45 results on '"Riu, Anne"'
Search Results
2. Investigation of the effect of hepatic metabolism on off-target cardiotoxicity in a multi-organ human-on-a-chip system
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Oleaga, Carlota, Riu, Anne, Rothemund, Sandra, Lavado, Andrea, McAleer, Christopher W., Long, Christopher J., Persaud, Keisha, Narasimhan, Narasimhan Sriram, Tran, My, Roles, Jeffry, Carmona-Moran, Carlos A., Sasserath, Trevor, Elbrecht, Daniel H., Kumanchik, Lee, Bridges, L. Richard, Martin, Candace, Schnepper, Mark T., Ekman, Gail, Jackson, Max, Wang, Ying I., Note, Reine, Langer, Jessica, Teissier, Silvia, and Hickman, James J. more...
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- 2018
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Catalog
3. New genome scale network modeling and mining workflow for detecting metabolic changes induced by exposure to chemicals
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Fresnais, Louison, primary, Perin, Olivier, additional, Riu, Anne, additional, Grall, Romain, additional, Ott, Alban, additional, Fromenty, Bernard, additional, Gallardo, Jean-Clément, additional, Stingl, Maximilian, additional, Frainay, Clément, additional, Jourdan, Fabien, additional, and Poupin, Nathalie, additional more...
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- 2023
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4. Lxr regulates lipid metabolic and visual perception pathways during zebrafish development
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Pinto, Caroline Lucia, Kalasekar, Sharanya Maanasi, McCollum, Catherine W., Riu, Anne, Jonsson, Philip, Lopez, Justin, Swindell, Eric C., Bouhlatouf, Abdel, Balaguer, Patrick, Bondesson, Maria, and Gustafsson, Jan-Åke more...
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- 2016
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5. Peroxisome Proliferator-Activated Receptor γ Is a Target for Halogenated Analogs of Bisphenol A
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Riu, Anne, Grimaldi, Marina, le Maire, Albane, Bey, Gilbert, Phillips, Kevin, Boulahtouf, Abdelhay, Perdu, Elisabeth, Zalko, Daniel, Bourguet, William, and Balaguer, Patrick
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- 2011
6. Time-of-Flight Secondary Ion Mass Spectrometry Imaging Demonstrates the Specific Localization of Deca-Bromo-Diphenyl-Ether Residues in the Ovaries and Adrenal Glands of Exposed Rats
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Seyer, Alexandre, Riu, Anne, Debrauwer, Laurent, Bourgès-Abella, Nathalie, Brunelle, Alain, Laprévote, Olivier, and Zalko, Daniel
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- 2010
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7. Exposure assessment of French women and their newborn to brominated flame retardants: Determination of tri- to deca- polybromodiphenylethers (PBDE) in maternal adipose tissue, serum, breast milk and cord serum
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Antignac, Jean-Philippe, Cariou, Ronan, Zalko, Daniel, Berrebi, Alain, Cravedi, Jean-Pierre, Maume, Daniel, Marchand, Philippe, Monteau, Fabrice, Riu, Anne, Andre, François, and Le Bizec, Bruno
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- 2009
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8. Disposition and metabolic profiling of [14C]-Decabromodiphenyl ether in pregnant Wistar rats
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Riu, Anne, Cravedi, Jean-Pierre, Debrauwer, Laurent, Garcia, Aurélie, Canlet, Cécile, Jouanin, Isabelle, and Zalko, Daniel
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- 2008
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9. Exposure assessment of French women and their newborns to tetrabromobisphenol-A: Occurrence measurements in maternal adipose tissue, serum, breast milk and cord serum
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Cariou, Ronan, Antignac, Jean-Philippe, Zalko, Daniel, Berrebi, Alain, Cravedi, Jean-Pierre, Maume, Daniel, Marchand, Philippe, Monteau, Fabrice, Riu, Anne, Andre, François, and bizec, Bruno Le
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- 2008
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10. Halogenated Bisphenol-A Analogs Act as Obesogens in Zebrafish Larvae (Danio rerio)
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Riu, Anne, McCollum, Catherine W., Pinto, Caroline L., Grimaldi, Marina, Hillenweck, Anne, Perdu, Elisabeth, Zalko, Daniel, Bernard, Laure, Laudet, Vincent, Balaguer, Patrick, Bondesson, Maria, and Gustafsson, Jan-Ake more...
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- 2014
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11. Coexposure to Phytoestrogens and Bisphenol A Mimics Estrogenic Effects in an Additive Manner
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Katchy, Anne, Pinto, Caroline, Jonsson, Philip, Nguyen-Vu, Trang, Pandelova, Marchela, Riu, Anne, Schramm, Karl-Werner, Samarov, Daniel, Gustafsson, Jan-Åke, Bondesson, Maria, and Williams, Cecilia
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- 2014
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12. Folic acid supplementation rescues valproic acid‐induced developmental neurotoxicity and behavioral alterations in zebrafish embryos
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Muhsen, Maram, primary, Youngs, Jaclyn, additional, Riu, Anne, additional, Gustafsson, Jan‐Åke, additional, Kondamadugu, Vijay Sai, additional, Garyfalidis, Elefterios, additional, and Bondesson, Maria, additional more...
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- 2021
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13. Biotransformation of the flame retardant tetrabromo-bisphenol A by human and rat sub-cellular liver fractions
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Zalko, Daniel, Prouillac, Caroline, Riu, Anne, Perdu, Elisabeth, Dolo, Laurence, Jouanin, Isabelle, Canlet, Cécile, Debrauwer, Laurent, and Cravedi, Jean-Pierre
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- 2006
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14. Parallel Biotransformation of Tetrabromobisphenol A in Xenopus laevis and Mammals
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Fini, Jean-Baptiste, Riu, Anne, Debrauwer, Laurent, Hillenweck, Anne, Le Mével, Sébastien, Chevolleau, Sylvie, Boulahtouf, Abdelaye, Palmier, Karima, Balaguer, Patrick, Cravedi, Jean-Pierre, Demeneix, Barbara A., and Zalko, Daniel more...
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- 2012
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15. Characterization of Novel Ligands of ERα, Erβ, and PPARγ: The Case of Halogenated Bisphenol A and Their Conjugated Metabolites
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Riu, Anne, le Maire, Albane, Grimaldi, Marina, Audebert, Marc, Hillenweck, Anne, Bourguet, William, Balaguer, Patrick, and Zalko, Daniel
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- 2011
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16. Probing new approaches using atmospheric pressure photo ionization for the analysis of brominated flame retardants and their related degradation products by liquid chromatography–mass spectrometry
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Debrauwer, Laurent, Riu, Anne, Jouahri, Majdouline, Rathahao, Estelle, Jouanin, Isabelle, Antignac, Jean-Philippe, Cariou, Ronan, Le Bizec, Bruno, and Zalko, Daniel
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- 2005
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17. Microphysiological heart–liver body-on-a-chip system with a skin mimic for evaluating topical drug delivery
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Pires de Mello, Camilly P., primary, Carmona-Moran, Carlos, additional, McAleer, Christopher W., additional, Perez, Julian, additional, Coln, Elizabeth A., additional, Long, Christopher J., additional, Oleaga, Carlota, additional, Riu, Anne, additional, Note, Reine, additional, Teissier, Silvia, additional, Langer, Jessica, additional, and Hickman, James J., additional more...
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- 2020
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18. Human-on-a-Chip Systems: Long-Term Electrical and Mechanical Function Monitoring of a Human-on-a-Chip System (Adv. Funct. Mater. 8/2019)
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Oleaga, Carlota, primary, Lavado, Andrea, additional, Riu, Anne, additional, Rothemund, Sandra, additional, Carmona-Moran, Carlos A., additional, Persaud, Keisha, additional, Yurko, Andrew, additional, Lear, Jennifer, additional, Narasimhan, Narasimhan Sriram, additional, Long, Christopher J., additional, Sommerhage, Frank, additional, Bridges, Lee Richard, additional, Cai, Yunqing, additional, Martin, Candace, additional, Schnepper, Mark T., additional, Goswami, Arindom, additional, Note, Reine, additional, Langer, Jessica, additional, Teissier, Silvia, additional, Cotovio, José, additional, and Hickman, James J., additional more...
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- 2019
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19. An untargeted metabolomics approach to explore the metabolic modulation of HepG2 cells exposed to low doses of bisphenol A and 17β-estradiol 2
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Cabaton, Nicolas J., Poupin, Nathalie, Canlet, Cécile, Tremblay-Franco, Marie, Audebert, Marc, Cravedi, Jean Pierre, Riu, Anne, Jourdan, Fabien, Zalko, Daniel, Métabolisme et Xénobiotiques (ToxAlim-MeX), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Analyse de Xénobiotiques, Identification, Métabolisme (E20 Metatoul-AXIOM), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-MetaToul-MetaboHUB, Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), French funding program ANR (KISMET) [CESA 008 01], Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-MetaToul-MetaboHUB, Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), and Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) more...
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Bisphenol A ,metabolic network analysis ,Estradiol ,Multivariate analysis ,[SDV]Life Sciences [q-bio] ,HepG2 cells ,Endocrine disruptors ,metabolomics - Abstract
International audience; The model xeno-estrogen bisphenol A (BPA) has been extensively studied over the past two decades, contributing to major advances in the field of endocrine disrupting chemicals research. Besides its well documented adverse effects on reproduction and development observed in rodents, latest studies strongly suggest that BPA disrupts several endogenous metabolic pathways, with suspected steatogenic and obesogenic effects. BPA's adverse effects on reproduction are attributed to its ability to activate estrogen receptors (ERs), but its effects on metabolism and its mechanism(s) of action at low doses are so far only marginally understood. Metabolomics based approaches are increasingly used in toxicology to investigate the biological changes induced by model toxicants and chemical mixtures, to identify markers of toxicity and biological effects. In this study, we used proton nuclear magnetic resonance (1H-NMR) based untargeted metabolite profiling, followed by multivariate statistics and computational analysis of metabolic networks to examine the metabolic modulation induced in human hepatic cells (HepG2) by an exposure to low and very low doses of BPA (10-6M, 10-9M, and 10-12M), vs. the female reference hormone 17β-estradiol (E2, 10-9M, 10-12M, and 10-15M). Metabolomic analysis combined to metabolic network reconstruction highlighted different mechanisms at lower doses of exposure. At the highest dose, our results evidence that BPA shares with E2 the capability to modulate several major metabolic routes that ensure cellular functions and detoxification processes, although the effects of the model xeno-estrogen and of the natural hormone can still be distinguished. more...
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- 2018
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20. An untargeted metabolomics approach to explore the metabolic modulation of HepG2 cells exposed to low doses of bisphenol A and 17β-estradiol 2
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Poupin, Nathalie, Canlet, Cecile, Tremblay Franco, Marie, Audebert, Marc, Cravedi, Jean-Pierre, Riu, Anne, Jourdan, Fabien, Zalko, Daniel, and cabaton, Nicolas
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HepG2 cells ,metabolomics ,metabolic network analysis ,Bisphenol A ,Estradiol ,Endocrine disruptors ,Multivariate analysis - Abstract
The model xeno-estrogen bisphenol A (BPA) has been extensively studied over the past two decades, contributing to major advances in the field of endocrine disrupting chemicals research. Besides its well documented adverse effects on reproduction and development observed in rodents, latest studies strongly suggest that BPA disrupts several endogenous metabolic pathways, with suspected steatogenic and obesogenic effects. BPA's adverse effects on reproduction are attributed to its ability to activate estrogen receptors (ERs), but its effects on metabolism and its mechanism(s) of action at low doses are so far only marginally understood. Metabolomics based approaches are increasingly used in toxicology to investigate the biological changes induced by model toxicants and chemical mixtures, to identify markers of toxicity and biological effects. In this study, we used proton nuclear magnetic resonance (1H-NMR) based untargeted metabolite profiling, followed by multivariate statistics and computational analysis of metabolic networks to examine the metabolic modulation induced in human hepatic cells (HepG2) by an exposure to low and very low doses of BPA (10-6M, 10-9M, and 10-12M), vs. the female reference hormone 17β-estradiol (E2, 10-9M, 10-12M, and 10-15M). Metabolomic analysis combined to metabolic network reconstruction highlighted different mechanisms at lower doses of exposure. At the highest dose, our results evidence that BPA shares with E2 the capability to modulate several major metabolic routes that ensure cellular functions and detoxification processes, although the effects of the model xeno-estrogen and of the natural hormone can still be distinguished. more...
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- 2018
21. Long‐Term Electrical and Mechanical Function Monitoring of a Human‐on‐a‐Chip System
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Oleaga, Carlota, primary, Lavado, Andrea, additional, Riu, Anne, additional, Rothemund, Sandra, additional, Carmona‐Moran, Carlos A., additional, Persaud, Keisha, additional, Yurko, Andrew, additional, Lear, Jennifer, additional, Narasimhan, Narasimhan Sriram, additional, Long, Christopher J., additional, Sommerhage, Frank, additional, Bridges, Lee Richard, additional, Cai, Yunqing, additional, Martin, Candace, additional, Schnepper, Mark T., additional, Goswami, Arindom, additional, Note, Reine, additional, Langer, Jessica, additional, Teissier, Silvia, additional, Cotovio, José, additional, and Hickman, James J., additional more...
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- 2018
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22. An Untargeted Metabolomics Approach to Investigate the Metabolic Modulations of HepG2 Cells Exposed to Low Doses of Bisphenol A and 17β-Estradiol
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Cabaton, Nicolas J., primary, Poupin, Nathalie, additional, Canlet, Cécile, additional, Tremblay-Franco, Marie, additional, Audebert, Marc, additional, Cravedi, Jean-Pierre, additional, Riu, Anne, additional, Jourdan, Fabien, additional, and Zalko, Daniel, additional more...
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- 2018
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23. Microphysiological flux balance platform unravels the dynamics of drug induced steatosis
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Ehrlich, Avner, primary, Tsytkin-Kirschenzweig, Sabina, additional, Ioannidis, Konstantinos, additional, Ayyash, Muneef, additional, Riu, Anne, additional, Note, Reine, additional, Ouedraogo, Gladys, additional, Vanfleteren, Jan, additional, Cohen, Merav, additional, and Nahmias, Yaakov, additional more...
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- 2018
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24. Long‐Term Electrical and Mechanical Function Monitoring of a Human‐on‐a‐Chip System.
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Oleaga, Carlota, Lavado, Andrea, Riu, Anne, Rothemund, Sandra, Carmona‐Moran, Carlos A., Persaud, Keisha, Yurko, Andrew, Lear, Jennifer, Narasimhan, Narasimhan Sriram, Long, Christopher J., Sommerhage, Frank, Bridges, Lee Richard, Cai, Yunqing, Martin, Candace, Schnepper, Mark T., Goswami, Arindom, Note, Reine, Langer, Jessica, Teissier, Silvia, and Cotovio, José more...
- Abstract
The goal of human‐on‐a‐chip systems is to capture multiorgan complexity and predict the human response to compounds within physiologically relevant platforms. The generation and characterization of such systems is currently a focal point of research given the long‐standing inadequacies of conventional techniques for predicting human outcome. Functional systems can measure and quantify key cellular mechanisms that correlate with the physiological status of a tissue, and can be used to evaluate therapeutic challenges utilizing many of the same endpoints used in animal experiments or clinical trials. Culturing multiple organ compartments in a platform creates a more physiologic environment (organ–organ communication). Here is reported a human 4‐organ system composed of heart, liver, skeletal muscle, and nervous system modules that maintains cellular viability and function over 28 days in serum‐free conditions using a pumpless system. The integration of noninvasive electrical evaluation of neurons and cardiac cells and mechanical determination of cardiac and skeletal muscle contraction allows the monitoring of cellular function, especially for chronic toxicity studies in vitro. The 28‐day period is the minimum timeframe for animal studies to evaluate repeat dose toxicity. This technology can be a relevant alternative to animal testing by monitoring multiorgan function upon long‐term chemical exposure.Human multiorgan in vitro platforms are designed to reproduce human in vivo complexity in vitro, reducing the need for animal experimentation and improving human prediction. Incorporating functional readouts into these systems enables closer approximation to clinical measurements, reducing the need for traditional biochemical metrics. Establishing a long‐term multiorgan platform enables studying chronic drug administration, currently difficult for in vitro models. [ABSTRACT FROM AUTHOR] more...
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- 2019
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25. Species-specific sensitivity of the fetal testis to mild analgesics
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Pinto, Caroline, Grimaldi, Marina, Boulahtouf, Abdelhay, Riu, Anne, Pakdel, Farzad, Brion, François, Ait-Aissa, Selim, Cavailles, Vincent, Bourguet, William, Gustafsson, Jan-Ake, Bondesson, Maria, Balaguer, Patrick, Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1), Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut National de l'Environnement Industriel et des Risques (INERIS), Centre de Biochimie Structurale [Montpellier] (CBS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), CRLCC Val d'Aurelle - Paul Lamarque-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 1 (UM1), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Civs, Gestionnaire more...
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[SDV.TOX.ECO] Life Sciences [q-bio]/Toxicology/Ecotoxicology ,[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
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- 2014
26. Microphysiological flux balance platform unravels the dynamics of drug induced steatosis.
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Ioannidis, Konstantinos, Ehrlich, Avner, Tsytkin-Kirschenzweig, Sabina, Cohen, Merav, Nahmias, Yaakov, Ayyash, Muneef, Riu, Anne, Note, Reine, Ouedraogo, Gladys, and Vanfleteren, Jan
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LABS on a chip ,PHARMACODYNAMICS ,FATTY degeneration ,LIVER analysis ,DRUG development ,MICROPROBE analysis ,MICROFLUIDICS ,PREVENTION - Abstract
Drug development is currently hampered by the inability of animal experiments to accurately predict human response. While emerging organ on chip technology offers to reduce risk using microfluidic models of human tissues, the technology still mostly relies on end-point assays and biomarker measurements to assess tissue damage resulting in limited mechanistic information and difficulties to detect adverse effects occurring below the threshold of cellular damage. Here we present a sensor-integrated liver on chip array in which oxygen is monitored using two-frequency phase modulation of tissue-embedded microprobes, while glucose, lactate and temperature are measured in real time using microfluidic electrochemical sensors. Our microphysiological platform permits the calculation of dynamic changes in metabolic fluxes around central carbon metabolism, producing a unique metabolic fingerprint of the liver's response to stimuli. Using our platform, we studied the dynamics of human liver response to the epilepsy drug Valproate (Depakine™) and the antiretroviral medication Stavudine (Zerit™). Using E6/E7
LOW hepatocytes, we show TC50 of 2.5 and 0.8 mM, respectively, coupled with a significant induction of steatosis in 2D and 3D cultures. Time to onset analysis showed slow progressive damage starting only 15–20 hours post-exposure. However, flux analysis showed a rapid disruption of metabolic homeostasis occurring below the threshold of cellular damage. While Valproate exposure led to a sustained 15% increase in lipogenesis followed by mitochondrial stress, Stavudine exposure showed only a transient increase in lipogenesis suggesting disruption of β-oxidation. Our data demonstrates the importance of tracking metabolic stress as a predictor of clinical outcome. [ABSTRACT FROM AUTHOR] more...- Published
- 2018
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27. Effects of Bisphenol-A (BPA) on HEPG2 cells: an untargeted NMR-based metabonomics study
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Canlet, Cécile, Tremblay-Franco, Marie, Riu, Anne, Cabaton, Nicolas J., Lapeyre, Claire, Audebert, Marc, Zalko, Daniel, Analyse de Xénobiotiques, Identification, Métabolisme (E20 Metatoul-AXIOM), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-MetaToul-MetaboHUB, Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Métabolisme et Xénobiotiques (ToxAlim-MeX), Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-MetaToul-MetaboHUB, Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and ProdInra, Migration more...
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[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
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- 2012
28. Affinity purification using recombinant PXR as a tool to characterize environmental ligands
- Author
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Dagnino, Sonia, Bellet, Virginie, Grimaldi, Marina, Riu, Anne, Ait-Aissa, Selim, Cavaillès, Vincent, Fenet, Hélène, Balaguer, Patrick, Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1), Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Université de Montpellier (UM), Hydrosciences Montpellier (HSM), Institut national des sciences de l'Univers (INSU - CNRS)-Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Xénobiotiques, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Institut National de l'Environnement Industriel et des Risques (INERIS), French Research Ministery, Agence Francaise de Securite Sanitaire de l'Environnement et du Travail [AFSSET, RD-2005-007], CRLCC Val d'Aurelle - Paul Lamarque-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 1 (UM1), Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées more...
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Geologic Sediments ,Receptors, Steroid ,PXR ,AHR ,Estrogen Receptor alpha ,Pregnane X Receptor ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Ligands ,digestive system ,digestive system diseases ,Recombinant Proteins ,Cell Line ,Receptors, Aryl Hydrocarbon ,Receptors, Androgen ,Cell Line, Tumor ,[SDE]Environmental Sciences ,AFFINITY COLUMN ,Humans ,[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology ,Water Pollutants, Chemical ,AR ,ERalpha - Abstract
International audience; Many environmental endocrine disrupting compounds act as ligands for nuclear receptors. The human pregnane X receptor (hPXR), for instance, is activated by a variety of environmental ligands such as steroids, pharmaceutical drugs, pesticides, alkylphenols, polychlorinated biphenyls and polybromo diethylethers. Some of us have previously reported the occurrence of hPXR ligands in environmental samples but failed to identify them. The aim of this study was to test whether a PXR-affinity column, in which recombinant hPXR was immobilized on solid support, could help the purification of these chemicals. Using PXR ligands of different affinity (10 nM < EC50 < 10 uM), we demonstrated that the PXR-affinity preferentially column captured ligands with medium to high affinities (EC50 < 1 uM). Furthermore, by using the PXR-affinity column to analyze an environmental sample containing ERalpha, AhR, AR, and PXR activities, we show that (i) half of the PXR activity of the sample was due to compounds with medium to high affinity for PXR and (ii) PXR shared ligands with ERalpha, AR, and AhR. These findings demonstrate that the newly developed PXR-affinity column coupled to reporter cell lines represents a valuable tool for the characterization of the nature of PXR active compounds and should therefore guide and facilitate their further analysis. more...
- Published
- 2011
- Full Text
- View/download PDF
29. Characterization of Novel Ligands of ER alpha, Er beta, and PPAR gamma: The Case of Halogenated Bisphenol A and Their Conjugated Metabolites
- Author
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Riu, Anne, Le Maire, Albane, Grimaldi, Marina, Audebert, Marc, Hillenweck, Anne, Bourguet, William, Balaguer, Patrick, Zalko, Daniel, ToxAlim (ToxAlim), Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Centre de Biochimie Structurale [Montpellier] (CBS), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1), CRLCC Val d'Aurelle - Paul Lamarque-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 1 (UM1), Centre régional de lutte contre le cancer, Métabolisme et Xénobiotiques (ToxAlim-MeX), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université de Montpellier (UM), European Union network CASCADE (FOOD-CT-2003-506319), Agence Nationale de Securite Sanitaire (RD-2005-02), Programme National de Recherche sur les Perturbateurs Endocriniens, programmes 189, Agence Nationale de la Recherche ANR CES (BISCOT 2010 CESA 004 02 CONTREPERF 2010-CESA-008-03 KISMET 2008-CESA-008-01), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), and Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM) more...
- Subjects
obesity ,flame retardants ,chlorinated derivatives ,[SDV]Life Sciences [q-bio] ,in vitro ,halogenated bpa ,adipose tissue ,endocrine disruptors ,estrogen receptor alpha ,tandem mass spectrometry ,chromatography ,nuclear receptor ,endocrine disrupter ,affinity column ,metabolism ,tetrachlorobisphenol ,tetrabromobisphenol a - Abstract
International audience; The capability of the flame retardants tetrabromobisphenol A (TBBPA) and tetrachlorobisphenol A (TCBPA) to activate peroxysome proliferator-activated receptors (PPARs) alpha, beta, and gamma and estrogen receptors (ERs) alpha and beta has been recently investigated, but the activity of their biotransformation products and of their lower molecular weight analogues formed in the environment remains unexplored. The aim of this study was to investigate the relationship between the degree of halogenation of BPA analogues and their affinity and activity towards human PPAR gamma and ERs and to characterize active metabolites of major marketed halogenated bisphenols. The biological activity of all compounds was studied using reporter cell lines expressing these nuclear receptors (NRs). We used NR-based affinity columns to rapidly evaluate the binding affinity of halogenated bisphenols for PPAR gamma and ERs and to trap active metabolites of TBBPA and TCBPA formed in HepG2 cells. The agonistic potential of BPA analogs highly depends on their halogenation degree: the bulkier halogenated BPA analogs, the greater their capability to activate PPAR gamma. In addition, PPAR gamma-based affinity column, HGELN-PPAR gamma reporter cell line and crystallographic analysis clearly demonstrate that the sulfation pathway, usually considered as a detoxification process, leads for TBBPA and TCBPA, to the formation of sulfate conjugates which possess a residual PPAR gamma-binding activity. Our results highlight the effectiveness NR-based affinity columns to trap and characterize biologically active compounds from complex matrices. Polyhalogenated bisphenols, but also some of their metabolites, are potential disrupters of PPAR gamma activity. more...
- Published
- 2011
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30. Complementary analysis of infant formulae-chemical target analysis versus in vitro bioassays
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Pandelova, M., Henkelmann, B., Levy Lopez, W., Piccinelli, R., Leclercq, Christophe, Maggioni, S., Benfenati, E., Pinto, C., Bondesson, M., Gustafsson, J.A., Balaguer, Patrick, Riu, Anne, Zalko, Daniel, Cravedi, Jean Pierre, Schramm, K.W., ProdInra, Migration, ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Métabolisme et Xénobiotiques (ToxAlim-MeX), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3) more...
- Subjects
[SDV] Life Sciences [q-bio] ,alimentation ,biossay ,[SDV]Life Sciences [q-bio] ,in vitro ,infant ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2011
31. Environmental endocrine disruptors prurification by recombinant pregnane X and estrogen receptor-affinity columns
- Author
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Dagnino, Sonia, Riu, Anne, Cavailles, Vincent, Redinbo, Matthew, Ait-Aissa, Selim, Escande, Aurélie, Fenet, Hélène, Balaguer, Patrick, Hydrosciences Montpellier (HSM), Institut national des sciences de l'Univers (INSU - CNRS)-Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1), CRLCC Val d'Aurelle - Paul Lamarque-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 1 (UM1), Xénobiotiques, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), University of North Carolina [Chapel Hill] (UNC), University of North Carolina System (UNC), Institut National de l'Environnement Industriel et des Risques (INERIS), Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Civs, Gestionnaire, and Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM) more...
- Subjects
[SDE] Environmental Sciences ,[SDV.TOX.ECO] Life Sciences [q-bio]/Toxicology/Ecotoxicology ,[SDE]Environmental Sciences ,[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology ,digestive system ,digestive system diseases - Abstract
Nuclear Pregnane X Receptor (PXR) is involved in the regulation of drug-metabolism enzymes and therefore plays an essential role in the detoxification pathways. Recent studies have shown that PXR can be activated by exogenous environmental xenobiotics such as pesticides, polychlorinated biphenyls, brominated flame retardants, phyto and myco-estrogens and many more. We measured PXR activity in samples from wastewater treatment and surface water. However, due to the capacity of PXR to bind a multitude of different types of environmental pollutants, it is difficult to predict which type of molecule is responsible for the observed activity in environmental samples. The development of a tool to purify PXR ligands in an environmental complex matrix such as wastewater and sludge should improve their chemical detection. We already developed a recombinant ER alpha affinity column for the purification of estrogenic compounds from water and sediment extracts. In the same way we developed a procedure with immobilized PXR receptor to capture PXR environmental ligands. We showed that this method allowed us to isolate ER and PXR ligands in wastewater, sludge and sediment extracts, and thus facilitate their further analysis more...
- Published
- 2008
32. Coexposure to Phytoestrogens and Bisphenol A Mimics Estrogenic Effects in an Additive Manner
- Author
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Katchy, Anne, primary, Pinto, Caroline, additional, Jonsson, Philip, additional, Nguyen-Vu, Trang, additional, Pandelova, Marchela, additional, Riu, Anne, additional, Schramm, Karl-Werner, additional, Samarov, Daniel, additional, Gustafsson, Jan-Åke, additional, Bondesson, Maria, additional, and Williams, Cecilia, additional more...
- Published
- 2013
- Full Text
- View/download PDF
33. Identification of Estrogen Target Genes during Zebrafish Embryonic Development through Transcriptomic Analysis
- Author
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Hao, Ruixin, primary, Bondesson, Maria, additional, Singh, Amar V., additional, Riu, Anne, additional, McCollum, Catherine W., additional, Knudsen, Thomas B., additional, Gorelick, Daniel A., additional, and Gustafsson, Jan-Åke, additional more...
- Published
- 2013
- Full Text
- View/download PDF
34. Combined Genotoxic Effects of a Polycyclic Aromatic Hydrocarbon (B(a)P) and an Heterocyclic Amine (PhIP) in Relation to Colorectal Carcinogenesis
- Author
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Jamin, Emilien L., primary, Riu, Anne, additional, Douki, Thierry, additional, Debrauwer, Laurent, additional, Cravedi, Jean-Pierre, additional, Zalko, Daniel, additional, and Audebert, Marc, additional more...
- Published
- 2013
- Full Text
- View/download PDF
35. Affinity purification using recombinant PXR as a tool to characterize environmental ligands
- Author
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Dagnino, Sonia, primary, Bellet, Virginie, additional, Grimaldi, Marina, additional, Riu, Anne, additional, Aït-Aïssa, Sélim, additional, Cavaillès, Vincent, additional, Fenet, Hélène, additional, and Balaguer, Patrick, additional more...
- Published
- 2012
- Full Text
- View/download PDF
36. Parallel Biotransformation of Tetrabromobisphenol A in Xenopus laevis and Mammals: Xenopus as a Model for Endocrine Perturbation Studies
- Author
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Fini, Jean-Baptiste, primary, Riu, Anne, additional, Debrauwer, Laurent, additional, Hillenweck, Anne, additional, Le Mével, Sébastien, additional, Chevolleau, Sylvie, additional, Boulahtouf, Abdelaye, additional, Palmier, Karima, additional, Balaguer, Patrick, additional, Cravedi, Jean-Pierre, additional, Demeneix, Barbara A., additional, and Zalko, Daniel, additional more...
- Published
- 2011
- Full Text
- View/download PDF
37. Corrigendum to “Exposure assessment of French women and their newborn to brominated flame retardants: Determination of tri- to deca-polybromodiphenylethers (PBDE) in maternal adipose tissue, serum, breast milk and cord serum” [Environ. Pollut. 157 (2009) 164–173]
- Author
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Antignac, Jean-Philippe, primary, Cariou, Ronan, additional, Zalko, Daniel, additional, Berrebi, Alain, additional, Cravedi, Jean-Pierre, additional, Maume, Daniel, additional, Marchand, Philippe, additional, Monteau, Fabrice, additional, Riu, Anne, additional, Andre, François, additional, and Le Bizec, Bruno, additional more...
- Published
- 2010
- Full Text
- View/download PDF
38. Screening for polybrominated diphenyl ethers in biological samples by reversed-phase fast HPLC-ICP MS
- Author
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Bierła, Katarzyna, primary, Riu, Anne, additional, Debrauwer, Laurent, additional, Zalko, Daniel, additional, Bouyssiere, Brice, additional, and Szpunar, Joanna, additional
- Published
- 2010
- Full Text
- View/download PDF
39. Study of polybrominated diphenyl ethers using both positive and negative atmospheric pressure photoionization and tandem mass spectrometry
- Author
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Riu, Anne, primary, Zalko, Daniel, additional, and Debrauwer, Laurent, additional
- Published
- 2006
- Full Text
- View/download PDF
40. Structural characterization of fatty acids cationized with copper by electrospray ionization mass spectrometry under low-energy collision-induced dissociation
- Author
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Afonso, Carlos, primary, Riu, Anne, additional, Xu, Ying, additional, Fournier, Fran�oise, additional, and Tabet, Jean-Claude, additional
- Published
- 2005
- Full Text
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41. Affinity purification using recombinant PXR as a tool to characterize environmental ligands.
- Author
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Dagnino, Sonia, Bellet, Virginie, Grimaldi, Marina, Riu, Anne, Aït‐Aïssa, Sélim, Cavaillès, Vincent, Fenet, Hélène, and Balaguer, Patrick
- Subjects
LIGANDS (Biochemistry) ,NUCLEAR receptors (Biochemistry) ,PESTICIDES ,SPRAYING ,PEST control - Abstract
Many environmental endocrine disrupting compounds act as ligands for nuclear receptors. The human pregnane X receptor (hPXR), for instance, is activated by a variety of environmental ligands such as steroids, pharmaceutical drugs, pesticides, alkylphenols, polychlorinated biphenyls and polybromo diethylethers. Some of us have previously reported the occurrence of hPXR ligands in environmental samples but failed to identify them. The aim of this study was to test whether a PXR-affinity column, in which recombinant hPXR was immobilized on solid support, could help the purification of these chemicals. Using PXR ligands of different affinity (10 nM < EC50 < 10 μM), we demonstrated that the PXR-affinity preferentially column captured ligands with medium to high affinities (EC50 < 1 μM). Furthermore, by using the PXR-affinity column to analyze an environmental sample containing ERα, AhR, AR, and PXR activities, we show that (i) half of the PXR activity of the sample was due to compounds with medium to high affinity for PXR and (ii) PXR shared ligands with ERα, AR, and AhR. These findings demonstrate that the newly developed PXR-affinity column coupled to reporter cell lines represents a valuable tool for the characterization of the nature of PXR active compounds and should therefore guide and facilitate their further analysis. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 207-215, 2014. [ABSTRACT FROM AUTHOR] more...
- Published
- 2014
- Full Text
- View/download PDF
42. Combined Genotoxic Effects of a Polycyclic Aromatic Hydrocarbon (B(a)P) and an Heterocyclic Amine (PhIP) in Relation to Colorectal Carcinogenesis.
- Author
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Jamin, Emilien L., Riu, Anne, Douki, Thierry, Debrauwer, Laurent, Cravedi, Jean-Pierre, Zalko, Daniel, and Audebert, Marc
- Subjects
- *
GENETIC toxicology , *POLYCYCLIC aromatic hydrocarbons , *HETEROCYCLIC compounds , *AMINES , *COLON cancer , *CARCINOGENESIS , *ENVIRONMENTALLY induced diseases , *DIET in disease - Abstract
Colorectal neoplasia is the third most common cancer worldwide. Environmental factors such as diet are known to be involved in the etiology of this cancer. Several epidemiological studies have suggested that specific neo-formed mutagenic compounds related to meat consumption are an underlying factor involved in the association between diet and colorectal cancer. Heterocyclic amines (HCAs) and polycyclic aromatic hydrocarbons (PAHs) are known mutagens and possible human carcinogens formed at the same time in meat during cooking processes. We studied the genotoxicity of the model PAH benzo(a)pyrene (B(a)P) and HCA 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), alone or in mixture, using the mouse intestinal cell line ApcMin/+, mimicking the early step of colorectal carcinogenesis, and control Apc+/+ cells. The genotoxicity of B(a)P and PhIP was investigated using both cell lines, through the quantification of B(a)P and PhIP derived DNA adducts, as well as the use of a genotoxic assay based on histone H2AX phosphorylation quantification. Our results demonstrate that heterozygous Apc mutated cells are more effective to metabolize B(a)P. We also established in different experiments that PhIP and B(a)P were more genotoxic on ApcMin/+ cells compared to Apc+/+. Moreover when tested in mixture, we observed a combined genotoxicity of B(a)P and PhIP on the two cell lines, with an increase of PhIP derived DNA adducts in the presence of B(a)P. Because of their genotoxic effects observed on heterozygous Apc mutated cells and their possible combined genotoxic effects, both B(a)P and PhIP, taken together, could be implicated in the observed association between meat consumption and colorectal cancer. [ABSTRACT FROM AUTHOR] more...
- Published
- 2013
- Full Text
- View/download PDF
43. Peroxisome Proliferator-Activated Receptor ã Is a Target for Halogenated Analogs of Bisphenol A.
- Author
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Riu, Anne, Grimaldi, Marina, le Maire, Albane, Bey, Gilbert, Phillips, Kevinps, Boulahtouf, Abdelhay, Perdu, Elisabeth, Zalko, Daniel, Bourguet, William, and Balaguer, Patrick
- Abstract
Background: The occurrence of halogenated analogs of the xenoestrogen bisphenol A (BPA) has been recently demonstrated both in environmental and human samples. These analogs include brominated [e.g., tetrabromobisphenol A (TBBPA)] and chlorinated [e.g., tetrachlorobisphenol A (TCBPA)] bisphenols, which are both flame retardants. Because of their structural homology with BPA, such chemicals are candidate endocrine disruptors. However, their possible target(s) within the nuclear hormone receptor superfamily has remained unknown. Objectives: We investigated whether BPA and its halogenated analogs could be ligands of estrogen receptors (ERs) and peroxisome proliferator-activated receptors (PPARs) and act as endocrine-disrupting chemicals. Methods: We studied the activity of compounds using reporter cell lines expressing ERs and PPARs. We measured the binding affinities to PPARγ by competitive binding assays with [3H] rosiglitazone and investigated the impact of TBBPA and TCBPA on adipocyte differentiation using NIH3T3-L1 cells. Finally, we determined the binding mode of halogenated BPAs to PPARγ by X-ray crystallography. Results: We observed that TBBPA and TCBPA are human, zebrafish, and Xenopus PPARγ ligands and determined the mechanism by which these chemicals bind to and activate PPARγ. We also found evidence that activation of ERα, ERβ, and PPARγ depends on the degree of halogenation in BPA analogs. We observed that the bulkier brominated BPA analogs, the greater their capability to activate PPARγ and the weaker their estrogenic potential. Conclusions: Our results strongly suggest that polyhalogenated bisphenols could function as obesogens by acting as agonists to disrupt physiological functions regulated by human or animal PPARγ. [ABSTRACT FROM AUTHOR] more...
- Published
- 2011
- Full Text
- View/download PDF
44. Warm Reception? Halogenated BPA Flame Retardants and PPARγ Activation
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Kevin J. Phillips, Abdelhay Boulahtouf, William Bourguet, Elisabeth Perdu, Anne Riu, Gilbert Bey, Marina Grimaldi, Daniel Zalko, Patrick Balaguer, Albane le Maire, ToxAlim (ToxAlim), Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut de Recherche en Cancérologie de Montpellier (IRCM - U1194 Inserm - UM), CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1), CRLCC Val d'Aurelle - Paul Lamarque-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 1 (UM1), NovAliX, Métabolisme et Xénobiotiques (ToxAlim-MeX), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Centre de Biochimie Structurale [Montpellier] (CBS), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM), European Union FOOD-CT-2003-506319, Agence Nationale de Securite Sanitaire RD-2005-02, Programme National de Recherche sur les Perturbateurs Endocriniens, ECOPI 189, Agence Nationale de la Recherche, Contaminants, Ecosystemes, Sante BISCOT 2010 CESA 004 02, CONTREPERF 2010 CESA 008 03, KISMET 2008 CESA 008 01, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Riu, Anne, Grimaldi, Marina, le Maire, Albane, Bey, Gilbert, Boulahtouf, Abdelhay, Perdu, Elisabeth, Zalko, Daniel, Bourguet, William, and Balaguer, Patrick more...
- Subjects
obesity ,PPARγ ,Health, Toxicology and Mutagenesis ,Xenopus ,[SDV]Life Sciences [q-bio] ,Peroxisome proliferator-activated receptor ,BPA, endocrine disruptor, obesity, PPARγ, TBBPA, TCBPA ,010501 environmental sciences ,Endocrine Disruptors ,Crystallography, X-Ray ,Ligands ,01 natural sciences ,chemistry.chemical_compound ,TCBPA ,PPAR delta ,Receptor ,News | Science Selections ,Zebrafish ,Flame Retardants ,chemistry.chemical_classification ,endocrine disruptor ,0303 health sciences ,BPA ,TBBPA ,Xenoestrogen ,Reproductive Health ,Endocrine disruptor ,Biochemistry ,Tetrabromobisphenol A ,Peroxisome proliferator-activated receptor delta ,Bisphenol A (BPA) ,hormones, hormone substitutes, and hormone antagonists ,Chlorophenols ,medicine.medical_specialty ,endocrine system ,Polybrominated Biphenyls ,Binding, Competitive ,Cell Line ,03 medical and health sciences ,Internal medicine ,medicine ,Animals ,Estrogen Receptor beta ,Humans ,PPAR alpha ,Estrogens, Non-Steroidal ,030304 developmental biology ,0105 earth and related environmental sciences ,Endocrine Health ,urogenital system ,Research ,Public Health, Environmental and Occupational Health ,Estrogen Receptor alpha ,PPAR gamma ,Endocrinology ,Metabolism ,chemistry ,Nuclear receptor ,Estrogen receptor alpha - Abstract
International audience; Background: The occurrence of halogenated analogs of the xenoestrogen bisphenol A (BPA) has been recently demonstrated both in environmental and human samples. These analogs include brominated [e.g., tetrabromobisphenol A (TBBPA)] and chlorinated [e.g., tetrachlorobisphenol A (TCBPA)] bisphenols, which are both flame retardants. Because of their structural homology with BPA, such chemicals are candidate endocrine disruptors. However, their possible target(s) within the nuclear hormone receptor superfamily has remained unknown.Objectives: We investigated whether BPA and its halogenated analogs could be ligands of estrogen receptors (ERs) and peroxisome proliferator–activated receptors (PPARs) and act as endocrine-disrupting chemicals.Methods: We studied the activity of compounds using reporter cell lines expressing ERs and PPARs. We measured the binding affinities to PPARγ by competitive binding assays with [3H]‑rosiglitazone and investigated the impact of TBBPA and TCBPA on adipocyte differentiation using NIH3T3-L1 cells. Finally, we determined the binding mode of halogenated BPAs to PPARγ by X‑ray crystallography.Results: We observed that TBBPA and TCBPA are human, zebrafish, and Xenopus PPARγ ligands and determined the mechanism by which these chemicals bind to and activate PPARγ. We also found evidence that activation of ERα, ERβ, and PPARγ depends on the degree of halogenation in BPA analogs. We observed that the bulkier brominated BPA analogs, the greater their capability to activate PPARγ and the weaker their estrogenic potential.Conclusions: Our results strongly suggest that polyhalogenated bisphenols could function as obesogens by acting as agonists to disrupt physiological functions regulated by human or animal PPARγ. more...
- Published
- 2011
- Full Text
- View/download PDF
45. Automatic segmentation of time-lapse microscopy images depicting a live Dharma embryo.
- Author
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Zacharia E, Bondesson M, Riu A, Ducharme NA, Gustafsson JÅ, and Kakadiaris IA
- Subjects
- Animals, Automation, Chromosomes metabolism, Embryo, Nonmammalian anatomy & histology, Image Processing, Computer-Assisted methods, Microscopy, Fluorescence methods, Time-Lapse Imaging methods, Zebrafish embryology
- Abstract
Biological inferences about the toxicity of chemicals reached during experiments on the zebrafish Dharma embryo can be greatly affected by the analysis of the time-lapse microscopy images depicting the embryo. Among the stages of image analysis, automatic and accurate segmentation of the Dharma embryo is the most crucial and challenging. In this paper, an accurate and automatic segmentation approach for the segmentation of the Dharma embryo data obtained by fluorescent time-lapse microscopy is proposed. Experiments performed in four stacks of 3D images over time have shown promising results. more...
- Published
- 2011
- Full Text
- View/download PDF
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