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22 results on '"Ribera D'Alcalà G."'

2. Come controllare la contaminazione da Legionella in una struttura ospedaliera

Author

Ribera d’Alcalà G., Montegrosso S., PENNINO, FRANCESCA, Iervolino C., Albanese Z., Russo I., Galdieri A., TORRE, IDA, TRIASSI, MARIA, Ribera d’Alcalà, G., Montegrosso, S., Pennino, Francesca, Iervolino, C., Albanese, Z., Russo, I., Galdieri, A., Torre, Ida, and Triassi, Maria

Published
2007

7. Lo studio multicentrico italiano sulle legionellosi: ricerche ambientali, sorveglianza epidemiologica, strategie di prevenzione e controllo

Author

Borella P., Neglia R., Montagna M.T., Napoli C., Pignato S., Giammanco G., Romano Spica V., Boccia S., Stancanelli G., Scaltriti S., Triassi M., Ribera D'Alcalà G., Zotti C.M., Ruggenini Moiraghi A., LEGNANI, PIER PAOLO, ZANETTI, FRANCA, Borella P., Neglia R., Montagna MT., Napoli C., Pignato S., Giammanco G., Romano Spica V., Boccia S., Legnani P., Zanetti F., Stancanelli G., Scaltriti S., Triassi M., Ribera D'Alcalà G., Zotti CM., and Ruggenini Moiraghi A.

Published
2004

8. Confronto tra diverse metodiche utilizzate per l'identificazione dell'antigene urinario di Legionella

Author

Montagna M.T., Tatò D., Spilotros G., Borella P., Stancanelli G., Triassi M., Zotti C.M., Marchesi I., Bargellini A., Scaltriti S., Ieri R., Ribera D'Alcalà G., Montegrosso S., Ditommaso S., Gentile M., LEONI, ERICA, LAZZARI, CLAUDIO, APORTI, MANUELA, Montagna MT., Tatò D., Spilotros G., Borella P., Leoni E., Stancanelli G., Triassi M., Zotti CM., Marchesi I., Bargellini A., Lazzari C., Aporti M., Scaltriti S., Ieri R., Ribera D'Alcalà G., Montegrosso S., Ditommaso S., and Gentile M.

Subjects
LEGIONELLA, ANTIGENE URINARIO
Published
2004

12. [Environmental diffusion of Legionella spp and legionellosis frequency among patients with pneumonia: preliminary results of a multicentric Italian survey]

Author

Borella, P, Montagna, M. T, Romano Spica, V, Stampi, S, Stancanelli, G, Triassi, M, Bargellini, A, Giacobazzi, P, Vercilli, F, Scaltriti, S, Marchesi, I, Napoli, Christian, Tatò, D, Spilotros, G, Paglionico, N, Quaranta, G, Branca, M, Tumbarello, M, Laurenti, P, Moscato, U, Capoluongo, E, De Luca, G, Legnani, P. P, Leoni, E, Sacchetti, R, Zanetti, F, Moro, M, Ossi, C, Lopalco, L, Santarpia, R, Conturso, V, Ribera d'Alcalà, G, and Montegrosso, S.

Subjects
Legionellosis, Italy, Humans, Legionella, Pneumonia, Bacterial, Water Microbiology, Bacterial, Pneumonia
Abstract

A multicentric Italian investigation on legionnaires' disease is in course to clarify host factors as well as pathogen associated characteristics involved in the infection/disease. The main goal of the research plan is to account for some critical aspects concerning identification and prevention of legionellosis. To improve knowledge on factors associated with Legionella spp colonisation in hot waters, to detect cases and to characterize risk factors in subjects which develop pneumonia are specific objectives of the research programme. Preliminary results show that hot waters of houses and hotels are frequently contaminated (22.6% and 54.6%, respectively), mainly by L. pneumophila. Microbial concentrations were low in domestic waters (1.000 ufc/l), but higher in samples from the hotels (geom. mean 1.85 x 10(3) ufc/l). Warming system, age of the plant, type of building were risk factors significantly associated with Legionella spp positivity. The active surveillance on patients affected by pneumonia with search for Legionella urinary antigen allowed the identification of 34 cases, 3 of which of nosocomial origin, corresponding to 4.2% of the screened pneumonia. After informed consent, 26 subjects were recruited for a case-control-study to clarify risk factors for the disease.

Published
2004

13. Indagine multicentrica sui fattori di rischio correlati alla presenza di Legionella spp nell’acqua di strutture pubbliche e private

Author

Borella, Paola, Montagna, M. T., Stampi, S., Stancanelli, G., Romano Spica, V., Triassi, M., Bargellini, Annalisa, Marchesi, Isabella, Tatò, D., Spilotros, G., De Luca, G., Sacchetti, R., Ossi, C., Nizzero, P., Laurenti, P., Moscato, U., Ribera D'alcalà, G., and Montegrosso, S.

Subjects
ospedali, Legionella spp, diffusione ambientale, case, alberghi, fattori di rischio
Published
2004

14. LO STUDIO MULTICENTRICO ITALIANO SULLE LEGIONELLOSI: RICERCHE AMBIENTALI, SORVEGLIANZA EPIDEMIOLOGICA, STRATEGIE DI PREVENZIONE E CONTROLLO

Author

Borella, Paola, Neglia, Rachele Giovanna, Montagna, M. T., Napoli, C., Pignato, S., Giammanco, G., Romano Spica, V., Boccia, S., Legnani, P., Zanetti, F., Stancanelli, G., Scaltriti, Stefania, Triassi, M., Ribera D’Alcalà, G., Zotti, C. M., and Ruggenini Moiraghi, A.

Subjects
anticorpi anti-Legionella, sorveglianza clinica ed ambientale, genotipizzazione, macrofagi, Legionella, studio epidemiologico, interazione Legionella-amebe, legionellosi, fattori di rischio, sierotipizzazione
Published
2004

17. Clinical and environmental distribution of Legionella pneumophila in a university hospital in Italy: efficacy of ultraviolet disinfection

Author

M. Crispino, S. Montegrosso, Susanna Cuccurullo, Raffaele Zarrilli, Paola Borella, A. Di Popolo, G. Ribera D’Alcalà, Z. Albanese, Maria Triassi, Triassi, M, Di Popolo, A, Ribera D'Alcalà, G, Albanese, Z, Cuccurullo, S, Montegrosso, S, Crispino, M, Borella, P, and Zarrilli, R.

Subjects
Microbiology (medical), Serotype, Neonatal intensive care unit, Legionella, Ultraviolet Rays, urinary antigen detection, Legionella pneumophila, serotyping, PFGE typing, UV disinfection, Microbiology, Hospitals, University, Water Supply, Pulsed-field gel electrophoresis, Medicine, Infection control, Humans, Serotyping, Cross Infection, Infection Control, Molecular Epidemiology, biology, Molecular epidemiology, business.industry, General Medicine, bacterial infections and mycoses, biology.organism_classification, medicine.disease, respiratory tract diseases, Electrophoresis, Gel, Pulsed-Field, Intensive Care Units, Infectious Diseases, Italy, bacteria, Legionnaires' disease, Legionnaires' Disease, business, Urinary antigen detection
Abstract

The molecular epidemiology of Legionella pneumophila in the 'V. Monaldi' University Hospital was studied. Seven cases of nosocomial Legionnaires' disease were diagnosed between 1999 and 2003. Two clinical legionella strains obtained from two patients in the adult cardiac surgery unit (CSU) and 30 environmental legionella strains from the paediatric and adult CSUs, neonatal intensive care unit (NICU) and the cardiorespiratory intensive care unit (CR-ICU) were serotyped and genotyped. L. pneumophila serogroup 1/Philadelphia with an identical pulsed-field gel electrophoresis (PFGE) profile A was isolated from two patients in the adult CSU, and from three and one water samples taken in the adult CSU and the paediatric CSU, respectively, from 2001 to 2002. Furthermore, L. pneumophila serogroup 3 with an identical PFGE profile B was identified in 20 environmental strains from all wards, L. pneumophila serogroup 3 with PFGE profile C was identified in a single environmental strain from the CR-ICU, and non-pneumophila Legionella with identical PFGE profile D was identified in five environmental strains from the adult CSU, paediatric CSU and NICU. Ultraviolet irradiation was effective in disinfection of the hospital water supplies in the adult and paediatric CSUs contaminated by L. pneumophila clone associated with nosocomial Legionnaires' disease. In conclusion, these data demonstrate that two cases of nosocomial legionellosis were caused by the persistence of a single clone of L. pneumophila serogroup 1/Philadelphia in the hospital environment, and that disinfection by ultraviolet irradiation may represent an effective measure to prevent nosocomial Legionnaires' disease. © 2005 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.

Published
2005

18. Legionella Infection Risk from Domestic Hot Water

Author

M. Moro, M. Teresa Montagna, Rachele Giovanna Neglia, Paola Borella, Gabriella Ribera D’Alcalà, Christian Napoli, D. Tatò, Patrizia Laurenti, Serena Stampi, Vincenzo Romano-Spica, Giovanna De Luca, G. Stancanelli, Isabella Marchesi, Guglielmina Fantuzzi, Cristina Ossi, Maria Triassi, Erica Leoni, Gianluigi Quaranta, Triassi, Maria, Borella, P, Montagna, Mt, ROMANO SPICA, V, Stampi, S, Stancanelli, G, Neglia, R, Marchesi, I, Fantuzzi, G, Tato, D, Napoli, C, Quaranta, G, Laurenti, P, Leoni, E, DE LUCA, G, Ossi, C, Moro, M, RIBERA D'ALCALA, G., Borella P., Montagna MT., Romano Spica V., Stampi S., Stancanelli G., Triassi M., Neglia R., Marchesi I., Fantuzzi G., Tatò D., Napoli C., Quaranta G., Laurenti P., Leoni E., De Luca G., Ossi C., Moro M., and Ribera D'Alcalà G.

Subjects
Microbiology (medical), Veterinary medicine, Infection risk, Epidemiology, Legionella, Hot water, HOT WATER SYSTEMS, chemistry.chemical_element, lcsh:Medicine, Legionella pneumophila, Microbiology, lcsh:Infectious and parasitic diseases, Risk Factors, Water Supply, community legionellosis, Pseudomonas, medicine, Chlorine, Humans, lcsh:RC109-216, Legionella multicentric survey, Legionellosis, biology, Legionella spp, Research, lcsh:R, Contamination, biology.organism_classification, medicine.disease, bacterial infections and mycoses, respiratory tract diseases, Infectious Diseases, Heater type, domestic hot water systems home colonization, chemistry, Italy, Metals, Pseudomonas spp, Legionnaires' disease, Legionnaires' Disease, Infection, Water Microbiology, water microbial contamination
Abstract

We investigated Legionella and Pseudomonas contamination of hot water in a cross-sectional multicentric survey in Italy. Chemical parameters (hardness, free chlorine, and trace elements) were determined. Legionella spp. were detected in 33 (22.6%) and Pseudomonas spp. in 56 (38.4%) of 146 samples. Some factors associated with Legionella contamination were heater type, tank distance and capacity, water plant age, and mineral content. Pseudomonas presence was influenced by water source, hardness, free chlorine, and temperature. Legionella contamination was associated with a centralized heater, distance from the heater point >10 m, and a water plant >10 years old. Furthermore, zinc levels of 50 microg/L appeared to be protective against Legionella colonization [corrected]. Legionella species and serogroups were differently distributed according to heater type, water temperature, and free chlorine, suggesting that Legionella strains may have a different sensibility and resistance to environmental factors and different ecologic niches.

19. Clinical and environmental distribution of Legionella pneumophila in a university hospital in Italy: efficacy of ultraviolet disinfection.

Author

Triassi M, Di Popolo A, Ribera D'Alcalà G, Albanese Z, Cuccurullo S, Montegrosso S, Crispino M, Borella P, and Zarrilli R

Subjects
Cross Infection transmission, Electrophoresis, Gel, Pulsed-Field, Hospitals, University, Humans, Intensive Care Units, Italy, Legionella pneumophila classification, Legionella pneumophila genetics, Legionnaires' Disease transmission, Molecular Epidemiology, Serotyping, Ultraviolet Rays, Water Supply, Cross Infection microbiology, Infection Control methods, Legionella pneumophila isolation & purification, Legionnaires' Disease microbiology
Abstract

The molecular epidemiology of Legionella pneumophila in the 'V. Monaldi' University Hospital was studied. Seven cases of nosocomial Legionnaires' disease were diagnosed between 1999 and 2003. Two clinical legionella strains obtained from two patients in the adult cardiac surgery unit (CSU) and 30 environmental legionella strains from the paediatric and adult CSUs, neonatal intensive care unit (NICU) and the cardiorespiratory intensive care unit (CR-ICU) were serotyped and genotyped. L. pneumophila serogroup 1/Philadelphia with an identical pulsed-field gel electrophoresis (PFGE) profile A was isolated from two patients in the adult CSU, and from three and one water samples taken in the adult CSU and the paediatric CSU, respectively, from 2001 to 2002. Furthermore, L. pneumophila serogroup 3 with an identical PFGE profile B was identified in 20 environmental strains from all wards, L. pneumophila serogroup 3 with PFGE profile C was identified in a single environmental strain from the CR-ICU, and non-pneumophila Legionella with identical PFGE profile D was identified in five environmental strains from the adult CSU, paediatric CSU and NICU. Ultraviolet irradiation was effective in disinfection of the hospital water supplies in the adult and paediatric CSUs contaminated by L. pneumophila clone associated with nosocomial Legionnaires' disease. In conclusion, these data demonstrate that two cases of nosocomial legionellosis were caused by the persistence of a single clone of L. pneumophila serogroup 1/Philadelphia in the hospital environment, and that disinfection by ultraviolet irradiation may represent an effective measure to prevent nosocomial Legionnaires' disease.

Published
2006
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20. Legionella contamination in hot water of Italian hotels.

Author

Borella P, Montagna MT, Stampi S, Stancanelli G, Romano-Spica V, Triassi M, Marchesi I, Bargellini A, Tatò D, Napoli C, Zanetti F, Leoni E, Moro M, Scaltriti S, Ribera D'Alcalà G, Santarpia R, and Boccia S

Subjects
Colony Count, Microbial, Fresh Water chemistry, Italy, Legionella classification, Legionella genetics, Legionella pneumophila classification, Legionella pneumophila genetics, Legionella pneumophila isolation & purification, Regression Analysis, Serotyping, Fresh Water microbiology, Hot Temperature, Legionella isolation & purification, Travel, Water Pollution, Water Supply
Abstract

A cross-sectional multicenter survey of Italian hotels was conducted to investigate Legionella spp. contamination of hot water. Chemical parameters (hardness, free chlorine concentration, and trace element concentrations), water systems, and building characteristics were evaluated to study risk factors for colonization. The hot water systems of Italian hotels were strongly colonized by Legionella; 75% of the buildings examined and 60% of the water samples were contaminated, mainly at levels of > or =10(3) CFU liter(-1), and Legionella pneumophila was the most frequently isolated species (87%). L. pneumophila serogroup 1 was isolated from 45.8% of the contaminated sites and from 32.5% of the hotels examined. When a multivariate logistic model was used, only hotel age was associated with contamination, but the risk factors differed depending on the contaminating species and serogroup. Soft water with higher chlorine levels and higher temperatures were associated with L. pneumophila serogroup 1 colonization, whereas the opposite was observed for serogroups 2 to 14. In conclusion, Italian hotels, particularly those located in old buildings, represent a major source of risk for Legionnaires' disease due to the high frequency of Legionella contamination, high germ concentration, and major L. pneumophila serogroup 1 colonization. The possible role of chlorine in favoring the survival of Legionella species is discussed.

Published
2005
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21. Legionella infection risk from domestic hot water.

Author

Borella P, Montagna MT, Romano-Spica V, Stampi S, Stancanelli G, Triassi M, Neglia R, Marchesi I, Fantuzzi G, Tatò D, Napoli C, Quaranta G, Laurenti P, Leoni E, De Luca G, Ossi C, Moro M, and Ribera D'Alcalà G

Subjects
Humans, Italy, Risk Factors, Water Microbiology, Legionella isolation & purification, Legionella pneumophila isolation & purification, Legionellosis etiology, Legionnaires' Disease etiology, Metals analysis, Pseudomonas isolation & purification, Water Supply analysis
Abstract

We investigated Legionella and Pseudomonas contamination of hot water in a cross-sectional multicentric survey in Italy. Chemical parameters (hardness, free chlorine, and trace elements) were determined. Legionella spp. were detected in 33 (22.6%) and Pseudomonas spp. in 56 (38.4%) of 146 samples. Some factors associated with Legionella contamination were heater type, tank distance and capacity, water plant age, and mineral content. Pseudomonas presence was influenced by water source, hardness, free chlorine, and temperature. Legionella contamination was associated with a centralized heater, distance from the heater point >10 m, and a water plant >10 years old. Furthermore, zinc levels of <100 microg/L and copper levels of >50 microg/L appeared to be protective against Legionella colonization [corrected]. Legionella species and serogroups were differently distributed according to heater type, water temperature, and free chlorine, suggesting that Legionella strains may have a different sensibility and resistance to environmental factors and different ecologic niches.

Published
2004
Full Text
View/download PDF

22. [Environmental diffusion of Legionella spp and legionellosis frequency among patients with pneumonia: preliminary results of a multicentric Italian survey].

Author

Borella P, Montagna MT, Romano-Spica V, Stampi S, Stancanelli G, Triassi M, Bargellini A, Giacobazzi P, Vercilli F, Scaltriti S, Marchesi I, Napoli C, Tatò D, Spilotros G, Paglionico N, Quaranta G, Branca M, Tumbarello M, Laurenti P, Moscato U, Capoluongo E, De Luca G, Legnani PP, Leoni E, Sacchetti R, Zanetti F, Moro M, Ossi C, Lopalco L, Santarpia R, Conturso V, Ribera d'Alcalà G, and Montegrosso S

Subjects
Humans, Italy epidemiology, Pneumonia, Bacterial microbiology, Legionella isolation & purification, Legionellosis epidemiology, Pneumonia, Bacterial epidemiology, Water Microbiology
Abstract

A multicentric Italian investigation on legionnaires' disease is in course to clarify host factors as well as pathogen associated characteristics involved in the infection/disease. The main goal of the research plan is to account for some critical aspects concerning identification and prevention of legionellosis. To improve knowledge on factors associated with Legionella spp colonisation in hot waters, to detect cases and to characterize risk factors in subjects which develop pneumonia are specific objectives of the research programme. Preliminary results show that hot waters of houses and hotels are frequently contaminated (22.6% and 54.6%, respectively), mainly by L. pneumophila. Microbial concentrations were low in domestic waters (<1.000 ufc/l), but higher in samples from the hotels (geom. mean 1.85 x 10(3) ufc/l). Warming system, age of the plant, type of building were risk factors significantly associated with Legionella spp positivity. The active surveillance on patients affected by pneumonia with search for Legionella urinary antigen allowed the identification of 34 cases, 3 of which of nosocomial origin, corresponding to 4.2% of the screened pneumonia. After informed consent, 26 subjects were recruited for a case-control-study to clarify risk factors for the disease.

Published
2003

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