216 results on '"Ricci MA"'
Search Results
2. Multi-analytical non-destructive investigation of pictorial apparatuses of 'Villa della Piscina' in Rome
- Author
-
Sbroscia, M, Cestelli-Guidi, M, Colao, F, Falzone, S, Gioia, C, Gioia, P, Marconi, C, Mirabile, D, Gattia, Loreti, Em, Marinelli, M, Missori, M, Persia, F, Pronti, L, Romani, M, Sodo, A, Verona-Rinati, G, Ricci, Ma, Fantoni, R, Sbroscia, M., Cestelli-Guidi, M., Colao, F., Falzone, S., Gioia, C., Gioia, P., Marconi, C., Mirabile Gattia, D., Loreti, E. M., Marinelli, M., Missori, M., Persia, F., Pronti, L., Romani, M., Sodo, A., Verona-Rinati, G., Ricci, M. A., and Fantoni, R.
- Subjects
Pigments ,Painting ,media_common.quotation_subject ,Settore FIS/07 ,Material provenance ,Multi-analytical approach ,Art ,Archaeology ,Analytical Chemistry ,Style (visual arts) ,Conservation ,Palette (painting) ,Pigment ,Non-invasive analytical techniques ,Non-invasive analytical technique ,Non destructive ,Roman wall painting ,Roman wall paintings ,Spectroscopy ,media_common - Abstract
Here we present a multi-analytical approach, that makes use of spectroscopic and imaging techniques, aiming at characterising the wall paintings of the “Villa della Piscina”, a Roman archaeological site in Rome. More specifically, we focus the attention on plaster fragments dated in the narrow temporal window bounded between the second half of I century and the first half of II century A.D., according to stylistic and archaeological standpoint. This investigation aims at supporting archaeologists in the reconstruction of the decorative motif of the Roman Villa, by studying the composition, the provenance and the style of the decorative phases of the analysed fragments. The presence of conservation and restoration treatments, as protective materials, is also investigated. Our study evidences a rich color palette and a refined use of mixtures of minerals and pigments.
- Published
- 2020
3. Corrigendum: The new Italian registry of infantile thrombosis (RITI): a reflection on its journey, challenges and pitfalls
- Author
-
Maria Federica Pelizza, Matteo Martinato, Anna Rosati, Margherita Nosadini, Paola Saracco, Paola Giordano, Matteo Luciani, Laura Ilardi, Donatella Lasagni, Angelo Claudio Molinari, Rossana Bagna, Antonella Palmieri, Luca Antonio Ramenghi, Massimo Grassi, Mariella Magarotto, Federica Magnetti, Andrea Francavilla, Giuseppe Indolfi, Agnese Suppiej, Chiara Gentilomo, Roberta Restelli, Antonella Tufano, Daniela Tormene, Jacopo Norberto Pin, Clarissa Tona, Davide Meneghesso, Lidia Rota, Marta Conti, Giovanna Russo, Giulia Lorenzoni, Dario Gregori, Stefano Sartori, Paolo Simioni, Collaborators of the R.I.T.I. (Italian and Registry of Infantile Thrombosis), Accorsi Patrizia, Aceto Gabriella, Agnoletti Gabriella, Agostini Manuela, Alfarano Angela, Altieri Elena, Amador Carolina, Antonelli Camilla, Arena Vittoria, Asta Francesca, Baggio Laura, Ballardini Elisa, Baracetti Margherita, Baraldi Eugenio, Barberis Laura, Barisone Elena, Basso Anne Letizia, Battajon Nadia, Bersani Iliana, Biddeci Giada, Biffanti Roberta, Bonardi Claudia Maria, Bonaudo Roberto, Boniver Clementina, Boscarol Gianluca, Bottino Roberto, Bravar Giulia, Brizzi Ilaria, Brolatti Noemi, Braguglia Annabella, Guaragni Brunetta, Bugin Samuela, Calvo Pier Luigi, Capasso Antonella, Capodiferro Donatella, Cappelleri Alessia, Cascarano Maria Teresa, Casellato Susanna, Casini Tommaso, Catarzi Serena, Cavaliere Elena, Cavicchiolo Maria Elena, Celestino Silvia, Celle Maria Elena, Centonze Nicola, Cerutti Alessia, Chakrokh Roksana, Offer Chiara, Chiodin Elisabetta, Chirico Gaetano, Chukhlantseva Natalia, Cifarelli Paola, Cinelli Giulia, Coinu Marisa, Colonna Clara, Comito Donatella, Corato Alessandra, Cordelli Duccio Maria, Crichiutti Giovanni, Cursio Ida, Dagri Arianna, De Maria Beatrice, Del Borrello Giovanni, Di Rienzo Francesca, Doglioni Nicoletta, Dolcemascolo Valentina, Dotta Andrea, Drigo Paola, Drimaco Pietro, Ellero Serena, Falcone Alessandra, Fantauzzi Ambra, Farinasso Daniela, Ferilli Michela, Festa Silvia, Fischer Maximilian, Foiadelli Thomas, Fotzi Ilaria, Francavilla Rosa, Freschi Paola, Gaffuri Marcella, Gallo Elena, Gamalero Lisa, Gandioli Claudia, Garuccio Sergio, Gentile Diletta, Ghionzoli Marco, Giliberti Paola, Greco Filippo, Guariento Chiara, Guidotti Isotta, Iodice Alessandro, Janes Augusta, Laghi Elena, Lampugnani Elisabetta, Lassandro Giuseppe, Laverda Anna Maria, Lazzerotti Alessandra, Lo Tartaro Meragliotta Patrizia, Lombardini Martina, Lorenzon Eleonora, Mainini Nicoletta, Massoud Michela, Materia Valeria, Mattera Raffaele, Mauro Isabella, Melani Federico, Meli Mariaclaudia, Messina Giovanni, Monticone Sonia, Moras Marzia, Negro Ilaria, Olzai Giorgio, Pancani Simone, Pandolfi Maria, Passariello Annalisa, Passarini Alice, Passone Eva, Pastorino Myriam, Pegoraro Veronica, Pennoni Serena, Perilongo Giorgio, Pozzessere Anna, Pruna Dario, Pusiol Anna, Putti Maria Caterina, Rabbone Ivana, Radicioni Maurizio, Renna Salvatore, Ricci Maria Luisa, Rimini Alessandro, Rivellini Sara, Rustioni Gianluca, Salvadori Sabrina, Santoiemma Valentina, Santoro Nicola, Schiavulli Michele, Sebellin Sofia, Sesta Michela, Soffiati Massimo, Sorbo Monica, Spanedda Giuseppina, Stangalini Valeria, Stasolla Salvatore, Tanzi Giorgia, Testa Tiziana, Teutonico Federica, Timpani Giuseppina, Toldo Irene, Trapani Sandra, Vaccari Roberto, Vecchi Marilena, Vento Giovanni, Veraldi Daniele, Villa Giovanna, Visintin Gianluca, Zambelloni Cesare, and Zellini Francesco
- Subjects
thrombosis ,stroke ,children ,pediatric ,registry ,thromboembolism ,Pediatrics ,RJ1-570 - Published
- 2024
- Full Text
- View/download PDF
4. The new Italian registry of infantile thrombosis (RITI): A reflection on its journey, challenges and pitfalls
- Author
-
Maria Federica Pelizza, Matteo Martinato, Anna Rosati, Margherita Nosadini, Paola Saracco, Paola Giordano, Matteo Luciani, Laura Ilardi, Donatella Lasagni, Angelo Claudio Molinari, Rossana Bagna, Antonella Palmieri, Luca Antonio Ramenghi, Massimo Grassi, Mariella Magarotto, Federica Magnetti, Andrea Francavilla, Giuseppe Indolfi, Agnese Suppiej, Chiara Gentilomo, Roberta Restelli, Antonella Tufano, Daniela Tormene, Jacopo Norberto Pin, Clarissa Tona, Davide Meneghesso, Lidia Rota, Marta Conti, Giovanna Russo, Giulia Lorenzoni, Dario Gregori, Stefano Sartori, Paolo Simioni, Collaborators of the R.I.T.I. (Italian Registry of Infantile Thrombosis), Accorsi Patrizia, Aceto Gabriella, Agnoletti Gabriella, Agostini Manuela, Alfarano Angela, Altieri Elena, Amador Carolina, Antonelli Camilla, Arena Vittoria, Asta Francesca, Baggio Laura, Ballardini Elisa, Baracetti Margherita, Baraldi Eugenio, Barberis Laura, Barisone Elena, Basso Anne Letizia, Battajon Nadia, Bersani Iliana, Biddeci Giada, Biffanti Roberta, Bonardi Claudia Maria, Bonaudo Roberto, Boniver Clementina, Boscarol Gianluca, Bottino Roberto, Bravar Giulia, Brizzi Ilaria, Brolatti Noemi, Braguglia Annabella, Guaragni Brunetta, Bugin Samuela, Calvo Pier Luigi, Capasso Antonella, Capodiferro Donatella, Cappelleri Alessia, Cascarano Maria Teresa, Casellato Susanna, Casini Tommaso, Catarzi Serena, Cavaliere Elena, Cavicchiolo Maria Elena, Celestino Silvia, Celle Maria Elena, Centonze Nicola, Cerutti Alessia, Chakrokh Roksana, Offer Chiara, Chiodin Elisabetta, Chirico Gaetano, Chukhlantseva Natalia, Cifarelli Paola, Cinelli Giulia, Coinu Marisa, Colonna Clara, Comito Donatella, Corato Alessandra, Cordelli Duccio Maria, Crichiutti Giovanni, Cursio Ida, Dagri Arianna, De Maria Beatrice, Del Borrello Giovanni, Di Rienzo Francesca, Doglioni Nicoletta, Dolcemascolo Valentina, Dotta Andrea, Drigo Paola, Drimaco Pietro, Ellero Serena, Falcone Alessandra, Fantauzzi Ambra, Farinasso Daniela, Ferilli Michela, Festa Silvia, Fischer Maximilian, Foiadelli Thomas, Fotzi Ilaria, Francavilla Rosa, Freschi Paola, Gaffuri Marcella, Gallo Elena, Gamalero Lisa, Gandioli Claudia, Garuccio Sergio, Gentile Diletta, Ghionzoli Marco, Giliberti Paola, Greco Filippo, Guariento Chiara, Guidotti Isotta, Iodice Alessandro, Janes Augusta, Laghi Elena, Lampugnani Elisabetta, Lassandro Giuseppe, Laverda Anna Maria, Lazzerotti Alessandra, Lo Tartaro Meragliotta Patrizia, Lombardini Martina, Lorenzon Eleonora, Mainini Nicoletta, Massoud Michela, Materia Valeria, Mattera Raffaele, Mauro Isabella, Melani Federico, Meli Mariaclaudia, Messina Giovanni, Monticone Sonia, Moras Marzia, Negro Ilaria, Olzai Giorgio, Pancani Simone, Pandolfi Maria, Passariello Annalisa, Passarini Alice, Passone Eva, Pastorino Myriam, Pegoraro Veronica, Pennoni Serena, Perilongo Giorgio, Pozzessere Anna, Pruna Dario, Pusiol Anna, Putti Maria Caterina, Rabbone Ivana, Radicioni Maurizio, Renna Salvatore, Ricci Maria Luisa, Rimini Alessandro, Rivellini Sara, Rustioni Gianluca, Salvadori Sabrina, Santoiemma Valentina, Santoro Nicola, Schiavulli Michele, Sebellin Sofia, Sesta Michela, Soffiati Massimo, Sorbo Monica, Spanedda Giuseppina, Stangalini Valeria, Stasolla Salvatore, Tanzi Giorgia, Testa Tiziana, Teutonico Federica, Timpani Giuseppina, Toldo Irene, Trapani Sandra, Vaccari Roberto, Vecchi Marilena, Vento Giovanni, Veraldi Daniele, Villa Giovanna, Visintin Gianluca, Zambelloni Cesare, and Zellini Francesco
- Subjects
thrombosis ,stroke ,children ,pediatric ,registry ,thromboembolism ,Pediatrics ,RJ1-570 - Abstract
IntroductionThrombotic events in neonates and children represent a rare although severe occurrence in view of the associated risk of mortality and sequelae. Quality evidence is limited in this field, and registry studies provide an essential base for research. The aim of this paper is to present the new Italian Registry of Infantile Thrombosis (RITI), set it into the scene of international thrombosis and stroke registries, and provide some insight on the challenges associated with registry management.MethodsWe present the detailed structure and content of the new RITI registry, a brief overview of its main data, and a reflection on its features, pitfalls and the main challenges related to its management.ResultsThe RITI, initially started in 2007 and officially re-launched in 2017 after structural modifications, is a non-interventional retrospective and prospective registry study collecting data on neonatal and pediatric patients (0–18 years) who experienced a systemic or cerebral thrombotic event in Italy. The RITI is managed by a multidisciplinary team with expertise in pediatric thrombosis, and participation is open to all Italian physicians, on a voluntary basis. The overall aim of the registry is to acquire new evidence to better characterize the population of children with thrombotic events and improve their management and outcome. 48 Italian pediatric and intensive care units are actively involved in the RITI, including 85 medical doctors from 16 Italian regions. A total of 1,001 neonates and children affected by cerebral or systemic thrombosis have been enrolled.DiscussionThe RITI is one of the largest available European registries of neonatal and pediatric thrombosis. National registries like the RITI represent a model for the study of rare conditions based on multidisciplinary and multicenter collaboration, aimed at overcoming the limitations due to small populations of patients, and creating a network of experts for patient referral and continuous education. Moreover, registry studies have a pivotal role in the research on pediatric thrombosis, due to the limited feasibility of high-quality studies. In our experience, the main critical stages, pitfalls and challenges in registry management include adequate registry designing, diffusion, data completeness and quality control.
- Published
- 2023
- Full Text
- View/download PDF
5. A yeast synthetic network for in-vivo assessment of reverse engineering and modelling
- Author
-
CANTONE, IRENE, Marucci L., Iorio F., Ricci MA, Belcastro V, Cosma M.P., DI BERNARDO, MARIO, SANTINI, STEFANIA, DI BERNARDO, DIEGO, Cantone, Irene, Marucci, L., Iorio, F., Ricci, Ma, Belcastro, V, DI BERNARDO, Mario, Santini, Stefania, DI BERNARDO, Diego, and Cosma, M. P.
- Subjects
identificazione ,reti genetiche ,biologia sintentica - Abstract
Systems biology approaches are extensively used to model and reverse engineer gene regulatory networks from experimental data. Conversely, synthetic biology allows ‘‘de novo’’ construction of a regulatory network to seed new functions in the cell. At present, the usefulness and predictive ability of modeling and reverse engineering cannot be assessed and compared rigorously. We built in the yeast Saccharomyces cerevisiae a synthetic network, IRMA, for in vivo ‘‘benchmarking’’ of reverse-engineering and modeling approaches. The network is composed of five genes regulating each other through a variety of regulatory interactions; it is negligibly affected by endogenous genes, and it is responsive to small molecules. We measured time series and steady-state expression data after multiple perturbations. These data were used to assess state-of-the-art modeling and reverse-engi- neering techniques. A semiquantitative model was able to capture and predict the behavior of the network. Reverse engineering based on differential equations and Bayesian networks correctly inferred regulatory interactions from the experimental data.
- Published
- 2009
6. CO2–water supercritical mixtures: Test of a potential model against neutron diffraction data
- Author
-
LO CELSO, Fabrizio, TRIOLO, Roberto, FERRANTE, Francesco, BOTTI, A, BRUNI, F, MANCINELLI, R, RICCI, MA, SOPER, AK, LO CELSO, F, TRIOLO, R, FERRANTE, F, BOTTI, A, BRUNI, F, MANCINELLI, R, RICCI, MA, and SOPER, AK
- Published
- 2007
7. Study of alpha-lipoic acid penetration in the human aqueous after topical administration
- Author
-
Cagini, Carlo, Leontiadis, A, Ricci, Ma, Bartolini, A, Dragoni, A, and Pellegrino, Roberto Maria
- Subjects
Aged, 80 and over ,Male ,Thioctic Acid ,Administration, Topical ,Middle Aged ,Antioxidants ,Gas Chromatography-Mass Spectrometry ,Aqueous Humor ,Cornea ,Calibration ,Humans ,Female ,Ophthalmic Solutions ,Aged - Abstract
To determine the concentration of alpha-lipoic acid in the aqueous and investigate if its topical instillation can increase quantities.A total of 70 patients selected to undergo cataract surgery were randomly divided into two groups. Group 1 was used as a control group; for the patients in Group 2, a single instillation of alpha-lipoic acid eye drops (1%) was administered. Immediately before surgery, an aliquot of 40-120 microL of aqueous was aspirated. The individual aspirations were combined to constitute pools representing time intervals with respect to administration. The levels of alpha-lipoic acid in the aqueous were measured using gas chromatography/mass spectrometry.Pool 0 was created by combining the samples of aqueous obtained from the patients in Group 1, the control group; and the level of alpha-lipoic acid was 27.5 + 2.6 ng/mL. In the other pools, the time interval between the administration of the eye drops and sampling was, respectively, 23 min, 53 min, 72 min, 93 min and 114 min; and the level of alpha-lipoic acid was 33.0 + 10.8 ng/mL, 52.0 + 2.5 ng/mL, 86.7 + 2.5 ng/mL, 91.2 + 2.5 ng/mL and 80.3 + 2.5 ng/mL.Our study demonstrates the presence of alpha-lipoic acid in the aqueous and indicates that its concentration increases after it is administered in the form of eye drops, reaching maximum values after around 93 min. The concentrations that are achieved in the anterior chamber allow us to theorize the possibility of exploiting the antioxidant properties of alpha-lipoic acid.
- Published
- 2010
8. Neutron diffraction study of aqueous Laponite suspensions at the NIMROD diffractometer
- Author
-
Tudisca, V, Bruni, F, Scoppola, E, Angelini, R, Ruzicka, B, Zulian, L, Soper, A, Ricci, M, Soper, AK, Ricci, MA, ZULIAN, LAURA, Tudisca, V, Bruni, F, Scoppola, E, Angelini, R, Ruzicka, B, Zulian, L, Soper, A, Ricci, M, Soper, AK, Ricci, MA, and ZULIAN, LAURA
- Abstract
The process of dynamical arrest, leading to formation of different arrested states such as glasses and gels, along with the closely related process of aging, is central for both basic research and technology. Here we report on a study of the time-dependent structural evolution of two aqueous Laponite clay suspensions at different weight concentrations. Neutron diffraction experiments have been performed with the near and intermediate range order diffractometer (NIMROD) that allows studies of the structure of liquids and disordered materials over a continuous length scale ranging from 1 to 300 A° , i.e., from the atomistic to the mesoscopic scales. NIMROD is presently a unique diffractometer, bridging the length scales traditionally investigated by small angle neutron scattering or small angle x-ray scattering with that accessible by traditional diffractometers for liquids. Interestingly, we have unveiled a signature of aging of both suspensions in the length scale region of NIMROD. This phenomenon, ascribed to sporadic contacts between Laponite platelets at long times, has been observed with the sample arrested as gel or as repulsive glass. Moreover, water molecules within the layers closest to Laponite platelets surface show orientational and translational order, which maps into the crystalline structure of Laponite.
- Published
- 2014
9. Implications of Mini-EUSO measurements for a space-based observation of UHECRs
- Author
-
Bertaina Mario Edoardo, Barghini Dario, Battisti Matteo, Belov Alexander, Bianciotto Marta, Bisconti Francesca, Blaksley Carl, Blin Sylvie, Bolmgren Karl, Cambiè Giorgio, Capel Francesca, Casolino Marco, Churilo Igor, Crisconio Marino, De La Taille Christophe, Ebisuzaki Toshikazu, Eser Johannes, Fenu Francesco, Filippatos George, Franceschi Massimo Alberto, Fuglesang Christer, Golzio Alessio, Gorodetzky Philippe, Kajino Fumiyoshi, Kasuga Hiroshi, Klimov Pavel, Kungel Viktoria, Kuznetsov Vladimir, Manfrin Massimiliano, Marcelli Laura, Mascetti Gabriele, Marszał Włodzimierz, Mignone Marco, Miyamoto Hiroko, Murashov Alexey, Napolitano Tommaso, Ohmori Hitoshi, Olinto Angela, Parizot Etienne, Picozza Piergiorgio, Piotrowski Lech Wiktor, Plebaniak Zbigniew, Prévôt Guillaume, Reali Enzo, Ricci Marco, Romoli Giulia, Sakaki Naoto, Sharakin Sergei, Shinozaki Kenji, Szabelski Jacek, Takizawa Yoshiyuki, Valentini Giovanni, Vrabel Michal, Wiencke Lawrence, and Zotov Mikhail
- Subjects
Physics ,QC1-999 - Abstract
Mini-EUSO is the first mission of the JEM-EUSO program on board the International Space Station. It was launched in 2019 and it is currently located in the Russian section (Zvezda module) of the station and viewing our planet from a nadir-facing UV-transparent window. The instrument is based on the concept of the original JEM-EUSO mission and consists of an optical system employing two Fresnel lenses and a focal surface composed of 36 Multi-Anode Photomultiplier tubes, 64 channels each, for a total of 2304 channels with single photon counting sensitivity and an overall field of view of 44° × 44°. Mini-EUSO can map the night-time Earth in the near UV range (predominantly between 290 nm and 430 nm), with a spatial resolution of about 6.3 km and different temporal resolutions of 2.5 µ, 320 µs and 41 ms. Mini-EUSO observations are extremely important to better assess the potential of a space-based detector in studying Ultra-High Energy Cosmic Rays (UHECRs) such as K-EUSO and POEMMA. In this contribution we focus the attention on UV measurements, the observation of clouds and of certain categories of events that Mini-EUSO triggers with the shortest temporal resolution. We place them in the context of UHECR observations from space, namely the estimation of exposure and sensitivity to Extensive Air Showers.
- Published
- 2023
- Full Text
- View/download PDF
10. The JEM-EUSO Program for UHECR Studies from Space
- Author
-
Parizot Etienne, Casolino Marco, Picozza Piergiorgio, Ebisuzaki Toshikazu, Bertaina Mario Edoardo, Fuglesang Christer, Haungs Andreas, Kajino Fumiyoshi, Klimov Pavel, Olinto Angela, Ricci Marco, Sagawa Hiroyuki, Szabelski Jacek, and Wiencke Lawrence
- Subjects
Physics ,QC1-999 - Abstract
To take up the challenge of understanding the origin of the ultra-high-energy cosmic rays (UHECRs), new observational means appear necessary. The JEM-EUSO Collaboration has undertaken to open the space road to UHECR studies. For more than a decade, it has been developing a realistic program to measure the UHECRs from space with unprecedented aperture, together with complementary scientific objectives in a broader multidisciplinary context. Several intermediate missions have already been completed (on the ground: EUSO-TA; under stratospheric ballons: EUSO-Balloon and EUSO-SPB1; in space: TUS, and on-board the ISS: MINI-EUSO), and others are in preparation for flight (EUSO-SPB2), under review (K-EUSO: currently on hold), or proposed for the next decade (POEMMA). We report on the general status of the JEM-EUSO program, underlining that its technology has now reached operational maturity, and is ready for actual cosmic-ray shower detection from above.
- Published
- 2023
- Full Text
- View/download PDF
11. An end-to-end in-flight calibration of Mini-EUSO detector
- Author
-
Miyamoto Hiroko, Battisti Matteo, Barghini Dario, Belov Alexander, Bertaina Mario, Bianciotto Marta, Bisconti Francesca, Blaksley Carl, Blin Sylvie, Bolmgren Karl, Cambiè Giorgio, Capel Francesca, Casolino Marco, Churilo Igor, Crisconio Marino, De La Taille Christophe, Ebisuzaki Toshikazu, Eser Johannes, Fenu Francesco, Filippatos George, Franceschi Massimo Alberto, Fuglesang Christer, Golzio Alessio, Gorodetzky Philippe, Kajino Fumiyoshi, Kasuga Hiroshi, Klimov Pavel, Kungel Viktoria, Kuznetsov Vladimir, Manfrin Massimiliano, Marcelli Laura, Mascetti Gabriele, Marszał Włodzimierz, Mignone Marco, Murashov Alexey, Napolitano Tommaso, Ohmori Hitoshi, Olinto Angela, Parizot Etienne, Picozza Piergiorgio, Piotrowski Lech Wiktor, Plebaniak Zbigniew, Prévôt Guillaume, Reali Enzo, Ricci Marco, Romoli Giulia, Sakaki Naoto, Sharakin Sergei, Shinozaki Kenji, Szabelski Jacek, Takizawa Yoshiyuki, Valentini Giovanni, Vrabel Michal, Wincke Lawrence, and Zotov Mikhail
- Subjects
Physics ,QC1-999 - Abstract
Mini-EUSO is a wide Field-of-View (FoV, 44°) telescope currently in operation from a nadir-facing UV-transparent window in the Russian Zvezda module on the International Space Station (ISS). It is the first detector of the JEM-EUSO program deployed on the ISS, launched in August 2019. The main goal of Mini-EUSO is to measure the UV emissions from the ground and atmosphere, using an orbital platform. Mini-EUSO is mainly sensitive in the 290–430 nm bandwidth. Light is focused by a system of two Fresnel lenses of 25 cm diameter each on the Photo-Detector-Module (PDM), which consists of an array of 36 Multi-Anode Photomultiplier Tubes (MAPMTs), arranged in blocks of 2 × 2 called Elementary Cells (ECs), for a total of 2304 pixels working in photon counting mode, in three different time resolutions of 2.5 µs (defined as 1 Gate Time Unit, GTU), 320 µs and 40.96 ms operating in parallel. In the longest time scale, the data is continuously acquired to monitor the UV emission of the Earth. It is best suited for the observation of ground sources and therefore has been used for the observational campaigns of the ground-based UV flasher in order to perform an end-to-end calibration of Mini-EUSO. In this contribution, the assembled UV flasher, the operation of the field campaign and the analysis of the obtained data are presented. The result is compared with the overall effi ciency computed from the expectations which takes into account the atmospheric attenuation and the parametrisation of different effects such as the optics effi ciency, the MAPMT detection effi ciency, BG3 filter transmittance and the transparency of the ISS window.
- Published
- 2023
- Full Text
- View/download PDF
12. Quantum effects in the structural properties of supercritical He4
- Author
-
Nardone, M, Bellissent Funel, Mc, Filabozzi, A, Ricci, Fp, and Ricci, Ma
- Subjects
NEUTRON-DIFFRACTION ,LIQUID-HE-4 ,SCATTERING ,DEPENDENCE ,DENSITY ,SYSTEMS ,HELIUM ,Settore FIS/01 - Fisica Sperimentale - Published
- 1994
13. Quantum and classical behavior of single-particle dynamics in dense liquid He4
- Author
-
Andreani, C, Filabozzi, A, Nardone, M, Ricci, Ma, and Mayers, J
- Subjects
Settore FIS/01 - Fisica Sperimentale - Published
- 1994
14. Management of Dietary Habits and Diarrhea in Fap Individuals: A Mediterranean Low-Inflammatory Dietary Intervention
- Author
-
Ciniselli Chiara Maura, Bruno Eleonora, Oliverio Andreina, Baldassari Ivan, Pastori Marta, Signoroni Stefano, Vitellaro Marco, Ricci Maria Teresa, Milione Massimo, Cattaneo Laura, Gariboldi Manuela, Mancini Andrea, Rivoltini Licia, Morelli Daniele, Pasanisi Patrizia, and Verderio Paolo
- Subjects
FAP individuals ,Mediterranean low-inflammatory diet ,dietary questionnaires ,dietary adherence ,diarrheal discharges ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Background: A total colectomy and a frequent life-long endoscopic surveillance are guaranteed to patients with Familial Adenomatous Polyposis (FAP) to reduce their risk of duodenal and rectal stump cancers. However, after surgery, individuals with FAP suffer from an increased number of diarrheal discharges that force them to dietary restrictions. A non-randomized pilot study was conducted to assess whether a three-month low-inflammatory Mediterranean dietary intervention reduces gastro-intestinal markers of inflammation in FAP individuals. The aim of the present work is to evaluate the participant’s adherence to the proposed dietary recommendations and the change in their number of diarrheal discharges. Methods: 26 FAP individuals aged >18 years, who underwent a total colectomy with ileo-rectal anastomosis and were involved in the surveillance program at the Fondazione IRCCS Istituto Nazionale Tumori of Milan, were included in the present analysis. Results: FAP individuals significantly reduced the Not recommended foods (p-value: 0.002) and increased the consumption of the Recommended ones (p-value: 0.075). The adherence to the proposed dietary recommendations was accompanied by a significant decrease in the number of diarrheal discharges (p-value: 0.008). Conclusions: This study suggests that adhering to a low-inflammatory Mediterranean diet has a potential protective effect on the number of diarrheal discharges in FAP individuals.
- Published
- 2021
- Full Text
- View/download PDF
15. Applied research: effectiveness of lectures attended via interactive video conferencing versus in-person in preparing third-year internal medicine clerkship students for clinical practice examinations (cpx)
- Author
-
Bertsch TF, Callas PW, Rubin A, Caputo MP, and Ricci MA
- Abstract
Background: The current practice in medical education is to place students at off-site locations. The effectiveness of these students attending remote lectures using interactive videoconferencing needs to be evaluated. Purpose: To determine whether lecture content covering clinical objectives is learned by medical students located at remote sites. Methods: During the University of Vermont medicine clerkship, 52 medical students attended lectures both in person and via 2-way videoconferencing over a telemedicine network. The study used a crossover design, such that all students attended half of the lectures in person and half using videoconferencing. At the end of the clerkship, students were assessed via a Clinical Practice Examination (CPX), with each student completing 1 exam for material learned in person and 1 for material learned over telemedicine. Results: Exam scores did not differ for the 2 lecture modes, with a mean score of 76% for lectures attended in person and a mean score of 78% for lectures attended via telemedicine (p = 0.66). Conclusions: Students learn content focused on clinical learning objectives as well using videoconferencing as they do in the traditional classroom setting. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
16. Medical student evaluations of lectures attended in person or from rural sites via interactive videoconferencing.
- Author
-
Callas PW, Bertsch TF, Caputo MP, Flynn BS, Doheny-Farina S, and Ricci MA
- Abstract
BACKGROUND: Interactive videoconferencing may be an effective way for medical students on remote rotations to attend teaching sessions at the main campus. PURPOSE: To compare medical student evaluations of lectures for those attending in person and those attending through interactive videoconferencing. METHODS: Lecture evaluations were completed by medical students on University of Vermont College of Medicine clinical clerkship rotations. Students on clerkships at rural sites attended lectures using our telemedicine network. Responses from in-person and remote attendees were compared. RESULTS: Evaluation forms for 110 lectures were received from 648 in-person and 255 remote attendees. All evaluation items were rated 'good' or 'excellent' by at least 95% of in-person attendees. Over 90% of remote attendees rated nontelemedicine evaluation items, such as appropriateness of lecture topic for students, as good or excellent. Ratings of telemedicine-specific questions, such as ability to hear the lecturer, were lower. CONCLUSIONS: Level of satisfaction was high for most aspects of remote lecture attendance, although not quite as high as for in-person attendance. Improved technical reliability would likely increase remote attendee satisfaction. Overall, lecture attendance using videoconferencing was found to be an acceptable alternative to travel for medical students in rural clerkships. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
17. Effects of home versus supervised exercise for patients with intermittent claudication.
- Author
-
Savage P, Ricci MA, Lynn M, Gardner A, Knight S, Brochu M, and Ades P
- Published
- 2001
18. The exploitation of CFD legacy for the meridional analysis and design of modern gas and steam turbines
- Author
-
Ricci Martina, Pacciani Roberto, and Marconcini Michele
- Subjects
Environmental sciences ,GE1-350 - Abstract
In the last decades, the consolidation of 3D CFD approaches in the industrial design practices has progressively moved throughflow codes from the top of design systems to somewhere in between first development stages and the final aerodynamic optimizations. Despite this trend and the typical limitations of traditional throughflow methods, designers tend to still consider such methods as fundamental tools for drafting a credible aero-design in a short turnaround time. Recently a considerable attention has been devoted to CFDbased throughflow codes as suitable means to widen the range of applicability of these tools while smoothing the predictive gap with successive threedimensional flow analyses. The present paper retraces the development and some applications of a modern and complete CFD-based throughflow solver specifically tuned for multistage axial turbine design. The code solves the axisymmetric Euler equations with an original treatment of tangential blockage and body force. It inherits its numerical scheme from a state-of-the-art CFD solver (TRAF code) and incorporates real gas capabilities, three-dimensional flow features (e.g. secondary flows, tip leakage effects), coolant flow injections, and radial mixing models. Also geometric features of actual blades, like fillets, part-span shrouds, and snubbers, are accounted for by suitable models. The capabilities of the code are demonstrated by discussing a significant range of test cases and industrial applications. They include single stage configurations and entire multistage modules of steam turbines, with flow conditions ranging from subsonic to supersonic. Computational strategies for design and off-design analyses will be presented and discussed. The reliability and accuracy of the method is assessed by comparing throughflow results with 3D CFD calculations and experimental data. A good agreement in terms of overall performance and spanwise distributions is achieved in both design and off-design operating conditions.
- Published
- 2020
- Full Text
- View/download PDF
19. Prophylactic vena cava filter insertion in selected high-risk orthopaedic trauma patients.
- Author
-
Rogers FB, Shackford SR, Ricci MA, Huber BM, Atkins T, Rogers, F B, Shackford, S R, Ricci, M A, Huber, B M, and Atkins, T
- Published
- 1977
20. Use of the rectus abdominis muscle flap to fill a retroperitoneal defect following blast injury.
- Author
-
Talarczyk MR, Ricci MA, Talarczyk, Matthew R, and Ricci, Michael A
- Abstract
Wartime injuries from explosive devices have created the need for atypical responses to devastating and unusual injuries. We report a case of an explosive abdominal injury that produced a huge defect in the posterior abdominal wall which was ultimately repaired with a rectus abdominus flap, an usual use of this versatile muscle flap. The rectus abdominus muscle may be another tool available for the repair of wartime injuries. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
21. Raman spectrum of water in the translational and librational region: II- Temperature evolution of the isotropic component
- Author
-
Benassi, P, Mazzacurati, V, Nardone, M, Ricci, Ma, Ruocco, G, DE SANTIS, A, Frattini, Romana, and Sampoli, M.
- Published
- 1987
22. COLLECTIVE DYNAMICS IN LIQUID WATER
- Author
-
Ricci, Ma, Giancarlo Ruocco, Rocca, D., Vallauri, R., Ricci, Maria Antonietta, Rocca, D., Ruocco, G., and Vallauri, R.
23. Influência da preservação de pele em cirurgia conservadora por câncer de mama sobre as taxas de recorrência local e a distância
- Author
-
Ricci Marcos Desidério, Pinotti José Aristodemo, Carvalho Filomena Marino, Pinotti Marianne, and Giribela Arícia Helena G.
- Subjects
Mama/câncer ,Cirurgia conservadora ,Câncer/prognóstico ,Gynecology and obstetrics ,RG1-991 - Abstract
OBJETIVO: avaliar a taxa de recorrência local, regional e a distância de nova técnica cirúrgica para tratamento conservador do câncer de mama de estádio inicial. A técnica é baseada na ressecção segmentar com dissecção axilar e preservação de pele por incisão periareolar única. MÉTODOS: cento e dezenove pacientes com câncer de mama de estádio I e II foram incluídas neste estudo. O grupo estudo foi constituído por 57 pacientes que se submeteram à cirurgia pela técnica proposta e 62 pacientes submetidas a quadrantectomia clássica constituíram o grupo controle. A radioterapia pós-operatória e o boost foram realizados em ambos os grupos. A média de seguimento foi de 50,1 meses para o grupo estudo e 51,2 para o grupo controle. As taxas de recidiva, sobrevida global e sobrevida livre de doença foram analisadas e comparadas entre os dois grupos. RESULTADOS: a taxa de recidiva local no período foi de 3,5% para o grupo estudo e 4,8% para o grupo controle. Não houve diferença significativa entre os grupos nas taxas de sobrevida livre de doença e sobrevida global. CONCLUSÕES: a técnica de ressecção segmentar com dissecção axilar por incisão única periareolar, preservando pele, não mostrou diferença, em relação à quadrantectomia clássica, nas taxas de recidiva, sobrevida global e sobrevida livre de doença.
- Published
- 2003
24. Different ways to repair the mitral valve with artificial chordae: a systematic review
- Author
-
Rose David, Ricci Massimo, Tudisco Antonella, Bizzarri Federico, and Frati Giacomo
- Subjects
Surgery ,RD1-811 ,Anesthesiology ,RD78.3-87.3 - Abstract
Abstract Myxomatous mitral regurgitation (type II Carpentier's functional classification) affects about 1-2% of the population. This represents a very common indication for valve surgery resulting in a low percentage of repairs compared to replacement which is actually performed. In the last decades, several methods for mitral valve repair have been developed, to make the surgical feasibility easier, improve the long-term follow-up thus avoiding the need for reoperations. A very interesting method is represented by the combination of various valve repair techniques, depending on the involvement of the anterior, posterior, or both leaflets, and the use of PTFE artificial chordae tendineae when excessive chordal elongation or rupture due to myxomatous degeneration co-exists. The aim of this review is to summarize the evolution of these techniques from the beginning till now.
- Published
- 2010
- Full Text
- View/download PDF
25. Cardiogenic shock as a complication of acute mitral valve regurgitation following posteromedial papillary muscle infarction in the absence of coronary artery disease
- Author
-
Frati Giacomo, Petrozza Vincenzo, Coluzzi Flaminia, Ricci Massimo, Mattia Consalvo, Bizzarri Federico, Pugliese Giuseppe, and Muzzi Luigi
- Subjects
Surgery ,RD1-811 ,Anesthesiology ,RD78.3-87.3 - Abstract
Abstract A 48 year old man was transferred to our department with cardiogenic shock, pyrexia, a high white cell count and significant serum troponin T level. Clinical evaluation revealed severe mitral regurgitation secondary to a flail of both mitral valve leaflets. An emergency cardiac catheterisation did not reveal any significant coronary artery disease. Left ventricular angiogram and echocardiography demonstrated a good left ventricular function and massive mitral regurgitation. Blood cultures were negative for aerobics, anaerobics and fungi. The patient underwent emergency mitral valve replacement with a mechanical valve. Intraoperatively, the posteromedial papillary muscle was found to be ruptured. Histology of the papillary muscle revealed myocardial necrosis with no signs of infection. Cultures obtained from a mitral valve specimen were negative. The patient's recovery was uneventful and he was discharged on the 6th postoperative day.
- Published
- 2008
- Full Text
- View/download PDF
26. Antegrade selective cerebral perfusion in patients with 'bovine aortic arch': is it easier?
- Author
-
Pagliaro Paolo, Frati Giacomo, Coluzzi Flaminia, Ricci Massimo, Di Marzio Emanuele, Di Nardo Matteo, Mattia Consalvo, Bizzarri Federico, Muzzi Luigi, and Petrozza Vincenzo
- Subjects
Surgery ,RD1-811 ,Anesthesiology ,RD78.3-87.3 - Published
- 2008
- Full Text
- View/download PDF
27. Traumatic aortic arch false aneurysm after blunt chest trauma in a motocross rider
- Author
-
Sartini Patrizio, Frati Giacomo, Pugliese Giuseppe, Muzzi Luigi, Rose David, Santo Chiara, Chirichilli Ilaria, Ricci Massimo, Mattia Consalvo, Bizzarri Federico, Ferrari Riccardo, Della Rocca Carlo, and Laghi Andrea
- Subjects
Surgery ,RD1-811 ,Anesthesiology ,RD78.3-87.3 - Abstract
Abstract This article details a case report of a traumatic aortic arch false aneurysm after blunt chest trauma. Thoracic aorta false aneurysms are a rare and life-threatening complication of aortic surgery, infection, genetic disorders and trauma.
- Published
- 2008
- Full Text
- View/download PDF
28. Core charge distribution and self assembly of columnar phases: the case of triphenylenes and azatriphenylenes
- Author
-
Berardi Roberto, Ricci Matteo, Muccioli Luca, Orlandi Silvia, and Zannoni Claudio
- Subjects
Chemistry ,QD1-999 - Abstract
Abstract Background The relation betweeen the structure of discotic molecules and columnar properties, a crucial point for the realization of new advanced materials, is still largely unknown. A paradigmatic case is that hexa-alkyl-thio substituted triphenylenes present mesogenic behavior while the corresponding azatriphenylenes, similar in shape and chemical structure, but with a different core charge distribution, do not form any liquid crystalline mesophase. This study is aimed at investigating, with the help of computer simulations techniques, the effects on phase behaviour of changes of the charge distribution in the discotic core. Results We described the shape and the pair, dispersive and electrostatic, interactions of hexa alkyl triphenylenes by uniaxial Gay-Berne discs with embedded point charges. Gay-Berne parameters were deduced by fitting the dispersive energies obtained from an atomistic molecular dynamics simulation of a small sample of hexa-octyl-thio triphenylene molecules in columnar phase, while a genetic algorithm was used to get a minimal set of point charges that properly reproduces the ab anitio electrostatic potential. We performed Monte Carlo simulations of three molecular models: the pure Gay-Berne disc, used as a reference, the Gay-Berne disc with hexa-thio triphenylene point charges, the Gay-Berne disc with hexa-thio azatriphenylene point charges. The phase diagram of the pure model evidences a rich polymorphism, with isotropic, columnar and crystalline phases at low pressure, and the appearance of nematic phase at higher pressure. Conclusion We found that the intermolecular electrostatic potential among the cores is fundamental in sta-bilizing/destabilizing columnar phases; in particular the triphenylene charge distribution stabilizes the columnar structure, while the azatriphenylene distribution suppresses its formation in favor of the nematic phase. We believe the present model could be successfully employed as the basis for coarse-grained level simulations of a wider class of triphenylene derivatives.
- Published
- 2007
- Full Text
- View/download PDF
29. Interaction of trehalose and glucose with a peptide beta-turn in aqueous solution
- Author
-
Oliver Alderman, Michael Di Gioacchino, Maria Antonietta RICCI, Fabio Bruni, Di Gioacchino, M, Bruni, F, Alderman, Olg, and Ricci, Ma
- Subjects
Glucose ,Materials Chemistry ,beta-turn ,Glycyl-L-prolyl-glycinamide (GPG) ,Trehalose ,Physical and Theoretical Chemistry ,Condensed Matter Physics ,Spectroscopy ,Atomic and Molecular Physics, and Optics ,Neutron diffraction ,Electronic, Optical and Magnetic Materials - Abstract
The issue of the interaction of carbohydrates with a peptide beta-turn is tackled by investigating at the atomic scale the interaction of glucose and trehalose with an aqueous solution of Glycyl-L-prolyl-glyci namide center dot HCl. The study, performed by neutron diffraction with isotopic substitution augmented by Monte Carlo simulation analysis, evidences clear differences between the two systems. Indeed, while trehalose confines water within the hydration shell of the solute, glucose enters this shell, in competition with water molecules. As a result, the structure of beta-turns in aqueous solution is stretched in presence of glucose as co-solute. This observations may be the key to distinguish the protection mechanism against drought from that against osmotic pressure. (C) 2022 Elsevier B.V. All rights reserved.
- Published
- 2022
30. Water in confined geometries
- Author
-
Nigro V., Bruni F., Ricci M. A., Debenedetti PG, Ricci MA, Bruni F, Nigro, V., Bruni, F., and Ricci, M. A.
- Abstract
Studies of water in confined geometries are of great relevance, since most water on the Earth surface is confined, in rocks, cells, food and so on. It is however yet unknown to what extent present knowledge about bulk water can help in understanding confined water behavior and vice versa, and to what extent the physics and chemistry of the confining medium affect the properties of bulk water. In other words, if there are any characteristics common to all confined waters. Here we present a structural study of water under several quite distinct confining media, showing that independently of the nature and shape of the substrate, the first water layers in the vicinity of the substrate surface are denser than those far away from it and that the average water density under confinement is lower than in bulk at the same temperature and pressure conditions. On the other hand, the extent of the structural disturbance to water structure, due to confinement, seems the result of a complex interplay between interaction forces and size.
- Published
- 2015
31. Water-Peptide Site-Specific Interactions: A Structural Study on the Hydration of Glutathione
- Author
-
Maria Antonietta Ricci, Fabio Bruni, Sylvia E. McLain, Ernesto Scoppola, Armida Sodo, E., Scoppola, Sodo, Armida, Se, Mclain, Ricci, Maria Antonietta, Bruni, Fabio, Scoppola, E, Mclain, Se, and Ricci, Ma
- Subjects
Models, Molecular ,aqueous solutions ,Stereochemistry ,Biophysics ,Glutamic Acid ,Tripeptide ,chemistry.chemical_compound ,neutron diffraction ,Organic chemistry ,Amines ,glutathione ,chemistry.chemical_classification ,Hydrogen bond ,Water ,Glutathione ,Amino acid ,Oxygen ,Solvation shell ,chemistry ,Thiol ,Solvents ,Glutathione disulfide ,Peptides ,Proteins and Nucleic Acids ,Cysteine - Abstract
Water-peptide interactions play an important role in determining peptide structure and function. Nevertheless, a microscopic description of these interactions is still incomplete. In this study we have investigated at the atomic scale length the interaction between water and the tripeptide glutathione. The rationale behind this work, based on the combination between a neutron diffraction experiment and a computer simulation, is twofold. It extends previous studies on amino acids, addressing issues such as the perturbation of the water network brought by a larger biomolecule in solution. In addition, and more importantly, it seeks a possible link between the atomic length scale description of the glutathione-water interaction with the specific biological functionality of glutathione, an important intracellular antioxidant. Results indicate a rather weak hydrogen bond between the thiol (-SH) group of cysteine and its first neighbor water molecule. This -SH group serves as a proton donor, is responsible for the biological activity of glutathione, and it is involved in the formation of glutathione disulfide, the oxidized form of glutathione. Moreover, the hydration shell of the chemically identical carboxylate group on the glutamic acid residue and on the glycine residue shows an intriguing different spatial location of water molecules and coordination numbers around the two View the MathML source groups.
- Published
- 2014
32. Study of percolation and clustering in supercritical water-CO2 mixtures
- Author
-
R. Mancinelli, Maria Antonietta Ricci, A. K. Soper, Roberto Triolo, A. Botti, Francesco Ferrante, Fabio Bruni, F. Lo Celso, BOTTI A, BRUNI F, MANCINELLI R, RICCI MA, LO CELSO F, TRIOLO R, FERRANTE F, SOPER AK, A., Botti, Bruni, Fabio, R., Mancinelli, Ricci, Maria Antonietta, F., LO CELSO, R., Triolo, F., Ferrante, and A. K., Soper
- Subjects
Chemistry ,Hydrogen bond ,Neutron diffraction ,Solvation ,General Physics and Astronomy ,Thermodynamics ,Percolation threshold ,supercritical water ,Supercritical fluid ,MD simulations, water, carbon dioxide ,Solvation shell ,neutron diffraction ,Percolation ,Physical chemistry ,Physical and Theoretical Chemistry ,Physics::Atmospheric and Oceanic Physics ,Phase diagram - Abstract
The microscopic structure of supercritical water-CO(2) mixture is investigated by neutron diffraction experiments exploiting the isotopic HD substitution. The investigated water reach mixtures are in the liquidlike region of the phase diagram, according to the behavior of the radial distribution functions, yet a reduction of the average number of hydrogen bonds, compared to equivalent states of pure water, is found. As a consequence, the average dimension of water clusters is reduced and the system stays below the percolation threshold. These results, along with the shift of the main peaks of the site-site radial distribution functions, suggest that the excess volume in these supercritical mixtures is likely associated with the CO(2) solvation shell.
- Published
- 2008
33. Dynamical propteries of comfined water upon supercooling
- Author
-
GALLO, PAOLA, Rovere M., Nardone M, Ricci MA, Gallo, Paola, and Rovere, M.
- Published
- 2001
34. Could Raman spectroscopy investigate the changes of cell oxidative stress status in thyroid diseases? A pilot study on cytological samples.
- Author
-
Di Gioacchino M, Verri M, Naciu AM, Paolucci A, di Masi A, Taffon C, Palermo A, Crescenzi A, Ricci MA, and Sodo A
- Abstract
The incidence of thyroid nodules is rapidly increasing worldwide. Raman spectroscopy (RS) is a powerful label-free and non-invasive technique, successfully used for early stage diagnosis. Here, RS is proposed as a tool to investigate the thyroid disease, including neoplasms, through the study of cell oxidative stress (OS), which represents one of the main cancer risk factors. In this study, we enrolled 28 patients, submitted to a first and second thyroid fine needle aspiration (FNA) during follow up. The cytological samples were studied by RS and morphological examination. Typical Raman spectra of thyroid cytological samples are reported and the contribution of oxidized and reduced cytochrome b and c and carotenoids are discussed. On the basis of the evolution of the Raman features over the time lapse between the two FNAs, the 28 patients have been classified into 4 different categories and the most representative case for each category is reported and discussed in detail. For each category, the different Raman intensity ratio between oxidized and reduced cytochromes b and c is reported and associated to different cell OS status, along with the presence of carotenoids. Overall, our results support a correlation among changes in oxidative stress, carotenoids uptake and thyroid diseases, which could inspire new fundamental research on biomarkers and signaling pathways involved in thyroid OS., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
35. Unraveling the Effects of Carotenoids Accumulation in Human Papillary Thyroid Carcinoma.
- Author
-
di Masi A, Sessa RL, Cerrato Y, Pastore G, Guantario B, Ambra R, Di Gioacchino M, Sodo A, Verri M, Crucitti P, Longo F, Naciu AM, Palermo A, Taffon C, Acconcia F, Bianchi F, Ascenzi P, Ricci MA, and Crescenzi A
- Abstract
Among the thyroid cancers, papillary thyroid cancer (PTC) accounts for 90% of the cases. In addition to the necessity to identify new targets for PTC treatment, early diagnosis and management are highly demanded. Previous data indicated that the multivariate statistical analysis of the Raman spectra allows the discrimination of healthy tissues from PTC ones; this is characterized by bands typical of carotenoids. Here, we dissected the molecular effects of carotenoid accumulation in PTC patients by analyzing whether they were required to provide increased retinoic acid (RA) synthesis and signaling and/or to sustain antioxidant functions. HPLC analysis revealed the lack of a significant difference in the overall content of carotenoids. For this reason, we wondered whether the carotenoid accumulation in PTC patients could be related to vitamin A derivative retinoic acid (RA) biosynthesis and, consequently, the RA-related pathway activation. The transcriptomic analysis performed using a dedicated PCR array revealed a significant downregulation of RA-related pathways in PTCs, suggesting that the carotenoid accumulation in PTC could be related to a lower metabolic conversion into RA compared to that of healthy tissues. In addition, the gene expression profile of 474 PTC cases previously published in the framework of the Cancer Genome Atlas (TGCA) project was examined by hierarchical clustering and heatmap analyses. This metanalysis study indicated that the RA-related pathways resulted in being significantly downregulated in PTCs and being associated with the follicular variant of PTC (FV-PTC). To assess whether the possible fate of the carotenoids accumulated in PTCs is associated with the oxidative stress response, the expression of enzymes involved in ROS scavenging was checked. An increased oxidative stress status and a reduced antioxidant defense response were observed in PTCs compared to matched healthy thyroids; this was possibly associated with the prooxidant effects of high levels of carotenoids. Finally, the DepMap datasets were used to profile the levels of 225 metabolites in 12 thyroid cancer cell lines. The results obtained suggested that the high carotenoid content in PTCs correlates with tryptophan metabolism. This pilot provided novel possible markers and possible therapeutic targets for PTC diagnosis and therapy. For the future, a larger study including a higher number of PTC patients will be necessary to further validate the molecular data reported here.
- Published
- 2022
- Full Text
- View/download PDF
36. Mapping at the nanometer scale the effects of sea-salt derived chlorine on cinnabar and lead white by using delayed image extraction in ToF-SIMS.
- Author
-
Iorio M, Sodo A, Graziani V, Branchini P, Casanova Municchia A, Ricci MA, Salvadori O, Fiorin E, and Tortora L
- Abstract
In this work, an innovative analytical approach focused on the use of advanced imaging techniques for the chemical mapping of degradation and/or restoration products is proposed. A representative cross-section showing a very complex stratigraphy from the Saint Wilgefortis Triptych (Hieronymus Bosch), exhibited in the Galleria dell'Accademia di Venezia, was investigated. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) experiments were performed using a time-of-flight detector operating in the so-called delayed extraction mode. The time delay applied during the extraction of the secondary ions permitted mass spectra to be obtained with an excellent mass resolution and chemical maps with nanometer scale spatial resolution. The painting's cross-section was also analysed at the micrometer scale by micro-Fourier transform infrared spectroscopy (micro-FTIR). The combined analytical approaches highlighted the colocalization of lead chloride, oxychloride, and hydroxychloride ions, suggesting the transformation of lead white ((PbCO
3 )2 Pb(OH)2 ) into laurionite (PbClOH). Furthermore, chlorine appears evenly diffused in the cinnabar (HgS) layer, inducing the alteration of its more external part into calomel (Hg2 Cl2 ). In fact, from the chemical maps the presence in the sample of an unaltered portion of the cinnabar layer is evident. Such degradation products were probably due to the exposure of the painting to a chloride-rich atmosphere for a long time. This led to a global blackening of the painting. To protect the painting from aggressive chemical species, siloxane compounds were probably used as a modern restorative treatment. ToF-SIMS chemical maps revealed permeation of the silicon-based consolidants within the sample's cracks and no interaction products with the other constitutive materials of the painting were found. Finally, the presence of different lead soaps was detected in correspondence with the lead white layer.- Published
- 2021
- Full Text
- View/download PDF
37. Circulating Levels of Sclerostin Predict Glycemic Improvement after Sleeve Gastrectomy.
- Author
-
Carbone F, Nulli Migliola E, Bonaventura A, Vecchié A, De Vuono S, Ricci MA, Vaudo G, Boni M, Ministrini S, Lupattelli G, and Montecucco F
- Subjects
- Adult, Anthropometry, Blood Glucose metabolism, Bone Remodeling, Cohort Studies, Female, Humans, Insulin Resistance, Laparoscopy methods, Male, Middle Aged, Obesity, Morbid surgery, Postoperative Period, Predictive Value of Tests, Treatment Outcome, Adaptor Proteins, Signal Transducing blood, Gastrectomy methods, Homeostasis physiology, Insulin blood, Obesity, Morbid blood
- Abstract
Among the different effects of bariatric surgery, here we focus on bone-derived inflammatory molecules, and in particular, sclerostin; an osteocyte product potentially associated with cardio-metabolic diseases. In 94 morbidly obese patients undergoing laparoscopic sleeve gastrectomy (SG), over-time changes in anthropometric and biochemical measures-including insulin resistance (IR) indexes-were correlated with serum sclerostin levels. Sclerostin was positively associated with anthropometric indexes of obesity, and inversely with IR, namely homeostatic model assessment for peripheral insulin sensitivity (HOMA2%S) (r = -0.218; p = 0.045). Sclerostin emerged as the only significant predictor of HOMA2-%S normalization, independently of demographic and anthropometric variables (OR 1.01 (95% CI 1.00-1.02); p = 0.024). We also identified two distinct patterns of serum sclerostin change: the higher/lower sclerostin levels at baseline, the greater their post-surgical reduction/increase ( p < 0.001 for all subgroups). Among those two patterns, especially the post-surgery increase in serum sclerostin was associated with lean mass reduction, without any association with IR indexes. Although counterintuitive, this change was likely dependent on the post-surgical increase in bone turnover. In conclusion, baseline serum levels of sclerostin correlate with anthropometric measures of obesity and IR, and the ability to predict glycemic improvements after SG. Specifically, serum sclerostin was closely associated with peripheral insulin sensitivity (HOMA2-%S), thus supporting the role of skeletal muscle/bone interactions in metabolic diseases.
- Published
- 2021
- Full Text
- View/download PDF
38. Raman Spectroscopy Discloses Altered Molecular Profile in Thyroid Adenomas.
- Author
-
Sodo A, Verri M, Palermo A, Naciu AM, Sponziello M, Durante C, Di Gioacchino M, Paolucci A, di Masi A, Longo F, Crucitti P, Taffon C, Ricci MA, and Crescenzi A
- Abstract
Follicular patterned nodules are sometimes complex to be classified due to ambiguous nuclear features and/or questionable capsular or vascular invasion. In this setting, there is a poor inter-observer concordance even among expert pathologists. Raman spectroscopy was recently used to separate benign and malignant thyroid nodules based on their molecular fingerprint; anyway, some histologically proved follicular adenomas were clustered as having a characteristic profile of malignant lesions. In this study, we analyzed five follicular thyroid adenomas with a malignant spectroscopic profile compared to five follicular adenomas with a benign Raman spectrum in order to assess possible molecular differences between the two groups. Morphological, immunohistochemical, and molecular analyses evidenced expression of malignancy-associated proteins in four out of five malignant clustered adenomas. The remaining malignant clustered adenoma showed a TSHR mutation previously associated with autonomously functioning follicular carcinomas. In conclusion, thyroid follicular adenomas are a group of morphologically benign neoplasms that may have altered the mutational or expression profile; cases of adenomas with altered immunophenotype are recognized as showing a profile associated with malignancy by Raman spectroscopy. This correlation warrants a more extensive evaluation and suggests a potential predictive value of spectroscopic assessment in recognizing characteristics associated with tumor progression in follicular thyroid neoplasms.
- Published
- 2020
- Full Text
- View/download PDF
39. Magnetic mesoporous silica nanostructures: investigation of magnetic properties.
- Author
-
Talone A, Ruggiero L, Slimani S, Imperatori P, Barucca G, Ricci MA, Sodo A, and Peddis D
- Abstract
Magnetic mesoporous silica (MS) nanocomposites provide the possibility of generating multi-functional objects for application in different technological areas. This paper focuses on the magnetic properties of nanocomposites constituted by spinel iron oxide nanoparticles (magnetic nanoparticles (MNPs), < D > ≈ 8-9 nm) embedded in an MS matrix. The mesoporous structure of the silica matrix and the presence of the nanoparticles inside clearly emerge from transmission electron microscopy (TEM) measurements. Low temperature (5 K) field-dependent magnetization measurements reveal saturation magnetization (M
S ) close to bulk value (MS bulk ∼ 90 emu g-1 ) for both MNPs and MNP/MS nanocomposites, indicating that the presence of silica does not affect the magnetic features of the single MNPs. Moreover, the dependence of the remanent magnetization on field (i.e. δM plots) at low temperature has shown a small but evident decrease of interaction in an MNP/MS sample with respect to MNP samples A m2 Kg-1 . Finally, a partial orientation of the easy axis is observed when the MNPs are embedded in the silica matrix.- Published
- 2020
- Full Text
- View/download PDF
40. Thyroid cancer diagnosis by Raman spectroscopy.
- Author
-
Sbroscia M, Di Gioacchino M, Ascenzi P, Crucitti P, di Masi A, Giovannoni I, Longo F, Mariotti D, Naciu AM, Palermo A, Taffon C, Verri M, Sodo A, Crescenzi A, and Ricci MA
- Subjects
- Adenoma diagnosis, Adenoma pathology, Biopsy, Fine-Needle methods, Diagnosis, Differential, Female, Humans, Male, Middle Aged, Spectrum Analysis, Raman methods, Thyroid Gland pathology, Thyroid Neoplasms pathology, Thyroid Nodule diagnosis, Thyroid Nodule pathology, Thyroid Neoplasms diagnosis
- Abstract
Over the last 50 years, the incidence of human thyroid cancer disease has seen a significative increment. This comes along with an even higher increment of surgery, since, according to the international guidelines, patients are sometimes addressed to surgery also when the fine needle aspiration gives undetermined cytological diagnosis. As a matter of fact, only 30% of the thyroid glands removed for diagnostic purpose have a post surgical histological report of malignancy: this implies that about 70% of the patients have suffered an unnecessary thyroid removal. Here we show that Raman spectroscopy investigation of thyroid tissues provides reliable cancer diagnosis. Healthy tissues are consistently distinguished from cancerous ones with an accuracy of [Formula: see text] 90%, and the three cancer typology with highest incidence are clearly identified. More importantly, Raman investigation has evidenced alterations suggesting an early stage of transition of adenoma tissues into cancerous ones. These results suggest that Raman spectroscopy may overcome the limits of current diagnostic tools.
- Published
- 2020
- Full Text
- View/download PDF
41. Determinants of High Parathyroid Hormone Levels in Patients With Severe Obesity and Their Relationship With the Cardiometabolic Risk Factors, Before and After a Laparoscopic Sleeve Gastrectomy Intervention.
- Author
-
Ministrini S, Ricci MA, Daviddi G, Scavizzi M, De Vuono S, D'Abbondanza M, Paganelli MT, Boni M, Roscini AR, Scarponi AM, Vaudo G, and Lupattelli G
- Subjects
- Cardiometabolic Risk Factors, Carotid Intima-Media Thickness, Gastrectomy, Humans, Parathyroid Hormone, Vitamin D, Laparoscopy, Obesity, Morbid surgery, Vitamin D Deficiency complications
- Abstract
Background: Obesity is a risk factor for vitamin D deficiency and hyperparathyroidism. Hyperparathyroidism could exert a negative effect on glucose metabolism and vascular function. The aim of this study was to identify the determinants of hyperparathyroidism beyond vitamin D deficiency, whether hyperparathyroidism could have a negative impact on individual health and whether laparoscopic sleeve gastrectomy (LSG) negatively affects the levels of intact parathyroid hormone (iPTH) and 25(OH) vitamin D (25(OH)D)., Methods: We evaluated the levels of iPTH, 25(OH)D, and leptin, together with markers of insulin sensitivity and early cardiovascular disease, in a cohort of 160 patients with severe obesity before and after an LSG intervention., Results: Ninety-seven percent of subjects had vitamin D deficiency, and 72% of them had hyperparathyroidism. After correcting for possible confounders, we found a correlation between iPTH levels and carotid intima-media thickness, as well as with the HOMA index. After the LSG, 25(OH)D levels were significantly increased, while iPTH levels were significantly reduced. The reduction of iPTH was significantly correlated with the reduction of BMI, diastolic blood pressure, and leptin, which was the independent predictor of iPTH reduction., Conclusions: Our results suggest that vitamin D deficiency is not the sole determinant of hyperparathyroidism in severe obesity because visceral fat deposition and leptin could both play a role. Obesity-related hyperparathyroidism is associated with insulin resistance and atherosclerosis, although the results from previous studies were conflicting. Finally, LSG intervention does not negatively affect vitamin D status and improves hyperparathyroidism.
- Published
- 2020
- Full Text
- View/download PDF
42. Folate and Vitamin B12 in Morbid Obesity: The Influence of Folate on Anti-Atherogenic Lipid Profile.
- Author
-
Daviddi G, Ricci MA, De Vuono S, Gentili A, Boni M, and Lupattelli G
- Subjects
- Folic Acid chemistry, Humans, Vitamins metabolism, Folic Acid metabolism, Lipids chemistry, Obesity, Morbid, Vitamin B 12 metabolism, Vitamins chemistry
- Abstract
Previous studies showed a high prevalence of micronutrient deficiencies in obese subjects, with low folate and vitamin B12 serum levels and intakes. Correlations between vitamins and lipids have been investigated both in animal and human studies. The aim of our study is to evaluate the influence of dietetic and serum levels of folate and vitamin B12 on lipid pattern in morbidly obese subjects. We also analysed the relationship between serum concentrations and dietary intake of these micronutrients, and compared the intakes to the Recommended Levels of Nutrients and Energy Intakes (LARN). In 122 morbidly obese patients, mean BMI 45 ± 7 kg/m
2 , we evaluated anthropometric parameters, hepatic, glyco/lipid profile, total folate and vitamin B12, blood pressure, and finally nutritional intakes in a subgroup of 68 patients using a food frequency questionnaire about the frequency of food consumption and daily water intake. These values were determined in obese patients before and one year after sleeve gastrectomy. Both before and after surgery, levels of vitamins and minerals remained in normal range compared to LARN. According to univariate analysis, at baseline folate showed a significantly positive correlation with high-density lipoprotein cholesterol (p = 0.028, ρ = 0.204), apolipoprotein A-I (p = 0.006, ρ = 0.268) and vitamin B12 (p = 0.040, ρ = 0.192), and a significantly negative correlation with triglycerides (p = 0.049, ρ = -0.184). Folate and vitamin B12 levels do not correlate with their nutritional intakes, which remain within recommended range after surgery. In conclusion the correlation between folate and anti-atherogenic lipid profile is confirmed also in a large group of morbid obese patients.- Published
- 2020
- Full Text
- View/download PDF
43. Hydration of Carboxyl Groups: A Route toward Molecular Recognition?
- Author
-
Di Gioacchino M, Bruni F, Imberti S, and Ricci MA
- Subjects
- Computer Simulation, Hydrophobic and Hydrophilic Interactions, Protons, Neutron Diffraction, Water
- Abstract
On Earth, water plays an active role in cellular life, over several scales of distance and time. At a nanoscale, water drives macromolecular conformation through hydrophobic forces and at short times acts as a proton donor/acceptor providing charge carriers for signal transmission. At longer times and larger distances, water controls osmosis, transport, and protein mobility. Neutron diffraction experiments augmented by computer simulation, show that the three-dimensional shape of the hydration shell of carboxyl and carboxylate groups belonging to different molecules is characteristic of each molecule. Different hydration shells identify and distinguish specific sites with the same chemical structure. This experimental evidence suggests an active role of water also in controlling, modulating, and mediating chemical reactions involving carboxyl and carboxylate groups.
- Published
- 2020
- Full Text
- View/download PDF
44. Chemerin predicts carotid intima-media thickening in severe obesity.
- Author
-
Ministrini S, Ricci MA, Nulli Migliola E, De Vuono S, D'Abbondanza M, Paganelli MT, Vaudo G, Siepi D, and Lupattelli G
- Abstract
Background and Aims: Chemerin is an adipokine with an emerging role in the crosstalk between adipose tissue and immune system. It is overexpressed in severe obesity, affects adipogenesis and glucose homeostasis and it correlates with early vascular damage. The aim of this study is to investigate the correlation between circulating levels of chemerin and early vascular damage in subjects with severe obesity, before and after laparoscopic sleeve gastrectomy (LSG)., Methods: Fifty-six obese subjects eligible for LSG were enrolled in the study. The following parameters were evaluated: body mass index (BMI), glycemia, insulinemia, glycated haemoglobin, lipid profile, plasma chemerin levels and carotid intima-media thickness (cIMT). Fifty-four subjects were evaluated 1 year after the intervention., Results: Univariate analysis showed a direct and significant correlation between chemerin and waist circumference, insulin resistance, glycated haemoglobin and cIMT. Chemerin was a better predictor of intima-media thickening than waist circumference and glycated haemoglobin at the ROC curve analysis, with a cut-off value for chemerin of 140 ng/mL. The reduction of chemerin is independently associated with the reduction of cIMT and the improvement of insulin sensitivity after LSG., Conclusion: Chemerin is involved in the development and progression of early vascular damage and insulin resistance in subjects with severe obesity, and in their healing after bariatric surgery. Chemerin could also have a role in the assessment of cardiovascular risk in subjects with severe obesity., (© 2020 Stichting European Society for Clinical Investigation Journal Foundation.)
- Published
- 2020
- Full Text
- View/download PDF
45. Metabolic profile of human parathyroid adenoma.
- Author
-
di Masi A, Leboffe L, Sodo A, Tabacco G, Cesareo R, Sbroscia M, Giovannoni I, Taffon C, Crucitti P, Longo F, Manfrini S, Ricci MA, Ascenzi P, Crescenzi A, and Palermo A
- Subjects
- Female, Humans, Metabolome, Parathyroid Glands, Parathyroid Hormone, Parathyroidectomy, Adenoma surgery, Parathyroid Neoplasms surgery
- Abstract
Purpose: Recently, it has been demonstrated that Raman spectroscopy is able to differentiate between healthy parathyroid tissues and parathyroid adenoma based on the basis of a specific molecular fingerprint. However, to our knowledge, no previous studies have been performed to evaluate the metabolic profile of parathyroid adenoma. Therefore, we designed a proof of concept study aimed to investigate the glucose/fatty acid metabolisms, in addition to the mitochondrial changes, in solitary parathyroid adenoma and in healthy parathyroid glands., Methods: Nine females with primary hyperparathyroidism due to a solitary parathyroid adenoma and formal surgical indication for parathyroidectomy have been enrolled. At the time of surgery, the removed specimens were immediately submitted unfixed and a tissue slice of about 0.5 cm in diameter was obtained from the nodular lesion. The expression of selected metabolic enzymes and proteins has been evaluated by western blot analysis, using human parathyroid whole tissue lysates as control., Results: Data obtained highlighted an increase, compared with the healthy group, of: (i) the glucose uptake by the GLUT-1 receptor and its phosphorylation by hexokinase II (HXKII); (ii) the expression of 3-phosphoglycerate dehydrogenase (3-PGDH) and glucose-6-phosphate dehydrogenase (G6PD); (iii) lipids biosynthesis; and (iv) cytochrome c expression., Conclusions: Our findings highlight for the first time the parathyroid adenoma metabolic hallmarks that could represent potential molecular targets usable for the development of new pharmacological treatments, allowing to reduce surgical parathyroidectomy.
- Published
- 2020
- Full Text
- View/download PDF
46. Role of Water in Sucrose, Lactose, and Sucralose Taste: The Sweeter, The Wetter?
- Author
-
Imberti S, McLain SE, Rhys NH, Bruni F, and Ricci MA
- Abstract
Natural sugars combine energy supply and, except a few cases, a pleasant taste. On the other hand, exaggerated consumption may impact population health. This has busted the research for the synthesis of increasingly cheaper artificial sweeteners, with low energy content and intense taste. Here, we suggest that studies of the hydration properties of three disaccharides, namely, the natural sucrose and lactose and the artificial sucralose, may explain the difference by orders of magnitude among their sweetness. This is done by analyzing via Monte Carlo simulations the neutron diffraction differential cross sections of aqueous solutions of the three sugars and their isotopes. Our results show that the strength of the sugar-water hydrogen bond interaction is one of the factors influencing sweetness, another being the number of water molecules within the first neighboring shell of the sugar whether bonded or not., Competing Interests: The authors declare no competing financial interest., (Copyright © 2019 American Chemical Society.)
- Published
- 2019
- Full Text
- View/download PDF
47. Increased plasmatic NETs by-products in patients in severe obesity.
- Author
-
D'Abbondanza M, Martorelli EE, Ricci MA, De Vuono S, Migliola EN, Godino C, Corradetti S, Siepi D, Paganelli MT, Maugeri N, and Lupattelli G
- Subjects
- Adult, Aged, Anthropometry, Bariatric Surgery, Blood Glucose, Blood Pressure physiology, Female, Humans, Intra-Abdominal Fat physiopathology, Intra-Abdominal Fat surgery, Male, Middle Aged, Obesity, Morbid pathology, Obesity, Morbid surgery, Extracellular Traps metabolism, Intra-Abdominal Fat metabolism, Obesity, Morbid blood, Weight Loss
- Abstract
Neutrophil extracellular traps (NETs) are DNAs products involved in immune process. Obesity through a low-grade chronic inflammation determines neutrophil activation, but it is still unclear its role in NETs formation. Here we analyzed the NETs levels in healthy and morbid obese, their association with anthropometric and glyco-metabolic parameters and their changes after bariatric surgery. For this study, we enrolled 73 patients with morbid obesity (BMI ≥40 kg/m
2 or ≥35 kg/m2 + comorbidity) eligible to sleeve gastrectomy. In parallel, 55 healthy subjects and 21 patients with severe coronary artery disease were studied as controls. We evaluated anthropometric parameters, peripheral blood pressure, biochemical and serum analysis at the enrollment and at twelve months after surgery. Plasmatic levels of MPO-DNA complexes were assessed by ELISA. NETs levels were higher in obese than in control group (p < 0.001) and correlated with the main anthropometric variable (BMI, waist, hip), glyco-metabolic variables and systolic blood pressure. NETs trend after intervention was uneven. The reduction of NETs correlated with the entity of reduction of BMI (ρ = 0.416, p < 0.05), visceral fat area (ρ = 0.351, p < 0.05), and glycemia (ρ = 0.495, p < 0.001). In medical history of patients in whom NETs increased, we observed a higher number of thromboembolic events. Our observations indicate that severe obesity is associated with increased generation of NETs, which in turn could influence the patients' systemic inflammatory state. Weight loss and in particular, loss of adipose tissue after bariatric surgery does not in itself correct NET's dysregulated production. Finally, patients in whom NETs accumulation persists after surgery are probably those at the highest risk of cardiovascular events.- Published
- 2019
- Full Text
- View/download PDF
48. Serum Bile Acid Levels Before and After Sleeve Gastrectomy and Their Correlation with Obesity-Related Comorbidities.
- Author
-
De Vuono S, Ricci MA, Nulli Migliola E, Monti MC, Morretta E, Boni M, Ministrini S, Carino A, Fiorucci S, Distrutti E, and Lupattelli G
- Subjects
- Adult, Bariatric Surgery methods, Blood Glucose metabolism, Comorbidity, Female, Glycated Hemoglobin analysis, Humans, Insulin blood, Insulin Resistance physiology, Intra-Abdominal Fat metabolism, Lipids blood, Male, Middle Aged, Obesity, Morbid complications, Obesity, Morbid epidemiology, Retrospective Studies, Bile Acids and Salts blood, Gastrectomy methods, Obesity, Morbid blood, Obesity, Morbid surgery
- Abstract
Background and Aims: The rising prevalence of morbid obesity is increasing the demand for bariatric surgery. The benefits observed after bariatric surgery seems to be not fully explained by surgery-induced weight loss or traditional cardiovascular risk factors regression or improvement. Some evidences suggest that bile acid (BA) levels change after bariatric surgery, thus suggesting that BA concentrations could influence some of the metabolic improvement induced by bariatric surgery. In this report, we have characterized circulating BA patterns and compared them to metabolic and vascular parameters before and after sleeve gastrectomy (SG)., Patients and Methods: Seventy-nine subjects (27 males, 52 females, aged 45 ± 12 years, mean BMI 45 ± 7 kg/m
2 ) SG candidates were included in the study. Before and about 12 months after SG, all subjects underwent a clinical examination, blood tests (including lipid profile, plasma glucose and insulin, both used for calculating HOMA-IR, and glycated hemoglobin), ultrasound visceral fat area estimation, ultrasound flow-mediated dilation evaluation, and determination of plasma BA concentrations., Results: Before SG, both primary and secondary BA levels were higher in insulin-resistant obese subjects than in non-insulin resistant obese, and BA were positively associated with the markers of insulin-resistance. After SG, total (conjugated and unconjugated) cholic acids significantly decreased (p 0.007), and total lithocholic acids significantly increased (p 0.017). SG-induced total cholic and chenodeoxycholic acid changes were directly associated with surgery-induced glycemia (p 0.011 and 0.033 respectively) and HOMA-IR (p 0.016 and 0.012 respectively) changes., Conclusions: Serum BA are associated with glucose metabolism and particularly with markers of insulin-resistance. SG modifies circulating BA pool size and composition. SG-induced BA changes are associated with insulin-resistance amelioration. In conclusion, an interplay between glucose metabolism and circulating BA exists but further studies are needed.- Published
- 2019
- Full Text
- View/download PDF
49. Vibrational dynamics of confined supercooled water.
- Author
-
Stefanutti E, Bove LE, Alabarse FG, Lelong G, Bruni F, and Ricci MA
- Abstract
The quest for a possible liquid-liquid coexistence line in supercooled water below its homogeneous nucleation temperature is faced by confining water within a porous silica substrate (MCM-41). This system is investigated by synchrotron radiation infrared spectroscopy, exploring both the intramolecular and the intermolecular vibrational dynamics, in the temperature range from ambient down to ∼120 K, along several isobaric paths between 0.7 kbar and 3.0 kbar. Upon lowering the temperature, the OH-stretching band shows that the intramolecular vibrational dynamics continuously evolves from predominantly liquidlike to predominantly icelike. An abrupt change in the line shape of the intermolecular vibrational band between 220 K and 240 K, depending on the pressure, is the signature of nucleation of ice within the MCM-41 pores. These findings do not support the presence of two liquid phases and provide evidence for the coexistence of liquid water and ice in water confined in MCM-41.
- Published
- 2019
- Full Text
- View/download PDF
50. Lysosomal Acid Lipase as a Molecular Target of the Very Low Carbohydrate Ketogenic Diet in Morbidly Obese Patients: The Potential Effects on Liver Steatosis and Cardiovascular Risk Factors.
- Author
-
Ministrini S, Calzini L, Nulli Migliola E, Ricci MA, Roscini AR, Siepi D, Tozzi G, Daviddi G, Martorelli EE, Paganelli MT, and Lupattelli G
- Abstract
A very low carbohydrate ketogenic diet (VLCKD) is an emerging technique to induce a significant, well-tolerated, and rapid loss of body weight in morbidly obese patients. The low activity of lysosomal acid lipase (LAL) could be involved in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), which is a common feature in morbidly obese patients. Fifty-two obese patients suitable for a bariatric surgery intervention underwent a 25-day-long VLCKD. The biochemical markers of glucose and lipid metabolism, and flow-mediated dilation (FMD) of the brachial artery were measured before and after VLCKD. LAL activity was measured using the dried blood spot technique in 20 obese patients and in a control group of 20 healthy, normal-weight subjects. After VLCKD, we observed a significant reduction in body mass index, fasting glucose, insulinemia, and lipid profile parameters. No significant variation in FMD was observed. The number of patients with severe liver steatosis significantly decreased. LAL activity significantly increased, although the levels were not significantly different as compared to the control group. In conclusion, VLCKD induces the activity of LAL in morbidly obese subjects and reduces the secretion of all circulating lipoproteins. These effects could be attributed to the peculiar composition of the diet, which is particularly poor in carbohydrates and relatively rich in proteins.
- Published
- 2019
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.