14 results on '"Ribichich KF"'
Search Results
2. Key role of the motor protein Kinesin 13B in the activity of homeodomain-leucine zipper I transcription factors.
- Author
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Miguel VN, Ribichich KF, Giacomelli JI, and Chan RL
- Subjects
- Gene Expression Regulation, Plant, Homeodomain Proteins genetics, Homeodomain Proteins metabolism, Kinesins genetics, Plant Proteins genetics, Plant Proteins metabolism, Leucine Zippers, Transcription Factors genetics, Transcription Factors metabolism
- Abstract
The sunflower (Helianthus annuus) homeodomain-leucine zipper I transcription factor HaHB11 conferred differential phenotypic features when it was expressed in Arabidopsis, alfalfa, and maize plants. Such differences were increased biomass, seed yield, and tolerance to flooding. To elucidate the molecular mechanisms leading to such traits and identify HaHB11-interacting proteins, a yeast two-hybrid screening of an Arabidopsis cDNA library was carried out using HaHB11 as bait. The sole protein identified with high confidence as interacting with HaHB11 was Kinesin 13B. The interaction was confirmed by bimolecular fluorescence complementation and by yeast two-hybrid assay. Kinesin 13B also interacted with AtHB7, the Arabidopsis closest ortholog of HaHB11. Histochemical analyses revealed an overlap between the expression patterns of the three genes in hypocotyls, apical meristems, young leaves, vascular tissue, axillary buds, cauline leaves, and cauline leaf nodes at different developmental stages. AtKinesin 13B mutants did not exhibit a differential phenotype when compared with controls; however, both HaHB11 and AtHB7 overexpressor plants lost, partially or totally, their differential phenotypic characteristics when crossed with such mutants. Altogether, the results indicated that Kinesin 13B is essential for the homeodomain-leucine zipper transcription factors I to exert their functions, probably via regulation of the intracellular distribution of these transcription factors by the motor protein., (© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
- Published
- 2020
- Full Text
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3. Successful field performance in warm and dry environments of soybean expressing the sunflower transcription factor HB4.
- Author
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Ribichich KF, Chiozza M, Ávalos-Britez S, Cabello JV, Arce AL, Watson G, Arias C, Portapila M, Trucco F, Otegui ME, and Chan RL
- Subjects
- Argentina, Droughts, Plants, Genetically Modified genetics, Glycine max genetics, Transcription Factors genetics, Arabidopsis genetics, Helianthus genetics
- Abstract
Soybean yield is limited primarily by abiotic constraints. No transgenic soybean with improved abiotic stress tolerance is commercially available. We transformed soybean plants with genetic constructs able to express the sunflower transcription factor HaHB4, which confers drought tolerance to Arabidopsis and wheat. One line (b10H) carrying the sunflower promoter was chosen among three independent lines because it exhibited the best performance in seed yield, and was evaluated in the greenhouse and in 27 field trials in different environments in Argentina. In greenhouse experiments, transgenic plants showed increased seed yield under stress conditions together with greater epicotyl diameter, larger xylem area, and increased water use efficiency compared with controls. They also exhibited enhanced seed yield in warm and dry field conditions. This response was accompanied by an increase in seed number that was not compensated by a decrease in individual seed weight. Transcriptome analysis of plants from a field trial with maximum difference in seed yield between genotypes indicated the induction of genes encoding redox and heat shock proteins in b10H. Collectively, our results indicate that soybeans transformed with HaHB4 are expected to have a reduced seed yield penalty when cultivated in warm and dry conditions, which constitute the best target environments for this technology., (© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.)
- Published
- 2020
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4. An Interdisciplinary Approach to Study the Performance of Second-generation Genetically Modified Crops in Field Trials: A Case Study With Soybean and Wheat Carrying the Sunflower HaHB4 Transcription Factor.
- Author
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González FG, Rigalli N, Miranda PV, Romagnoli M, Ribichich KF, Trucco F, Portapila M, Otegui ME, and Chan RL
- Abstract
Research, production, and use of genetically modified (GM) crops have split the world between supporters and opponents. Up to now, this technology has been limited to the control of weeds and pests, whereas the second generation of GM crops is expected to assist farmers in abiotic stress tolerance or improved nutritional features. Aiming to analyze this subject holistically, in this presentation we address an advanced technology for drought-tolerant GM crops, upscaling from molecular details obtained in the laboratory to an extensive network of field trials as well as the impact of the introduction of this innovation into the market. Sunflower has divergent transcription factors, which could be key actors in the drought response orchestrating several signal transduction pathways, generating an improved performance to deal with water deficit. One of such factors, HaHB4, belongs to the homeodomain-leucine zipper family and was first introduced in Arabidopsis. Transformed plants had improved tolerance to water deficits, through the inhibition of ethylene sensitivity and not by stomata closure. Wheat and soybean plants expressing the HaHB4 gene were obtained and cropped across a wide range of growing conditions exhibiting enhanced adaptation to drought-prone environments, the most important constraint affecting crop yield worldwide. The performance of wheat and soybean, however, differed slightly across mentioned environments; whereas the improved behavior of GM wheat respect to controls was less dependent on the temperature regime (cool or warm), differences between GM and wild-type soybeans were remarkably larger in warmer compared to cooler conditions. In both species, these GM crops are good candidates to become market products in the near future. In anticipation of consumers' and other stakeholders' interest, spectral analyses of field crops have been conducted to differentiate these GM crops from wild type and commercial cultivars. In this paper, the potential impact of the release of such market products is discussed, considering the perspectives of different stakeholders., (Copyright © 2020 González, Rigalli, Miranda, Romagnoli, Ribichich, Trucco, Portapila, Otegui and Chan.)
- Published
- 2020
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5. Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type.
- Author
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González FG, Capella M, Ribichich KF, Curín F, Giacomelli JI, Ayala F, Watson G, Otegui ME, and Chan RL
- Subjects
- Homeodomain Proteins metabolism, Plant Proteins metabolism, Plants, Genetically Modified genetics, Plants, Genetically Modified growth & development, Transcription Factors metabolism, Triticum genetics, Helianthus genetics, Homeodomain Proteins genetics, Plant Proteins genetics, Transcription Factors genetics, Triticum growth & development
- Abstract
HaHB4 is a sunflower transcription factor belonging to the homeodomain-leucine zipper I family whose ectopic expression in Arabidopsis triggers drought tolerance. The use of PCR to clone the HaHB4 coding sequence for wheat transformation caused unprogrammed mutations producing subtle differences in its activation ability in yeast. Transgenic wheat plants carrying a mutated version of HaHB4 were tested in 37 field experiments. A selected transgenic line yielded 6% more (P<0.001) and had 9.4% larger water use efficiency (P<0.02) than its control across the evaluated environments. Differences in grain yield between cultivars were explained by the 8% improvement in grain number per square meter (P<0.0001), and were more pronounced in stress (16% benefit) than in non-stress conditions (3% benefit), reaching a maximum of 97% in one of the driest environments. Increased grain number per square meter of transgenic plants was accompanied by positive trends in spikelet numbers per spike, tillers per plant, and fertile florets per plant. The gene transcripts associated with abiotic stress showed that HaHB4's action was not dependent on the response triggered either by RD19 or by DREB1a, traditional candidates related to water deficit responses. HaHB4 enabled wheat to show some of the benefits of a species highly adapted to water scarcity, especially in marginal regions characterized by frequent droughts., (© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.)
- Published
- 2019
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6. A sunflower WRKY transcription factor stimulates the mobilization of seed-stored reserves during germination and post-germination growth.
- Author
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Raineri J, Hartman MD, Chan RL, Iglesias AA, and Ribichich KF
- Subjects
- Abscisic Acid metabolism, Arabidopsis genetics, Carbohydrate Metabolism genetics, Carbon metabolism, Gene Expression Regulation, Plant, Genes, Plant, Gibberellins metabolism, Gluconeogenesis genetics, Helianthus genetics, Lipid Metabolism genetics, Models, Biological, Molecular Sequence Annotation, Oligonucleotide Array Sequence Analysis, Plant Leaves metabolism, Plant Proteins genetics, Plants, Genetically Modified, Seedlings metabolism, Seeds genetics, Seeds growth & development, Germination genetics, Helianthus growth & development, Helianthus metabolism, Plant Proteins metabolism, Seeds metabolism, Transcription Factors metabolism
- Abstract
Key Message: The sunflower transcription factor HaWRKY10 stimulates reserves mobilization in Arabidopsis. Gene expression and enzymes activity assays indicated that lipolysis and gluconeogenesis were increased. Microarray results suggested a parallelism in sunflower. Germinating oilseeds converts stored lipids into sugars, and thereafter in metabolic energy that is used in seedling growth and establishment. During germination, the induced lipolysis linked to the glyoxylate pathway and gluconeogenesis produces sucrose, which is then transported to the embryo and driven through catabolic routes. Herein, we report that the sunflower transcription factor HaWRKY10 regulates carbon partitioning by reducing carbohydrate catabolism and increasing lipolysis and gluconeogenesis. HaWRKY10 was regulated by abscisic acid and gibberellins in the embryo leaves 48 h after seed imbibition and highly expressed during sunflower seed germination and seedling growth, concomitantly with lipid mobilization. Sunflower leaf disks overexpressing HaWRKY10 showed repressed expression of genes related to sucrose cleavage and glycolysis compared with controls. Moreover, HaWRKY10 constitutive expression in Arabidopsis seeds produced higher decrease in lipid reserves, whereas starch and sucrose were more preserved compared with wild type. Gene transcripts abundance and enzyme activities involved in stored lipid mobilization and gluconeogenesis increased more in transgenic than in wild type seeds 36 h after imbibition, whereas the negative regulator of lipid mobilization, ABI4, was repressed. Altogether, the results point out a functional parallelism between tissues and plant species, and reveal HaWRKY10 as a positive regulator of storage reserve mobilization in sunflower.
- Published
- 2016
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7. The sunflower transcription factor HaWRKY76 confers drought and flood tolerance to Arabidopsis thaliana plants without yield penalty.
- Author
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Raineri J, Ribichich KF, and Chan RL
- Subjects
- Adaptation, Physiological, Arabidopsis genetics, Biomass, Droughts, Floods, Plant Proteins genetics, Plant Proteins metabolism, Plants, Genetically Modified, Promoter Regions, Genetic genetics, Seedlings genetics, Seedlings physiology, Seeds genetics, Seeds physiology, Stress, Physiological, Transcription Factors genetics, Arabidopsis physiology, Helianthus genetics, Models, Biological, Transcription Factors metabolism
- Abstract
Key Message: Arabidopsis transgenic plants expressing the sunflower transcription factor HaWRKY76 exhibit increased yield and tolerance to drought and flood stresses. The genetic construct containing HaWRKY76 is proposed as a potential biotechnological tool to improve crops. Water deficit and water excess are abiotic stress factors that seriously affect crops worldwide. To increase the tolerance to such stresses without causing yield penalty constitutes a major goal for biotechnologists. In this survey, we report that HaWRKY76, a divergent sunflower WRKY transcription factor, is able to confer both dehydration and submergence tolerance to Arabidopsis transgenic plants without yield penalty. The expression pattern of HaWRKY76 was analyzed in plants grown in standard conditions and under different watering regimes indicating a regulation by water availability. The corresponding cDNA was isolated and cloned under the control of a constitutive promoter and Arabidopsis plants were transformed with this construct. These transgenic plants presented higher biomass, seed production and sucrose content than controls in standard growth conditions. Moreover, they exhibited tolerance to mild drought or flood (complete submergence/waterlogging) stresses as well as the same or increased yield, depending on the stress severity and plant developmental stage, compared with controls. Drought tolerance occurred via an ABA-independent mechanism and induction of stomatal closure. Submergence tolerance can be explained by the carbohydrate (sucrose and starch) preservation achieved through the repression of fermentation pathways. Higher cell membrane stability and chlorenchyma maintenance could be the nexus between tolerance responses in front of both stresses. Altogether, the obtained results indicated that HaWRKY76 can be a potential biotechnological tool to improve crops yield as well as drought and flood tolerances.
- Published
- 2015
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8. Structural and thermodynamic studies of two centrin isoforms from Blastocladiella emersonii upon calcium binding.
- Author
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Camargo AI, Wiggers HJ, Damalio JC, Araujo AP, Ribichich KF, and de Camargo PC
- Subjects
- Blastocladiella metabolism, Calcium metabolism, Circular Dichroism, Fungal Proteins metabolism, Humans, Microscopy, Atomic Force, Protein Binding, Protein Isoforms chemistry, Protein Isoforms metabolism, Protein Structure, Secondary, Thermodynamics, Trimethoprim, Sulfamethoxazole Drug Combination metabolism, Blastocladiella chemistry, Calcium chemistry, Fungal Proteins chemistry, Protein Folding, Trimethoprim, Sulfamethoxazole Drug Combination chemistry
- Abstract
Centrins are calcium-binding proteins associated with microtubules organizing centers. Members of two divergent subfamilies of centrins were found in the aquatic fungus Blastocladiella emersonii, contrasting with the occurrence of only one member known for the better explored terrestrial fungi. BeCen1 shows greatest identity with human centrins HsCen1, HsCen2 and green algae centrin CrCenp, while BeCen3 records largest identity with human centrin HsCen3 and yeast centrin Cdc31p. Following the discovery of this unique feature, BeCen1 and BeCen3 centrins were produced to study whether these proteins had distinct features upon calcium binding. Circular dichroism showed opposite calcium binding effects on the α-helix arrangement of the secondary structure. The spectra indicated a decrease in α-helix signal for holo-BeCen1 contrasting with an increase for holo-BeCen3. In addition, only BeCen1 refolds after being de-natured. The fluorescence emission of the hydrophobic probe ANS increases for both proteins likely due to hydrophobic exposure, however, only BeCen1 presents a clear blue shift when calcium is added. ITC experiments identified four calcium binding sites for both proteins. In contrast to calcium binding to BeCen1, which is mainly endothermic, binding to BeCen3 is mainly exothermic. Light-scattering evidenced the formation of large particles in solution for BeCen1 and BeCen3 at temperatures above 30°C and 40°C, respectively. Atomic force microscopy confirmed the presence of supramolecular structures, which differ in the compactness and branching degree. Binding of calcium leads to different structural changes in BeCen1 and BeCen3 and the thermodynamic characteristics of the interaction also differ., (© 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
- Full Text
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9. The mitochondrial view of Blastocladiella emersonii.
- Author
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Tambor JH, Ribichich KF, and Gomes SL
- Subjects
- Adenine, Allomyces genetics, Blastocladiella classification, Codon genetics, DNA, Fungal isolation & purification, Gene Library, Genome, Fungal, Open Reading Frames, Phylogeny, Thymine, Blastocladiella genetics, DNA, Fungal genetics, DNA, Mitochondrial genetics
- Abstract
The mitochondrial genome of the chytrid Blastocladiella emersonii was sequenced and annotated, revealing the complete set of oxidative phosphorylation genes and tRNAs/rRNAs necessary for the translation process. Phylogenetic reconstructions reinforce the proposal of the new phylum Blastocladiomycota. Evidences of gene duplication due to inserted elements suggest shared susceptibility to gene invasion/exchange between chytrids and zygomycetes. The gene content of B. emersonii is very similar to Allomyces macrogynus but the content of intronic and changeable elements is much lower suggesting a stronger resistance to this kind of exchange. In addition, a total of 401 potential nuclear transcripts encoding mitochondrial proteins were obtained after B. emersonii EST database scanning using Saccharomyces cerevisiae, Homo sapiens and Arabidopsis thaliana data as probes and TargetP tool to find N-terminal mitochondrial signal in translated sequences.
- Published
- 2008
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10. Comparative EST analysis provides insights into the basal aquatic fungus Blastocladiella emersonii.
- Author
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Ribichich KF, Georg RC, and Gomes SL
- Subjects
- Animals, Conserved Sequence, DNA, Fungal analysis, DNA, Plant analysis, Databases, Genetic, Genetic Speciation, Humans, Methylmalonyl-CoA Decarboxylase genetics, Methylmalonyl-CoA Decarboxylase metabolism, Mice, Neurospora crassa genetics, Phylogeny, Rats, Sequence Homology, Nucleic Acid, Species Specificity, Ustilago genetics, Vitamin B 12 genetics, Vitamin B 12 metabolism, Blastocladiella genetics, Expressed Sequence Tags, Sequence Analysis, DNA
- Abstract
Background: Blastocladiella emersonii is an aquatic fungus of the Chytridiomycete class, which is at the base of the fungal phylogenetic tree. In this sense, some ancestral characteristics of fungi and animals or fungi and plants could have been retained in this aquatic fungus and lost in members of late-diverging fungal species. To identify in B. emersonii sequences associated with these ancestral characteristics two approaches were followed: (1) a large-scale comparative analysis between putative unigene sequences (uniseqs) from B. emersonii and three databases constructed ad hoc with fungal proteins, animal proteins and plant unigenes deposited in Genbank, and (2) a pairwise comparison between B. emersonii full-length cDNA sequences and their putative orthologues in the ascomycete Neurospora crassa and the basidiomycete Ustilago maydis., Results: Comparative analyses of B. emersonii uniseqs with fungi, animal and plant databases through the two approaches mentioned above produced 166 B. emersonii sequences, which were identified as putatively absent from other fungi or not previously described. Through these approaches we found: (1) possible orthologues of genes previously identified as specific to animals and/or plants, and (2) genes conserved in fungi, but with a large difference in divergence rate in B. emersonii. Among these sequences, we observed cDNAs encoding enzymes from coenzyme B12-dependent propionyl-CoA pathway, a metabolic route not previously described in fungi, and validated their expression in Northern blots., Conclusion: Using two different approaches involving comparative sequence analyses, we could identify sequences from the early-diverging fungus B. emersonii previously considered specific to animals or plants, and highly divergent sequences from the same fungus relative to other fungi.
- Published
- 2006
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11. Blastocladiella emersonii expresses a centrin similar to Chlamydomonas reinhardtii isoform not found in late-diverging fungi.
- Author
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Ribichich KF and Gomes SL
- Subjects
- Amino Acid Sequence, Animals, Blastocladiella genetics, Blastocladiella immunology, Calcium-Binding Proteins analysis, Calcium-Binding Proteins genetics, Chlamydomonas reinhardtii genetics, Chlamydomonas reinhardtii metabolism, Chromosomal Proteins, Non-Histone analysis, Chromosomal Proteins, Non-Histone genetics, Fungal Proteins analysis, Fungal Proteins genetics, Humans, Molecular Sequence Data, Phylogeny, Protein Isoforms analysis, Protein Isoforms genetics, Protein Isoforms metabolism, Protozoan Proteins genetics, Protozoan Proteins metabolism, Blastocladiella metabolism, Calcium-Binding Proteins metabolism, Chromosomal Proteins, Non-Histone metabolism, Fungal Proteins metabolism
- Abstract
Centrins are members of the calcium-binding EF-hand protein superfamily which can be divided into two subfamilies, probably associated with different functions: one related to Chlamydomonas reinhardtii centrin, CrCenp, and the other, represented by Saccharomyces cerevisiae isoform, ScCdc31p. ESTs encoding the two isoforms (BeCen1 and BeCen3) from the chytridiomycete Blastocladiella emersonii were isolated, and expression of the CrCenp-type centrin, BeCen1, was analyzed throughout the fungus life cycle. Becen1 mRNA levels increase transiently during sporulation and protein levels present a similar pattern. Immunolocalization studies seem to localize BeCen1 at the basal body zone and in the cytoplasm surrounding the nuclear cap, a zoospore organelle.
- Published
- 2005
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12. Gene discovery and expression profile analysis through sequencing of expressed sequence tags from different developmental stages of the chytridiomycete Blastocladiella emersonii.
- Author
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Ribichich KF, Salem-Izacc SM, Georg RC, Vêncio RZ, Navarro LD, and Gomes SL
- Subjects
- Amino Acid Sequence, Animals, Databases, Nucleic Acid, Genes, Fungal, Humans, Life Cycle Stages genetics, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Sequence Alignment, Blastocladiella genetics, Blastocladiella physiology, Expressed Sequence Tags, Gene Expression Profiling, Genome, Fungal, Sequence Analysis, DNA
- Abstract
Blastocladiella emersonii is an aquatic fungus of the chytridiomycete class which diverged early from the fungal lineage and is notable for the morphogenetic processes which occur during its life cycle. Its particular taxonomic position makes this fungus an interesting system to be considered when investigating phylogenetic relationships and studying the biology of lower fungi. To contribute to the understanding of the complexity of the B. emersonii genome, we present here a survey of expressed sequence tags (ESTs) from various stages of the fungal development. Nearly 20,000 cDNA clones from 10 different libraries were partially sequenced from their 5' end, yielding 16,984 high-quality ESTs. These ESTs were assembled into 4,873 putative transcripts, of which 48% presented no matches with existing sequences in public databases. As a result of Gene Ontology (GO) project annotation, 1,680 ESTs (35%) were classified into biological processes of the GO structure, with transcription and RNA processing, protein biosynthesis, and transport as prevalent processes. We also report full-length sequences, useful for construction of molecular phylogenies, and several ESTs that showed high similarity with known proteins, some of which were not previously described in fungi. Furthermore, we analyzed the expression profile (digital Northern analysis) of each transcript throughout the life cycle of the fungus using Bayesian statistics. The in silico approach was validated by Northern blot analysis with good agreement between the two methodologies.
- Published
- 2005
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13. Cell-type-specific expression of plant cytochrome c mRNA in developing flowers and roots.
- Author
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Ribichich KF, Tioni MF, Chan RL, and Gonzalez DH
- Subjects
- Gene Expression Regulation, Developmental, Helianthus cytology, Helianthus growth & development, In Situ Hybridization, Plant Roots cytology, Plant Roots growth & development, Plant Roots metabolism, Plant Stems cytology, Plant Stems growth & development, Plant Stems metabolism, RNA, Messenger genetics, Cytochrome c Group genetics, Gene Expression Regulation, Plant, Helianthus genetics, Transcription, Genetic
- Abstract
We have used RNA in situ hybridization to analyze the expression of transcripts encoding cytochrome c in different tissues and organs of sunflower (Helianthus annuus). Although northern-blot hybridization experiments indicate that the relative abundance of transcripts does not vary greatly, we have detected important changes in localization during flower development. Enhanced expression is observed in floral meristems as soon as they are discernible from the central portion of the capitulum containing the inflorescence meristem. As flowers develop, labeling is observed in all developing floral organ primordia. Later in development, expression in petals is reduced, and only the central portion of the flower becomes labeled. During the process of stamen formation, hybridization signals were obtained mainly in anthers. Less developed flowers at this stage showed expression through the archesporial tissue. During meiosis, the label was observed mainly in tapetal cells. Specific expression patterns, similar to those obtained for sunflower, were observed when Arabidopsis flowers were analyzed with a homologous cytochrome c probe. Specific patterns of expression were also observed in young sunflower roots. In this case, enhanced expression was detected in developing endodermis and pericycle and in protoxylem initials. We conclude that cell-specific mechanisms operate to regulate the abundance of cytochrome c encoding transcripts in different plant tissues. The overlap between the expression patterns of the nuclear encoded cytochrome c gene and some mitochondrial genes suggests the existence of coordinated mechanisms of expression.
- Published
- 2001
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14. Histopathological Spikelet Changes Produced by Fusarium graminearum in Susceptible and Resistant Wheat Cultivars.
- Author
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Ribichich KF, Lopez SE, and Vegetti AC
- Abstract
Scab is an important disease of wheat throughout the world. Fusarium graminearum is the species most frequently isolated in Argentina. Histopathological modifications observed in Pro INTA Oasis (a susceptible Argentine cultivar) and in Sumai 3 (a resistant Chinese cultivar) from the time of inoculation, at the spike emergence stage, until major disease expression (10.5 days postinoculation in the susceptible cultivar) are described. Both cultivars showed a horizontal progression of disease, from anthers to glumes, and a vertical progression, from anthers to rachis. Chlorenchyma was the most damaged tissue, with progressive chlorosis and necrosis. Walls of mesophyll, parenchyma, and epidermal cells around the lesions became thicker, particularly in the resistant cultivar. Vessels and sieve tubes were occluded, particularly the former in the susceptible cultivar and the latter in the resistant cultivar. Possible relationships between tissue changes generated during disease progression and known resistance mechanisms are discussed.
- Published
- 2000
- Full Text
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