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15 results on '"Rigato, Paula Ordonhez"'

1. Implantação da rede laboratorial para realização do ensaio de liberação de interferon-gama (IGRA) para detecção de tuberculose latente no Estado de São Paulo: primeiros passos e desafios

Author

Hong, Marisa Ailin, primary, Rigato, Paula Ordonhez, additional, Lindoso, Ana Angélica B Portela, additional, Golim, Marjorie Assis, additional, Carmo, Andréia Moreira Santos, additional, Bombonatte, Andrea Gobetti Coelho, additional, Sartori, Beatriz Gomes Carreira, additional, Soares, Claudio Sousa, additional, Costa, Paulo Inácio, additional, Garcia, Lucilene Tenorio Bezerra, additional, Aoki, Francisco Hideo, additional, Sunada, Noemia Orii, additional, Bertani, Amanda Maria Jesus, additional, and Chimara, Erica, additional

Published
2023
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2. LAMP-1 Chimeric to HIV-1 p55Gag in the Immunization of Neonate Mice Induces an Early Germinal Center Formation and AID Expression

Author

Teixeira, Franciane Mouradian Emidio, primary, Oliveira, Luana de Mendonça, additional, Pietrobon, Anna Julia, additional, Salles, Érika Machado de, additional, D’Império Lima, Maria Regina, additional, Viana, Isabelle Freire Tabosa, additional, Lins, Roberto Dias, additional, Rigato, Paula Ordonhez, additional, Marques, Ernesto Torres de Azevedo, additional, da Silva Duarte, Alberto José, additional, and Sato, Maria Notomi, additional

Published
2022
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5. The Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophils

Author

Reis, Ana Paula Carvalho, primary, Celestrino, Giovanna Azevedo, additional, Igoa, Mariana Villas Bôas, additional, Jesus, Thais Martins, additional, França, Tábata Takahashi, additional, Moreira, Daniel Valério Silva, additional, Rigato, Paula Ordonhez, additional, Sato, Paula Keiko, additional, Condino-Neto, Antonio, additional, Noronha, Irene L., additional, Dias-Melicio, Luciane Alarcão, additional, Lalwani, Pritesh Jaychand, additional, Benard, Gil, additional, and Sousa, Maria Gloria Teixeira, additional

Published
2022
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6. Clinical Characteristics and Survival Analysis in Frequent Alcohol Consumers With COVID-19

Author

Alberca, Ricardo Wesley, primary, Rigato, Paula Ordonhez, additional, Ramos, Yasmim Álefe Leuzzi, additional, Teixeira, Franciane Mouradian Emidio, additional, Branco, Anna Cláudia Calvielli, additional, Fernandes, Iara Grigoletto, additional, Pietrobon, Anna Julia, additional, Duarte, Alberto Jose da Silva, additional, Aoki, Valeria, additional, Orfali, Raquel Leão, additional, and Sato, Maria Notomi, additional

Published
2021
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9. Regulation of HIV-Gag Expression and Targeting to the Endolysosomal/Secretory Pathway by the Luminal Domain of Lysosomal-Associated Membrane Protein (LAMP-1) Enhance Gag-Specific Immune Response

Author

Godinho, Rodrigo Maciel da Costa, primary, Matassoli, Flavio Lemos, additional, Lucas, Carolina Gonçalves de Oliveira, additional, Rigato, Paula Ordonhez, additional, Gonçalves, Jorge Luiz Santos, additional, Sato, Maria Notomi, additional, Maciel, Milton, additional, Peçanha, Ligia Maria Torres, additional, August, J. Thomas, additional, de Azevedo Marques, Ernesto Torres, additional, and de Arruda, Luciana Barros, additional

Published
2014
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11. Heterologous Plasmid DNA Prime-Recombinant Human Adenovirus 5 Boost Vaccination Generates a Stable Pool of Protective Long-Lived CD8 + T Effector Memory Cells Specific for a Human Parasite, Trypanosoma cruzi

Author

Rigato, Paula Ordonhez, primary, de Alencar, Bruna C., additional, de Vasconcelos, José Ronnie C., additional, Dominguez, Mariana R., additional, Araújo, Adriano F., additional, Machado, Alexandre V., additional, Gazzinelli, Ricardo T., additional, Bruna-Romero, Oscar, additional, and Rodrigues, Mauricio M., additional

Published
2011
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13. Heterologous Plasmid DNA Prime-Recombinant Human Adenovirus 5 Boost Vaccination Generates a Stable Pool of Protective Long-Lived CD8+ T Effector Memory Cells Specific for a Human Parasite, Trypanosoma cruzi

Author

Rigato, Paula Ordonhez, de Alencar, Bruna C., de Vasconcelos, José Ronnie C., Dominguez, Mariana R., Araújo, Adriano F., Machado, Alexandre V., Gazzinelli, Ricardo T., Bruna-Romero, Oscar, and Rodrigues, Mauricio M.

Abstract

Recently, we described a heterologous prime-boost strategy using plasmid DNA followed by replication-defective human recombinant adenovirus type 5 as a powerful strategy to elicit long-lived CD8+T-cell-mediated protective immunity against experimental systemic infection of mice with a human intracellular protozoan parasite, Trypanosoma cruzi. In the present study, we further characterized the protective long-lived CD8+T cells. We compared several functional and phenotypic aspects of specific CD8+T cells present 14 or 98 days after the last immunizing dose and found the following: (i) the numbers of specific cells were similar, as determined by multimer staining or by determining the number of gamma interferon (IFN-γ)-secreting cells by enzyme-linked immunospot (ELISPOT) assay; (ii) these cells were equally cytotoxic in vivo; (iii) following in vitro stimulation, a slight decline in the frequency of multifunctional cells (CD107a+IFN-γ+or CD107a+IFN-γ+tumor necrosis factor alpha positive [TNF-α+]) was paralleled by a significant increase of CD107a singly positive cells after 98 days; (iv) the expression of several surface markers was identical, except for the reexpression of CD127 after 98 days; (v) the use of genetically deficient mice revealed a role for interleukin-12 (IL-12)/IL-23, but not IFN-γ, in the maintenance of these memory cells; and (vi) subsequent immunizations with an unrelated virus or a plasmid vaccine or the depletion of CD4+T cells did not significantly erode the number or function of these CD8+T cells during the 15-week period. From these results, we concluded that heterologous plasmid DNA prime-adenovirus boost vaccination generated a stable pool of functional protective long-lived CD8+T cells with an effector memory phenotype.

Published
2011

14. Heterologous Plasmid DNA Prime-Recombinant Human Adenovirus 5 Boost Vaccination Generates a Stable Pool of Protective Long-Lived CD8+T Effector Memory Cells Specific for a Human Parasite, Trypanosoma cruzi

Author

Rigato, Paula Ordonhez, de Alencar, Bruna C., de Vasconcelos, José Ronnie C., Dominguez, Mariana R., Araújo, Adriano F., Machado, Alexandre V., Gazzinelli, Ricardo T., Bruna-Romero, Oscar, and Rodrigues, Mauricio M.

Abstract

ABSTRACTRecently, we described a heterologous prime-boost strategy using plasmid DNA followed by replication-defective human recombinant adenovirus type 5 as a powerful strategy to elicit long-lived CD8+T-cell-mediated protective immunity against experimental systemic infection of mice with a human intracellular protozoan parasite, Trypanosoma cruzi. In the present study, we further characterized the protective long-lived CD8+T cells. We compared several functional and phenotypic aspects of specific CD8+T cells present 14 or 98 days after the last immunizing dose and found the following: (i) the numbers of specific cells were similar, as determined by multimer staining or by determining the number of gamma interferon (IFN-γ)-secreting cells by enzyme-linked immunospot (ELISPOT) assay; (ii) these cells were equally cytotoxic in vivo; (iii) following in vitrostimulation, a slight decline in the frequency of multifunctional cells (CD107a+IFN-γ+or CD107a+IFN-γ+tumor necrosis factor alpha positive [TNF-α+]) was paralleled by a significant increase of CD107a singly positive cells after 98 days; (iv) the expression of several surface markers was identical, except for the reexpression of CD127 after 98 days; (v) the use of genetically deficient mice revealed a role for interleukin-12 (IL-12)/IL-23, but not IFN-γ, in the maintenance of these memory cells; and (vi) subsequent immunizations with an unrelated virus or a plasmid vaccine or the depletion of CD4+T cells did not significantly erode the number or function of these CD8+T cells during the 15-week period. From these results, we concluded that heterologous plasmid DNA prime-adenovirus boost vaccination generated a stable pool of functional protective long-lived CD8+T cells with an effector memory phenotype.

Published
2011
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15. Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi.

Author

Rigato PO, de Alencar BC, de Vasconcelos JR, Dominguez MR, Araújo AF, Machado AV, Gazzinelli RT, Bruna-Romero O, and Rodrigues MM

Subjects
Adenoviridae genetics, Animals, Cell Separation, Female, Flow Cytometry, Genetic Vectors, Immunization, Secondary methods, Mice, Mice, Inbred C57BL, Plasmids immunology, Vaccines, DNA immunology, Vaccines, Synthetic immunology, CD8-Positive T-Lymphocytes immunology, Chagas Disease immunology, Chagas Disease prevention & control, Immunologic Memory immunology, Protozoan Vaccines immunology, Trypanosoma cruzi immunology, Vaccination methods
Abstract

Recently, we described a heterologous prime-boost strategy using plasmid DNA followed by replication-defective human recombinant adenovirus type 5 as a powerful strategy to elicit long-lived CD8(+) T-cell-mediated protective immunity against experimental systemic infection of mice with a human intracellular protozoan parasite, Trypanosoma cruzi. In the present study, we further characterized the protective long-lived CD8(+) T cells. We compared several functional and phenotypic aspects of specific CD8(+) T cells present 14 or 98 days after the last immunizing dose and found the following: (i) the numbers of specific cells were similar, as determined by multimer staining or by determining the number of gamma interferon (IFN-γ)-secreting cells by enzyme-linked immunospot (ELISPOT) assay; (ii) these cells were equally cytotoxic in vivo; (iii) following in vitro stimulation, a slight decline in the frequency of multifunctional cells (CD107a(+) IFN-γ(+) or CD107a(+) IFN-γ(+) tumor necrosis factor alpha positive [TNF-α(+)]) was paralleled by a significant increase of CD107a singly positive cells after 98 days; (iv) the expression of several surface markers was identical, except for the reexpression of CD127 after 98 days; (v) the use of genetically deficient mice revealed a role for interleukin-12 (IL-12)/IL-23, but not IFN-γ, in the maintenance of these memory cells; and (vi) subsequent immunizations with an unrelated virus or a plasmid vaccine or the depletion of CD4(+) T cells did not significantly erode the number or function of these CD8(+) T cells during the 15-week period. From these results, we concluded that heterologous plasmid DNA prime-adenovirus boost vaccination generated a stable pool of functional protective long-lived CD8(+) T cells with an effector memory phenotype.

Published
2011
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