1. High specificity of line-immunoassay based algorithms for recent HIV-1 infection independent of viral subtype and stage of disease
- Author
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Yerly Sabine, Shah Cyril, Martinetti Gladys, Andreutti Corinne, Steffen Ingrid, Gorgievski Meri, Bürgisser Philippe, Regenass Stephan, Bisset Leslie R, Schüpbach Jörg, Klimkait Thomas, Gebhardt Martin, Schöni-Affolter Franziska, and Rickenbach Martin
- Subjects
Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Serologic testing algorithms for recent HIV seroconversion (STARHS) provide important information for HIV surveillance. We have shown that a patient's antibody reaction in a confirmatory line immunoassay (INNO-LIATM HIV I/II Score, Innogenetics) provides information on the duration of infection. Here, we sought to further investigate the diagnostic specificity of various Inno-Lia algorithms and to identify factors affecting it. Methods Plasma samples of 714 selected patients of the Swiss HIV Cohort Study infected for longer than 12 months and representing all viral clades and stages of chronic HIV-1 infection were tested blindly by Inno-Lia and classified as either incident (up to 12 m) or older infection by 24 different algorithms. Of the total, 524 patients received HAART, 308 had HIV-1 RNA below 50 copies/mL, and 620 were infected by a HIV-1 non-B clade. Using logistic regression analysis we evaluated factors that might affect the specificity of these algorithms. Results HIV-1 RNA Conclusions The specificity of most Inno-Lia algorithms was high and not affected by HIV-1 variability, advanced disease and other factors promoting false-recent results in other STARHS. Specificity should be good in any group of untreated HIV-1 patients.
- Published
- 2011
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