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2. Polymerase chain reaction--based species verification and microsatellite analysis for canine cell line validation.

Author

O'Donoghue, Liza E., Rivest, Jason P., and Duval, Dawn L.

Subjects
CELL lines, POLYMERASE chain reaction, CELL culture, VETERINARY medicine, MEDICINE
Abstract

The article examines the prevalence of inter- and intraspecies cell line contamination in a veterinary research setting. The results showed that 3 canine cell lines were misindentified or cross-contaminated by other canine cell lines. It emphasizes the importance of cell line validation as a component of good cell culture practice.

Published
2011
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