1. Determination of bactericidal activity against 3HC-2-Tre-labelled Mycobacterium abscessus (Mycobacteroides abscessus) by automated fluorescence microscopy.
- Author
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Mann L, Siersleben F, Lang M, and Richter A
- Subjects
- Amikacin pharmacology, Rifabutin pharmacology, Diarylquinolines pharmacology, Mycobacterium Infections, Nontuberculous microbiology, Clarithromycin pharmacology, Moxifloxacin pharmacology, Mycobacterium abscessus drug effects, Anti-Bacterial Agents pharmacology, Microbial Sensitivity Tests methods, Microscopy, Fluorescence methods
- Abstract
The minimum bactericidal concentration (MBC) of antibiotics is an important parameter for the potency of a drug in eradicating a bacterium as well as an important measure of the potential of a drug candidate in research and development. We have established a fluorescence-based microscopy method for the determination of MBCs against the non-tuberculous mycobacterium Mycobacterium abscessus (Mycobacteroides abscessus) to simplify and accelerate the performance of MBC determination compared to counting colony forming units on agar. Bacteria are labelled with the trehalose-coupled dye 3HC-2-Tre and analysed in a 96-well plate. The results of the new method are consistent with MBC determination by plating on agar. The method was used to evaluate the bactericidality of the antibiotics rifabutin, moxifloxacin, amikacin, clarithromycin and bedaquiline. Bactericidal effects against M. abscessus were observed, which are consistent with literature data., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Adrian Richter reports financial support was provided by German Research Foundation. Adrian Richter reports financial support was provided by Deutsche Mukoviszidose Stiftung. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)
- Published
- 2024
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