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323 results on '"Robbins, Phillips W."'

36. Molecular analysis of the Candida albicans homolog of Saccharomyces cerevisiae MNN9, required for glycosylation of cell wall mannoproteins

Author

Southard, Susan B., Specht, Charles A., Mishra, Chitra, Chen-Weiner, Joan, and Robbins, Phillips W.

Subjects
Bacteriology -- Research, Cell research -- Analysis, Glycosylation -- Research, Candida albicans -- Research, Saccharomyces -- Research, Fungi -- Physiological aspects, Biological sciences
Abstract

A study of the genes that encode glucan and chitin in fungal pathogens has been carried out. The role of MNN9 in glycosylation in Candida albicans and Saccharomyces cerevisiae is discussed.

Published
1999

41. Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae

Author

Lussier, Marc, White, Ann-Marie, Sheraton, Jane, Di Paolo, Tiziano, Treadwell, Julie, Southard, Susan B., Horenstein, Craig I., Chen-Weiner, Joan, Ram, Arthur F.J., Kapteyn, Johan C., Roemer, Terry W., Vo. Dahn H., Bondoc, Dana C., Hall, John, Zhong, Wu Wei, Sdicu, Anne-Marie, Davies, Julian, Klis, Frans M., Robbins, Phillips W., and Bussey, Howard

Subjects
Saccharomyces -- Genetic aspects, Gene mutations -- Research, Biosynthesis -- Research, Plasma membranes -- Research, Biological sciences
Abstract

The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes involved in cell surface assembly by screening transposon-mutagenized cells for altered sensitivity to calcofluor white, followed by supplementary screens to further characterize mutant phenotypes. The mutated genes were directly retrieved from genomic DNA and then matched uniquely to a gene in the yeast genome database. Eighty-two genes with apparent perturbation of the cell surface were identified, with mutations in 65 of them displaying at least one further cell surface phenotype in addition to their modified sensitivity to calcofluor. Fifty of these genes were previously known, 17 encoded proteins whose function could be anticipated through sequence homology or previously recognized phenotypes and 15 genes had no previously known phenotype.

Published
1997

44. Yeast glycoprotein biosynthesis: MNT1 encodes an alpha-1,2-mannosyltransferase involved in O-glycosylation

Author

Hausler, Alex, Ballou, Lun, Ballou, Clinton E., and Robbins, Phillips W.

Subjects
Glycoproteins -- Physiological aspects, Saccharomyces -- Genetic aspects, Biosynthesis -- Research, Glycosylation -- Research, Science and technology
Abstract

The Saccharomyces cerevisae gene MNT1 codes for a membrane-bound Golgi mannosyltransferase. Gene disruption experiments showed that MNT1 mutants remain viable, but are defective in the elongation of protein O-linked mannobiose. Furthermore, the MNT1 mutants showed no disruptions in the carbohydrate structure of the membrane side chains. Sequence comparisons showed that MNT1 is identical to KRE2, which in the mutated form, confer resistance to the killer toxin K1 of S. cerevisiae.

Published
1992

50. Chitin synthase III: Synthetic lethal mutants and 'stress related' chitin synthesis that bypasses the CSD3/CHS6 localization pathway

Author

OSMOND, BARBARA C., SPECHT, CHARLES A., and ROBBINS, PHILLIPS W.

Subjects
Chitin -- Research, Saccharomyces -- Genetic aspects, Gene mutations -- Research, Science and technology
Abstract

We screened Saccharomyces strains for mutants that are synthetically lethal with deletion of the major chitin synthase gene CHS3. In addition to finding, not surprisingly, that mutations in major cell wall-related genes such as FKS1 (glucan synthase) and mutations in any of the Golgi glycosylation complex genes (MNN9 family) are lethal in combination with chs3[Delta], we found that a mutation in Srv2p, a bifunctional regulatory gene, is notably lethal in the chs3 deletion. In extending studies of fks1-chitin synthase 3 interactions, we made the surprising discovery that deletion of CSD3/CHS6, a gene normally required for Chs3p delivery and activity in vivo, was not lethal with fks1 and, in fact, that lack of Csd3p/Chs6p did not decrease the high level of stress-related chitin made in the fks1 mutant. This finding suggests that 'stress response' chitin synthesis proceeds through an alternate Chs3p targeting pathway.

Published
1999

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