1. Engineering an Ancestral Glycosyltransferase for Biosynthesis of 2-Phenylethyl-β-d-Glucopyranoside and Salidroside.
- Author
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Zhang T, Tian S, Gao Z, Li Y, and Jia H
- Subjects
- Molecular Docking Simulation, Enzyme Stability, Kinetics, Phenylethyl Alcohol metabolism, Phenylethyl Alcohol chemistry, Phenylethyl Alcohol analogs & derivatives, Protein Engineering, Plant Proteins genetics, Plant Proteins metabolism, Plant Proteins chemistry, Rhodiola chemistry, Rhodiola genetics, Rhodiola enzymology, Rhodiola metabolism, Glucosides chemistry, Glucosides metabolism, Glucosides biosynthesis, Glycosyltransferases genetics, Glycosyltransferases metabolism, Glycosyltransferases chemistry, Phenols metabolism, Phenols chemistry
- Abstract
Phenylethanoid glycosides (PhGs) are naturally occurring glycosides derived from plants with various biological activities. Glycosyltransferases catalyze the production of PhGs from phenylethanols via a transglycosylation reaction. The low activity and stability of glycosyltransferase limit its industrial application. An ancestral glycosyltransferase, UGTAn85, with heat resistance, alkali resistance, and high stability was resurrected using ancestral sequence reconstruction technology. This enzyme can efficiently convert phenylethanols to PhGs. The optimal reaction temperature and pH for UGTAn85 were found to be 70 °C and pH 10.0, respectively. This study employed a combination of structure-guided rational design and co-evolution analysis to enhance its catalytic activity. Potential mutation sites were identified through computer-aided design, including homology modeling, molecular docking, Rosetta dock design, molecular dynamics simulation, and co-evolution analysis. By targeted mutagenesis, the UGTAn85 mutant Q23E/N65D exhibited a 2.2-fold increase in enzyme activity (11.85 U/mg) and elevated affinity ( K
m = 0.11 mM) for 2-phenylethanol compared to UGTAn85. Following a fed-batch reaction, 36.16 g/L 2-phenylethyl-β-d-glucopyranoside and 51.49 g/L salidroside could be produced within 24 h, respectively. The findings in this study provide a new perspective on enhancing the stability and activity of glycosyltransferases, as well as a potential biocatalyst for the industrial production of PhGs.- Published
- 2024
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