Search

Your search keyword '"Riccardo, Risorti"' showing total 3 results
Start Over Author "Riccardo, Risorti"
3 results on '"Riccardo, Risorti"'

2. An active site at work - the role of key residues in C. diphteriae coproheme decarboxylase

Author

Federico Sebastiani, Riccardo Risorti, Chiara Niccoli, Hanna Michlits, Maurizio Becucci, Stefan Hofbauer, and Giulietta Smulevich

Subjects
Inorganic Chemistry, Bacterial Proteins, Distal histidine, Fe-His strength, Hemin reconstitution, Prokaryotic heme biosynthesis, Resonance Raman, Site-directed variants, Carboxy-Lyases, Catalytic Domain, Corynebacterium diphtheriae, Mutation, Biocatalysis, Heme, Hydrogen Peroxide, Biochemistry
Abstract

Coproheme decarboxylases (ChdCs) are utilized by monoderm bacteria to produce heme b by a stepwise oxidative decarboxylation of the 2- and 4-propionate groups of iron coproporphyrin III (coproheme) to vinyl groups. This work compares the effect of hemin reconstitution versus the hydrogen peroxide-mediated conversion of coproheme to heme b in the actinobacterial ChdC from Corynebacterium diphtheriae (CdChdC) and selected variants. Both ferric and ferrous forms of wild-type (WT) CdChdC and its H118A, H118F, and A207E variants were characterized by resonance Raman and UV-vis spectroscopies. The heme b ligand assumes the same conformation in the WT active site for both the reconstituted and H

Published
2022

Catalog

Books, media, physical & digital resources
See catalog results