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Ambrosia beetles (Coleoptera: Curculionidae: Scolytinae and Platypodinae) bore into tree xylem to complete their life cycle, feeding on symbiotic fungi. Ambrosia beetles are a threat to avocado where they have been found to vector a symbiotic fungus, Raffaelea lauricola, the causal agent of the laurel wilt disease. We assessed the repellency of methyl salicylate and verbenone to two putative laurel wilt vectors in avocado, Xyleborus volvulus (Fabricius) and Xyleborus bispinatus (Eichhoff), under laboratory conditions. Then, we tested the same two chemicals released from SPLAT flowable matrix with and without low-dose ethanol dispensers for manipulation of ambrosia beetle populations occurring in commercial avocado. The potential active space of repellents was assessed by quantifying beetle catch on traps placed 'close' (~5-10 cm) and 'far' (~1-1.5 m) away from repellent dispensers. Ambrosia beetles collected on traps associated with all in-field treatments were identified to species to assess beetle diversity and community variation. Xyleborus volvulus was not repelled by methyl salicylate (MeSA) or verbenone in laboratory assays, while X. bispinatus was repelled by MeSA but not verbenone. Ambrosia beetle trap catches were reduced in the field more when plots were treated with verbenone dispensers (SPLAT) co-deployed with low-dose ethanol dispensers than when treated with verbenone alone. Beetle diversity was highest on traps deployed with low-dose ethanol lures. The repellent treatments and ethanol lures significantly altered the species composition of beetles captured in experiment plots. Our results indicate that verbenone co-deployed with ethanol lures holds potential for manipulating ambrosia beetle vectors via push-pull management in avocado. This tactic could discourage immigration and/or population establishment of ambrosia beetles in commercial avocado and function as an additional tool for management programs of laurel wilt.

Ambrosia beetles (Coleoptera: Curculionidae: Scolytinae) are among the most devastating pests of orchards, nurseries, and forests. Improving trap design and ethanol lures for capturing ambrosia beetles is necessary to develop effective monitoring and management strategies. In this 2-year study, we assessed 4 trap designs and 3 commercially formulated ethanol lures to refine trapping methods tailored for orchard environments in the eastern United States. Our investigation included orchards in 2 regions, Georgia (pecan orchards) and New York (apple orchards), targeting major ambrosia beetle (Coleoptera: Curculionidae) pest species such as Xylosandrus crassiusculus (Motschulsky), X. compactus (Eichhoff), X. germanus (Blandford), and Anisandrus maiche (Stark). Among the trap designs evaluated, clear sticky cards were most effective for capturing ambrosia beetles across orchard locations. Notably, in Georgia, sticky cards paired with specific low-release ethanol lures demonstrated enhanced capture of X. crassiusculus and X. compactus , 2 key ambrosia beetle pests found infesting young pecan trees. Similarly, in New York, sticky cards baited with low-release ethanol lures captured the highest rates of X. germanus and A. maiche , thus indicating its suitability for diverse ambrosia beetle populations. Overall, our study provides practical implications for tailoring trapping protocols to optimize ambrosia beetle management strategies in orchard settings. [ABSTRACT FROM AUTHOR]

The Asian citrus psyllid, Diaphorina citri Kuwayama, transmits the bacterial pathogen associated with huanglongbing, a fatal citrus disease. Alternatives to insecticides for D. citri management are being explored, given the development of insecticide resistance and the lack of efficient biological controls. Essential oils as pest repellents have shown some efficacy in laboratory and field evaluations; however, lack of an effective dispenser for field deployment has prevented their application. In this investigation, we evaluated glycerin‐based dispensers releasing fir (Abies spp.) oil as repellents for D. citri. In olfactometer tests, glycerin dispensers loaded with fir oil at 20% w/w repelled psyllids from citrus odours but did not cause repellency at lower loading dosages. Field evaluations were conducted with dispensers at the 20% loading dosage based on laboratory results. In open‐air cage bioassays, fewer psyllids alighted on trees treated with 2 dispensers per tree than on control trees. Similarly, fewer psyllids were found on mature lime trees in homeowner landscapes treated with 4 dispensers per tree compared to paired control trees. In small plot experiments in commercial grapefruit, psyllid infestation of young leaf flush shoots was reduced in plots treated with at least 250 dispensers/ha compared to untreated control plots. Our results indicate that these glycerin‐based fir oil dispensers can reduce infestation of citrus by D. citri. However, the efficacy observed within replicated small plots of citrus grown in monoculture was judged insufficient to manage this vector as a stand‐alone treatment. Larger scale evaluations at higher loading dosages of fir oil and/or in combination with other management tools are warranted based on the outcomes of these initial experiments. [ABSTRACT FROM AUTHOR]

The Asian citrus psyllid, Diaphorina citri, is the vector of the pathogenic bacteria that causes Huanglongbing, the most devasting disease affecting citrus worldwide. As cultivation of citrus tends to expand northward, the tolerance of psyllids to freezing temperatures needs to be investigated. While mortality of D. citri to cold temperature has been previously studied, much less is known regarding the acclimation potential of psyllids to cold temperatures. We first evaluated cold resistance of D. citri depending on color morph and sex. Subsequently, we compared mortality of D. citri at −4 °C for 10 hours between unacclimated psyllids, psyllids that were gradually acclimated to cold over 1 or 2 weeks, and psyllids intermittently acclimated also for 1 or 2 weeks. We did not find major differences in cold resistance between color morphs and sex. We found that after 1 week of gradual acclimation, the survival of psyllids at −4 °C increased to 86%, compared to 20% in the control group. Survival did not increase after the second week of gradual acclimation. Although intermittent acclimation improved survival compared to the control group, it was less effective than gradual acclimation with a survival at 30% and 70% after 1 and 2 weeks of acclimation, respectively, although this difference with gradual acclimation was not significant at week 2. These data show that gradual cold acclimation allows D. citri to survive brief periods below freezing. It may serve as a mechanism responsible for increasing northern establishment of this pest. [ABSTRACT FROM AUTHOR]

Wind directly influences the spread of vector-borne plant pathogens by driving the passive dispersal of vectors to potentially new areas. Here, we evaluated the effect of wind speed and direction on the dispersal of the Asian citrus psyllid (ACP), Diaphorina citri (Kuwayama) (Hemiptera: Psyllidae), the vector of the bacteria causing huanglongbing (HLB), a lethal disease of citrus. The effect of different wind speeds on short or long-distance dispersal of ACP was investigated using a high-speed wind tunnel under laboratory conditions. The effect of wind direction on ACP dispersal under field conditions was evaluated using custom-made wind vane-style traps. In wind tunnel assays, ACP remained on plants until wind treatments reached ≥48 km/h when psyllids were mostly dislodged from plants and moved by the wind. For a short-distance, wind-driven movement (movement by the wind from one plant to another), the effect of wind speed was not significant at any of the wind speed treatments tested. Wind vane traps placed in a Florida citrus grove captured significantly more ACP on the windward side, suggesting that ACP were moved with the wind. The number of ACP found on the windward side of traps was significantly higher from May to August. These results indicate that ACP is likely to disperse with prevailing wind direction and that settled ACP may become dislodged and moved at random by high wind speeds occurring in areas of significant citrus production (southern California, Florida, or Texas). [ABSTRACT FROM AUTHOR]

We investigated feeding and oviposition behavior of the Asian citrus psyllid, Diaphorina citri, when exposed to the foraging trails of the convergens ladybird beetle, Hippodamia convergens. Diaphorina citri females feeding on citrus leaves directly exposed to the ladybird adults or treated with trail extract excreted significantly less honeydew droplets than controls. The trail chemicals of the ladybird beetle also decreased oviposition by D. citri females on citrus. In a no-choice experiment, D. citri females preferred to oviposit on control flush and plants than those with ladybird trail-extract treatments. In two-choice experiments, 68.0% of D. citri released into cages exhibited strong selection preference for settling and eventual oviposition on control plants than plants treated with ladybird trail extract. Diaphorina citri eggs were found on all new leaf flush of control plants, whereas only 29.5% of flush on treatment plants were selected for oviposition. The trail chemical deposited by the convergens ladybird beetle elicits repellency of D. citri feeding and oviposition. Therefore, the trail chemicals my contain components that could be useful for behavior-based management of D. citri and HLB disease by reducing psyllid feeding and oviposition. [ABSTRACT FROM AUTHOR]

We compared the flight activity of Xyleborus glabratus Eichhoff, vector and symbiont of the causal agent of laurel wilt disease (Raffaelea lauricola), with a native species Monarthrum mali (Fitch) using flight mills. Flight mills were operated either for 24h or for three 3-h time intervals. During the 3-h interval experiment, the shortest time to flight initiation for X. glabratus occurred at 1600-1900 hours. The average flight time and total flying distance during 1600-2100hours were also higher than those quantified during the other two recording times investigated. However, total flight duration and proportion of fliers was highest at 1000-1300 hours. We compared several flight parameters. About 64.0% of tested X. glabratus flew <20 m. During 24-h recording periods, M. mali flew longer distances than X. glabratus. Over 50.0%of M. mali flew over 100 mon the flight mill. Xyleborus glabratus flight activity was greatest between 1200 and 1800hours, while M. mali flew most frequently between 1500 and 2100 hours. Monarthrum mali flew more than five times more frequently than X. glabratus, and their longest single flight distance (37.5612.5 m) and total flight distance (213.7685.5 m) were greater than those of X. glabratus. These data will be useful for development of species-specific control and monitoring protocols for these ambrosia beetles based on greater understanding of their flight capacities and associated invasion distance. [ABSTRACT FROM AUTHOR]

Ambrosia beetles (Coleoptera: Curculionidae: Scolytinae) are among the most devastating pests of orchards, nurseries, and forest ecosystems. Anisandrus maiche (Kurentzov) has been regularly captured in ethanol-baited traps along woody edges of apple (Malus domestica (Suckow) Borkh.) orchards in western New York alongside a known apple pest, Xylosandrus germanus (Blandford). However, this species has yet to be a confirmed pest of apple trees. In winter 2024, we conducted a series of dissections of potted apple trees with visible ambrosia beetle attacks (i.e. entry holes, exit holes). Our examination revealed the presence of active A. maiche galleries housing mainly larvae, a few adults, and the fungal symbiont Ambrosiella cleistominuta C. Mayers & T.C. Harr This is a first report of Anisandrus maiche attacking apple trees.