1. Photoreceptor Outer Segment Isolation from a Single Canine Retina for RPE Phagocytosis Assay.
- Author
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Sudharsan R, Elliott MH, Dolgova N, Aguirre GD, and Beltran WA
- Subjects
- Animals, Cells, Cultured, Dogs, Fluorescent Antibody Technique, Direct, Fluorescent Dyes, Rhodopsin analysis, Rhodopsin immunology, Rod Cell Outer Segment, Staining and Labeling methods, Zonula Occludens-1 Protein analysis, Zonula Occludens-1 Protein immunology, Cell Fractionation methods, Phagocytosis, Primary Cell Culture methods, Retina cytology, Retinal Pigment Epithelium metabolism
- Abstract
Protocols for photoreceptor outer segment (POS) isolation that can be used in phagocytosis assays of retinal pigment epithelium (RPE) cells have routinely used a large number of cow or pig eyes. However, when working with large animal models (e.g., dog, cats, transgenic pigs) of inherited retinal degenerative diseases, access to retinal tissues may be limited. An optimized protocol is presented in this paper to isolate sufficient POS from a single canine retina for use in RPE phagocytosis assays.
- Published
- 2018
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