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5. Lactobacillus stress protein GroEL prevents colonic inflammation

Author

Dias, Alexandre M. M., Douhard, Romain, Hermetet, François, Regimbeau, Mathilde, Lopez, Tatiana E., Gonzalez, Daniel, Masson, Sophie, Marcion, Guillaume, Chaumonnot, Killian, Uyanik, Burhan, Causse, Sébastien Z., Rieu, Aurélie, Hadi, Tarik, Basset, Christelle, Chluba, Johanna, Grober, Jacques, Guzzo, Jean, Neiers, Fabrice, Ortega-Deballon, Pablo, Demidov, Oleg N., Lirussi, Frédéric, and Garrido, Carmen

Published
2021
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6. Long-term intake of Lactobacillus helveticus enhances bioavailability of omega-3 fatty acids in the mouse retina

Author

Lapaquette, Pierre, primary, Terrat, Sébastien, additional, Proukhnitzky, Lil, additional, Martine, Lucy, additional, Grégoire, Stéphane, additional, Buteau, Bénédicte, additional, Cabaret, Stéphanie, additional, Rieu, Aurélie, additional, Bermúdez-Humarán, Luis G., additional, Gabrielle, Pierre-Henry, additional, Creuzot-Garcher, Catherine, additional, Berdeaux, Olivier, additional, Acar, Niyazi, additional, and Bringer, Marie-Agnès, additional

Published
2024
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10. Long-term intake of Lacticaseibacillus helveticus enhances bioavailability of omega-3 fatty acids in the mouse retina

Author

Bringer, Marie-Agnès, Lapaquette, Pierre, Terrat, Sébastien, Proukhnitzky, Lil, Martine, Lucy, Grégoire, Stéphane, Buteau, Bénédicte, Rieu, Aurélie, Bermúdez-Humarán, Luis, Gabrielle, Pierre Henry, Creuzot‐Garcher, Catherine, Berdeaux, Olivier, Acar, Niyazi, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Bibliothèques universitaires [Université de Bourgogne] (BU), Université de Bourgogne (UB), Université Bourgogne Franche-Comté [COMUE] (UBFC), Service d'Ophtalmologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Agence Nationale de la Recherche, INRAE, French 'Investissements d’Avenir' program, project ISITE-BFC, Conseil Régional de Bourgogne, Franche-Comté [PARI grant], FEDER (European Regional Development Fund) and Institut Carnot Qualiment [grant INPROBIAUS]., ANR-11-LABX-0021,Lipstic,Lipoprotéines et santé : prévention et traitement des maladies inflammatoires non vasculaires et du cancer(2011), ANR-15-IDEX-0003,BFC,ISITE ' BFC(2015), European Project: FEDER, Julien, Sabine, Laboratoires d'excellence - Lipoprotéines et santé : prévention et traitement des maladies inflammatoires non vasculaires et du cancer - - Lipstic2011 - ANR-11-LABX-0021 - LABX - VALID, ISITE ' BFC - - BFC2015 - ANR-15-IDEX-0003 - IDEX - VALID, and FEDER - FEDER - INCOMING

Subjects
Microbiota, Probiotic, Retina, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, Plasma, Docosahexaenoic acid, Metabolism, Liver, [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs, 22 Fatty acids, Lactic acid bacteria, [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Phospholipids
Abstract

Omega-3 (n-3) polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA), are required for the structure and function of the retina. They could also help to prevent or delay the development of retinopathies. Given the accumulating evidence showing the role of gut microbiota in regulating retinal physiology and host lipid metabolism, we evaluated the potential of long-term dietary supplementation with the Gram-positive bacterium Lacticaseibacillus helveticus strain VEL12193 to modulate the retinal n-3 PUFA content. A set of complementary approaches was used to study the impact of such a supplementation on the gut microbiota and host lipid/fatty acid (FA) metabolism. L. helveticus-supplementation was associated with a decrease in retinal saturated FAs (SFAs) and monounsaturated FAs (MUFAs) as well as an increase in retinal n-3 and omega-6 (n-6) PUFAs. Interestingly, supplementation with L. helveticus enriched the retina in C22:5n-3 (docosapentaenoic acid, DPA), C22:6n-3 (DHA), C18:2n-6 (linoleic acid, LA) and C20:3n-6 (dihomo gamma-linolenic acid, DGLA). Long-term consumption of L. helveticus also modulated gut microbiota composition and some changes in OTUs abundance correlated with the retinal FA content. This study provides a proof of concept that targeting the gut microbiota could be an effective strategy to modulate the retinal FA content, including that of protective n-3 PUFAs, thus opening paths for the design of novel preventive and/or therapeutical strategies for retinopathies.

Published
2023

11. Immune-Mediated Rippling Muscle Disease Associated With Thymoma and Anti-MURC/Cavin-4 Autoantibodies

Author

da Silva Barreira, David, Laurent, Julie, Lourenço, Jessica, Novion Ducassou, Julia, Couté, Yohann, Guzzo, Jean, Rieu, Aurélie, Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Bourgogne Franche-Comté [COMUE] (UBFC), Etude de la dynamique des protéomes (EDyP), Laboratoire Biosciences et bioingénierie pour la santé (BGE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

Objectives Rippling muscle disease (RMD) is characterized by muscle stiffness, muscle hypertrophy, and rippling muscle induced by stretching or percussion. Hereditary RMD is due to sequence variants in the CAV3 and PTRF/CAVIN1 genes encoding Caveolin-3 or Cavin-1, respectively; a few series of patients with acquired autoimmune forms of RMD (iRMD) associated with AChR antibody–positive myasthenia gravis and/or thymoma have also been described. Recently, MURC/caveolae-associated protein 4 (Cavin-4) autoantibody was identified in 8 of 10 patients without thymoma, highlighting its potential both as a biomarker and as a triggering agent of this pathology. Here, we report the case of a patient with iRMD-AchR antibody negative associated with thymoma. Methods We suspected a paraneoplastic origin and investigated the presence of specific autoantibodies targeting muscle antigens through a combination of Western blotting and affinity purification coupled with mass spectrometry–based proteomic approaches. Results We identified circulating MURC/Cavin-4 autoantibodies and found strong similarities between histologic features of the patient's muscle and those commonly reported in caveolinopathies. Strikingly, MURC/Cavin-4 autoantibody titer strongly decreased after tumor resection and immunotherapy correlating with complete disappearance of the rippling phenotype and full patient remission. Discussion MURC/Cavin-4 autoantibodies may play a pathogenic role in paraneoplastic iRMD associated with thymoma.

Published
2022

12. Spontaneous Prophage Induction Contributes to the Production of Membrane Vesicles by the Gram-Positive Bacterium Lacticaseibacillus casei BL23

Author

da Silva Barreira, David, Lapaquette, Pierre, Novion Ducassou, Julia, Couté, Yohann, Guzzo, Jean, Rieu, Aurélie, Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Etude de la dynamique des protéomes (EDyP), Laboratoire Biosciences et bioingénierie pour la santé (BGE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Laboratoire de Biologie à Grande Échelle (BGE - UMR S1038), Etude de la dynamique des protéomes (EDyP ), and ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017)

Subjects
[SDV]Life Sciences [q-bio]
Abstract

The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions. This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.

Published
2022

14. Inhibition of mitophagy drives macrophage activation and antibacterial defense during sepsis

Author

Patoli, Danish, primary, Mignotte, Franck, additional, Deckert, Valérie, additional, Dusuel, Alois, additional, Dumont, Adélie, additional, Rieu, Aurélie, additional, Jalil, Antoine, additional, Van Dongen, Kevin, additional, Bourgeois, Thibaut, additional, Gautier, Thomas, additional, Magnani, Charlène, additional, Le Guern, Naig, additional, Mandard, Stéphane, additional, Bastin, Jean, additional, Djouadi, Fatima, additional, Schaeffer, Christine, additional, Guillaumot, Nina, additional, Narce, Michel, additional, Nguyen, Maxime, additional, Guy, Julien, additional, Dargent, Auguste, additional, Quenot, Jean-Pierre, additional, Rialland, Mickaël, additional, Masson, David, additional, Auwerx, Johan, additional, Lagrost, Laurent, additional, and Thomas, Charles, additional

Published
2020
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17. Tyrosine-containing peptides are precursors of tyramine produced by Lactobacillus plantarum strain IR BL0076 isolated from wine

Author

Bonnin-Jusserand Maryse, Grandvalet Cosette, Rieu Aurélie, Weidmann Stéphanie, and Alexandre Hervé

Subjects
Tyramine, Peptides, Lactobacillus plantarum, Wine, Microbiology, QR1-502
Abstract

Abstract Background Biogenic amines are molecules with allergenic properties. They are found in fermented products and are synthesized by lactic acid bacteria through the decarboxylation of amino acids present in the food matrix. The concentration of biogenic amines in fermented foodstuffs is influenced by many environmental factors, and in particular, biogenic amine accumulation depends on the quantity of available precursors. Enological practices which lead to an enrichment in nitrogen compounds therefore favor biogenic amine production in wine. Free amino acids are the only known precursors for the synthesis of biogenic amines, and no direct link has previously been demonstrated between the use of peptides by lactic acid bacteria and biogenic amine synthesis. Results Here we demonstrate for the first time that a Lactobacillus plantarum strain isolated from a red wine can produce the biogenic amine tyramine from peptides containing tyrosine. In our conditions, most of the tyramine was produced during the late exponential growth phase, coinciding with the expression of the tyrDC and tyrP genes. The DNA sequences of tyrDC and tyrP in this strain share 98% identity with those in Lactobacillus brevis consistent with horizontal gene transfer from L. brevis to L. plantarum. Conclusion Peptides amino acids are precursors of biogenic amines for Lactobacillus plantarum strain IR BL0076.

Published
2012
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18. Modulation of autophagy by probiotic bacteria: selecting and engineering strains able to stimulate autophagy in intestinal epithelial cells

Author

Al Azzaz, Jana, Rieu, Aurélie, Proukhnitzky, Lil, Bermudez Humaran, Luis, Langella, Philippe, DALLE, Frédéric, Acar, Niyazi, Guzzo, Jean, Bringer, Marie-Agnès, Lapaquette, Pierre, ProdInra, Migration, Inconnu, Procédés Alimentaires et Microbiologiques (PAM), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Université Bourgogne Franche-Comté [COMUE] (UBFC), and Projet Institut Carnot Qualiment InProbiAuS

Subjects
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2018

20. Resveratrol-Induced Xenophagy Promotes Intracellular Bacteria Clearance in Intestinal Epithelial Cells and Macrophages

Author

Al Azzaz, Jana, primary, Rieu, Aurélie, additional, Aires, Virginie, additional, Delmas, Dominique, additional, Chluba, Johanna, additional, Winckler, Pascale, additional, Bringer, Marie-Agnès, additional, Lamarche, Jérémy, additional, Vervandier-Fasseur, Dominique, additional, Dalle, Frédéric, additional, Lapaquette, Pierre, additional, and Guzzo, Jean, additional

Published
2019
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21. Probiotic Lactobacillus Strains Activities on Autophagy and Inflammatory Response

Author

Al-Azzaz, Jana, Rieu, Aurélie, Aoudia, Nabil, Ducreux, Amandine, Jego, Gaëtan, Garrido, Carmen, DALLE, FREDERIC, Lapaquette, Pierre, Guzzo, Jean, Procédés Alimentaires et Microbiologiques (PAM), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Laboratoire de parasitologie mycologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Procédés Alimentaires et Microbiologiques ( PAM ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), and Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon )

Subjects
Gastroenterology & Hepatology, viruses, education, [ SDV.MHEP.HEG ] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience; The aim of the study was to evaluate the effects of probiotic strains metabolites on the reproduction of herpes simplex virus type-1 (HSV1).

Published
2016

23. The biofilm mode of life boosts the anti-inflammatory properties of L actobacillus

Author

Rieu, Aurélie, Aoudia, Nabil, Jego, Gaëtan, Chluba, Johanna, Yousfi, Nadhir, Briandet, Romain, Deschamps, Julien, Gasquet, Benoît, Monedero, Vicente, Garrido, Carmen, Guzzo, Jean, and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV]Life Sciences [q-bio]
Published
2014

26. Differential expression of the agr locus of Listeria monocytogenes EGD-e

Author

Rieu, Aurélie, Weidmann, Stéphanie, Garmyn, Dominique, Piveteau, Pascal, Guzzo, Jean, ProdInra, Migration, Microbiologie, Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), and Université de Bourgogne (UB)

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, ComputingMilieux_MISCELLANEOUS, differential expression, agr locus of Listeria monocytogenes EGD-e, soil
Abstract

International audience

Published
2007

28. Inactivation of a small heat shock protein affects cell morphology and membrane fluidity in Lactobacillus plantarum WCFS1.

Author

UCL - SST/ISV - Institut des sciences de la vie, Capozzi, Vittorio, Weidmann, Stéphanie, Fiocco, Daniela, Rieu, Aurélie, Hols, Pascal, Guzzo, Jean, Spano, Giuseppe, UCL - SST/ISV - Institut des sciences de la vie, Capozzi, Vittorio, Weidmann, Stéphanie, Fiocco, Daniela, Rieu, Aurélie, Hols, Pascal, Guzzo, Jean, and Spano, Giuseppe

Abstract

A small heat shock gene of Lactobacillus plantarum strain WCFS1 was deleted using a Cre-lox based system. Compared to the wild type, the ∆hsp 18.55 mutant strain displayed a similar growth rate when cultivated either under optimal temperature or under different stress conditions such as heat, low pH and salt stress. However, a longer lag phase was observed when the ∆hsp 18.55 mutant strain was cultivated under short intense heat stress (50 °C). This suggests that the hsp 18.55 gene of L. plantarum may be involved in recovery of L. plantarum stressed cells in the early stage of high temperature stress. In addition, morphology of the mutant cells, investigated by scanning electron microscopy, revealed that cells clumped together and had rough surfaces, and that some of the cells had a shrunken empty appearance, which clearly contrasted with the characteristic rod-shaped, smooth-surface morphology of control L. plantarum cells. Furthermore, inactivation of the hsp 18.55 gene affected membrane fluidity and physicochemical surface properties of L. plantarum WCFS1.

Published
2011

29. Characterization of the CtsR stress response regulon in Lactobacillus plantarum.

Author

UCL - SC/BIOL - Département de biologie, Fiocco, Daniela, Capozzi, Vittorio, Collins, Michael, Gallone, Anna, Hols, Pascal, Guzzo, Jean, Weidmann, Stephanie, Rieu, Aurélie, Msadek, Tarek, Spano, Giuseppe, UCL - SC/BIOL - Département de biologie, Fiocco, Daniela, Capozzi, Vittorio, Collins, Michael, Gallone, Anna, Hols, Pascal, Guzzo, Jean, Weidmann, Stephanie, Rieu, Aurélie, Msadek, Tarek, and Spano, Giuseppe

Abstract

Lactobacillus plantarum ctsR was characterized. ctsR was found to be cotranscribed with clpC and induced in response to various abiotic stresses. ctsR deletion conferred a heat-sensitive phenotype with peculiar cell morphological features. The transcriptional pattern of putative CtsR regulon genes was examined in the DeltactsR mutant. Direct CtsR-dependent regulation was demonstrated by DNA-binding assays using recombinant CtsR and the promoters of ctsR-clpC operon and hsp1.

Published
2010

34. Characteristics and frequency of detection of fecalListeria monocytogenesshed by livestock, wildlife, and humans

Author

Lyautey, Emilie, primary, Hartmann, Alain, additional, Pagotto, Franco, additional, Tyler, Kevin, additional, Lapen, David R., additional, Wilkes, Graham, additional, Piveteau, Pascal, additional, Rieu, Aurélie, additional, Robertson, William J., additional, Medeiros, Diane T., additional, Edge, Thomas A., additional, Gannon, Victor, additional, and Topp, Edward, additional

Published
2007
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36. Distribution and Characteristics of Listeria monocytogenes Isolates from Surface Waters of the South Nation River Watershed, Ontario, Canada

Author

Lyautey, Emilie, primary, Lapen, David R., additional, Wilkes, Graham, additional, McCleary, Katherine, additional, Pagotto, Franco, additional, Tyler, Kevin, additional, Hartmann, Alain, additional, Piveteau, Pascal, additional, Rieu, Aurélie, additional, Robertson, William J., additional, Medeiros, Diane T., additional, Edge, Thomas A., additional, Gannon, Victor, additional, and Topp, Edward, additional

Published
2007
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37. Interactions in dual species biofilms between Listeria monocytogenes EGD-e and several strains of Staphylococcus aureus

Author

Rieu, Aurélie, Lemaître, Jean-Paul, Guzzo, Jean, and Piveteau, Pascal

Subjects
*BIOFILMS, *LISTERIA monocytogenes, *STAPHYLOCOCCUS aureus, *MICROBIAL aggregation, *FOOD microbiology, *LISTERIA, *FOODBORNE diseases, *SCANNING electron microscopy, *PROTEINASES
Abstract

Abstract: Six environmental isolates of Staphylococcus aureus and one collection strain were investigated for their ability to form monospecies biofilms and dual species biofilms with Listeria monocytogenes EGD-e on stainless steel coupons. All isolates were able to grow as biofilms but their ability to form monospecies biofilms differed. The population of L. monocytogenes EGD-e in dual species biofilms was not affected by the presence of S. aureus isolates except for strain CIP 53.156. The effect of L. monocytogenes EGD-e on the population of S. aureus was strain dependent: S. aureus population either increased or decreased or was not affected in the presence of L. monocytogenes EGD-e in dual species biofilms. Dual species biofilms were grown with L. monocytogenes EGD-e and the strain CIP 53.156 of S. aureus on stainless steel coupons under batch and dynamic conditions. Higher sessile populations of L. monocytogenes EGD-e were observed in the presence of S. aureus CIP 53.156. Microscope observations by scanning electron microscopy (SEM) revealed an intimate association of L. monocytogenes EGD-e and S. aureus CIP 53.156 in dual species biofilms. An increase of the number of L. monocytogenes EGD-e cells was observed in the presence of S. aureus CIP 53.156 cell-free supernatant. This activity was retained after ultrafiltation (<3 kDa), was heat stable but was lost after proteinase K treatment. [Copyright &y& Elsevier]

Published
2008
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38. Characteristics and frequency of detection of fecal Listeria monocytogenes shed by livestock, wildlife, and humans.

Author

Lyautey, Emilie, Hartmann, Alain, Pagotto, Franco, Tyler, Kevin, Lapen, David R., Wilkes, Graham, Piveteau, Pascal, Rieu, Aurélie, Robertson, William J., Medeiros, Diane T., Edge, Thomas A., Gannon, Victor, and Topp, Edward

Subjects
LISTERIA monocytogenes, DOMESTIC animals, ANIMALS, INTRACELLULAR pathogens, AMINOGLYCOSIDES, RIFAMPIN, PHENOTYPES
Abstract

Copyright of Canadian Journal of Microbiology is the property of Canadian Science Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2007
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39. Spontaneous Prophage Induction Contributes to the Production of Membrane Vesicles by the Gram-Positive Bacterium Lacticaseibacillus casei BL23.

Author

da Silva Barreira D, Lapaquette P, Novion Ducassou J, Couté Y, Guzzo J, and Rieu A

Subjects
Virus Activation, Prophages genetics, N-Acetylmuramoyl-L-alanine Amidase, Anti-Bacterial Agents pharmacology, Mitomycins, beta-Lactams, Peptidoglycan, Lacticaseibacillus casei genetics
Abstract

The formation of membrane vesicles (MVs) by Gram-positive bacteria has gained increasing attention over the last decade. Recently, models of vesicle formation have been proposed and involve the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases and the inhibition of PG synthesis by β-lactam antibiotics. The impact of these mechanisms on vesicle formation is largely dependent on the strain and growth conditions. To date, no information on the production of vesicles by the lactobacilli family has been reported. Here, we aimed to characterize the MVs released by the Gram-positive bacteria Lacticaseibacillus casei BL23 and also investigated the mechanisms involved in vesicle formation. Using electron microscopy, we established that the size of the majority of L. casei BL23 vesicles ranged from 50 to 100 nm. Furthermore, we showed that the vesicles were released consistently throughout the growth of the bacteria in standard culture conditions. The protein composition of the vesicles released in the supernatant was identified and a significant number of prophage proteins was detected. Moreover, using a mutant strain harboring a defective PLE2 prophage, we were able to show that the spontaneous and mitomycin-triggered induction of the prophage PLE2 contribute to the production of MVs by L. casei BL23. Finally, we also demonstrated the influence of prophages on the membrane integrity of bacteria. Overall, our results suggest a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress. IMPORTANCE The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions. This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis. In particular, vesicles produced by probiotic strains have been shown to play a significant role in their beneficial effects. Thus, the study of vesicle biogenesis is a key element for promoting and improving their release. Overall, our results suggest a key role of spontaneous and mitomycin-triggered prophage induction in MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23. This phenomenon is of great interest as prophage-induced MVs could potentially influence bacterial behavior, stress resistance, and vesicle functions.

Published
2022
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40. The biofilm mode of life boosts the anti-inflammatory properties of Lactobacillus.

Author

Rieu A, Aoudia N, Jego G, Chluba J, Yousfi N, Briandet R, Deschamps J, Gasquet B, Monedero V, Garrido C, and Guzzo J

Subjects
Animals, Anti-Inflammatory Agents metabolism, Cell Line, Chaperonin 60 metabolism, Gastrointestinal Tract microbiology, Humans, Immune Tolerance, Lacticaseibacillus casei metabolism, Larva microbiology, Macrophages immunology, Tumor Necrosis Factor-alpha metabolism, Bacterial Adhesion, Biofilms growth & development, Lacticaseibacillus casei immunology, Lacticaseibacillus casei physiology, Zebrafish immunology, Zebrafish microbiology
Abstract

The predominant form of life for microorganisms in their natural habitats is the biofilm mode of growth. The adherence and colonization of probiotic bacteria are considered as essential factors for their immunoregulatory function in the host. Here, we show that Lactobacillus casei ATCC334 adheres to and colonizes the gut of zebrafish larvae. The abundance of pro-inflammatory cytokines and the recruitment of macrophages were low when inflammation was induced in probiotic-fed animals, suggesting that these bacteria have anti-inflammatory properties. We treated human macrophage-differentiated monocytic THP-1 cells with supernatants of L. casei ATCC334 grown in either biofilm or planktonic cultures. TNF-α production was suppressed and the NF-κB pathway was inhibited only in the presence of supernatants from biofilms. We identified GroEL as the biofilm supernatant compound responsible, at least partially, for this anti-inflammatory effect. Gradual immunodepletion of GroEL demonstrated that the abundance of GroEL and TNF-α were inversely correlated. We confirmed that biofilm development in other Lactobacillus species affects the immune response. The biofilms supernatants of these species also contained large amounts of GroEL. Thus, our results demonstrate that the biofilm enhances the immunomodulatory effects of Lactobacillus sp. and that secreted GroEL is involved in this beneficial effect., (© 2014 John Wiley & Sons Ltd.)

Published
2014
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