author
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Lyndsey E. Shorey-Kendrick, Cindy T. McEvoy, Kristin Milner, Julia Harris, Julie Brownsberger, Robert S. Tepper, Byung Park, Lina Gao, Annette Vu, Cynthia D. Morris, and Eliot R. Spindel
- Subjects
MSDP: maternal smoking during pregnancy ,Vitamin C ,RCT: randomized clinical trial ,DNA methylation ,MethylationEPIC ,Nicotine ,Medicine ,Genetics ,QH426-470 - Abstract
Abstract Background We previously reported in the “Vitamin C to Decrease the Effects of Smoking in Pregnancy on Infant Lung Function” randomized clinical trial (RCT) that vitamin C (500 mg/day) supplementation to pregnant smokers is associated with improved respiratory outcomes that persist through 5 years of age. The objective of this study was to assess whether buccal cell DNA methylation (DNAm), as a surrogate for airway epithelium, is associated with vitamin C supplementation, improved lung function, and decreased occurrence of wheeze. Methods We conducted epigenome-wide association studies (EWAS) using Infinium MethylationEPIC arrays and buccal DNAm from 158 subjects (80 placebo; 78 vitamin C) with pulmonary function testing (PFT) performed at the 5-year visit. EWAS were performed on (1) vitamin C treatment, (2) forced expiratory flow between 25 and 75% of expired volume (FEF25–75), and (3) offspring wheeze. Models were adjusted for sex, race, study site, gestational age at randomization (≤ OR > 18 weeks), proportion of epithelial cells, and latent covariates in addition to child length at PFT in EWAS for FEF25–75. We considered FDR p
- Published
- 2024
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