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281 results on '"Richter, Vladimir A."'

3. Using Computer Modeling and Experimental Methods to Screen for Aptamers That Bind to the VV-GMCSF-LACT Virus.

Author

Dymova, Maya, Vasileva, Natalia, Malysheva, Daria, Ageenko, Alisa, Shchugoreva, Irina, Artyushenko, Polina, Tomilin, Felix, Kichkailo, Anna S., Kuligina, Elena, and Richter, Vladimir

Subjects
EPITOPES, VACCINIA, ONCOLYTIC virotherapy, VIRAL envelopes, CYTOTOXINS
Abstract

Oncolytic virotherapy is a promising approach for cancer treatment. However, when introduced into the body, the virus provokes the production of virus-neutralizing antibodies, which can reduce its antitumor effect. To shield viruses from the immune system, aptamers that can cover the membrane of the viral particle are used. Aptamers that specifically bind to the JX-594 strain of the vaccinia virus were developed earlier. However, the parameters for binding to the recombinant virus VV-GMCSF-Lact, developed based on the LIVP strain of the vaccinia virus, may differ due its different repertoire of antigenic determinants on its membrane compared to JX-594. In this work, the spatial atomic structures of aptamers to JX-594 and bifunctional aptamers were determined using molecular modeling. The efficiency of viral particles binding to the aptamers (EC50), as well as the cytotoxicity and stability of the aptamers were studied. The synergistic effect of the VV-GMCSF-Lact combination with the aptamers in the presence of serum was investigated using human glioblastoma cells. This proposed approach allowed us to conduct a preliminary screening of sequences using in silico modeling and experimental methods, and identified potential candidates that are capable of shielding VV-GMCSF-Lact from virus-neutralizing antibodies. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Diamond Processing by Focused Ion Beam - Surface Damage and Recovery

Author

Bayn, Igal, Bolker, Asaf, Cytermann, Catherine, Meyler, Boris, Richter, Vladimir, Salzman, Joseph, and Kalish, Rafi

Subjects
Physics - Optics, Condensed Matter - Mesoscale and Nanoscale Physics, Quantum Physics
Abstract

The Nitrogen Vacancy color center (NV-) in diamond is of great interest for novel photonic applications. Diamond nano-photonic structures are often implemented using Focused-Ion-Beam (FIB) processing, leaving a damaged surface which has a detrimental effect on the color center luminescence. The FIB processing effect on single crystal diamond surfaces and their photonic properties is studied by Time of Flight Secondary Ion Mass Spectrometry (TOF-SIMS) and photoluminescence (PL). Exposing the processed surface to hydrogen plasma, followed by chemical etching, drastically decreases implanted Ga concentration, resulting in a recovery of the NV- photo-emission and in a significant increase of the NV-/NV0 ratio., Comment: 12 pages, 3 figures

Published
2011
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7. Characterizing Aptamer Interaction with the Oncolytic Virus VV-GMCSF-Lact

Author

Dymova, Maya A., primary, Malysheva, Daria O., additional, Popova, Victoria K., additional, Dmitrienko, Elena V., additional, Endutkin, Anton V., additional, Drokov, Danil V., additional, Mukhanov, Vladimir S., additional, Byvakina, Arina A., additional, Kochneva, Galina V., additional, Artyushenko, Polina V., additional, Shchugoreva, Irina A., additional, Rogova, Anastasia V., additional, Tomilin, Felix N., additional, Kichkailo, Anna S., additional, Richter, Vladimir A., additional, and Kuligina, Elena V., additional

Published
2024
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10. Transcriptome Changes in Glioma Cells upon Infection with the Oncolytic Virus VV-GMCSF-Lact

Author

Semenov, Dmitriy V., primary, Vasileva, Natalia S., additional, Dymova, Maya A., additional, Mishinov, Sergey V., additional, Savinovskaya, Yulya I., additional, Ageenko, Alisa B., additional, Dome, Anton S., additional, Zinchenko, Nikita D., additional, Stepanov, Grigory A., additional, Kochneva, Galina V., additional, Richter, Vladimir A., additional, and Kuligina, Elena V., additional

Published
2023
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28. Transcriptome Changes in Glioma Cells Cultivated under Conditions of Neurosphere Formation

Author

Vasileva, Natalia S., primary, Kuligina, Elena V., additional, Dymova, Maya A., additional, Savinovskaya, Yulya I., additional, Zinchenko, Nikita D., additional, Ageenko, Alisa B., additional, Mishinov, Sergey V., additional, Dome, Anton S., additional, Stepanov, Grigory A., additional, Richter, Vladimir A., additional, and Semenov, Dmitry V., additional

Published
2022
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35. CRISPR-Cas9 mediated knockout of AnxA6 gene enhances influenza A virus replication in low-permissive HEK293FT cell line

Author

Komissarov, Andrey, primary, Sergeeva, Mariia, additional, Zhuravlev, Evgenii, additional, Medvedev, Sergey, additional, Malakhova, Anastasia, additional, Andreeva, Evgeniya, additional, Shurygina, Anna-Polina, additional, Gorshkov, Andrey, additional, Timofeeva, Mariia, additional, Balakhonova, Evgenia, additional, Grudinin, Mikhail, additional, Zakian, Suren, additional, Richter, Vladimir, additional, and Stepanov, Grigory, additional

Published
2022
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37. Aptamers Enhance Oncolytic Viruses' Antitumor Efficacy.

Author

Dymova, Maya A., Kichkailo, Anna S., Kuligina, Elena V., and Richter, Vladimir A.

Subjects
APTAMERS, NUCLEIC acids, VIROTHERAPY, IMMUNE system, OLIGONUCLEOTIDES, CANCER treatment
Abstract

Oncolytic viruses are highly promising for cancer treatment because they target and lyse tumor cells. These genetically engineered vectors introduce therapeutic or immunostimulatory genes into the tumor. However, viral therapy is not always safe and effective. Several problems are related to oncolytic viruses' targeted delivery to the tumor and immune system neutralization in the bloodstream. Cryoprotection and preventing viral particles from aggregating during storage are other critical issues. Aptamers, short RNA, or DNA oligonucleotides may help to crawl through this bottleneck. They are not immunogenic, are easily synthesized, can be chemically modified, and are not very demanding in storage conditions. It is possible to select an aptamer that specifically binds to any target cell, oncolytic virus, or molecule using the SELEX technology. This review comprehensively highlights the most important research and methodological approaches related to oncolytic viruses and nucleic acid aptamers. Here, we also analyze possible future research directions for combining these two methodologies to improve the effectiveness of cancer virotherapy. [ABSTRACT FROM AUTHOR]

Published
2023
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38. Aptamers for Addressed Boron Delivery in BNCT: Effect of Boron Cluster Attachment Site on Functional Activity.

Author

Novopashina, Darya S., Dymova, Maya A., Davydova, Anna S., Meschaninova, Mariya I., Malysheva, Daria O., Kuligina, Elena V., Richter, Vladimir A., Kolesnikov, Iaroslav A., Taskaev, Sergey Yu., and Vorobyeva, Mariya A.

Subjects
INDUCTIVELY coupled plasma atomic emission spectrometry, BORON-neutron capture therapy, APTAMERS, BORON, NEUTRON beams, POLYMERASE chain reaction, ATOMIC absorption spectroscopy
Abstract

Among the great variety of anti-cancer therapeutic strategies, boron neutron capture therapy (BNCT) represents a unique approach that doubles the targeting accuracy due to the precise positioning of a neutron beam and the addressed delivery of boron compounds. We have recently demonstrated the principal possibility of using a cell-specific 2′-F-RNA aptamer for the targeted delivery of boron clusters for BNCT. In the present study, we evaluated the amount of boron-loaded aptamer inside the cell via two independent methods: quantitative real-time polymerase chain reaction and inductive coupled plasma–atomic emission spectrometry. Both assays showed that the internalized boron level inside the cell exceeds 1 × 109 atoms/cell. We have synthesized closo-dodecaborate conjugates of 2′-F-RNA aptamers GL44 and Waz, with boron clusters attached either at the 3′- or at the 5′-end. The influence of cluster localization was evaluated in BNCT experiments on U-87 MG human glioblastoma cells and normal fibroblasts and subsequent analyses of cell viability via real-time cell monitoring and clonogenic assay. Both conjugates of GL44 aptamer provided a specific decrease in cell viability, while only the 3′-conjugate of the Waz aptamer showed the same effect. Thus, an individual adjustment of boron cluster localization is required for each aptamer. The efficacy of boron-loaded 2′-F-RNA conjugates was comparable to that of 10B-boronophenylalanine, so this type of boron delivery agent has good potential for BNCT due to such benefits as precise targeting, low toxicity and the possibility to use boron clusters made of natural, unenriched boron. [ABSTRACT FROM AUTHOR]

Published
2023
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46. The Recombinant Fragment of Human κ-Casein Induces Cell Death by Targeting the Proteins of Mitochondrial Import in Breast Cancer Cells

Author

Richter, Max, Wohlfromm, Fabian, Kähne, Thilo, Bongartz, Hannes, Seyrek, Kamil, Kit, Yuriy, Chinak, Olga, Richter, Vladimir A., Koval, Olga A., and Lavrik, Inna N.

Subjects
mitochondria, TOM70, lactaptin, breast cancer, cell death, RL2, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, lcsh:RC254-282, κ-Casein, Article
Abstract

Breast cancer is still one of the most common cancers for women. Specified therapeutics are indispensable for optimal treatment. In previous studies, it has been shown that RL2, the recombinant fragment of human &kappa, Casein, induces cell death in breast cancer cells. However, the molecular mechanisms of RL2-induced cell death remain largely unknown. In this study, mechanisms of RL2-induced cell death in breast cancer cells were systematically investigated. In particular, we demonstrate that RL2 induces loss of mitochondrial membrane potential and cellular ATP loss followed by cell death in breast cancer cells. The mass spectrometry-based screen for RL2 interaction partners identified mitochondrial import protein TOM70 as a target of RL2, which was subsequently validated. Further to this, we show that RL2 is targeted to mitochondria after internalization into the cells, where it can also be found in the dimeric form. The importance of TOM70 and RL2 interaction in RL2-induced reduction in ATP levels was validated by siRNA-induced downregulation of TOM70, resulting in the partial rescue of ATP production. Taken together, this study demonstrates that RL2&ndash, TOM70 interaction plays a key role in RL2-mediated cell death and targeting this pathway may provide new therapeutic options for treating breast cancer.

Published
2020

47. Method of Measuring High-LET Particles Dose

Author

Dymova, Maya, primary, Dmitrieva, Maria, additional, Kuligina, Elena, additional, Richter, Vladimir, additional, Savinov, Sergey, additional, Shchudlo, Ivan, additional, Sycheva, Tatiana, additional, Taskaeva, Iuliia, additional, and Taskaev, Sergey, additional

Published
2021
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