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3,815 results on '"Riestra A"'

1. Air contamination, syringe contamination, and cross-contamination when using an automatic compounding device for sensitizing drugs

Author

Sessink Paul, Cajaraville Gerardo, Tamés Maria José, Riestra Ana, Alcorta Andrea, Telleria Naiara, and Grisaleña Jaione

Subjects
antibiotics, automation, drug carry-over, filling syringes, meropenem, piperacillin, Therapeutics. Pharmacology, RM1-950, Pharmaceutical industry, HD9665-9675
Abstract

To measure cross-contamination between batches of different sensitizing drugs, contamination on the outside of compounded syringes, and drug concentrations in environmental air when using an automated compounding device.

Published
2023
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2. Trichomonas vaginalis extracellular vesicles up-regulate and directly transfer adherence factors promoting host cell colonization.

Author

Kochanowsky, Joshua, Mira, Portia, Elikaee, Samira, Muratore, Katherine, Rai, Anand, Riestra, Angelica, and Johnson, Patricia

Subjects
Trichomonas vaginalis, dSTORM, extracellular vesicles, host:pathogen interactions, Extracellular Vesicles, Trichomonas vaginalis, Humans, Host-Parasite Interactions, Up-Regulation, Cell Adhesion, Female, Protozoan Proteins
Abstract

Trichomonas vaginalis, a common sexually transmitted parasite that colonizes the human urogenital tract, secretes extracellular vesicles (TvEVs) that are taken up by human cells and are speculated to be taken up by parasites as well. While the crosstalk between TvEVs and human cells has led to insight into host:parasite interactions, roles for TvEVs in infection have largely been one-sided, with little known about the effect of TvEV uptake by T. vaginalis. Approximately 11% of infections are found to be coinfections of multiple T. vaginalis strains. Clinical isolates often differ in their adherence to and cytolysis of host cells, underscoring the importance of understanding the effects of TvEV uptake within the parasite population. To address this question, our lab tested the ability of a less adherent strain of T. vaginalis, G3, to take up fluorescently labeled TvEVs derived from both itself (G3-EVs) and TvEVs from a more adherent strain of the parasite (B7RC2-EVs). Here, we showed that TvEVs generated from the more adherent strain are internalized more efficiently compared to the less adherent strain. Additionally, preincubation of G3 parasites with B7RC2-EVs increases parasite aggregation and adherence to host cells. Transcriptomics revealed that TvEVs up-regulate expression of predicted parasite membrane proteins and identified an adherence factor, heteropolysaccharide binding protein (HPB2). Finally, using comparative proteomics and superresolution microscopy, we demonstrated direct transfer of an adherence factor, cadherin-like protein, from TvEVs to the recipient parasites surface. This work identifies TvEVs as a mediator of parasite:parasite communication that may impact pathogenesis during mixed infections.

Published
2024

3. Multi-parametric atlas of the pre-metastatic liver for prediction of metastatic outcome in early-stage pancreatic cancer

Author

Bojmar, Linda, Zambirinis, Constantinos P., Hernandez, Jonathan M., Chakraborty, Jayasree, Shaashua, Lee, Kim, Junbum, Johnson, Kofi Ennu, Hanna, Samer, Askan, Gokce, Burman, Jonas, Ravichandran, Hiranmayi, Zheng, Jian, Jolissaint, Joshua S., Srouji, Rami, Song, Yi, Choubey, Ankur, Kim, Han Sang, Cioffi, Michele, van Beek, Elke, Sigel, Carlie, Jessurun, Jose, Velasco Riestra, Paulina, Blomstrand, Hakon, Jönsson, Carolin, Jönsson, Anette, Lauritzen, Pernille, Buehring, Weston, Ararso, Yonathan, Hernandez, Dylanne, Vinagolu-Baur, Jessica P., Friedman, Madison, Glidden, Caroline, Firmenich, Laetitia, Lieberman, Grace, Mejia, Dianna L., Nasar, Naaz, Mutvei, Anders P., Paul, Doru M., Bram, Yaron, Costa-Silva, Bruno, Basturk, Olca, Boudreau, Nancy, Zhang, Haiying, Matei, Irina R., Hoshino, Ayuko, Kelsen, David, Sagi, Irit, Scherz, Avigdor, Scherz-Shouval, Ruth, Yarden, Yosef, Oren, Moshe, Egeblad, Mikala, Lewis, Jason S., Keshari, Kayvan, Grandgenett, Paul M., Hollingsworth, Michael A., Rajasekhar, Vinagolu K., Healey, John H., Björnsson, Bergthor, Simeone, Diane M., Tuveson, David A., Iacobuzio-Donahue, Christine A., Bromberg, Jaqueline, Vincent, C. Theresa, O’Reilly, Eileen M., DeMatteo, Ronald P., Balachandran, Vinod P., D’Angelica, Michael I., Kingham, T. Peter, Allen, Peter J., Simpson, Amber L., Elemento, Olivier, Sandström, Per, Schwartz, Robert E., Jarnagin, William R., and Lyden, David

Published
2024
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4. Quantum states resembling classical periodic trajectories in mesoscopic elliptic billiards

Author

Riestra, Jesus G. and Gutierrez-Vega, Julio C.

Subjects
Nonlinear Sciences - Chaotic Dynamics, Mathematics - Dynamical Systems, Physics - Classical Physics, Quantum Physics
Abstract

A quantum wave function with localization on classical periodic orbits in a mesoscopic elliptic billiard has been achieved by appropriately superposing nearly degenerate eigenstates expressed as products of Mathieu functions. We analyze and discuss the rotational and librational regimes of motion in the elliptic billiard. Simplified line equations corresponding to the classical trajectories can be extracted from the quantum state as an integral equation involving angular Mathieu functions. The phase factors appearing in the integrals are connected to the classical initial positions and velocity components. We analyze the probability current density, the phase maps, and the vortex distributions of the periodic orbit quantum states for both rotational and librational motions; furthermore, they may represent traveling and standing trajectories inside the elliptic billiard., Comment: 12 pages, 12 figures

Published
2023
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7. Early career Latinas in STEM: Challenges and solutions

Author

Werner Washburne, Maggie, Trejo, JoAnn, Zambrana, Ruth Enid, Zavala, Maria Elena, Martinic, Alice, Riestra, Angelica, Delgado, Tracie, Edwards, Staci, Escobar, Thelma, Jamison-McClung, Denneal, Vazquez, Mariel, Vera, Iset, Guerra, Michelle, Marinez, Diana I, Gonzalez, Elma, and Rodriguez, Raymond L

Subjects
Biological Sciences, Biomedical and Clinical Sciences, Minority Health, Stem Cell Research, Women's Health, Health Disparities, Clinical Research, Gender Equality, Female, Humans, Hispanic or Latino, United States, Science, Medical and Health Sciences, Developmental Biology, Biological sciences, Biomedical and clinical sciences
Abstract

Mexican, Puerto Rican, and Central American Ancestry (MPRCA) individuals represent 82% of US Latinos. An intergenerational group of MPRCA women and allies met to discuss persistent underrepresentation of MPRCA women in STEM, identifying multi-level challenges and solutions. Implementation of these solutions is important and will benefit MPRCA women and the entire academic community.

Published
2023

8. Ethics-testing an eating disorder recovery memoir: a pre-publication experiment

Author

Emily T. Troscianko, Rocío Riestra-Camacho, and James Carney

Subjects
Psychiatry, RC435-571
Abstract

Abstract Background Narratives (including memoirs and novels) about eating disorders (EDs) are typically published with the intention to benefit readers, but survey evidence suggests that reading such narratives with an active ED may more often be harmful than helpful. To reduce the probability of inadvertent harm and learn more about how narrative reading and EDs interact, a pre-publication study was designed to determine whether or not a recovery memoir should be published. Methods 64 participants with a self-reported ED read either the experimental text (The Hungry Anorexic [HA]) or a control text (Ten Zen Questions [TZ]) over a roughly two-week period. All participants completed the Eating Disorder Examination Questionnaire (EDE-Q) and the Anorexia Nervosa Stages of Change Questionnaire (ANSOCQ) one week before and two weeks after reading, and answered three recurring open-ended questions at regular timepoints during and after the reading. Computational analysis of the free-text responses assessed text/response similarity and response characteristics on emotional, sensory, and action-effector dimensions. Both rating-scale and free-text data were analysed using mixed ANOVAs to test for effects of time and condition, and the university ethics board was notified in advance of the quantitative threshold for harmful effects that would prohibit the ED memoir from being published. Results On the two quantitative measures, there was an effect of time but not of condition: Significant improvement was found in both groups on the EDE-Q (with a medium-to-large effect size) and the ANSOCQ (with a very large effect size). In an ANCOVA analysis, no significant mediating effects were found for age, education, duration of professional support for the ED, or pre/post-reading BMI change. For the free-text responses, linguistic similarity measures indicated that HA responses most closely matched the text of HA, with the same being true for TZ. In a word-norm analysis, text condition significantly affected six emotional, sensory, and action-effector variables (interoception, olfaction, gustatory, mouth, torso, and hand/arm), mean scores for all of which were higher in HA responses than TZ responses. Close reading of readers’ responses explored two potential mechanisms for the positive effects of time but not condition: engagement with the during-reading prompts as part of the experimental setup and engagement with the texts’ dialogical form. Conclusions The ED memoir was found not to yield measurably harmful effects for readers with an ED, and will therefore be published. The finding that significant improvement on both quantitative measures was observed irrespective of text condition suggests that positive effects may be attributable to linguistic characteristics shared by the two texts or to elements of the reading and/or reflective processes scaffolded by both. The quantitative results and the free-text testimony have implications for our understanding of bibliotherapy, “triggering”, and the practicalities of responsible publishing.

Published
2024
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9. A Retrospective External Validation of the Cleveland Clinic Malignancy Probability Prediction Model for Indeterminate Pulmonary Nodules

Author

Michal M. Reid, MD, Jack J. Amja, MD, Irene T. Riestra Guiance, MD, Rupesh R. Andani, MBBS, Robert A. Vierkant, MS, Amit Goyal, MD, and Janani S. Reisenauer, MD

Subjects
Medicine (General), R5-920
Abstract

Objective: To perform a retrospective, multicenter, external validation of the Cleveland Clinic malignancy probability prediction model for incidental pulmonary nodules. Patients and Methods: From July 1, 2022, to May 31, 2023, we identified 296 patients who underwent tissue acquisition at Mayo Clinic (MC) (n=198) and Loyola University Medical Center (n=98) with histopathology indicating malignant (n=195) or benign (n=101). Data was collected at initial radiographic identification (point 1) and at the time of intervention (point 2). Point 3 represented the most recent data. The areas under the receiver operating characteristics were calculated for each model per time point. Calibration was evaluated by comparing the predicted and observed rates of malignancy. Results: The areas under the receiver operating characteristics at time points 1, 2, and 3 for the MC model were 0.67 (95% CI, 0.61-0.74), 0.67 (95% CI, 0.58-0.77), and 0.70 (95% CI, 0.63-0.76), respectively. The Cleveland Clinic model (CCM) was 0.68 (95% CI, 0.61-0.74), 0.75 (95% CI, 0.65-0.84), and 0.72 (95% CI, 0.66-0.78), respectively. The mean ± SD estimated probability for malignant pulmonary nodules (PNs) at time points 1, 2, and 3 for the CCM was 64.2±25.9, 65.8±24.0, and 64.7±24.4, which resembled the overall proportion of malignant PNs (66%). The mean estimated probability of malignancy for the MC model at each time point was 38.3±27.4, 36.2±24.4, and 42.1±27.3, substantially lower than the observed proportion of malignancies. Conclusion: The CCM found discrimination similar to its internal validation and good calibration. The CCM can be used to augment clinical and shared decision-making when evaluating high-risk PNs.

Published
2024
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11. CD74 supports accumulation and function of regulatory T cells in tumors

Author

Bonnin, Elisa, Rodrigo Riestra, Maria, Marziali, Federico, Mena Osuna, Rafael, Denizeau, Jordan, Maurin, Mathieu, Saez, Juan Jose, Jouve, Mabel, Bonté, Pierre-Emmanuel, Richer, Wilfrid, Nevo, Fabien, Lemoine, Sebastien, Girard, Nicolas, Lefevre, Marine, Borcoman, Edith, Vincent-Salomon, Anne, Baulande, Sylvain, Moreau, Helene D., Sedlik, Christine, Hivroz, Claire, Lennon-Duménil, Ana-Maria, Tosello Boari, Jimena, and Piaggio, Eliane

Published
2024
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12. XMM-Newton

Author

Schartel, Norbert, González-Riestra, Rosario, Kretschmar, Peter, Kirsch, Marcus, Rodríguez-Pascual, Pedro, Rosen, Simon, Santos-Lleó, Maria, Smith, Michael, Stuhlinger, Martin, and Verdugo-Rodrigo, Eva

Subjects
Astrophysics - Instrumentation and Methods for Astrophysics, Astrophysics - High Energy Astrophysical Phenomena
Abstract

The X-ray Multi-mirror Mission (XMM-Newton) provides simultaneous non-dispersive spectroscopic X-ray imaging and timing, medium resolution dispersive X-ray spectroscopy and optical/UV imaging, spectroscopy and timing. In combination, the imaging cameras offer an effective area over the energy range from 150 eV to 12 keV of up to 2500 cm$^2$ at 1.5 keV and $\sim$1800 cm$^2$ at 5 keV. The gratings cover an energy range from 0.4 keV to 2.2 keV with a combined effective area of up to 120 cm$^2$ at 0.8 keV. XMM-Newton offers unique opportunities for a wide variety of sensitive X-ray observations accompanied by simultaneous optical/UV measurements. The majority of XMM-Newton's observing time is made available to the astronomical community by peer-reviewed Announcements of Opportunity. The scientific exploitation of XMM-Newton data is aided by an observatory-class X-ray facility which provides analysis software, pipeline processing, calibration and catalogue generation. Around 380 refereed papers based on XMM-Newton data are published each year with a high fraction of papers reporting transformative scientific results., Comment: 37 pages, 28 figures, Invited chapter for {\it Handbook of X-ray and Gamma-ray Astrophysics} (Eds. C. Bambi and A. Santangelo, Springer Singapore, expected in 2022

Published
2022
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13. Communication in the ICU: An Unintended Nocebo Effect?

Author

Irene Riestra Guiance MD, Lindsey Wallace DScPAS, PA-C, Katalin Varga PhD, DSc, Alexander Niven MD, Megan Hosey PhD, Jillene Chitulangoma PT, DPT, Kemuel Philbrick MD, Ognjen Gajic MD, Madeline Weiman RN, Emily Schmitt OT, David Pasko RRT, and Lioudmila Karnatovskaia MD

Subjects
Medicine (General), R5-920
Abstract

To identify medical phrases utilized by the critical care team that may have an unintended impact on the critically ill patient, we administered an anonymous survey to multi-professional critical care team members. We elicited examples of imprecise language that may have a negative emotional impact on the critically ill. Of the 1600 providers surveyed, 265 offered 1379 examples (912 unique) which were clustered into 5 categories. Medical jargon (eg, “riding the vent”) was most prevalent (n = 549). There were 217 negative suggestions (eg, “you will feel a stick and a burn”). Hyperboles (eg, “black cloud”) were common (n = 198) while homonyms (ie “he fibbed”) accounted for 150 examples. Phrases such as “code brown in there” were categorized as metonyms (n = 144). 121 metaphors/similes (eg, “rearranging deck chairs on the Titanic”) were provided. Phrases that have the potential to negatively impact critically ill patient perceptions are commonplace in critical care practice. Whether these everyday communication habits lead to an unintended nocebo effect on mental health outcomes of the critically ill deserves further study.

Published
2024
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15. XMM-Newton

Author

Schartel, Norbert, González-Riestra, Rosario, Kretschmar, Peter, Kirsch, Marcus, Rodríguez-Pascual, Pedro, Rosen, Simon, Santos-Lleó, Maria, Smith, Michael, Stuhlinger, Martin, Verdugo-Rodrigo, Eva, Bambi, Cosimo, editor, and Santangelo, Andrea, editor

Published
2024
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18. Association of microbiological factors with mortality in Escherichia coli bacteraemia presenting with sepsis/septic shock: a prospective cohort study

Author

Maldonado, Natalia, López-Hernández, Inmaculada, López-Cortés, Luis Eduardo, Martínez Pérez-Crespo, Pedro María, Retamar-Gentil, Pilar, García-Montaner, Andrea, De la Rosa Riestra, Sandra, Sousa-Domínguez, Adrián, Goikoetxea, Josune, Pulido-Navazo, Ángeles, Del Valle Ortíz, María, Natera-Kindelán, Clara, Jover-Sáenz, Alfredo, Arco-Jiménez, Alfonso del, Armiñanzas-Castillo, Carlos, Aller-García, Ana Isabel, Fernández-Suárez, Jonathan, Marrodán-Ciordia, Teresa, Boix-Palop, Lucía, Smithson-Amat, Alejandro, Reguera-Iglesias, José María, Galán-Sánchez, Fátima, Bahamonde, Alberto, Sánchez-Calvo, Juan Manuel, Gea-Lázaro, Isabel, Pérez-Camacho, Inés, Reyes-Bertos, Armando, Becerril-Carral, Berta, Pascual, Álvaro, and Rodríguez-Baño, Jesús

Published
2024
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19. An Analysis of Class-as-Race and Gender Ideology in the US Young Adult Sports Novel 'Racing Savannah' (2013)

Author

Riestra-Camacho, Rocío

Abstract

Equine fiction is an established genre in the English juvenile literary canon. Current works in the field appeal to adolescent readers thanks to their interface between classic motifs of vintage and contemporary forms of equine narratives. Performing a close reading of selected passages in Miranda Kenneally's "Racing Savannah" (2013), this paper acknowledges how this novel is a revitalization and a challenge to this pattern. Savannah, who is more gifted than her companions, is subordinate to the decisions of the junior of the household where she works. Jack Goodwin, the protagonist's romantic lead, educated in a neocolonialist background of male jockeying, becomes Savannah's marker of difference according to her sex and lower socioeconomic status, which lay at the root of her later racialization despite her being a white character. My analysis attempts to expose how these difficulties encountered by the protagonist to become a professional jockey articulate past and present constraints of the horse-racing ladder.

Published
2020

20. Human Autosomal Recessive DNA Polymerase Delta 3 Deficiency Presenting as Omenn Syndrome

Author

Riestra, Maria Rodrigo, Pillay, Bethany A., Willemsen, Mathijs, Kienapfel, Verena, Ehlers, Lisa, Delafontaine, Selket, Pinton, Antoine, Wouters, Marjon, Hombrouck, Anneleen, Sauer, Kate, Bossuyt, Xavier, Voet, Arnout, Soenen, Stefaan J., Conde, Cecilia Dominguez, Bucciol, Giorgia, Boztug, Kaan, Humblet-Baron, Stephanie, Touzart, Aurore, Rieux-Laucat, Frédéric, Notarangelo, Luigi D., Moens, Leen, and Meyts, Isabelle

Published
2024
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22. EMBRACE-WATERS statement: Recommendations for reporting of studies on antimicrobial resistance in wastewater and related aquatic environments

Author

Hassoun-Kheir, Nasreen, Stabholz, Yoav, Kreft, Jan-Ulrich, de la Cruz, Roberto, Dechesne, Arnaud, Smets, Barth F, Romalde, Jesús L, Lema, Alberto, Balboa, Sabela, García-Riestra, Carlos, Torres-Sangiao, Eva, Neuberger, Ami, Graham, David, Quintela-Baluja, Marcos, Stekel, Dov J, Graham, Jay, Pruden, Amy, Nesme, Joseph, Sørensen, Søren Johannes, Hough, Rupert, and Paul, Mical

Subjects
Antimicrobial resistance, Reporting, Recommendations, Aquatic, Environment, One health, Medical Microbiology
Abstract

BackgroundA One Health approach requires integrative research to elucidate antimicrobial resistance (AMR) in the environment and the risks it poses to human health. Research on this topic involves experts from diverse backgrounds and professions. Shortcomings exist in terms of consistent, complete, and transparent reporting in many environmental studies. Standardized reporting will improve the quality of scientific papers, enable meta-analyses and enhance the communication among different experts. In this study, we aimed to generate a consensus of reporting standards for AMR research in wastewater and related aquatic environments.MethodsBased on a risk of bias assessment of the literature in a systematic review, we proposed a set of study quality indicators. We then used a multistep modified Delphi consensus to develop the EMBRACE-WATERS statement (rEporting antiMicroBial ResistAnCE in WATERS), a checklist of recommendations for reporting in studies of AMR in wastewater and related aquatic environments.FindingsConsensus was achieved among a multidisciplinary panel of twenty-one experts in three steps. The developed EMBRACE-WATERS statement incorporates 21 items. Each item contains essential elements of high-quality reporting and is followed by an explanation of their rationale and a reporting-example. The EMBRACE-WATERS statement is primarily intended to be used by investigators to ensure transparent and comprehensive reporting of their studies. It can also guide peer-reviewers and editors in evaluation of manuscripts on AMR in the aquatic environment. This statement is not intended to be used to guide investigators on the methodology of their research.InterpretationWe are hopeful that this statement will improve the reporting quality of future studies of AMR in wastewater and related aquatic environments. Its uptake would generate a common language to be used among researchers from different disciplines, thus advancing the One Health approach towards understanding AMR spread across aquatic environments. Similar initiatives are needed in other areas of One Health research.

Published
2021

29. CIELO-RGS: a catalogue of soft X-ray ionized emission lines

Author

Mao, Junjie, Kaastra, J. S., Guainazzi, M., Gonzalez-Riestra, R., Santos-LLeo, M., Kretschmar, P., Grinberg, V., Kalfountzou, E., Ibarra, A., Matzeu, G., Parker, M., and Rodriguez-Pascual, P.

Subjects
Astrophysics - High Energy Astrophysical Phenomena
Abstract

High-resolution X-ray spectroscopy has advanced our understanding of the hot Universe by revealing physical properties like kinematics, temperature, and abundances of the astrophysical plasmas. Despite the technical and scientific achievements, the lack of scientific products at a level higher than count spectra is hampering full scientific exploitation of high-quality data. This paper introduces the Catalogue of Ionized Emission Lines Observed by the Reflection Grating Spectrometer (CIELO-RGS) onboard the XMM-Newton space observatory. The CIELO-RGS catalogue aims to facilitate the exploitation of emission features in the public RGS spectra archive, in particular, to perform the correlation between X-ray spectral diagnostics parameters with measurements at other wavelengths. This paper focuses on the methodology of catalogue generation, describing the automated line detection algorithm. A moderate sample (~2400 observations) of high-quality RGS spectra available at XMM-Newton Science Archive is used as our starting point. A list of potential emission lines is selected based on a multi-scale peak detection algorithm in a uniform and automated way without prior assumption on the underlying astrophysical model. The candidate line list is validated via spectral fitting with simple continuum and line profile models. We also compare the catalogue content with published literature results on a small number of exemplary sources. We generate a catalogue of emission lines ~12000 detected in ~1600 observations toward stars, X-ray binaries, supernovae remnants, active galactic nuclei, and groups and clusters of galaxies. For each line, we report the observed wavelength, broadening, energy and photon flux, and equivalent width, etc., Comment: accepted to A&A

Published
2019
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30. Tuning the Innate Immune Response to Cyclic Dinucleotides by Using Atomic Mutagenesis

Author

Li, Yao, Fin, Andrea, Rovira, Alexander R, Su, Yichi, Dippel, Andrew B, Valderrama, Jonathan Andrés, Riestra, Angelica M, Nizet, Victor, Hammond, Ming C, and Tor, Yitzhak

Subjects
HIV/AIDS, Cyclic GMP, Immunity, Innate, Interferons, Nucleotides, Cyclic, Signal Transduction, cyclic dinucleotides, innate immune response, modified nucleosides, STING agonists, Medicinal and Biomolecular Chemistry, Biochemistry and Cell Biology, Organic Chemistry
Abstract

Cyclic dinucleotides (CDNs) trigger the innate immune response in eukaryotic cells through the stimulator of interferon genes (STING) signaling pathway. To decipher this complex cellular process, a better correlation between structure and downstream function is required. Herein, we report the design and immunostimulatory effect of a novel group of c-di-GMP analogues. By employing an "atomic mutagenesis" strategy, changing one atom at a time, a class of gradually modified CDNs was prepared. These c-di-GMP analogues induce type-I interferon (IFN) production, with some being more potent than c-di-GMP, their native archetype. This study demonstrates that CDN analogues bearing modified nucleobases are able to tune the innate immune response in eukaryotic cells.

Published
2020

31. Siglec-14 Enhances NLRP3-Inflammasome Activation in Macrophages

Author

Tsai, Chih-Ming, Riestra, Angelica M, Ali, Syed Raza, Fong, Jerry J, Liu, Janet Z, Hughes, Gillian, Varki, Ajit, and Nizet, Victor

Subjects
Biodefense, Vaccine Related, Emerging Infectious Diseases, Prevention, Clinical Research, 2.1 Biological and endogenous factors, Aetiology, Inflammatory and immune system, Infection, Humans, Inflammasomes, Lectins, Macrophages, NLR Family, Pyrin Domain-Containing 3 Protein, Receptors, Cell Surface, Streptococcal Infections, Streptococcus agalactiae, THP-1 Cells, Siglec, Interleukin-1 beta, Caspase-1, Inflammasome, Innate immunity, Vimentin, Interleukin-1β, Medical and Health Sciences
Abstract

Pathogenic microorganisms are sensed by the inflammasome, resulting in the release of the pro-immune and proinflammatory cytokine interleukin-1β (IL-1β). In humans, the paired sialic acid-binding Ig-like lectin receptors Siglec-5 (inhibitory) and Siglec-14 (activating) have been shown to have reciprocal roles in regulating macrophage immune responses, but their interaction with IL-1β signaling and the inflammasome has not been characterized. Here we show that in response to known inflammasome activators (ATP, nigericin) or the sialic acid-expressing human bacterial pathogen group B Streptococcus (GBS), the presence of Siglec-14 enhances, whereas Siglec-5 reduces, inflammasome activation and macrophage IL-1β release. Human THP-1 macrophages stably transfected with Siglec-14 exhibited increased caspase-1 activation, IL-1β release and pyroptosis after GBS infection, in a manner blocked by a specific inhibitor of nucleotide-binding domain leucine-rich repeat protein 3 (NLRP3), a protein involved in inflammasome assembly. Another leading pathogen, Streptococcus pneumoniae, lacks sialic acid but rather prominently expresses a sialidase, which cleaves sialic acid from macrophages, eliminating cis- interactions with the lectin receptor, thus attenuating Siglec-14 induced IL-1β secretion. Vimentin, a cytoskeletal protein released during macrophage inflammatory activation is known to induce the inflammasome. We found that vimentin has increased interaction with Siglec-14 compared to Siglec-5, and this interaction heightened IL-1β production by Siglec-14-expressing cells. Siglec-14 is absent from some humans because of a SIGLEC5/14 fusion polymorphism, and we found increased IL-1β expression in primary macrophages from SIGLEC14+/+ individuals compared to those with the SIGLEC14-/+ and SIGLEC14-/- genotypes. Collectively, our results identify a new immunoregulatory role of Siglec-14 as a positive regulator of NLRP3 inflammasome activation.

Published
2020

34. I-CARE, a European Prospective Cohort Study Assessing Safety and Effectiveness of Biologics in Inflammatory Bowel Disease

Author

Gornet, Jean-Marc, Beaugerie, Laurent, Shaji, Sebastian, Peyrin-Biroulet, Laurent, Reimund, Jean-Marie, Hebuterne, Xavier, Amiot, Aurélien, Armelao, Franco, Blanc, Pierre, Papi, Claudio, De Chambrun, Guillaume Pineton, Roblin, Xavier, Chu, Karmiris, Konstantinos, Shariq, Sohail, Viazis, Nikolaos, Limdi, Jimmy, Eder H, Piotr, Michalopoulos, Georgios, Bell, Andrew, Biancone, Livia, Dewitte, Marie, Mazhar, Zia, Franchimont, Denis, Nancey, Stephane, Macaigne, Gilles, Principi, Maria Beatrice, Fumery, Mathurin, Parkes, Gareth, Valats, Jean-Christophe, Doherty, Glen, Bouguen, Guillaume, Molnar, Tamás, Tsai, Hersin, Gangi, Mohsin, Pedersen, Natalia, Heluwaert, Frédéric, Shenderey, Richard, Zeissig, Sebastian, Butterworth, Jeffrey, Castiglione, Fabiana, Corless, Lynsey, Zallot, Camille, Baert, Filip, Singh, Salil, Sonwalkar, Sunil, Clayton, Elizabeth, Rahier, Jean-François, Vani, Deven, Bellaiche, Guy, De Vos, Martine, Kirchgesner, Julien, Kopylov, Uri, Lobaton, Triana, Locher, Christophe, Mantzaris, Gerassimos, Abouda, George, Smith, Katie, Sprakes, Michael, Theodoropoulou, Angeliki, Wesley, Emma, Bonnet, Joëlle, Elphick, David, Gilletta, Cyrielle, Gordon, John, Laharie, David, Nakad, Antoine, Orlando, Ambrogio, Dubois, Patrick, Hasselblatt, Peter, Michiels, Christophe, Preston, Cathryn, Staicu, Anca, Vuitton, Lucine, Kaassis, Mehdi, Speight, Ally, Ghosh, Deb, Löwenberg, Mark, Mathieu, Nicolas, Pelletier, Anne-Laure, Phillips, Anne, Magro, Fernando, Altwegg, Romain, Avni, Irit, biron, Landy, Jonathon, Nachury, Maria, Shenoy, Achuth, Trang, Caroline, Abitbol, Vered, Bamias, Georgios, Farkas, Klaudia, Maaser, Christian, Shitrit, Ariella, Siegmund, Britta, Filippi, Jérôme, O'morain, Colm, Yanai, Henit, Costes, Laurent, Hobday, David, Szepes, Zoltán, Calabrese, Emma, Dallal, Helen, Fung, Michael, Ramadas, Arvind, Baburajan, Bijay, Koss, Konrad, Barberis, Christophe, Buisson, Anthony, Amil, Morgane, Balestrieri, Paola, Johnson, Matthew, Tzouvala, Maria, Viennot, Stéphanie, Nagy, Ferenc, Thompson, Nick, Alric, Laurent, Samuel, Sunil, Bourrier, Anne, Chanteloup, Elise, Del Tedesco, Emilie, Harbord, Marcus, Lobo, Alan, Myers, Sally, Pollok, Richard, Ahmad, Tariq, Chaudhary, Rakesh, Karakoidas, Christos, Soliman, Ashraf, Stefanescu, Carmen, Theocharis, Georgios, Branden, Stijn Vanden, Beltran, Belén, Bouhnik, Yoram, Bourreille, Arnaud, Branco, Joana, Colleypriest, Ben, Eliakim, Rami, Knight, Paul, O'toole, Aoibhlinn, Robles, Virgina, Triantafyllou, Konstantinos, Bosca, Marta Maia, Lambrecht, Guy, Mosquera, Lucia Marquez, Panter, Simon, Pappa, Aikaterini, Simon, Marion, Sivaji, Ganesh, Bellanger, Christophe, Belle, Arthur, Borruel, Natalia, Egan, Laurence, Peeters, Harald, Sharpstone, Daniel, Arasaradnam, Ramesh, Benitez, José Manuel, Dahlerup, Jens Frederik, Giouleme, Olga, Gisbert, Javier P., Helwig, Ulf, Minguez, Miguel, Tsironi, Eftychia, Variola, Angela, Allen, Patrick, Boivineau, Lucille, Cole, Andy, Dib, Nina, Gomollon, Fernando, Johnston, Richard, Katsanos, Konstantinos, Kennedy, Nick, Kiszka-Kanowitz, Marianne, Marin-Jimenez, Ignacio, Miheller, Pál, Nos, Pilar, Saraj, Othman, Vinter-Jensen, Lars, Zittan, Eran, Baudry, Clotilde, Calvet, Xavier, Cazelles-Boudier, Marie-Christine, Coenegrachts, Jean-Louis, Cullen, Garret, Daperno, Marco, Dhar, Anjan, Gerard, Romain, Jensen, Nanna, Maharshak, Nitsan, Mcalindon, Mark, Mcloughlin, Simon, Parkes, Miles, Patel, Kamal, Peixoto, Armando, Polymeros, Dimitrios, Portela, Francisco, Rocca, Rodolfo, Seksik, Philippe, Subramanian, Sreedhar, Tennenbaum, Ruth, Atreya, Raja, Bachmann, Oliver, Berger, Arthur, Bor, Renáta, Buckley, Maire, Carpio, Daniel, Chaparro, María, Costa, Francesco, Domenech, Eugeni, Esteve, Maria, Foley, Stephen, Guardiola, Jordi, Koutroubakis, Ioannis, Kuehbacher, Tanja, Landman, Cécilia, Lavagna, Alessandro, Manceñido, Noemí, Mañosa, Míriam, Martín-Arranz, Maria Dolores, Plastaras, Laurianne, Scribano, Maria Lia, Sengupta, Subhasish, Teich, Nils, Tran-Minh, My-Linh, Zampeli, Evanthia, Amininejad, Leila, Arroyo, Teresa, Attar, Alain, Backman, Ann-Sofie, Bálint, Anita, Beckly, John, Ben Horin, Shomron, Bernardo, Sónia, Caillo, Ludovic, Caron, Bénédicte, Shanika de Silva, María, FábiáN, Anna, Fiorino, Gionata, Gutierrez, Ana, Lahat, Adi, Masmoudi, Mohamed, Mendolaro, Marco, Muls, Vinciane, Poullenot, Florian, Probert, Christopher, Reenaers, Catherine, Rutka, Mariann, Sarwari, Zaman, Sayer, Joanne, Sicilia, Beatriz, Sousa, Helena, van Kemseke, Catherine, Zabana, Yamile, Astegiano, Marco, Banim, Paul, Bettenworth, Dominik, Boualit, Médina, Brodersen, Jacob Broder, Christidou, Angeliki, Cooney, Rachel, Pinto, João Cortez, Cravo, Portugal Marília, Cremer, Anneline, Danese, Silvio, di Sabatino, Antonio, Fallingborg, Jan, Ferronato, Antonio, Planella, Esther Garcia, Gupta, Sanjay, Halfvarson, Jonas, Israeli, Eran, Kestenbaum, Samantha, Larsen, Lone, Macken, Elisabeth, Mathou, Nicoletta, Milassin, Ágnes, Pofelski, Joanna, Ricci, Chiara, Rodriguez-Moranta, Francisco, Schmidt-Lauber, Martin, Shaw, Ian, Soares, Marta, Soliman, Heithem, Triantos, Christos, Zografos, Konstantinos, Agrawal, Anurag, Armuzzi, Alessandro, Aubourg, Alexandre, Acosta, Manuel Barreiro-de, Barrio, Jesús, Bergemalm, Daniel, Bermejo, Fernando, Bodini, Giorgia, Bohr, Johan, Bossuyt, Peter, Christodoulou, Dimitrios, Claessens, Christophe, Collins, Paul, de Francisco, Ruth, Garcia, Santiago, Georgopoulos, Sotirios, Goutorbe, Felix, Kalantzis, Chrisostomos, Kourikou, Anastasia, Mace, Vincent, Malamut, Georgia, Ministro, Paula, Larmurier, Isabelle Nion, Ricart, Elena, Serrero, Mélanie, Sheridan, Juliette, Weimers, Petra, Andersen, Vibeke, Arroja, Bruno, Bokemeyer, Bernd, Bujanda, Luis, Degand, Thibault, Eriksson, Carl, Garceau, Cécile, Glerup, Henning, Goren, Idan, Jackson, Lucina, Koch, Stéphane, Mesonero, Francisco, Ordas, Ingrid, Riviere, Pauline, Saibeni, Simone, Soares, João, Tavernier, Noémie, Theede, Klaus, Ungar, Bella, Bästlein, Elke, Gasbarrini, Antonio, Protopapas, Andreas, Reindl, Wolfgang, Bossa, Fabrizio, Hart, Ailsa, Heil, Franz-Josef, O'Connor, Anthony, Oldenburg, Bas, Pastorelli, Luca, Stephen patchett, Ramakrishnan, Subramaniam, de Caestecker, John, Echarri, Ana, Kevans, David, Büning, Jürgen, Coelho, Rosa, Jansen, Jeroen, Koslowski, Benjamin, Wells, Christopher, Ceballos, Daniel, König, Ingrid, Padmanabhan, Hari, Patani, Timi, Qureshi, Raheel, Zagorowicz, Edyta, Allez, Matthieu, Archavlis, Emmanouil, Bonnet, Delphine, Guidi, Luisa, Mcnamara, Deirdre, Vernia, Piero, Weidenhiller, Michael, Alon, Lang, Boysen, Trine, Delattre, Charlotte, Farrell, Richard, Krüger, Rolf-Achim, Paupard, Thierry, Vind, Ida, Caprioli, Flavio, Gancho, Vladimir, Quentin, Vincent, Avidan, Benjamin, D’Haens, Geert, Mccarthy, Jane, Snook, Jonathon, Soufleris, Konstantinos, Zerbib, Frank, Carter, Dan, Depla, Annekatrien, Eisenbach, Thomas, Fries, Walter, Grammatikos, Nikolaos, Ilegems, Saskia, Lopez-Sanroman, Antonio, Moreau, Jacques, Riegler, Gabriele, Rietdijk, Svend, Rocha, Marta, Rosa, Isabelle, Ryan, Barbara, Yeremenko, Yelena, Boruchowicz, Arnaud, Damião, Filipe, Laoudi, Foteini, Lügering, Andreas, Macarri, Giampiero, Thomopoulos, Konstantinos, Barros, Luísa, Blixt, Thomas, Garros, Aurélien, Khorrami, Sam, Sokol, Harry, Sturm, Andreas, Livovsky, Dan, Maul, Jochen, Miks, Heinrich, Papadopoulos, Vasileios, Schmidt, Carsten, Snir, Yifat, Svenningsen, Lise, Ahmed, Wafaa, Broitman, Yelena, Cuillerier, Emmanuel, Kant, Prashant, Leyden, Jan, Lichtenstein, Lev, Lopes, Susana, Martineau, Chloé, Mulcahy, Hugh, Schweitzer, Axel, Van Schaik, Fiona, Banai, Hagar, Danion, Pauline, Dulery, Charlotte, Fidder, Herma, Gay, Claire, Hagege, Hervé, Harnois, Florence, Jørgensen, Søren Peter, Müller-Ziehm, Jens, Oikonomou, Michail, Palmela, Carolina, Schulze/Röske, Jörg, Smith, Mark, Thurm, Tamar, Bresso, Francesca, Brixi, Hedia, Jones, John, Macmathuna, Padraig, Painchart, Claire, Ron, Yulia, Vester-Andersen, Marianne, Alexandrino, Gonçalo, Börner, Norbert, Cardoso, Mariana, Chagas, Cristina, Dignaß, Axel, Dotan, Iris, Hedin, Charlotte, Karatzas, Pantelis, Kasapidis, Panagiotis, Palatka, Károly, Sakizlis, Georgios, Wilson, Ana, Bosanko, Nick, Caldeira, Paulo, Gagniere, Charlotte, Libier, Louise, Meunier, Camille, Moog, Gero, Pasquion, Audrey, Pica, Roberta, Akbar, Ayesha, Arab, Nadia, Cadiot, Guillaume, Carvalho, João, Charpignon, Claire, Fellermann, Laus, Fishman, Sigal, Fraser, Gerald, Gluck, Nathan, Hoesl, Mark, Kierkus, Jarosław, Klopocka, Maria, Arranz, Eduardo Martin, Menchen, Luis, Nikolaus, Susanna, Petrache, Anca, Ponsioen, Cyriel, Riestra, Sabino, Robledo, Pilar, Rodriguez, Cristina, Samer, Misheal, Tischer, Matthias, Wypych, Joanna, Baudon, Julien, Bezzio, Cristina, Boschetti, Gilles, Burisch, Johan, Creed, Tom, Demarzo, Maria Giulia, Festa, Stefano, Figueroa, Andrés, Julsgaard, Mette, Navarro, Pablo, Perez-Galindo, Pablo, Rouillon, Cléa, Sablich, Emanuele, Tosca, Joan, Vidon, Mathias, Vidon, Marine, Vitte, René-Louis, Wampach, Anne, Baumann, Cédric, Urmes, Isabelle Clerc, Rousseau, Hélène, Borie, Marc, Uzzan, Mathieu, Chatten, Kelly, Peter, Rimmer, Tariq, Iqbal, Cossignani, Marta, Cañete, Fiorella, Holvoet, Tom, Krasz, Susanne, Dias, Sandra, Abalia, Hadas, Abaza, Aziza, Abramovich, Gal, Ackzell, Ingrid, Adams, Carol, Addleton, Catherine, Alfambra, Erika, Algaba, Alicia, Allcock, Clare, Allison, Joanna, Amouriaux, Karine, Anderson, Julie, Anderson, Emma, Appelmans, Saskia, Armstrong, Lisa, Atkins, Stacey, Attaran-Bandarabadi, Masoumeh, Bailey, Yvonne, Bardot, Stephanie, Beck, Natasha, Bennett, Lillie, Bergfeld, Jonathan Phil, Berkane, Ramdane, Boey, Hanne, Bowlas, Louise, Bradley-Potts, Joanne, Brear, Tracy, Bretlander-Peters, Nicole, Brown, Ellen, Brown, Johanna, Buckingham, Elizabeth, Buellens, Katrien, Bull, Rhian, Burke, Maura, Burns, Leighanne, Burton, Julie, Bwalya, Agness, Cabanas, Karine, Callaghan, Muriel, Camou, Océane, Campbell, Debbie, Capoferro, Elvira, Carnahan, Mandy, Carnio, Cornelia, Carter, Anne, Clack, Concetta Casali, Chedouba, Leïla, Cipriano, Bessie, Claeys, Sophie, Closset, Manon, Coban, Dilek, Cococcia, Sara, Coe, Carolann, Cole, Helen, Collet, Emilie, Collins, Kayleigh, Combes, Isabelle, Connor, Emma, Constantin, Kathryn, Cooke, Susan, Cornet, Nathanaëlle, Corrihons, Estelle, Corsino, Pilar, Cortaville, Rosie, Cotterill, Donna, Cowton, Amanda, Cox, Harriet, Cripps, Viktoria, Crowder, Amanda, Cukier, Tzufit, Daniel, Amelia, Dawe, Chris, de Haan, Jose, Croix, Rosanna de la, Dejonckheere, Evva, Villanegro, Juan Delare, Delaval, Guillaume, Delliponti, Mariangela, Delommez, Aude, Detry, Emilie, Dhanaratne, Melanie, Galan, Laura Diez, Dodel, Marie, Dooks, Emma, Du Cheyron, Joseph, Duane, Linda, Vulgo Cochran, Jennifer Dulling, Dyer, Simona, Dymond, Harvey, Ekblad, Charlotte, Elliott, Kerry, Emmerson, Ingrid, Eugene-Jolchine, Irène, Fleming, Lorna, Fletcher, Eve, Ford, Sarah, Forshaw, Greg, Foulds, Angela, Francois, Caroline, Fuge, Nicole, Gafni, Gal, Ganon, Miri, Nuñez, Olga Garcia, Ramirez, Laura Garcia, Gelder, Sophie, Gettkowski, Raimonda, Gilardi, Daniela, Giuffrida, Paolo, Gobert, Vincent, Godden, Jo, Godwin, Nuala, Goulden, Kay, Graham, Sharon, Green, Charlotte, Green, Marie, Gueye, Aboubakar, Guler, Tuba, Gustavsson, Ida, Hadjisavvas, Helena, Hammonds, Fiona, Hantzi, Christina, Hauke, Marion, Haydock, Julie, Hayes, Orla, Nislev, Lizette Helbo, Hochstodter, Jessica, Hogg, Ashleigh, Hölbing, Manuela, Holland, Maureen, Holsbergen, Maartje, Howard, Linda, Hoyda, Aviya, Hull, Robert, Irish, Jane, Jackson, Wendy, Janssen, Wendy, Jeffrey, Lesley, Jourdan, Sofia, Jutrowska, Izabela, Kaniel, Chava, Karezos, Theofilos, Kelly, Niamh, Kelly, Jessica, Kennedy, Mary, Kennedy, Una, Kibaru, Joyce, Kirkman, Gemma, Klaproth, Janine, Kneese, Corinna, Koch, Andrea, Kokke, Kathleen, Koppelow, Martha, Krause, Sabine, Krauspe, Sabine, Kwakkenbos, Petra, Labarile, Nunzia, Lang, Hannah, Lassailly, Marianne, Leconte, Martine, Lepczynski, Linda, Levell, Emma, Levhar, Nina, Lindhort, Kerstin, Lisle, Jessica, Cauce, Beatriz Lopez, Lorenz, Gabriele, Lovati, Ambra, Lowry, Tracey, Lund, Margareta, Vorderbrügge, Anne Lutz, Maansson, Suzanne, Madapathage, Videsheka, Cheviakoff, Maelys, Magness, Alison, Manley, Orla, Manyoni, Catherine, Marg, Ingke, Marra, Antonella, Martins, Carole, Massella, Arianna, Mathias, Aurore, Mervyn, Danielle, Minsart, Charlotte, Mitchell, Sally, Monks, Kathleen, Montero, Mélanie, Moore, Alson, Moser, Maren, Moss, Alison, Mullen, Angela, Murciano, M. Francisca, Naylor, Deanna, Nehus, Ansgar, Nicholson, Anne, Nöding, Sarah, Nolan, Sinead, Nörenberg, Janet, Northcott, Clare, O'Connell, Jim, O’Kelly, Alison, Orbach-Zingboim, Noam, Orobitg, Judit, Otieno, Charlene, Owen, Charlotte, Patch, Sarah, Pauker, Maor, Pauli, Renate, Pearson, Harriet, Peggy, Falgon, Petit, Séverine, Petrissans, Christine, Piergallini, Simona, Pippard, Lucy, Pitt, Laura, Pócsik, Gabriella, Poher, Yoann, Pomes, Chloé, Pritchard, Lucy, Puchades, Laura, Quaid, Sheena, Rana, Aleem, Raynard, Dana, Reilly, Mykla, Reinert, Sonja, Reinknecht, Manuela, Renner, Baerbel, Reynolds, Rob, Rizzuto, Giulia, Robinson, Matthew, Robrechts, Joke, Rodriguez, Eva M., Rosenblum, Efrat, Russel, Tamlyn, Sadare, Ibiyemi, Salama, Noa, Schakel, Toos, Schauer, Anja, Schiavoni, Elisa, Shaw, Caroline, Shelton, Sarah, Sicart, Virginie, Siouville, Elodie, Smith, Orla, Soude, Théo, Stephenson, Sophie, Stephenson, Elaine, Steppe, Marjan, Sterkx, An, Stickley, Jo, Sugrue, Kathleen, Swietec, Natalia, Tasiaux, Charlotte, Thamu, Bhavneet, Thomas, Susane, Tobi, Ogwa, Touabi, Kahina, Tovi, Shifra, Tregonning, Julie, Turchini, Laura, Unkhoff, Julia, Unruh, Olesya, Uzun, Nurcan, Van Aert, Frauke, Bergh, Sandrine Vanden, Vandenbroucke, Louise, Vansteenkiste, Laura, Vardit, Shay, Vergriete, Valentin, Walker, Elaine, Warner, Eleanor, Watchorn, Olivia, Watson, Ekaterina, Wauthier, Marie-Claire, Weetman, Belgium Maria, Weston, Margaret, West-Petroschka, Wiebke, Wienecke, Susann, Wierling, Kerstin, Wiestler, Miriam, Wilcox, Rebecca, Wilhelmsen, Elva, Williams, Angharad, Williamson, Georgina, Wilson, Deborah, Wistance, Kate, Wortmann, Nicolas, Wurie, Subie, Yadgar, Karin, Young, Gail, Young, Megan, Aucouturier, Julien, Bertin, Marie- Jo, Bougrine, Hasnae, Coisnon, Marie, Defrance, Antoine, Gutierrez, Kati, Harouz, Amel, Jerber, Laure, Khlifi, Aida, Kirati, Amina, Liworo, Nasaladjine, Logoltat, Maude, Mailhat, Charlotte, M'Bayi, Chancely, Medane, Yasmina, Merkhoufa, Dalal, Elhad, Saouda Mohamed, Monthe, Bertille, Moyon, Fanny, Rabiega, Pascaline, Sekela, Jennifer, Thilloy, Charlotte, Hamamouche, Naima, Partisotti, Frederic, Blandin, Patrick, Mokhtari, Hocine, Coutard, Laure, and Doherty, Glen A.

Published
2023
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38. Clinical Characteristics and Outcomes of Patients With SARS-CoV-2 Reinfection

Author

Isin Yagmur Comba, MD, Irene Riestra Guiance, MD, Cristina Corsini Campioli, MD, Douglas Challener, MD, MS, Priya Sampathkumar, MD, Robert Orenstein, DO, Joel Gordon, MD, Wendelyn Bosch, MD, and John C. O’Horo, MD, MPH

Subjects
Medicine (General), R5-920
Abstract

Objective: To examine the clinical characteristics, risk of hospitalization, and mortality of patients diagnosed with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reinfection. Patients and Methods: We retrospectively reviewed all patients with SARS-CoV-2 reinfection at all Mayo Clinic sites between May 23, 2020, and June 30, 2021 (the period before the emergence of the Delta variant in the United States). The reinfection was defined as a positive SARS-CoV-2 test more than or equal to 90 days after initial infection or 45-89 days after with symptomatic second episode. Vaccination status was classified as fully vaccinated, first dose, and unvaccinated. Comparative analysis of baseline characteristics and comorbidities was performed by hospitalization and vaccination status. The survival analysis of the hospitalized patients was performed using Cox proportional hazard regression. Results: Among the 554 patients reinfected with SARS-CoV-2, 59 (10.6%) were pediatric, and 495 (89.4%) were adults. The median age was 13.9 years (interquartile range, 8.5-16.5 years) for the pediatric and 50.2 years (interquartile range, 28.4-65.6 years) for the adult population. Among the adult patients, the majority were unvaccinated (83.4%, n=413), and the duration to reinfection from initial infection was the longest in the fully vaccinated group (P

Published
2022
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40. POS0378 HETEROZYGOUS LOSS-OF-FUNCTION RELA MUTATIONS LEAD TO SYSTEMIC LUPUS ERYTHEMATOSUS OR AUTOINFLAMMATORY SYNDROME

Author

Riller, Q., primary, Barnabei, L., additional, Rodrigo-Riestra, M., additional, Stolzenberg, M. C., additional, Pelle, O., additional, Delage, L., additional, Becquard, T., additional, Courteille, C., additional, Marchais, M., additional, De Cevins, C., additional, Brunaud, C., additional, Magerus, A., additional, Luka, M., additional, Sorin, B., additional, Boussard, C., additional, Salomon, R., additional, Ménager, M., additional, Hié, M., additional, Neven, B., additional, Baud, V., additional, Skokowa, J., additional, Sogkas, G., additional, Jamilloux, Y., additional, Merlin, E., additional, Belot, A., additional, Picard, C., additional, Boursier, G., additional, Riviere, E., additional, Georgin-Lavialle, S., additional, Quartier, P., additional, Bader-Meunier, B., additional, Fischer, A., additional, Miner, J. J., additional, and Rieux-Laucat, F., additional

Published
2024
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42. Ultrasound Cut-Off Values for Rectus Femoris for Detecting Sarcopenia in Patients with Nutritional Risk

Author

de Luis Roman, Daniel, primary, García Almeida, José Manuel, additional, Bellido Guerrero, Diego, additional, Guzmán Rolo, Germán, additional, Martín, Andrea, additional, Primo Martín, David, additional, García-Delgado, Yaiza, additional, Guirado-Peláez, Patricia, additional, Palmas, Fiorella, additional, Tejera Pérez, Cristina, additional, García Olivares, María, additional, Maíz Jiménez, María, additional, Bretón Lesmes, Irene, additional, Alzás Teomiro, Carlos Manuel, additional, Guardia-Baena, Juan Manuel, additional, Calles Romero, Laura A., additional, Prior-Sánchez, Inmaculada, additional, García-Luna, Pedro Pablo, additional, González Pacheco, María, additional, Martínez-Olmos, Miguel Ángel, additional, Alabadí, Blanca, additional, Alcántara-Aragón, Valeria, additional, Palma Milla, Samara, additional, Martín Folgueras, Tomás, additional, Micó García, Andrea, additional, Molina-Baena, Begoña, additional, Rendón Barragán, Henry, additional, Rodríguez de Vera Gómez, Pablo, additional, Riestra Fernández, María, additional, Jiménez Portilla, Ana, additional, López-Gómez, Juan J., additional, Pérez Martín, Nuria, additional, Montero Madrid, Natalia, additional, Zabalegui Eguinoa, Alba, additional, Porca Fernández, Cristina, additional, Tapia Guerrero, María José, additional, Ruiz Aguado, Marta, additional, Velasco Gimeno, Cristina, additional, Herrera Martínez, Aura D, additional, Novo Rodríguez, María, additional, Iglesias Hernández, Natalia C., additional, de Damas Medina, María, additional, González Navarro, Irene, additional, Vílchez López, Francisco Javier, additional, Fernández-Pombo, Antía, additional, and Olveira, Gabriel, additional

Published
2024
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43. Mortality impact of further delays in active targeted antibiotic therapy in bacteraemic patients that did not receive initial active empiric treatment: results from the prospective, multicentre cohort PROBAC

Author

Riestra, Sandra De la Rosa, primary, Pérez-Crespo, Pedro María Martínez, additional, Rodríguez, María Teresa Pérez, additional, Sousa, Adrián, additional, Goikoetxea, Josune, additional, Iglesias, José María Reguera, additional, Armiñanzas, Carlos, additional, López-Hernández, Inmaculada, additional, López-Cortés, Luis E., additional, and Rodríguez-Baño, Jesús, additional

Published
2024
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44. 94 Dominant negative IKKα and immunodeficiency with immune dysregulation

Author

Riller, Quentin, primary, Sorin, Boris, additional, Courteille, Charline, additional, Voyer, Tom Le, additional, Pellé, Olivier, additional, Stolzenberg, Marie-Claude, additional, Jeanpierre, Marie, additional, Becquard, Thomas, additional, Boussard, Charlotte, additional, Michel, Victor, additional, Rodrigo-Riestra, Maria, additional, Picard, Capucine, additional, Corneau, Aurélien, additional, Meyts, Isabelle, additional, Baud, Veronique, additional, Fischer, Alain, additional, Puel, Anne, additional, Casanova, Jean-Laurent, additional, Boulanger, Cécile, additional, Neven, Bénédicte, additional, and Rieux-Laucat, Frédéric, additional

Published
2024
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47. PHYLLOGYNY ET MISOGYNY

Author

Fernández, José García, primary, Giacobbe, Giuliana Antonella, additional, and Camacho, Rocío Riestra, additional

Published
2022
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48. A Novel Cadherin-like Protein Mediates Adherence to and Killing of Host Cells by the Parasite Trichomonas vaginalis

Author

Chen, Yi-Pei, Riestra, Angelica M, Rai, Anand Kumar, and Johnson, Patricia J

Subjects
Vector-Borne Diseases, Emerging Infectious Diseases, Vaccine Related, Infectious Diseases, 2.2 Factors relating to the physical environment, Aetiology, 2.1 Biological and endogenous factors, Infection, Cadherins, Calcium, Cell Adhesion, Cell Line, Epithelial Cells, Female, Humans, Mucous Membrane, Protein Domains, Protozoan Proteins, Transcriptional Activation, Trichomonas vaginalis, Up-Regulation, host-pathogen interactions, parasite attachment, Microbiology
Abstract

Trichomonas vaginalis, a prevalent sexually transmitted parasite, adheres to and induces cytolysis of human mucosal epithelial cells. We have characterized a hypothetical protein, TVAG_393390, with predicted tertiary structure similar to that of mammalian cadherin proteins involved in cell-cell adherence. TVAG_393390, renamed cadherin-like protein (CLP), contains a calcium-binding site at a position conserved in cadherins. CLP is surface localized, and its mRNA and protein levels are significantly upregulated upon parasite adherence to host cells. To test the roles of CLP and its calcium-binding dependency during host cell adherence, we first demonstrated that wild-type CLP (CLP) binds calcium with a high affinity, whereas the calcium-binding site mutant protein (CLP-mut) does not. CLP and CLP-mut constructs were then used to overexpress these proteins in T. vaginalis Parasites overexpressing CLP have ∼3.5-fold greater adherence to host cells than wild-type parasites, and this increased adherence is ablated by mutating the calcium-binding site. Additionally, competition with recombinant CLP decreased parasite binding to host cells. We also found that overexpression of CLP induced parasite aggregation which was further enhanced in the presence of calcium, whereas CLP-mut overexpression did not affect aggregation. Lastly, parasites overexpressing wild-type CLP induced killing of host cells ∼2.35-fold, whereas parasites overexpressing CLP-mut did not have this effect. These analyses describe the first parasitic CLP and demonstrate a role for this protein in mediating parasite-parasite and host-parasite interactions. T. vaginalis CLP may represent convergent evolution of a parasite protein that is functionally similar to the mammalian cell adhesion protein cadherin, which contributes to parasite pathogenesis.IMPORTANCE The adherence of pathogens to host cells is critical for colonization of the host and establishing infection. Here we identify a protein with no known function that is more abundant on the surface of parasites that are better at binding host cells. To interrogate a predicted function of this protein, we utilized bioinformatic protein prediction programs which allowed us to uncover the first cadherin-like protein (CLP) found in a parasite. Cadherin proteins are conserved metazoan proteins with central roles in cell-cell adhesion, development, and tissue structure maintenance. Functional characterization of this CLP from the unicellular parasite Trichomonas vaginalis demonstrated that the protein mediates both parasite-parasite and parasite-host adherence, which leads to an enhanced killing of host cells by T. vaginalis Our findings demonstrate the presence of CLPs in unicellular pathogens and identify a new host cell binding protein family in a human-infective parasite.

Published
2019

49. Trichomonas vaginalis Induces NLRP3 Inflammasome Activation and Pyroptotic Cell Death in Human Macrophages

Author

Riestra, Angelica Montenegro, Valderrama, J Andrés, Patras, Kathryn A, Booth, Sharon D, Quek, Xing Yen, Tsai, Chih-Ming, and Nizet, Victor

Subjects
Prevention, Sexually Transmitted Infections, Infectious Diseases, Vaccine Related, Biodefense, Aetiology, 2.1 Biological and endogenous factors, Infection, Inflammatory and immune system, Good Health and Well Being, Animals, Caspase 1, Humans, Inflammasomes, Interleukin-1beta, Intracellular Signaling Peptides and Proteins, Macrophages, Mice, NLR Family, Pyrin Domain-Containing 3 Protein, Phosphate-Binding Proteins, Pyroptosis, THP-1 Cells, Trichomonas vaginalis, Medical and Health Sciences
Abstract

Trichomonas vaginalis is a sexually transmitted, eukaryotic parasite that causes trichomoniasis, the most common nonviral, sexually transmitted disease in the USA and worldwide. Little is known about the molecular mechanisms involved in the host immune response to this widespread parasite. Here we report that T. vaginalis induces NLRP3 inflammasome activation in human macrophages, leading to caspase-1 activation and the processing of pro-IL-1β to the mature and bioactive form of the cytokine. Using inhibitor-based approaches, we show that NLRP3 activation by T. vaginalis involves host cell detection of extracellular ATP via P2X7 receptors and potassium efflux. In addition, our data reveal that T. vaginalis inflammasome activation induces macrophage inflammatory cell death by pyroptosis, known to occur via caspase-1 cleavage of the gasdermin D protein, which assembles to form pores in the host cell membrane. We found that T. vaginalis-induced cytolysis of macrophages is attenuated in gasdermin D knockout cells. Lastly, in a murine challenge model, we detected IL-1β production in vaginal fluids in response to T. vaginalis infection in vivo. Together, our findings mechanistically dissect how T. vaginalis contributes to the production of the proinflammatory IL-1β cytokine and uncover pyroptosis as a mechanism by which the parasite can trigger host macrophage cell death.

Published
2019

50. The murine vaginal microbiota and its perturbation by the human pathogen group B Streptococcus

Author

Vrbanac, Alison, Riestra, Angelica M, Coady, Alison, Knight, Rob, Nizet, Victor, and Patras, Kathryn A

Subjects
Microbiology, Biological Sciences, Biomedical and Clinical Sciences, Emerging Infectious Diseases, Genetics, Infectious Diseases, 2.2 Factors relating to the physical environment, Aetiology, 2.1 Biological and endogenous factors, Infection, Good Health and Well Being, Animals, Bacteria, Disease Models, Animal, Female, Humans, Mice, Mice, Inbred C57BL, Microbiota, RNA, Ribosomal, 16S, Streptococcal Infections, Streptococcus agalactiae, Vagina, Vaginal microbiome, Murine model, Estrous cycle, Group B Streptococcus, 16S rRNA sequencing, Agricultural and Veterinary Sciences, Medical and Health Sciences, Medical microbiology
Abstract

BackgroundComposition of the vaginal microbiota has significant influence on female urogenital health and control of infectious disease. Murine models are widely utilized to characterize host-pathogen interactions within the vaginal tract, however, the composition of endogenous vaginal flora remains largely undefined with modern microbiome analyses. Here, we employ 16S rRNA amplicon sequencing to establish the native microbial composition of the vaginal tract in adult C57Bl/6 J mice. We further interrogate the impact of estrous cycle and introduction of the human vaginal pathobiont, group B Streptococcus (GBS) on community state type and stability, and conversely, the impact of the vaginal microbiota on GBS persistence.ResultsSequencing analysis revealed five distinctive community states of the vaginal microbiota dominated largely by Staphylococcus and/or Enterococcus, Lactobacillus, or a mixed population. Stage of estrus did not impact microbial composition. Introduction of GBS decreased community stability at early timepoints; and in some mice, GBS became the dominant bacterium by day 21. Endogenous Staphylococcus abundance correlated with GBS ascension into the uterus, and increased community stability in GBS-challenged mice.ConclusionsThe murine vaginal flora is diverse and fluctuates independently of the estrous cycle. Endogenous flora may impact pathogen colonization and dissemination and should be considered in urogenital infection models.

Published
2018

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