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10 results on '"Ribeiro-Samy, Silvina"'

3. Unveiling the Differences of Secretome of Human Bone Marrow Mesenchymal Stem Cells, Adipose Tissue-Derived Stem Cells, and Human Umbilical Cord Perivascular Cells: A Proteomic Analysis

Author

Pires, Ana O., primary, Mendes-Pinheiro, Barbara, additional, Teixeira, Fábio G., additional, Anjo, Sandra I., additional, Ribeiro-Samy, Silvina, additional, Gomes, Eduardo D., additional, Serra, Sofia C., additional, Silva, Nuno A., additional, Manadas, Bruno, additional, Sousa, Nuno, additional, and Salgado, Antonio J., additional

Published
2016
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6. Development and Characterization of a PHB‐HV‐based 3D Scaffold for a Tissue Engineering and Cell‐therapy Combinatorial Approach for Spinal Cord Injury Regeneration

Author

Ribeiro‐Samy, Silvina, primary, Silva, Nuno A., additional, Correlo, Vitor M., additional, Fraga, Joana S., additional, Pinto, Luísa, additional, Teixeira‐Castro, Andreia, additional, Leite‐Almeida, Hugo, additional, Almeida, Armando, additional, Gimble, Jeffrey M., additional, Sousa, Nuno, additional, Salgado, António J., additional, and Reis, Rui L., additional

Published
2013
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10. In vitro evaluation of cell-seeded chitosan films for peripheral nerve tissue engineering.

Author

Wrobel S, Serra SC, Ribeiro-Samy S, Sousa N, Heimann C, Barwig C, Grothe C, Salgado AJ, and Haastert-Talini K

Subjects
Animals, Animals, Newborn, Cell Differentiation, Cell Proliferation, Cells, Cultured, Equipment Design, Equipment Failure Analysis, Guided Tissue Regeneration instrumentation, Mesenchymal Stem Cells physiology, Nerve Regeneration physiology, Neurons physiology, Peripheral Nerves growth & development, Rats, Rats, Wistar, Schwann Cells physiology, Chitosan chemistry, Mesenchymal Stem Cells cytology, Neurons cytology, Peripheral Nerves cytology, Schwann Cells cytology, Tissue Engineering instrumentation, Tissue Scaffolds
Abstract

Natural biomaterials have attracted an increasing interest in the field of tissue-engineered nerve grafts, representing a possible alternative to autologous nerve transplantation. With the prospect of developing a novel entubulation strategy for transected nerves with cell-seeded chitosan films, we examined the biocompatibility of such films in vitro. Different types of rat Schwann cells (SCs)--immortalized, neonatal, and adult-of the chitosan substrate. Both cell types were viable on the biomaterial and showed different metabolic activities and proliferation behavior, indicating cell-type-specific cell-biomaterial interaction. Moreover, the cell types also displayed their typical morphology. In cocultures adult SCs used the BMSCs as a feeder layer and no negative interactions between both cell types were detected. Further, the chitosan films allow neurite outgrowth from dissociated sensory neurons, which is additionally supported on film preseeded with SC-BMSC cocultures. The presented chitosan films therefore demonstrate high potential for their use in tissue-engineered nerve grafts.

Published
2014
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