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Persistent overuse of water supplies from the Colorado River during recent decades has substantially depleted large storage reservoirs and triggered mandatory cutbacks in water use. The river holds critical importance to more than 40 million people and more than two million hectares of cropland. Therefore, a full accounting of where the river’s water goes en route to its delta is necessary. Detailed knowledge of how and where the river’s water is used can aid design of strategies and plans for bringing water use into balance with available supplies. Here we apply authoritative primary data sources and modeled crop and riparian/wetland evapotranspiration estimates to compile a water budget based on average consumptive water use during 2000–2019. Overall water consumption includes both direct human uses in the municipal, commercial, industrial, and agricultural sectors, as well as indirect water losses to reservoir evaporation and water consumed through riparian/wetland evapotranspiration. Irrigated agriculture is responsible for 74% of direct human uses and 52% of overall water consumption. Water consumed for agriculture amounts to three times all other direct uses combined. Cattle feed crops including alfalfa and other grass hays account for 46% of all direct water consumption.

While most bacterial and archaeal taxa living in surface soils remain undescribed, this problem is exacerbated in deeper soils, owing to the unique oligotrophic conditions found in the subsurface. Additionally, previous studies of soil microbiomes have focused almost exclusively on surface soils, even though the microbes living in deeper soils also play critical roles in a wide range of biogeochemical processes. We examined soils collected from 20 distinct profiles across the United States to characterize the bacterial and archaeal communities that live in subsurface soils and to determine whether there are consistent changes in soil microbial communities with depth across a wide range of soil and environmental conditions. We found that bacterial and archaeal diversity generally decreased with depth, as did the degree of similarity of microbial communities to those found in surface horizons. We observed five phyla that consistently increased in relative abundance with depth across our soil profiles: Chloroflexi, Nitrospirae, Euryarchaeota, and candidate phyla GAL15 and Dormibacteraeota (formerly AD3). Leveraging the unusually high abundance of Dormibacteraeota at depth, we assembled genomes representative of this candidate phylum and identified traits that are likely to be beneficial in low-nutrient environments, including the synthesis and storage of carbohydrates, the potential to use carbon monoxide (CO) as a supplemental energy source, and the ability to form spores. Together these attributes likely allow members of the candidate phylum Dormibacteraeota to flourish in deeper soils and provide insight into the survival and growth strategies employed by the microbes that thrive in oligotrophic soil environments.IMPORTANCE Soil profiles are rarely homogeneous. Resource availability and microbial abundances typically decrease with soil depth, but microbes found in deeper horizons are still important components of terrestrial ecosystems. By studying 20 soil profiles across the United States, we documented consistent changes in soil bacterial and archaeal communities with depth. Deeper soils harbored communities distinct from those of the more commonly studied surface horizons. Most notably, we found that the candidate phylum Dormibacteraeota (formerly AD3) was often dominant in subsurface soils, and we used genomes from uncultivated members of this group to identify why these taxa are able to thrive in such resource-limited environments. Simply digging deeper into soil can reveal a surprising number of novel microbes with unique adaptations to oligotrophic subsurface conditions.

Introduction: In the past 20 years there has been a significant decline in the number of inpatient psychiatric beds in the United States, while the number of patients seeking psychiatric treatment in the emergency department (ED) has increased over the same time period. Given the increase in demand for psychiatric services and decrease in availability of inpatient treatment the ED is becoming the de facto place of treatment for the majority of psychiatric crises. Psychiatric patients experience longer lengths of stay (LOS) when compared to non-psychiatric patients, especially when transfer to another facility is required. Therefore, improvements in the efficiency of evaluation, treatment and disposition of psychiatric patients benefit both patients and the EDs that care for them. Methods: To improve throughput and reduce wait times in the ED at our Level I trauma center located in the Upstate region of South Carolina, we implemented several improvements. We then tracked pre- and post-intervention metrics, including LOS and the time from ED consultation order to the completion of psychiatric consultant documentation. The intervention consisted of several protocols with various checkpoints for required documentation necessary for progression through overall mental health evaluation and treatment. In addition, structured psychiatrist and social worker evaluation-note templates were standardized to improve documentation accuracy, consistency, efficiency and overall patient safety. A separate tracking system is monitored by a dedicated psychiatric advanced practice provider to ensure compliance on note completion and order set utilization. The time from ED consult order to completion of psychiatric consultant documentation and mean LOS (in hours +/-standard deviation [SD]) were measured for six months before (10/2016 to 03/2017) and eight months after (4/2017 to 11/2017) institution of these protocols. We then compared pre- and post-intervention measures using Student’s t-test (p

Perineuronal nets (PNNs) are mesh‐like structures on the surfaces of parvalbumin‐expressing inhibitory and other neurons, and consist of proteoglycans such as aggrecan, brevican, and neurocan. PNNs regulate the Excitatory/Inhibitory (E/I) balance in the brain and are formed at the closure of critical periods of plasticity during development. PNN formation is disrupted in Fragile X Syndrome, which is caused by silencing of the fragile X messenger ribonucleoprotein 1 (Fmr1) gene and loss of its protein product FMRP. FXS is characterized by impaired synaptic plasticity resulting in neuronal hyperexcitability and E/I imbalance. Here, we investigate how PNN formation is altered in FXS. PNNs are reduced in Fmr1 KO mouse brain when examined by staining for the lectin Wisteria floribunda agglutin (WFA) and aggrecan. Examination of PNNs by WFA staining at P14 and P42 in the hippocampus, somatosensory cortex, and retrosplenial cortex shows that they were reduced in these brain regions at P14 but mostly less so at P42 in Fmr1 KO mice. However, some differential FMRP regulation of PNN development in these brain regions persists, perhaps caused by asynchrony in PNN development between brain regions in wild‐type animals. During development, aggrecan PNN levels in the brain were reduced in all brain regions in Fmr1 KO mice. Aggrecan mRNA levels were unchanged at these times, suggesting that FMRP is normally an activator of aggrecan mRNA translation. This hypothesis is buttressed by the observations that FMRP binds aggrecan mRNA and that ribosome profiling data show that aggrecan mRNA is associated with reduced numbers of ribosomes in Fmr1 KO mouse brain, indicating reduced translational efficiency. Moreover, aggrecan mRNA poly(A) tail length is also reduced in Fmr1 KO mouse brain, suggesting a relationship between polyadenylation and translational control. We propose a model where FMRP modulates PNN formation through translational up‐regulation of aggrecan mRNA polyadenylation and translation. [ABSTRACT FROM AUTHOR]

Environmental flows (e-flows) aim to mitigate the threat of altered hydrological regimes in river systems and connected waterbodies and are an important component of integrated strategies to address multiple threats to freshwater biodiversity. Expanding and accelerating implementation of e-flows can support river conservation and help to restore the biodiversity and resilience of hydrologically altered and water-stressed rivers and connected freshwater ecosystems. While there have been significant developments in e-flow science, assessment, and societal acceptance, implementation of e-flows within water resource management has been slower than required and geographically uneven. This review explores critical factors that enable successful e-flow implementation and biodiversity outcomes in particular, drawing on 13 case studies and the literature. It presents e-flow implementation as an adaptive management cycle enabled by 10 factors: legislation and governance, financial and human resourcing, stakeholder engagement and co-production of knowledge, collaborative monitoring of ecological and social-economic outcomes, capacity training and research, exploration of trade-offs among water users, removing or retrofitting water infrastructure to facilitate e-flows and connectivity, and adaptation to climate change. Recognising that there may be barriers and limitations to the full and effective enablement of each factor, the authors have identified corresponding options and generalizable recommendations for actions to overcome prominent constraints, drawing on the case studies and wider literature. The urgency of addressing flow-related freshwater biodiversity loss demands collaborative networks to train and empower a new generation of e-flow practitioners equipped with the latest tools and insights to lead adaptive environmental water management globally. Mainstreaming e-flows within conservation planning, integrated water resource management, river restoration strategies, and adaptations to climate change is imperative. The policy drivers and associated funding commitments of the Kunming–Montreal Global Biodiversity Framework offer crucial opportunities to achieve the human benefits contributed by e-flows as nature-based solutions, such as flood risk management, floodplain fisheries restoration, and increased river resilience to climate change. [ABSTRACT FROM AUTHOR]

Long-term environmental research networks are one approach to advancing local, regional, and global environmental science and education. A remarkable number and wide variety of environmental research networks operate around the world today. These are diverse in funding, infrastructure, motivating questions, scientific strengths, and the sciences that birthed and maintain the networks. Some networks have individual sites that were selected because they had produced invaluable long-term data, while other networks have new sites selected to span ecological gradients. However, all long-term environmental networks share two challenges. Networks must keep pace with scientific advances and interact with both the scientific community and society at large. If networks fall short of successfully addressing these challenges, they risk becoming irrelevant. The objective of this paper is to assert that the biogeosciences offer environmental research networks a number of opportunities to expand scientific impact and public engagement. We explore some of these opportunities with four networks: the International Long-Term Ecological Research Network programs (ILTERs), critical zone observatories (CZOs), Earth and ecological observatory networks (EONs), and the FLUXNET program of eddy flux sites. While these networks were founded and expanded by interdisciplinary scientists, the preponderance of expertise and funding has gravitated activities of ILTERs and EONs toward ecology and biology, CZOs toward the Earth sciences and geology, and FLUXNET toward ecophysiology and micrometeorology. Our point is not to homogenize networks, nor to diminish disciplinary science. Rather, we argue that by more fully incorporating the integration of biology and geology in long-term environmental research networks, scientists can better leverage network assets, keep pace with the ever-changing science of the environment, and engage with larger scientific and public audiences.

Eukaryotic translation is tightly regulated to ensure that protein production occurs at the right time and place. Recent studies on abnormal repeat proteins, especially in age-dependent neurodegenerative diseases caused by nucleotide repeat expansion, have highlighted or identified two forms of unconventional translation initiation: usage of AUG-like sites (near cognates) or repeat-associated non-AUG (RAN) translation. We discuss how repeat proteins may differ due to not just unconventional initiation, but also ribosomal frameshifting and/or imperfect repeat DNA replication, expansion, and repair, and we highlight how research on translation of repeats may uncover insights into the biology of translation and its contribution to disease.

The critical zone (CZ), the dynamic living skin of the Earth, extends from the top of the vegetative canopy through the soil and down to fresh bedrock and the bottom of the groundwater. All humans live in and depend on the CZ. This zone has three co-evolving surfaces: the top of the vegetative canopy, the ground surface, and a deep subsurface below which Earth's materials are unweathered. The network of nine CZ observatories supported by the US National Science Foundation has made advances in three broad areas of CZ research relating to the co-evolving surfaces. First, monitoring has revealed how natural and anthropogenic inputs at the vegetation canopy and ground surface cause subsurface responses in water, regolith structure, minerals, and biotic activity to considerable depths. This response, in turn, impacts aboveground biota and climate. Second, drilling and geophysical imaging now reveal how the deep subsurface of the CZ varies across landscapes, which in turn influences aboveground ecosystems. Third, several new mechanistic models now provide quantitative predictions of the spatial structure of the subsurface of the CZ.Many countries fund critical zone observatories (CZOs) to measure the fluxes of solutes, water, energy, gases, and sediments in the CZ and some relate these observations to the histories of those fluxes recorded in landforms, biota, soils, sediments, and rocks. Each US observatory has succeeded in (i) synthesizing research across disciplines into convergent approaches; (ii) providing long-term measurements to compare across sites; (iii) testing and developing models; (iv) collecting and measuring baseline data for comparison to catastrophic events; (v) stimulating new process-based hypotheses; (vi) catalyzing development of new techniques and instrumentation; (vii) informing the public about the CZ; (viii) mentoring students and teaching about emerging multidisciplinary CZ science; and (ix) discovering new insights about the CZ. Many of these activities can only be accomplished with observatories. Here we review the CZO enterprise in the United States and identify how such observatories could operate in the future as a network designed to generate critical scientific insights. Specifically, we recognize the need for the network to study network-level questions, expand the environments under investigation, accommodate both hypothesis testing and monitoring, and involve more stakeholders. We propose a driving question for future CZ science and a hubs-and-campaigns model to address that question and target the CZ as one unit. Only with such integrative efforts will we learn to steward the life-sustaining critical zone now and into the future.

Land use changes and reforestation impact soil phosphorus (P) distribution over extended periods. This study examines P distribution in forest development from 1957 to 2017 at the Calhoun Critical Zone Observatory in South Carolina. Tracking changes in 0–60 cm mineral soil P fractions through six samplings in eight continuously uncut plots and eight plots cut in 2007 (replanted in 2009), we hypothesized an increase in soil organic P (Po) over time in uncut plots, at the expense of HCl‐extractable P (i.e., Ca‐P). This hypothesis is based on the expectation of a transfer of P from easily accessible forms, possibly from historic fertilization to organic reservoirs within the soil. Meanwhile, we expect to lose Po in cut plots due to disturbance and residue decomposition, and increase bioavailable Mehlich‐III extractable P (Mehlich‐III P), resin inorganic phosphorus (Pi), and HCO3 Pi. Results revealed a continued decrease in Mehlich‐III P in uncut (38.7–15.7 µg g−1) and cut plots (25.2–17.7 µg g−1) within the 0–7.5 cm range. NaOH‐Po in uncut plots remained stable, while in cut plots, it increased by ∼4 µg g−1 in the 0–7.5 cm soil layer. Slowly cycling Po (NaOH‐Po) increased during forest regrowth in the cut plots, aligning with rising soil organic carbon and decreasing soil pH. Soil P changes indicate minor declines in available fractions during the initial three decades, with the HCl extractable (Ca‐P) fraction experiencing the highest depletion. In the later three decades, P associated with NaOH extraction (i.e., iron [Fe] and aluminum [Al] bound P) declined, although estimated mineral soil P removals during this period were less than accumulated P in tree biomass and forest floor. The total soil P decline from 1990 to 2017 was 34 kg ha−1, suggesting a growing role of P recycling from debris decomposition in supplying soil P to plants. Core Ideas: Phosphorus distribution in mineral soils is dynamic across a forest's developmental timeline from 1957 to 2017.Phosphorus recycling from forest floor and soil organic P contributes to plant nutrient supply.An agricultural fertilization legacy is still influencing long‐term phosphorus cycling.The decadal timescale of this study highlights the role of slowly cycling phosphorus fractions over extended time scales. [ABSTRACT FROM AUTHOR]

Gld2, a noncanonical cytoplasmic poly(A) polymerase, interacts with the RNA binding protein CPEB1 to mediate polyadenylation-induced translation in dendrites of cultured hippocampal neurons. Depletion of Gld2 from the hippocampus leads to a deficit in long-term potentiation evoked by theta burst stimulation. At least in mouse liver and human primary fibroblasts, Gld2 also 3' monoadenylates and thereby stabilizes specific miRNAs, which enhance mRNA translational silencing and eventual destruction. These results suggest that Gld2 would be likely to monoadenylate and stabilize miRNAs in the hippocampus, which would produce measurable changes in animal behavior. We now report that using Gld2 knockout mice, there are detectable alterations in specific miRNA monoadenylation in the hippocampus when compared to wild type, but that these modifications produce no detectable effect on miRNA stability. Moreover, we surprisingly find no overt change in animal behavior when comparing Gld2 knockout to wild-type mice. These data indicate that miRNA monoadenylation-mediated stability is cell type-specific and that monoadenylation has no measurable effect on higher cognitive function.